AT2G46060

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003810
Physical Location & Seq
Reverse (-)
18941099 .. 18945112
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46060.3

Sequence Viewer

Length: 2424 bp
ATGGCAGAGATTTTGTTTCTGCTTTTAAGAACTCTCGTCGTGTTTTCATGTGCTCTTTCGATCTGTAATTGTCTTCAAGATGCTGCGGACAATAATGGAAACTTTTTCACGGTCTCGAGCTTCAGGTACCCTGAATCCGAAGTCAGACCCTACGATACACGTTACATCAGAGTTGATTTGCCTCCATGGTTCTCATCATTGAATGTTGCTATTGAATCTGATGTAGACATCACTGCAAAGAGCATTTCGAAGATTTCCAAAAGTCTTCTTCCTGTTATATGCTTTCGAGATGGTAGTCCTCCTCTGCCTGATGCCTCAACCAACGCTCTTCAAGGATTAGAGCTAGGACGGTTCTTTAATGGATCTTTTGAAAGAGCTCAAGATAGTGAGATTGCACAACAGTGTTACCCTATGCAGAAGAATATAACCTTGAGATTGACCAATGAACAGATATCTCCTGGAGCTTGGTATGTTGGTCTCTTTAACGGAATTGGGGCGACTAGGACTCAGGGGAAAATGATTGTTCGCTCCTCTGCGTTTTCATTTAGTGCCAACATTAGTGTTGAAGGTTGTAAAACCGCTACAATGTGGGGGCCTTTCTGCAACCAGACTATTTATCCGCTTTCATGTTCTCGGTTCGATAACCAGACAGCAAGTGTTATTTCTTGCGCTGATTCTTTTCCTAGTTCTTGTCTGACTGGTGCTGAAACGAAGACATATGCTTTAGATGTAGATGGAATAGCTGAACAGTTAGTTATTATGGCATCCAATGTCAAAGTAGATTCCAATGAAAGTTATCTCATGTGTTACGCCCGTTTTGAAGCCTTTGCTTCAGAGACTCTGCATGATTACGCTGCTGATATACATAAAGTTCCCTTAATTGTTAACAAACCGAAAGCTGGTCGATGGTATATAGTTATTAGTTTATCTGGACGGGAGAACCGGTTTGCACAGGGCACAAATTCCAGTTCTAGGGTCTGCTTTTCCATTAATGTTAAAGTACTCGGATGTCCTGTAGGAAAAGCAGGACCAAATTGTGGGCAGCAAATCTACATTCTTCAGGCAGTTATGAGGAGAGGATGGTTGACACCTTTTCAATCATATTATTTTCCGGTCAATGACGCTTCACTTTCAGGCTCGAGCACAAATTTCCCCCTCGAACCCATCGTCAGCAACTTTTCCTCTATTCCAGAATTAGACACAAGCACATGGACTTATTTTCTCATGAACATCCCTCAAGGTGGTTCTGGTGGACATATCCATTTCAGATTATTATCAGATTCCACAATACAGTATGAAGTATATCTTAGATTTGGTGGATTGCCCACTATTGACGACCGGGATTACTATTATGTAAATCGAACAAGTGCCAGCCGCTCAATGTTCTTCTCATTGTATAATTCTAGCAAAGAAATGGTTGATTTCTACATCTTGTATGCTCGAGAAGGAACCTGGTCATTTGGGTTAAGGCAGCTCATTGACTCTAATACTCCTGCTGCTTCCAGAGGTTCACCTACTTTAGTATCTCTTTCTCTCGAAAGATGCCCGAGAGGGTGTTCGTCTTATGGGCAATGCCGATATGCTTTTGACGCTAATGGATTGACATCTTACAGCTTTTGTTCTTGTGACAGAACCCACGGAGGCTTTGACTGCAGCATTGAAATTGTGTCACACCAAGAACATATTGTTCAGTCTATTGCTCTCATCGCATCAAATGCAGCGGCTCTTTTGCCAGCTTACTGGGCTCTTCGGCAGCGGGAGTATCCAGAGTGGGTTCTGTTTACATCCAGCGGAATCTCGAGCGCTCTGTATCATGCATGTGATGTAGGCACCTGGTGTGTTTTAAGTTACAATGTTTTACAGTTCATGGATTTCTGGCTCTCTTTCATGGCCGTGGTTGGTACATTTGTGTACTTATCCACGGCTGGTGAAGCAGTTAAAAGGACAATACACACAGTTGTTGCCATTCTCACAGCTTTATTGGCCTTGACTCAAGCAACAAGGGCGTCAAATATTATCATTGTGTTAGCAATCGGTTCACTCGGTCTCCTCATCGGATTTTTGGTAGAATTTGTTACCAAGTATCGATCATACTGCGGGTCGGCTGGATTTTCATTGAACATGCTCGACAGGCCACGGGCAGTCAAAGAATGGTTTAGTAATTTGATCAAGACGCTCAAGAAACGGTTTCGTTGGGGCTTTGTGGCGGCTGGTCTTGTAGCCTTCACCATGGCAGCCATAAGTTTCAAAGTAGAAACCAGTTCGAGCTACTGGATGTGGCACAGTATTTGGCATTTCACAATCTACACATCTTCTTTCTTCTTCCTCTGTTCGAAGATTGCAATTGTAAATCACGAGAACCTAGCCCACAACGGAGCTGACAACTACGAGTTAACAAGGCAAGACTCGCTATCAAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

807

Amino Acids

89.79

Weight (kDa)

7.42

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 554 - 760 2e-43 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 126, 1829
AccB7I CCANNNNNTGG 1 cut(s) 1037
AccBSI CCGCTC 1 cut(s) 1377
AccI GTMKAC 1 cut(s) 225
AciI CCGC 9 cut(s) 86, 579, 620, 1375, 1721, 1756, 1791, 2097, 2207
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 1890
AcsI RAATTY 3 cut(s) 961, 1147, 2069
AcuI CTGAAG 3 cut(s) 106, 816, 1043
AcyI GRCGYC 1 cut(s) 2006
AdeI CACNNNGTG 1 cut(s) 1836
AfaI GTAC 4 cut(s) 128, 1002, 1903, 1913
AfeI AGCGCT 1 cut(s) 1804
AfiI CCNNNNNNNGG 4 cut(s) 899, 972, 1037, 1241
AflIII ACRYGT 1 cut(s) 158
AgeI ACCGGT 1 cut(s) 942
AjnI CCWGG 3 cut(s) 457, 1451, 1832
AleI CACNNNNGTG 1 cut(s) 400
Alw21I GWGCWC 3 cut(s) 55, 379, 1145
Alw26I GTCTC 4 cut(s) 118, 482, 830, 2051
AlwI GGATC 1 cut(s) 370
Ama87I CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
Aor51HI AGCGCT 1 cut(s) 1804
AoxI GGCC 4 cut(s) 593, 1890, 1983, 2132
ApeKI GCWGC 9 cut(s) 83, 854, 1042, 1471, 1496, 1653, 1718, 1753, 2234
ApoI RAATTY 3 cut(s) 961, 1147, 2069
ArsI GACNNNNNNTTYG 2 cut(s) 1152, 1184
AseI ATTAAT 1 cut(s) 990
AsiGI ACCGGT 1 cut(s) 942
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 2 cut(s) 671, 1805
AspS9I GGNCC 2 cut(s) 593, 1028
AsuC2I CCSGG 1 cut(s) 1340
AsuHPI GGTGA 3 cut(s) 1503, 1940, 2218
AsuII TTCGAA 2 cut(s) 248, 2333
AvaI CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
AvaII GGWCC 1 cut(s) 1028
BaeGI GKGCMC 1 cut(s) 959
BaeI ACNNNNGTAYC 4 cut(s) 1506, 1539, 1893, 1926
BanI GGYRCC 2 cut(s) 126, 1829
BanII GRGCYC 2 cut(s) 379, 1747
BauI CACGAG 1 cut(s) 2354
BbsI GAAGAC 3 cut(s) 65, 257, 719
Bbv12I GWGCWC 3 cut(s) 55, 379, 1145
BbvI GCAGC 9 cut(s) 70, 841, 1054, 1483, 1483, 1665, 1730, 1765, 2246
