pycom04g03640

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013958
Physical Location & Seq
Forward (+)
3278536 .. 3279207
672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03640.1

Sequence Viewer

Length: 672 bp
ATGCCAACCATTTTCCGAAGGATTTTCCTTATTTACAATCTTCTTAATACATTCCTCCTCTCTTTGGTCCCCAAGCATCTCAGGCCTCTCCTCCCCTCCTCCTGGTTCCCCCACCACAGCACTCTTCTTGACACCAAAACACCGTCACCCCAACCCCCACCTCCGTCGTTGTTGTCTTTGCCCTTGCCCTTGCCTTCTGGCGGTGCTTGTCACGTCAGAATGGACCCCAATGAGCTCAAACGCGTGTTCCAGATGTTTGACCGCAATGGGGACGGGCGGATCACCAAGCAGGAGCTCAACGACTCCTTGGAGAACTTGGGAATCTACATCCCCGACAAGGAGCTCTTCAACATGATCGAGAAGATTGACGTGAACGGTGACGGGTGCGTGGACATTGACGAGTTCGGGGAGTTGTACCAGTCTATCATGGACGAGCGCGACGAGGAGGAGGACATGAAGGAGGCATTCAACGTGTTTGATCAAAACGGAGACGGGTTCATAACCGTCGATGAGTTGAGGTCGGTTTTGTCGTCCCTCGGGCTTAAGCAAGGGAGAACTATAGAGGACTGCAAGAGGATGATCATGAAGGTGGATGTGGACGGAGATGGGAGGGTCAACTACAAGGAATTCAGGCAAATGATGAAGGGAGGAGGGTTTAGTGCATTGAGTTAA

Protein Analysis

224

Amino Acids

25.37

Weight (kDa)

