RLG00000028745

Pentatricopeptide repeat-containing protein At2g44880-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008546
Physical Location & Seq
Forward (+)
27365347 .. 27368692
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028745

Sequence Viewer

Length: 2271 bp
ATGAAAGACCAACTATGGAGATGGAGCTCCTTGGAAAGAAAATGCTTATACCTTCTCCAACAAGCCAACACAACACCACCCTCTCTTCTCCAAATCCATGCCTTCATCCTCCGAAACGCCCTCGAAACCAACCTCAATCTCGTCACCAAATTCATCACCACCTGCTCCTCCTCCTCCTCCTCCCCCTCGCACCTGATCAAACACGCCCGCCGCGTCTTCGATCACCAACCCAATAAGCACGACACGTTTCTCTGCAATGCCGTGATCAGAGCCCACATGGCCCAATTCGCCGAGTCTTTCGCTCTTTATAGAGATCTTAGACAGGACAAGGGTTTCGAGCCGGATGGGTACACATTCACAGCTCTGGCCAAGTCGTGCGGGTTTGATGGGGATAGATCAGAAGGAGAAGAGATTCATTGTCAGGCTGTTAAGACTGGGCTGTCCTTGGATTTGTATGTTTCGACGTCTTTGGTTGACATGTACGTGAAGTTTGGGACGATGGGTTGTGCAAGGAAGATGTTTGATGAAATGACTGAGAGAAATATAGTGTCGTGGACGGCTCTTGTTTGCGGGTATGCGAGGACGGGAGATATGGGCAGTGCGAGGAGGCTTTTTGATGAAATGCCTGAGAGGGACTCAGCCGCGTTTAATGCGTTGATTGATGGATATGTCAAGTTGGGGGAGATGGGCTCAGCTCGAAGTTTGTTTGATGAGATGAGGGATAGGAATGTGGTGACTTGGACTAGTATGATATACGGTTACTGCCATCGAGGTGATGTAGAGGCCGCTAAATCACTCTTTGATTCTATGCCCAAAAAGAATCTTATTTCTTGGAATGTGATGATTGGTGGTTATTGCCAAAACAAACGTCCCCATGAAGCCATGAGATTGTTTCATGAAATGCAGTCAACTACATCGCTTGAACCAGATGCTGTAACAGTTGTGAGCATTCTTCCGGCTATAGCTGATTTGGGTGCTTTGGATTTAGGGCATTGGGTTCACGAGTTTGTACAGAGAAAGAAGCTTGATAAGCTAACCAATATATGCACTGCGCTTGTGGATATGTTTGCAAAATGTGGTGAAATAAAAAAGGCCAAAAAACTCTTTAATGAGATGCCTGAAAAAGAAACGGCTTCTTGGAATGCTTTGATAAATGGGTTTGCAGTCAATGGCTATGGAAAGGAGGCACTAGAGGTATTTTTAGAGATGCAACGAGAAAAGTTTAAGCCTAACAATATAACCTTTGTCAGTGTTCTGTCCGCTTGTAACCATTGTGGTCTGGTAGAGGAAGGGAAATTTTGGTTTAGGAAAATGGAAAACTTTGGGCTCATTCCACAGATTGAGCATTATGGTTGTATGGTAGATCTTTTAGGAAGGGCAGGGTGCTTGGAGGAAGCTGAGAAGCTGATCAAGAGCATGCCTTACGATGTTAATGGGATAATTTTGAGTTCCTTTCTTTTTGCATGTGGGTATTGTGAGGATGTCACAAGAGCCAACAAAACTTTAGAGCAGGCTGTCAAACTGGAGCCATGGAATTATGGAAATTATGTTATGTTGAGAAATTTGTATGCGAGAAACAGAAGGTGGAGTGATACAGATAATATTAAGAGTTCGATGCGGAAGAATCAAACAGATAAAGAGGTCGGTTATAGTGTTATAGAGGTTGATGGTAGAATCAAGCAGTTTGTGGCTGGGGACAGGATGTACACATCTTCGGAAGCTATACATTTGACCTTGCTACAGAGTTGGAAGCACATGATGGGACAAGTCCCATGTTCTATGGCTAAGGTAATGGGGTTGATAATGAGTTCCATGTCCCTAGGGTCCGCAAAGATGATCAATTACGCAATGGAACCGGTGTACGACAAGTTTATACAGAATCCAGTTACAGATTTTGACGAGTTTCATGCTGCCATGCTTGGTGTTTGCAGCGCTTTAAATGCTGCTATGCCCGGTAAACACTACAATGTGCCCGGACTGGAGGAAGTAAAGGAATTTCACAGTAAATGGAACAACTTCTCGGCTCAAGAAAAGAGGGAGACATTCATCCAGTTTATGAAGGATAATGTGAAAGTTAACCAGGCTGATACCGCTACCTTGTTGACAGGAGTACTAGCGCCTCCAGGGGCCATGGCTGTTAAAAAAGCTGGGGAGAGTCTTCCCCAGCTGAAAATGATCAAGAACGTCCCAGACATCCTTTTCGTGCCGTCGGCAACAGTAGCAGTACTCATTACTGTAAGGTTCTCCAAAAGACTTTTCATGCGAAACTAG

