MD07G1133800.v1.1

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003810
Physical Location & Seq
Forward (+)
19542032 .. 19548219
6188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1133800.v1.1.491

Sequence Viewer

Length: 2496 bp
ATGGCTTCGAATTCGATTCTTTGTTCCTCTTCCCATTTAAATCTCAGTCTTTTCGTTCTCTTTTTCTGCTTATTCGTCCACTGCTATTCGTACGGCGAACTGGGTCCTTACACCAGCTTCACTGTCTCCAGCTTCAGCTACCCACCGACCACAATCGGACCCTCTGGATTTCGATACATTAGAGTTGAAATGCCACCATGGTTCTCGTCGATGTCAATAGCAATGATTTCGAATGTGGAACTTGCTGCTGGAGACATAGAAAAGGTTCCAAAAATTAAGTTACCGTTGATTTGCTTCCGAGCTGGTAGCCTCCCACTGCCGGATGTTAACACTTCTCTAGTGGATTCAGTTCTAGCTCCCCTCTACAATGGTTCCTATAAGGGAATACAAGCTCTTCAGAGTTCAGAGCAGTGCTATCCTATGCAAAAGAATTTTACAATCAAGTTGACAAATGAGCAGATTTCTCCAGGAGTTTGGTATTTTGGTCTTTTCAATGGAATTGGACCTACAAGGACACAATCAAAGATGATTAATCGCTCACCATCATACACCTTCAGTGCCAATATCACTGTGGAAGGATGTACGACCCTAACCATGTGGGGCCCAGACTGCAATCAGACAATTAACCCACTTTCTTGTGCTCTATCTAACAGTTACAGTGCTGCAGACAATTCTTCAGAAGCTGGTTTGTATAACCAAACACTTGAATATGTAATTTCATGCAAAAACAATTTTGGCACTTGTCATGGAGATGGCGAGCCCACATTCTATTCTTTGGATGTAGTGGGAGTATCACAAGAGTTGAAAATTTTGGCAAAGGATGTTTGGTTAAATGTAACGTTTTCAAATAACACCAAGAATGTTAGTGATGTTAATTTAATGTGTTATGCACGGCATGGTGCAATACCTTCAGAAACTGTGCATGACTATTCCAGTAATATAAACAAATCCCCGTTGGTCATTCAATTTCCAAAGGTCGGTAGCTGGTATATTACTATTTTACCTCTCAACGTTTCAAAGGAGCTTGGAGGGAGTCTGGATACTGACACAAAAGTTTGTTATTCCCTGCAATCAAAACTGCTTGAATGTCCGGAGGGCAAGGCCGGAGCAAATTGTACAAGGGAAATATACACCCTTCAGACATCACTGAGGAAAGGTTCAAGCTACTTTGAATACTACTATTTACCCGTCAGTGAAAAAGTGTCTCCTGATTCGGCCAATTTTCCTCTTGATCCTCTTTCAACCAACTCCTCGCATGGTGGACAACCAGAAGAAGCATGGACTTACTTTGTCATGGACATTCCGCGTGGAGCAGCTGGAGGAAACATGCACATTCGATTAAACTCAGATGAAAAGGTTAACTATGAAGTATATGCTAGATACGGTGGATTACCATCTCTTGCTAGCTGGGACTATTTTTATTCAAACAGGACAAGCAGTAGTGATAGCTCCATGTTTTTTAACTTATATAATTCAAGTGAAGACAAGGTCGATTTTTACATTTTGTACGTTAGAGAAGGGACTTGGAGCTTTGGTCTAAAGCATCTCAACACCACCAGTGATGCTTTGAAAGTTCCGACTAGCATGTCTGTTTCACTTGAAAGATGCCCGAGAAGGTGCTCTTCACATGGAAAATGCGAAACTGCATTGGATGCCAGTGGATTGACATCATACAGCTTCTGCTCATGTGATCGAAACCATGGAGGCTTTGACTGTAGTATTCAACTTGTAACACCTCATGGGCATGTATGGCAATCCATTTTTCTTATTGCTTCAAATGCTGCAGCAGCACTTCCTGCATTTTGGGCCCTTCGACAGAAGGCATTGGCAGAGTGGGTGATATTTACATCTAGTGGAATTGCAAGCGGAATATATCATGCATGTGATGTGGGAACCTGGTGTCCTCTGGGCTTTGGTGTCTTACAGTTCATGGACTTCTGGCTCTCTTTCATGGCCGTAGTGAGCACATTTGTGTACCTAGGCACATTTGAGGACGATCTTAAGAGAGCAGTACACACAGCTGTTGCAATCCTTACTGCCCTTATGGCTTATACTAAGGCCACAAGGCCGGCCAATATTATTCTTGTAATGGTAATTGGCTCTGCGGCTCTTCTTATTGGGTGGCTGATAGAATTGTCAACCAAGTTTAGGTCATTTTCCTTTTCGACCGGATTCTCCTTAAATATGATTGAGAGGTTGCAAGCTGTTAGGGGATGGCTCAAGAACCTCATTAAGACAATTATTAAAAGATTTCGCTGGGGTTTTGTACTGGCAGGTTTAGTTGCATTAGCCATTGCAGCAAGTAGCTGGGTACTGGAGACCAGTGAAAGCTACTGGATTTGGCACAGCATTTGGCACATAACAATATATACATCTTCCTTCTTCTTCCTATGCTCGAAAGCGAGTCCTGCAACTACGGTAGATACTGAGAATCAAATACCACAGAATGGAGCCTATGAATTGACTCGACAAGATTCATTTTCAAGAGGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

832

Amino Acids

92.05

Weight (kDa)

6.36

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 582 - 787 5.7e-42 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1585
Acc36I ACCTGC 1 cut(s) 2264
AccB7I CCANNNNNTGG 1 cut(s) 2447
AccII CGCG 1 cut(s) 1306
AccIII TCCGGA 1 cut(s) 1090
AciI CCGC 3 cut(s) 1304, 1866, 2105
AclI AACGTT 2 cut(s) 839, 1011
AclWI GGATC 1 cut(s) 1226
AcoI YGGCCR 3 cut(s) 1215, 1953, 2070
AcsI RAATTY 3 cut(s) 10, 430, 807
AcuI CTGAAG 6 cut(s) 118, 380, 538, 660, 894, 1121
AfaI GTAC 8 cut(s) 92, 583, 1117, 1508, 1976, 2013, 2268, 2313
AfiI CCNNNNNNNGG 4 cut(s) 319, 977, 2148, 2447
AflII CTTAAG 1 cut(s) 2000
AjnI CCWGG 2 cut(s) 466, 1895
AleI CACNNNNGTG 1 cut(s) 1970
AloI GAACNNNNNNTCC 2 cut(s) 1885, 1917
Alw21I GWGCWC 3 cut(s) 643, 1622, 1967
Alw26I GTCTC 4 cut(s) 130, 246, 1209, 2312
AlwI GGATC 1 cut(s) 1226
AlwNI CAGNNNCTG 3 cut(s) 683, 917, 1680
Ama87I CYCGRG 1 cut(s) 1609
Aor13HI TCCGGA 1 cut(s) 1090
AoxI GGCC 9 cut(s) 601, 1101, 1215, 1806, 1953, 2058, 2066, 2070, 2488
ApaI GGGCCC 2 cut(s) 605, 1810
ApeKI GCWGC 7 cut(s) 245, 662, 1313, 1781, 1784, 1787, 2297
ApoI RAATTY 3 cut(s) 10, 430, 807
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 264
AspA2I CCTAGG 1 cut(s) 1978
AspS9I GGNCC 7 cut(s) 104, 158, 503, 601, 602, 1806, 1807
AsuHPI GGTGA 2 cut(s) 531, 1849
AsuII TTCGAA 2 cut(s) 8, 230
AsuNHI GCTAGC 1 cut(s) 1403
AvaI CYCGRG 1 cut(s) 1609
AvaII GGWCC 3 cut(s) 104, 158, 503
AvrII CCTAGG 1 cut(s) 1978
BaeGI GKGCMC 2 cut(s) 605, 1810
BanII GRGCYC 3 cut(s) 605, 762, 1810
BbsI GAAGAC 1 cut(s) 1488
Bbv12I GWGCWC 3 cut(s) 643, 1622, 1967
BbvI GCAGC 7 cut(s) 232, 649, 1325, 1768, 1796, 1799, 2309
BccI CCATC 4 cut(s) 550, 746, 1402, 2208
BceAI ACGGC 3 cut(s) 109, 908, 1940
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 2 cut(s) 468, 1897