BccI CCATC 5 cut(s) 284, 728, 900, 1074, 1172
BceAI ACGGC 2 cut(s) 1877, 1938
BcgI CGANNNNNNTGC 2 cut(s) 2076, 2110
BciT130I CCWGG 3 cut(s) 459, 1453, 1834
BciVI GTATCC 1 cut(s) 1773
BclI TGATCA 1 cut(s) 2166
BcnI CCSGG 1 cut(s) 1340
BcoDI GTCTC 4 cut(s) 118, 482, 830, 2051
BfaI CTAG 6 cut(s) 344, 501, 684, 972, 1404, 2363
BfmI CTRYAG 2 cut(s) 1014, 1651
BfoI RGCGCY 1 cut(s) 1806
BfuI GTATCC 1 cut(s) 1773
BmcAI AGTACT 1 cut(s) 1002
Bme1390I CCNGG 4 cut(s) 459, 1340, 1453, 1834
Bme18I GGWCC 1 cut(s) 1028
BmeT110I CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
BmgT120I GGNCC 2 cut(s) 593, 1028
BmiI GGNNCC 4 cut(s) 128, 594, 1450, 1831
BmrFI CCNGG 4 cut(s) 459, 1340, 1453, 1834
BmrI ACTGGG 1 cut(s) 1750
BmsI GCATC 5 cut(s) 70, 301, 773, 1532, 1718
BmuI ACTGGG 1 cut(s) 1750
BpiI GAAGAC 3 cut(s) 65, 257, 719
BpmI CTGGAG 1 cut(s) 480
Bpu14I TTCGAA 2 cut(s) 248, 2333
BpuEI CTTGAG 5 cut(s) 363, 451, 1221, 1977, 2162
BpuMI CCSGG 1 cut(s) 1340
Bsa29I ATCGAT 1 cut(s) 2086
BsaBI GATNNNNATC 2 cut(s) 1273, 1603
BsaHI GRCGYC 1 cut(s) 2006
BsaI GGTCTC 3 cut(s) 118, 482, 2051
BsaJI CCNNGG 6 cut(s) 185, 1636, 1893, 1920, 2135, 2229
BsaWI WCCGGW 2 cut(s) 942, 1111
BsaXI ACNNNNNCTCC 4 cut(s) 929, 959, 2031, 2061
Bsc4I CCNNNNNNNGG 4 cut(s) 899, 972, 1037, 1241
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 5 cut(s) 703, 966, 1745, 2259, 2276
Bse3DI GCAATG 1 cut(s) 1577
Bse8I GATNNNNATC 2 cut(s) 1273, 1603
BseBI CCWGG 3 cut(s) 459, 1453, 1834
BseCI ATCGAT 1 cut(s) 2086
BseDI CCNNGG 6 cut(s) 185, 1636, 1893, 1920, 2135, 2229
BseGI GGATG 6 cut(s) 764, 1013, 1085, 1230, 1784, 2280
BseJI GATNNNNATC 2 cut(s) 1273, 1603
BseLI CCNNNNNNNGG 4 cut(s) 899, 972, 1037, 1241
BseMI GCAATG 1 cut(s) 1577
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 5 cut(s) 703, 966, 1745, 2259, 2276
BseRI GAGGAG 4 cut(s) 291, 520, 1087, 2039
BseSI GKGCMC 1 cut(s) 959
BseXI GCAGC 9 cut(s) 70, 841, 1054, 1483, 1483, 1665, 1730, 1765, 2246
Bsh1285I CGRYCG 1 cut(s) 1339
BshFI GGCC 4 cut(s) 595, 1892, 1985, 2134
BshNI GGYRCC 2 cut(s) 126, 1829
BshTI ACCGGT 1 cut(s) 942
BshVI ATCGAT 1 cut(s) 2086
BsiEI CGRYCG 1 cut(s) 1339
BsiHKAI GWGCWC 3 cut(s) 55, 379, 1145
BsiHKCI CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
BsiSI CCGG 3 cut(s) 943, 1112, 1339
