Rh6CG349700

Piezo non-specific cation channel, R-Ras-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006607
Physical Location & Seq
Forward (+)
53975886 .. 53988151
12266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG349700.1

Sequence Viewer

Length: 6177 bp
ATGGGCTATCGAGTGGAGTACAGCAGAAGAGTGGTGGGGAAAGCTCATTGGACTCTTGATATCTGGCGTAAGGCATACCAATGTCATGTTGACAGGGGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTGAGGGAGAGACCAAAGTATTGATTGTTGCCACAATAGCTTGGGGACTGCGCAAAAGCTCTCGGGCTATCATGCTGGCACTAATATTTCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTGTATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACGTTAACAGAAAATTACGCCAGTCTTTGATTCTCCTATGTGAGGTTCACTTTGCACTATTGTATATCATTCGGATCAATCCGATCTCCAGTAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCTTATTACAGTTAGGTCTCCTTGAAAAGGAAAGCTCGTGGGATTTCTTGGAAATAGCTTTGCTTGCTTGTTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTTTTTTCGTTCTCAGCAATCGTGCAACATACACCTAGCCTTCCATTTGGATTTGGCATTTTGAAAGCTGGTCTGAACAAATCAGTATTGTTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTATTCCTCAGTGCAATCGGGCAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAGACCAAATTATATCTCATTTGGGTACATATCTCTTCTCCTCGTTTGGATAATTGGAAGACAACTCGTTGAGAGAACGAAAAAGCGCTTGTGGTTCCCATTAAAAGCATATTCAATTACAGTATTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGTTTGGTTGTCCAGGTTGATAGACCTTGATTTCTATCTGGGGTATGACTCAAAAGCTTCGTCTTTGGAAAATGTTTGGGAATCCCTAGCAGTCTTGATTGTGATGCAACTTTACAGCTACGAGAGGAGGCAGAGCAGGTACAACAGGTCAGATGATCCTGATTGGACAGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTGTCTCCAATTTGTGCGTTCAGCTTTTTGTATCTCCTTGGCCTTGTCATCTGTTCAACTTTACCTAAAGCTTCACGAATCCCGTCTAAATCATTCTTGGCTTACACAGGATTTCTAGTAACAGCAGAGTATCTTTTTCAGATGTTGGGTAGCCAGGCTGCAATGTTTCCTGGACAAAAGCACTCTAATATTGCTCTTCTTTTGGGTTTCCGTGAATTTAAGGCTGGCTTCTGGGGTCTAGAATCTGGATTGAGAGGAAAAGTGCTGGTGATTGCTGCATGCACTCTTCAGTATAATGTTTTCCACTGGTTGGAGAAGATGCCAAGTACTATTCTAAGCAATGGAATGGGAGAGCCTTGTCCATTGTTTGTGTCAGCAGAAGATACAAATATTAGTGCTCCTATCCCTAGTGAGGATAACAGGCCATCAACTCCACTTTCTGTGAAGCGAGAAGCGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTGGTCTGTTGCAATCACTCAATCCCTCAACCCCAAAAGCAGGAAGTTCTGAGGGTAGTAGCGGTAGCAAATATTCATTTGGGTATATTTGGGGAAGCACCAAGGAGAGCCATAAGTGGAATACGAAAAAAATCCTTGCCTTGCGAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATGAAATTCTGGATGGAGAATTTGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCTCTGCTTCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATCGGCAGATTATACGCAAGTTATGGCCAATATTCGTCTTCCTGTTTGCTTCCATTCTCGTTCTTGAGTATTTTGCCATCTGGAAGAGTGCTTGGCCTCCAAATCATCCTGATGCAACTAATCCACATTGCCATGATTGCTGGCAAAAGTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTAATGTTGATGATCCTCGAATGCTTATCAGCTATTTTATTGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCGGGCTTATCAACGTACCGTGAGATGATATCTCAACGTGAAAATACATTTGTATGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTTTACTGCTATTGCCATCTACTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACCATTGAGTATGACATCCTGCACCTTGGTTATCTCGCTTTTGCACTGGTTTTCTTTCGGGTTAGACTTCAAATACTAAAGAAAAGGAACAAGATGTTCAAGTTCTTGCGCATATACAATTTTGCTCTCATTGTTCTATCTCTTGCATATCAATCTCCTTTTGTGGGTTGTTCTGGGAAGTGTGAGACCGTATATGAGATGATTGGCTTTTATAAATATGACTATGGGTTTCAGATGACTGCAAGATCAGCTCTCGTTGAAATTATCATATTTATGCTGGTATCACTTCAGTCATATATGTTTTCCTCTAAAGAGTTTGATTATGTGTCTCGATATCTTGAAGCTGAACAAATTGGTATGATTGTGCGCGAACAAGAAAAAAAGGCTGCATGGAAAACTGCACAATTACAACATGTTCGTGAATCTGAGGAGAAGAAACGCCAGCGTAACCTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCAGCTTCACAGCACGAACTCAGTTACTGACTGCGGTGACTCTTCTCCTGTCAGTGAAGGCCTAAGAAGGAGGAGAAGTACTTCTCTTAACTTGAACAATGATGCTTGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGAGACTTCTAGCAATGAATTGCATGAATCTCCTGCAACAGGGAATCTGGAAAACCCCACAGTTGTGGATTCTATGAAAAATTCAATGCAATCTATTCATTGTGAGATCACCGAAATTGAAGAGGACGTTGCTGATGGTACATTTTCTGATTCAGAGAAGAAAGAGAAGGGTAAAGGGAAATCAACGGATAACCCTCTAATATCAGCAGTTCATCTGATAGGTGATGGTGTTTCCCAGGTACAGTCTATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTGAATATTGACCAAGAATCCGATATCCATGAGCACTCTGCTGAGGATGGGGTATATGATGAGATGGAGAGCCAGAAAATAAAGTACGCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATGTGAGTTCTGATGCCACAAGTCTGCAGTTAGGAAGGATCTTCCGTCACATATGGTCCCGAATGCGGTCCAATAATGATGTTGTCTGTTATTGTTGCTTTGTCATCGTCTTTTTGTGGAACTTCAGTTTGCTTTCCATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTGTGTGTTAATTCTGGTCCAAGTTACATATTCTGGGTTATCATGCTCATATACACAGAAGTTTATATTTTGCTTCTGTATCTATACCAGATTATCATCCAGCACTATGGTACGCGTATTGCTTCAGACCTACTTCGTGAGTTGGGATTTCCTGCGCGTAAAATGTCTTCATTTGTTATCAGTTCGTTGCCCCTATTTCTCGTGTACTTATTCACCCTTGTCCAGAGCTCTATAACCGCAAAGGATGGCGAGTGGATGTCTTCAACAGACTTCAACTTCTATAGGAGGAACGCTTTGCAGGGAAAAGAGGTTCCTGTCAGTTATAGCTGGAGTGAGAGGGCAAAGGACCCGCTGCACATAATTGTAAATGTCATACAGTTGATATGTAGAAGCTTCTTTAGGTACTGGGTATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTGTTCAGGTTTCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCAGAAAGGATTGAGTCTGGAGTAAATCAATTGCTTAGAATCATCCATGATGAAAGATGCAAGGCAAAGGATCCTAAGCGATGCCCTTTTGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCGAGAAAATGCAAATGTGGCCTTGGTCGTTTTTGAGGTAGTATATGCCTCCCCCATAACAGACTGTGGTTCAGTAGAATGGTACAAGTCACTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCTTAAAGCACTGCATGCTGGCTTTGTTGAAGAAATAGGATTCCCATATCCTATACTCTCTGTTATCGGGGGAGGCAAAAGGGACATTGATCTGTTTGCCTATGTATTTGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTCCATTATAAAAAATAAAAGTGACTTTCTTGACGTGTATCAGCTTGAAGATCAATTTCCTAAAGAGTTCGTCTTCATCTTAATGATTATTTTTTTCTTGATTGTCCTTGATCGAATAATCTACCTCTGTTCATTTGCCACTGGAAAAGTGATTTACTACCTTTTTAACCTCATCCTATTCACATATTCAGTTACAAAGTATGCTTGGCATATGGAATCTTCCCGCCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAATCAATATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATTTCACGAATTAATTACTTAGGATATCGACTATATCGTGCTCTGCCATTCCTTTATGAATTGAGATGTGTACTTGACTGGTCATGTACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTCGTCAAATGTGATGCAGTCTTGAATAGAGCAACGCACAAACAAGGAGAAAAGCAAACACAAATGACCAAATGTTGCAATGGGATTTGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCCATTAAAGATGCCAGTGTTCAAGTTGATATCAAGACAATGAGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATTGATTGGGATAACGTCAACACTGATGTTAATCTTGACCCTCAAGGTTATTTGGAGACATATAACAAGAAAGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCTGATGTCGTTCAGACCAGATTCATACGGTCCCTCAATTGGGAGAGCAACATGGCTATAACTTTTACTTTGGTACTTTCCAGGGAGAGACCCAAAGGCAAGGAAGTTGTGAAATATGAAAATACTCTTGGTGTTGAGGATCTTCCAAAACAATCAGATGTACAAAAAGTCCTTAATGGCTCTATCAACAGCTTTAGGATACACAATGTCTATCCAAGATACTTGCGTGTCACAGGTTCTGGTGATGTCAGGCCACTGGAAGCAGGGGAAACTTTTGTCACTGCGGACCTCGTTATAAATCGCGCTAGTTACCAGTGGTGGTGGTCCTTCAGTGACATCAATTCATCAGATGTCAACGGATGTGGCGGTTTGAGAGGTCCCATGGCCATTATTATGTCTGAAGAAACGCCGCCACAGGGTATTCTTGGTGACACACTAAGCAAGTTCAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCCTATGAAAATCTTCCCTCCTGTGATAGGTTGTTGGCCATTTGTGAGGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGTATTGAAGAGATCCTTTACTGGACACTGGTGAAGATTTACAGATCACCACACATGCTGCTAGAGTACACAAAACCTGACTAG

Protein Analysis

2058

Amino Acids

235.94

Weight (kDa)

8.2

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIEZO PF25288 57 - 186 2.5e-63 Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1 PF23188 1201 - 1319 4.7e-15 Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor PF24874 1512 - 1749 1e-65 Piezo, THU9 and anchor domain
Piezo_cap PF12166 1777 - 2057 2.5e-76 Piezo non-specific cation channel, cap domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2691, 4607, 5777
