MD07G1117300.v1.1

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011092
Physical Location & Seq
Reverse (-)
14357697 .. 14370314
12618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1117300.v1.1.491

Sequence Viewer

Length: 720 bp
ATGACGGATTCGAATCTGGAGTTGCAGGAATCGGGTTGGGAGGAGCTGAGGAGGGAGGCCAGGAAGATTGAGGGAGATCTTGATGTGAAGCTCTCTTCTTACGCTAAGCTTGGTGCTAGGTTCACTCAAGGAGGTTATGTAGATTCTGGGTCACCACCTGTTAGTTCGAGCAGGTCATGGAAGTCCATGGAAATGGAGATCGAATCTTTGCTAGAGAAACTTTTGGATATCAATGATTCAATGAGTCGATGTGCTGCATCTGCTACACCAGCTACTTCTGTAACTCAGAAGCTAGCAAGGCATAGAGACATACTTCATGAGTTTACCCAGGAATTTAGAAGAATCAAGGGGAACATAAATTCATTGAGGGAACATGCGGAGCTTCTGACTTCAGTCAGGGATGATATCAGTGAGTACAAGGCATCGGGGAGTATGTCACCAAGAATGCAAATACTACGGGAGAGAGCTGCCATCCACGGAAGTGTATCTCATATAGATGAGGTGATTAGTCAAGCTCAAACAACAAGGGCAGTCTTGGGCTCTCAAAGGGCTCTGTTTGGAGATGTTCAAGGGAAAGTGAAAAATCTAAGTGACAAGTTCCCTATAATCCGCGGCCTACTTGGTTCGATTCGAAGGCGGCGATCAAGAGACACGCTTATTCTGTCTGCAGTCATTGCAGCTTGTACCTTGTTTCTTATTATCTACTGGCTTTCAAAATAA

Protein Analysis

240

Amino Acids

26.78

Weight (kDa)

9.29

Isoelectric Point (pI)