Protein Analysis

757

Amino Acids

85.44

Weight (kDa)

8.13

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 183 - 211 3.6e-06 PPR repeat
PPR_2 PF13041 212 - 244 8.3e-07 PPR repeat family
PPR PF01535 215 - 244 3.9e-07 PPR repeat
PPR_2 PF13041 242 - 275 8.8e-09 PPR repeat family
PPR_1 PF12854 243 - 270 2.7e-07 PPR repeat
PPR PF01535 245 - 274 1.7e-08 PPR repeat
PPR_2 PF13041 274 - 318 1.7e-10 PPR repeat family
PPR_1 PF12854 274 - 302 9.8e-07 PPR repeat
PPR PF01535 276 - 303 6.5e-09 PPR repeat
PPR_2 PF13041 377 - 422 6.5e-11 PPR repeat family
PPR PF01535 379 - 407 1.2e-07 PPR repeat
PPR_3 PF13812 380 - 422 6.5e-06 Pentatricopeptide repeat domain
E_motif PF20431 494 - 554 2.1e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 170
AccII CGCG 2 cut(s) 213, 644
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 4 cut(s) 149, 1295, 1561, 1994
AcyI GRCGYC 1 cut(s) 464
AfaI GTAC 7 cut(s) 350, 482, 1011, 1706, 1862, 2112, 2226
AfeI AGCGCT 1 cut(s) 1933
AflIII ACRYGT 2 cut(s) 243, 477
AgeI ACCGGT 1 cut(s) 1855
AgsI TTSAA 1 cut(s) 923
AhlI ACTAGT 1 cut(s) 743
AjnI CCWGG 2 cut(s) 2079, 2122
AjuI GAANNNNNNNTTGG 1 cut(s) 2240
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 1 cut(s) 2033
AlwNI CAGNNNCTG 1 cut(s) 932
Aor51HI AGCGCT 1 cut(s) 1933
AoxI GGCC 5 cut(s) 279, 366, 783, 1092, 2127
ApeKI GCWGC 3 cut(s) 1910, 1929, 1943
ApoI RAATTY 4 cut(s) 149, 1295, 1561, 1994
ArsI GACNNNNNNTTYG 6 cut(s) 317, 349, 853, 885, 2183, 2215
AsiGI ACCGGT 1 cut(s) 1855
Asp700I GAANNNNTTC 2 cut(s) 411, 2015
AspA2I CCTAGG 1 cut(s) 1819
AspLEI GCGC 3 cut(s) 1054, 1934, 2119
AspS9I GGNCC 3 cut(s) 280, 1824, 2127
AsuC2I CCSGG 2 cut(s) 1953, 1974
AsuHPI GGTGA 6 cut(s) 136, 148, 215, 745, 785, 1091
AvaII GGWCC 1 cut(s) 1824
AvrII CCTAGG 1 cut(s) 1819
BaeGI GKGCMC 1 cut(s) 1974
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 4 cut(s) 29, 274, 692, 1329
BauI CACGAG 1 cut(s) 1001
BbsI GAAGAC 2 cut(s) 208, 2150
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 3 cut(s) 1897, 1930, 1941
BccI CCATC 9 cut(s) 15, 338, 380, 493, 656, 679, 774, 1661, 1753
BceAI ACGGC 4 cut(s) 245, 573, 1146, 2191
BcgI CGANNNNNNTGC 2 cut(s) 1889, 1923
