Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0006607

Physical Location & Seq

Genomic Coordinates Reverse (-)

14997952 .. 15010924

Gene Structure 12973 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012072

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7413 bp

ATGGGCAGATTTCTTGGTGGGTTTTTGTTGCCTCTGCTGCTGCTCGCAGCTGCTTTCCTGCACTGGAGCTTGATCTCTCTTGGTGATCTCATATCTTTTCTTCTTATTCTATTTCATGCACCCAACATAGGATTTCATTTTGGAAGGAGGCTTTTATTGTTGTGGTTCATCATCATTTTTTCTTTATGTGTTATTTTTTCTGAAGTTCTATATCTGGTTCTATGGGCTATCGAGTGGAGTATAGCAGAAGAGTGGTGGGGAAAGCTCATTGGACTCTTGATAGCTGAGTCATGGAAATCACCATCTGTGGTATACTTTATGGTCCTTCAACTCTCCATAGTTGCTCTTTCATTGGTTGATTTGTATGGGAACAGATTTGGTCTTTTTTCATCATTTGATTCATGTTGGGGGCAATTCTTATCAACTGTTGATCGCTTAGTCTGTTCTCATATAAGGGTTGCTTCTTGCTTGCTGTTGCCTGCTATTCAACTGGTTGTTGGAATCAGCCATCCCTCATGGGTTTCTCTACCATTTTTTATTGGGAGCTGTGTTGGTCTTGTGGATTGGTCCTTGACAAGCAATTTTCTAGGACTTTTCAGGTGGTGGAGGCCTTTTCACTTGTATGCAGGCTTCAACATTGTCTTGCTTTATCTGTATCAGCTCCCTGTGGAGTTTCCAGATATGTTACAATGGGTAGCTGATTTCATTGGTCTCTTCAAAATAACTTCAGGTTCCCAATGGACCGAAGTTTGTTCAAGCTTCTCTCTTGTACTTTTTTACATAATGTTATCCTGTGTTAAATGTGATCTAGAGGAGATGGATTTTATTTTGTCTATGAAAGAAAATAACTTGACGGAGCATCTTCTTCCCTCAAAGCATTCATTTTTTATTCGTCAATCTAGATCTGGCGTAAGGCATACCAATGTCATGTTGACAGGGGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTATCCTTGGTTGCTCTTTCCTTCTGGAGTTTTCATTTTGCAAGTGTTTGTGCTTTTGGGCTACTTGCATATGTTGGCTACATCATATATGCATTCCCTTCCTTATTCCGTTTGCATCGACTGAATGGGCTGCTTCTTGTCTTCATTCTCTTGTGGGCTGTGAGCACATATATCTTCAATGTAGCTTTCACATTCTTGAATTGGAAAATTGGGAAGAACATGGACATATGGGAGATGGTTGGCTTGTGGCATTATCCCATACCTGGATTCTTTTTGCTTGCACAATTTTGTCTTGGAATGTTGGTTGCTTTGGGTAATCTTGTGAATAACTCAGTTTTCCTTTGCTTGTCTGATGAGGATGGGCGATCATCAACTGACAATAGTACTATTGAAGGTGAGGGAGAGACCAAAGTTTTGATTGTTGCCACAATAGCTTGGGGACTGCGCAAAAGCTCTCGGGCTATCATGCTGGCGCTAATATTTCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTGTATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACGTTAACAGAAAATTACGCCAGTCTTTGATTCTCCTATGCGAGGTTCACTTTGCACTATTGTATATCATTCGGATCAATCCGATCTCCAGTAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCTTATTACAGTTAGGTCTCCTTGAAAAGGAAAGCTTGTGGGATTTCTTGGAAATAGCTTTGCTTGCTTGTTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTTTTTTCGTTCTCAGCAATCGTGCAACATACACCTAGCCTTCCATTTGGATTTGGCATTTTGAAAGCTGGTCTGAATAAATCAGTATTGTTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTATTCCTCAGTGCAATCGGGCAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAAACCGAATTATATCTCATTTGGGTACATATCTCTTCTCCTCGTTTGGATAATTGGAAGACAACTCGTTGAGAGAACGAAAAAGCGCTTGTGGTTCCCATTAAAAGCATATTCAATTACAGTATTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGTTTGGTTGTCCAGGTTGATAGACCTTGATTTCTATCTGGGGTATGACTCAAAAGCTTCGTCTTTGGAAAATATTTGGGAATCCCTAGCAGTCTTGATTGTGATGCAACTTTACAGCTACGAGAGGAGGCAGAGCAGGTACAACAGGTCAGATGATCCTGATTTGACAGