Rroxscaffold_4G00299350

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003810
Physical Location & Seq
Forward (+)
19270467 .. 19278370
7904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299350.1

Sequence Viewer

Length: 2289 bp
ATGTCTATAGCAATGAACTCGAACGTAGATCTAGGTCATGTAAACATAGAAAAGGTTCCTAAAATTACAATGCCGTTGATTTGCTTCAGAGATGGTAGCCCTCCACTGCCTGATGTCAATACCTCTCTAACAGATTCAGGCCATCAAGTTTTAGTTCCCTTTTACGATGGTTCGCTTAAGGCAATACAAACTCTTCAGAGTGCAGAGCAATGCTATCCTATGCAAAAGAATTTCACAGTGAAGTTGACAAATGAGCAGATTAACCGTGGATCTCAATATACCTTCAGTGCCAACATCACAGTTGAAGGATGTACAACTGAAACCATGTGGGGCCAGTATTGCAACCAGACAATTTATCCTCTTTCTTGTTCTTTATCTGGCAGCTACAATCCTGCAGAAAATTCTTCAGAGGCTAATTTGTACAACCGTACAATGGGTTATGGTATTTCTTGCAAAAATGATTTTCAAACTTCTTGTCAAGGAGATGGGGAACCAAAGTTTTACTCTTTGGATGTGGTGGGAATATCACAGGAGTTAAACATTGTGGCAATGGATGTTTGGTTAAATGAAACATTATCAAATAACACTAAGAATGGTAGTGATATTAATTTAATGTGTTTTGCACGTCATGGCGCAATACCTTCAGAATCTCTGCATGATTATTCCAGTAATATAAACAATAATCCTTTGGTCATTAACTTTCCAAAGGTTGGCCGCTGGTATATTACTGTTTTACCAGTCAACCTCTCAAAAGTGCTTGGAGGGAGTTTAGATACTTCCATAAAAGTTTGCTACTCCATGGAATCAAAACTGCTTGAATGCCCACTGGGGAAGGCTGGAGCAAATTGTACATGGGAAACATACACCCTTCAGACGGTTCTGAGGAAAGGTTCGGGCTTCTTTGAGTCCTACTATTTACCTGATAGTGGAAATGTATCTTCTGATTCAGCCAATTTTCCTCTTGATTTGCTTGTAACCAACACATCGATTGGCGAAAAACCAGAAAATACCTGGACTTATTTTGTTCTCGACATTCCTCGTGGTGCAGCTGGAGGAAATATGCACATTCGATTAACATCAGATAAGAAGATTAATTACGAAGTATATACTAGATTTGGTGGATTACCATCTCTTACCAGCTGGGACTACTATTATGCTAACAGGACAAGCAATAGTGTTGGCTCCATGTTTTTCAACTTATATAACTCTAGTGAAGACAAGGTTGATTTTTACGTGTTGTATGTCAGAGAAGGAACTTGGGGTTTTGGACTAAAGCATCTAGATGCCACCAGTATTGCTTCCAAAGTTCAAACTACCATGTCTCTTTCCCTTGAAAGATGCCCTAGAAGATGCTCCTCACATGGAAAATGCGAAAGTGTCTTAGATGCTAGTGGATTAACATCATACAGCTATTGCTCGTGTGACCGAGACCATGGAGGCTTTGATTGTAGTATTGAAATTGTATCTCATCATGTCTGTTCGGTTAACATAGTCCCTGAAGCAGATGTAGTTAGTTTGTTTGATGTTGTAGGATCTAGATGGCCATCTTTGTGTTATAAAAAGAGCTGGCACATATGGCAATCAATTTTTCTTATCGTGTCAAATGCTGCAGCAGCACTTCCTGCCTTCTATTCCCTTCGACAGAAGGCATGGTCAGAATGGGTGATATATACATCTAGTGGAATTGCAAGTGGAATTTATCATGCCTGTGACGTGGGAACGTGGTGTCCTCTGGATTTTAATACTTTACAGTTCATGGACTTCTGGCTGTCTTTCATGGCCGTAGTGAGCACATTTGTGTACCTAGCTACAATGGATGAAGCTCATAAGAGAGCAGTCCTCACAGCTGTAGCAATTTTTACTGCCCTTATGGCAGTTACTAAGGCCACAAGGACGTCCAATATTGTGATTGTAATGTCAATTGGCATTTCGGCTCTTCTTCTTGGATGGCTGATAGAATTAACAACTAAGTATAGGTTCCAAGGTGTTAGAGGATGGCTCAAAGATCTTATTAAGACAGTTTTCAAAAGATTTCGCTGGGGCTTTGTACTTGCAGGTTCTACTGCATTAGCCATGGCAGCAATCAGCTGGACACTGGAGAGCACTGAAACCTACTGGATTTGGCACAGCCTTTGGCATGTCACCATATATACGTCTTCCTTCTTCTTCCTTTGCTCAAAAGCAAGTACCGAAAATACTGTAGATACTGGTATTGTAGATACTGAGAGTCAGAGACCCCCAAATGGAGCCTATGAGTTGACTAGGCAGGATTCATTGCCAAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

