AT2G44880

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008546
Physical Location & Seq
Forward (+)
18505101 .. 18508298
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44880.6

Sequence Viewer

Length: 1668 bp
ATGCTCCGCCACGCCATTGAGACGAACGTTCAGATCTTCACCAAATTCTTGGTTATTTCTGCTTCGGCTGTTGGAATTGGTTATGCACGTAAGTTGTTCGATCAAAGGCCTCAGAGAGACGACAGTTTCCTCAGTAACTCTATGATCAAGGCCTATCTTGAAACCCGTCAGTATCCTGATTCGTTTGCTCTTTACAGAGATCTTAGGAAGGAGACTTGTTTTGCTCCTGACAATTTTACTTTCACGACTTTGACAAAGTCATGCTCTTTGAGTATGTGTGTTTACCAAGGTCTGCAGTTGCATAGCCAGATTTGGAGATTTGGTTTTTGTGCAGATATGTATGTTTCGACTGGAGTTGTTGATATGTATGCTAAATTTGGGAAGATGGGATGTGCGAGGAATGCGTTCGATGAAATGCCTCACAGAAGTGAGGTTTCTTGGACGGCTCTAATCTCTGGGTATATTAGGTGTGGGGAGCTGGATTTGGCGAGTAAGCTCTTTGATCAGATGCCTCATGTTAAGGACGTTGTGATATATAACGCAATGATGGATGGTTTTGTTAAATCTGGTGATATGACCTCTGCTAGAAGATTGTTTGATGAGATGACTCATAAAACTGTCATCACTTGGACCACTATGATTCACGGTTACTGCAACATTAAGGACATAGACGCCGCTAGGAAGTTGTTTGATGCTATGCCAGAGAGGAACCTAGTTTCTTGGAACACAATGATTGGGGGATACTGTCAGAATAAGCAACCTCAAGAAGGTATAAGGTTGTTTCAGGAAATGCAGGCAACCACCTCGCTAGATCCGGATGATGTGACTATTTTGAGTGTGCTACCAGCTATTTCTGATACTGGTGCTCTCAGTCTCGGTGAATGGTGTCACTGTTTTGTGCAGAGGAAAAAGCTCGATAAGAAGGTGAAAGTGTGTACAGCAATTCTTGATATGTATTCGAAATGCGGGGAGATAGAGAAGGCGAAAAGAATCTTTGATGAGATGCCAGAGAAACAAGTAGCGTCTTGGAATGCTATGATACATGGGTATGCTTTGAATGGGAATGCACGTGCAGCTTTGGATCTATTCGTAACAATGATGATAGAAGAAAAGCCAGATGAGATCACAATGCTCGCGGTTATCACAGCTTGTAACCATGGTGGATTAGTGGAGGAAGGAAGAAAATGGTTCCACGTGATGAGAGAAATGGGCCTGAATGCTAAAATTGAGCACTATGGCTGTATGGTTGATCTACTAGGCAGAGCAGGGAGCCTAAAAGAAGCAGAGGATTTGATTACCAACATGCCTTTTGAGCCAAATGGTATTATTCTAAGCTCTTTCTTGTCTGCGTGTGGTCAATACAAAGATATAGAAAGGGCTGAGAGAATTCTGAAGAAGGCAGTCGAATTGGAACCTCAGAACGATGGGAATTATGTGCTGTTGAGGAACTTGTACGCTGCAGATAAAAGATGGGACGATTTCGGGATGGTGAAAAACGTGATGAGGAAGAACCAAGCCAAGAAAGAGGTTGGATGTAGCTTGATTGAGATAAATTATATCGTTTCGGAGTTTATATCAGGAGATACAACCCATCCTCATCGGCGAAGCATACATTTGGTTCTCGGAGACCTGCTTATGCACATGAATGAGGAGAAATACAACTGGTGA

Protein Analysis

555

Amino Acids

63.26

Weight (kDa)