Acc16I TGCGCA 2 cut(s) 197, 2588
Acc36I ACCTGC 2 cut(s) 1086, 2937
AccB7I CCANNNNNTGG 3 cut(s) 1522, 2443, 4187
AccII CGCG 4 cut(s) 2847, 3817, 3859, 5784
AccIII TCCGGA 1 cut(s) 106
AclI AACGTT 1 cut(s) 309
AclWI GGATC 9 cut(s) 389, 1109, 2225, 2454, 3578, 4262, 4275, 5628, 6100
AcoI YGGCCR 7 cut(s) 1685, 2051, 4178, 4584, 5865, 5968, 6048
AcsI RAATTY 7 cut(s) 1130, 1427, 1892, 1906, 3208, 4911, 4956
AcuI CTGAAG 8 cut(s) 98, 1484, 2750, 3640, 3810, 5794, 5901, 5969
AdeI CACNNNGTG 2 cut(s) 3707, 4850
AfeI AGCGCT 1 cut(s) 881
AflIII ACRYGT 3 cut(s) 2890, 3815, 4632
AjiI CACGTC 1 cut(s) 4633
AjnI CCWGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
AjuI GAANNNNNNNTTGG 2 cut(s) 5592, 5624
AleI CACNNNNGTG 1 cut(s) 3191
AloI GAACNNNNNNTCC 4 cut(s) 1895, 1927, 3996, 4028
Alw21I GWGCWC 7 cut(s) 1612, 3447, 3932, 4175, 4999, 5345, 5993
AlwI GGATC 9 cut(s) 389, 1109, 2225, 2454, 3578, 4262, 4275, 5628, 6100
AlwNI CAGNNNCTG 5 cut(s) 2196, 2999, 4427, 5720, 5934
Ama87I CYCGRG 2 cut(s) 207, 2311
Aor13HI TCCGGA 1 cut(s) 106
Aor51HI AGCGCT 1 cut(s) 881
ApoI RAATTY 7 cut(s) 1130, 1427, 1892, 1906, 3208, 4911, 4956
ArsI GACNNNNNNTTYG 4 cut(s) 1004, 1036, 2363, 2395
AseI ATTAAT 1 cut(s) 4968
Asp700I GAANNNNTTC 5 cut(s) 1678, 1910, 3052, 3099, 6024
AspLEI GCGC 6 cut(s) 198, 882, 2589, 2847, 3859, 5786
AsuNHI GCTAGC 1 cut(s) 4289
AvaI CYCGRG 2 cut(s) 207, 2311
BaeI ACNNNNGTAYC 2 cut(s) 5692, 5725
BalI TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
BamHI GGATCC 1 cut(s) 4267
BanII GRGCYC 2 cut(s) 3932, 5230
BarI GAAGNNNNNNTAC 4 cut(s) 423, 455, 3853, 3885
BauI CACGAG 2 cut(s) 469, 3902
BbsI GAAGAC 6 cut(s) 859, 2056, 2254, 3861, 3954, 4663
Bbv12I GWGCWC 7 cut(s) 1612, 3447, 3932, 4175, 4999, 5345, 5993
BbvCI CCTCAGC 1 cut(s) 3453
BciT130I CCWGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
BciVI GTATCC 2 cut(s) 4185, 5673
BclI TGATCA 1 cut(s) 2293
BfmI CTRYAG 4 cut(s) 931, 3524, 3557, 3982
BfoI RGCGCY 1 cut(s) 883
BfuAI ACCTGC 2 cut(s) 1086, 2937
BfuI GTATCC 2 cut(s) 4185, 5673
BglII AGATCT 1 cut(s) 1182
BmcAI AGTACT 3 cut(s) 1540, 2204, 3052
Bme1390I CCNGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
BmeT110I CYCGRG 2 cut(s) 207, 2311
BmgBI CACGTC 1 cut(s) 4633
BmrFI CCNGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
BmrI ACTGGG 1 cut(s) 4117
BmtI GCTAGC 1 cut(s) 4293
BmuI ACTGGG 1 cut(s) 4117