BslI CCNNNNNNNGG 4 cut(s) 899, 972, 1037, 1241
BsmAI GTCTC 4 cut(s) 118, 482, 830, 2051
BsnI GGCC 4 cut(s) 595, 1892, 1985, 2134
Bso31I GGTCTC 3 cut(s) 118, 482, 2051
BsoBI CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
Bsp119I TTCGAA 2 cut(s) 248, 2333
Bsp1286I GDGCHC 5 cut(s) 55, 379, 959, 1145, 1747
Bsp143I GATC 4 cut(s) 60, 362, 2087, 2166
Bsp19I CCATGG 2 cut(s) 185, 2229
BspACI CCGC 9 cut(s) 86, 579, 620, 1375, 1721, 1756, 1791, 2097, 2207
BspANI GGCC 4 cut(s) 595, 1892, 1985, 2134
BspCNI CTCAG 1 cut(s) 520
BspDI ATCGAT 1 cut(s) 2086
BspHI TCATGA 1 cut(s) 1224
BspLI GGNNCC 4 cut(s) 128, 594, 1450, 1831
BspMAI CTGCAG 1 cut(s) 1655
BspPI GGATC 1 cut(s) 370
BspQI GCTCTTC 2 cut(s) 333, 1752
BspT104I TTCGAA 2 cut(s) 248, 2333
BspT107I GGYRCC 2 cut(s) 126, 1829
BspTNI GGTCTC 3 cut(s) 118, 482, 2051
BsrBI CCGCTC 1 cut(s) 1377
BsrDI GCAATG 1 cut(s) 1577
BsrFI RCCGGY 1 cut(s) 942
BsrI ACTGG 5 cut(s) 703, 966, 1745, 2259, 2276
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 6 cut(s) 185, 1636, 1893, 1920, 2135, 2229
BssMI GATC 4 cut(s) 60, 362, 2087, 2166
BssNI GRCGYC 1 cut(s) 2006
BssSI CACGAG 1 cut(s) 2354
BssT1I CCWWGG 2 cut(s) 185, 2229
Bst2BI CACGAG 1 cut(s) 2354
Bst2UI CCWGG 3 cut(s) 459, 1453, 1834
Bst4CI ACNGT 9 cut(s) 112, 351, 402, 750, 1293, 1863, 1957, 2187, 2285
Bst6I CTCTTC 2 cut(s) 333, 1752
BstACI GRCGYC 1 cut(s) 2006
BstAPI GCANNNNNTGC 1 cut(s) 1715
BstBI TTCGAA 2 cut(s) 248, 2333
BstC8I GCNNGC 2 cut(s) 1372, 1734
BstDEI CTNAG 2 cut(s) 507, 1307
BstDSI CCRYGG 6 cut(s) 185, 1636, 1893, 1920, 2135, 2229
BstF5I GGATG 6 cut(s) 764, 1013, 1085, 1230, 1784, 2280
BstH2I RGCGCY 1 cut(s) 1806
BstHHI GCGC 2 cut(s) 671, 1805
BstKTI GATC 4 cut(s) 63, 365, 2090, 2169
BstMAI GTCTC 4 cut(s) 118, 482, 830, 2051
BstMBI GATC 4 cut(s) 60, 362, 2087, 2166
BstMCI CGRYCG 1 cut(s) 1339
BstNI CCWGG 3 cut(s) 459, 1453, 1834
BstNSI RCATGY 2 cut(s) 1821, 2125
BstSCI CCNGG 4 cut(s) 457, 1338, 1451, 1832
BstSFI CTRYAG 2 cut(s) 1014, 1651
BstSLI GKGCMC 1 cut(s) 959
BstV1I GCAGC 9 cut(s) 70, 841, 1054, 1483, 1483, 1665, 1730, 1765, 2246
BstV2I GAAGAC 3 cut(s) 65, 257, 719
BstX2I RGATCY 1 cut(s) 362
BstXI CCANNNNNNTGG 1 cut(s) 1740
BstYI RGATCY 1 cut(s) 362
Bsu15I ATCGAT 1 cut(s) 2086
BsuI GTATCC 1 cut(s) 1773