6.33

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 184 - 209 4.3e-06 PPR repeat
PPR_2 PF13041 213 - 244 5.8e-08 PPR repeat family
PPR PF01535 216 - 243 9.4e-07 PPR repeat
PPR_2 PF13041 245 - 277 6.8e-08 PPR repeat family
PPR_1 PF12854 245 - 272 1.1e-06 PPR repeat
PPR PF01535 247 - 276 1.1e-06 PPR repeat
PPR_2 PF13041 276 - 316 3.6e-09 PPR repeat family
PPR PF01535 278 - 304 3.3e-08 PPR repeat
E_motif PF20431 493 - 555 1e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1638
AccII CGCG 1 cut(s) 1136
AccIII TCCGGA 1 cut(s) 814
AciI CCGC 4 cut(s) 7, 675, 966, 1136
AclI AACGTT 1 cut(s) 27
AclWI GGATC 2 cut(s) 806, 1089
AcsI RAATTY 3 cut(s) 44, 374, 1386
AcuI CTGAAG 1 cut(s) 1412
AcvI CACGTG 2 cut(s) 1070, 1195
AcyI GRCGYC 1 cut(s) 672
AfaI GTAC 2 cut(s) 937, 1454
AfiI CCNNNNNNNGG 1 cut(s) 767
AgsI TTSAA 2 cut(s) 161, 1057
AleI CACNNNNGTG 1 cut(s) 426
AluBI AGCT 8 cut(s) 478, 496, 848, 913, 1076, 1148, 1335, 1539
AluI AGCT 8 cut(s) 478, 496, 848, 913, 1076, 1148, 1335, 1539
Alw21I GWGCWC 2 cut(s) 868, 1233
Alw26I GTCTC 5 cut(s) 14, 111, 206, 878, 1620
AlwI GGATC 2 cut(s) 806, 1089
Aor13HI TCCGGA 1 cut(s) 814
AoxI GGCC 3 cut(s) 107, 150, 1210
ApeKI GCWGC 2 cut(s) 1073, 1457
ApoI RAATTY 3 cut(s) 44, 374, 1386
Asp700I GAANNNNTTC 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 630, 1210
AsuHPI GGTGA 5 cut(s) 31, 581, 890, 937, 1501
AsuII TTCGAA 1 cut(s) 959
AvaII GGWCC 1 cut(s) 630
BaeI ACNNNNGTAYC 2 cut(s) 1031, 1064
BbrPI CACGTG 2 cut(s) 1070, 1195
Bbv12I GWGCWC 2 cut(s) 868, 1233
BbvI GCAGC 2 cut(s) 1085, 1444
BccI CCATC 7 cut(s) 379, 541, 545, 1418, 1464, 1480, 1599
BceAI ACGGC 1 cut(s) 459
BcgI CGANNNNNNTGC 2 cut(s) 786, 820
BciVI GTATCC 2 cut(s) 183, 734
BclI TGATCA 2 cut(s) 144, 502
BcoDI GTCTC 5 cut(s) 14, 111, 206, 878, 1620
BfaI CTAG 5 cut(s) 585, 678, 713, 809, 1256
BfmI CTRYAG 2 cut(s) 293, 1458
BfuAI ACCTGC 1 cut(s) 1638
BfuI GTATCC 2 cut(s) 183, 734
BglII AGATCT 2 cut(s) 33, 199
BisI GCNGC 3 cut(s) 675, 1074, 1458
BlsI GCNGC 3 cut(s) 676, 1075, 1459
Bme18I GGWCC 1 cut(s) 630
BmgT120I GGNCC 2 cut(s) 630, 1210
BmiI GGNNCC 4 cut(s) 710, 1190, 1271, 1413
BmsI GCATC 3 cut(s) 498, 682, 993
BpmI CTGGAG 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 1 cut(s) 747
BsaAI YACGTR 3 cut(s) 89, 1070, 1195
BsaHI GRCGYC 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 1620
BsaJI CCNNGG 2 cut(s) 286, 1156
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 3 cut(s) 355, 865, 1667
Bse3DI GCAATG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 814
BseDI CCNNGG 2 cut(s) 286, 1156
BseGI GGATG 6 cut(s) 395, 556, 823, 1491, 1538, 1591
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 5 cut(s) 125, 145, 883, 1371, 1430
