Rh1BG187000

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0011092
Physical Location & Seq
Reverse (-)
30192672 .. 30197953
5282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG187000.1

Sequence Viewer

Length: 723 bp
ATGACGGATTCGAATCTGGAGCTCCAGGAATCGGGGTGGGAGGAGCTGAGGAGAGAGGCCCGCAAGATCGAGGGAGATCTTGACGTCAAGCTTTCTTCTTATGCTAAACTCGGAGCTCGGTTCACACAAGGAGGAGGTTATGTGGAATCTGGGTCACCACCTGTTGGGTCCAGCAGGTCATGGAAGTCTATGGAAATGGAAATCCAGTCTTTGCTCGAGAAGCTATTGGATATAAATGATTCAATGAGCCGATGTGCTGCTTCTGCTACACCAGCTACGTCTGTTACTCAGAAGCTAGCAAGGCATAGAGACATACTTCATGAGTTTACTCAGGAGTTCAGACGAATCAAAGGGAACATAAATTCATTGAGGGAACATGCGGAGCTTCTAACTTCTGTCAGGGATGACATTAGCGAGTACAAGGCATCTGGGAGTATGTCTCCAAGAATGCAAATACTACGGGAGAGAGCTGCTATCCATGGAAGTGTATCTCATATAGATGAGGTGATCAGTCAGGCTCAAACAACAAGGGCAGTCTTGGGCTCTCAAAGGGCGTTGTTTGGAGATGTTCAAGGCAAAGTGAAGAATCTAAGTGACAAGTTCCCTGTTATCCGTGGCCTACTTGGTTCAATTAAAAGGAGGCGATCCAGAGACACAATTATTCTGTCTGCAGTCATTGCAGCTTGTACCTTGTTTCTTATCATCTACTGGCTTTCAAAATAG

Protein Analysis

240

Amino Acids

26.78

Weight (kDa)

