RchiOBHm_Chr2g0084381

abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008529
Physical Location & Seq
Reverse (-)
183886 .. 186448
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45997

Sequence Viewer

Length: 969 bp
ATGGCATCCTATGATGACTCAGAAAGATCAGATCGCGGCATTAAGGAGGAGGAGAAAAAAGAGCGGAAACACAATGGGGAGGAGGAGGAGGGTGACAGCTTCATAGATAAGGTGAAGGATTTCATTCATGACATTGGCGAGAAGATCGAGGGAGCTATCGGATTTGGTAAGCCGACTGCAGATGTGGCTGCAATTCACATCCCTTGCATAAATCTGGAGAAGGCAGAGATTGTGGTTGATGTGCTTATTAAGAACCCAAATCCGGTGCCCATCCCGCTCATTGACATAAACTACTTGATTGAAAGTGATGGTAGGAAACTGGTGTCCGGATTGATCCCGGACGCTGGAACAATCCAGGCCCATGGAGAAGAGACTGTCAAAGTTCCACTTCACTTGATTTACGATGACATCAAGAGCACCTACAATGATATCGAGCCTGGAAGCATCATTCCCTACAGGATCAAGGTTGATCTCATTGTGGATGTCCCTGTTCTTGGCAGGCTCACTCTCCCACTTGAGAAAACTGGAGAGATCCCCATCCCCTACAAACCTGATGTTGATGTTGACAAAATCAAGTTTCAGAAATTCTCCTTTGAAGAAACTGTTGCAGTACTCCATTTGAAGCTCGAGAACATGAATGACTTTGACTTGGGCCTCAATGCTTTAGACTACGAGATTTGGCTCTGTGAGGTCAGCATTGGAGGCGCTGAGCTCTCCAAATCTGCCAACCTTGAAAAGAAAGGTATCACTTTTGTTGACGTTCCTATTACCTTCAGGCCAAAGGATTTTGGCTCTGCGCTCTGGGATATGATCCGGGGTAGGGGTACTGGTTATACCTTCAAAGGCCATATCGATGTTGATACTCCCTTTGGGGCCATGAAGTTACCCATTGTCAAGGAGGGTGGTACTACACGCCTCAAGAAGAGCAAGGAAGATGGTGGCGACGATGATGACGATGATGAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

322

Amino Acids

35.77

Weight (kDa)

4.67

Isoelectric Point (pI)