BseNI ACTGG 3 cut(s) 355, 865, 1667
BseRI GAGGAG 1 cut(s) 1664
BseXI GCAGC 2 cut(s) 1085, 1444
BsgI GTGCAG 3 cut(s) 351, 920, 1092
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 3 cut(s) 109, 152, 1212
BsiHKAI GWGCWC 2 cut(s) 868, 1233
BsiSI CCGG 1 cut(s) 815
BslFI GGGAC 1 cut(s) 1487
BslI CCNNNNNNNGG 1 cut(s) 767
BsmAI GTCTC 5 cut(s) 14, 111, 206, 878, 1620
BsmBI CGTCTC 2 cut(s) 14, 111
BsmFI GGGAC 1 cut(s) 1487
BsmI GAATGC 4 cut(s) 406, 1036, 1069, 1222
BsnI GGCC 3 cut(s) 109, 152, 1212
Bso31I GGTCTC 1 cut(s) 1620
Bsp119I TTCGAA 1 cut(s) 959
Bsp1286I GDGCHC 2 cut(s) 868, 1233
Bsp13I TCCGGA 1 cut(s) 814
Bsp1407I TGTACA 1 cut(s) 935
Bsp143I GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
Bsp19I CCATGG 1 cut(s) 1156
BspACI CCGC 4 cut(s) 7, 675, 966, 1136
BspANI GGCC 3 cut(s) 109, 152, 1212
BspCNI CTCAG 5 cut(s) 124, 144, 882, 1372, 1429
BspEI TCCGGA 1 cut(s) 814
BspFNI CGCG 1 cut(s) 1136
BspLI GGNNCC 4 cut(s) 710, 1190, 1271, 1413
BspMAI CTGCAG 2 cut(s) 297, 1462
BspMI ACCTGC 1 cut(s) 1638
BspPI GGATC 2 cut(s) 806, 1089
BspT104I TTCGAA 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 1620
BsrDI GCAATG 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 935
BsrI ACTGG 3 cut(s) 355, 865, 1667
BssECI CCNNGG 2 cut(s) 286, 1156
BssMI GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
BssNI GRCGYC 1 cut(s) 672
BssT1I CCWWGG 2 cut(s) 286, 1156
Bst4CI ACNGT 5 cut(s) 125, 619, 647, 746, 893
BstACI GRCGYC 1 cut(s) 672
BstAUI TGTACA 1 cut(s) 935
BstBAI YACGTR 3 cut(s) 89, 1070, 1195
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 2 cut(s) 795, 1134
BstDEI CTNAG 7 cut(s) 111, 131, 203, 869, 1331, 1380, 1416
BstDSI CCRYGG 1 cut(s) 1156
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 6 cut(s) 395, 556, 823, 1491, 1538, 1591
BstFNI CGCG 1 cut(s) 1136
BstKTI GATC 9 cut(s) 36, 103, 147, 202, 505, 814, 1084, 1125, 1252
BstMAI GTCTC 5 cut(s) 14, 111, 206, 878, 1620
BstMBI GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
BstMWI GCNNNNNNNGC 3 cut(s) 401, 1073, 1312
BstNSI RCATGY 1 cut(s) 1306
BstSFI CTRYAG 2 cut(s) 293, 1458
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 2 cut(s) 1085, 1444
BstX2I RGATCY 4 cut(s) 33, 199, 811, 1081
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 4 cut(s) 33, 199, 811, 1081
BsuI GTATCC 2 cut(s) 183, 734
BsuRI GGCC 3 cut(s) 109, 152, 1212
BtgI CCRYGG 1 cut(s) 1156
BtsCI GGATG 6 cut(s) 395, 556, 823, 1491, 1538, 1591
BtsIMutI CAGTG 1 cut(s) 889
BveI ACCTGC 1 cut(s) 1638
Cac8I GCNNGC 2 cut(s) 795, 1134
Cfr13I GGNCC 2 cut(s) 630, 1210
CseI GACGC 2 cut(s) 680, 1011
Csp6I GTAC 2 cut(s) 936, 1453
