pycom01g09620

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003810
Physical Location & Seq
Reverse (-)
10460186 .. 10464446
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09620.1

Sequence Viewer

Length: 2085 bp
ATGCACCTGAAACCATTAGTTCCCTGTTTACCGTATGTGATGAAGTTTGTTATTAATCGCGCCCCATCATACTCATTCAGTGCCAATATCACTGTGGAAGGATGTACGACCCTAACCATGTGGGGCCCATACTGCAATCAGACAATTAACACACTTTCATGTGCTCAATCTAACAGTTACAGTGCTGCAGACAATACTTCAGAAGCTGGTTTGTATAACCAAACAATTGAATATGTAATTTCATGTAAAAACAATTTTGGCACTTGTCATGGAGATGGTGAGCCCAAATTCTACTCTTTGGATGTAGTGGGAGTATCACACGAGTTGAAAATTTTGGCGAAGGATGTTTGGTTAAATGTAACATCTTCAGATAACACCAAGAATGTTAGTGATATTAATTTAATATTTTATGCACGGAATGGTGCAATACCTTCAGAAACTGTGCATGACTATTCCAGTAATATAAAAAAATCCTCATTGGTCATTCAATTTCCAAAGGTCGGTCGATGGTATATTACTGTTTTACCTCTCAACATTTCAAAAGAGCTTGAAGGGAGTCTGGATACTGACACAAAAGTTTGTTATTCCTTGGAATCAAAACTGCTTGAATGTCCGGAGGGCAAGGCCGGAGCAAATTGTACATGGGAAATGTACACCCTTCAGGTGGGCTTGCTGGTAAATGTGTTTCTGCTTATGAAAGTTCTTGAAACTGAGAATGGTTGTCTGCAGACATCGCTGAGGAAAGGTTCAAGCTACTTTGAATACTACTATTTACTCATCAGTGAAAAAGTGCCTCCTGAGTCAGCCAATTTTCCTCTTGATCATCTTTTAACCAACTCCTCGCATGGTGGACAACCAGAAGAAACATGGACTTACTTTATGTTGGACATTCCGCGTGGAGCAGCTGGAGGACATATGCACATTCAATTAAACTCCGATGACAAGGTTAACTATGAAGTATATGCTAGATATGGTGGATTACCATCTCTTGCTAGCTGGGACTATTTTTATTCGAACAGGACAAGCAGTAGCCATGGCTCCATGTTTTTTAACTTATATAATTCAAGTGAAGACAAGGTTGATTTTTACATTTTGTACATTAGAGAAGGAACTTGGGGTTTTGGTCTAAAGCATCTCAACACCACCAGTGATGCTTCGAAAGTTCCGACTATCATGTCTGTTTCCCTTGAAAGATGCCCGAGAAGGTGCTCTTCACATGGAAAATGCGAAACTGCGTTGGACGCTAGTGGATTGGCATCATACAGCTTCTGCTCATGTGATCCAGACCATGGAGGCTTTGACTGTAGCATTGAACTTGTAACACCTCATGGGCACGTATGGCAATCCATTTTTCTTATTGCGTCAAATGCTGCAGCAGCACTTCCTGCATTTTGGGCCCTTCGACAGAAGGCATTGGCAGAGTGGGTGATATTTACATCTAGTGGAATTGCAAGCGGAATATATCATGCATGTGATGTGGGAACTTGGTGTCCTCTGGGCTTTGGTGTCTTACAGTTCATGGACGTCTGGCTTTCTTTCATGGCCGTAGGGAGCACATTTGTGTACCTAGGCACAGTTGAGGACAATCTTAAGAGAGCATTACACACAGCCGTTGCACTTCTTACTGCACTCATGGTTTATACTAAGGCCAGAAGGCCGGGGAATATTATTCTTGTTATAGTAATTGGAACCATTGCTCTTCTTATTGGGTGGCTGATAGAATTGTCAACCAAGTTTAGGTCATTTTCATTTTCGACCAGATTCTCCTTAAATATGCTTGAGAGGCTCCAAGCTGTTAACGGATGGCTGAAGAATCTCATTAAGACAACTTTTAGAAGATTTCGCTGGGGTTTTATACTTGCAGGTTTAGTTGCATTAGCCATTGCAGCAAGTAGCTGGGTACTGGAAACCAATGAAAGCTACTGGATTTGGCACAGCATTTGGCACATGACAATATATACGTCTTCCTTCTTCTTCCTATGCTCAAAAGAAAGTCCTACAAGTACGGTAGATACTGAGAGTCAGATACCACCGAATGGAGCCTATGAATTGACTCGACAAGATTCATTTTCAAGAGGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

695

Amino Acids