BciT130I CCWGG 2 cut(s) 2081, 2124
BclI TGATCA 5 cut(s) 195, 264, 1407, 1836, 2175
BcnI CCSGG 2 cut(s) 1953, 1974
BcoDI GTCTC 1 cut(s) 2033
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 5 cut(s) 744, 1190, 1820, 2114, 2269
BfmI CTRYAG 2 cut(s) 960, 1739
BfoI RGCGCY 2 cut(s) 1935, 2120
BfuAI ACCTGC 1 cut(s) 170
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 2 cut(s) 313, 1363
BisI GCNGC 6 cut(s) 211, 642, 786, 1911, 1930, 1944
BlnI CCTAGG 1 cut(s) 1819
BlpI GCTNAGC 1 cut(s) 691
BlsI GCNGC 6 cut(s) 212, 643, 787, 1912, 1931, 1945
BmcAI AGTACT 2 cut(s) 2112, 2226
Bme1390I CCNGG 4 cut(s) 1953, 1974, 2081, 2124
Bme18I GGWCC 1 cut(s) 1824
BmgT120I GGNCC 3 cut(s) 280, 1824, 2127
BmiI GGNNCC 4 cut(s) 1527, 1825, 1854, 2128
BmrFI CCNGG 4 cut(s) 1953, 1974, 2081, 2124
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 919, 1104, 1197, 1605
BmuI ACTGGG 1 cut(s) 444
BpiI GAAGAC 2 cut(s) 208, 2150
BplI GAGNNNNNCTC 4 cut(s) 620, 652, 674, 706
BpmI CTGGAG 3 cut(s) 1544, 2000, 2106
Bpu10I CCTNAGC 1 cut(s) 1785
Bpu1102I GCTNAGC 1 cut(s) 691
BpuEI CTTGAG 1 cut(s) 2010
BpuMI CCSGG 2 cut(s) 1953, 1974
BsaAI YACGTR 1 cut(s) 484
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 6 cut(s) 30, 444, 1529, 1819, 2123, 2130
BsaWI WCCGGW 1 cut(s) 1855
Bse118I RCCGGY 1 cut(s) 1855
Bse1I ACTGG 5 cut(s) 439, 1527, 1883, 1983, 2050
Bse3DI GCAATG 2 cut(s) 262, 1854
BseBI CCWGG 2 cut(s) 2081, 2124
BseDI CCNNGG 6 cut(s) 30, 444, 1529, 1819, 2123, 2130
BseGI GGATG 6 cut(s) 105, 349, 1486, 1707, 2046, 2193
BseMI GCAATG 2 cut(s) 262, 1854
BseMII CTCAG 5 cut(s) 525, 618, 651, 705, 1389
BseNI ACTGG 5 cut(s) 439, 1527, 1883, 1983, 2050
BseRI GAGGAG 6 cut(s) 157, 160, 163, 166, 169, 619
BseSI GKGCMC 1 cut(s) 1974
BseXI GCAGC 3 cut(s) 1897, 1930, 1941
BseYI CCCAGC 3 cut(s) 1691, 2147, 2163
Bsh1236I CGCG 2 cut(s) 213, 644
BshFI GGCC 5 cut(s) 281, 368, 785, 1094, 2129
BshTI ACCGGT 1 cut(s) 1855
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 5 cut(s) 341, 956, 1856, 1953, 1974
BslFI GGGAC 8 cut(s) 508, 647, 855, 1709, 1754, 1776, 1801, 2171
BsmAI GTCTC 1 cut(s) 2033
BsmFI GGGAC 8 cut(s) 508, 647, 855, 1709, 1754, 1776, 1801, 2171
BsmI GAATGC 2 cut(s) 948, 1147
BsnI GGCC 5 cut(s) 281, 368, 785, 1094, 2129
Bsp1286I GDGCHC 5 cut(s) 29, 274, 692, 1329, 1974
Bsp1407I TGTACA 2 cut(s) 1009, 1704
Bsp143I GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
