Rorug01G0187500

Pentatricopeptide repeat-containing protein At2g44880-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008546
Physical Location & Seq
Forward (+)
25903331 .. 25923427
20097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0187500.1

Sequence Viewer

Length: 8961 bp
ATGGCTTCCATGGTGATCCAAACCTCCTCAACTTCTACTCCTCCTCCTCCACCTTTGAACCATCCAATAAAATATGATGTTTTTCTCAGTTTTAGAGGTGCGGACACCCGCACAAAGTTTACAGACCATTTATACAAGGAATTACATGTTCGGGAAGGCATCCCCACCTTTAGGGATTCTGAAGAGCTCGAGAAGGGAGAAGACCTTTCACAACTCTTTGAGGCAATTGAAAAATCGAAAATTGCAGTTGTCATTCTTTCAAGTAACTATGCTTCTTCGACATGGTGTTTGAGAGAAGTTGCCAAGATTGCCGAATGCAGAAATAAGACCGGACTCATCGCTTTGCCGGTATTCTATGAGATCGAACCAACCCAGGTACGAAGCCAGACAGGAAGTTTCAAGAAATACTTTGATGAACATGAAGAGGAATACAAGGACAGCATAGAGAAGGTCGATATTTGGAGGAAAGCGTTGACTGAAATAGGTGGTCTCTCCGGATGGGATTTAAAAGACAGGTCGGAAACCTCTGTTATTGAAGAGATAGTAAAATGTGTCGAGACAAAATGGAAGCTCAAATTTAATCCTGCATTCTCAATTGAGGATTCCAAGGATTTTGTTGGGATGGATCGTGTGAGGAAAATCGAATTGGACTTGGATATAGAGGCATTGGATAAAATCGGTGTCATTGGAATTTGCGGGATGCCAGGAGTAGGTAAGACAACAATTGCAGAACTTGTGTTTCGAAGAAATTGGCACCATTTTGAAGCTAACAGTTTCCTTGCTAATGTTGGAGAGATACGTGAGACGAATGGATTATTGCATATACAGAAGCAACTTTATCGCGACTTGTTGGATAGCAAAGTTATAACTATGAATGATGTCCCTGGGGAAAGGAGAAAATTGCTTCACAAAAAGGTGCTTATCATTCTTGATGGTGTGACTGAGTTAGAATCTGTTGTTGAGCTAGCAGGAAGAGGTGGTGAAGAATTAATCAATTGGTTTGGTCGAGGCAGTAGAATCATTATAACCGCAATAGATGAAAAGCTGTTGAAGGATTATGAGGCAAAAACACACAAGGTTGAGACACTTTCTGAAAATGAGCCTCGTGTGCTCTTCTTCCGGAAAGCCTTCAACAAAGACCATCCTCTGCATGATTGTACTGAGTTGGACTTCAAGTTCAAGATAGACCATACTATGCGTCAGTATGCTGAGTTGGCTTACAAATTTGTGGATTTAACTGACGGTCTTCCTTTGGTTATTGTAGTTTTGGGAACCTTGTTATTGAACAGATCTTTGGGTGAATGGGAAGCTCAACTTCGCACACTAGAAGCTAATAATGATGCTAATGATGAGAAAACTTTTGGGATTCTTAAGCTATGTATTGATGGATTACGTGATTTAGGAAAGGAAATTTTCTTGGACATTGCATGCTTCTTTAAAGGAGATGACAGAAAACGTGTAGAAAGGATATTTGAAAGCTGCGGCCTCAATCCAGGCATTGAATTGAACATTCTCGTCGAGAAATCTCTAGTGAGAATTGTGGGAGGAAAATTGTGGATGCATGATTTAATACAAAAAGCTGGCCATAAAATGGTTATTCGAAAATCCGAATTACCAGAGAATCGCAGCAGATTGTGGGATCGTGAAGAAGCCGTTTCGGTACTTAAACATAATAAGGGGACAAGCGCTGTCAAAGGTATTTTCTTAAGCTCACCTCAACTCTGCAAAGTGCGATTGAAGGCCGACTCATTTTCAAAAATGGACTTTCTTAGATTGTTGAAAATAAAGAATGTGAAATTTTCTGGATGCCATGAATACGATCTTTCAAATAAATTAAGCTTCTTGGAATGGCATGACTTTCCTTCAACATTTCTGCCGTCTGAGTTTGAACCGAAGAATCTTGTTGAACTGCAGTTGCCTCGAAGCCAAATAAAGCAGTTATGGGAGGATAATATGACGCCTCCTTTGGAAAAGTTGGTTGTAATGGACCTTAGTGATTGCAAAGAGTTGACCAGTACCCCAGACTTCCAGTCAGTGCCAAATCTTGAGAGTCTAGTTCTCAAAGGTTGTAAAAGATTGTCTGAGGTACGCCTACCCAATGGAGATCAACTCCAAAAGCTCATTTCATTGAATCTGGAAGGTTGCGAATCTCTTAGTAGTCTTCCCAACGGTATCTGCAGCATGAGATCTCTCGAAACTTTCATTCTTTCGGGATGTTCCAGACTTGCAAATCTTCCGGAGAAGTTTGGGGAAAACATGAAACAATTATCCGGACTTGATTTGGAGGGGACAGCTATAAAAGGGCTACCAACATCAATTGAGCACTTGACTGGTCTTATTCGGCTCAATCTAAAAGACTGCAAGAACCTTTTAAGTCTGCCAGCTGTCATTTGTAGTTTGACAAAACTTCAAGTTCTCAACCTATCTGGCTGCTCAAATCTTGCTGCTTTGCCAGAAAACCTGGGAAGTTTAGTAGGTTTGAAGGAACTTGATGCAAGCAGAAGTTGTATAAGACAAGTACCTTCCTCCATTCTACTTCTGAAGGACCTTGAAAGTTTATCTTTCTTTGGATGTGGAGGTTTGCCGCCATCAGCATGTCCCATGGGTTTGCAGTTGCCTGCTTCATTCTCGGGATTAGATTCTTTGACAAAATTGGATCTAAGCTATTGCAAACTGTCTGGTGGAGCAATCCCTGCTGATCTTGACTGCTTATCTTCACTTAATCATCTAAATTTAAGTGGGAACAATTTAGTGAGTGTACCGGAAAGCATCACTAAACTTTCTAACCTTGAAGTCCTTCTATTGAATGATTGTAGCGAGCTTCGATCATTACCACATCTTCCACCAAGTATACGACGTGTTTTTGCACGGAATTGTCCTCTGCTCAGAACTTATTCAAATAAATTAACTGTGATGAGTTCAACTGCTACAGGATTTTGTTTCATAAATTCCCAAAGTAGTGAAGAACGTAAACTTCAGAGGATTCCGATATCAGAACAACGTCTTCTGCGGCCTCATTTGCAAAAGTTCTATGAGGATATAATTTATAAAGGTTGTGGTTTTGAATATATTACTCCTCACACTGAAATTCCATCATGGTTCCGTCACCCGGGTAGAGAGTCGTCTGTAACACTCCAGCTACCTCTAGATTATTTCGATGGCAAACGTGAGTTGATGGGACTTGCTCTATGTTTTGTTTATGAAGCGCAGGAGCATGAGAATATGGAATCAACAGCTTCTAGCATCTCGGCTGAGGAAGTAAAATATAGTTGCAATTACAGTTGTCGCTTGTCCACATATGGAGGTCCTCATGATGACTATCCCCTCCTCGTGTGTAAACTCATGGATCATGATTATGTTGGATCTTTTCGACATTGGGTCTATATAGCAGGAGGACTGTTTGCAGAAAGGTTAATCAAACGCTCTGTTCGAGCTGAAGTTATAATCAACAGACCAGGGGTGAAAGTGAAAGCCTGTCGGGCAAGTCTAATATACTCTGGAGGTGTGACAGAATTTGTTTGGAATTTGAATCTCCGTGATGAAATTCATGTGAGAAATCTGTCGAGTGGGGATCAAAAGGAAAAATCTGACGATCAGTTTAATAAACAAGAACAAGAAACTGTTTGTGATCATCCGCTGGAACTAAAACATGTACCTGTCCAATATCAGGCCCAAATGAACCGCATGATCTCATTGTATGATACTAAACGAAGTAACAAGCGATCCCTTGACCATGATGCCGGATCATCTAGTAGAGCCAGCAGCAGCACTGAAGAACCTCAACAAAGATTAGAAATATCAAAATCCAACGATCAGTCCAACAAACAAGAGCAAGAGACTAATTATGGTTGTGCTACAGACAAAAGATTCTTGATTGAAATTGAACCACACACTTCAAGAGAGAGTTCCACTTCAACCACGATCTTTGATGATCAATTGGGAACAAACGTAGAGTCATTCCTTGAGGAACTATTTGAGCTAGGAGGCAAAGCACGTCGCAACGATTTAGGTTTCATTTTCCCTCTCCGAGCAATTTTGCCGTGGTTTAATCATCAATGCATTGGGAATGTATTCATTCCAGAAAAAGAAAGAATTGGGAGTTCAGTAAGCCTACCTCTTCCTCTAGAATTGTATAACAATAAGGAATGGGTAGGATTTGCTGTATATGTGGCCTTCACTCTGCCTCCAGGTGTTTCACTTGAAAAATCATGTCGCTTCAAGTGTGTATTGTATACTAGTGATGGACGTTTTCATTGCCTAAAATTATTGGCGGCTTGTGAAGACAATTTTAGGGGAGCACATCAACTCCTTGTCATCCACGTACCACGAGGGCGTTTTAAGAAACAGTCGAATCAGTGCTGGGGAATTCGTGCTGCTTTTGTGTCCTCAACTCCAAGTGTGGAGGCTGAGATGTGTGGGATCCGTGTAGTATACGAGAAAGATTTGAAAGGGTTAATCCAAACAATTACCCAATGCACCATAAGAAATCCGCCTGCAGCTTACTATGGACTTGCTCATATATACGTGCCAGATGGAAGAGAGTCATTCATGTTAGCTATTATGGAGGATAATTTGATTGAAGCGGCTAAATCCAGCGAAAGGACGCTTTCAACCTTGCAAGAGCCTTTAACAGATTCAAAGTTCCTCAGTGGTCCACAAATTTATTTACAGATAGAGGCTGGAACTCCATACATGCCACTCAAGTTCAAAGAATCCATAGTTGACTGGAAAAAAAACCTTGAGCTTTTGCTTGAATGTTTCTTTACCCTTTCTGATCTATCTATTAATCTCACTCTTAACAGAAGCAGAACCTCTTTCTCAAGGGATTTTGATCCTTGCTCTGGTATTTTCCTTCAAAAGGAAATTTCAGAGTGGTTCATACCACAATATCCCTCTGAGAGTATGGTGTTGGAACTTCCTCCAAATTTGGAAAATGATGTGAATTTCATGGGACTTGTTGTATGTGCTTCCTTTTCAGTCCATGCCTCTGAAGCTGACGTCCTCAAATATCTGAAACCAGGACTCCATCCCCAGCTTACGTGCCATTTCCAAAATTTGAGTTCTGATCATTCTTATGGTCTTAGAGCTCCCATGATTGATGCCAGTCTCGAGAACAAATTCATGTGGTTATATCGCAGAGGATTCATTTGGCAGGAATACCTACCCCGTGACTTATTTAAGGATCTGCTGCATAAAGATACTTCAGTCAAGGTTTCATTTAGGAGTGATTCTTCCCAACTCTTGGAGGTGCAGAAATGTAGCTTCCGCATCTTATACAAGCAAGATGTGGAAAAATTCAAGCATATATTAATACAGTGCTTTACCTCCTTCTTTGACAATGACAATGTCATTCGAGCATATTTAGTAGAGGATTCTGGAGATTACATGCAAGCCCTTGACCATGATGCAGGATCATCTAGTAGAACTGGCAGCAGTACTGAAGAACCTGTTCAAATATTAGAAAGATCAGAATCCAACGATCAGTCCGATGAGCAAGTGCATGAAACTAATTCTGACCTTACACATGTAGCAGAATGTGGTTATGCTTCAGACACTAGATTCTTAATTGAAATTGAACCACACACTTCAAGAGATAATTCAAGTTCAACCAAGATTGTTGGTGATCAATTGAGAAAAAATGTAGAGTCATTCCTTGAAGAACTGTTTGAGCTAGGAGGCAAAGCACGTCGCTATGATTTAGGTTTCATTTTCTCTCTTCGAGCAATTTTACCGTGGTTTAAACATCAAGGCATTGGGAACGTGATAGGTGTACCTCTTCCTTTAGAATTGTATAACAACAAGGAATGCATAGGATTTTCTCTATATGTGATCCTCACTCTGCCCCCAGGTGCTTGGCTTAAAGAAGGTTCTTGTTTTAAAATTGTGTTGTTTACTGAAGAGCCAAGGTATTGCCTAAACTTAGCGCCCACTGGTGAAGACAATTTCATGGGTTCACATCGGCTCCTGGTTATTCACATACCACAAAGACATTTTGGAGAACAGTTGAACCACTGGAGTACCATTCGTGCTTCTTTTGAATCCCTTATGCCAGGTGTGGAGGTTGAGATGTGTGGTATGCGGGTAGTATACCAGGAAGATTTAAAAGGGTTAATCCAAACCATTACCAACTGCACAATATGCAGTCCCTCTGCTTACTATGGACTTGCCCAACCATTTGTGCCGGGAAGGTGGTCTGAAGTGCTCACACACATGAGGAATAGTTTGGTTGAAGCAGCTAAATCCAGCAAGAGGAAGTTTTCAACCTTGCAAGAGCCTTTAACAGATTTAAAATCCATCCAAGGAATTCAGTTAATGATACTTGCTGTAACTCCATTTACTTCACTCAAGAAAACAGAAACAGTAGTCAAGTGGAAAAGAAACCTTAAGCTTTTGCTTCAACGTTTCTTAACCCTTTCTGGTCTATCAATAAGTCTCATTCTTAGAGGAGGCAGAATTTCTTTCTCAGAGGATCATAATCTTCATACTGGTATTTTCTTTCAAAAAGAAATTCCAGACTGGTTTTCACCACACCACTCTGCCAGTATGGTGCCCGAACTTCCTCCAGATTTGGAAAATGATATGAATTTGATGGGTTTTGTTGTATGTGCTGCCTTTTCAGTCCAGGCCTCTAAAGCTGATGTCCTCAAATATCTGGATTCAGGACACCATCCCGAACTTATGTGCCATTTCCAATCAAGTTCTGACTATTCTGGTGTAGTAGTTCGTAAAATGGTTGGCATCAGTCCGAAGAACAAATTCATGTGGTTTTATCGTAGAGGATTCATTTGGCTGCATTACATACCCCGTCACAATGTTGAGCATCTCTTCAATAAACTATCTGAACTACAGGTTTCATTTGAGAGTGATTCTTCCCAAGTATTGGCGGTACAGAAATGTAGCTTCCGTTTCTTATACAAGCAAGATGTAGAAGAATTTAAGCAAACAATAATAGAGTGCTTTACCTCCTTCTTTGACAACGAGGATATCATTAAGCGATATATAGAAGAAGAGGACTCTGGACATAATAAGTGGCCAAATCATGGAGATGAGATGGGACCATCTAGTACAACTGGTGGCAATATTGTCATTGAGCAAACTCACAATGAGAGATTAGATGTACCCATAACTGATGGGAAGGGGAAAGGCCCTTCTGATGTCTCCTCAGTTGAAGCAGATGAGAGAGATTATTATGGAACTACACCTGTTTGTCAGGGTGAAACTAGTGGGAAAGGCACTACACCCGTTTGTCAGGGTGAACGAGGTTCTGCATCCAGTACTTCTCCGGAGCTAATGCCATTTTTCATTGAAACTGAACCTCCAGCTTCAACGGATTCAAGTTCAACCGAAGTTACACGGCAACTGAGAATGATAGAGTCATCGCTTGAACAACTATTTCAGGGGGGCAGCGCACGCCACTATAATTATGGCTTTATTTTATCTCCTAGAGTCAGAGCAGTTTTGCCGTGGTTTAGTCATCAAAGCATTGGGAAAGAAGTAAAAATTAATGCTCCTCCGGATTTGTATAAAGATAATAAATGGGTAGGCTTTGCTCTATATGTTGCCTTTACTCAGTCTCCAGGTTCACATAGTTGCTCCATGTGTAGTTTGTTTGTCTTCCCAGGACATTCAGTACATTCCATAAGCACATGCATCGATGAAGACCAATTTTCCGGATCACGAAGACTTTGTTTCTTTCATATACCACGTGCGCGCTTCCCTGAGCAGTGTGATCAGTGTCCTGTCATTTTTGCTCGGTTTGATTTCTTTAGCCCAGGTTCGGAGGTTGAAATGTGTGGGATCCGTGTAGTATACGATGAAGATATAAAAGGGCTAGTCCAAACAATTACTCAGAGCACTATCAGGAGTCCTCCGCCTGTTTACTTCTCCCAAGATGAAAAAACAGAAATTTTTTCCCCAGGGAGGCTTGTGCTGCCCATACATATGGCCTCGAGTTTGGTTGCTCACTCCAATGCCTGCATCCTGCCCACACGAAGCGCACAAAGTCCACTGGTTCCATCCATGAAATTTGGTAAAGTAAACTTGGTCATACATGGTGACACTCCATTCAAGAAAGATACAACCGAGTGGAGAAGAAACTTTGAGATTTTGCTTCAATATTTCTTCAGGCTTGATCTATCCGTAGAACTCGTTCTCGAAGAAGACACCATCGCCTTCTATCAGGATTGCTTCAATCATGCTTGTCTTTTCCCTCAAAAGAAAATTCCAGATTGGTTCAAAGAAAAATCTCACACATCTACGTATGTGTACCCACTTCCCCATGATTTGGAAAGTGACAACAATTGGATGGGACTTGTTGTATGTGCTGCCTTTTCAGTACACCCACTTCCAACTGACCTTGGCAATTATAAGGACTCAGAACTGAGTTCTGATGTTAAGTGCAATTTTCGGTGGAGTTCTGATCCTTCTCGATTCATTTCTATCAATGAGGGTGTGTTCGGCGTCAGGCCAAACAACAAATTCATGTGGTTATCTCGTAGAGGATTCATTTGGCTGCAATTCATATCTGGTGAAATTTTTAAGTTTGCCATGGATAAACATCGTCGTCAACTAGAAGCTTCATTTGAGAGTGGTTCCCCAGCTTTGTCGGTACAGGAATGTAGCTACCGAATCTTATACAAGCAAGACTTGCAAGAATATAAGCAAACAATTATACAGTGCTTCGCCTCTGTCTTTGACAACTTGGATCACAGTCTACCATATAAGGAAGACGATTCTCGACATAACGAGGAGCCAATTCATGAAGATGATCAGATGGACTCTTTAGGAACTACAAGCTTTTCTGAGGACACCCCCCATGGAAGAGAAACAACAGTGGTTCTTCCTGAGAGTGAAACCAGTGGAAATTCCTTTAATCGTTTCAACAGCATACTGTTTGAGCCCATTTTGTACGACAACAAAACGGCAGTAATTTTCGCTGATTTTCGAGGATGGGAAGAATGA