77.47

Weight (kDa)

6.07

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 445 - 650 9.3e-42 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1174
AatII GACGTC 1 cut(s) 1527
Acc36I ACCTGC 1 cut(s) 1853
AccB7I CCANNNNNTGG 2 cut(s) 1289, 2036
AccII CGCG 2 cut(s) 60, 895
AccIII TCCGGA 1 cut(s) 613
AciI CCGC 2 cut(s) 893, 1455
AclWI GGATC 1 cut(s) 1274
AcoI YGGCCR 1 cut(s) 1542
AcsI RAATTY 2 cut(s) 287, 330
AcuI CTGAAG 5 cut(s) 183, 351, 417, 644, 1829
AcyI GRCGYC 1 cut(s) 1524
AfaI GTAC 7 cut(s) 106, 640, 653, 1097, 1565, 1902, 2005
AfiI CCNNNNNNNGG 5 cut(s) 500, 664, 1289, 1737, 2036
AflII CTTAAG 1 cut(s) 1589
AleI CACNNNNGTG 1 cut(s) 1559
AloI GAACNNNNNNTCC 2 cut(s) 1474, 1506
AluBI AGCT 9 cut(s) 206, 547, 753, 905, 996, 1266, 1793, 1896, 1920
AluI AGCT 9 cut(s) 206, 547, 753, 905, 996, 1266, 1793, 1896, 1920
Alw21I GWGCWC 3 cut(s) 166, 1211, 1556
AlwI GGATC 1 cut(s) 1274
AlwNI CAGNNNCTG 3 cut(s) 206, 440, 1269
Ama87I CYCGRG 1 cut(s) 1198
Aor13HI TCCGGA 1 cut(s) 613
AoxI GGCC 6 cut(s) 124, 624, 1395, 1542, 1647, 1655
ApaI GGGCCC 2 cut(s) 128, 1399
ApeKI GCWGC 6 cut(s) 185, 902, 1370, 1373, 1376, 1886
ApoI RAATTY 2 cut(s) 287, 330
AseI ATTAAT 2 cut(s) 54, 396
AspA2I CCTAGG 1 cut(s) 1567
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 4 cut(s) 124, 125, 1395, 1396
AsuC2I CCSGG 1 cut(s) 1659
AsuHPI GGTGA 2 cut(s) 290, 1438
AsuII TTCGAA 2 cut(s) 1013, 1157
AsuNHI GCTAGC 1 cut(s) 992
AvaI CYCGRG 1 cut(s) 1198
AvrII CCTAGG 1 cut(s) 1567
BaeGI GKGCMC 3 cut(s) 128, 1335, 1399
BanII GRGCYC 3 cut(s) 128, 285, 1399
BauI CACGAG 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 1077, 1956
Bbv12I GWGCWC 3 cut(s) 166, 1211, 1556
BbvCI CCTCAGC 1 cut(s) 737
BbvI GCAGC 6 cut(s) 172, 914, 1357, 1385, 1388, 1898
BccI CCATC 5 cut(s) 73, 269, 501, 991, 1797
BceAI ACGGC 2 cut(s) 1529, 1595
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 1659
BfaI CTAG 5 cut(s) 966, 993, 1245, 1440, 1568
BfmI CTRYAG 4 cut(s) 186, 725, 1303, 1371
BfrI CTTAAG 1 cut(s) 1589
BfuAI ACCTGC 1 cut(s) 1853
BfuI GTATCC 1 cut(s) 556
BisI GCNGC 6 cut(s) 186, 903, 1371, 1374, 1377, 1887
BlnI CCTAGG 1 cut(s) 1567
BlsI GCNGC 6 cut(s) 187, 904, 1372, 1375, 1378, 1888
Bme1390I CCNGG 1 cut(s) 1659
BmeT110I CYCGRG 1 cut(s) 1198
BmgT120I GGNCC 4 cut(s) 124, 125, 1395, 1396
BmiI GGNNCC 7 cut(s) 125, 126, 1039, 1397, 1690, 1787, 2041
BmrFI CCNGG 1 cut(s) 1659