BpiI GAAGAC 6 cut(s) 859, 2056, 2254, 3861, 3954, 4663
BpmI CTGGAG 6 cut(s) 379, 4051, 4236, 4404, 4854, 5102
Bpu10I CCTNAGC 2 cut(s) 3453, 4272
BpuEI CTTGAG 4 cut(s) 958, 1949, 2111, 5385
BsaBI GATNNNNATC 3 cut(s) 993, 3797, 5388
BsaI GGTCTC 7 cut(s) 149, 455, 790, 2364, 2657, 5563, 5945
BsaWI WCCGGW 2 cut(s) 106, 143
BsaXI ACNNNNNCTCC 4 cut(s) 1895, 1925, 3034, 3064
Bse8I GATNNNNATC 3 cut(s) 993, 3797, 5388
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
BseJI GATNNNNATC 3 cut(s) 993, 3797, 5388
BseMII CTCAG 6 cut(s) 564, 718, 1740, 2895, 3006, 3444
BseRI GAGGAG 5 cut(s) 634, 824, 1099, 2921, 3058
BseYI CCCAGC 1 cut(s) 950
BsgI GTGCAG 4 cut(s) 447, 2483, 2862, 4040
Bsh1236I CGCG 4 cut(s) 2847, 3817, 3859, 5784
BsiHKAI GWGCWC 7 cut(s) 1612, 3447, 3932, 4175, 4999, 5345, 5993
BsiHKCI CYCGRG 2 cut(s) 207, 2311
BsiSI CCGG 2 cut(s) 107, 144
BslFI GGGAC 6 cut(s) 204, 3574, 4120, 4541, 5497, 5844
BsmFI GGGAC 6 cut(s) 204, 3574, 4120, 4541, 5497, 5844
BsmI GAATGC 3 cut(s) 1981, 2244, 3600
Bso31I GGTCTC 7 cut(s) 149, 455, 790, 2364, 2657, 5563, 5945
BsoBI CYCGRG 2 cut(s) 207, 2311
Bsp1286I GDGCHC 8 cut(s) 1612, 3447, 3932, 4175, 4999, 5230, 5345, 5993
Bsp13I TCCGGA 1 cut(s) 106
Bsp1407I TGTACA 3 cut(s) 4303, 5042, 5641
Bsp19I CCATGG 3 cut(s) 523, 3669, 5862
BspCNI CTCAG 6 cut(s) 563, 717, 1741, 2896, 3005, 3445
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 4 cut(s) 2847, 3817, 3859, 5784
BspMAI CTGCAG 2 cut(s) 3528, 3561
BspMI ACCTGC 2 cut(s) 1086, 2937
BspOI GCTAGC 1 cut(s) 4293
BspPI GGATC 9 cut(s) 389, 1109, 2225, 2454, 3578, 4262, 4275, 5628, 6100
BspQI GCTCTTC 1 cut(s) 1413
BspTNI GGTCTC 7 cut(s) 149, 455, 790, 2364, 2657, 5563, 5945
BsrGI TGTACA 3 cut(s) 4303, 5042, 5641
BssSI CACGAG 2 cut(s) 469, 3902
BssT1I CCWWGG 8 cut(s) 523, 1249, 1800, 2460, 2503, 3669, 4344, 5862
Bst2BI CACGAG 2 cut(s) 469, 3902
Bst2UI CCWGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
Bst6I CTCTTC 8 cut(s) 22, 834, 1413, 1503, 2105, 3019, 3243, 6096
BstAPI GCANNNNNTGC 5 cut(s) 1964, 2006, 2205, 4460, 4850
BstAUI TGTACA 3 cut(s) 4303, 5042, 5641
BstDSI CCRYGG 3 cut(s) 523, 3669, 5862
BstEII GGTNACC 1 cut(s) 5252
BstENI CCTNNNNNAGG 4 cut(s) 347, 458, 3165, 6037
BstFNI CGCG 4 cut(s) 2847, 3817, 3859, 5784
BstH2I RGCGCY 1 cut(s) 883
BstHHI GCGC 6 cut(s) 198, 882, 2589, 2847, 3859, 5786
BstNI CCWGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