BciVI GTATCC 1 cut(s) 1033
BcoDI GTCTC 4 cut(s) 130, 246, 1209, 2312
BfaI CTAG 7 cut(s) 338, 353, 1377, 1404, 1581, 1851, 1979
BfmI CTRYAG 3 cut(s) 663, 1714, 1782
BfrI CTTAAG 1 cut(s) 2000
BfuAI ACCTGC 1 cut(s) 2264
BfuI GTATCC 1 cut(s) 1033
BglI GCCNNNNNGGC 1 cut(s) 2045
BisI GCNGC 8 cut(s) 246, 663, 1314, 1782, 1785, 1788, 2106, 2298
BlnI CCTAGG 1 cut(s) 1978
BlsI GCNGC 8 cut(s) 247, 664, 1315, 1783, 1786, 1789, 2107, 2299
Bme1390I CCNGG 2 cut(s) 468, 1897
Bme18I GGWCC 3 cut(s) 104, 158, 503
BmeT110I CYCGRG 1 cut(s) 1609
BmgT120I GGNCC 7 cut(s) 104, 158, 503, 601, 602, 1806, 1807
BmiI GGNNCC 9 cut(s) 105, 160, 267, 373, 602, 603, 1808, 1894, 2452
BmrFI CCNGG 2 cut(s) 468, 1897
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 4 cut(s) 1552, 1552, 1595, 1642
BmtI GCTAGC 1 cut(s) 1407
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 1488
BpmI CTGGAG 5 cut(s) 112, 270, 450, 1338, 2336
Bpu14I TTCGAA 2 cut(s) 8, 230
BpuEI CTTGAG 1 cut(s) 2204
BsaBI GATNNNNATC 2 cut(s) 1393, 1666
BsaI GGTCTC 1 cut(s) 2312
BsaJI CCNNGG 3 cut(s) 197, 1699, 1978
BsaWI WCCGGW 2 cut(s) 1090, 2168
BsaXI ACNNNNNCTCC 2 cut(s) 1519, 1549
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 977, 2148, 2447
Bse118I RCCGGY 1 cut(s) 2068
Bse1I ACTGG 8 cut(s) 105, 933, 1557, 1656, 2274, 2319, 2322, 2339
Bse3DI GCAATG 2 cut(s) 228, 2292
Bse8I GATNNNNATC 2 cut(s) 1393, 1666
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 2 cut(s) 468, 1897
BseDI CCNNGG 3 cut(s) 197, 1699, 1978
BseGI GGATG 6 cut(s) 328, 584, 784, 826, 1657, 2219
BseJI GATNNNNATC 2 cut(s) 1393, 1666
BseLI CCNNNNNNNGG 4 cut(s) 319, 977, 2148, 2447
BseMI GCAATG 2 cut(s) 228, 2292
BseMII CTCAG 4 cut(s) 58, 1139, 1359, 2418
BseNI ACTGG 8 cut(s) 105, 933, 1557, 1656, 2274, 2319, 2322, 2339
BseRI GAGGAG 1 cut(s) 1240
BseSI GKGCMC 2 cut(s) 605, 1810
BseXI GCAGC 7 cut(s) 232, 649, 1325, 1768, 1796, 1799, 2309
BseYI CCCAGC 3 cut(s) 1407, 2256, 2307
Bsh1236I CGCG 1 cut(s) 1306
Bsh1285I CGRYCG 1 cut(s) 2169
BshFI GGCC 9 cut(s) 603, 1103, 1217, 1808, 1955, 2060, 2068, 2072, 2490
BsiEI CGRYCG 1 cut(s) 2169
BsiHKAI GWGCWC 3 cut(s) 643, 1622, 1967
BsiHKCI CYCGRG 1 cut(s) 1609
BsiSI CCGG 5 cut(s) 320, 1091, 1104, 2069, 2169
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 1424, 1534