BsuRI GGCC 4 cut(s) 595, 1892, 1985, 2134
BsuTUI ATCGAT 1 cut(s) 2086
BtgI CCRYGG 6 cut(s) 185, 1636, 1893, 1920, 2135, 2229
BtgZI GCGATG 1 cut(s) 1690
BtsCI GGATG 6 cut(s) 764, 1013, 1085, 1230, 1784, 2280
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 231, 407
Cac8I GCNNGC 2 cut(s) 1372, 1734
CciI TCATGA 1 cut(s) 1224
CfoI GCGC 2 cut(s) 671, 1805
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 2 cut(s) 593, 1028
ClaI ATCGAT 1 cut(s) 2086
CseI GACGC 4 cut(s) 1130, 1598, 1995, 2182
CsiI ACCWGGT 2 cut(s) 1451, 1832
Csp6I GTAC 4 cut(s) 127, 1001, 1902, 1912
CspAI ACCGGT 1 cut(s) 942
CviQI GTAC 4 cut(s) 127, 1001, 1902, 1912
DdeI CTNAG 2 cut(s) 507, 1307
DpnI GATC 4 cut(s) 62, 364, 2089, 2168
DpnII GATC 4 cut(s) 60, 362, 2087, 2166
DraIII CACNNNGTG 1 cut(s) 1836
EaeI YGGCCR 1 cut(s) 1890
Eam1104I CTCTTC 2 cut(s) 333, 1752
EarI CTCTTC 2 cut(s) 333, 1752
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 185, 2229
Eco24I GRGCYC 2 cut(s) 379, 1747
Eco31I GGTCTC 3 cut(s) 118, 482, 2051
Eco32I GATATC 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 1028
Eco47III AGCGCT 1 cut(s) 1804
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 3 cut(s) 106, 816, 1043
Eco88I CYCGRG 5 cut(s) 115, 1138, 1440, 1546, 1798
EcoICRI GAGCTC 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 457, 1451, 1832
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 2 cut(s) 185, 2229
EcoT22I ATGCAT 1 cut(s) 1819
EcoT38I GRGCYC 2 cut(s) 379, 1747
ErhI CCWWGG 2 cut(s) 185, 2229
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FauI CCCGC 2 cut(s) 1749, 2090
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 1 cut(s) 2166
FblI GTMKAC 1 cut(s) 225
FokI GGATG 6 cut(s) 751, 1020, 1092, 1217, 1771, 2287
FriOI GRGCYC 2 cut(s) 379, 1747
FspBI CTAG 6 cut(s) 344, 501, 684, 972, 1404, 2363
GlaI GCGC 2 cut(s) 670, 1804
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 1806
HaeIII GGCC 4 cut(s) 595, 1892, 1985, 2134
HapII CCGG 3 cut(s) 943, 1112, 1339
HgaI GACGC 4 cut(s) 1130, 1598, 1995, 2182
HhaI GCGC 2 cut(s) 671, 1805
Hin1I GRCGYC 1 cut(s) 2006
Hin6I GCGC 2 cut(s) 669, 1803
HinP1I GCGC 2 cut(s) 669, 1803
HincII GTYRAC 3 cut(s) 886, 1086, 2394
HindII GTYRAC 3 cut(s) 886, 1086, 2394
HpaI GTTAAC 2 cut(s) 886, 2394