762

Amino Acids

84.89

Weight (kDa)

5.87

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 524 - 714 2.4e-39 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1557
AatII GACGTC 1 cut(s) 1898
Acc36I ACCTGC 1 cut(s) 2045
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 1 cut(s) 715
AclWI GGATC 2 cut(s) 277, 1540
AcoI YGGCCR 3 cut(s) 712, 1541, 1779
AcsI RAATTY 3 cut(s) 229, 400, 1695
AcuI CTGAAG 7 cut(s) 70, 179, 268, 390, 627, 854, 1518
AcyI GRCGYC 1 cut(s) 1895
AfaI GTAC 7 cut(s) 313, 422, 430, 850, 1802, 2049, 2188
AfiI CCNNNNNNNGG 5 cut(s) 433, 710, 874, 926, 2243
AflII CTTAAG 1 cut(s) 176
AflIII ACRYGT 1 cut(s) 1233
AgsI TTSAA 8 cut(s) 305, 467, 818, 1195, 1310, 1334, 1457, 2026
AjiI CACGTC 2 cut(s) 626, 1714
AjnI CCWGG 1 cut(s) 1010
AleI CACNNNNGTG 1 cut(s) 1796
AloI GAACNNNNNNTCC 2 cut(s) 1711, 1743
AluBI AGCT 9 cut(s) 384, 1049, 1140, 1410, 1566, 1808, 1823, 1847, 2088
AluI AGCT 9 cut(s) 384, 1049, 1140, 1410, 1566, 1808, 1823, 1847, 2088
Alw21I GWGCWC 2 cut(s) 1793, 2105
Alw26I GTCTC 3 cut(s) 1326, 1422, 2227
AlwI GGATC 2 cut(s) 277, 1540
AoxI GGCC 6 cut(s) 139, 331, 712, 1541, 1779, 1884
ApeKI GCWGC 6 cut(s) 381, 1046, 1607, 1610, 1613, 2078
ApoI RAATTY 3 cut(s) 229, 400, 1695
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
AseI ATTAAT 2 cut(s) 606, 1092
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 1675, 2134
BalI TGGCCA 1 cut(s) 1543
BarI GAAGNNNNNNTAC 2 cut(s) 1079, 1111
BauI CACGAG 2 cut(s) 1038, 1417
BbsI GAAGAC 2 cut(s) 1221, 2148
Bbv12I GWGCWC 2 cut(s) 1793, 2105
BbvI GCAGC 6 cut(s) 393, 1058, 1594, 1622, 1625, 2090
BccI CCATC 9 cut(s) 86, 150, 161, 479, 1135, 1533, 1552, 1941, 1989
BceAI ACGGC 2 cut(s) 58, 1766
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 1012
BcoDI GTCTC 3 cut(s) 1326, 1422, 2227
BfmI CTRYAG 5 cut(s) 6, 393, 1608, 1848, 2199
BfrI CTTAAG 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 2045
BglI GCCNNNNNGGC 1 cut(s) 1871
BglII AGATCT 2 cut(s) 28, 2005
BisI GCNGC 7 cut(s) 382, 715, 1047, 1608, 1611, 1614, 2079
BlsI GCNGC 7 cut(s) 383, 716, 1048, 1609, 1612, 1615, 2080
Bme1390I CCNGG 1 cut(s) 1012
BmgBI CACGTC 2 cut(s) 626, 1714
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 6 cut(s) 57, 332, 492, 1183, 1979, 2248