Bsp1720I GCTNAGC 1 cut(s) 691
Bsp19I CCATGG 2 cut(s) 1529, 2130
BspANI GGCC 5 cut(s) 281, 368, 785, 1094, 2129
BspCNI CTCAG 5 cut(s) 526, 619, 650, 704, 1390
BspFNI CGCG 2 cut(s) 213, 644
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 1527, 1825, 1854, 2128
BspMI ACCTGC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 262, 1854
BsrFI RCCGGY 1 cut(s) 1855
BsrGI TGTACA 2 cut(s) 1009, 1704
BsrI ACTGG 5 cut(s) 439, 1527, 1883, 1983, 2050
BssAI RCCGGY 1 cut(s) 1855
BssECI CCNNGG 6 cut(s) 30, 444, 1529, 1819, 2123, 2130
BssMI GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
BssNI GRCGYC 1 cut(s) 464
BssSI CACGAG 1 cut(s) 1001
BssT1I CCWWGG 5 cut(s) 30, 444, 1529, 1819, 2130
Bst2BI CACGAG 1 cut(s) 1001
Bst2UI CCWGG 2 cut(s) 2081, 2124
Bst4CI ACNGT 5 cut(s) 758, 940, 2003, 2218, 2236
Bst6I CTCTTC 2 cut(s) 90, 402
BstACI GRCGYC 1 cut(s) 464
BstAUI TGTACA 2 cut(s) 1009, 1704
BstBAI YACGTR 1 cut(s) 484
BstC8I GCNNGC 3 cut(s) 208, 1418, 1512
BstDEI CTNAG 7 cut(s) 317, 534, 627, 637, 691, 1398, 1785
BstDSI CCRYGG 2 cut(s) 1529, 2130
BstF5I GGATG 6 cut(s) 105, 349, 1486, 1707, 2046, 2193
BstFNI CGCG 2 cut(s) 213, 644
BstH2I RGCGCY 2 cut(s) 1935, 2120
BstHHI GCGC 3 cut(s) 1054, 1934, 2119
BstKTI GATC 9 cut(s) 198, 223, 267, 316, 398, 1366, 1410, 1839, 2178
BstMAI GTCTC 1 cut(s) 2033
BstMBI GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
BstMWI GCNNNNNNNGC 7 cut(s) 278, 287, 650, 1030, 1940, 2090, 2219
BstNI CCWGG 2 cut(s) 2081, 2124
BstNSI RCATGY 3 cut(s) 481, 1420, 1467
BstSCI CCNGG 4 cut(s) 1951, 1972, 2079, 2122
BstSFI CTRYAG 2 cut(s) 960, 1739
BstSLI GKGCMC 1 cut(s) 1974
BstUI CGCG 2 cut(s) 213, 644
BstV1I GCAGC 3 cut(s) 1897, 1930, 1941
BstV2I GAAGAC 2 cut(s) 208, 2150
BstX2I RGATCY 2 cut(s) 313, 1363
BstYI RGATCY 2 cut(s) 313, 1363
BsuRI GGCC 5 cut(s) 281, 368, 785, 1094, 2129
BtgI CCRYGG 2 cut(s) 1529, 2130
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 6 cut(s) 105, 349, 1486, 1707, 2046, 2193
BtsI GCAGTG 2 cut(s) 604, 1047
BtsIMutI CAGTG 3 cut(s) 604, 1047, 1255
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 3 cut(s) 208, 1418, 1512
CaiI CAGNNNCTG 1 cut(s) 932
CciI TCATGA 1 cut(s) 895
CfoI GCGC 3 cut(s) 1054, 1934, 2119
Cfr10I RCCGGY 1 cut(s) 1855
Cfr13I GGNCC 3 cut(s) 280, 1824, 2127
CseI GACGC 1 cut(s) 202
Csp6I GTAC 7 cut(s) 349, 481, 1010, 1705, 1861, 2111, 2225
