AT2G48060

Piezo-type mechanosensitive ion channel homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006607
Physical Location & Seq
Reverse (-)
19651108 .. 19662723
11616 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G48060.3

Sequence Viewer

Length: 7389 bp
ATGGCGAGTTTTTTGGTGGGCTTCTTGTTGCCTTCGCTTCTTCTGGCAGCTGCACTTATTAACTGGAGTGTCATATCCTTTCTTGATTTGATAGCTTTCCTCCTCGTTCACTATATTGCACCTGAAATTGGATACCGTTTTCAGAGGAGGCATTGGCTATTATGGCCCATTTTCATCTTTTCCTTTGCAGTATTTCTTGCTCAAGTGGTCTACCTTGTCATTTGGGCTGCTCTAGGTCAAGACTGGGATACACCAGATACTGGCTGGATGAGAGTTATTGGCTTTATGATATTAAAGTCTTGGAGAAACCCCACCGTTATGTATTTCCTGGCTTTACAACTACTAACATCATTGGTTGCTTTAGCTGACATATACAGTAGTAGATTCGGCTTCGCTAGATGGCGGGACACATGGTGGTCTCACTTCTCAGGAATTTTCGAACACCTAGGAAGTCATCTTAGGGTTGCCTCCTGCTTGTTGTTACCAGCAGTTCAATTGGCAGTGGGAATCTGCAATCCATCATGGGTTTCTTTGCCATTTTTTATTGGAAGCTGTGCCGGTCTTGTGGACTGGTCCTTGACAAGCAATGTTTCAGGACTTTTCAGGTGGTGGAGAGTTCTTTATATCTACGCAGGATTCAACATTGTCCTGCTTTACCTCTATCAACTACCAATAAACTTTTCCGATATGATTCGTTGGATAGCAAGCTTTATAGGTCTATTCAGAATCTCCCTGGAAACTGAAGGCCCTGATATTTGTTCTGGTCTTTTCCTTGTGTTGTTCTACATCATGCTATCCTATGTAAGGTCAGACCTCGAGGATATGGACTTTATCATGTCCACGAGTGAAAATAACTTGGCAGAGCGGCTTCTTCCTCCAAAATATTCATTTTTTATTCGAGAATCAAGGGCCGGTGTCAGGCACACCAATGTTTTACTGAGGGGAGCTGTGTTTAAGACTTTCAGTATTAATTTCTTCACATATGGTTTTCCGGTCTCTCTATTTGCTCTTTCGTTCTGGAGTTTTCACTTTGCGAGCTTGTGTGCTTTTGGCCTCCTTGCATATGTTGGTTACATTATTTATGCCTTCCCGTCATTGTTCCAGTTGCACAGATTAAATGGCCTTCTTCTCGTATTTATACTCTTATGGGCTGTCAGTACGTATATATTCAATGTGGCCTTTTCTTTTCTGAACACTAAGGTTGGAAAGTTTGGTTTGGGGATGTTGGTCGCGTTGGGTAATCTTGTAAACAATTCTGTTTTTCTGTACCTGTCTGAAGAGAGCTCCAGATCTTCCAATGAGAGATCTTACGTTGAAGCTGATGAAGAAACAAAGGTGTTGGTCGTCGCGACCATTGCTTGGGGATTGCGGAAATGCTCACGGGCTATTATGCTTGCACTGATTTTTCTCATTGCTATGAAACCTGGGTTTTTCCATGCCGTATATGTGATATTCTTCCTTATGTATCTGTTGAGCCACAACATCAACAGAAAGATACGCAAGTCCTTGATTCTACTATGTGAAGTTCATTTTGCACTTCTCTACATTCTTGAGATTGACCTGGTGTCGAACTCCTTAAAGCAGGAAGGCTCTGCGAGCAGAGAAGTTCTATTTCAGTTAGGTCTCCTTAGGTCTGAAAGCTCTTGGGACTTCTTGGAGATAGCCTTGCTAGCTTGCTTCTGTGCTATCCATAATCATGGTTTTGAGGTGCTATTTTCCTTTTCAGCAATTGTACGCCATACACCAAGCCCTCCAATTGGATTTAGCATATTGAAAGCTGGTCTCAACAAATCAGTCCTCTTGTCTGTCTATTCGTCTCCATCTTCAAGTTATAGCCAGGATAATACCACTTACGAGAGACATATTGCTTCATTTCTGAGTGCAATTGGGCAAAAGTTTCTGTCTATGTACCGATCGTGTGGAACATACATTGCTTTCATCACTATTCTCATAAGTGTATACCTGGTCAAACCCAATTATGTATCATTTGGATATATTTTCCTTCTCTTGCTCTGGATTACTGGAAGACAACTGTTTGAAGAAACTAAGAGACGCTTGTGGTTCCCTTTGAAAGCATATGCAGTTTTGGTGTTTATGTTTATCTACTGCTTAAGTAGCTTTGTCAGCTTGCAGCTTTGGTTATCTGGATTTATTGATCTCTACTTTTATTTGGGTTACAACTCTAAAGCCCCATTGCTGGATAATGTCTGGGAATCTCTTGCTGTGTTGATCGTGATGCAACTTTACAGCTATGAAAGGAGGCAGAGTGGACACTACATTCCAGGTCAATCTAGTTTGCTTCATCCTGGGGTTTTTGGATTTTTTGAGAGATTTTTGGCATGGCATGGTCAGAAGATCTTGTTCGCGGCATTGTTTTATGCATCATTGTCTCCGATTAGTGTGTTTGGATTTGTATATCTCCTTGGCCTTGTCATCTGCACAACCTTCCCAAAGTCTTCTTCAATACCATCCAAATCATTCTTGATTTATACTGGATTTCTGGTGTCTGCGGAGTATCTTTTCCAATTGTGGGGGATGCAAGCTCAGATGTTCCCTGGGCAAAAATATGCTGAGTTGTCTTTCTACTTGGGGCTTCGAGTATATGAACCTGGATTTTGGGGTATAGAATCAGGTCTACGAGGAAAAGTGCTGGTTGTTGCAGCCTGTACTCTGCAGTACAATGTATTTCGTTGGCTAGAAAGAACATCTGGTTTAACTGTTATCAAAGGAAAATATGAAGAGCCGTGTCCACTTTTTGTCTCTGCAGAAGACACTACTGCAAGTGTTTCCAGTTCTAATGGTGAAAATCCATCTTCGACTGATCATGCTTCTATATCAATGAAACAAGGCGAGGCAACGAGTAACTCATGGCCTTTCTTCTCTCCCCGTGGTAACCAGGGAGCTGGTTTTTTGCACCCCAAGACTGGAGGCTCTGAAAGTGGCAGTAGTAGGAAATTTTCATTTGGTCACTTCTGGGGAAGTATCAAAGAGAGTCACAGGTGGAACAGGAGGAGAATTCTTGCATTGAAGAAGGAGAGGTTCGAAACACAGAAGAATCTGTTGAAAATTTACTTGAAGTTTTGGATAGAGAATATGTTTAATCTCTATGGCCTTGAGATAAACATGATTGCGCTGCTTCTTGCGAGTTTTGCTTTGCTGAATGCCATCTCCATGGTATATATTGCACTGCTTGCTGCATGTGTCCTCTTGAGAAGACGTGTAATTCAGAAACTATGGCCTGTTGTTGTTTTTCTGTTTGCATCAATTCTCGCAATTGAGTATGTTGCCACATGGAATAGTTTTTTGCCTTCAGATCAGGCTCCAAGTGAAACTAGTGTGCATTGCCATGACTGCTGGAGTATCGCAGCTCTCTACTTTAAATTTTGCCGGGAGTGTTGGCTTGGAGTGAGAGTGGACGACCCCCGGACTCTTATTAGCTATTTTGTGGTATTCATGCTTGCCTGTTTCAAACTTCGGGCTGATCACATATCCAGTTTCTCTGAGTCATCGACATATCATCAGATGAAGTCTCAAAGAAAGAATTCATTTGTCTGGAGAGATCTCTCCTTCGAGACAAAAAGCATGTGGACTGTGCTTGATTACCTGAGGCTATATTGTTATGTCCATCTGCTGGATGTTGTGCTTATTCTGATTCTAATCACAGGAACTCTTGAGTATGACATTCTACACCTGGGTTATCTTGCATTCGCTCTTGTTTTTGCCCGAATGAGACTTGAAATACTGAAGAAGAAAAACAAAATATTCAGGTTCTTGCGGGTGTACAATTTTGTTCTCATCATCTTTTCTCTCGCATATCAGTCTCCATTTGTTGGAAACTTCAATGATGGAAAATGTGAAACGGTTGATTATATATACGAGGTGATTGGATTTTACAAGTATGACTATGGGTTTCGAATCACTGCAAGATCTGCTCTCGTTGAGATCATCATATTTATGCTAGTATCTCTTCAGTCCTACATGTTTTCCTCCCAGGAGTTTGATTATGTGTCGCGGTATCTTGAAGCAGAGCAAATCGGTGCTATTGTGCGGGAGCAAGAGAAGAAAGCAGCACGGAAGACCGAGCAACTGCAACAAATTCGTGAGGCTGAGGAAAAAAAAAGGCAGCGAAATTTGCAGGTGGAAAAGATGAAATCTGAAATGCTGAACCTACGTGTACAGCTTCACAGAATGAATTCTGATTCTAATTTTGGAGTTGCTTCTCCGCGCACTGAAGGTCTAAGAAGGAGGAAGAGTCCGTATCTAATTCCAGATAGTGGTGCAGCCAGTCCTGAGATTGACGGAGTGGTCCACAGGAAAGAAGAGCAGCCTATTGACGAGGATTCACAGTATCCTTTTGAAGCGCATGAGTTTCCAGTGAGTACCACTCCAGAAGCACTAGATTCTCCAGAGTATTCATTTGGAGCATCTCCTTGTGAGATAACTGAAGTTCAACAGGATCTTGATGTGATGTCTATGGAACGTGAAAGAAAGCAAAAGTCTGAGGGAAAGGAGAATCCATTAATTTCTGCGGTGCAACTCATCGGTGATGGTGTTTCCCAGGTACAGTTTATTGGAAATCAGGCAGTAAATAACCTTGTGAATTTCTTAAACATCTCGCCGGAAAATTCAGATACAAATGAGCAGTCTTCTGTTGATGATGAGGTGTATGATGAAATGGAAAGTCAGAAAAGAAAACACACACCTTTCGAACGGTCAACATCTCTACAATCTGACAGGAGTAGTGATGGCACTAGTTTTCAGATAGGAAGGATCTTTCGTCATATATGGTCTAGGATGCAGTCCAATAACGATATTGTTTGCTATTGCTGCTTCATTATTGCGTTTCTCTGGAATTTCAGTCTTCTTTCCATGGTCTATCTAGCAGCACTGTTCCTATACGCCCTCTGCGTTCACACTGGCCCTACTCACATCTTCTGGGTCATCATGCTAATGTACACAGAAATTTATATCCTGCTCCAGTATTTATACCAGATTATAATCCAGCACTGTGGGTTAAGCATTGATGCACCTCTTCTTCATGAATTGGGATTTCCAACGCAAAGAATCAAATCATCATTTGTTGTCAGCTCGTTGCCTCTGTTCCTTATTTACATATTTACTCTCATACAGAGTTCTATAACAGTGAAAGATGGTGATTGGGTTCCTTCTGCTGATTTTACTTCGCGCCGGAATGCCCGTGGTAGCCAGAAGGATCTTACAAGAATTAGATTGAGCCAGAGAATCTTGGATGTGTTTAAGAAGCTAAGAGATAGTGCGAAATTGGTGATCAGAAGCATCTACCGATACTGGATATCTCTGACACGTGGAGCAGAATCCCCTCCGTACTTTGTTCAGGTGACAATGGATGTTCACATGTGGCCTGAAGATGGAATTCAGCCTGAAAGAGTTGAATGTAGAATGAATCAGTTGCTTAGGCTTGTCCATAATGAGAGATGCGAGAAGGGAAACCCTGATCTCTGCCCTTACTCTAGCAGGGTTCACGTACAAAGTATAGAGAGAAGTACAGAAACCCCAAACGAGGCCTTAGTTGTTCTCGAGGTTGAGTATGCTTCTCCCACAAATGGCTGTTCTTCAGCAGAATGGTACAAATCACTTACTCCAGCCTCGGATGTGGCGAAAGAAATTCGTAAAGCTCAACATAGTGGACTTGGTGAAGGAACTGGATTTCCTTACCCCATTCTCTCTGTAATTGGCGGAGGAAAGAGAGATACAGACCTCTACGCCTACATTTTTGGTGCTGACTTGATAGTCTTCTTTCTGGTTGCCATTTTCTACCAATCAGTCATTAAAAATAAAAGCGAGTTTATTGATGTATATCAGCTAGAAGACCAGTTTCCATTCGACTTTGTCATTATTCTTATGGTTATCTTCTTCCTAATTGTTGTTGATCGCGTCATCTATCTTTGCTCATTTGCGACTGGAAAAGTGGTATACTATCTCTTCAGTCTTATTCTCTTCACATATGCAGTGACAGAGTATGCTTGGAGCATATATCCTACCCAGCAACACGCTGCTGGCTTGGCTCTCAGAATCATATTTCTTGCCAAAGCAATGTCACTAGCTCTCCAGGCCATACAAATCCGCTATGGACTACCTCATAAAAGCACCTTGTATCGGCAGTTTTTGACAAGTGAAGTATCACGGATCAATTACTATGGTTACAGACTTTACCGTGCTCTTCCTTTCCTGTATGAACTGAGATGTGTGCTTGACTGGTCTTGCACAGCTACATCACTGACAATGTATGACTGGCTCAAGTTGGAAGATGTAAATGCGAGTTTGTACCTTGTCAAATGCGATACAGTTTTAAATCGAGCAACACATAAACATGGGGAGAAACAAACGAAAATGACTAAATGCTGCAATGGGATATGTCTGTTCTTCATCTTGTTATGTGTTATCTGGGCTCCTATGCTGATGTATAGCAGTGGTAACCCGACAAACATTGCCAATCCGATAAAAGATGCGAGCGTTCAGATTGATTTAAAAACAGTTGGTGGAAAGCTTACTTTGTACCAAACGACCCTATGCGAGAGAATCTCAGGGGATAACATTGATCTCGGGCTAGATCTCGGGTCTCAAAGCTTTTTGCCAACATACAACAAAAATGACATCCAGCTGATATGCTGTCAAGCTGATGCAAGTGTTTTATGGCTTGTCCCTGACACAGTTGTGACCAGATTTATTCAATCCCTTGACTGGGACACAGATATGGACATCACCTTTACTTGGGTTCTTAACAGAGACCGCCCTAAAGGCAAGGAGACTGTGAAATATGAAAGAAGTGTCGACCCTCTGGACCTTCCAAAACGCTCTGATATCCAAATGGTTCTCAATGGGTCGATGGATGGATTTAGAGTGCATAATTTGTACCCAAAGTTCTTCCGTGTTACTGGTTCTGGTGATGTCAGGTCTTTCGAGGATCAGACGGATGAAGTGAGTGCAGACATACTCATTAACCATGCAAATTTCAAGTGGTGGTGGTCATTCCATAATCTTAAAGCGTCTGAAAATATCAGCGCTTGCGAGGGGATGGATGGACCAGTTGCTATCATAATGTCTGAGGAAACACCGCCACAGGGCTTTCTGGGTGACACCCTCAGCAAGTTCAGTATATGGGGACTCTATATCACATTTGTACTAGCGGTGGGGCGTTTCATCAGGCTTCAATGCTCTGACCTGCGTATGAGAATACCTTACGAGAACCTGCCTTCGTGTGACAGATTAATAGCCATATGCGAGGACTTGTACGCGGCTAGAGCAGAGGGTGAGCTTGGAGTAGAAGAAGTTCTATACTGGACGCTTGTGAAGATCTATAGATCCCCGCACATGCTGCTCGAGTATACAAAGCTAGACTATGATGCTTAG

Protein Analysis

2462

Amino Acids

282.27

Weight (kDa)

7.58

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIEZO PF25288 359 - 488 6.2e-62 Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1 PF23188 1512 - 1584 3.7e-15 Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor PF24874 1822 - 2060 7.5e-67 Piezo, THU9 and anchor domain