62.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 150 - 214 4.9e-16 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45200 AT2G45200
fragaria_vesca FvH4_7g11270
malus_domestica MD02G1205800.v1.1 MD07G1117300.v1.1
prunus_persica Prupe.2G140500_v2.0.a1 Prupe.2G140500_v2.0.a1
pyrus_communis pycom02g17040 pycom07g10740
rosa_chinensis RchiOBHm_Chr1g0349411
rosa_laevigata RLG00000028551
rosa_multiflora Rmu_sc0002495.1_g000004
rosa_roxburghii Rroxscaffold_4G00305560
rosa_rugosa Rorug01G0203500 Rorug01G0203600.1
rosa_samantha Rh1AG220900 Rh1BG187000 Rh1CG205900 Rh1DG216300
rosa_wichuraiana Rw1G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AccII CGCG 1 cut(s) 612
AciI CCGC 4 cut(s) 377, 610, 612, 637
AcsI RAATTY 2 cut(s) 332, 358
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 2 cut(s) 416, 685
AgsI TTSAA 3 cut(s) 240, 569, 714
AjnI CCWGG 2 cut(s) 59, 327
AleI CACNNNNGTG 1 cut(s) 480
AluBI AGCT 9 cut(s) 46, 91, 109, 272, 292, 382, 467, 515, 680
AluI AGCT 9 cut(s) 46, 91, 109, 272, 292, 382, 467, 515, 680
Alw26I GTCTC 2 cut(s) 300, 642
AoxI GGCC 2 cut(s) 57, 613
ApeKI GCWGC 3 cut(s) 254, 467, 677
ApoI RAATTY 2 cut(s) 332, 358
AsuHPI GGTGA 3 cut(s) 144, 429, 514
AsuII TTCGAA 2 cut(s) 11, 631
AsuNHI GCTAGC 1 cut(s) 292
BanII GRGCYC 2 cut(s) 542, 553
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 241, 454, 689
BccI CCATC 1 cut(s) 479
BciT130I CCWGG 2 cut(s) 61, 329
BcoDI GTCTC 2 cut(s) 300, 642
BfaI CTAG 3 cut(s) 117, 212, 293
BfmI CTRYAG 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 162
BglII AGATCT 1 cut(s) 76
BisI GCNGC 5 cut(s) 255, 468, 613, 638, 678
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 5 cut(s) 256, 469, 614, 639, 679
Bme1390I CCNGG 2 cut(s) 61, 329
BmrFI CCNGG 2 cut(s) 61, 329
BmsI GCATC 2 cut(s) 266, 431
BmtI GCTAGC 1 cut(s) 296
BoxI GACNNNNGTC 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 105
Bpu14I TTCGAA 2 cut(s) 11, 631
BpuEI CTTGAG 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 4 cut(s) 186, 327, 475, 610
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bse1I ACTGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 61, 329
BseDI CCNNGG 4 cut(s) 186, 327, 475, 610
BseGI GGATG 2 cut(s) 406, 471
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 2 cut(s) 38, 299
BseNI ACTGG 1 cut(s) 710
BseRI GAGGAG 2 cut(s) 56, 64
BseXI GCAGC 3 cut(s) 241, 454, 689
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 2 cut(s) 59, 615
BsmAI GTCTC 2 cut(s) 300, 642
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 2 cut(s) 59, 615
Bsp119I TTCGAA 2 cut(s) 11, 631
Bsp1286I GDGCHC 2 cut(s) 542, 553
Bsp143I GATC 3 cut(s) 76, 198, 641
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 377, 610, 612, 637
BspANI GGCC 2 cut(s) 59, 615
BspCNI CTCAG 2 cut(s) 39, 298
BspFNI CGCG 1 cut(s) 612
BspHI TCATGA 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 296
BspT104I TTCGAA 2 cut(s) 11, 631
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 1 cut(s) 710
BssECI CCNNGG 4 cut(s) 186, 327, 475, 610
BssMI GATC 3 cut(s) 76, 198, 641
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 61, 329
Bst6I CTCTTC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBI TTCGAA 2 cut(s) 11, 631
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 4 cut(s) 47, 105, 285, 587
BstDSI CCRYGG 3 cut(s) 186, 475, 610
BstEII GGTNACC 1 cut(s) 150
BstF5I GGATG 2 cut(s) 406, 471
BstFNI CGCG 1 cut(s) 612
BstKTI GATC 3 cut(s) 79, 201, 644
BstMAI GTCTC 2 cut(s) 300, 642
BstMBI GATC 3 cut(s) 76, 198, 641
BstMWI GCNNNNNNNGC 4 cut(s) 260, 269, 298, 674
BstNI CCWGG 2 cut(s) 61, 329
BstNSI RCATGY 1 cut(s) 377
BstPAI GACNNNNGTC 1 cut(s) 392
BstPI GGTNACC 1 cut(s) 150
BstSCI CCNGG 2 cut(s) 59, 327
BstSFI CTRYAG 1 cut(s) 666
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 241, 454, 689
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 59, 615
BtgI CCRYGG 3 cut(s) 186, 475, 610
BtsCI GGATG 2 cut(s) 406, 471
BtsIMutI CAGTG 1 cut(s) 415
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 294
CciI TCATGA 1 cut(s) 316
Cfr42I CCGCGG 1 cut(s) 613
Csp6I GTAC 2 cut(s) 415, 684