Protein Analysis

2986

Amino Acids

338.84

Weight (kDa)

5.8

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 191 2.5e-46 TIR domain
TIR_2 PF13676 27 - 148 6.6e-13 TIR domain
NB-ARC PF00931 224 - 380 1.9e-10 NB-ARC domain
WHD_ROQ1 PF23282 469 - 534 1.3e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 729 - 814 4.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 742 - 810 4.1e-07 Leucine-rich repeat region
LRR_13 PF23286 816 - 981 1.9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1026 - 1088 5.4e-08 C-JID domain
C-JID PF20160 2167 - 2310 1.7e-06 C-JID domain
C-JID PF20160 2489 - 2608 9.8e-06 C-JID domain
C-JID PF20160 2736 - 2883 1e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 866, 1025, 3048, 3443, 8367
AatII GACGTC 1 cut(s) 5001
AccB1I GGYRCC 2 cut(s) 753, 6547
AccB7I CCANNNNNTGG 5 cut(s) 1591, 5242, 6880, 7040, 8284
AccI GTMKAC 6 cut(s) 2851, 4232, 4431, 6087, 7809, 8713
AccII CGCG 2 cut(s) 843, 7711
AccIII TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
AclI AACGTT 1 cut(s) 6400
AcoI YGGCCR 2 cut(s) 1582, 7031
AcvI CACGTG 1 cut(s) 7706
AcyI GRCGYC 3 cut(s) 1958, 4998, 8460
AfeI AGCGCT 1 cut(s) 1687
AflII CTTAAG 3 cut(s) 1370, 1705, 6383
AflIII ACRYGT 5 cut(s) 145, 1456, 2857, 3649, 5524
AhdI GACNNNNNGTC 2 cut(s) 2029, 3377
AhlI ACTAGT 2 cut(s) 4235, 7220
AjiI CACGTC 3 cut(s) 2858, 3995, 5687
AjuI GAANNNNNNNTTGG 4 cut(s) 629, 661, 1277, 1309
AloI GAACNNNNNNTCC 2 cut(s) 1160, 1192
Alw21I GWGCWC 7 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 7856
Alw26I GTCTC 9 cut(s) 494, 551, 797, 1076, 3830, 5111, 6437, 7162, 7578
Ama87I CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
Aor13HI TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
Aor51HI AGCGCT 1 cut(s) 1687
ArsI GACNNNNNNTTYG 6 cut(s) 271, 303, 1948, 1980, 3938, 3970
AseI ATTAAT 4 cut(s) 989, 4785, 5309, 7503
Asp700I GAANNNNTTC 8 cut(s) 1127, 2796, 2893, 4070, 5117, 5448, 6755, 8148
AspLEI GCGC 7 cut(s) 1688, 3208, 5926, 7409, 7711, 7713, 7997
AspS9I GGNCC 7 cut(s) 1987, 2545, 3305, 3670, 4652, 7055, 7145
AsuC2I CCSGG 3 cut(s) 3110, 3111, 6183
AsuII TTCGAA 2 cut(s) 742, 1600
AsuNHI GCTAGC 1 cut(s) 964
AvaI CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
AvaII GGWCC 5 cut(s) 1987, 2545, 3305, 4652, 7055
BaeGI GKGCMC 1 cut(s) 6552
BaeI ACNNNNGTAYC 4 cut(s) 5190, 5223, 6001, 6034
BalI TGGCCA 2 cut(s) 1584, 7033
BamHI GGATCC 2 cut(s) 4419, 7797
BanI GGYRCC 2 cut(s) 753, 6547
BanII GRGCYC 3 cut(s) 189, 5089, 8901
BarI GAAGNNNNNNTAC 4 cut(s) 256, 288, 8328, 8360
BauI CACGAG 3 cut(s) 1104, 3329, 4326
BbrPI CACGTG 1 cut(s) 7706
Bbv12I GWGCWC 7 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 7856
BbvCI CCTCAGC 1 cut(s) 3252