Piezo_cap PF12166 2088 - 2368 3.9e-78 Piezo non-specific cation channel, cap domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4997
AarI CACCTGC 1 cut(s) 4126
AasI GACNNNNNNGTC 2 cut(s) 4304, 5768
AbsI CCTCGAGG 1 cut(s) 815
Acc36I ACCTGC 3 cut(s) 4126, 7209, 7236
AccB7I CCANNNNNTGG 5 cut(s) 260, 1359, 3631, 3830, 4274
AccBSI CCGCTC 1 cut(s) 865
AccI GTMKAC 6 cut(s) 210, 1959, 2635, 5952, 6810, 7364
AccII CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
AclWI GGATC 6 cut(s) 4464, 4778, 5220, 6173, 6951, 7335
AcvI CACGTG 1 cut(s) 5324
AdeI CACNNNGTG 1 cut(s) 414
AfeI AGCGCT 1 cut(s) 7042
AflII CTTAAG 1 cut(s) 2110
AflIII ACRYGT 5 cut(s) 3217, 3978, 4171, 5321, 5373
AhdI GACNNNNNGTC 1 cut(s) 1564
AhlI ACTAGT 2 cut(s) 3332, 4751
AjiI CACGTC 1 cut(s) 3218
AjuI GAANNNNNNNTTGG 4 cut(s) 664, 696, 2443, 2475
AleI CACNNNNGTG 1 cut(s) 6692
AloI GAACNNNNNNTCC 2 cut(s) 5671, 5703
Alw21I GWGCWC 2 cut(s) 1286, 6199
AlwI GGATC 6 cut(s) 4464, 4778, 5220, 6173, 6951, 7335
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
Aor51HI AGCGCT 1 cut(s) 7042
ArsI GACNNNNNNTTYG 2 cut(s) 4628, 4660
AseI ATTAAT 3 cut(s) 969, 4520, 7247
Asp700I GAANNNNTTC 3 cut(s) 3541, 4192, 7308
AspA2I CCTAGG 1 cut(s) 445
AspLEI GCGC 5 cut(s) 3133, 4227, 4363, 5187, 7043
AspS9I GGNCC 8 cut(s) 165, 573, 746, 909, 4306, 4919, 6820, 7061
AsuC2I CCSGG 2 cut(s) 3389, 3424
AsuII TTCGAA 4 cut(s) 438, 3042, 3913, 4707
AsuNHI GCTAGC 1 cut(s) 1669
AvaI CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
AvaII GGWCC 4 cut(s) 573, 4306, 6820, 7061
AvrII CCTAGG 1 cut(s) 445
AxyI CCTNAGG 2 cut(s) 1628, 3605
BaeI ACNNNNGTAYC 2 cut(s) 1487, 1520
BanII GRGCYC 2 cut(s) 1286, 6430
BauI CACGAG 1 cut(s) 841
BbrPI CACGTG 1 cut(s) 5324
BbsI GAAGAC 9 cut(s) 2032, 2448, 2775, 3220, 4082, 4638, 4853, 5764, 5853
Bbv12I GWGCWC 2 cut(s) 1286, 6199
BbvCI CCTCAGC 2 cut(s) 4107, 7121
BceAI ACGGC 2 cut(s) 1424, 2728
BcgI CGANNNNNNTGC 2 cut(s) 4079, 4113
BciVI GTATCC 3 cut(s) 125, 241, 4359
BclI TGATCA 3 cut(s) 2821, 3481, 5286
BcnI CCSGG 2 cut(s) 3389, 3424
BcuI ACTAGT 2 cut(s) 3332, 4751
BfmI CTRYAG 3 cut(s) 2672, 2763, 7336
BfoI RGCGCY 1 cut(s) 7044
BfrI CTTAAG 1 cut(s) 2110
BfuAI ACCTGC 3 cut(s) 4126, 7209, 7236
BfuI GTATCC 3 cut(s) 125, 241, 4359
BglI GCCNNNNNGGC 1 cut(s) 6777
BglII AGATCT 7 cut(s) 1289, 1304, 2355, 3559, 3926, 6589, 7332
BlnI CCTAGG 1 cut(s) 445
Bme18I GGWCC 4 cut(s) 573, 4306, 6820, 7061
BmeRI GACNNNNNGTC 1 cut(s) 1564
BmeT110I CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
BmgBI CACGTC 1 cut(s) 3218
BmgT120I GGNCC 8 cut(s) 165, 573, 746, 909, 4306, 4919, 6820, 7061
