Rw1G023470

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003810
Physical Location & Seq
Reverse (-)
49267140 .. 49274370
7231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023470.1

Sequence Viewer

Length: 2487 bp
ATGGCTTCAAATTCGATTCCTTGCTCCTCTCCCTCTATGAATCTCGGTCTTTTCCTTCTTTTTATATCCTTATTCGGACACTGTTATTCCTACGACGAACTGGGTCCTTCCACAAACAGCTTCACAGTCTCCAGCTTCAGCTACCCACCTACAGTGCTCAAGAACTCTAATTTTCACTATATCAGAGTTGAACTGCCACCATGGTTCTCTTCAATGTCTATAGCAATGAACTCGAACGTAGACCTAGGTCATGTAAACATAGAAAAGGTTCCTAAAATTACAATGCCTTTGATTTGCTTCAGGGAGGGTAGCCCTCCACTGCCCGATGTCAATACCTCTCTAACAGATTCGGTTTTAGTTCCCTTTTACGATGGTTCACTTAAGGCAACACAAGCTCTTCAGAGTGCAGAGCAATGCTATCTTATGCAAAAGAATTTCACAGTGAAGTTGACAAATGAGCAGATTTCTCCAGGAGTTTGGTATTTGGGTCTTTTCAATGGTATTGGACCTACAAGGACGCAATCAAAAATGATTAACCGTGGATCTCAATATACCTTCAGTGCCAACATCACAGTTGAAGGATGTACAACTGAAACCATGTGGGGCCAGTATTGCAACCAGACAATTTATCCTCTTTTTTGTTCTCTATCTGGCAGCTACAATCCTGCAGAAAATTCTTCAGAGGCTAATTTGTACAGCAGTACAATGGGTTATGGTATTTCTTGCAAAAATGATTTTGAAACTTCTTGTCAAGGAGATGGGGAACCAAAGTTTTACTCTTTGGATGTGGTGGGAATATCACAGGAGTTAAACATTGTGGCAATGGATGTTTGGTTAAATGAAACGTTATCAAATAACACTAAGAATGGTAGTGATATTAATTTAATGTGTTTTGCACGTCACGGCGCAATACCTTCAGAATCTCTGCATGATTATTCCAGTAATATAAACAATAATCCTTTGGTCATTAACTTTCCAAAGGTTGGCCGCTGGTATATTACTGTTTTACCTGTCAACCTCTCAAAAGTGCTTGGAGGGAGTTTAGATACTTCCATAAAAGTTTGCTACTCCATGGAATCAAAACTGCTTGAATGTCCACTGGGGAAGGCTGGAGCAAATTGTACATGGGAAACATACACCCTTCAGACGGTTCTGAGGAAAGGTTCAGGCTTCTTTGAGTCCTACTATTTGCCTGACAGTGGAAAAGTATCTTCTGACTCAGCCAATTTTCCTCTTGATTCAATTGTAACCAACACATCGATTGGCGAAAAACCAGATAATACCTGGACTTATTTTGTTCTCGACATTCCTCGTGGTGCAGCTGGAGGAAATATGCACATTCGATTAACATCAGATACGAAGATTAAGTACGAAGTATATACTAGATTTGGTGGATTACCATCTCTTACCAGCTGGGACTACTATTATGCTAACAGGACAAGCAATAGTGTTGGCTCCATGTTTTTCAACTTATTTAACTCTAGTGAAGACAAGGTTGATTTTTACGTGTTTTACGTCAGAGAAGGAACTTGGGGTTTTGGACTAAAGCATCTAGACGCCACCAGTGTTGCTTCCAAAGTTCAAACTACCATGTCTCTTTCCCTTGAAAGATGCCCTAGAAGATGCTCCTCACATGGAAAATGCGAAAATGACTTAGATGCTAGTGGATTAACATCATACAGCTACTGCTCATGTGACCGGGACCATGGAGGCTTTGATTGTAGTATTGAAATTGTATCTCATCATGGGCACATATGGCAATCAATTTTTCTTATCGCGTCAAATGCTGCAGCAGCATTTCCTGCCTTCTATTCCCTTCGACAGAAGGCATGGTCAGAATGGGTGATATATACATCTAGTGGAATTGCAAGTGGAATTTATCATGCCTGTGATGTGGGAACATGGTGTCCTCTGGATTTTCATACTTTACAGTTCATGGACTTCTGGCTGTCTTTCATGGCCGTAGTGAGCACATTTGTGTACCTAGCTACAATGGATGAAGCTCATAAGAGAGCAGTCCTCACAGCTGTAGCAATTTTTACTGCCCTTATGGCAGTTACTAAGGCCACAAGGACGTCCAATATTGCGATTGTAATGTCAATTGGCATTTCGGCTCTTCTTCTTGGATGGCTGATAGAATTAACAACCAAGTATAGGTCATTTTCCTTTCCGGTTGGATTCTCCTTAAATATAATTGAGAGGTTCCAAGGTGTTAGAGGATGGCTCAAAGATCTTATTAAGACAGTTTTCAAAAGATTTCGCTGGGGCTTTGTACTCGCAGGTTCTACTGCATTAGCCATGGCAGCAATCAGCCGGACACTGGAGAGCACTGAAACCTACTGGATTTGGCACAGCCTTTGGCATGTCACCATATATACGTCTTCCTTCTTCTTCCTTTGCTCAAAAGCAAGTACTGAGAGTACTGAAAATACTACCCCAAATGGAGCCTATGAGTTGACTAGGCAGGATTCATTTCCAAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

828

Amino Acids

92.16

Weight (kDa)

6.04

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 584 - 789 1.1e-38 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2078
Acc36I ACCTGC 1 cut(s) 2270
AccB7I CCANNNNNTGG 1 cut(s) 983
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 1778
AciI CCGC 1 cut(s) 988
AclI AACGTT 1 cut(s) 845
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 2 cut(s) 985, 1959
AcsI RAATTY 4 cut(s) 10, 433, 673, 1875
AcuI CTGAAG 7 cut(s) 121, 283, 383, 541, 663, 900, 1127
AcyI GRCGYC 2 cut(s) 1557, 2075
AfaI GTAC 9 cut(s) 586, 695, 703, 1123, 1370, 1982, 2274, 2413, 2422
AfiI CCNNNNNNNGG 5 cut(s) 983, 1147, 1199, 2154, 2320
AflII CTTAAG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 1506
AjiI CACGTC 1 cut(s) 899
AjnI CCWGG 2 cut(s) 469, 1283
AleI CACNNNNGTG 1 cut(s) 1976
AloI GAACNNNNNNTCC 2 cut(s) 1891, 1923
Alw21I GWGCWC 3 cut(s) 159, 1973, 2330
Alw26I GTCTC 2 cut(s) 133, 1599
AlwI GGATC 1 cut(s) 550
AlwNI CAGNNNCTG 1 cut(s) 1686
AoxI GGCC 4 cut(s) 604, 985, 1959, 2064
ApeKI GCWGC 6 cut(s) 654, 1319, 1787, 1790, 1793, 2303
ApoI RAATTY 4 cut(s) 10, 433, 673, 1875
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 267
AspA2I CCTAGG 1 cut(s) 244
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 4 cut(s) 104, 506, 604, 1702
AsuC2I CCSGG 1 cut(s) 1700
AsuHPI GGTGA 2 cut(s) 1855, 2359
AvaII GGWCC 3 cut(s) 104, 506, 1702
AvrII CCTAGG 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 1752
BarI GAAGNNNNNNTAC 2 cut(s) 1352, 1384
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 2 cut(s) 1494, 2373
Bbv12I GWGCWC 3 cut(s) 159, 1973, 2330
BbvI GCAGC 6 cut(s) 666, 1331, 1774, 1802, 1805, 2315
BccI CCATC 5 cut(s) 365, 752, 1408, 2121, 2214
