AT2G44060

Water Stress and Hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008529
Physical Location & Seq
Forward (+)
18226263 .. 18228293
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44060.1

Sequence Viewer

Length: 978 bp
ATGTCGACATCTGAGGATAAACCGGAAATCATCAGTAGAGTAGTTCATCAGGAGGGTGACGTGGAAATCGTCGATAGAAGTCAGAAGGATAAGGACGAGGAAAAAGAAGAAGGGAAAGGTGGATTCCTCGATAAGGTGAAAGATTTCATTCATGACATTGGTGAGAAACTCGAGGGAACCATTGGCTTTGGGAAGCCAACTGCTGATGTCTCTGCGATTCATATCCCTAAGATCAATCTTGAGAGGGCAGATATTGTTGTGGATGTGCTTGTCAAGAACCCGAATCCAGTTCCTATCCCTCTCATCGATGTCAACTACCTGGTCGAGAGCGATGGGAGGAAACTGGTTTCTGGTTTGATCCCGGATGCTGGAACACTCAAGGCTCATGGAGAAGAAACTGTGAAGATACCATTGACGTTGATCTATGATGACATCAAGAGCACTTACAACGATATCAACCCCGGGATGATCATACCTTACAGAATCAAGGTTGATCTGATTGTGGATGTGCCAGTATTGGGAAGACTGACATTGCCGCTGGAGAAATGTGGAGAGATCCCAATTCCAAAGAAACCTGATGTTGATATCGAGAAGATTAAGTTCCAGAAGTTCTCTTTGGAGGAAACCGTGGCGATTCTCCATGTGAGGCTTCAGAACATGAATGATTTCGACTTGGGGCTCAATGACTTGGACTGTGAAGTTTGGCTGTGTGATGTAAGCATTGGGAAAGCAGAGATCGCGGACTCGATCAAGCTTGACAAAAACGGAAGCGGATTGATTAATGTGCCGATGACATTCCGACCAAAGGACTTTGGTTCTGCGCTTTGGGATATGATTCGTGGTAAAGGAACAGGGTACACAATCAAAGGTAATATTGATGTTGATACACCATTTGGAGCTATGAAGCTTCCTATTATCAAGGAAGGTGGAGAGACCCGTCTGAAGAAGGAAGATGATGATGACGACGATGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

325

Amino Acids

36.04

Weight (kDa)

4.69

Isoelectric Point (pI)

