MD13G1272800.v1.1

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013958
Physical Location & Seq
Forward (+)
36777821 .. 36778273
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1272800.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGACCCCGACGAGCTTAGACGCGTGTTCCAAATGTTTGATCGCAATGGGGACGGGCGGATCACCAAGCATGAGCTCAATGACTCCTTGGAGAACTTGGGAATCTTCATCGCGGACAAGGAGCTCTTCAACATGATCAAGAAAATCGATGTGAATGGAGACGGGTGTGTGGACATTGATGAGTTCGGGGAGTTGTATCAGTCTATCATGGACGAGCGTGATGAGGAGGAGGATATGAAGGAGGCATTCAATGTGTTTGATCAAAATGGAGACGGGTTCATCACTGTGGATGAGTTGAGGTCGGTTTTGTCGTCCCTCGGGCTTAAGCAAGGGAGGACAATAGAGGAGTGCAAAAGGATGATCATGAAGGTGGATATGGATGGAGATGGAAGGGTCAACTACAAGGAGTTCAAGCAAATGTTGAAGGGGGGTGGGTTTAGCGCATTGAGTTAA

Protein Analysis

151

Amino Acids

17.19

Weight (kDa)

4.45

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 4 - 67 8e-14 EF-hand domain pair
EF-hand_1 PF00036 5 - 28 1.1e-06 EF hand domain
EF-hand_6 PF13405 5 - 34 8.9e-08 EF-hand domain
EF-hand_5 PF13202 6 - 27 3.5e-07 EF hand
EF-hand_1 PF00036 41 - 67 4e-06 EF hand domain
AIF-1 PF21008 67 - 144 4.1e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 76 - 141 6.5e-16 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 1.1e-09 EF-hand domain
EF-hand_1 PF00036 79 - 106 1.1e-08 EF hand domain
EF-hand_5 PF13202 80 - 103 7.9e-08 EF hand
EF-hand_8 PF13833 92 - 142 4.1e-07 EF-hand domain pair
EF-hand_1 PF00036 116 - 142 7.2e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 24, 113
AciI CCGC 2 cut(s) 58, 113
AclWI GGATC 1 cut(s) 68
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 4 cut(s) 130, 250, 412, 424
AluBI AGCT 3 cut(s) 16, 76, 124
AluI AGCT 3 cut(s) 16, 76, 124
Alw21I GWGCWC 2 cut(s) 78, 126
Alw26I GTCTC 2 cut(s) 153, 264
AlwI GGATC 1 cut(s) 68
Ama87I CYCGRG 1 cut(s) 317
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 78, 126
Bbv12I GWGCWC 2 cut(s) 78, 126
BccI CCATC 2 cut(s) 374, 380
BclI TGATCA 3 cut(s) 135, 259, 360
BcoDI GTCTC 2 cut(s) 153, 264
BfrI CTTAAG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 87, 316
Bse3DI GCAATG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 87, 316
BseGI GGATG 3 cut(s) 295, 363, 385
BseMI GCAATG 1 cut(s) 52
BseRI GAGGAG 3 cut(s) 239, 242, 359
Bsh1236I CGCG 2 cut(s) 24, 113
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 78, 126
BsiHKCI CYCGRG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 65, 298
BsmAI GTCTC 2 cut(s) 153, 264
BsmBI CGTCTC 2 cut(s) 153, 264
BsmFI GGGAC 2 cut(s) 65, 298
BsmI GAATGC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 78, 126
Bsp143I GATC 5 cut(s) 40, 60, 135, 259, 360
BspACI CCGC 2 cut(s) 58, 113
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 2 cut(s) 24, 113
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 87, 316
BssMI GATC 5 cut(s) 40, 60, 135, 259, 360
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 131
BstAFI CTTAAG 1 cut(s) 323
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 295, 363, 385
BstFNI CGCG 2 cut(s) 24, 113
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 5 cut(s) 43, 63, 138, 262, 363
BstMAI GTCTC 2 cut(s) 153, 264
BstMBI GATC 5 cut(s) 40, 60, 135, 259, 360
BstUI CGCG 2 cut(s) 24, 113
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 3 cut(s) 295, 363, 385
BtsIMutI CAGTG 1 cut(s) 282
CciI TCATGA 1 cut(s) 363
CfoI GCGC 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 30
CviAII CATG 4 cut(s) 71, 133, 208, 364
CviJI RGCY 4 cut(s) 16, 76, 124, 322
CviKI_1 RGCY 4 cut(s) 16, 76, 124, 322
DdeI CTNAG 1 cut(s) 17
DpnI GATC 5 cut(s) 42, 62, 137, 261, 362
DpnII GATC 5 cut(s) 40, 60, 135, 259, 360
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 2 cut(s) 76, 124
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 78, 126
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 2 cut(s) 76, 124
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 2 cut(s) 76, 124
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 2 cut(s) 78, 126
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 2 cut(s) 153, 264
FaeI CATG 4 cut(s) 74, 136, 211, 367
FaiI YATR 6 cut(s) 72, 134, 209, 236, 365, 377
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 2 cut(s) 65, 298
FatI CATG 4 cut(s) 70, 132, 207, 363
FbaI TGATCA 3 cut(s) 135, 259, 360
FokI GGATG 3 cut(s) 302, 370, 392
FriOI GRGCYC 2 cut(s) 78, 126
GlaI GCGC 1 cut(s) 442
HgaI GACGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 443
Hin1II CATG 4 cut(s) 74, 136, 211, 367
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 83, 102
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 172, 397
Hpy188III TCNNGA 2 cut(s) 139, 364
Hpy8I GTNNAC 2 cut(s) 172, 397
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 4 cut(s) 232, 361, 384, 418
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 1 cut(s) 351
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 4 cut(s) 74, 136, 211, 367
HspAI GCGC 1 cut(s) 441
Ksp22I TGATCA 3 cut(s) 135, 259, 360
Kzo9I GATC 5 cut(s) 40, 60, 135, 259, 360
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 121
MalI GATC 5 cut(s) 42, 62, 137, 261, 362
MboI GATC 5 cut(s) 40, 60, 135, 259, 360
MboII GAAGA 2 cut(s) 97, 118
MhlI GDGCHC 2 cut(s) 78, 126
MluI ACGCGT 1 cut(s) 22
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 8 cut(s) 217, 220, 223, 235, 291, 326, 327, 337
MseI TTAA 2 cut(s) 324, 451
MslI CAYNNNNRTG 2 cut(s) 284, 368
MspCI CTTAAG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 245
MvnI CGCG 2 cut(s) 24, 113
NdeII GATC 5 cut(s) 40, 60, 135, 259, 360
NlaIII CATG 4 cut(s) 74, 136, 211, 367
NlaIV GGNNCC 1 cut(s) 6
PagI TCATGA 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PctI GAATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 102
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 2 cut(s) 78, 126
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 284, 368
SacI GAGCTC 2 cut(s) 78, 126
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 324, 451
Sau3AI GATC 5 cut(s) 40, 60, 135, 259, 360
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 2 cut(s) 78, 126
SetI ASST 5 cut(s) 18, 78, 126, 302, 372
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 284, 368
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SsiI CCGC 2 cut(s) 58, 113
SstI GAGCTC 2 cut(s) 78, 126
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 147
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 2 cut(s) 324, 451
Tru9I TTAA 2 cut(s) 324, 451
TscAI CASTG 1 cut(s) 289
TspDTI ATGAA 4 cut(s) 97, 251, 268, 380
TspRI CASTG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 4
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.