BstNSI RCATGY 8 cut(s) 1494, 2398, 2894, 4304, 4463, 4832, 5096, 6151
BstPI GGTNACC 1 cut(s) 5252
BstSCI CCNGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
BstSFI CTRYAG 4 cut(s) 931, 3524, 3557, 3982
BstUI CGCG 4 cut(s) 2847, 3817, 3859, 5784
BstV2I GAAGAC 6 cut(s) 859, 2056, 2254, 3861, 3954, 4663
BstX2I RGATCY 6 cut(s) 1182, 2446, 3570, 4267, 5620, 6105
BstXI CCANNNNNNTGG 4 cut(s) 403, 3193, 3809, 4832
BstYI RGATCY 6 cut(s) 1182, 2446, 3570, 4267, 5620, 6105
BsuI GTATCC 2 cut(s) 4185, 5673
BtgI CCRYGG 3 cut(s) 523, 3669, 5862
BtgZI GCGATG 1 cut(s) 4291
BtrI CACGTC 1 cut(s) 4633
BtsI GCAGTG 4 cut(s) 4454, 4883, 5254, 5760
BveI ACCTGC 2 cut(s) 1086, 2937
CaiI CAGNNNCTG 5 cut(s) 2196, 2999, 4427, 5720, 5934
CfoI GCGC 6 cut(s) 198, 882, 2589, 2847, 3859, 5786
CsiI ACCWGGT 1 cut(s) 250
CspCI CAANNNNNGTGG 2 cut(s) 5824, 5859
DraIII CACNNNGTG 2 cut(s) 3707, 4850
EaeI YGGCCR 7 cut(s) 1685, 2051, 4178, 4584, 5865, 5968, 6048
Eam1104I CTCTTC 8 cut(s) 22, 834, 1413, 1503, 2105, 3019, 3243, 6096
EarI CTCTTC 8 cut(s) 22, 834, 1413, 1503, 2105, 3019, 3243, 6096
Ecl136II GAGCTC 1 cut(s) 3930
Eco130I CCWWGG 8 cut(s) 523, 1249, 1800, 2460, 2503, 3669, 4344, 5862
Eco147I AGGCCT 1 cut(s) 3033
Eco24I GRGCYC 2 cut(s) 3932, 5230
Eco31I GGTCTC 7 cut(s) 149, 455, 790, 2364, 2657, 5563, 5945
Eco32I GATATC 6 cut(s) 61, 2340, 2813, 3436, 4982, 5305
Eco47III AGCGCT 1 cut(s) 881
Eco53kI GAGCTC 1 cut(s) 3930
Eco57I CTGAAG 8 cut(s) 98, 1484, 2750, 3640, 3810, 5794, 5901, 5969
Eco88I CYCGRG 2 cut(s) 207, 2311
Eco91I GGTNACC 1 cut(s) 5252
EcoICRI GAGCTC 1 cut(s) 3930
EcoNI CCTNNNNNAGG 4 cut(s) 347, 458, 3165, 6037
EcoO109I RGGNCCY 2 cut(s) 4048, 5858
EcoO65I GGTNACC 1 cut(s) 5252
EcoRI GAATTC 1 cut(s) 4911
EcoRII CCWGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
EcoRV GATATC 6 cut(s) 61, 2340, 2813, 3436, 4982, 5305
EcoT14I CCWWGG 8 cut(s) 523, 1249, 1800, 2460, 2503, 3669, 4344, 5862
EcoT22I ATGCAT 1 cut(s) 1210
EcoT38I GRGCYC 2 cut(s) 3932, 5230
ErhI CCWWGG 8 cut(s) 523, 1249, 1800, 2460, 2503, 3669, 4344, 5862
FalI AAGNNNNNCTT 6 cut(s) 1424, 1456, 1951, 1983, 6093, 6125
FaqI GGGAC 6 cut(s) 204, 3574, 4120, 4541, 5497, 5844
FauI CCCGC 2 cut(s) 4059, 4829
FauNDI CATATG 3 cut(s) 134, 3584, 4809
FbaI TGATCA 1 cut(s) 2293
FriOI GRGCYC 2 cut(s) 3932, 5230
FspI TGCGCA 2 cut(s) 197, 2588
GlaI GCGC 6 cut(s) 197, 881, 2588, 2846, 3858, 5785