BceAI ACGGC 6 cut(s) 1637, 1861, 4024, 7370, 7447, 8937
BcgI CGANNNNNNTGC 6 cut(s) 821, 855, 1901, 1935, 7633, 7667
BclI TGATCA 6 cut(s) 3628, 3931, 5065, 5623, 7729, 8767
BcnI CCSGG 3 cut(s) 3110, 3111, 6183
BcoDI GTCTC 9 cut(s) 494, 551, 797, 1076, 3830, 5111, 6437, 7162, 7578
BcuI ACTAGT 2 cut(s) 4235, 7220
BfmI CTRYAG 6 cut(s) 1910, 2176, 2928, 3855, 4494, 6845
BfoI RGCGCY 2 cut(s) 1689, 5927
BfrI CTTAAG 3 cut(s) 1370, 1705, 6383
BglI GCCNNNNNGGC 1 cut(s) 3479
BglII AGATCT 2 cut(s) 1289, 2186
BmcAI AGTACT 2 cut(s) 5437, 7276
Bme18I GGWCC 5 cut(s) 1987, 2545, 3305, 4652, 7055
BmeRI GACNNNNNGTC 2 cut(s) 2029, 3377
BmeT110I CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
BmgBI CACGTC 3 cut(s) 2858, 3995, 5687
BmgT120I GGNCC 7 cut(s) 1987, 2545, 3305, 3670, 4652, 7055, 7145
BmtI GCTAGC 1 cut(s) 968
BplI GAGNNNNNCTC 2 cut(s) 2094, 2126
BpmI CTGGAG 8 cut(s) 3119, 3519, 4170, 5397, 6034, 6546, 7302, 7560
Bpu10I CCTNAGC 2 cut(s) 3252, 7719
Bpu14I TTCGAA 2 cut(s) 742, 1600
BpuEI CTTGAG 6 cut(s) 2066, 3983, 4685, 4760, 4804, 6329
BpuMI CCSGG 3 cut(s) 3110, 3111, 6183
Bsa29I ATCGAT 1 cut(s) 7653
BsaAI YACGTR 7 cut(s) 800, 1394, 4321, 4525, 5040, 7706, 8259
BsaBI GATNNNNATC 5 cut(s) 3318, 4830, 5470, 6474, 8556
BsaHI GRCGYC 3 cut(s) 1958, 4998, 8460
BsaI GGTCTC 1 cut(s) 494
BsaWI WCCGGW 9 cut(s) 329, 494, 1119, 2236, 2270, 2761, 7282, 7513, 7670
Bse118I RCCGGY 1 cut(s) 346
Bse3DI GCAATG 2 cut(s) 1422, 4252
Bse8I GATNNNNATC 5 cut(s) 3318, 4830, 5470, 6474, 8556
BseAI TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
BseCI ATCGAT 1 cut(s) 7653
BseJI GATNNNNATC 5 cut(s) 3318, 4830, 5470, 6474, 8556
BseMI GCAATG 2 cut(s) 1422, 4252
BsePI GCGCGC 1 cut(s) 7709
BseSI GKGCMC 1 cut(s) 6552
BseYI CCCAGC 3 cut(s) 4359, 5031, 8596
BsgI GTGCAG 2 cut(s) 5270, 6115
Bsh1236I CGCG 2 cut(s) 843, 7711
BshNI GGYRCC 2 cut(s) 753, 6547
BshVI ATCGAT 1 cut(s) 7653
BsiHKAI GWGCWC 7 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 7856
BsiHKCI CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
BslFI GGGAC 9 cut(s) 866, 1693, 2302, 2583, 3192, 4965, 6129, 7068, 8322
BsmAI GTCTC 9 cut(s) 494, 551, 797, 1076, 3830, 5111, 6437, 7162, 7578
BsmBI CGTCTC 1 cut(s) 797
BsmFI GGGAC 9 cut(s) 866, 1693, 2302, 2583, 3192, 4965, 6129, 7068, 8322
BsmI GAATGC 3 cut(s) 320, 587, 5810
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
Bsp119I TTCGAA 2 cut(s) 742, 1600
Bsp1286I GDGCHC 9 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 6552, 7856, 8901
Bsp13I TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
Bsp19I CCATGG 4 cut(s) 9, 2601, 8547, 8815
Bsp68I TCGCGA 1 cut(s) 843
BspDI ATCGAT 1 cut(s) 7653
BspEI TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
BspFNI CGCG 2 cut(s) 843, 7711
BspHI TCATGA 3 cut(s) 3310, 3349, 8758
BspMAI CTGCAG 3 cut(s) 1914, 2180, 4498
BspOI GCTAGC 1 cut(s) 968
BspQI GCTCTTC 3 cut(s) 177, 1118, 5892
BspT104I TTCGAA 2 cut(s) 742, 1600
BspT107I GGYRCC 2 cut(s) 753, 6547
BspTI CTTAAG 3 cut(s) 1370, 1705, 6383