HpaII CCGG 3 cut(s) 943, 1112, 1339
HphI GGTGA 3 cut(s) 1503, 1940, 2218
Hpy166II GTNNAC 9 cut(s) 226, 886, 1086, 1253, 1511, 1782, 1912, 2039, 2394
Hpy8I GTNNAC 9 cut(s) 226, 886, 1086, 1253, 1511, 1782, 1912, 2039, 2394
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 560, 1439, 2233
HpyCH4III ACNGT 9 cut(s) 112, 351, 402, 750, 1293, 1863, 1957, 2187, 2285
HpyCH4IV ACGT 1 cut(s) 160
HpyF3I CTNAG 2 cut(s) 507, 1307
HpySE526I ACGT 1 cut(s) 160
Hsp92I GRCGYC 1 cut(s) 2006
HspAI GCGC 2 cut(s) 669, 1803
KpnI GGTACC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 2166
KspAI GTTAAC 2 cut(s) 886, 2394
Kzo9I GATC 4 cut(s) 60, 362, 2087, 2166
LguI GCTCTTC 2 cut(s) 333, 1752
LmnI GCTCC 3 cut(s) 461, 533, 2375
Lsp1109I GCAGC 9 cut(s) 70, 841, 1054, 1483, 1483, 1665, 1730, 1765, 2246
LweI GCATC 5 cut(s) 70, 301, 773, 1532, 1718
MabI ACCWGGT 2 cut(s) 1451, 1832
MaeI CTAG 6 cut(s) 344, 501, 684, 972, 1404, 2363
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 7 cut(s) 161, 404, 806, 1625, 1668, 1847, 2074
MalI GATC 4 cut(s) 62, 364, 2089, 2168
MbiI CCGCTC 1 cut(s) 1377
MboI GATC 4 cut(s) 60, 362, 2087, 2166
MfeI CAATTG 1 cut(s) 2343
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 5 cut(s) 55, 379, 959, 1145, 1747
MlyI GAGTC 5 cut(s) 499, 832, 1475, 1984, 2399
Mph1103I ATGCAT 1 cut(s) 1819
MslI CAYNNNNRTG 3 cut(s) 400, 1818, 1892
MspA1I CMGCKG 3 cut(s) 1721, 1756, 1791
MspI CCGG 3 cut(s) 943, 1112, 1339
MspR9I CCNGG 4 cut(s) 459, 1340, 1453, 1834
MunI CAATTG 1 cut(s) 2343
MvaI CCWGG 3 cut(s) 459, 1453, 1834
NciI CCSGG 1 cut(s) 1340
NcoI CCATGG 2 cut(s) 185, 2229
NdeI CATATG 1 cut(s) 718
NdeII GATC 4 cut(s) 60, 362, 2087, 2166
NlaIV GGNNCC 4 cut(s) 128, 594, 1450, 1831
NmuCI GTSAC 2 cut(s) 1625, 1668
NsiI ATGCAT 1 cut(s) 1819
NspI RCATGY 2 cut(s) 1821, 2125
NspV TTCGAA 2 cut(s) 248, 2333
OliI CACNNNNGTG 1 cut(s) 400
PaeR7I CTCGAG 4 cut(s) 115, 1138, 1440, 1798
PagI TCATGA 1 cut(s) 1224
PciSI GCTCTTC 2 cut(s) 333, 1752
PcsI WCGNNNNNNNCGW 1 cut(s) 1164
PfeI GAWTC 6 cut(s) 134, 215, 674, 782, 1280, 1794
PflMI CCANNNNNTGG 1 cut(s) 1037
PfoI TCCNGGA 1 cut(s) 457
PinAI ACCGGT 1 cut(s) 942
PleI GAGTC 5 cut(s) 499, 832, 1475, 1984, 2399
PpsI GAGTC 5 cut(s) 499, 832, 1475, 1984, 2399