9.41

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 151 - 215 1.6e-16 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45200 AT2G45200
fragaria_vesca FvH4_7g11270
malus_domestica MD02G1205800.v1.1 MD07G1117300.v1.1
prunus_persica Prupe.2G140500_v2.0.a1 Prupe.2G140500_v2.0.a1
pyrus_communis pycom02g17040 pycom07g10740
rosa_chinensis RchiOBHm_Chr1g0349411
rosa_laevigata RLG00000028551
rosa_multiflora Rmu_sc0002495.1_g000004
rosa_roxburghii Rroxscaffold_4G00305560
rosa_rugosa Rorug01G0203500 Rorug01G0203600.1
rosa_samantha Rh1AG220900 Rh1BG187000 Rh1CG205900 Rh1DG216300
rosa_wichuraiana Rw1G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 165
AccB7I CCANNNNNTGG 1 cut(s) 164
AciI CCGC 2 cut(s) 61, 380
AclWI GGATC 1 cut(s) 639
AcsI RAATTY 1 cut(s) 361
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 2 cut(s) 419, 688
AfiI CCNNNNNNNGG 2 cut(s) 31, 164
AgsI TTSAA 4 cut(s) 243, 572, 630, 717
AjnI CCWGG 1 cut(s) 24
Alw21I GWGCWC 2 cut(s) 24, 118
Alw26I GTCTC 3 cut(s) 303, 444, 645
AlwI GGATC 1 cut(s) 639
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 2 cut(s) 57, 616
ApeKI GCWGC 3 cut(s) 257, 470, 680
ApoI RAATTY 1 cut(s) 361
AspS9I GGNCC 2 cut(s) 58, 168
AsuHPI GGTGA 2 cut(s) 147, 517
AsuII TTCGAA 1 cut(s) 11
AsuNHI GCTAGC 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 3 cut(s) 24, 118, 545
Bbv12I GWGCWC 2 cut(s) 24, 118
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 244, 457, 692
BciT130I CCWGG 1 cut(s) 26
BclI TGATCA 1 cut(s) 507
BcoDI GTCTC 3 cut(s) 303, 444, 645
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 165
BglII AGATCT 1 cut(s) 76
BisI GCNGC 3 cut(s) 258, 471, 681
BlsI GCNGC 3 cut(s) 259, 472, 682
Bme1390I CCNGG 1 cut(s) 26
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 58, 168
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 434
BmtI GCTAGC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 2 cut(s) 8, 38
Bpu10I CCTNAGC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 478, 613
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 164
Bse1I ACTGG 2 cut(s) 205, 713
Bse3DI GCAATG 1 cut(s) 675
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 478, 613
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 31, 164
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 3 cut(s) 38, 302, 344
BseNI ACTGG 2 cut(s) 205, 713
BseRI GAGGAG 3 cut(s) 56, 64, 147
BseXI GCAGC 3 cut(s) 244, 457, 692
BshFI GGCC 2 cut(s) 59, 618
BsiHKAI GWGCWC 2 cut(s) 24, 118
BsiHKCI CYCGRG 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 31, 164
BsmAI GTCTC 3 cut(s) 303, 444, 645
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 2 cut(s) 59, 618
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 24, 118, 545
Bsp143I GATC 4 cut(s) 66, 76, 507, 644
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 2 cut(s) 61, 380
BspANI GGCC 2 cut(s) 59, 618
BspCNI CTCAG 3 cut(s) 39, 301, 343
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 639
BspT104I TTCGAA 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 675
BsrI ACTGG 2 cut(s) 205, 713
BssECI CCNNGG 2 cut(s) 478, 613
BssMI GATC 4 cut(s) 66, 76, 507, 644
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 478
Bst2UI CCWGG 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 61, 297
BstDEI CTNAG 4 cut(s) 47, 288, 330, 590
BstDSI CCRYGG 2 cut(s) 478, 613
BstEII GGTNACC 1 cut(s) 153
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 4 cut(s) 69, 79, 510, 647
BstMAI GTCTC 3 cut(s) 303, 444, 645
BstMBI GATC 4 cut(s) 66, 76, 507, 644
BstMWI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 380
BstPI GGTNACC 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 1 cut(s) 669
BstV1I GCAGC 3 cut(s) 244, 457, 692
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 59, 618
BtgI CCRYGG 2 cut(s) 478, 613
BtsCI GGATG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 61, 297
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 58, 168
Csp6I GTAC 2 cut(s) 418, 687
CviAII CATG 4 cut(s) 180, 320, 377, 479
CviQI GTAC 2 cut(s) 418, 687
DdeI CTNAG 4 cut(s) 47, 288, 330, 590
DpnI GATC 4 cut(s) 68, 78, 509, 646
DpnII GATC 4 cut(s) 66, 76, 507, 644
Ecl136II GAGCTC 2 cut(s) 22, 116
Eco130I CCWWGG 1 cut(s) 478
Eco24I GRGCYC 3 cut(s) 24, 118, 545
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 2 cut(s) 22, 116
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 22, 116
EcoO65I GGTNACC 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 478
EcoT38I GRGCYC 3 cut(s) 24, 118, 545
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 4 cut(s) 183, 323, 380, 482
FatI CATG 4 cut(s) 179, 319, 376, 478
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 258, 471, 681
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 3 cut(s) 24, 118, 545
Fsp4HI GCNGC 3 cut(s) 258, 471, 681
FspBI CTAG 1 cut(s) 296
GluI GCNGC 3 cut(s) 258, 471, 681
GsuI CTGGAG 2 cut(s) 8, 38
HaeIII GGCC 2 cut(s) 59, 618
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 4 cut(s) 183, 323, 380, 482
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
HphI GGTGA 2 cut(s) 147, 517
Hpy166II GTNNAC 2 cut(s) 123, 327
Hpy188I TCNGA 3 cut(s) 113, 291, 341
Hpy188III TCNNGA 6 cut(s) 17, 80, 217, 320, 332, 648
Hpy8I GTNNAC 2 cut(s) 123, 327
HpyCH4IV ACGT 2 cut(s) 84, 278
HpyCH4V TGCA 3 cut(s) 451, 671, 680
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
HpyF3I CTNAG 4 cut(s) 47, 288, 330, 590
HpySE526I ACGT 2 cut(s) 84, 278
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 4 cut(s) 183, 323, 380, 482
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 4 cut(s) 66, 76, 507, 644
LmnI GCTCC 5 cut(s) 19, 27, 43, 113, 382
Lsp1109I GCAGC 3 cut(s) 244, 457, 692
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 84, 278
MaeIII GTNAC 3 cut(s) 153, 283, 593
MalI GATC 4 cut(s) 68, 78, 509, 646
MboI GATC 4 cut(s) 66, 76, 507, 644
MboII GAAGA 2 cut(s) 87, 595
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 3 cut(s) 24, 118, 545
MluCI AATT 3 cut(s) 361, 630, 657
MnlI CCTC 9 cut(s) 34, 42, 49, 64, 125, 128, 363, 496, 633
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 2 cut(s) 483, 498
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
NcoI CCATGG 1 cut(s) 478
NdeII GATC 4 cut(s) 66, 76, 507, 644
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 4 cut(s) 183, 323, 380, 482
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 153, 593
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 319
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
PflMI CCANNNNNTGG 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 24
PkrI GCNGC 3 cut(s) 259, 472, 682
Psp124BI GAGCTC 2 cut(s) 24, 118
Psp6I CCWGG 1 cut(s) 24
PspEI GGTNACC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 2 cut(s) 58, 168
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 419, 688
RsaNI GTAC 2 cut(s) 418, 687
RseI CAYNNNNRTG 2 cut(s) 483, 498
SacI GAGCTC 2 cut(s) 24, 118
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 3 cut(s) 258, 471, 681
Sau3AI GATC 4 cut(s) 66, 76, 507, 644
Sau96I GGNCC 2 cut(s) 58, 168
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 3 cut(s) 24, 118, 545
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 483, 498
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 3 cut(s) 361, 630, 657
SsiI CCGC 2 cut(s) 61, 380
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 24, 118
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 478
TaiI ACGT 2 cut(s) 87, 281
TaqI TCGA 3 cut(s) 11, 69, 216
TasI AATT 3 cut(s) 361, 630, 657
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TseFI GTSAC 2 cut(s) 153, 593
TseI GCWGC 3 cut(s) 257, 470, 680
Tsp45I GTSAC 2 cut(s) 153, 593
TspDTI ATGAA 2 cut(s) 308, 354
TspGWI ACGGA 2 cut(s) 20, 602
Van91I CCANNNNNTGG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 215
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.