CviAII CATG 6 cut(s) 261, 515, 1043, 1157, 1303, 1642
CviQI GTAC 2 cut(s) 936, 1453
DdeI CTNAG 7 cut(s) 111, 131, 203, 869, 1331, 1380, 1416
DpnI GATC 9 cut(s) 35, 102, 146, 201, 504, 813, 1083, 1124, 1251
DpnII GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
Eco130I CCWWGG 2 cut(s) 286, 1156
Eco147I AGGCCT 2 cut(s) 109, 152
Eco31I GGTCTC 1 cut(s) 1620
Eco47I GGWCC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 1412
Eco72I CACGTG 2 cut(s) 1070, 1195
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 1386
EcoT14I CCWWGG 2 cut(s) 286, 1156
ErhI CCWWGG 2 cut(s) 286, 1156
Esp3I CGTCTC 2 cut(s) 14, 111
FaeI CATG 6 cut(s) 264, 518, 1046, 1160, 1306, 1645
FaqI GGGAC 1 cut(s) 1487
FatI CATG 6 cut(s) 260, 514, 1042, 1156, 1302, 1641
FauI CCCGC 1 cut(s) 959
FbaI TGATCA 2 cut(s) 144, 502
Fnu4HI GCNGC 3 cut(s) 675, 1074, 1458
FokI GGATG 6 cut(s) 402, 563, 830, 1498, 1545, 1578
Fsp4HI GCNGC 3 cut(s) 675, 1074, 1458
FspBI CTAG 5 cut(s) 585, 678, 713, 809, 1256
GluI GCNGC 3 cut(s) 675, 1074, 1458
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 3 cut(s) 109, 152, 1212
HapII CCGG 1 cut(s) 815
HgaI GACGC 2 cut(s) 680, 1011
Hin1I GRCGYC 1 cut(s) 672
Hin1II CATG 6 cut(s) 264, 518, 1046, 1160, 1306, 1645
HinfI GANTC 4 cut(s) 179, 607, 640, 990
HpaII CCGG 1 cut(s) 815
HphI GGTGA 5 cut(s) 31, 581, 890, 937, 1501
Hpy166II GTNNAC 2 cut(s) 283, 936
Hpy188I TCNGA 9 cut(s) 33, 114, 507, 750, 856, 1392, 1419, 1567, 1625
Hpy8I GTNNAC 2 cut(s) 283, 936
HpyAV CCTTC 6 cut(s) 202, 761, 916, 973, 1169, 1390
HpyCH4III ACNGT 5 cut(s) 125, 619, 647, 746, 893
HpyCH4IV ACGT 6 cut(s) 27, 88, 525, 1069, 1194, 1497
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 1073, 1312
HpyF3I CTNAG 7 cut(s) 111, 131, 203, 869, 1331, 1380, 1416
HpySE526I ACGT 6 cut(s) 27, 88, 525, 1069, 1194, 1497
Hsp92I GRCGYC 1 cut(s) 672
Hsp92II CATG 6 cut(s) 264, 518, 1046, 1160, 1306, 1645
Kpn2I TCCGGA 1 cut(s) 814
Ksp22I TGATCA 2 cut(s) 144, 502
Kzo9I GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
LmnI GCTCC 4 cut(s) 9, 229, 475, 1269
Lsp1109I GCAGC 2 cut(s) 1085, 1444
LweI GCATC 3 cut(s) 498, 682, 993
MaeI CTAG 5 cut(s) 585, 678, 713, 809, 1256
MaeII ACGT 6 cut(s) 27, 88, 525, 1069, 1194, 1497
MaeIII GTNAC 6 cut(s) 134, 647, 823, 887, 1090, 1151
MalI GATC 9 cut(s) 35, 102, 146, 201, 504, 813, 1083, 1124, 1251
MboI GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
MboII GAAGA 7 cut(s) 28, 394, 600, 1118, 1191, 1405, 1519
MflI RGATCY 4 cut(s) 33, 199, 811, 1081
MhlI GDGCHC 2 cut(s) 868, 1233
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 2 cut(s) 52, 1510
MroI TCCGGA 1 cut(s) 814
MroXI GAANNNNTTC 1 cut(s) 404
