pycom290g00100

abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0008529
Physical Location & Seq
Reverse (-)
101940 .. 102799
860 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom290g00100.1

Sequence Viewer

Length: 426 bp
ATGAATGACTTTGACTTAGGCCTCAATGCTCTCGACTACGAGATTTGGCTCTGTGATGTCCGCATTGGAGGTGCAGAGCTCTCCAAGTCTGCCAATCTTGCTAAAAGGGGCATCACTTATGTTGACGTTCCCATTACCTTCAGGCCTAAGGATTTTGGGTCAGCGCTCTGGGATATGATTCGGGGAATGGGTACCGGCTACACCATCAAAGGGAATATCAATGTGGACACGCCCTTTGGAGCCATGAAGTTGCCGATCGTCAAGGAGGGTTGTACTACACGCCTCAAGAAGAACAAGGAAGATGGTGGTGATGACGACGATGATGAGGAGTGGAGAGGGATGTTGGGTTGGCAAGTGAAACTAGTAGTTGACGTGGTCTGTTTATCTGTACTGGTGCTTGAGAATAATAGACAAACAGGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

142

Amino Acids

15.65

Weight (kDa)

4.74

Isoelectric Point (pI)

21.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 2 - 90 7.1e-16 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 191
AciI CCGC 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 124
AfaI GTAC 3 cut(s) 193, 274, 390
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 210
AhlI ACTAGT 1 cut(s) 361
AjiI CACGTC 1 cut(s) 373
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw21I GWGCWC 1 cut(s) 81
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 2 cut(s) 19, 143
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
Asp718I GGTACC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 320
AxyI CCTNAGG 1 cut(s) 147
BanI GGYRCC 1 cut(s) 191
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BccI CCATC 2 cut(s) 212, 296
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 1 cut(s) 362
BfoI RGCGCY 1 cut(s) 167
BmgBI CACGTC 1 cut(s) 373
BmiI GGNNCC 2 cut(s) 193, 241
BmsI GCATC 1 cut(s) 120
BpuEI CTTGAG 2 cut(s) 269, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 2 cut(s) 21, 145
BshNI GGYRCC 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 1 cut(s) 210
BsnI GGCC 2 cut(s) 21, 145
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 1 cut(s) 61
BspANI GGCC 2 cut(s) 21, 145
BspLI GGNNCC 2 cut(s) 193, 241
BspT107I GGYRCC 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 194
BssMI GATC 1 cut(s) 255
BstDEI CTNAG 2 cut(s) 16, 147
BstF5I GGATG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 1 cut(s) 258
BstMBI GATC 1 cut(s) 255
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 147
BsuRI GGCC 2 cut(s) 21, 145
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 345
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 194
Csp6I GTAC 3 cut(s) 192, 273, 389
CviAII CATG 1 cut(s) 244
CviJI RGCY 6 cut(s) 21, 49, 79, 145, 198, 242
CviKI_1 RGCY 6 cut(s) 21, 49, 79, 145, 198, 242
CviQI GTAC 3 cut(s) 192, 273, 389
DdeI CTNAG 2 cut(s) 16, 147
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
Ecl136II GAGCTC 1 cut(s) 79
Eco147I AGGCCT 2 cut(s) 21, 145
Eco24I GRGCYC 1 cut(s) 81
Eco47III AGCGCT 1 cut(s) 165
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 124
Eco81I CCTNAGG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 1 cut(s) 247
FaiI YATR 3 cut(s) 120, 176, 245
FatI CATG 1 cut(s) 243
FokI GGATG 1 cut(s) 352
FriOI GRGCYC 1 cut(s) 81
FspBI CTAG 1 cut(s) 362
GlaI GCGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 2 cut(s) 21, 145
HapII CCGG 1 cut(s) 195
HhaI GCGC 1 cut(s) 166
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 2 cut(s) 124, 370
HindII GTYRAC 2 cut(s) 124, 370
HinfI GANTC 1 cut(s) 178
HpaII CCGG 1 cut(s) 195
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 124, 226, 370
Hpy188III TCNNGA 2 cut(s) 32, 286
Hpy8I GTNNAC 3 cut(s) 124, 226, 370
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 148
HpyCH4IV ACGT 2 cut(s) 126, 372
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 16, 147
HpySE526I ACGT 2 cut(s) 126, 372
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 1 cut(s) 164
KpnI GGTACC 1 cut(s) 195
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 5 cut(s) 127, 154, 208, 377, 402
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 362
MaeII ACGT 2 cut(s) 126, 372
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 2 cut(s) 301, 311
MhlI GDGCHC 1 cut(s) 81
MnlI CCTC 6 cut(s) 32, 62, 259, 293, 319, 329
MspI CCGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 1 cut(s) 255
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 2 cut(s) 193, 241
PceI AGGCCT 2 cut(s) 21, 145
PfeI GAWTC 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 374
Ple19I CGATCG 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 193, 241
PsyI GACNNNGTC 1 cut(s) 374
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 3 cut(s) 193, 274, 390
RsaNI GTAC 3 cut(s) 192, 273, 389
SacI GAGCTC 1 cut(s) 81
Sau3AI GATC 1 cut(s) 255
SduI GDGCHC 1 cut(s) 81
SetI ASST 5 cut(s) 73, 81, 129, 140, 375
SfaNI GCATC 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 284, 398
SmoI CTYRAG 2 cut(s) 284, 398
SpeI ACTAGT 1 cut(s) 361
SseBI AGGCCT 2 cut(s) 21, 145
SsiI CCGC 1 cut(s) 61
SspMI CTAG 1 cut(s) 362
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 2 cut(s) 21, 145
TaiI ACGT 2 cut(s) 129, 375
TaqI TCGA 1 cut(s) 33
TatI WGTACW 2 cut(s) 272, 388
TfiI GAWTC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 17, 260
Tth111I GACNNNGTC 1 cut(s) 374
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.