BmrFI CCNGG 1 cut(s) 1012
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 5 cut(s) 1273, 1285, 1328, 1340, 1375
BmuI ACTGGG 1 cut(s) 836
BpiI GAAGAC 2 cut(s) 1221, 2148
BplI GAGNNNNNCTC 4 cut(s) 1824, 1856, 1983, 2015
BpmI CTGGAG 3 cut(s) 858, 1071, 2117
Bsa29I ATCGAT 1 cut(s) 986
BsaAI YACGTR 1 cut(s) 1234
BsaBI GATNNNNATC 4 cut(s) 1075, 1126, 1399, 1543
BsaHI GRCGYC 1 cut(s) 1895
BsaI GGTCTC 2 cut(s) 1422, 2227
BsaJI CCNNGG 5 cut(s) 265, 798, 1432, 1981, 2073
Bsc4I CCNNNNNNNGG 5 cut(s) 433, 710, 874, 926, 2243
Bse1I ACTGG 8 cut(s) 334, 666, 737, 831, 1290, 2100, 2120, 2212
Bse3DI GCAATG 4 cut(s) 18, 215, 555, 2273
Bse8I GATNNNNATC 4 cut(s) 1075, 1126, 1399, 1543
BseBI CCWGG 1 cut(s) 1012
BseCI ATCGAT 1 cut(s) 986
BseDI CCNNGG 5 cut(s) 265, 798, 1432, 1981, 2073
BseGI GGATG 6 cut(s) 314, 517, 559, 1822, 1952, 2000
BseJI GATNNNNATC 4 cut(s) 1075, 1126, 1399, 1543
BseLI CCNNNNNNNGG 5 cut(s) 433, 710, 874, 926, 2243
BseMI GCAATG 4 cut(s) 18, 215, 555, 2273
BseMII CTCAG 2 cut(s) 872, 2214
BseNI ACTGG 8 cut(s) 334, 666, 737, 831, 1290, 2100, 2120, 2212
BseRI GAGGAG 1 cut(s) 1345
BseXI GCAGC 6 cut(s) 393, 1058, 1594, 1622, 1625, 2090
BseYI CCCAGC 2 cut(s) 1140, 2037
BsgI GTGCAG 2 cut(s) 222, 1065
BshFI GGCC 6 cut(s) 141, 333, 714, 1543, 1781, 1886
BshVI ATCGAT 1 cut(s) 986
BsiHKAI GWGCWC 2 cut(s) 1793, 2105
BslFI GGGAC 2 cut(s) 1157, 1478
BslI CCNNNNNNNGG 5 cut(s) 433, 710, 874, 926, 2243
BsmAI GTCTC 3 cut(s) 1326, 1422, 2227
BsmFI GGGAC 2 cut(s) 1157, 1478
BsmI GAATGC 1 cut(s) 824
BsnI GGCC 6 cut(s) 141, 333, 714, 1543, 1781, 1886
Bso31I GGTCTC 2 cut(s) 1422, 2227
Bsp1286I GDGCHC 2 cut(s) 1793, 2105
Bsp1407I TGTACA 3 cut(s) 311, 420, 848
Bsp143I GATC 4 cut(s) 28, 269, 1532, 2005
Bsp19I CCATGG 3 cut(s) 798, 1432, 2073
BspACI CCGC 1 cut(s) 715
BspANI GGCC 6 cut(s) 141, 333, 714, 1543, 1781, 1886
BspCNI CTCAG 2 cut(s) 873, 2215
BspDI ATCGAT 1 cut(s) 986
BspLI GGNNCC 6 cut(s) 57, 332, 492, 1183, 1979, 2248
BspMAI CTGCAG 2 cut(s) 397, 1612
BspMI ACCTGC 1 cut(s) 2045
BspPI GGATC 2 cut(s) 277, 1540
BspQI GCTCTTC 1 cut(s) 1941
BspTI CTTAAG 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 1422, 2227
BsrDI GCAATG 4 cut(s) 18, 215, 555, 2273
BsrGI TGTACA 3 cut(s) 311, 420, 848
BsrI ACTGG 8 cut(s) 334, 666, 737, 831, 1290, 2100, 2120, 2212
BssECI CCNNGG 5 cut(s) 265, 798, 1432, 1981, 2073
BssMI GATC 4 cut(s) 28, 269, 1532, 2005