BceAI ACGGC 2 cut(s) 919, 1946
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 2 cut(s) 471, 1285
BcnI CCSGG 1 cut(s) 1700
BcoDI GTCTC 2 cut(s) 133, 1599
BfaI CTAG 9 cut(s) 245, 1383, 1482, 1553, 1617, 1662, 1857, 1985, 2460
BfmI CTRYAG 5 cut(s) 150, 219, 666, 1788, 2028
BfrI CTTAAG 1 cut(s) 380
BfuAI ACCTGC 1 cut(s) 2270
BglI GCCNNNNNGGC 1 cut(s) 2051
BglII AGATCT 1 cut(s) 2230
BisI GCNGC 7 cut(s) 655, 988, 1320, 1788, 1791, 1794, 2304
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 7 cut(s) 656, 989, 1321, 1789, 1792, 1795, 2305
BmcAI AGTACT 2 cut(s) 2413, 2422
Bme1390I CCNGG 3 cut(s) 471, 1285, 1700
Bme18I GGWCC 3 cut(s) 104, 506, 1702
BmgBI CACGTC 1 cut(s) 899
BmgT120I GGNCC 4 cut(s) 104, 506, 604, 1702
BmiI GGNNCC 8 cut(s) 105, 270, 605, 765, 1456, 1703, 2204, 2446
BmrFI CCNGG 3 cut(s) 471, 1285, 1700
BmrI ACTGGG 2 cut(s) 110, 1109
BmsI GCATC 4 cut(s) 1558, 1601, 1613, 1648
BmuI ACTGGG 2 cut(s) 110, 1109
BoxI GACNNNNGTC 1 cut(s) 246
BpiI GAAGAC 2 cut(s) 1494, 2373
BplI GAGNNNNNCTC 4 cut(s) 2004, 2036, 2208, 2240
BpmI CTGGAG 5 cut(s) 115, 453, 1131, 1344, 2342
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 1700
Bsa29I ATCGAT 1 cut(s) 1259
BsaAI YACGTR 1 cut(s) 1507
BsaBI GATNNNNATC 3 cut(s) 1348, 1399, 1672
BsaHI GRCGYC 2 cut(s) 1557, 2075
BsaJI CCNNGG 7 cut(s) 200, 244, 538, 1071, 1705, 2206, 2298
BsaWI WCCGGW 1 cut(s) 2170
Bsc4I CCNNNNNNNGG 5 cut(s) 983, 1147, 1199, 2154, 2320
Bse1I ACTGG 7 cut(s) 105, 607, 939, 1104, 1563, 2325, 2345
Bse3DI GCAATG 3 cut(s) 231, 419, 828
Bse8I GATNNNNATC 3 cut(s) 1348, 1399, 1672
BseBI CCWGG 2 cut(s) 471, 1285
BseCI ATCGAT 1 cut(s) 1259
BseDI CCNNGG 7 cut(s) 200, 244, 538, 1071, 1705, 2206, 2298
BseGI GGATG 6 cut(s) 587, 790, 832, 2002, 2132, 2225
BseJI GATNNNNATC 3 cut(s) 1348, 1399, 1672
BseLI CCNNNNNNNGG 5 cut(s) 983, 1147, 1199, 2154, 2320
BseMI GCAATG 3 cut(s) 231, 419, 828
BseMII CTCAG 3 cut(s) 1145, 1233, 2406
BseNI ACTGG 7 cut(s) 105, 607, 939, 1104, 1563, 2325, 2345
BseRI GAGGAG 2 cut(s) 16, 1618
BseSI GKGCMC 1 cut(s) 1752
BseXI GCAGC 6 cut(s) 666, 1331, 1774, 1802, 1805, 2315
BseYI CCCAGC 2 cut(s) 1413, 2262
BsgI GTGCAG 2 cut(s) 426, 1338
Bsh1236I CGCG 1 cut(s) 1778
BshFI GGCC 4 cut(s) 606, 987, 1961, 2066