BmiI GGNNCC 4 cut(s) 2063, 3321, 5163, 6429
BmrI ACTGGG 2 cut(s) 253, 6730
BmtI GCTAGC 1 cut(s) 1673
BmuI ACTGGG 2 cut(s) 253, 6730
BpiI GAAGAC 9 cut(s) 2032, 2448, 2775, 3220, 4082, 4638, 4853, 5764, 5853
BplI GAGNNNNNCTC 4 cut(s) 4369, 4401, 6545, 6577
Bpu10I CCTNAGC 3 cut(s) 4107, 5432, 7121
Bpu14I TTCGAA 4 cut(s) 438, 3042, 3913, 4707
BpuEI CTTGAG 6 cut(s) 186, 1571, 3134, 3229, 3692, 6260
BpuMI CCSGG 2 cut(s) 3389, 3424
BsaAI YACGTR 4 cut(s) 1161, 4172, 5324, 5503
BsaBI GATNNNNATC 3 cut(s) 3656, 4997, 5835
BsaI GGTCTC 6 cut(s) 423, 1000, 1628, 1787, 6603, 6759
BsaWI WCCGGW 1 cut(s) 991
BsaXI ACNNNNNCTCC 8 cut(s) 2251, 2281, 3396, 3426, 4237, 4267, 6069, 6099
Bse118I RCCGGY 2 cut(s) 557, 911
Bse21I CCTNAGG 2 cut(s) 1628, 3605
Bse3DI GCAATG 9 cut(s) 592, 1353, 1410, 1929, 2192, 3340, 6078, 6391, 6465
Bse8I GATNNNNATC 3 cut(s) 3656, 4997, 5835
BseJI GATNNNNATC 3 cut(s) 3656, 4997, 5835
BseMI GCAATG 9 cut(s) 592, 1353, 1410, 1929, 2192, 3340, 6078, 6391, 6465
BseRI GAGGAG 3 cut(s) 92, 160, 3025
BseYI CCCAGC 1 cut(s) 6021
BsgI GTGCAG 4 cut(s) 36, 2422, 4299, 6984
Bsh1236I CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
Bsh1285I CGRYCG 1 cut(s) 1916
BsiEI CGRYCG 1 cut(s) 1916
BsiHKAI GWGCWC 2 cut(s) 1286, 6199
BsiHKCI CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
BsiSI CCGG 7 cut(s) 558, 912, 992, 3388, 3424, 4619, 5188
BslFI GGGAC 5 cut(s) 419, 1661, 6665, 6737, 7155
BsmBI CGTCTC 2 cut(s) 1821, 2044
BsmFI GGGAC 5 cut(s) 419, 1661, 6665, 6737, 7155
BsmI GAATGC 3 cut(s) 3166, 3704, 5197
Bso31I GGTCTC 6 cut(s) 423, 1000, 1628, 1787, 6603, 6759
BsoBI CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
Bsp119I TTCGAA 4 cut(s) 438, 3042, 3913, 4707
Bsp1286I GDGCHC 3 cut(s) 1286, 6199, 6430
Bsp1407I TGTACA 3 cut(s) 3780, 4174, 4953
Bsp19I CCATGG 2 cut(s) 3171, 4869
Bsp68I TCGCGA 1 cut(s) 1349
BspFNI CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
BspHI TCATGA 1 cut(s) 5038
BspLI GGNNCC 4 cut(s) 2063, 3321, 5163, 6429
BspMAI CTGCAG 2 cut(s) 2676, 2767
BspMI ACCTGC 3 cut(s) 4126, 7209, 7236
BspOI GCTAGC 1 cut(s) 1673
BspPI GGATC 6 cut(s) 4464, 4778, 5220, 6173, 6951, 7335
BspQI GCTCTTC 3 cut(s) 2733, 4314, 6204
BspT104I TTCGAA 4 cut(s) 438, 3042, 3913, 4707
BspTI CTTAAG 1 cut(s) 2110
BspTNI GGTCTC 6 cut(s) 423, 1000, 1628, 1787, 6603, 6759
BsrBI CCGCTC 1 cut(s) 865
BsrDI GCAATG 9 cut(s) 592, 1353, 1410, 1929, 2192, 3340, 6078, 6391, 6465
BsrFI RCCGGY 2 cut(s) 557, 911
BsrGI TGTACA 3 cut(s) 3780, 4174, 4953
BssAI RCCGGY 2 cut(s) 557, 911
BssNAI GTATAC 3 cut(s) 1960, 5953, 7365
BssSI CACGAG 1 cut(s) 841
BssT1I CCWWGG 4 cut(s) 445, 2422, 3171, 4869