BslI CCNNNNNNNGG 4 cut(s) 319, 977, 2148, 2447
BsmAI GTCTC 4 cut(s) 130, 246, 1209, 2312
BsmFI GGGAC 2 cut(s) 1424, 1534
BsnI GGCC 9 cut(s) 603, 1103, 1217, 1808, 1955, 2060, 2068, 2072, 2490
Bso31I GGTCTC 1 cut(s) 2312
BsoBI CYCGRG 1 cut(s) 1609
Bsp119I TTCGAA 2 cut(s) 8, 230
Bsp120I GGGCCC 2 cut(s) 601, 1806
Bsp1286I GDGCHC 6 cut(s) 605, 643, 762, 1622, 1810, 1967
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 1115
Bsp143I GATC 3 cut(s) 1231, 1690, 1996
Bsp19I CCATGG 2 cut(s) 197, 1699
BspACI CCGC 3 cut(s) 1304, 1866, 2105
BspANI GGCC 9 cut(s) 603, 1103, 1217, 1808, 1955, 2060, 2068, 2072, 2490
BspCNI CTCAG 4 cut(s) 57, 1140, 1358, 2419
BspEI TCCGGA 1 cut(s) 1090
BspFNI CGCG 1 cut(s) 1306
BspLI GGNNCC 9 cut(s) 105, 160, 267, 373, 602, 603, 1808, 1894, 2452
BspMAI CTGCAG 2 cut(s) 667, 1786
BspMI ACCTGC 1 cut(s) 2264
BspOI GCTAGC 1 cut(s) 1407
BspPI GGATC 1 cut(s) 1226
BspQI GCTCTTC 3 cut(s) 399, 1627, 2115
BspT104I TTCGAA 2 cut(s) 8, 230
BspTI CTTAAG 1 cut(s) 2000
BspTNI GGTCTC 1 cut(s) 2312
BsrDI GCAATG 2 cut(s) 228, 2292
BsrFI RCCGGY 1 cut(s) 2068
BsrGI TGTACA 1 cut(s) 1115
BsrI ACTGG 8 cut(s) 105, 933, 1557, 1656, 2274, 2319, 2322, 2339
BssAI RCCGGY 1 cut(s) 2068
BssECI CCNNGG 3 cut(s) 197, 1699, 1978
BssMI GATC 3 cut(s) 1231, 1690, 1996
BssT1I CCWWGG 3 cut(s) 197, 1699, 1978
Bst2UI CCWGG 2 cut(s) 468, 1897
Bst6I CTCTTC 4 cut(s) 34, 399, 1627, 2115
BstAFI CTTAAG 1 cut(s) 2000
BstAPI GCANNNNNTGC 2 cut(s) 1652, 1796
BstAUI TGTACA 1 cut(s) 1115
BstBI TTCGAA 2 cut(s) 8, 230
BstC8I GCNNGC 5 cut(s) 758, 1405, 1864, 2070, 2202
BstDEI CTNAG 5 cut(s) 44, 1148, 1345, 2055, 2427
BstDSI CCRYGG 2 cut(s) 197, 1699
BstF5I GGATG 6 cut(s) 328, 584, 784, 826, 1657, 2219
BstFNI CGCG 1 cut(s) 1306
BstKTI GATC 3 cut(s) 1234, 1693, 1999
BstMAI GTCTC 4 cut(s) 130, 246, 1209, 2312
BstMBI GATC 3 cut(s) 1231, 1690, 1996
BstMCI CGRYCG 1 cut(s) 2169
BstNI CCWGG 2 cut(s) 468, 1897
BstNSI RCATGY 4 cut(s) 1330, 1588, 1748, 1884
BstSCI CCNGG 2 cut(s) 466, 1895
BstSFI CTRYAG 3 cut(s) 663, 1714, 1782
BstSLI GKGCMC 2 cut(s) 605, 1810
BstUI CGCG 1 cut(s) 1306
BstV1I GCAGC 7 cut(s) 232, 649, 1325, 1768, 1796, 1799, 2309
BstV2I GAAGAC 1 cut(s) 1488
BstXI CCANNNNNNTGG 1 cut(s) 474
BsuI GTATCC 1 cut(s) 1033
BsuRI GGCC 9 cut(s) 603, 1103, 1217, 1808, 1955, 2060, 2068, 2072, 2490