BshVI ATCGAT 1 cut(s) 1259
BsiHKAI GWGCWC 3 cut(s) 159, 1973, 2330
BsiSI CCGG 3 cut(s) 1699, 2171, 2314
BslFI GGGAC 2 cut(s) 1430, 1715
BslI CCNNNNNNNGG 5 cut(s) 983, 1147, 1199, 2154, 2320
BsmAI GTCTC 2 cut(s) 133, 1599
BsmFI GGGAC 2 cut(s) 1430, 1715
BsnI GGCC 4 cut(s) 606, 987, 1961, 2066
Bsp1286I GDGCHC 4 cut(s) 159, 1752, 1973, 2330
Bsp1407I TGTACA 3 cut(s) 584, 693, 1121
Bsp143I GATC 2 cut(s) 542, 2230
Bsp19I CCATGG 4 cut(s) 200, 1071, 1705, 2298
BspACI CCGC 1 cut(s) 988
BspANI GGCC 4 cut(s) 606, 987, 1961, 2066
BspCNI CTCAG 3 cut(s) 1146, 1232, 2407
BspDI ATCGAT 1 cut(s) 1259
BspFNI CGCG 1 cut(s) 1778
BspLI GGNNCC 8 cut(s) 105, 270, 605, 765, 1456, 1703, 2204, 2446
BspMAI CTGCAG 2 cut(s) 670, 1792
BspMI ACCTGC 1 cut(s) 2270
BspPI GGATC 1 cut(s) 550
BspQI GCTCTTC 2 cut(s) 402, 2121
BspTI CTTAAG 1 cut(s) 380
BsrDI GCAATG 3 cut(s) 231, 419, 828
BsrGI TGTACA 3 cut(s) 584, 693, 1121
BsrI ACTGG 7 cut(s) 105, 607, 939, 1104, 1563, 2325, 2345
BssECI CCNNGG 7 cut(s) 200, 244, 538, 1071, 1705, 2206, 2298
BssMI GATC 2 cut(s) 542, 2230
BssNI GRCGYC 2 cut(s) 1557, 2075
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 6 cut(s) 200, 244, 1071, 1705, 2206, 2298
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 2 cut(s) 471, 1285
Bst6I CTCTTC 3 cut(s) 214, 402, 2121
BstACI GRCGYC 2 cut(s) 1557, 2075
BstAFI CTTAAG 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 1802
BstAUI TGTACA 3 cut(s) 584, 693, 1121
BstBAI YACGTR 1 cut(s) 1507
BstDEI CTNAG 6 cut(s) 861, 1154, 1219, 1654, 2061, 2415
BstDSI CCRYGG 5 cut(s) 200, 538, 1071, 1705, 2298
BstF5I GGATG 6 cut(s) 587, 790, 832, 2002, 2132, 2225
BstFNI CGCG 1 cut(s) 1778
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 2 cut(s) 545, 2233
BstMAI GTCTC 2 cut(s) 133, 1599
BstMBI GATC 2 cut(s) 542, 2230
BstMWI GCNNNNNNNGC 8 cut(s) 392, 612, 1756, 1784, 1793, 1802, 2051, 2303
BstNI CCWGG 2 cut(s) 471, 1285
BstNSI RCATGY 1 cut(s) 2366
BstPAI GACNNNNGTC 1 cut(s) 246
BstSCI CCNGG 3 cut(s) 469, 1283, 1698
BstSFI CTRYAG 5 cut(s) 150, 219, 666, 1788, 2028
BstSLI GKGCMC 1 cut(s) 1752
BstUI CGCG 1 cut(s) 1778
BstV1I GCAGC 6 cut(s) 666, 1331, 1774, 1802, 1805, 2315
BstV2I GAAGAC 2 cut(s) 1494, 2373
BstX2I RGATCY 2 cut(s) 542, 2230
BstXI CCANNNNNNTGG 1 cut(s) 477