Bst1107I GTATAC 3 cut(s) 1960, 5953, 7365
Bst2BI CACGAG 1 cut(s) 841
Bst6I CTCTTC 9 cut(s) 1272, 2733, 3972, 4244, 4314, 5037, 5966, 5981, 6204
BstAFI CTTAAG 1 cut(s) 2110
BstAPI GCANNNNNTGC 3 cut(s) 3191, 3929, 7354
BstAUI TGTACA 3 cut(s) 3780, 4174, 4953
BstBAI YACGTR 4 cut(s) 1161, 4172, 5324, 5503
BstBI TTCGAA 4 cut(s) 438, 3042, 3913, 4707
BstDSI CCRYGG 4 cut(s) 2887, 3171, 4869, 5197
BstEII GGTNACC 2 cut(s) 2891, 6452
BstENI CCTNNNNNAGG 1 cut(s) 802
BstFNI CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
BstH2I RGCGCY 1 cut(s) 7044
BstHHI GCGC 5 cut(s) 3133, 4227, 4363, 5187, 7043
BstMCI CGRYCG 1 cut(s) 1916
BstNSI RCATGY 5 cut(s) 3201, 3586, 3982, 5377, 7354
BstPI GGTNACC 2 cut(s) 2891, 6452
BstSFI CTRYAG 3 cut(s) 2672, 2763, 7336
BstSNI TACGTA 1 cut(s) 1161
BstUI CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
BstV2I GAAGAC 9 cut(s) 2032, 2448, 2775, 3220, 4082, 4638, 4853, 5764, 5853
BstXI CCANNNNNNTGG 4 cut(s) 1697, 2903, 3172, 5009
BstZ17I GTATAC 3 cut(s) 1960, 5953, 7365
Bsu36I CCTNAGG 2 cut(s) 1628, 3605
BsuI GTATCC 3 cut(s) 125, 241, 4359
BtgI CCRYGG 4 cut(s) 2887, 3171, 4869, 5197
BtrI CACGTC 1 cut(s) 3218
BtsI GCAGTG 5 cut(s) 507, 3185, 3918, 5994, 6454
BtuMI TCGCGA 1 cut(s) 1349
BveI ACCTGC 3 cut(s) 4126, 7209, 7236
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 5038
CfoI GCGC 5 cut(s) 3133, 4227, 4363, 5187, 7043
Cfr10I RCCGGY 2 cut(s) 557, 911
Cfr13I GGNCC 8 cut(s) 165, 573, 746, 909, 4306, 4919, 6820, 7061
CseI GACGC 4 cut(s) 2061, 5902, 7014, 7330
CsiI ACCWGGT 2 cut(s) 1560, 1962
DraI TTTAAA 3 cut(s) 3379, 6330, 6507
DraIII CACNNNGTG 1 cut(s) 414
DrdI GACNNNNNNGTC 2 cut(s) 4304, 5768
DriI GACNNNNNGTC 1 cut(s) 1564
DseDI GACNNNNNNGTC 2 cut(s) 4304, 5768
Eam1104I CTCTTC 9 cut(s) 1272, 2733, 3972, 4244, 4314, 5037, 5966, 5981, 6204
Eam1105I GACNNNNNGTC 1 cut(s) 1564
EarI CTCTTC 9 cut(s) 1272, 2733, 3972, 4244, 4314, 5037, 5966, 5981, 6204
EciI GGCGGA 1 cut(s) 5730
Ecl136II GAGCTC 1 cut(s) 1284
Eco105I TACGTA 1 cut(s) 1161
Eco130I CCWWGG 4 cut(s) 445, 2422, 3171, 4869
Eco147I AGGCCT 1 cut(s) 5543
Eco24I GRGCYC 2 cut(s) 1286, 6430
Eco31I GGTCTC 6 cut(s) 423, 1000, 1628, 1787, 6603, 6759
Eco32I GATATC 2 cut(s) 5313, 6841
Eco47I GGWCC 4 cut(s) 573, 4306, 6820, 7061
Eco47III AGCGCT 1 cut(s) 7042
Eco53kI GAGCTC 1 cut(s) 1284
Eco72I CACGTG 1 cut(s) 5324
Eco81I CCTNAGG 2 cut(s) 1628, 3605
Eco88I CYCGRG 5 cut(s) 815, 5555, 6581, 6593, 7358
Eco91I GGTNACC 2 cut(s) 2891, 6452
EcoICRI GAGCTC 1 cut(s) 1284
EcoNI CCTNNNNNAGG 1 cut(s) 802
EcoO109I RGGNCCY 1 cut(s) 746
EcoO65I GGTNACC 2 cut(s) 2891, 6452
EcoRI GAATTC 4 cut(s) 3015, 3541, 4192, 5391
EcoRV GATATC 2 cut(s) 5313, 6841