24.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 81 - 176 2.2e-18 Late embryogenesis abundant protein
LEA_2 PF03168 206 - 301 9.5e-17 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 2 cut(s) 64, 277
AccII CGCG 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 326
AciI CCGC 3 cut(s) 36, 64, 275
AclWI GGATC 4 cut(s) 328, 467, 526, 805
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 757
AfaI GTAC 3 cut(s) 612, 826, 907
AfiI CCNNNNNNNGG 4 cut(s) 262, 344, 494, 820
AgsI TTSAA 5 cut(s) 302, 596, 622, 734, 841
AjnI CCWGG 2 cut(s) 354, 436
AluBI AGCT 4 cut(s) 99, 155, 625, 712
AluI AGCT 4 cut(s) 99, 155, 625, 712
Alw21I GWGCWC 2 cut(s) 419, 714
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 4 cut(s) 328, 467, 526, 805
Ama87I CYCGRG 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 326
AoxI GGCC 5 cut(s) 357, 652, 776, 844, 873
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 584
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 2 cut(s) 707, 799
AspS9I GGNCC 3 cut(s) 358, 652, 873
AsuC2I CCSGG 2 cut(s) 338, 815
AsuHPI GGTGA 2 cut(s) 104, 124
AvaI CYCGRG 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 270
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 714
Bbv12I GWGCWC 2 cut(s) 419, 714
BbvI GCAGC 1 cut(s) 175
BccI CCATC 4 cut(s) 278, 302, 545, 929
BciT130I CCWGG 2 cut(s) 356, 438
BcnI CCSGG 2 cut(s) 338, 815
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 967
BfmI CTRYAG 2 cut(s) 177, 454
BfoI RGCGCY 1 cut(s) 708
BisI GCNGC 2 cut(s) 37, 189
BlpI GCTNAGC 1 cut(s) 708
BlsI GCNGC 2 cut(s) 38, 190
BmcAI AGTACT 1 cut(s) 612
Bme1390I CCNGG 4 cut(s) 338, 356, 438, 815
BmeT110I CYCGRG 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 358, 652, 873
BmiI GGNNCC 2 cut(s) 267, 874
BmrFI CCNGG 4 cut(s) 338, 356, 438, 815
BmsI GCATC 2 cut(s) 14, 453
BpmI CTGGAG 2 cut(s) 236, 546
Bpu1102I GCTNAGC 1 cut(s) 708
BpuEI CTTGAG 2 cut(s) 536, 902
BpuMI CCSGG 2 cut(s) 338, 815
Bsa29I ATCGAT 1 cut(s) 852
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 2 cut(s) 361, 814
BsaWI WCCGGW 2 cut(s) 262, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 344, 494, 820
Bse1I ACTGG 3 cut(s) 324, 529, 832
Bse8I GATNNNNATC 1 cut(s) 536
BseAI TCCGGA 1 cut(s) 326
BseBI CCWGG 2 cut(s) 356, 438
BseCI ATCGAT 1 cut(s) 852
BseDI CCNNGG 2 cut(s) 361, 814
BseGI GGATG 5 cut(s) 5, 198, 270, 487, 537
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 4 cut(s) 262, 344, 494, 820
BseMII CTCAG 2 cut(s) 33, 699
BseNI ACTGG 3 cut(s) 324, 529, 832
BseRI GAGGAG 5 cut(s) 62, 65, 95, 98, 101
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 5 cut(s) 359, 654, 778, 846, 875
BshNI GGYRCC 1 cut(s) 265
BshVI ATCGAT 1 cut(s) 852
BsiHKAI GWGCWC 2 cut(s) 419, 714
BsiHKCI CYCGRG 1 cut(s) 626
BsiSI CCGG 4 cut(s) 263, 327, 338, 814
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 4 cut(s) 262, 344, 494, 820
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 5 cut(s) 359, 654, 778, 846, 875
BsoBI CYCGRG 1 cut(s) 626
Bsp1286I GDGCHC 3 cut(s) 270, 419, 714
Bsp13I TCCGGA 1 cut(s) 326
Bsp143I GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
Bsp1720I GCTNAGC 1 cut(s) 708
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 3 cut(s) 36, 64, 275
BspANI GGCC 5 cut(s) 359, 654, 778, 846, 875
BspCNI CTCAG 2 cut(s) 32, 700
BspDI ATCGAT 1 cut(s) 852
BspEI TCCGGA 1 cut(s) 326
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 267, 874
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 4 cut(s) 328, 467, 526, 805
BspQI GCTCTTC 1 cut(s) 917
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 2 cut(s) 64, 277
BsrI ACTGG 3 cut(s) 324, 529, 832
BssECI CCNNGG 2 cut(s) 361, 814
BssMI GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 2 cut(s) 356, 438
Bst4CI ACNGT 2 cut(s) 376, 604
Bst6I CTCTTC 2 cut(s) 363, 917
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 19, 708
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 5 cut(s) 5, 198, 270, 487, 537
BstFNI CGCG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 708
BstHHI GCGC 2 cut(s) 707, 799
BstKTI GATC 8 cut(s) 29, 34, 147, 336, 462, 472, 534, 813
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
BstMWI GCNNNNNNNGC 3 cut(s) 185, 274, 702
BstNI CCWGG 2 cut(s) 356, 438
BstSCI CCNGG 4 cut(s) 336, 354, 436, 813
BstSFI CTRYAG 2 cut(s) 177, 454
BstSLI GKGCMC 1 cut(s) 270
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 531
Bsu15I ATCGAT 1 cut(s) 852
BsuRI GGCC 5 cut(s) 359, 654, 778, 846, 875
BsuTUI ATCGAT 1 cut(s) 852
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 5 cut(s) 5, 198, 270, 487, 537
Cac8I GCNNGC 1 cut(s) 500
CciI TCATGA 1 cut(s) 127
CfoI GCGC 2 cut(s) 707, 799
Cfr13I GGNCC 3 cut(s) 358, 652, 873
ClaI ATCGAT 1 cut(s) 852
CseI GACGC 1 cut(s) 350
Csp6I GTAC 3 cut(s) 611, 825, 906