BspTNI GGTCTC 1 cut(s) 494
BsrDI GCAATG 2 cut(s) 1422, 4252
BsrFI RCCGGY 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 346
BssHII GCGCGC 1 cut(s) 7709
BssNAI GTATAC 5 cut(s) 2852, 4233, 4432, 6088, 7810
BssNI GRCGYC 3 cut(s) 1958, 4998, 8460
BssSI CACGAG 3 cut(s) 1104, 3329, 4326
BssT1I CCWWGG 8 cut(s) 9, 606, 2601, 5903, 6298, 8356, 8547, 8815
Bst1107I GTATAC 5 cut(s) 2852, 4233, 4432, 6088, 7810
Bst2BI CACGAG 3 cut(s) 1104, 3329, 4326
BstACI GRCGYC 3 cut(s) 1958, 4998, 8460
BstAFI CTTAAG 3 cut(s) 1370, 1705, 6383
BstAPI GCANNNNNTGC 3 cut(s) 2693, 6138, 8647
BstBAI YACGTR 7 cut(s) 800, 1394, 4321, 4525, 5040, 7706, 8259
BstBI TTCGAA 2 cut(s) 742, 1600
BstDSI CCRYGG 7 cut(s) 9, 2601, 4040, 5732, 7463, 8547, 8815
BstENI CCTNNNNNAGG 1 cut(s) 4856
BstFNI CGCG 2 cut(s) 843, 7711
BstH2I RGCGCY 2 cut(s) 1689, 5927
BstHHI GCGC 7 cut(s) 1688, 3208, 5926, 7409, 7711, 7713, 7997
BstMAI GTCTC 9 cut(s) 494, 551, 797, 1076, 3830, 5111, 6437, 7162, 7578
BstNSI RCATGY 8 cut(s) 149, 1431, 2598, 3653, 4696, 5389, 5528, 7650
BstSFI CTRYAG 6 cut(s) 1910, 2176, 2928, 3855, 4494, 6845
BstSLI GKGCMC 1 cut(s) 6552
BstSNI TACGTA 1 cut(s) 8259
BstUI CGCG 2 cut(s) 843, 7711
BstX2I RGATCY 7 cut(s) 1289, 2186, 2656, 3362, 4419, 5182, 7797
BstXI CCANNNNNNTGG 2 cut(s) 5853, 7942
BstYI RGATCY 7 cut(s) 1289, 2186, 2656, 3362, 4419, 5182, 7797
BstZ17I GTATAC 5 cut(s) 2852, 4233, 4432, 6088, 7810
Bsu15I ATCGAT 1 cut(s) 7653
BsuTUI ATCGAT 1 cut(s) 7653
BtgI CCRYGG 7 cut(s) 9, 2601, 4040, 5732, 7463, 8547, 8815
BtgZI GCGATG 3 cut(s) 322, 7362, 8152
BtrI CACGTC 3 cut(s) 2858, 3995, 5687
BtsI GCAGTG 1 cut(s) 7730
BtuMI TCGCGA 1 cut(s) 843
CciI TCATGA 3 cut(s) 3310, 3349, 8758
CfoI GCGC 7 cut(s) 1688, 3208, 5926, 7409, 7711, 7713, 7997
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 7 cut(s) 1987, 2545, 3305, 3670, 4652, 7055, 7145
Cfr9I CCCGGG 1 cut(s) 3109
ClaI ATCGAT 1 cut(s) 7653
CseI GACGC 4 cut(s) 1187, 1966, 4612, 8449
CspCI CAANNNNNGTGG 2 cut(s) 3907, 3942
DraI TTTAAA 6 cut(s) 507, 1438, 5740, 5878, 6102, 6288
DriI GACNNNNNGTC 2 cut(s) 2029, 3377
EaeI YGGCCR 2 cut(s) 1582, 7031
Eam1105I GACNNNNNGTC 2 cut(s) 2029, 3377
EciI GGCGGA 2 cut(s) 4479, 7860
Ecl136II GAGCTC 2 cut(s) 187, 5087
Eco105I TACGTA 1 cut(s) 8259
Eco130I CCWWGG 8 cut(s) 9, 606, 2601, 5903, 6298, 8356, 8547, 8815
Eco147I AGGCCT 1 cut(s) 6626
Eco24I GRGCYC 3 cut(s) 189, 5089, 8901
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 2 cut(s) 2991, 6985
Eco47I GGWCC 5 cut(s) 1987, 2545, 3305, 4652, 7055
Eco47III AGCGCT 1 cut(s) 1687
Eco53kI GAGCTC 2 cut(s) 187, 5087
Eco72I CACGTG 1 cut(s) 7706
Eco88I CYCGRG 5 cut(s) 188, 2629, 3109, 5108, 7948
EcoICRI GAGCTC 2 cut(s) 187, 5087
EcoNI CCTNNNNNAGG 1 cut(s) 4856
EcoO109I RGGNCCY 3 cut(s) 2545, 3305, 7145
EcoRI GAATTC 2 cut(s) 4365, 6303
EcoRV GATATC 2 cut(s) 2991, 6985
EcoT14I CCWWGG 8 cut(s) 9, 606, 2601, 5903, 6298, 8356, 8547, 8815
EcoT22I ATGCAT 4 cut(s) 1563, 4061, 5810, 7652
EcoT38I GRGCYC 3 cut(s) 189, 5089, 8901
