AT2G45200

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011092
Physical Location & Seq
Reverse (-)
18637335 .. 18640039
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45200.2

Sequence Viewer

Length: 720 bp
ATGACAGAATCGAGTCTGGATCTGCAGGAATCTGGTTGGGAAGAACTTAGGCGGGAAGCTCGTAAAATTGAAGGAGATCTAGACGTTAAGCTCTCTTCTTACGCTAAGCTAGGCGCCAGGTTTACTCAAGGCGGGTATGTTGACACTGGGTCTCCAACCGTTGGATCGGGGAGATCATGGAAGTCTATGGAGATGGAAATTCAATCGTTGCTTGAGAAGTTGTTGGATATTAATGATTCCATGAGTAGATGTGCTGCATCTGCTGCACCCACAACATCGGTTACTCAAAAACTAGCAAGGCACAGGGATATACTTCATGAATATACCCAGGAGTTTCGAAGAATAAAAGGAAATATAAACTCGTTGAGAGAACACGCTGAGCTTCTGAGTTCTGTCAGGGATGACATAAGTGAATATAAGGCTTCTGGTAGTATGTCACCAGGTGTGCAAGTGTTAAGGGAGAGAGCTTCAATCCATGGAAGTATATCCCATATTGATGATGTGATTGGTCAAGCTCAAGCGACAAGAGCAGTTCTTGGCTCTCAACGATCTCTGTTTTCAGATGTTCAAGGGAAAGTAAAAAATCTCGGAGACAAATTCCCAGTGATTCGTGGCTTACTTGGCTCAATTAAAAGGAAACGTTCTCGGGACACACTTATCCTCTCCGCTGTCATCGCTGCTTGTACGTTGTTTCTAATCATCTACTGGCTCTCAAAATAA

Protein Analysis

239

Amino Acids

26.47

Weight (kDa)