BssNI GRCGYC 1 cut(s) 1895
BssSI CACGAG 2 cut(s) 1038, 1417
BssT1I CCWWGG 4 cut(s) 798, 1432, 1981, 2073
Bst2BI CACGAG 2 cut(s) 1038, 1417
Bst2UI CCWGG 1 cut(s) 1012
Bst4CI ACNGT 9 cut(s) 238, 266, 301, 428, 730, 877, 1752, 2020, 2200
Bst6I CTCTTC 2 cut(s) 198, 1941
BstACI GRCGYC 1 cut(s) 1895
BstAFI CTTAAG 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 1622
BstAUI TGTACA 3 cut(s) 311, 420, 848
BstBAI YACGTR 1 cut(s) 1234
BstC8I GCNNGC 1 cut(s) 1568
BstDEI CTNAG 6 cut(s) 588, 881, 1381, 1881, 1968, 2223
BstDSI CCRYGG 4 cut(s) 265, 798, 1432, 2073
BstF5I GGATG 6 cut(s) 314, 517, 559, 1822, 1952, 2000
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 4 cut(s) 31, 272, 1535, 2008
BstMAI GTCTC 3 cut(s) 1326, 1422, 2227
BstMBI GATC 4 cut(s) 28, 269, 1532, 2005
BstMWI GCNNNNNNNGC 6 cut(s) 339, 1576, 1613, 1622, 1871, 2078
BstNI CCWGG 1 cut(s) 1012
BstNSI RCATGY 1 cut(s) 2141
BstSCI CCNGG 1 cut(s) 1010
BstSFI CTRYAG 5 cut(s) 6, 393, 1608, 1848, 2199
BstV1I GCAGC 6 cut(s) 393, 1058, 1594, 1622, 1625, 2090
BstV2I GAAGAC 2 cut(s) 1221, 2148
BstX2I RGATCY 4 cut(s) 28, 269, 1532, 2005
BstYI RGATCY 4 cut(s) 28, 269, 1532, 2005
Bsu15I ATCGAT 1 cut(s) 986
BsuRI GGCC 6 cut(s) 141, 333, 714, 1543, 1781, 1886
BsuTUI ATCGAT 1 cut(s) 986
BtgI CCRYGG 4 cut(s) 265, 798, 1432, 2073
BtrI CACGTC 2 cut(s) 626, 1714
BtsCI GGATG 6 cut(s) 314, 517, 559, 1822, 1952, 2000
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 6 cut(s) 104, 243, 292, 824, 2093, 2103
BveI ACCTGC 1 cut(s) 2045
Cac8I GCNNGC 1 cut(s) 1568
CfoI GCGC 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 331
ClaI ATCGAT 1 cut(s) 986
Csp6I GTAC 7 cut(s) 312, 421, 429, 849, 1801, 2048, 2187
CspCI CAANNNNNGTGG 2 cut(s) 1276, 1311
CviQI GTAC 7 cut(s) 312, 421, 429, 849, 1801, 2048, 2187
DdeI CTNAG 6 cut(s) 588, 881, 1381, 1881, 1968, 2223
DpnI GATC 4 cut(s) 30, 271, 1534, 2007
DpnII GATC 4 cut(s) 28, 269, 1532, 2005
EaeI YGGCCR 3 cut(s) 712, 1541, 1779
Eam1104I CTCTTC 2 cut(s) 198, 1941
EarI CTCTTC 2 cut(s) 198, 1941
Eco130I CCWWGG 4 cut(s) 798, 1432, 1981, 2073
Eco31I GGTCTC 2 cut(s) 1422, 2227
Eco57I CTGAAG 7 cut(s) 70, 179, 268, 390, 627, 854, 1518
EcoRII CCWGG 1 cut(s) 1010
EcoT14I CCWWGG 4 cut(s) 798, 1432, 1981, 2073
ErhI CCWWGG 4 cut(s) 798, 1432, 1981, 2073
FaqI GGGAC 2 cut(s) 1157, 1478
FauNDI CATATG 1 cut(s) 1574
Fnu4HI GCNGC 7 cut(s) 382, 715, 1047, 1608, 1611, 1614, 2079