ErhI CCWWGG 8 cut(s) 9, 606, 2601, 5903, 6298, 8356, 8547, 8815
Esp3I CGTCTC 1 cut(s) 797
FalI AAGNNNNNCTT 8 cut(s) 392, 424, 1299, 1331, 2545, 2577, 8630, 8662
FaqI GGGAC 9 cut(s) 866, 1693, 2302, 2583, 3192, 4965, 6129, 7068, 8322
FauI CCCGC 3 cut(s) 116, 689, 6072
FauNDI CATATG 2 cut(s) 3298, 7941
FbaI TGATCA 6 cut(s) 3628, 3931, 5065, 5623, 7729, 8767
FblI GTMKAC 6 cut(s) 2851, 4232, 4431, 6087, 7809, 8713
FriOI GRGCYC 3 cut(s) 189, 5089, 8901
GlaI GCGC 7 cut(s) 1687, 3207, 5925, 7408, 7710, 7712, 7996
GsaI CCCAGC 3 cut(s) 4363, 5035, 8600
GsuI CTGGAG 8 cut(s) 3119, 3519, 4170, 5397, 6034, 6546, 7302, 7560
HaeII RGCGCY 2 cut(s) 1689, 5927
HgaI GACGC 4 cut(s) 1187, 1966, 4612, 8449
HhaI GCGC 7 cut(s) 1688, 3208, 5926, 7409, 7711, 7713, 7997
Hin1I GRCGYC 3 cut(s) 1958, 4998, 8460
Hin6I GCGC 7 cut(s) 1686, 3206, 5924, 7407, 7709, 7711, 7995
HinP1I GCGC 7 cut(s) 1686, 3206, 5924, 7407, 7709, 7711, 7995
HincII GTYRAC 4 cut(s) 474, 2010, 4723, 8567
HindII GTYRAC 4 cut(s) 474, 2010, 4723, 8567
HindIII AAGCTT 4 cut(s) 1837, 6386, 8574, 8794
Hpy99I CGWCG 5 cut(s) 1520, 2859, 3999, 5691, 8565
Hsp92I GRCGYC 3 cut(s) 1958, 4998, 8460
HspAI GCGC 7 cut(s) 1686, 3206, 5924, 7407, 7709, 7711, 7995
Kpn2I TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
Ksp22I TGATCA 6 cut(s) 3628, 3931, 5065, 5623, 7729, 8767
LguI GCTCTTC 3 cut(s) 177, 1118, 5892
LmnI GCTCC 9 cut(s) 2684, 3211, 4295, 5092, 5967, 7285, 7513, 7598, 8749
MfeI CAATTG 8 cut(s) 225, 594, 723, 994, 2316, 3935, 5627, 8299
MflI RGATCY 7 cut(s) 1289, 2186, 2656, 3362, 4419, 5182, 7797
MhlI GDGCHC 9 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 6552, 7856, 8901
MlsI TGGCCA 2 cut(s) 1584, 7033
MluNI TGGCCA 2 cut(s) 1584, 7033
Mox20I TGGCCA 2 cut(s) 1584, 7033
Mph1103I ATGCAT 4 cut(s) 1563, 4061, 5810, 7652
MroI TCCGGA 7 cut(s) 494, 1119, 2236, 2270, 7282, 7513, 7670
MroXI GAANNNNTTC 8 cut(s) 1127, 2796, 2893, 4070, 5117, 5448, 6755, 8148
MscI TGGCCA 2 cut(s) 1584, 7033
MslI CAYNNNNRTG 8 cut(s) 2593, 3354, 5073, 6209, 7044, 7940, 7968, 8763
Msp20I TGGCCA 2 cut(s) 1584, 7033
MspA1I CMGCKG 2 cut(s) 2384, 3637
MspCI CTTAAG 3 cut(s) 1370, 1705, 6383
MssI GTTTAAAC 1 cut(s) 5740
MunI CAATTG 8 cut(s) 225, 594, 723, 994, 2316, 3935, 5627, 8299
Mva1269I GAATGC 3 cut(s) 320, 587, 5810
MvnI CGCG 2 cut(s) 843, 7711
NciI CCSGG 3 cut(s) 3110, 3111, 6183
NcoI CCATGG 4 cut(s) 9, 2601, 8547, 8815
NdeI CATATG 2 cut(s) 3298, 7941
NheI GCTAGC 1 cut(s) 964
NmeAIII GCCGAG 1 cut(s) 3227
NmuCI GTSAC 7 cut(s) 937, 3104, 3505, 5168, 6806, 8054, 8291
NruI TCGCGA 1 cut(s) 843
NsiI ATGCAT 4 cut(s) 1563, 4061, 5810, 7652
NspI RCATGY 8 cut(s) 149, 1431, 2598, 3653, 4696, 5389, 5528, 7650
NspV TTCGAA 2 cut(s) 742, 1600
PaeI GCATGC 1 cut(s) 1431
PaeR7I CTCGAG 3 cut(s) 188, 5108, 7948
PagI TCATGA 3 cut(s) 3310, 3349, 8758
PasI CCCWGGG 2 cut(s) 884, 7916
PauI GCGCGC 1 cut(s) 7709
PceI AGGCCT 1 cut(s) 6626
PciI ACATGT 3 cut(s) 145, 3649, 5524
PciSI GCTCTTC 3 cut(s) 177, 1118, 5892
PcsI WCGNNNNNNNCGW 2 cut(s) 3427, 5484