9.22

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 168 - 232 7.2e-18 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45200 AT2G45200
fragaria_vesca FvH4_7g11270
malus_domestica MD02G1205800.v1.1 MD07G1117300.v1.1
prunus_persica Prupe.2G140500_v2.0.a1 Prupe.2G140500_v2.0.a1
pyrus_communis pycom02g17040 pycom07g10740
rosa_chinensis RchiOBHm_Chr1g0349411
rosa_laevigata RLG00000028551
rosa_multiflora Rmu_sc0002495.1_g000004
rosa_roxburghii Rroxscaffold_4G00305560
rosa_rugosa Rorug01G0203500 Rorug01G0203600.1
rosa_samantha Rh1AG220900 Rh1BG187000 Rh1CG205900 Rh1DG216300
rosa_wichuraiana Rw1G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 113
AccB7I CCANNNNNTGG 1 cut(s) 161
AciI CCGC 3 cut(s) 52, 132, 666
AclI AACGTT 1 cut(s) 640
AclWI GGATC 2 cut(s) 27, 172
AcsI RAATTY 2 cut(s) 198, 596
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 443
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 4 cut(s) 71, 203, 471, 569
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 3 cut(s) 116, 327, 439
AluBI AGCT 6 cut(s) 59, 91, 109, 382, 467, 515
AluI AGCT 6 cut(s) 59, 91, 109, 382, 467, 515
Alw26I GTCTC 2 cut(s) 156, 585
AlwI GGATC 2 cut(s) 27, 172
Ama87I CYCGRG 1 cut(s) 645
ApeKI GCWGC 3 cut(s) 254, 263, 677
ApoI RAATTY 2 cut(s) 198, 596
AseI ATTAAT 1 cut(s) 231
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 429
AsuII TTCGAA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 645
BanI GGYRCC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 241, 250, 664
BccI CCATC 1 cut(s) 187
BciT130I CCWGG 3 cut(s) 118, 329, 441
BcoDI GTCTC 2 cut(s) 156, 585
BfaI CTAG 3 cut(s) 80, 110, 293
BfmI CTRYAG 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 117
BglII AGATCT 1 cut(s) 76
BisI GCNGC 3 cut(s) 255, 264, 678
BlpI GCTNAGC 2 cut(s) 105, 378
BlsI GCNGC 3 cut(s) 256, 265, 679
Bme1390I CCNGG 3 cut(s) 118, 329, 441
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 645
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 3 cut(s) 118, 329, 441
BmrI ACTGGG 2 cut(s) 156, 596
BmsI GCATC 1 cut(s) 266
BmuI ACTGGG 2 cut(s) 156, 596
Bpu1102I GCTNAGC 2 cut(s) 105, 378
Bpu14I TTCGAA 1 cut(s) 337
BpuEI CTTGAG 3 cut(s) 111, 233, 501
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 327, 475
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 3 cut(s) 151, 602, 710
BseBI CCWGG 3 cut(s) 118, 329, 441
BseDI CCNNGG 2 cut(s) 327, 475
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 2 cut(s) 369, 377
BseNI ACTGG 3 cut(s) 151, 602, 710
BseXI GCAGC 3 cut(s) 241, 250, 664
BsgI GTGCAG 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 645
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 156, 585
BsmFI GGGAC 1 cut(s) 662
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 645
Bsp119I TTCGAA 1 cut(s) 337
Bsp143I GATC 5 cut(s) 19, 76, 164, 173, 548
Bsp1720I GCTNAGC 2 cut(s) 105, 378
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 3 cut(s) 52, 132, 666
BspCNI CTCAG 2 cut(s) 370, 378
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 115
BspMAI CTGCAG 1 cut(s) 27
BspPI GGATC 2 cut(s) 27, 172
BspT104I TTCGAA 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 156
BsrI ACTGG 3 cut(s) 151, 602, 710
BssECI CCNNGG 2 cut(s) 327, 475
BssMI GATC 5 cut(s) 19, 76, 164, 173, 548
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 118, 329, 441
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 337
BstDEI CTNAG 4 cut(s) 47, 105, 378, 386
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 5 cut(s) 22, 79, 167, 176, 551
BstMAI GTCTC 2 cut(s) 156, 585
BstMBI GATC 5 cut(s) 19, 76, 164, 173, 548
BstMWI GCNNNNNNNGC 5 cut(s) 260, 263, 527, 621, 674
BstNI CCWGG 3 cut(s) 118, 329, 441
BstSCI CCNGG 3 cut(s) 116, 327, 439
BstSFI CTRYAG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 241, 250, 664
BstX2I RGATCY 2 cut(s) 19, 76
BstYI RGATCY 2 cut(s) 19, 76
BtgI CCRYGG 1 cut(s) 475
BtgZI GCGATG 1 cut(s) 658
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 144, 609
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 116
CsiI ACCWGGT 1 cut(s) 439
Csp6I GTAC 1 cut(s) 684
CviAII CATG 4 cut(s) 177, 241, 317, 476
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 4 cut(s) 47, 105, 378, 386