FokI GGATG 6 cut(s) 321, 524, 566, 1829, 1959, 2007
Fsp4HI GCNGC 7 cut(s) 382, 715, 1047, 1608, 1611, 1614, 2079
GlaI GCGC 1 cut(s) 634
GluI GCNGC 7 cut(s) 382, 715, 1047, 1608, 1611, 1614, 2079
GsaI CCCAGC 2 cut(s) 1144, 2041
GsuI CTGGAG 3 cut(s) 858, 1071, 2117
HaeIII GGCC 6 cut(s) 141, 333, 714, 1543, 1781, 1886
HhaI GCGC 1 cut(s) 635
Hin1I GRCGYC 1 cut(s) 1895
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 4 cut(s) 246, 742, 1486, 2259
HindII GTYRAC 4 cut(s) 246, 742, 1486, 2259
HinfI GANTC 7 cut(s) 134, 647, 803, 905, 944, 2227, 2270
HpaI GTTAAC 1 cut(s) 1486
HphI GGTGA 2 cut(s) 1675, 2134
Hpy166II GTNNAC 6 cut(s) 43, 246, 742, 1486, 1801, 2259
Hpy188III TCNNGA 5 cut(s) 962, 1028, 1280, 1536, 1733
Hpy8I GTNNAC 6 cut(s) 43, 246, 742, 1486, 1801, 2259
HpyCH4III ACNGT 9 cut(s) 238, 266, 301, 428, 730, 877, 1752, 2020, 2200
HpyCH4IV ACGT 7 cut(s) 24, 625, 1233, 1713, 1721, 1895, 2153
HpyF10VI GCNNNNNNNGC 6 cut(s) 339, 1576, 1613, 1622, 1871, 2078
HpyF3I CTNAG 6 cut(s) 588, 881, 1381, 1881, 1968, 2223
HpySE526I ACGT 7 cut(s) 24, 625, 1233, 1713, 1721, 1895, 2153
Hsp92I GRCGYC 1 cut(s) 1895
HspAI GCGC 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 1486
Kzo9I GATC 4 cut(s) 28, 269, 1532, 2005
LguI GCTCTTC 1 cut(s) 1941
LmnI GCTCC 4 cut(s) 839, 1187, 1358, 2246
Lsp1109I GCAGC 6 cut(s) 393, 1058, 1594, 1622, 1625, 2090
LweI GCATC 5 cut(s) 1273, 1285, 1328, 1340, 1375
MaeII ACGT 7 cut(s) 24, 625, 1233, 1713, 1721, 1895, 2153
MaeIII GTNAC 5 cut(s) 973, 1421, 1709, 1876, 2140
MalI GATC 4 cut(s) 30, 271, 1534, 2007
MboI GATC 4 cut(s) 28, 269, 1532, 2005
MfeI CAATTG 1 cut(s) 1920
MflI RGATCY 4 cut(s) 28, 269, 1532, 2005
MhlI GDGCHC 2 cut(s) 1793, 2105
MlsI TGGCCA 1 cut(s) 1543
MluNI TGGCCA 1 cut(s) 1543
MlyI GAGTC 2 cut(s) 914, 2236
Mox20I TGGCCA 1 cut(s) 1543
MroXI GAANNNNTTC 1 cut(s) 54
MscI TGGCCA 1 cut(s) 1543
MslI CAYNNNNRTG 4 cut(s) 1281, 1549, 1707, 1796
Msp20I TGGCCA 1 cut(s) 1543
MspA1I CMGCKG 5 cut(s) 717, 1049, 1140, 1847, 2088
MspCI CTTAAG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 1012
MunI CAATTG 1 cut(s) 1920
Mva1269I GAATGC 1 cut(s) 824
MvaI CCWGG 1 cut(s) 1012
MwoI GCNNNNNNNGC 6 cut(s) 339, 1576, 1613, 1622, 1871, 2078
NcoI CCATGG 3 cut(s) 798, 1432, 2073
NdeI CATATG 1 cut(s) 1574
NdeII GATC 4 cut(s) 28, 269, 1532, 2005
NlaIV GGNNCC 6 cut(s) 57, 332, 492, 1183, 1979, 2248
NmuCI GTSAC 3 cut(s) 1421, 1709, 2140
NspI RCATGY 1 cut(s) 2141