EcoT14I CCWWGG 4 cut(s) 445, 2422, 3171, 4869
EcoT22I ATGCAT 1 cut(s) 2383
EcoT38I GRGCYC 2 cut(s) 1286, 6430
ErhI CCWWGG 4 cut(s) 445, 2422, 3171, 4869
Esp3I CGTCTC 2 cut(s) 1821, 2044
FalI AAGNNNNNCTT 2 cut(s) 2039, 2071
FaqI GGGAC 5 cut(s) 419, 1661, 6665, 6737, 7155
FauI CCCGC 4 cut(s) 396, 3768, 4041, 7353
FauNDI CATATG 5 cut(s) 982, 1063, 2077, 5983, 7256
FbaI TGATCA 3 cut(s) 2821, 3481, 5286
FblI GTMKAC 6 cut(s) 210, 1959, 2635, 5952, 6810, 7364
FriOI GRGCYC 2 cut(s) 1286, 6430
GlaI GCGC 5 cut(s) 3132, 4226, 4362, 5186, 7042
GsaI CCCAGC 1 cut(s) 6025
HaeII RGCGCY 1 cut(s) 7044
HapII CCGG 7 cut(s) 558, 912, 992, 3388, 3424, 4619, 5188
HgaI GACGC 4 cut(s) 2061, 5902, 7014, 7330
HhaI GCGC 5 cut(s) 3133, 4227, 4363, 5187, 7043
Hin6I GCGC 5 cut(s) 3131, 4225, 4361, 5185, 7041
HinP1I GCGC 5 cut(s) 3131, 4225, 4361, 5185, 7041
HincII GTYRAC 2 cut(s) 4716, 6811
HindII GTYRAC 2 cut(s) 4716, 6811
HindIII AAGCTT 3 cut(s) 706, 6524, 6604
HpaII CCGG 7 cut(s) 558, 912, 992, 3388, 3424, 4619, 5188
Hpy99I CGWCG 1 cut(s) 1349
HpyCH4IV ACGT 7 cut(s) 1160, 1311, 3217, 4171, 4480, 5323, 5502
HpySE526I ACGT 7 cut(s) 1160, 1311, 3217, 4171, 4480, 5323, 5502
HspAI GCGC 5 cut(s) 3131, 4225, 4361, 5185, 7041
Ksp22I TGATCA 3 cut(s) 2821, 3481, 5286
LguI GCTCTTC 3 cut(s) 2733, 4314, 6204
MabI ACCWGGT 2 cut(s) 1560, 1962
MaeII ACGT 7 cut(s) 1160, 1311, 3217, 4171, 4480, 5323, 5502
MbiI CCGCTC 1 cut(s) 865
MfeI CAATTG 6 cut(s) 494, 1728, 1755, 1884, 2525, 3273
MhlI GDGCHC 3 cut(s) 1286, 6199, 6430
MlyI GAGTC 5 cut(s) 3001, 3421, 3512, 4261, 7137
MmeI TCCRAC 5 cut(s) 677, 1183, 3811, 5078, 6261
Mph1103I ATGCAT 1 cut(s) 2383
MroXI GAANNNNTTC 3 cut(s) 3541, 4192, 7308
MspA1I CMGCKG 2 cut(s) 50, 6640
MspCI CTTAAG 1 cut(s) 2110
MspI CCGG 7 cut(s) 558, 912, 992, 3388, 3424, 4619, 5188
MunI CAATTG 6 cut(s) 494, 1728, 1755, 1884, 2525, 3273
Mva1269I GAATGC 3 cut(s) 3166, 3704, 5197
MvnI CGCG 8 cut(s) 1232, 1349, 2366, 4012, 4225, 5185, 5913, 7274
NciI CCSGG 2 cut(s) 3389, 3424
NcoI CCATGG 2 cut(s) 3171, 4869
NdeI CATATG 5 cut(s) 982, 1063, 2077, 5983, 7256
NheI GCTAGC 1 cut(s) 1669
NlaIV GGNNCC 4 cut(s) 2063, 3321, 5163, 6429
NmuCI GTSAC 8 cut(s) 2966, 2993, 5356, 5989, 6075, 6694, 7112, 7238
NruI TCGCGA 1 cut(s) 1349
NsiI ATGCAT 1 cut(s) 2383
NspI RCATGY 5 cut(s) 3201, 3586, 3982, 5377, 7354
NspV TTCGAA 4 cut(s) 438, 3042, 3913, 4707
OliI CACNNNNGTG 1 cut(s) 6692
PaeR7I CTCGAG 3 cut(s) 815, 5555, 7358
PagI TCATGA 1 cut(s) 5038
PaqCI CACCTGC 1 cut(s) 4126
PasI CCCWGGG 1 cut(s) 2555
PceI AGGCCT 1 cut(s) 5543
PciI ACATGT 2 cut(s) 3978, 5373
PciSI GCTCTTC 3 cut(s) 2733, 4314, 6204
PcsI WCGNNNNNNNCGW 2 cut(s) 4905, 5633