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTGTCTCCAATTTGTGCGTTTAGCTTTTTGTATCTCCTTGGCCTTGTCATCTGTTCAACTTTACCTAAAGCTTCACGAATCCCGTCTAAATCATTCTTGGCTTACACAGGATTTCTAGTAACAGCAGAGTATCTTTTTCAGATGTTGGGTAGCCAGGCTGCAATGTTTCCTGGACAAAAGCACTCTAATATTGCTCTTCTTTTGGGTTTCTGTGAATTTAAGGCTGGCTTCTGGGGTCTAGAATCTGGATTGAGAGGAAAAGTGCTGGTGATTGCTGCATGCACTCTTCAGTATAATGTTTTCCGCTGGTTGGAGAAGATGCCAAGTACTATTCTAAGCAATGGAATGGGAGAGCCTTGTCCTTTGTTTGTGTCAGCAGAAGATACAAATATTAGTGCTCCTACCCCTAGTGAGGATAACAGGCCATCAACTCCACTTTCTGTGAAGCGAGAAGCGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTGGTCTGTTGCAATCACTCAATCCCTCAACCCCAAAAGCAGGAAGTTCTGAGCGTAGTAGCGGTAGCAAATATTCATTTGGGTATATTTGGGGAAGCACCAAGGAGAGCCATAAGTGGAATACGAAAAAAATCCTTGCCTTGCGAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATGAAATTCTGGATGGAGAATTTGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCTCTGCTTCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATCGGCAGATTATACGCAAGTTATGGCCAATATTCGTCTTCCTGTTTGCTTCCATTCTCGTTCTTGAGTATTTTGCCATCTGGAAGAGTGCTTGGCCTCCAAATCATCCTGATGCAACTAATCCACGTTGCCATGATTGCTGGCAAAAGTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTAATGTTGATGATCCTCGAATGCTTATCAGCTATTTTATGGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCGGGCTTATCAACGTACCGTGAGATGATATCTCAACGTGAAAATACATTTGTATGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTTTACTGCTATTGCCATCTACTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACCATTGAGTATGACATCCTGCACCTTGGTTATCTCGCTTTTGCACTGGTTTTCTTTCGGGTTAGACTTCAAATACTAAAGAAAAGGAACAAGATGTTCAAGTACTTGCGCATATACAATTTTGCTCTCATTGTTCTATCTCTTGCATATCAATCTCCTTTTGTGGGTTGTTCTGGGAAGTGTGAGACCATATACGAGATGATTGGCTTTTATAAATATGACTATGGGTTTCAGATGACTGCAAGATCAGCTCTCGTTGAGATTATCATATTTATGCTGGTATCACTTCAGTCATATATGTTTTCCTCTAAAGAGTTTGATTATGTGTCTCGATATCTTGAAGCTGAACAAATTGGTATGATTGTGCGCGAACAAGAAAAAAAAGCTGCATGGAAAACTGCACAATTACAACATATTCGTGAATCTGAGGAGAAGAAACGCCAGCGTAACCTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCAGCTTCACAGCACGAACTCAGTTACTGACTGCGGTGACTCTTCTCCTGTCAGTGAAGGCCTAAGAAGGAGAAGAAGTGCTTCTCTTAACTTGAACAATGATGCTTGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGAGACTTCTAGCAATGAATTGCATGAATCTCCTGCAACAGGGAATCTGGAAAACCACACAGTTGGGGATTCTATGAAAAATTCAATGCAATCTATTCATTGTGAGATCACCGAAATTGACGAGGACGTTGCTGATGGTACATTTTCTGATTCAGAGAAGAAAGAGAAGGGTAAAGGGAAATCAACGGATAACCCTCTAATATCAGCAGTTCATCTGATTGGTGATGGTGTTTCCCAGGTACAGTCCATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTGAATATTGACCAAGAATCCGATATCCATGAGCACTCTGCTGAGGATGGGGTATATGATGAGATGGAGAGCCAGAAAATAAAGTACGCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATATGAGTTCTGATGCCACAAGTCTGCAGTTAGGAAGGATCTTCCGTCACATATGGTCCCGAATGCGGTCCAATAATGATGTTGTCTGTTATTGTTGCTTTGTCATCGTGTTTTTGTGGAACTTCAGTTTGCTTTCCATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTGTGTGTTAATTCTGGTCCAAGTTACATATTCTGGGTTATCATGCTCATATACACAGAAGTTTATATTTTGCTTCTGTATCTATACCAGATTATCATCCAGCACTATGGTTCGGGTATTGCTTCAGACCTACTTCGTGAGTTGGGATTTCCTGCACGTAAAATGTCTTCATTTGTTGTCAGTTCGTTGCCCCTATTTCTTGTGTACTTATTCACCCTTGTCCAGAGCTCTATAACCGCAAAGGATGGCGAGTGGATGTCTTCAACAGACTTCAACTTCTATAGGAGGAACGCTTTGCAGGGAAAAGAGGTTCCTGTCGGTTATAGCTGGAGTGAGAGGGCAAAGGACCTGCTGCACATAATTGTAAATGTCATACAGTTGATATGTAGAAGCTTCTTTAGGTACTGGGTATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTGTTCAGGTTTCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCAGAAAGGATTGAGTCTGGAGTAAATCAATTGCTTAGAATCATCCATGATGAAAGATGCAAGGCAAAGGATCCTAAGCGATGCCCTTTTGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCGAGAAAATGCAAATGTGGCCTTGGTCGTTTTTGAGGTAGTTTATGCCTCCCCCATAACAGACTGTGCTTCGGTAGAATGGTACAAGTCAGTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCTTAAAGCACTTCATGCTGGCTTTGTTGAAGAAATAGGATTCCCATATCCTATACTCTCTGTTATCGGGGGAGGCAAAAGGGACATTGATCTGTTTGCCTATGTATTCGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTCCATTATAAAAAATAAAAGTGACTTTCTTGACGTGTATCAGCTTGAAGATCAATTTCCTAAAGAGTTCGTCTTCATCTTAATGATTATTTTTTTCTTGATTGTCCTTGATCGAATAATCTACCTCTGTTCATTTGCCACCGGAAAAGTGATTTACTACCTTTTTAACCTCATCCTCTTCACGTATTCAGTTACAAAGTATGCTTGGCATATGGAATCTTCCCGCCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAATCAATATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATTTCACGAATTAATTACTTAGGATATCGACTATACCGTGCTCTGCCGTTCCTTTATGAATTGAGATGTGTACTTGACTGGTCATGTACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTCGTCAAATGTGATGCAGTCCTGAATAGAGCAACACACAAACAAGGAGAAAAGCAAACACAAATGACCAAATGTTGCAATGGGATTTGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCCATTAAAGATGCCAGTGTTCAAGTTGATATCAAGACAATGAGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATTGATTGGGATGACGTCAACTCTGATGTTAATCTTGACCCTCAAGGTTATTTGGAGACATATAACAAGAAAGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCTGATGTCGTTCAGACCAGATTCATACGGTCCCTCAATTGGGAGAGCAACATGGCTATAACTTTTACTTGGGTACTTTCCAGGGAGAGACCCAAAGGCAAGGAAGTTGTGAAATATGATAATACTCTTGGTTTTGAGGATCTTCCAAAACAATCAGATGTACAAAAAGTCCTTAATGGCTCTATCAACAGCTTTAGGATACACAATGTCTATCCAAGATACTTGCGTGTCACAGGTTCTGGTGATGTCAGGCCACTGGAACCAGGGGAAGCTTTCGTCACTGCAGACCTCATTATAAATCGTGCTAGTTACCAGTGGTGGTGGTCCTTCAGTGACATCAATTCATCAGATGTCAACGGATGTGGCGGTTTGAGAGGACCCATGGCCATTATTATGTCTGAAGAAACGCCGCCACAGGGTATTCTTGGTGACACACTAAGCAAGTTTAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCCTATGAAAATCTTCCCTCCTGTGATAGGTTGTTGGCCATTTGTGAGGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGTATTGAAGAGATCCTTTACTGGACACTGGTGAAGATTTACAGATCACCACACATGCTGCTAGAGTACACAAAACCTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0015075 GO:0015267 GO:0015318 GO:0016020 GO:0022803 GO:0022836 GO:0022838 GO:0022857 GO:0022890 GO:0034220 GO:0042391 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0065007 GO:0065008 GO:0071214 GO:0071260 GO:0071496 GO:0098655 GO:0104004