BtgI CCRYGG 2 cut(s) 197, 1699
BtsCI GGATG 6 cut(s) 328, 584, 784, 826, 1657, 2219
BtsI GCAGTG 3 cut(s) 79, 314, 416
BveI ACCTGC 1 cut(s) 2264
Cac8I GCNNGC 5 cut(s) 758, 1405, 1864, 2070, 2202
CaiI CAGNNNCTG 3 cut(s) 683, 917, 1680
Cfr10I RCCGGY 1 cut(s) 2068
Cfr13I GGNCC 7 cut(s) 104, 158, 503, 601, 602, 1806, 1807
CsiI ACCWGGT 1 cut(s) 1895
Csp6I GTAC 8 cut(s) 91, 582, 1116, 1507, 1975, 2012, 2267, 2312
CviQI GTAC 8 cut(s) 91, 582, 1116, 1507, 1975, 2012, 2267, 2312
DdeI CTNAG 5 cut(s) 44, 1148, 1345, 2055, 2427
DpnI GATC 3 cut(s) 1233, 1692, 1998
DpnII GATC 3 cut(s) 1231, 1690, 1996
DraI TTTAAA 1 cut(s) 39
DrdI GACNNNNNNGTC 1 cut(s) 1585
DseDI GACNNNNNNGTC 1 cut(s) 1585
EaeI YGGCCR 3 cut(s) 1215, 1953, 2070
Eam1104I CTCTTC 4 cut(s) 34, 399, 1627, 2115
EarI CTCTTC 4 cut(s) 34, 399, 1627, 2115
Eco130I CCWWGG 3 cut(s) 197, 1699, 1978
Eco24I GRGCYC 3 cut(s) 605, 762, 1810
Eco31I GGTCTC 1 cut(s) 2312
Eco47I GGWCC 3 cut(s) 104, 158, 503
Eco57I CTGAAG 6 cut(s) 118, 380, 538, 660, 894, 1121
Eco88I CYCGRG 1 cut(s) 1609
EcoO109I RGGNCCY 3 cut(s) 104, 601, 1807
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 466, 1895
EcoT14I CCWWGG 3 cut(s) 197, 1699, 1978
EcoT22I ATGCAT 1 cut(s) 1882
EcoT38I GRGCYC 3 cut(s) 605, 762, 1810
ErhI CCWWGG 3 cut(s) 197, 1699, 1978
FalI AAGNNNNNCTT 2 cut(s) 1606, 1638
FaqI GGGAC 2 cut(s) 1424, 1534
Fnu4HI GCNGC 8 cut(s) 246, 663, 1314, 1782, 1785, 1788, 2106, 2298
FokI GGATG 6 cut(s) 335, 591, 791, 833, 1664, 2226
FriOI GRGCYC 3 cut(s) 605, 762, 1810
FseI GGCCGGCC 1 cut(s) 2072
Fsp4HI GCNGC 8 cut(s) 246, 663, 1314, 1782, 1785, 1788, 2106, 2298
FspBI CTAG 7 cut(s) 338, 353, 1377, 1404, 1581, 1851, 1979
GluI GCNGC 8 cut(s) 246, 663, 1314, 1782, 1785, 1788, 2106, 2298
GsaI CCCAGC 3 cut(s) 1411, 2260, 2311
GsuI CTGGAG 5 cut(s) 112, 270, 450, 1338, 2336
HaeIII GGCC 9 cut(s) 603, 1103, 1217, 1808, 1955, 2060, 2068, 2072, 2490
HapII CCGG 5 cut(s) 320, 1091, 1104, 2069, 2169
HincII GTYRAC 4 cut(s) 328, 447, 1360, 2139
HindII GTYRAC 4 cut(s) 328, 447, 1360, 2139
HinfI GANTC 9 cut(s) 16, 344, 1033, 1211, 2172, 2404, 2431, 2464, 2474
HpaI GTTAAC 2 cut(s) 328, 1360
HpaII CCGG 5 cut(s) 320, 1091, 1104, 2069, 2169
HphI GGTGA 2 cut(s) 531, 1849
Hpy166II GTNNAC 8 cut(s) 79, 328, 447, 1262, 1360, 1975, 2014, 2139
Hpy188III TCNNGA 7 cut(s) 165, 1037, 1091, 1208, 1229, 2221, 2484