CviAII CATG 4 cut(s) 177, 187, 317, 374
CviQI GTAC 2 cut(s) 415, 684
DdeI CTNAG 4 cut(s) 47, 105, 285, 587
DpnI GATC 3 cut(s) 78, 200, 643
DpnII GATC 3 cut(s) 76, 198, 641
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 542, 553
Eco32I GATATC 2 cut(s) 229, 406
Eco57I CTGAAG 1 cut(s) 375
Eco91I GGTNACC 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 59, 327
EcoRV GATATC 2 cut(s) 229, 406
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 542, 553
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 180, 190, 320, 377
FatI CATG 4 cut(s) 176, 186, 316, 373
Fnu4HI GCNGC 5 cut(s) 255, 468, 613, 638, 678
FokI GGATG 2 cut(s) 413, 458
FriOI GRGCYC 2 cut(s) 542, 553
Fsp4HI GCNGC 5 cut(s) 255, 468, 613, 638, 678
FspBI CTAG 3 cut(s) 117, 212, 293
GluI GCNGC 5 cut(s) 255, 468, 613, 638, 678
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 59, 615
Hin1II CATG 4 cut(s) 180, 190, 320, 377
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 9 cut(s) 8, 13, 29, 143, 203, 236, 244, 342, 628
HphI GGTGA 3 cut(s) 144, 429, 514
Hpy166II GTNNAC 2 cut(s) 123, 324
Hpy188I TCNGA 2 cut(s) 288, 387
Hpy188III TCNNGA 4 cut(s) 17, 80, 317, 645
Hpy8I GTNNAC 2 cut(s) 123, 324
HpyAV CCTTC 1 cut(s) 627
HpyCH4V TGCA 5 cut(s) 25, 257, 448, 668, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 269, 298, 674
HpyF3I CTNAG 4 cut(s) 47, 105, 285, 587
Hsp92II CATG 4 cut(s) 180, 190, 320, 377
KspI CCGCGG 1 cut(s) 613
Kzo9I GATC 3 cut(s) 76, 198, 641
LmnI GCTCC 2 cut(s) 43, 379
Lsp1109I GCAGC 3 cut(s) 241, 454, 689
LweI GCATC 2 cut(s) 266, 431
MaeI CTAG 3 cut(s) 117, 212, 293
MaeIII GTNAC 4 cut(s) 150, 280, 435, 590
MalI GATC 3 cut(s) 78, 200, 643
MboI GATC 3 cut(s) 76, 198, 641
MboII GAAGA 3 cut(s) 76, 87, 351
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 542, 553
MluCI AATT 2 cut(s) 332, 358
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 8 cut(s) 34, 42, 45, 49, 64, 125, 360, 493
MslI CAYNNNNRTG 3 cut(s) 191, 480, 495
MspA1I CMGCKG 1 cut(s) 612
MspR9I CCNGG 2 cut(s) 61, 329
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 2 cut(s) 61, 329
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 4 cut(s) 260, 269, 298, 674
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 76, 198, 641
NheI GCTAGC 1 cut(s) 292
NlaIII CATG 4 cut(s) 180, 190, 320, 377
NmuCI GTSAC 3 cut(s) 150, 435, 590
NspI RCATGY 1 cut(s) 377
NspV TTCGAA 2 cut(s) 11, 631
OliI CACNNNNGTG 1 cut(s) 480
PagI TCATGA 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 637
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 8 cut(s) 8, 13, 29, 143, 203, 236, 342, 628
PkrI GCNGC 5 cut(s) 256, 469, 614, 639, 679
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PshAI GACNNNNGTC 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 59, 327
PspEI GGTNACC 1 cut(s) 150
PspGI CCWGG 2 cut(s) 59, 327
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 416, 685
RsaNI GTAC 2 cut(s) 415, 684
RseI CAYNNNNRTG 3 cut(s) 191, 480, 495
SacII CCGCGG 1 cut(s) 613
SatI GCNGC 5 cut(s) 255, 468, 613, 638, 678
Sau3AI GATC 3 cut(s) 76, 198, 641
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 61, 329
SduI GDGCHC 2 cut(s) 542, 553
SfaNI GCATC 2 cut(s) 266, 431
SfcI CTRYAG 1 cut(s) 666
Sfr303I CCGCGG 1 cut(s) 613
SfuI TTCGAA 2 cut(s) 11, 631
SgrBI CCGCGG 1 cut(s) 613
SmiMI CAYNNNNRTG 3 cut(s) 191, 480, 495
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 2 cut(s) 332, 358
SsiI CCGC 4 cut(s) 377, 610, 612, 637
SspMI CTAG 3 cut(s) 117, 212, 293
StyD4I CCNGG 2 cut(s) 59, 327
StyI CCWWGG 1 cut(s) 186
TaqI TCGA 6 cut(s) 11, 167, 201, 247, 626, 631
TasI AATT 2 cut(s) 332, 358
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 615, 640
TfiI GAWTC 8 cut(s) 8, 13, 29, 143, 203, 236, 342, 628
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 3 cut(s) 150, 435, 590
TseI GCWGC 3 cut(s) 254, 467, 677
Tsp45I GTSAC 3 cut(s) 150, 435, 590
TspDTI ATGAA 2 cut(s) 305, 351
TspGWI ACGGA 2 cut(s) 20, 492
TspRI CASTG 1 cut(s) 415
XapI RAATTY 2 cut(s) 332, 358
XceI RCATGY 1 cut(s) 377
XspI CTAG 3 cut(s) 117, 212, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.