MseI TTAA 3 cut(s) 519, 561, 660
MslI CAYNNNNRTG 3 cut(s) 426, 1047, 1644
MspI CCGG 1 cut(s) 815
Mva1269I GAATGC 4 cut(s) 406, 1036, 1069, 1222
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 3 cut(s) 401, 1073, 1312
NcoI CCATGG 1 cut(s) 1156
NdeII GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
NlaIII CATG 6 cut(s) 264, 518, 1046, 1160, 1306, 1645
NlaIV GGNNCC 4 cut(s) 710, 1190, 1271, 1413
NmuCI GTSAC 2 cut(s) 823, 887
NspI RCATGY 1 cut(s) 1306
NspV TTCGAA 1 cut(s) 959
OliI CACNNNNGTG 1 cut(s) 426
PceI AGGCCT 2 cut(s) 109, 152
PctI GAATGC 4 cut(s) 406, 1036, 1069, 1222
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 3 cut(s) 179, 640, 990
PflFI GACNNNGTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 676, 1075, 1459
PleI GAGTC 1 cut(s) 601
PmaCI CACGTG 2 cut(s) 1070, 1195
PmlI CACGTG 2 cut(s) 1070, 1195
PpsI GAGTC 1 cut(s) 601
Ppu21I YACGTR 3 cut(s) 89, 1070, 1195
Psp1406I AACGTT 1 cut(s) 27
PspCI CACGTG 2 cut(s) 1070, 1195
PspN4I GGNNCC 4 cut(s) 710, 1190, 1271, 1413
PspPI GGNCC 2 cut(s) 630, 1210
PstI CTGCAG 2 cut(s) 297, 1462
PsuI RGATCY 4 cut(s) 33, 199, 811, 1081
PsyI GACNNNGTC 1 cut(s) 256
RsaI GTAC 2 cut(s) 937, 1454
RsaNI GTAC 2 cut(s) 936, 1453
RseI CAYNNNNRTG 3 cut(s) 426, 1047, 1644
SaqAI TTAA 3 cut(s) 519, 561, 660
SatI GCNGC 3 cut(s) 675, 1074, 1458
Sau3AI GATC 9 cut(s) 33, 100, 144, 199, 502, 811, 1081, 1122, 1249
Sau96I GGNCC 2 cut(s) 630, 1210
SchI GAGTC 1 cut(s) 601
SduI GDGCHC 2 cut(s) 868, 1233
SfaNI GCATC 3 cut(s) 498, 682, 993
SfcI CTRYAG 2 cut(s) 293, 1458
SfuI TTCGAA 1 cut(s) 959
SinI GGWCC 1 cut(s) 630
SmiMI CAYNNNNRTG 3 cut(s) 426, 1047, 1644
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SseBI AGGCCT 2 cut(s) 109, 152
SsiI CCGC 4 cut(s) 7, 675, 966, 1136
SspMI CTAG 5 cut(s) 585, 678, 713, 809, 1256
StuI AGGCCT 2 cut(s) 109, 152
StyI CCWWGG 2 cut(s) 286, 1156
TaaI ACNGT 5 cut(s) 125, 619, 647, 746, 893
TaiI ACGT 6 cut(s) 30, 91, 528, 1072, 1197, 1500
TaqI TCGA 6 cut(s) 99, 347, 408, 915, 959, 1404
TatI WGTACW 1 cut(s) 935
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 3 cut(s) 179, 640, 990
Tru1I TTAA 3 cut(s) 519, 561, 660
Tru9I TTAA 3 cut(s) 519, 561, 660
TscAI CASTG 1 cut(s) 896
TseFI GTSAC 2 cut(s) 823, 887
TseI GCWGC 2 cut(s) 1073, 1457
Tsp45I GTSAC 2 cut(s) 823, 887
TspDTI ATGAA 2 cut(s) 426, 1658
TspRI CASTG 1 cut(s) 896
Tth111I GACNNNGTC 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 630
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 3 cut(s) 44, 374, 1386
XceI RCATGY 1 cut(s) 1306
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 5 cut(s) 585, 678, 713, 809, 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.