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Piezo_RRas_bdg

Protein Analysis

2471

Amino Acids

282.57

Weight (kDa)

7.65

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIEZO	PF25288	469 - 598	7.8e-63	Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1	PF23188	1613 - 1731	4.4e-15	Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor	PF24874	1924 - 2161	1.3e-65	Piezo, THU9 and anchor domain
Piezo_cap	PF12166	2189 - 2469	4.7e-77	Piezo non-specific cation channel, cap domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006607)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48060 AT2G48060 AT2G48060
fragaria_vesca	FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180
malus_domestica	MD03G1271800.v1.1 MD11G1292400.v1.1
prunus_persica	Prupe.8G248000_v2.0.a1 Prupe.8G248000_v2.0.a1
pyrus_communis	pycom03g21640 pycom11g25880
rosa_chinensis	RchiOBHm_Chr6g0291991
rosa_laevigata	RLG00000012072
rosa_multiflora	Rmu_sc0008666.1_g000011
rosa_roxburghii	Rroxscaffold_7G00175510 Rroxscaffold_7G00178010
rosa_rugosa	Rorug06G0222200 Rorug06G0222300
rosa_samantha	Rh6AG336100 Rh6BG343500 Rh6CG349700 Rh6DG336500
rosa_wichuraiana	Rw6G029260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	3927, 5843, 7013
AatII	GACGTC	1 cut(s)	6612
Acc16I	TGCGCA	2 cut(s)	1433, 3824
Acc36I	ACCTGC	3 cut(s)	2322, 4173, 5295
AccB7I	CCANNNNNTGG	2 cut(s)	3679, 5423
AccI	GTMKAC	1 cut(s)	312
AccII	CGCG	1 cut(s)	4083
AccIII	TCCGGA	1 cut(s)	948
AclI	AACGTT	1 cut(s)	1545
AclWI	GGATC	9 cut(s)	1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864, 7336
AcoI	YGGCCR	7 cut(s)	2921, 3287, 5414, 5820, 7101, 7204, 7284
AcsI	RAATTY	7 cut(s)	2366, 2663, 3128, 3142, 4444, 6147, 6192
AcyI	GRCGYC	1 cut(s)	6609
AdeI	CACNNNGTG	2 cut(s)	4943, 6086
AfeI	AGCGCT	1 cut(s)	2117
AflIII	ACRYGT	1 cut(s)	5868
AjiI	CACGTC	1 cut(s)	5869
AloI	GAACNNNNNNTCC	4 cut(s)	3131, 3163, 5232, 5264
Alw21I	GWGCWC	8 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6581, 7229
AlwI	GGATC	9 cut(s)	1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864, 7336
AlwNI	CAGNNNCTG	4 cut(s)	3432, 4235, 5663, 6956
Ama87I	CYCGRG	2 cut(s)	1443, 3547
Aor13HI	TCCGGA	1 cut(s)	948
Aor51HI	AGCGCT	1 cut(s)	2117
ApoI	RAATTY	7 cut(s)	2366, 2663, 3128, 3142, 4444, 6147, 6192
ArsI	GACNNNNNNTTYG	6 cut(s)	1384, 1416, 2240, 2272, 3599, 3631
AseI	ATTAAT	1 cut(s)	6204