Hpy8I GTNNAC 8 cut(s) 79, 328, 447, 1262, 1360, 1975, 2014, 2139
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 9 cut(s) 562, 569, 918, 1145, 1511, 1608, 1813, 1820, 2389
HpyCH4IV ACGT 3 cut(s) 839, 1011, 1509
HpyF3I CTNAG 5 cut(s) 44, 1148, 1345, 2055, 2427
HpySE526I ACGT 3 cut(s) 839, 1011, 1509
Kpn2I TCCGGA 1 cut(s) 1090
KroI GCCGGC 1 cut(s) 2068
KroNI GCCGGC 1 cut(s) 2070
KspAI GTTAAC 2 cut(s) 328, 1360
Kzo9I GATC 3 cut(s) 1231, 1690, 1996
LguI GCTCTTC 3 cut(s) 399, 1627, 2115
LmnI GCTCC 7 cut(s) 361, 1021, 1106, 1310, 1454, 1527, 2450
Lsp1109I GCAGC 7 cut(s) 232, 649, 1325, 1768, 1796, 1799, 2309
LweI GCATC 4 cut(s) 1552, 1552, 1595, 1642
MabI ACCWGGT 1 cut(s) 1895
MaeI CTAG 7 cut(s) 338, 353, 1377, 1404, 1581, 1851, 1979
MaeII ACGT 3 cut(s) 839, 1011, 1509
MaeIII GTNAC 4 cut(s) 279, 653, 835, 1729
MalI GATC 3 cut(s) 1233, 1692, 1998
MboI GATC 3 cut(s) 1231, 1690, 1996
MhlI GDGCHC 6 cut(s) 605, 643, 762, 1622, 1810, 1967
MlyI GAGTC 3 cut(s) 1042, 2413, 2458
MmeI TCCRAC 1 cut(s) 1601
Mph1103I ATGCAT 1 cut(s) 1882
MroI TCCGGA 1 cut(s) 1090
MroNI GCCGGC 1 cut(s) 2068
MroXI GAANNNNTTC 1 cut(s) 264
MslI CAYNNNNRTG 4 cut(s) 750, 1743, 1881, 1970
MspA1I CMGCKG 2 cut(s) 1316, 2021
MspCI CTTAAG 1 cut(s) 2000
MspI CCGG 5 cut(s) 320, 1091, 1104, 2069, 2169
MspR9I CCNGG 2 cut(s) 468, 1897
MvaI CCWGG 2 cut(s) 468, 1897
MvnI CGCG 1 cut(s) 1306
NaeI GCCGGC 1 cut(s) 2070
NcoI CCATGG 2 cut(s) 197, 1699
NdeII GATC 3 cut(s) 1231, 1690, 1996
NgoMIV GCCGGC 1 cut(s) 2068
NheI GCTAGC 1 cut(s) 1403
NlaIV GGNNCC 9 cut(s) 105, 160, 267, 373, 602, 603, 1808, 1894, 2452
NsiI ATGCAT 1 cut(s) 1882
NspI RCATGY 4 cut(s) 1330, 1588, 1748, 1884
NspV TTCGAA 2 cut(s) 8, 230
OliI CACNNNNGTG 1 cut(s) 1970
PciSI GCTCTTC 3 cut(s) 399, 1627, 2115
PdiI GCCGGC 1 cut(s) 2070
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 6 cut(s) 16, 344, 1211, 2172, 2431, 2474
Pfl23II CGTACG 1 cut(s) 90
PflFI GACNNNGTC 1 cut(s) 1487
PflMI CCANNNNNTGG 1 cut(s) 2447
PfoI TCCNGGA 1 cut(s) 466
PkrI GCNGC 8 cut(s) 247, 664, 1315, 1783, 1786, 1789, 2107, 2299
PleI GAGTC 3 cut(s) 1041, 2412, 2458
PpsI GAGTC 3 cut(s) 1041, 2412, 2458
PpuMI RGGWCCY 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 531
Psp1406I AACGTT 2 cut(s) 839, 1011