CspAI ACCGGT 1 cut(s) 1855
CviQI GTAC 7 cut(s) 349, 481, 1010, 1705, 1861, 2111, 2225
DdeI CTNAG 7 cut(s) 317, 534, 627, 637, 691, 1398, 1785
DpnI GATC 9 cut(s) 197, 222, 266, 315, 397, 1365, 1409, 1838, 2177
DpnII GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
DraI TTTAAA 1 cut(s) 1938
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 90, 402
EarI CTCTTC 2 cut(s) 90, 402
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 5 cut(s) 30, 444, 1529, 1819, 2130
Eco24I GRGCYC 4 cut(s) 29, 274, 692, 1329
Eco47I GGWCC 1 cut(s) 1824
Eco47III AGCGCT 1 cut(s) 1933
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 2079, 2122
EcoT14I CCWWGG 5 cut(s) 30, 444, 1529, 1819, 2130
EcoT38I GRGCYC 4 cut(s) 29, 274, 692, 1329
ErhI CCWWGG 5 cut(s) 30, 444, 1529, 1819, 2130
FaqI GGGAC 8 cut(s) 508, 647, 855, 1709, 1754, 1776, 1801, 2171
FauI CCCGC 3 cut(s) 215, 371, 563
FbaI TGATCA 5 cut(s) 195, 264, 1407, 1836, 2175
Fnu4HI GCNGC 6 cut(s) 211, 642, 786, 1911, 1930, 1944
FokI GGATG 6 cut(s) 92, 356, 1493, 1714, 2033, 2180
FriOI GRGCYC 4 cut(s) 29, 274, 692, 1329
Fsp4HI GCNGC 6 cut(s) 211, 642, 786, 1911, 1930, 1944
FspBI CTAG 5 cut(s) 744, 1190, 1820, 2114, 2269
GlaI GCGC 3 cut(s) 1053, 1933, 2118
GluI GCNGC 6 cut(s) 211, 642, 786, 1911, 1930, 1944
GsaI CCCAGC 3 cut(s) 1695, 2151, 2167
GsuI CTGGAG 3 cut(s) 1544, 2000, 2106
HaeII RGCGCY 2 cut(s) 1935, 2120
HaeIII GGCC 5 cut(s) 281, 368, 785, 1094, 2129
HapII CCGG 5 cut(s) 341, 956, 1856, 1953, 1974
HgaI GACGC 1 cut(s) 202
HhaI GCGC 3 cut(s) 1054, 1934, 2119
Hin1I GRCGYC 1 cut(s) 464
Hin6I GCGC 3 cut(s) 1052, 1932, 2117
HinP1I GCGC 3 cut(s) 1052, 1932, 2117
HincII GTYRAC 4 cut(s) 475, 909, 2077, 2103
HindII GTYRAC 4 cut(s) 475, 909, 2077, 2103
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 9 cut(s) 293, 412, 635, 803, 820, 1624, 1674, 1879, 2155
HpaI GTTAAC 1 cut(s) 2077
HpaII CCGG 5 cut(s) 341, 956, 1856, 1953, 1974
HphI GGTGA 6 cut(s) 136, 148, 215, 745, 785, 1091
Hpy188I TCNGA 4 cut(s) 113, 269, 400, 1717
Hpy188III TCNNGA 5 cut(s) 896, 1001, 1411, 2027, 2179
Hpy99I CGWCG 2 cut(s) 466, 2212
HpyAV CCTTC 7 cut(s) 62, 112, 395, 1283, 1368, 1575, 2053
HpyCH4III ACNGT 5 cut(s) 758, 940, 2003, 2218, 2236
HpyCH4IV ACGT 5 cut(s) 245, 464, 483, 868, 2184
HpyCH4V TGCA 9 cut(s) 255, 509, 904, 1047, 1070, 1163, 1210, 1463, 1929
HpyF10VI GCNNNNNNNGC 7 cut(s) 278, 287, 650, 1030, 1940, 2090, 2219