20.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 89 - 182 2.3e-18 Late embryogenesis abundant protein
LEA_2 PF03168 214 - 309 6.1e-18 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 740
AciI CCGC 3 cut(s) 536, 740, 771
AclWI GGATC 2 cut(s) 352, 550
AcuI CTGAAG 2 cut(s) 635, 962
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 4 cut(s) 133, 368, 518, 805
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 318
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AluBI AGCT 3 cut(s) 754, 899, 907
AluI AGCT 3 cut(s) 754, 899, 907
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 2 cut(s) 214, 926
AlwI GGATC 2 cut(s) 352, 550
Ama87I CYCGRG 2 cut(s) 170, 461
AseI ATTAAT 1 cut(s) 780
Asp700I GAANNNNTTC 2 cut(s) 143, 665
AspLEI GCGC 1 cut(s) 823
AsuC2I CCSGG 3 cut(s) 362, 462, 463
AsuHPI GGTGA 3 cut(s) 68, 148, 173
AvaI CYCGRG 2 cut(s) 170, 461
BanII GRGCYC 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 443
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 468
BcnI CCSGG 3 cut(s) 362, 462, 463
BcoDI GTCTC 2 cut(s) 214, 926
BisI GCNGC 1 cut(s) 536
BlsI GCNGC 1 cut(s) 537
Bme1390I CCNGG 4 cut(s) 320, 362, 462, 463
BmeT110I CYCGRG 2 cut(s) 170, 461
BmgBI CACGTC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 4 cut(s) 320, 362, 462, 463
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 560
BpuEI CTTGAG 2 cut(s) 260, 362
BpuMI CCSGG 3 cut(s) 362, 462, 463
Bsa29I ATCGAT 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 926
BsaJI CCNNGG 3 cut(s) 460, 461, 627
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 368, 518, 805
Bse1I ACTGG 3 cut(s) 287, 348, 512
Bse3DI GCAATG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 221
BseBI CCWGG 1 cut(s) 320
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 460, 461, 627
BseGI GGATG 4 cut(s) 268, 370, 471, 511
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 4 cut(s) 133, 368, 518, 805
BseMI GCAATG 1 cut(s) 530
BseNI ACTGG 3 cut(s) 287, 348, 512
Bsh1236I CGCG 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 2 cut(s) 170, 461
BsiSI CCGG 3 cut(s) 23, 362, 462
BslI CCNNNNNNNGG 4 cut(s) 133, 368, 518, 805
BsmAI GTCTC 2 cut(s) 214, 926
Bso31I GGTCTC 1 cut(s) 926
BsoBI CYCGRG 2 cut(s) 170, 461
Bsp1286I GDGCHC 2 cut(s) 443, 681
Bsp143I GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
BspACI CCGC 3 cut(s) 536, 740, 771
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 1 cut(s) 306
BspFNI CGCG 1 cut(s) 740
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 2 cut(s) 352, 550
BspTNI GGTCTC 1 cut(s) 926
BsrDI GCAATG 1 cut(s) 530
BsrI ACTGG 3 cut(s) 287, 348, 512
BssECI CCNNGG 3 cut(s) 460, 461, 627
BssMI GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 400, 628, 695
BstDEI CTNAG 2 cut(s) 12, 228
BstDSI CCRYGG 1 cut(s) 627
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 4 cut(s) 268, 370, 471, 511
BstFNI CGCG 1 cut(s) 740
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 8 cut(s) 234, 360, 423, 471, 496, 558, 738, 750
BstMAI GTCTC 2 cut(s) 214, 926
BstMBI GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
BstMWI GCNNNNNNNGC 1 cut(s) 737
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 4 cut(s) 318, 360, 460, 461
BstUI CGCG 1 cut(s) 740
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 555
BstYI RGATCY 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 306
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 4 cut(s) 268, 370, 471, 511
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 823
Cfr9I CCCGGG 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 306
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 1 cut(s) 856
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 4 cut(s) 152, 386, 641, 658
CviJI RGCY 9 cut(s) 186, 196, 383, 649, 679, 706, 754, 899, 907
CviKI_1 RGCY 9 cut(s) 186, 196, 383, 649, 679, 706, 754, 899, 907
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 2 cut(s) 12, 228
DpnI GATC 8 cut(s) 233, 359, 422, 470, 495, 557, 737, 749
DpnII GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 1 cut(s) 926