BmsI GCATC 4 cut(s) 1141, 1141, 1184, 1265
BmtI GCTAGC 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 1077, 1956
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 737
Bpu14I TTCGAA 2 cut(s) 1013, 1157
BpuEI CTTGAG 1 cut(s) 1799
BpuMI CCSGG 1 cut(s) 1659
BsaAI YACGTR 1 cut(s) 1336
BsaBI GATNNNNATC 2 cut(s) 982, 1255
BsaHI GRCGYC 1 cut(s) 1524
BsaJI CCNNGG 5 cut(s) 588, 1033, 1288, 1567, 1658
BsaWI WCCGGW 1 cut(s) 613
Bsc4I CCNNNNNNNGG 5 cut(s) 500, 664, 1289, 1737, 2036
Bse1I ACTGG 4 cut(s) 456, 1146, 1908, 1928
Bse3DI GCAATG 2 cut(s) 1692, 1881
Bse8I GATNNNNATC 2 cut(s) 982, 1255
BseAI TCCGGA 1 cut(s) 613
BseDI CCNNGG 5 cut(s) 588, 1033, 1288, 1567, 1658
BseGI GGATG 4 cut(s) 107, 307, 349, 1808
BseJI GATNNNNATC 2 cut(s) 982, 1255
BseLI CCNNNNNNNGG 5 cut(s) 500, 664, 1289, 1737, 2036
BseMI GCAATG 2 cut(s) 1692, 1881
BseMII CTCAG 4 cut(s) 702, 728, 789, 2007
BseNI ACTGG 4 cut(s) 456, 1146, 1908, 1928
BseRI GAGGAG 1 cut(s) 829
BseSI GKGCMC 3 cut(s) 128, 1335, 1399
BseXI GCAGC 6 cut(s) 172, 914, 1357, 1385, 1388, 1898
BseYI CCCAGC 3 cut(s) 996, 1845, 1896
BsgI GTGCAG 1 cut(s) 1611
Bsh1236I CGCG 2 cut(s) 60, 895
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 6 cut(s) 126, 626, 1397, 1544, 1649, 1657
BsiEI CGRYCG 1 cut(s) 505
BsiHKAI GWGCWC 3 cut(s) 166, 1211, 1556
BsiHKCI CYCGRG 1 cut(s) 1198
BsiSI CCGG 3 cut(s) 614, 627, 1658
BslFI GGGAC 1 cut(s) 1013
BslI CCNNNNNNNGG 5 cut(s) 500, 664, 1289, 1737, 2036
BsmFI GGGAC 1 cut(s) 1013
BsnI GGCC 6 cut(s) 126, 626, 1397, 1544, 1649, 1657
BsoBI CYCGRG 1 cut(s) 1198
Bsp119I TTCGAA 2 cut(s) 1013, 1157
Bsp120I GGGCCC 2 cut(s) 124, 1395
Bsp1286I GDGCHC 7 cut(s) 128, 166, 285, 1211, 1335, 1399, 1556
Bsp13I TCCGGA 1 cut(s) 613
Bsp1407I TGTACA 3 cut(s) 638, 651, 1095
Bsp143I GATC 2 cut(s) 820, 1279
Bsp19I CCATGG 2 cut(s) 1033, 1288
BspACI CCGC 2 cut(s) 893, 1455
BspANI GGCC 6 cut(s) 126, 626, 1397, 1544, 1649, 1657
BspCNI CTCAG 4 cut(s) 703, 729, 790, 2008
BspEI TCCGGA 1 cut(s) 613
BspFNI CGCG 2 cut(s) 60, 895
BspLI GGNNCC 7 cut(s) 125, 126, 1039, 1397, 1690, 1787, 2041
BspMAI CTGCAG 3 cut(s) 190, 729, 1375
BspMI ACCTGC 1 cut(s) 1853
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 1 cut(s) 1274
BspQI GCTCTTC 2 cut(s) 1216, 1704
BspT104I TTCGAA 2 cut(s) 1013, 1157
BspTI CTTAAG 1 cut(s) 1589
BsrDI GCAATG 2 cut(s) 1692, 1881
BsrGI TGTACA 3 cut(s) 638, 651, 1095
BsrI ACTGG 4 cut(s) 456, 1146, 1908, 1928