HpyF3I CTNAG 7 cut(s) 317, 534, 627, 637, 691, 1398, 1785
HpySE526I ACGT 5 cut(s) 245, 464, 483, 868, 2184
Hsp92I GRCGYC 1 cut(s) 464
HspAI GCGC 3 cut(s) 1052, 1932, 2117
Ksp22I TGATCA 5 cut(s) 195, 264, 1407, 1836, 2175
KspAI GTTAAC 1 cut(s) 2077
Kzo9I GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
LmnI GCTCC 4 cut(s) 24, 32, 170, 1525
Lsp1109I GCAGC 3 cut(s) 1897, 1930, 1941
LweI GCATC 4 cut(s) 919, 1104, 1197, 1605
MaeI CTAG 5 cut(s) 744, 1190, 1820, 2114, 2269
MaeII ACGT 5 cut(s) 245, 464, 483, 868, 2184
MaeIII GTNAC 7 cut(s) 142, 733, 758, 934, 1265, 1483, 1885
MalI GATC 9 cut(s) 197, 222, 266, 315, 397, 1365, 1409, 1838, 2177
MboI GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
MboII GAAGA 8 cut(s) 77, 208, 419, 526, 944, 1633, 1704, 2150
MflI RGATCY 2 cut(s) 313, 1363
MhlI GDGCHC 5 cut(s) 29, 274, 692, 1329, 1974
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 9 cut(s) 149, 284, 1295, 1440, 1534, 1543, 1561, 1840, 1994
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 3 cut(s) 302, 629, 2164
MmeI TCCRAC 2 cut(s) 82, 1727
Mox20I TGGCCA 1 cut(s) 368
MroXI GAANNNNTTC 2 cut(s) 411, 2015
MscI TGGCCA 1 cut(s) 368
MseI TTAA 9 cut(s) 429, 648, 1107, 1224, 1431, 1605, 1937, 2076, 2139
MslI CAYNNNNRTG 3 cut(s) 482, 771, 1965
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 2167
MspI CCGG 5 cut(s) 341, 956, 1856, 1953, 1974
MspR9I CCNGG 4 cut(s) 1953, 1974, 2081, 2124
Mva1269I GAATGC 2 cut(s) 948, 1147
MvaI CCWGG 2 cut(s) 2081, 2124
MvnI CGCG 2 cut(s) 213, 644
MwoI GCNNNNNNNGC 7 cut(s) 278, 287, 650, 1030, 1940, 2090, 2219
NciI CCSGG 2 cut(s) 1953, 1974
NcoI CCATGG 2 cut(s) 1529, 2130
NdeII GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
NlaIV GGNNCC 4 cut(s) 1527, 1825, 1854, 2128
NmeAIII GCCGAG 2 cut(s) 316, 2000
NmuCI GTSAC 3 cut(s) 142, 733, 1483
NspI RCATGY 3 cut(s) 481, 1420, 1467
PaeI GCATGC 1 cut(s) 1420
PagI TCATGA 1 cut(s) 895
PaqCI CACCTGC 1 cut(s) 170
PciI ACATGT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 2 cut(s) 948, 1147
PdmI GAANNNNTTC 2 cut(s) 411, 2015
PfeI GAWTC 6 cut(s) 412, 803, 820, 1624, 1674, 1879
PinAI ACCGGT 1 cut(s) 1855
PkrI GCNGC 6 cut(s) 212, 643, 787, 1912, 1931, 1945
PleI GAGTC 3 cut(s) 301, 629, 2163
PpsI GAGTC 3 cut(s) 301, 629, 2163
Ppu21I YACGTR 1 cut(s) 484
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 2079, 2122