OliI CACNNNNGTG 1 cut(s) 1796
PciSI GCTCTTC 1 cut(s) 1941
PctI GAATGC 1 cut(s) 824
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 5 cut(s) 134, 647, 803, 944, 2270
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 7 cut(s) 383, 716, 1048, 1609, 1612, 1615, 2080
PleI GAGTC 2 cut(s) 913, 2235
PpsI GAGTC 2 cut(s) 913, 2235
Ppu21I YACGTR 1 cut(s) 1234
PshBI ATTAAT 2 cut(s) 606, 1092
PsiI TTATAA 1 cut(s) 1557
Psp6I CCWGG 1 cut(s) 1010
PspFI CCCAGC 2 cut(s) 1140, 2037
PspGI CCWGG 1 cut(s) 1010
PspN4I GGNNCC 6 cut(s) 57, 332, 492, 1183, 1979, 2248
PspPI GGNCC 1 cut(s) 331
PstI CTGCAG 2 cut(s) 397, 1612
PsuI RGATCY 4 cut(s) 28, 269, 1532, 2005
PvuII CAGCTG 4 cut(s) 1049, 1140, 1847, 2088
RsaI GTAC 7 cut(s) 313, 422, 430, 850, 1802, 2049, 2188
RsaNI GTAC 7 cut(s) 312, 421, 429, 849, 1801, 2048, 2187
RseI CAYNNNNRTG 4 cut(s) 1281, 1549, 1707, 1796
SapI GCTCTTC 1 cut(s) 1941
SatI GCNGC 7 cut(s) 382, 715, 1047, 1608, 1611, 1614, 2079
Sau3AI GATC 4 cut(s) 28, 269, 1532, 2005
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 2 cut(s) 914, 2236
ScrFI CCNGG 1 cut(s) 1012
SduI GDGCHC 2 cut(s) 1793, 2105
SfaNI GCATC 5 cut(s) 1273, 1285, 1328, 1340, 1375
SfcI CTRYAG 5 cut(s) 6, 393, 1608, 1848, 2199
SmiMI CAYNNNNRTG 4 cut(s) 1281, 1549, 1707, 1796
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SsiI CCGC 1 cut(s) 715
SspI AATATT 1 cut(s) 1903
StyD4I CCNGG 1 cut(s) 1010
StyI CCWWGG 4 cut(s) 798, 1432, 1981, 2073
TaaI ACNGT 9 cut(s) 238, 266, 301, 428, 730, 877, 1752, 2020, 2200
TaiI ACGT 7 cut(s) 27, 628, 1236, 1716, 1724, 1898, 2156
TaqI TCGA 5 cut(s) 20, 986, 1029, 1069, 1639
TaqII GACCGA 1 cut(s) 1440
TatI WGTACW 4 cut(s) 311, 420, 848, 2047
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 5 cut(s) 134, 647, 803, 944, 2270
TscAI CASTG 6 cut(s) 111, 243, 292, 831, 2100, 2110
TseFI GTSAC 3 cut(s) 1421, 1709, 2140
TseI GCWGC 6 cut(s) 381, 1046, 1607, 1610, 1613, 2078
Tsp45I GTSAC 3 cut(s) 1421, 1709, 2140
TspDTI ATGAA 6 cut(s) 29, 582, 1744, 1765, 1833, 2262
TspRI CASTG 6 cut(s) 111, 243, 292, 831, 2100, 2110
Van91I CCANNNNNTGG 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 176
VspI ATTAAT 2 cut(s) 606, 1092
XapI RAATTY 3 cut(s) 229, 400, 1695
XbaI TCTAGA 2 cut(s) 1279, 1535
XceI RCATGY 1 cut(s) 2141
XcmI CCANNNNNNNNNTGG 1 cut(s) 1008
XmnI GAANNNNTTC 1 cut(s) 54
ZraI GACGTC 1 cut(s) 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.