BssECI CCNNGG 5 cut(s) 588, 1033, 1288, 1567, 1658
BssMI GATC 2 cut(s) 820, 1279
BssNI GRCGYC 1 cut(s) 1524
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 4 cut(s) 588, 1033, 1288, 1567
Bst2BI CACGAG 1 cut(s) 320
Bst6I CTCTTC 2 cut(s) 1216, 1704
BstACI GRCGYC 1 cut(s) 1524
BstAFI CTTAAG 1 cut(s) 1589
BstAPI GCANNNNNTGC 1 cut(s) 1385
BstAUI TGTACA 3 cut(s) 638, 651, 1095
BstBAI YACGTR 1 cut(s) 1336
BstBI TTCGAA 2 cut(s) 1013, 1157
BstC8I GCNNGC 3 cut(s) 671, 994, 1453
BstDEI CTNAG 5 cut(s) 711, 737, 798, 1644, 2016
BstDSI CCRYGG 2 cut(s) 1033, 1288
BstF5I GGATG 4 cut(s) 107, 307, 349, 1808
BstFNI CGCG 2 cut(s) 60, 895
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 2 cut(s) 823, 1282
BstMBI GATC 2 cut(s) 820, 1279
BstMCI CGRYCG 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 1473
BstSCI CCNGG 1 cut(s) 1657
BstSFI CTRYAG 4 cut(s) 186, 725, 1303, 1371
BstSLI GKGCMC 3 cut(s) 128, 1335, 1399
BstUI CGCG 2 cut(s) 60, 895
BstV1I GCAGC 6 cut(s) 172, 914, 1357, 1385, 1388, 1898
BstV2I GAAGAC 2 cut(s) 1077, 1956
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 6 cut(s) 126, 626, 1397, 1544, 1649, 1657
BtgI CCRYGG 2 cut(s) 1033, 1288
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 4 cut(s) 107, 307, 349, 1808
BtsIMutI CAGTG 5 cut(s) 85, 90, 187, 787, 1153
BveI ACCTGC 1 cut(s) 1853
Cac8I GCNNGC 3 cut(s) 671, 994, 1453
CaiI CAGNNNCTG 3 cut(s) 206, 440, 1269
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 4 cut(s) 124, 125, 1395, 1396
CseI GACGC 2 cut(s) 1250, 1350
Csp6I GTAC 7 cut(s) 105, 639, 652, 1096, 1564, 1901, 2004
CviQI GTAC 7 cut(s) 105, 639, 652, 1096, 1564, 1901, 2004
DdeI CTNAG 5 cut(s) 711, 737, 798, 1644, 2016
DpnI GATC 2 cut(s) 822, 1281
DpnII GATC 2 cut(s) 820, 1279
DrdI GACNNNNNNGTC 1 cut(s) 1174
DseDI GACNNNNNNGTC 1 cut(s) 1174
EaeI YGGCCR 1 cut(s) 1542
Eam1104I CTCTTC 2 cut(s) 1216, 1704
EarI CTCTTC 2 cut(s) 1216, 1704
Eco130I CCWWGG 4 cut(s) 588, 1033, 1288, 1567
Eco24I GRGCYC 3 cut(s) 128, 285, 1399
Eco57I CTGAAG 5 cut(s) 183, 351, 417, 644, 1829
Eco88I CYCGRG 1 cut(s) 1198
EcoO109I RGGNCCY 2 cut(s) 124, 1396
EcoT14I CCWWGG 4 cut(s) 588, 1033, 1288, 1567
EcoT22I ATGCAT 1 cut(s) 1471
EcoT38I GRGCYC 3 cut(s) 128, 285, 1399
ErhI CCWWGG 4 cut(s) 588, 1033, 1288, 1567
FalI AAGNNNNNCTT 2 cut(s) 1195, 1227
FaqI GGGAC 1 cut(s) 1013
FauNDI CATATG 1 cut(s) 915
FbaI TGATCA 1 cut(s) 820