BstYI RGATCY 2 cut(s) 542, 2230
Bsu15I ATCGAT 1 cut(s) 1259
BsuRI GGCC 4 cut(s) 606, 987, 1961, 2066
BsuTUI ATCGAT 1 cut(s) 1259
BtgI CCRYGG 5 cut(s) 200, 538, 1071, 1705, 2298
BtrI CACGTC 1 cut(s) 899
BtsCI GGATG 6 cut(s) 587, 790, 832, 2002, 2132, 2225
BtsI GCAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 2270
CaiI CAGNNNCTG 1 cut(s) 1686
CfoI GCGC 1 cut(s) 908
Cfr13I GGNCC 4 cut(s) 104, 506, 604, 1702
ClaI ATCGAT 1 cut(s) 1259
CseI GACGC 3 cut(s) 526, 1565, 1767
Csp6I GTAC 9 cut(s) 585, 694, 702, 1122, 1369, 1981, 2273, 2412, 2421
CspCI CAANNNNNGTGG 2 cut(s) 1549, 1584
CviQI GTAC 9 cut(s) 585, 694, 702, 1122, 1369, 1981, 2273, 2412, 2421
DdeI CTNAG 6 cut(s) 861, 1154, 1219, 1654, 2061, 2415
DpnI GATC 2 cut(s) 544, 2232
DpnII GATC 2 cut(s) 542, 2230
EaeI YGGCCR 2 cut(s) 985, 1959
Eam1104I CTCTTC 3 cut(s) 214, 402, 2121
EarI CTCTTC 3 cut(s) 214, 402, 2121
Eco130I CCWWGG 6 cut(s) 200, 244, 1071, 1705, 2206, 2298
Eco47I GGWCC 3 cut(s) 104, 506, 1702
Eco57I CTGAAG 7 cut(s) 121, 283, 383, 541, 663, 900, 1127
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 469, 1283
EcoT14I CCWWGG 6 cut(s) 200, 244, 1071, 1705, 2206, 2298
ErhI CCWWGG 6 cut(s) 200, 244, 1071, 1705, 2206, 2298
FaqI GGGAC 2 cut(s) 1430, 1715
FauNDI CATATG 1 cut(s) 1754
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 7 cut(s) 655, 988, 1320, 1788, 1791, 1794, 2304
FokI GGATG 6 cut(s) 594, 797, 839, 2009, 2139, 2232
Fsp4HI GCNGC 7 cut(s) 655, 988, 1320, 1788, 1791, 1794, 2304
FspBI CTAG 9 cut(s) 245, 1383, 1482, 1553, 1617, 1662, 1857, 1985, 2460
GlaI GCGC 1 cut(s) 907
GluI GCNGC 7 cut(s) 655, 988, 1320, 1788, 1791, 1794, 2304
GsaI CCCAGC 2 cut(s) 1417, 2266
GsuI CTGGAG 5 cut(s) 115, 453, 1131, 1344, 2342
HaeIII GGCC 4 cut(s) 606, 987, 1961, 2066
HapII CCGG 3 cut(s) 1699, 2171, 2314
HgaI GACGC 3 cut(s) 526, 1565, 1767
HhaI GCGC 1 cut(s) 908
Hin1I GRCGYC 2 cut(s) 1557, 2075
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 3 cut(s) 450, 1015, 2457
HindII GTYRAC 3 cut(s) 450, 1015, 2457
HpaII CCGG 3 cut(s) 1699, 2171, 2314
HphI GGTGA 2 cut(s) 1855, 2359
Hpy166II GTNNAC 8 cut(s) 241, 256, 377, 450, 1015, 1097, 1981, 2457
Hpy188III TCNNGA 5 cut(s) 160, 1235, 1301, 1553, 1913
Hpy8I GTNNAC 8 cut(s) 241, 256, 377, 450, 1015, 1097, 1981, 2457