CspCI CAANNNNNGTGG 4 cut(s) 375, 410, 883, 918
CviAII CATG 4 cut(s) 128, 362, 634, 877
CviQI GTAC 3 cut(s) 611, 825, 906
DdeI CTNAG 2 cut(s) 19, 708
DpnI GATC 8 cut(s) 28, 33, 146, 335, 461, 471, 533, 812
DpnII GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
Eam1104I CTCTTC 2 cut(s) 363, 917
EarI CTCTTC 2 cut(s) 363, 917
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 430
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 757
Eco88I CYCGRG 1 cut(s) 626
EcoICRI GAGCTC 1 cut(s) 712
EcoRII CCWGG 2 cut(s) 354, 436
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 4 cut(s) 131, 365, 637, 880
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 470
FatI CATG 4 cut(s) 127, 361, 633, 876
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 37, 189
FokI GGATG 4 cut(s) 185, 257, 494, 524
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 2 cut(s) 37, 189
FspBI CTAG 1 cut(s) 967
GlaI GCGC 2 cut(s) 706, 798
GluI GCNGC 2 cut(s) 37, 189
GsuI CTGGAG 2 cut(s) 236, 546
HaeII RGCGCY 1 cut(s) 708
HaeIII GGCC 5 cut(s) 359, 654, 778, 846, 875
HapII CCGG 4 cut(s) 263, 327, 338, 814
HgaI GACGC 1 cut(s) 350
HhaI GCGC 2 cut(s) 707, 799
Hin1II CATG 4 cut(s) 131, 365, 637, 880
Hin6I GCGC 2 cut(s) 705, 797
HinP1I GCGC 2 cut(s) 705, 797
HincII GTYRAC 2 cut(s) 565, 757
HindII GTYRAC 2 cut(s) 565, 757
HinfI GANTC 1 cut(s) 17
HpaII CCGG 4 cut(s) 263, 327, 338, 814
HphI GGTGA 2 cut(s) 104, 124
Hpy166II GTNNAC 2 cut(s) 565, 757
Hpy188I TCNGA 4 cut(s) 22, 31, 161, 582
Hpy188III TCNNGA 6 cut(s) 128, 215, 327, 412, 628, 919
Hpy8I GTNNAC 2 cut(s) 565, 757
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 4 cut(s) 109, 214, 781, 847
HpyCH4III ACNGT 2 cut(s) 376, 604
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 4 cut(s) 179, 191, 207, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 274, 702
HpyF3I CTNAG 2 cut(s) 19, 708
HpySE526I ACGT 1 cut(s) 759
Hsp92II CATG 4 cut(s) 131, 365, 637, 880
HspAI GCGC 2 cut(s) 705, 797
Kpn2I TCCGGA 1 cut(s) 326
Kzo9I GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
LguI GCTCTTC 1 cut(s) 917
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 14, 453
MaeI CTAG 1 cut(s) 967
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 2 cut(s) 92, 882
MalI GATC 8 cut(s) 28, 33, 146, 335, 461, 471, 533, 812
MbiI CCGCTC 2 cut(s) 64, 277
MboI GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
MboII GAAGA 5 cut(s) 154, 380, 608, 934, 944
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 3 cut(s) 270, 419, 714
MluCI AATT 2 cut(s) 192, 584
MlyI GAGTC 1 cut(s) 11
MroI TCCGGA 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 2 cut(s) 42, 249
MslI CAYNNNNRTG 1 cut(s) 852
MspI CCGG 4 cut(s) 263, 327, 338, 814
MspR9I CCNGG 4 cut(s) 338, 356, 438, 815
MvaI CCWGG 2 cut(s) 356, 438
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 3 cut(s) 185, 274, 702
NciI CCSGG 2 cut(s) 338, 815
NcoI CCATGG 1 cut(s) 361
NdeII GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
NlaIII CATG 4 cut(s) 131, 365, 637, 880
NlaIV GGNNCC 2 cut(s) 267, 874
NmuCI GTSAC 1 cut(s) 92
PaeR7I CTCGAG 1 cut(s) 626
PagI TCATGA 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 917
PcsI WCGNNNNNNNCGW 1 cut(s) 951
PdmI GAANNNNTTC 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 336
PkrI GCNGC 2 cut(s) 38, 190
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 354, 436
PspGI CCWGG 2 cut(s) 354, 436
PspN4I GGNNCC 2 cut(s) 267, 874
PspPI GGNCC 3 cut(s) 358, 652, 873
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 531
RsaI GTAC 3 cut(s) 612, 826, 907
RsaNI GTAC 3 cut(s) 611, 825, 906
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 917
SaqAI TTAA 2 cut(s) 42, 249
SatI GCNGC 2 cut(s) 37, 189
Sau3AI GATC 8 cut(s) 26, 31, 144, 333, 459, 469, 531, 810
Sau96I GGNCC 3 cut(s) 358, 652, 873
ScaI AGTACT 1 cut(s) 612
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 4 cut(s) 338, 356, 438, 815
SduI GDGCHC 3 cut(s) 270, 419, 714
SfaNI GCATC 2 cut(s) 14, 453
SfcI CTRYAG 2 cut(s) 177, 454
Sfr274I CTCGAG 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 3 cut(s) 515, 626, 917
SmoI CTYRAG 3 cut(s) 515, 626, 917
Sse9I AATT 2 cut(s) 192, 584
SsiI CCGC 3 cut(s) 36, 64, 275
SspMI CTAG 1 cut(s) 967
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 4 cut(s) 336, 354, 436, 813
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 2 cut(s) 376, 604
TaiI ACGT 1 cut(s) 762
TaqI TCGA 4 cut(s) 147, 432, 627, 852
TasI AATT 2 cut(s) 192, 584
TatI WGTACW 1 cut(s) 610
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 2 cut(s) 42, 249
Tru9I TTAA 2 cut(s) 42, 249
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 5 cut(s) 91, 112, 116, 650, 893
XapI RAATTY 1 cut(s) 584
XhoI CTCGAG 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 967
ZrmI AGTACT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.