Fnu4HI GCNGC 6 cut(s) 186, 903, 1371, 1374, 1377, 1887
FokI GGATG 4 cut(s) 114, 314, 356, 1815
FriOI GRGCYC 3 cut(s) 128, 285, 1399
Fsp4HI GCNGC 6 cut(s) 186, 903, 1371, 1374, 1377, 1887
FspBI CTAG 5 cut(s) 966, 993, 1245, 1440, 1568
GlaI GCGC 1 cut(s) 61
GluI GCNGC 6 cut(s) 186, 903, 1371, 1374, 1377, 1887
GsaI CCCAGC 3 cut(s) 1000, 1849, 1900
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 6 cut(s) 126, 626, 1397, 1544, 1649, 1657
HapII CCGG 3 cut(s) 614, 627, 1658
HgaI GACGC 2 cut(s) 1250, 1350
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 1524
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 3 cut(s) 949, 1728, 1798
HindII GTYRAC 3 cut(s) 949, 1728, 1798
HinfI GANTC 8 cut(s) 556, 593, 800, 1761, 1813, 2020, 2053, 2063
HpaI GTTAAC 2 cut(s) 949, 1798
HpaII CCGG 3 cut(s) 614, 627, 1658
HphI GGTGA 2 cut(s) 290, 1438
Hpy166II GTNNAC 7 cut(s) 29, 654, 851, 949, 1564, 1728, 1798
Hpy188I TCNGA 7 cut(s) 141, 202, 370, 436, 937, 1167, 2025
Hpy188III TCNNGA 7 cut(s) 560, 614, 704, 797, 818, 1283, 2073
Hpy8I GTNNAC 7 cut(s) 29, 654, 851, 949, 1564, 1728, 1798
HpyCH4IV ACGT 3 cut(s) 1335, 1524, 1961
HpyF3I CTNAG 5 cut(s) 711, 737, 798, 1644, 2016
HpySE526I ACGT 3 cut(s) 1335, 1524, 1961
Hsp92I GRCGYC 1 cut(s) 1524
HspAI GCGC 1 cut(s) 60
Kpn2I TCCGGA 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 820
KspAI GTTAAC 2 cut(s) 949, 1798
Kzo9I GATC 2 cut(s) 820, 1279
LguI GCTCTTC 2 cut(s) 1216, 1704
LmnI GCTCC 6 cut(s) 629, 899, 1043, 1551, 1791, 2039
Lsp1109I GCAGC 6 cut(s) 172, 914, 1357, 1385, 1388, 1898
LweI GCATC 4 cut(s) 1141, 1141, 1184, 1265
MaeI CTAG 5 cut(s) 966, 993, 1245, 1440, 1568
MaeII ACGT 3 cut(s) 1335, 1524, 1961
MaeIII GTNAC 3 cut(s) 176, 358, 1318
MalI GATC 2 cut(s) 822, 1281
MboI GATC 2 cut(s) 820, 1279
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 7 cut(s) 128, 166, 285, 1211, 1335, 1399, 1556
MlyI GAGTC 4 cut(s) 565, 809, 2029, 2047
MmeI TCCRAC 3 cut(s) 864, 1190, 1218
Mph1103I ATGCAT 1 cut(s) 1471
MroI TCCGGA 1 cut(s) 613
MslI CAYNNNNRTG 4 cut(s) 157, 273, 1470, 1559
MspA1I CMGCKG 1 cut(s) 905
MspCI CTTAAG 1 cut(s) 1589
MspI CCGG 3 cut(s) 614, 627, 1658
MspR9I CCNGG 1 cut(s) 1659
MunI CAATTG 1 cut(s) 225
MvnI CGCG 2 cut(s) 60, 895
NciI CCSGG 1 cut(s) 1659
NcoI CCATGG 2 cut(s) 1033, 1288
NdeI CATATG 1 cut(s) 915
NdeII GATC 2 cut(s) 820, 1279
NheI GCTAGC 1 cut(s) 992