Hpy99I CGWCG 1 cut(s) 98
HpyCH4IV ACGT 7 cut(s) 237, 845, 898, 1506, 1515, 2075, 2378
HpyF10VI GCNNNNNNNGC 8 cut(s) 392, 612, 1756, 1784, 1793, 1802, 2051, 2303
HpyF3I CTNAG 6 cut(s) 861, 1154, 1219, 1654, 2061, 2415
HpySE526I ACGT 7 cut(s) 237, 845, 898, 1506, 1515, 2075, 2378
Hsp92I GRCGYC 2 cut(s) 1557, 2075
HspAI GCGC 1 cut(s) 906
Kzo9I GATC 2 cut(s) 542, 2230
LguI GCTCTTC 2 cut(s) 402, 2121
LmnI GCTCC 5 cut(s) 29, 1112, 1460, 1631, 2444
Lsp1109I GCAGC 6 cut(s) 666, 1331, 1774, 1802, 1805, 2315
LweI GCATC 4 cut(s) 1558, 1601, 1613, 1648
MaeI CTAG 9 cut(s) 245, 1383, 1482, 1553, 1617, 1662, 1857, 1985, 2460
MaeII ACGT 7 cut(s) 237, 845, 898, 1506, 1515, 2075, 2378
MaeIII GTNAC 5 cut(s) 899, 1246, 1694, 2056, 2365
MalI GATC 2 cut(s) 544, 2232
MboI GATC 2 cut(s) 542, 2230
MfeI CAATTG 2 cut(s) 1242, 2100
MflI RGATCY 2 cut(s) 542, 2230
MhlI GDGCHC 4 cut(s) 159, 1752, 1973, 2330
MlyI GAGTC 2 cut(s) 1187, 1211
MmeI TCCRAC 1 cut(s) 2155
MroXI GAANNNNTTC 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 1887, 1976
MspA1I CMGCKG 4 cut(s) 990, 1322, 1413, 2027
MspCI CTTAAG 1 cut(s) 380
MspI CCGG 3 cut(s) 1699, 2171, 2314
MspR9I CCNGG 3 cut(s) 471, 1285, 1700
MunI CAATTG 2 cut(s) 1242, 2100
MvaI CCWGG 2 cut(s) 471, 1285
MvnI CGCG 1 cut(s) 1778
MwoI GCNNNNNNNGC 8 cut(s) 392, 612, 1756, 1784, 1793, 1802, 2051, 2303
NciI CCSGG 1 cut(s) 1700
NcoI CCATGG 4 cut(s) 200, 1071, 1705, 2298
NdeI CATATG 1 cut(s) 1754
NdeII GATC 2 cut(s) 542, 2230
NlaIV GGNNCC 8 cut(s) 105, 270, 605, 765, 1456, 1703, 2204, 2446
NmuCI GTSAC 3 cut(s) 899, 1694, 2365
NspI RCATGY 1 cut(s) 2366
OliI CACNNNNGTG 1 cut(s) 1976
PciSI GCTCTTC 2 cut(s) 402, 2121
PcsI WCGNNNNNNNCGW 1 cut(s) 1512
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 8 cut(s) 16, 40, 347, 920, 1076, 1238, 2178, 2468
PflMI CCANNNNNTGG 1 cut(s) 983
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 7 cut(s) 656, 989, 1321, 1789, 1792, 1795, 2305
PleI GAGTC 2 cut(s) 1186, 1211
PpsI GAGTC 2 cut(s) 1186, 1211
Ppu21I YACGTR 1 cut(s) 1507
PpuMI RGGWCCY 1 cut(s) 104
PshAI GACNNNNGTC 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 879
Psp1406I AACGTT 1 cut(s) 845
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 469, 1283