Eco32I GATATC 2 cut(s) 454, 586
Eco57I CTGAAG 2 cut(s) 635, 962
Eco88I CYCGRG 2 cut(s) 170, 461
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 2 cut(s) 454, 586
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 4 cut(s) 155, 389, 644, 661
FaiI YATR 9 cut(s) 153, 222, 387, 426, 473, 642, 659, 833, 902
FatI CATG 4 cut(s) 151, 385, 640, 657
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 536
FokI GGATG 4 cut(s) 275, 377, 478, 518
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 1 cut(s) 536
GlaI GCGC 1 cut(s) 822
GluI GCNGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 560
HapII CCGG 3 cut(s) 23, 362, 462
HhaI GCGC 1 cut(s) 823
Hin1II CATG 4 cut(s) 155, 389, 644, 661
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 2 cut(s) 6, 313
HindII GTYRAC 2 cut(s) 6, 313
HindIII AAGCTT 2 cut(s) 752, 905
HinfI GANTC 7 cut(s) 123, 217, 283, 483, 634, 743, 835
HpaII CCGG 3 cut(s) 23, 362, 462
HphI GGTGA 3 cut(s) 68, 148, 173
Hpy166II GTNNAC 3 cut(s) 6, 313, 858
Hpy188I TCNGA 6 cut(s) 13, 84, 498, 654, 800, 942
Hpy188III TCNNGA 8 cut(s) 50, 152, 239, 274, 325, 436, 589, 604
Hpy8I GTNNAC 3 cut(s) 6, 313, 858
Hpy99I CGWCG 2 cut(s) 74, 968
HpyAV CCTTC 4 cut(s) 79, 104, 917, 940
HpyCH4III ACNGT 3 cut(s) 400, 628, 695
HpyCH4IV ACGT 2 cut(s) 60, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 737
HpyF3I CTNAG 2 cut(s) 12, 228
HpySE526I ACGT 2 cut(s) 60, 416
Hsp92II CATG 4 cut(s) 155, 389, 644, 661
HspAI GCGC 1 cut(s) 821
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
LmnI GCTCC 1 cut(s) 896
LweI GCATC 1 cut(s) 355
MabI ACCWGGT 1 cut(s) 318
MaeII ACGT 2 cut(s) 60, 416
MaeIII GTNAC 1 cut(s) 56
MalI GATC 8 cut(s) 233, 359, 422, 470, 495, 557, 737, 749
MboI GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
MboII GAAGA 7 cut(s) 119, 404, 415, 534, 604, 955, 962
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 2 cut(s) 443, 681
MluCI AATT 1 cut(s) 561
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 823
MroXI GAANNNNTTC 2 cut(s) 143, 665
MseI TTAA 2 cut(s) 597, 780
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 3 cut(s) 23, 362, 462
MspR9I CCNGG 4 cut(s) 320, 362, 462, 463
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 740
MwoI GCNNNNNNNGC 1 cut(s) 737
NciI CCSGG 3 cut(s) 362, 462, 463
NdeII GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
NlaIII CATG 4 cut(s) 155, 389, 644, 661
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 56
PaeR7I CTCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 143, 665
PfeI GAWTC 6 cut(s) 123, 217, 283, 483, 634, 835
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 1 cut(s) 537
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
PshBI ATTAAT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 178
PspXI VCTCGAGB 1 cut(s) 170
PsuI RGATCY 1 cut(s) 555
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 597, 780
SatI GCNGC 1 cut(s) 536
Sau3AI GATC 8 cut(s) 231, 357, 420, 468, 493, 555, 735, 747
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 4 cut(s) 320, 362, 462, 463
SduI GDGCHC 2 cut(s) 443, 681
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 170
SmaI CCCGGG 1 cut(s) 463
SmlI CTYRAG 3 cut(s) 170, 239, 377
SmoI CTYRAG 3 cut(s) 170, 239, 377
Sse9I AATT 1 cut(s) 561
SsiI CCGC 3 cut(s) 536, 740, 771
SspI AATATT 1 cut(s) 874
StyD4I CCNGG 4 cut(s) 318, 360, 460, 461
TaaI ACNGT 3 cut(s) 400, 628, 695
TaiI ACGT 2 cut(s) 63, 419
TaqI TCGA 9 cut(s) 5, 72, 129, 171, 306, 324, 588, 669, 746
TasI AATT 1 cut(s) 561
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 6 cut(s) 123, 217, 283, 483, 634, 835
Tru1I TTAA 2 cut(s) 597, 780
Tru9I TTAA 2 cut(s) 597, 780
TseFI GTSAC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 6 cut(s) 35, 136, 140, 209, 674, 917
TspGWI ACGGA 1 cut(s) 780
TspMI CCCGGG 1 cut(s) 461
VspI ATTAAT 1 cut(s) 780
XagI CCTNNNNNAGG 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 170
XmaI CCCGGG 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 143, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.