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 6 cut(s) 379, 4051, 4236, 4404, 4854, 5102
HaeII RGCGCY 1 cut(s) 883
HapII CCGG 2 cut(s) 107, 144
HhaI GCGC 6 cut(s) 198, 882, 2589, 2847, 3859, 5786
Hin6I GCGC 6 cut(s) 196, 880, 2587, 2845, 3857, 5784
HinP1I GCGC 6 cut(s) 196, 880, 2587, 2845, 3857, 5784
HincII GTYRAC 7 cut(s) 91, 313, 2178, 3394, 5328, 5377, 5836
HindII GTYRAC 7 cut(s) 91, 313, 2178, 3394, 5328, 5377, 5836
HindIII AAGCTT 5 cut(s) 1014, 1281, 1960, 4093, 5098
HpaI GTTAAC 2 cut(s) 313, 3394
HpaII CCGG 2 cut(s) 107, 144
HpyCH4IV ACGT 6 cut(s) 309, 2324, 2347, 3255, 4632, 5373
HpySE526I ACGT 6 cut(s) 309, 2324, 2347, 3255, 4632, 5373
HspAI GCGC 6 cut(s) 196, 880, 2587, 2845, 3857, 5784
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 2293
KspAI GTTAAC 2 cut(s) 313, 3394
LguI GCTCTTC 1 cut(s) 1413
LmnI GCTCC 6 cut(s) 406, 1615, 3115, 4127, 4178, 5233
MabI ACCWGGT 1 cut(s) 250
MaeII ACGT 6 cut(s) 309, 2324, 2347, 3255, 4632, 5373
MfeI CAATTG 2 cut(s) 4226, 5518
MflI RGATCY 6 cut(s) 1182, 2446, 3570, 4267, 5620, 6105
MhlI GDGCHC 8 cut(s) 1612, 3447, 3932, 4175, 4999, 5230, 5345, 5993
MlsI TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
MluI ACGCGT 1 cut(s) 3815
MluNI TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
MlyI GAGTC 6 cut(s) 46, 1001, 3005, 4142, 4220, 4330
MmeI TCCRAC 1 cut(s) 1503
Mox20I TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
Mph1103I ATGCAT 1 cut(s) 1210
MroI TCCGGA 1 cut(s) 106
MroXI GAANNNNTTC 5 cut(s) 1678, 1910, 3052, 3099, 6024
MscI TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
Msp20I TGGCCA 6 cut(s) 1687, 2053, 4180, 4586, 5867, 6050
MspA1I CMGCKG 2 cut(s) 4054, 4427
MspI CCGG 2 cut(s) 107, 144
MspR9I CCNGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
MunI CAATTG 2 cut(s) 4226, 5518
Mva1269I GAATGC 3 cut(s) 1981, 2244, 3600
MvaI CCWGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
MvnI CGCG 4 cut(s) 2847, 3817, 3859, 5784
NcoI CCATGG 3 cut(s) 523, 3669, 5862
NdeI CATATG 3 cut(s) 134, 3584, 4809
NheI GCTAGC 1 cut(s) 4289
NmuCI GTSAC 8 cut(s) 3008, 3578, 4410, 4619, 5710, 5758, 5813, 5909
NsbI TGCGCA 2 cut(s) 197, 2588
NsiI ATGCAT 1 cut(s) 1210
NspI RCATGY 8 cut(s) 1494, 2398, 2894, 4304, 4463, 4832, 5096, 6151
OliI CACNNNNGTG 1 cut(s) 3191
PaeI GCATGC 2 cut(s) 1494, 4463
PceI AGGCCT 1 cut(s) 3033
PciI ACATGT 1 cut(s) 2890
PciSI GCTCTTC 1 cut(s) 1413
PcsI WCGNNNNNNNCGW 1 cut(s) 4990