4.91

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 77 - 140 5e-14 EF-hand domain pair
EF-hand_1 PF00036 78 - 101 5.6e-07 EF hand domain
EF-hand_6 PF13405 78 - 107 1.8e-07 EF-hand domain
EF-hand_5 PF13202 79 - 100 3e-07 EF hand
AIF-1 PF21008 143 - 217 5.6e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 149 - 215 2.4e-16 EF-hand domain pair
EF-hand_6 PF13405 151 - 180 2.1e-09 EF-hand domain
EF-hand_1 PF00036 152 - 179 2.1e-08 EF hand domain
EF-hand_5 PF13202 153 - 175 1.4e-07 EF hand
EF-hand_1 PF00036 189 - 215 3e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 243, 438
AciI CCGC 3 cut(s) 201, 262, 277
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 1 cut(s) 626
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 5 cut(s) 64, 102, 200, 268, 337
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 2 cut(s) 241, 471
AgsI TTSAA 2 cut(s) 349, 469
AjiI CACGTC 2 cut(s) 214, 370
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 3 cut(s) 235, 295, 343
AluI AGCT 3 cut(s) 235, 295, 343
Alw21I GWGCWC 3 cut(s) 237, 297, 345
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 626
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 67, 223
AsuHPI GGTGA 3 cut(s) 138, 274, 389
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 67, 223
BanII GRGCYC 3 cut(s) 237, 297, 345
Bbv12I GWGCWC 3 cut(s) 237, 297, 345
BccI CCATC 1 cut(s) 599
BciT130I CCWGG 1 cut(s) 103
BclI TGATCA 2 cut(s) 478, 579
BcoDI GTCTC 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 542
Bme1390I CCNGG 1 cut(s) 103
Bme18I GGWCC 2 cut(s) 67, 223
BmeT110I CYCGRG 1 cut(s) 536
BmgBI CACGTC 2 cut(s) 214, 370
BmgT120I GGNCC 2 cut(s) 67, 223
BmiI GGNNCC 3 cut(s) 69, 107, 225
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 306, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 102, 200, 268, 337
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 306, 535
BseGI GGATG 3 cut(s) 327, 582, 598
BseLI CCNNNNNNNGG 5 cut(s) 64, 102, 200, 268, 337
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 6 cut(s) 47, 80, 88, 458, 461, 663
Bsh1236I CGCG 2 cut(s) 243, 438
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 3 cut(s) 237, 297, 345
BsiHKCI CYCGRG 1 cut(s) 536
BslFI GGGAC 3 cut(s) 53, 284, 517
BslI CCNNNNNNNGG 5 cut(s) 64, 102, 200, 268, 337
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 3 cut(s) 53, 284, 517
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 3 cut(s) 237, 297, 345
Bsp143I GATC 4 cut(s) 279, 354, 478, 579
BspACI CCGC 3 cut(s) 201, 262, 277
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 93
BspFNI CGCG 2 cut(s) 243, 438
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 3 cut(s) 69, 107, 225
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 271
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 306, 535
BssMI GATC 4 cut(s) 279, 354, 478, 579
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 3 cut(s) 144, 377, 505
Bst6I CTCTTC 2 cut(s) 129, 350
BstAFI CTTAAG 1 cut(s) 542
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 327, 582, 598
BstFNI CGCG 2 cut(s) 243, 438
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 4 cut(s) 282, 357, 481, 582
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 4 cut(s) 279, 354, 478, 579
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 2 cut(s) 243, 438
BsuRI GGCC 1 cut(s) 85
BtrI CACGTC 2 cut(s) 214, 370
BtsCI GGATG 3 cut(s) 327, 582, 598
CciI TCATGA 1 cut(s) 582
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 67, 223
Csp6I GTAC 1 cut(s) 415
CviAII CATG 4 cut(s) 352, 427, 454, 583
CviJI RGCY 5 cut(s) 85, 235, 295, 343, 541
CviKI_1 RGCY 5 cut(s) 85, 235, 295, 343, 541
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 1 cut(s) 80
DpnI GATC 4 cut(s) 281, 356, 480, 581
DpnII GATC 4 cut(s) 279, 354, 478, 579
Eam1104I CTCTTC 2 cut(s) 129, 350
EarI CTCTTC 2 cut(s) 129, 350
EciI GGCGGA 1 cut(s) 292
Ecl136II GAGCTC 3 cut(s) 235, 295, 343
Eco130I CCWWGG 1 cut(s) 306
Eco147I AGGCCT 1 cut(s) 85
Eco24I GRGCYC 3 cut(s) 237, 297, 345
Eco47I GGWCC 2 cut(s) 67, 223
Eco53kI GAGCTC 3 cut(s) 235, 295, 343
Eco88I CYCGRG 1 cut(s) 536
EcoICRI GAGCTC 3 cut(s) 235, 295, 343
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 3 cut(s) 237, 297, 345
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 4 cut(s) 355, 430, 457, 586
FaiI YATR 6 cut(s) 353, 428, 455, 500, 560, 584
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 53, 284, 517
FatI CATG 4 cut(s) 351, 426, 453, 582
FbaI TGATCA 2 cut(s) 478, 579
FokI GGATG 3 cut(s) 314, 589, 605
FriOI GRGCYC 3 cut(s) 237, 297, 345
GlaI GCGC 1 cut(s) 437
HaeIII GGCC 1 cut(s) 85
HhaI GCGC 1 cut(s) 438
Hin1II CATG 4 cut(s) 355, 430, 457, 586
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HinfI GANTC 2 cut(s) 302, 321
HphI GGTGA 3 cut(s) 138, 274, 389
Hpy166II GTNNAC 4 cut(s) 373, 391, 598, 616
Hpy188I TCNGA 2 cut(s) 17, 218
Hpy188III TCNNGA 4 cut(s) 128, 250, 358, 583
Hpy8I GTNNAC 4 cut(s) 373, 391, 598, 616
Hpy99I CGWCG 3 cut(s) 169, 443, 509
HpyAV CCTTC 5 cut(s) 12, 204, 451, 580, 637
HpyCH4III ACNGT 3 cut(s) 144, 377, 505
HpyCH4IV ACGT 3 cut(s) 213, 369, 471
HpyCH4V TGCA 2 cut(s) 570, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 3 cut(s) 213, 369, 471
Hsp92II CATG 4 cut(s) 355, 430, 457, 586
HspAI GCGC 1 cut(s) 436
Ksp22I TGATCA 2 cut(s) 478, 579
Kzo9I GATC 4 cut(s) 279, 354, 478, 579
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 292, 340
LpnPI CCDG 8 cut(s) 67, 88, 115, 183, 263, 275, 431, 616
LweI GCATC 1 cut(s) 85
MaeII ACGT 3 cut(s) 213, 369, 471
MaeIII GTNAC 3 cut(s) 144, 209, 377
MalI GATC 4 cut(s) 281, 356, 480, 581
MboI GATC 4 cut(s) 279, 354, 478, 579
MboII GAAGA 4 cut(s) 32, 116, 337, 373
MhlI GDGCHC 3 cut(s) 237, 297, 345
MluCI AATT 1 cut(s) 626
MluI ACGCGT 1 cut(s) 241
MlyI GAGTC 1 cut(s) 296
MseI TTAA 3 cut(s) 45, 543, 670
MslI CAYNNNNRTG 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 2 cut(s) 243, 438
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 4 cut(s) 279, 354, 478, 579
NlaIII CATG 4 cut(s) 355, 430, 457, 586
NlaIV GGNNCC 3 cut(s) 69, 107, 225
NmuCI GTSAC 3 cut(s) 144, 209, 377
PagI TCATGA 1 cut(s) 582
PceI AGGCCT 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 2 cut(s) 438, 527
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 321
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp124BI GAGCTC 3 cut(s) 237, 297, 345
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 3 cut(s) 69, 107, 225
PspPI GGNCC 2 cut(s) 67, 223
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 3 cut(s) 237, 297, 345
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 3 cut(s) 45, 543, 670
Sau3AI GATC 4 cut(s) 279, 354, 478, 579
Sau96I GGNCC 2 cut(s) 67, 223
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 3 cut(s) 237, 297, 345
SetI ASST 9 cut(s) 163, 216, 237, 297, 345, 372, 474, 521, 591
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 2 cut(s) 67, 223
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 1 cut(s) 626
SseBI AGGCCT 1 cut(s) 85
SsiI CCGC 3 cut(s) 201, 262, 277
SstI GAGCTC 3 cut(s) 237, 297, 345
StuI AGGCCT 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 3 cut(s) 144, 377, 505
TaiI ACGT 3 cut(s) 216, 372, 474
TaqI TCGA 2 cut(s) 357, 507
TasI AATT 1 cut(s) 626
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 3 cut(s) 45, 543, 670
Tru9I TTAA 3 cut(s) 45, 543, 670
TseFI GTSAC 3 cut(s) 144, 209, 377
Tsp45I GTSAC 3 cut(s) 144, 209, 377
TspDTI ATGAA 4 cut(s) 470, 487, 599, 656
TspGWI ACGGA 3 cut(s) 153, 501, 615
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 67, 223
XapI RAATTY 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.