PctI GAATGC 3 cut(s) 320, 587, 5810
PdmI GAANNNNTTC 8 cut(s) 1127, 2796, 2893, 4070, 5117, 5448, 6755, 8148
PflFI GACNNNGTC 1 cut(s) 5345
PflMI CCANNNNNTGG 5 cut(s) 1591, 5242, 6880, 7040, 8284
PmaCI CACGTG 1 cut(s) 7706
PmeI GTTTAAAC 1 cut(s) 5740
PmlI CACGTG 1 cut(s) 7706
Ppu21I YACGTR 7 cut(s) 800, 1394, 4321, 4525, 5040, 7706, 8259
PpuMI RGGWCCY 2 cut(s) 2545, 3305
PscI ACATGT 3 cut(s) 145, 3649, 5524
PshBI ATTAAT 4 cut(s) 989, 4785, 5309, 7503
PsiI TTATAA 5 cut(s) 866, 1025, 3048, 3443, 8367
Psp124BI GAGCTC 2 cut(s) 189, 5089
Psp1406I AACGTT 1 cut(s) 6400
Psp5II RGGWCCY 2 cut(s) 2545, 3305
PspCI CACGTG 1 cut(s) 7706
PspFI CCCAGC 3 cut(s) 4359, 5031, 8596
PspPI GGNCC 7 cut(s) 1987, 2545, 3305, 3670, 4652, 7055, 7145
PspPPI RGGWCCY 2 cut(s) 2545, 3305
PspXI VCTCGAGB 1 cut(s) 7948
PsrI GAACNNNNNNTAC 2 cut(s) 8133, 8165
PstI CTGCAG 3 cut(s) 1914, 2180, 4498
PsuI RGATCY 7 cut(s) 1289, 2186, 2656, 3362, 4419, 5182, 7797
PsyI GACNNNGTC 1 cut(s) 5345
PteI GCGCGC 1 cut(s) 7709
PvuII CAGCTG 1 cut(s) 2384
RruI TCGCGA 1 cut(s) 843
RseI CAYNNNNRTG 8 cut(s) 2593, 3354, 5073, 6209, 7044, 7940, 7968, 8763
SacI GAGCTC 2 cut(s) 189, 5089
SapI GCTCTTC 3 cut(s) 177, 1118, 5892
Sau96I GGNCC 7 cut(s) 1987, 2545, 3305, 3670, 4652, 7055, 7145
ScaI AGTACT 2 cut(s) 5437, 7276
SduI GDGCHC 9 cut(s) 189, 1113, 2325, 4300, 5089, 6204, 6552, 7856, 8901
SfcI CTRYAG 6 cut(s) 1910, 2176, 2928, 3855, 4494, 6845
Sfr274I CTCGAG 3 cut(s) 188, 5108, 7948
SfuI TTCGAA 2 cut(s) 742, 1600
SinI GGWCC 5 cut(s) 1987, 2545, 3305, 4652, 7055
SlaI CTCGAG 3 cut(s) 188, 5108, 7948
SmaI CCCGGG 1 cut(s) 3111
SmiMI CAYNNNNRTG 8 cut(s) 2593, 3354, 5073, 6209, 7044, 7940, 7968, 8763
SnaBI TACGTA 1 cut(s) 8259
SpeI ACTAGT 2 cut(s) 4235, 7220
SphI GCATGC 1 cut(s) 1431
SseBI AGGCCT 1 cut(s) 6626
SspI AATATT 3 cut(s) 5457, 7081, 8117
SstI GAGCTC 2 cut(s) 189, 5089
StuI AGGCCT 1 cut(s) 6626
StyI CCWWGG 8 cut(s) 9, 606, 2601, 5903, 6298, 8356, 8547, 8815
TatI WGTACW 6 cut(s) 1157, 5435, 7064, 7274, 7630, 8335
TauI GCSGC 5 cut(s) 1485, 2587, 3013, 4274, 4586
TseFI GTSAC 7 cut(s) 937, 3104, 3505, 5168, 6806, 8054, 8291
Tsp45I GTSAC 7 cut(s) 937, 3104, 3505, 5168, 6806, 8054, 8291
TspGWI ACGGA 8 cut(s) 2884, 3092, 3524, 4412, 6893, 7343, 7790, 8128
TspMI CCCGGG 1 cut(s) 3109
Tth111I GACNNNGTC 1 cut(s) 5345
Van91I CCANNNNNTGG 5 cut(s) 1591, 5242, 6880, 7040, 8284
Vha464I CTTAAG 3 cut(s) 1370, 1705, 6383
VpaK11BI GGWCC 5 cut(s) 1987, 2545, 3305, 4652, 7055
VspI ATTAAT 4 cut(s) 989, 4785, 5309, 7503
XagI CCTNNNNNAGG 1 cut(s) 4856
XbaI TCTAGA 2 cut(s) 3145, 4123
XceI RCATGY 8 cut(s) 149, 1431, 2598, 3653, 4696, 5389, 5528, 7650
XcmI CCANNNNNNNNNTGG 1 cut(s) 7421
XhoI CTCGAG 3 cut(s) 188, 5108, 7948
XmaI CCCGGG 1 cut(s) 3109
XmiI GTMKAC 6 cut(s) 2851, 4232, 4431, 6087, 7809, 8713
XmnI GAANNNNTTC 8 cut(s) 1127, 2796, 2893, 4070, 5117, 5448, 6755, 8148
ZraI GACGTC 1 cut(s) 4999
ZrmI AGTACT 2 cut(s) 5437, 7276
Zsp2I ATGCAT 4 cut(s) 1563, 4061, 5810, 7652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.