PctI GAATGC 3 cut(s) 1981, 2244, 3600
PdmI GAANNNNTTC 5 cut(s) 1678, 1910, 3052, 3099, 6024
PflMI CCANNNNNTGG 3 cut(s) 1522, 2443, 4187
PfoI TCCNGGA 1 cut(s) 1381
PleI GAGTC 6 cut(s) 46, 1001, 3005, 4141, 4219, 4329
PpsI GAGTC 6 cut(s) 46, 1001, 3005, 4141, 4219, 4329
PpuMI RGGWCCY 2 cut(s) 4048, 5858
PscI ACATGT 1 cut(s) 2890
PshBI ATTAAT 1 cut(s) 4968
PsiI TTATAA 3 cut(s) 2691, 4607, 5777
Psp124BI GAGCTC 1 cut(s) 3932
Psp1406I AACGTT 1 cut(s) 309
Psp5II RGGWCCY 2 cut(s) 4048, 5858
Psp6I CCWGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
PspEI GGTNACC 1 cut(s) 5252
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
PspPPI RGGWCCY 2 cut(s) 4048, 5858
PstI CTGCAG 2 cut(s) 3528, 3561
PstNI CAGNNNCTG 5 cut(s) 2196, 2999, 4427, 5720, 5934
PsuI RGATCY 6 cut(s) 1182, 2446, 3570, 4267, 5620, 6105
PvuII CAGCTG 1 cut(s) 4427
SacI GAGCTC 1 cut(s) 3932
SapI GCTCTTC 1 cut(s) 1413
ScaI AGTACT 3 cut(s) 1540, 2204, 3052
SchI GAGTC 6 cut(s) 46, 1001, 3005, 4142, 4220, 4330
ScrFI CCNGG 8 cut(s) 252, 973, 1367, 1383, 1701, 3365, 4177, 5563
SduI GDGCHC 8 cut(s) 1612, 3447, 3932, 4175, 4999, 5230, 5345, 5993
SexAI ACCWGGT 1 cut(s) 250
SfcI CTRYAG 4 cut(s) 931, 3524, 3557, 3982
SmlI CTYRAG 4 cut(s) 937, 1928, 2090, 5400
SmoI CTYRAG 4 cut(s) 937, 1928, 2090, 5400
SphI GCATGC 2 cut(s) 1494, 4463
SseBI AGGCCT 1 cut(s) 3033
SspI AATATT 6 cut(s) 231, 1402, 1603, 1772, 2058, 3418
SstI GAGCTC 1 cut(s) 3932
StuI AGGCCT 1 cut(s) 3033
StyD4I CCNGG 8 cut(s) 250, 971, 1365, 1381, 1699, 3363, 4175, 5561
StyI CCWWGG 8 cut(s) 523, 1249, 1800, 2460, 2503, 3669, 4344, 5862
TaiI ACGT 6 cut(s) 312, 2327, 2350, 3258, 4635, 5376
TaqI TCGA 6 cut(s) 10, 2236, 2809, 4324, 4711, 4984
TauI GCSGC 3 cut(s) 1670, 5893, 5973
TseFI GTSAC 8 cut(s) 3008, 3578, 4410, 4619, 5710, 5758, 5813, 5909
Tsp45I GTSAC 8 cut(s) 3008, 3578, 4410, 4619, 5710, 5758, 5813, 5909
TspGWI ACGGA 5 cut(s) 1412, 3329, 3566, 4838, 5853
Van91I CCANNNNNTGG 3 cut(s) 1522, 2443, 4187
VspI ATTAAT 1 cut(s) 4968
XagI CCTNNNNNAGG 4 cut(s) 347, 458, 3165, 6037
XapI RAATTY 7 cut(s) 1130, 1427, 1892, 1906, 3208, 4911, 4956
XbaI TCTAGA 1 cut(s) 1450
XceI RCATGY 8 cut(s) 1494, 2398, 2894, 4304, 4463, 4832, 5096, 6151
XcmI CCANNNNNNNNNTGG 1 cut(s) 3676
XmnI GAANNNNTTC 5 cut(s) 1678, 1910, 3052, 3099, 6024
ZrmI AGTACT 3 cut(s) 1540, 2204, 3052
Zsp2I ATGCAT 1 cut(s) 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.