NlaIV GGNNCC 7 cut(s) 125, 126, 1039, 1397, 1690, 1787, 2041
NsiI ATGCAT 1 cut(s) 1471
NspI RCATGY 1 cut(s) 1473
NspV TTCGAA 2 cut(s) 1013, 1157
OliI CACNNNNGTG 1 cut(s) 1559
PciSI GCTCTTC 2 cut(s) 1216, 1704
PcsI WCGNNNNNNNCGW 1 cut(s) 1163
PfeI GAWTC 4 cut(s) 593, 1761, 1813, 2063
PflMI CCANNNNNTGG 2 cut(s) 1289, 2036
PkrI GCNGC 6 cut(s) 187, 904, 1372, 1375, 1378, 1888
PleI GAGTC 4 cut(s) 564, 808, 2028, 2047
PpsI GAGTC 4 cut(s) 564, 808, 2028, 2047
Ppu21I YACGTR 1 cut(s) 1336
PshBI ATTAAT 2 cut(s) 54, 396
PspFI CCCAGC 3 cut(s) 996, 1845, 1896
PspN4I GGNNCC 7 cut(s) 125, 126, 1039, 1397, 1690, 1787, 2041
PspOMI GGGCCC 2 cut(s) 124, 1395
PspPI GGNCC 4 cut(s) 124, 125, 1395, 1396
PstI CTGCAG 3 cut(s) 190, 729, 1375
PstNI CAGNNNCTG 3 cut(s) 206, 440, 1269
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 7 cut(s) 106, 640, 653, 1097, 1565, 1902, 2005
RsaNI GTAC 7 cut(s) 105, 639, 652, 1096, 1564, 1901, 2004
RseI CAYNNNNRTG 4 cut(s) 157, 273, 1470, 1559
SapI GCTCTTC 2 cut(s) 1216, 1704
SatI GCNGC 6 cut(s) 186, 903, 1371, 1374, 1377, 1887
Sau3AI GATC 2 cut(s) 820, 1279
Sau96I GGNCC 4 cut(s) 124, 125, 1395, 1396
SchI GAGTC 4 cut(s) 565, 809, 2029, 2047
ScrFI CCNGG 1 cut(s) 1659
SduI GDGCHC 7 cut(s) 128, 166, 285, 1211, 1335, 1399, 1556
SfaNI GCATC 4 cut(s) 1141, 1141, 1184, 1265
SfcI CTRYAG 4 cut(s) 186, 725, 1303, 1371
SfuI TTCGAA 2 cut(s) 1013, 1157
SmiMI CAYNNNNRTG 4 cut(s) 157, 273, 1470, 1559
SmlI CTYRAG 2 cut(s) 1589, 1778
SmoI CTYRAG 2 cut(s) 1589, 1778
SsiI CCGC 2 cut(s) 893, 1455
SspI AATATT 2 cut(s) 405, 1666
SspMI CTAG 5 cut(s) 966, 993, 1245, 1440, 1568
StyD4I CCNGG 1 cut(s) 1657
StyI CCWWGG 4 cut(s) 588, 1033, 1288, 1567
TaiI ACGT 3 cut(s) 1338, 1527, 1964
TaqI TCGA 6 cut(s) 505, 1013, 1157, 1402, 1754, 2056
TaqII GACCGA 1 cut(s) 491
TatI WGTACW 3 cut(s) 638, 651, 1095
TfiI GAWTC 4 cut(s) 593, 1761, 1813, 2063
TscAI CASTG 5 cut(s) 85, 97, 187, 787, 1153
TseI GCWGC 6 cut(s) 185, 902, 1370, 1373, 1376, 1886
TspGWI ACGGA 2 cut(s) 430, 1815
TspRI CASTG 5 cut(s) 85, 97, 187, 787, 1153
Van91I CCANNNNNTGG 2 cut(s) 1289, 2036
Vha464I CTTAAG 1 cut(s) 1589
VspI ATTAAT 2 cut(s) 54, 396
XapI RAATTY 2 cut(s) 287, 330
XceI RCATGY 1 cut(s) 1473
XcmI CCANNNNNNNNNTGG 2 cut(s) 91, 864
XmaJI CCTAGG 1 cut(s) 1567
XspI CTAG 5 cut(s) 966, 993, 1245, 1440, 1568
ZraI GACGTC 1 cut(s) 1525
Zsp2I ATGCAT 1 cut(s) 1471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.