PshBI ATTAAT 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 457, 1451, 1832
PspGI CCWGG 3 cut(s) 457, 1451, 1832
PspN4I GGNNCC 4 cut(s) 128, 594, 1450, 1831
PspPI GGNCC 2 cut(s) 593, 1028
PspXI VCTCGAGB 1 cut(s) 1138
PstI CTGCAG 1 cut(s) 1655
PsuI RGATCY 1 cut(s) 362
RsaI GTAC 4 cut(s) 128, 1002, 1903, 1913
RsaNI GTAC 4 cut(s) 127, 1001, 1902, 1912
RseI CAYNNNNRTG 3 cut(s) 400, 1818, 1892
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 2 cut(s) 333, 1752
Sau3AI GATC 4 cut(s) 60, 362, 2087, 2166
Sau96I GGNCC 2 cut(s) 593, 1028
ScaI AGTACT 1 cut(s) 1002
SchI GAGTC 5 cut(s) 499, 832, 1475, 1984, 2399
ScrFI CCNGG 4 cut(s) 459, 1340, 1453, 1834
SduI GDGCHC 5 cut(s) 55, 379, 959, 1145, 1747
SexAI ACCWGGT 2 cut(s) 1451, 1832
SfaNI GCATC 5 cut(s) 70, 301, 773, 1532, 1718
SfcI CTRYAG 2 cut(s) 1014, 1651
Sfr274I CTCGAG 4 cut(s) 115, 1138, 1440, 1798
SfuI TTCGAA 2 cut(s) 248, 2333
SinI GGWCC 1 cut(s) 1028
SlaI CTCGAG 4 cut(s) 115, 1138, 1440, 1798
SmiMI CAYNNNNRTG 3 cut(s) 400, 1818, 1892
SmlI CTYRAG 9 cut(s) 115, 378, 430, 1138, 1236, 1440, 1798, 1992, 2177
SmoI CTYRAG 9 cut(s) 115, 378, 430, 1138, 1236, 1440, 1798, 1992, 2177
SsiI CCGC 9 cut(s) 86, 579, 620, 1375, 1721, 1756, 1791, 2097, 2207
SspI AATATT 1 cut(s) 2014
SspMI CTAG 6 cut(s) 344, 501, 684, 972, 1404, 2363
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 4 cut(s) 457, 1338, 1451, 1832
StyI CCWWGG 2 cut(s) 185, 2229
TaaI ACNGT 9 cut(s) 112, 351, 402, 750, 1293, 1863, 1957, 2187, 2285
TaiI ACGT 1 cut(s) 163
TaqII GACCGA 1 cut(s) 2033
TatI WGTACW 2 cut(s) 1000, 1911
TauI GCSGC 3 cut(s) 1377, 1724, 2210
TfiI GAWTC 6 cut(s) 134, 215, 674, 782, 1280, 1794
TscAI CASTG 2 cut(s) 238, 407
TseFI GTSAC 2 cut(s) 1625, 1668
TseI GCWGC 9 cut(s) 83, 854, 1042, 1471, 1496, 1653, 1718, 1753, 2234
Tsp45I GTSAC 2 cut(s) 1625, 1668
TspGWI ACGGA 3 cut(s) 501, 1653, 2388
TspRI CASTG 2 cut(s) 238, 407
Van91I CCANNNNNTGG 1 cut(s) 1037
VpaK11BI GGWCC 1 cut(s) 1028
VspI ATTAAT 1 cut(s) 990
XapI RAATTY 3 cut(s) 961, 1147, 2069
XceI RCATGY 2 cut(s) 1821, 2125
XhoI CTCGAG 4 cut(s) 115, 1138, 1440, 1798
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 6 cut(s) 344, 501, 684, 972, 1404, 2363
ZrmI AGTACT 1 cut(s) 1002
Zsp2I ATGCAT 1 cut(s) 1819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.