PctI GAATGC 3 cut(s) 3166, 3704, 5197
PdmI GAANNNNTTC 3 cut(s) 3541, 4192, 7308
PflFI GACNNNGTC 1 cut(s) 5867
PflMI CCANNNNNTGG 5 cut(s) 260, 1359, 3631, 3830, 4274
Ple19I CGATCG 1 cut(s) 1916
PleI GAGTC 5 cut(s) 3000, 3421, 3511, 4260, 7137
PmaCI CACGTG 1 cut(s) 5324
PmlI CACGTG 1 cut(s) 5324
PpsI GAGTC 5 cut(s) 3000, 3421, 3511, 4260, 7137
Ppu21I YACGTR 4 cut(s) 1161, 4172, 5324, 5503
PscI ACATGT 2 cut(s) 3978, 5373
PshBI ATTAAT 3 cut(s) 969, 4520, 7247
PsiI TTATAA 1 cut(s) 4997
Psp124BI GAGCTC 1 cut(s) 1286
PspCI CACGTG 1 cut(s) 5324
PspEI GGTNACC 2 cut(s) 2891, 6452
PspFI CCCAGC 1 cut(s) 6021
PspN4I GGNNCC 4 cut(s) 2063, 3321, 5163, 6429
PspPI GGNCC 8 cut(s) 165, 573, 746, 909, 4306, 4919, 6820, 7061
PspXI VCTCGAGB 2 cut(s) 815, 7358
PsrI GAACNNNNNNTAC 4 cut(s) 6884, 6916, 7293, 7325
PstI CTGCAG 2 cut(s) 2676, 2767
PstNI CAGNNNCTG 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 5867
PvuI CGATCG 1 cut(s) 1916
PvuII CAGCTG 2 cut(s) 50, 6640
RruI TCGCGA 1 cut(s) 1349
SacI GAGCTC 1 cut(s) 1286
SalI GTCGAC 1 cut(s) 6809
SapI GCTCTTC 3 cut(s) 2733, 4314, 6204
Sau96I GGNCC 8 cut(s) 165, 573, 746, 909, 4306, 4919, 6820, 7061
SchI GAGTC 5 cut(s) 3001, 3421, 3512, 4261, 7137
SduI GDGCHC 3 cut(s) 1286, 6199, 6430
SexAI ACCWGGT 2 cut(s) 1560, 1962
SfcI CTRYAG 3 cut(s) 2672, 2763, 7336
Sfr274I CTCGAG 3 cut(s) 815, 5555, 7358
SfuI TTCGAA 4 cut(s) 438, 3042, 3913, 4707
SinI GGWCC 4 cut(s) 573, 4306, 6820, 7061
SlaI CTCGAG 3 cut(s) 815, 5555, 7358
SnaBI TACGTA 1 cut(s) 1161
SpeI ACTAGT 2 cut(s) 3332, 4751
SseBI AGGCCT 1 cut(s) 5543
SspI AATATT 2 cut(s) 884, 3762
SstI GAGCTC 1 cut(s) 1286
StuI AGGCCT 1 cut(s) 5543
StyI CCWWGG 4 cut(s) 445, 2422, 3171, 4869
TaiI ACGT 7 cut(s) 1163, 1314, 3220, 4174, 4483, 5326, 5505
TaqII GACCGA 1 cut(s) 4094
TatI WGTACW 7 cut(s) 2666, 2676, 3780, 4174, 4953, 5522, 7159
TauI GCSGC 3 cut(s) 868, 2369, 7277
TseFI GTSAC 8 cut(s) 2966, 2993, 5356, 5989, 6075, 6694, 7112, 7238
Tsp45I GTSAC 8 cut(s) 2966, 2993, 5356, 5989, 6075, 6694, 7112, 7238
TspGWI ACGGA 7 cut(s) 4087, 4245, 4314, 5331, 6178, 6896, 6965
Tth111I GACNNNGTC 1 cut(s) 5867
Van91I CCANNNNNTGG 5 cut(s) 260, 1359, 3631, 3830, 4274
Vha464I CTTAAG 1 cut(s) 2110
VpaK11BI GGWCC 4 cut(s) 573, 4306, 6820, 7061
VspI ATTAAT 3 cut(s) 969, 4520, 7247
XagI CCTNNNNNAGG 1 cut(s) 802
XceI RCATGY 5 cut(s) 3201, 3586, 3982, 5377, 7354
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XhoI CTCGAG 3 cut(s) 815, 5555, 7358
XmaJI CCTAGG 1 cut(s) 445
XmiI GTMKAC 6 cut(s) 210, 1959, 2635, 5952, 6810, 7364
XmnI GAANNNNTTC 3 cut(s) 3541, 4192, 7308
Zsp2I ATGCAT 1 cut(s) 2383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.