DinI GGCGCC 1 cut(s) 115
DpnI GATC 5 cut(s) 21, 78, 166, 175, 550
DpnII GATC 5 cut(s) 19, 76, 164, 173, 548
DraIII CACNNNGTG 1 cut(s) 443
DriI GACNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 475
Eco31I GGTCTC 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 645
EcoRII CCWGG 3 cut(s) 116, 327, 439
EcoT14I CCWWGG 1 cut(s) 475
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 4 cut(s) 180, 244, 320, 479
FaqI GGGAC 1 cut(s) 662
FatI CATG 4 cut(s) 176, 240, 316, 475
FauI CCCGC 2 cut(s) 45, 125
Fnu4HI GCNGC 3 cut(s) 255, 264, 678
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 3 cut(s) 255, 264, 678
FspBI CTAG 3 cut(s) 80, 110, 293
GlaI GCGC 1 cut(s) 115
GluI GCNGC 3 cut(s) 255, 264, 678
HaeII RGCGCY 1 cut(s) 117
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 180, 244, 320, 479
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 5 cut(s) 8, 13, 29, 236, 607
HphI GGTGA 1 cut(s) 429
Hpy166II GTNNAC 2 cut(s) 123, 142
Hpy188I TCNGA 3 cut(s) 387, 562, 590
Hpy188III TCNNGA 4 cut(s) 17, 80, 317, 647
Hpy8I GTNNAC 2 cut(s) 123, 142
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 3 cut(s) 84, 640, 686
HpyCH4V TGCA 4 cut(s) 25, 257, 266, 448
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 263, 527, 621, 674
HpyF3I CTNAG 4 cut(s) 47, 105, 378, 386
HpySE526I ACGT 3 cut(s) 84, 640, 686
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 180, 244, 320, 479
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
Kzo9I GATC 5 cut(s) 19, 76, 164, 173, 548
Lsp1109I GCAGC 3 cut(s) 241, 250, 664
LweI GCATC 1 cut(s) 266
MabI ACCWGGT 1 cut(s) 439
MaeI CTAG 3 cut(s) 80, 110, 293
MaeII ACGT 3 cut(s) 84, 640, 686
MaeIII GTNAC 2 cut(s) 280, 435
MalI GATC 5 cut(s) 21, 78, 166, 175, 550
MboI GATC 5 cut(s) 19, 76, 164, 173, 548
MboII GAAGA 3 cut(s) 53, 87, 351
MflI RGATCY 2 cut(s) 19, 76
MluCI AATT 4 cut(s) 66, 198, 596, 627
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 3 cut(s) 142, 179, 204
MnlI CCTC 1 cut(s) 671
MseI TTAA 4 cut(s) 87, 231, 455, 630
MslI CAYNNNNRTG 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 668
MspR9I CCNGG 3 cut(s) 118, 329, 441
MvaI CCWGG 3 cut(s) 118, 329, 441
MwoI GCNNNNNNNGC 5 cut(s) 260, 263, 527, 621, 674
NarI GGCGCC 1 cut(s) 114
NcoI CCATGG 1 cut(s) 475
NdeII GATC 5 cut(s) 19, 76, 164, 173, 548
NlaIII CATG 4 cut(s) 180, 244, 320, 479
NlaIV GGNNCC 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 435
NspV TTCGAA 1 cut(s) 337
PagI TCATGA 1 cut(s) 316
PfeI GAWTC 4 cut(s) 8, 29, 236, 607
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 3 cut(s) 256, 265, 679
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 21
PshBI ATTAAT 1 cut(s) 231
Psp1406I AACGTT 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 116, 327, 439
PspGI CCWGG 3 cut(s) 116, 327, 439
PspN4I GGNNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 27
PsuI RGATCY 2 cut(s) 19, 76
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
RseI CAYNNNNRTG 1 cut(s) 495
SaqAI TTAA 4 cut(s) 87, 231, 455, 630
SatI GCNGC 3 cut(s) 255, 264, 678
Sau3AI GATC 5 cut(s) 19, 76, 164, 173, 548
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 118, 329, 441
SexAI ACCWGGT 1 cut(s) 439
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 23
SfoI GGCGCC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 3 cut(s) 126, 212, 516
SmoI CTYRAG 3 cut(s) 126, 212, 516
Sse9I AATT 4 cut(s) 66, 198, 596, 627
SsiI CCGC 3 cut(s) 52, 132, 666
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 3 cut(s) 80, 110, 293
StyD4I CCNGG 3 cut(s) 116, 327, 439
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 3 cut(s) 87, 643, 689
TaqI TCGA 2 cut(s) 11, 337
TasI AATT 4 cut(s) 66, 198, 596, 627
TfiI GAWTC 4 cut(s) 8, 29, 236, 607
Tru1I TTAA 4 cut(s) 87, 231, 455, 630
Tru9I TTAA 4 cut(s) 87, 231, 455, 630
TscAI CASTG 2 cut(s) 151, 609
TseFI GTSAC 1 cut(s) 435
TseI GCWGC 3 cut(s) 254, 263, 677
Tsp45I GTSAC 1 cut(s) 435
TspDTI ATGAA 2 cut(s) 305, 333
TspRI CASTG 2 cut(s) 151, 609
Van91I CCANNNNNTGG 1 cut(s) 161
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 2 cut(s) 198, 596
XbaI TCTAGA 1 cut(s) 79
XspI CTAG 3 cut(s) 80, 110, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.