PspFI CCCAGC 3 cut(s) 1691, 2147, 2163
PspGI CCWGG 2 cut(s) 2079, 2122
PspN4I GGNNCC 4 cut(s) 1527, 1825, 1854, 2128
PspPI GGNCC 3 cut(s) 280, 1824, 2127
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 2 cut(s) 313, 1363
PvuII CAGCTG 1 cut(s) 2167
RsaI GTAC 7 cut(s) 350, 482, 1011, 1706, 1862, 2112, 2226
RsaNI GTAC 7 cut(s) 349, 481, 1010, 1705, 1861, 2111, 2225
RseI CAYNNNNRTG 3 cut(s) 482, 771, 1965
SacI GAGCTC 1 cut(s) 29
SaqAI TTAA 9 cut(s) 429, 648, 1107, 1224, 1431, 1605, 1937, 2076, 2139
SatI GCNGC 6 cut(s) 211, 642, 786, 1911, 1930, 1944
Sau3AI GATC 9 cut(s) 195, 220, 264, 313, 395, 1363, 1407, 1836, 2175
Sau96I GGNCC 3 cut(s) 280, 1824, 2127
ScaI AGTACT 2 cut(s) 2112, 2226
SchI GAGTC 3 cut(s) 302, 629, 2164
ScrFI CCNGG 4 cut(s) 1953, 1974, 2081, 2124
SduI GDGCHC 5 cut(s) 29, 274, 692, 1329, 1974
SfaNI GCATC 4 cut(s) 919, 1104, 1197, 1605
SfcI CTRYAG 2 cut(s) 960, 1739
SinI GGWCC 1 cut(s) 1824
SmiMI CAYNNNNRTG 3 cut(s) 482, 771, 1965
SmlI CTYRAG 1 cut(s) 2025
SmoI CTYRAG 1 cut(s) 2025
SpeI ACTAGT 1 cut(s) 743
SphI GCATGC 1 cut(s) 1420
Sse9I AATT 9 cut(s) 149, 284, 1295, 1440, 1534, 1543, 1561, 1840, 1994
SspI AATATT 1 cut(s) 1603
SspMI CTAG 5 cut(s) 744, 1190, 1820, 2114, 2269
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 4 cut(s) 1951, 1972, 2079, 2122
StyI CCWWGG 5 cut(s) 30, 444, 1529, 1819, 2130
TaaI ACNGT 5 cut(s) 758, 940, 2003, 2218, 2236
TaiI ACGT 5 cut(s) 248, 467, 486, 871, 2187
TaqI TCGA 7 cut(s) 123, 219, 336, 461, 697, 769, 1613
TasI AATT 9 cut(s) 149, 284, 1295, 1440, 1534, 1543, 1561, 1840, 1994
TatI WGTACW 4 cut(s) 1009, 1704, 2110, 2224
TauI GCSGC 3 cut(s) 213, 644, 788
TfiI GAWTC 6 cut(s) 412, 803, 820, 1624, 1674, 1879
Tru1I TTAA 9 cut(s) 429, 648, 1107, 1224, 1431, 1605, 1937, 2076, 2139
Tru9I TTAA 9 cut(s) 429, 648, 1107, 1224, 1431, 1605, 1937, 2076, 2139
TscAI CASTG 3 cut(s) 604, 1054, 1255
TseFI GTSAC 3 cut(s) 142, 733, 1483
TseI GCWGC 3 cut(s) 1910, 1929, 1943
Tsp45I GTSAC 3 cut(s) 142, 733, 1483
TspRI CASTG 3 cut(s) 604, 1054, 1255
VpaK11BI GGWCC 1 cut(s) 1824
XapI RAATTY 4 cut(s) 149, 1295, 1561, 1994
XceI RCATGY 3 cut(s) 481, 1420, 1467
XmaJI CCTAGG 1 cut(s) 1819
XmnI GAANNNNTTC 2 cut(s) 411, 2015
XspI CTAG 5 cut(s) 744, 1190, 1820, 2114, 2269
ZraI GACGTC 1 cut(s) 465
ZrmI AGTACT 2 cut(s) 2112, 2226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.