PspFI CCCAGC 2 cut(s) 1413, 2262
PspGI CCWGG 2 cut(s) 469, 1283
PspN4I GGNNCC 8 cut(s) 105, 270, 605, 765, 1456, 1703, 2204, 2446
PspPI GGNCC 4 cut(s) 104, 506, 604, 1702
PspPPI RGGWCCY 1 cut(s) 104
PstI CTGCAG 2 cut(s) 670, 1792
PstNI CAGNNNCTG 1 cut(s) 1686
PsuI RGATCY 2 cut(s) 542, 2230
PvuII CAGCTG 3 cut(s) 1322, 1413, 2027
RsaI GTAC 9 cut(s) 586, 695, 703, 1123, 1370, 1982, 2274, 2413, 2422
RsaNI GTAC 9 cut(s) 585, 694, 702, 1122, 1369, 1981, 2273, 2412, 2421
RseI CAYNNNNRTG 2 cut(s) 1887, 1976
SapI GCTCTTC 2 cut(s) 402, 2121
SatI GCNGC 7 cut(s) 655, 988, 1320, 1788, 1791, 1794, 2304
Sau3AI GATC 2 cut(s) 542, 2230
Sau96I GGNCC 4 cut(s) 104, 506, 604, 1702
ScaI AGTACT 2 cut(s) 2413, 2422
SchI GAGTC 2 cut(s) 1187, 1211
ScrFI CCNGG 3 cut(s) 471, 1285, 1700
SduI GDGCHC 4 cut(s) 159, 1752, 1973, 2330
SfaNI GCATC 4 cut(s) 1558, 1601, 1613, 1648
SfcI CTRYAG 5 cut(s) 150, 219, 666, 1788, 2028
SinI GGWCC 3 cut(s) 104, 506, 1702
SmiMI CAYNNNNRTG 2 cut(s) 1887, 1976
SmlI CTYRAG 2 cut(s) 158, 380
SmoI CTYRAG 2 cut(s) 158, 380
SsiI CCGC 1 cut(s) 988
SspI AATATT 1 cut(s) 2083
SspMI CTAG 9 cut(s) 245, 1383, 1482, 1553, 1617, 1662, 1857, 1985, 2460
StyD4I CCNGG 3 cut(s) 469, 1283, 1698
StyI CCWWGG 6 cut(s) 200, 244, 1071, 1705, 2206, 2298
TaiI ACGT 7 cut(s) 240, 848, 901, 1509, 1518, 2078, 2381
TaqI TCGA 6 cut(s) 14, 233, 1259, 1302, 1342, 1819
TaqII GACCGA 1 cut(s) 35
TatI WGTACW 7 cut(s) 584, 693, 701, 1121, 2272, 2411, 2420
TauI GCSGC 1 cut(s) 990
TfiI GAWTC 8 cut(s) 16, 40, 347, 920, 1076, 1238, 2178, 2468
TseFI GTSAC 3 cut(s) 899, 1694, 2365
TseI GCWGC 6 cut(s) 654, 1319, 1787, 1790, 1793, 2303
Tsp45I GTSAC 3 cut(s) 899, 1694, 2365
TspDTI ATGAA 8 cut(s) 53, 242, 855, 1910, 1924, 1945, 2013, 2460
Van91I CCANNNNNTGG 1 cut(s) 983
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 104, 506, 1702
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 4 cut(s) 10, 433, 673, 1875
XbaI TCTAGA 1 cut(s) 1552
XceI RCATGY 1 cut(s) 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 1281
XmaJI CCTAGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 9 cut(s) 245, 1383, 1482, 1553, 1617, 1662, 1857, 1985, 2460
ZraI GACGTC 1 cut(s) 2076
ZrmI AGTACT 2 cut(s) 2413, 2422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.