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 466, 1895
PspFI CCCAGC 3 cut(s) 1407, 2256, 2307
PspGI CCWGG 2 cut(s) 466, 1895
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 9 cut(s) 105, 160, 267, 373, 602, 603, 1808, 1894, 2452
PspOMI GGGCCC 2 cut(s) 601, 1806
PspPI GGNCC 7 cut(s) 104, 158, 503, 601, 602, 1806, 1807
PspPPI RGGWCCY 1 cut(s) 104
PstI CTGCAG 2 cut(s) 667, 1786
PstNI CAGNNNCTG 3 cut(s) 683, 917, 1680
PsyI GACNNNGTC 1 cut(s) 1487
PvuII CAGCTG 2 cut(s) 1316, 2021
RigI GGCCGGCC 1 cut(s) 2072
RsaI GTAC 8 cut(s) 92, 583, 1117, 1508, 1976, 2013, 2268, 2313
RsaNI GTAC 8 cut(s) 91, 582, 1116, 1507, 1975, 2012, 2267, 2312
RseI CAYNNNNRTG 4 cut(s) 750, 1743, 1881, 1970
SapI GCTCTTC 3 cut(s) 399, 1627, 2115
SatI GCNGC 8 cut(s) 246, 663, 1314, 1782, 1785, 1788, 2106, 2298
Sau3AI GATC 3 cut(s) 1231, 1690, 1996
Sau96I GGNCC 7 cut(s) 104, 158, 503, 601, 602, 1806, 1807
SchI GAGTC 3 cut(s) 1042, 2413, 2458
ScrFI CCNGG 2 cut(s) 468, 1897
SduI GDGCHC 6 cut(s) 605, 643, 762, 1622, 1810, 1967
SexAI ACCWGGT 1 cut(s) 1895
SfaNI GCATC 4 cut(s) 1552, 1552, 1595, 1642
SfcI CTRYAG 3 cut(s) 663, 1714, 1782
SfuI TTCGAA 2 cut(s) 8, 230
SinI GGWCC 3 cut(s) 104, 158, 503
SmiI ATTTAAAT 1 cut(s) 39
SmiMI CAYNNNNRTG 4 cut(s) 750, 1743, 1881, 1970
SmlI CTYRAG 2 cut(s) 2000, 2219
SmoI CTYRAG 2 cut(s) 2000, 2219
SsiI CCGC 3 cut(s) 1304, 1866, 2105
SspI AATATT 1 cut(s) 2077
SspMI CTAG 7 cut(s) 338, 353, 1377, 1404, 1581, 1851, 1979
StyD4I CCNGG 2 cut(s) 466, 1895
StyI CCWWGG 3 cut(s) 197, 1699, 1978
SwaI ATTTAAAT 1 cut(s) 39
TaiI ACGT 3 cut(s) 842, 1014, 1512
TatI WGTACW 3 cut(s) 1115, 2011, 2266
TauI GCSGC 1 cut(s) 2108
TfiI GAWTC 6 cut(s) 16, 344, 1211, 2172, 2431, 2474
TseI GCWGC 7 cut(s) 245, 662, 1313, 1781, 1784, 1787, 2297
TspDTI ATGAA 7 cut(s) 708, 1365, 1380, 1918, 1939, 2466, 2472
Tth111I GACNNNGTC 1 cut(s) 1487
Van91I CCANNNNNTGG 1 cut(s) 2447
Vha464I CTTAAG 1 cut(s) 2000
VpaK11BI GGWCC 3 cut(s) 104, 158, 503
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 3 cut(s) 10, 430, 807
XceI RCATGY 4 cut(s) 1330, 1588, 1748, 1884
XcmI CCANNNNNNNNNTGG 2 cut(s) 568, 1275
XmaJI CCTAGG 1 cut(s) 1978
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 7 cut(s) 338, 353, 1377, 1404, 1581, 1851, 1979
Zsp2I ATGCAT 1 cut(s) 1882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.