Asp700I	GAANNNNTTC	5 cut(s)	2914, 3146, 4288, 4335, 7260
AspLEI	GCGC	5 cut(s)	1434, 1462, 2118, 3825, 4083
AsuNHI	GCTAGC	1 cut(s)	5525
AvaI	CYCGRG	2 cut(s)	1443, 3547
BaeI	ACNNNNGTAYC	2 cut(s)	6928, 6961
BalI	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
BamHI	GGATCC	1 cut(s)	5503
BanII	GRGCYC	2 cut(s)	5168, 6466
BarI	GAAGNNNNNNTAC	6 cut(s)	614, 646, 1659, 1691, 5089, 5121
BbsI	GAAGAC	7 cut(s)	1120, 2095, 3292, 3490, 5097, 5190, 5899
Bbv12I	GWGCWC	8 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6581, 7229
BbvCI	CCTCAGC	1 cut(s)	4689
BceAI	ACGGC	1 cut(s)	6223
BcgI	CGANNNNNNTGC	2 cut(s)	4475, 4509
BciVI	GTATCC	3 cut(s)	1000, 5421, 6909
BclI	TGATCA	1 cut(s)	3529
BfmI	CTRYAG	5 cut(s)	2167, 4760, 4793, 5218, 6999
BfoI	RGCGCY	2 cut(s)	1463, 2119
BfuAI	ACCTGC	3 cut(s)	2322, 4173, 5295
BfuI	GTATCC	3 cut(s)	1000, 5421, 6909
BglII	AGATCT	2 cut(s)	902, 2418
BmcAI	AGTACT	4 cut(s)	1372, 2776, 3440, 3818
BmeT110I	CYCGRG	2 cut(s)	1443, 3547
BmgBI	CACGTC	1 cut(s)	5869
BmrI	ACTGGG	1 cut(s)	5353
BmtI	GCTAGC	1 cut(s)	5529
BmuI	ACTGGG	1 cut(s)	5353
BpiI	GAAGAC	7 cut(s)	1120, 2095, 3292, 3490, 5097, 5190, 5899
BpmI	CTGGAG	8 cut(s)	85, 1033, 1615, 5287, 5472, 5640, 6090, 6338
Bpu10I	CCTNAGC	2 cut(s)	4689, 5508
BpuEI	CTTGAG	4 cut(s)	2194, 3185, 3347, 6621
BsaAI	YACGTR	2 cut(s)	5096, 6018
BsaBI	GATNNNNATC	3 cut(s)	2229, 5033, 6624
BsaHI	GRCGYC	1 cut(s)	6609
BsaI	GGTCTC	7 cut(s)	716, 1385, 1691, 3600, 3893, 6799, 7181
BsaWI	WCCGGW	3 cut(s)	948, 985, 5975
BsaXI	ACNNNNNCTCC	4 cut(s)	3131, 3161, 4270, 4300
Bse3DI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3231, 3236, 4384, 6427, 6501
Bse8I	GATNNNNATC	3 cut(s)	2229, 5033, 6624
BseAI	TCCGGA	1 cut(s)	948
BseJI	GATNNNNATC	3 cut(s)	2229, 5033, 6624
BseMI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3231, 3236, 4384, 6427, 6501
BseMII	CTCAG	8 cut(s)	276, 1332, 1800, 1954, 2976, 4131, 4242, 4680
BseRI	GAGGAG	5 cut(s)	827, 1870, 2060, 2335, 4157
BseYI	CCCAGC	1 cut(s)	2186
BsgI	GTGCAG	6 cut(s)	44, 1683, 3719, 4098, 5076, 5276
Bsh1236I	CGCG	1 cut(s)	4083
BsiHKAI	GWGCWC	8 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6581, 7229
BsiHKCI	CYCGRG	2 cut(s)	1443, 3547
BsiSI	CCGG	3 cut(s)	949, 986, 5976
BslFI	GGGAC	5 cut(s)	1440, 4810, 5356, 5777, 6733
BsmFI	GGGAC	5 cut(s)	1440, 4810, 5356, 5777, 6733
BsmI	GAATGC	5 cut(s)	877, 1079, 3217, 3480, 4836
Bso31I	GGTCTC	7 cut(s)	716, 1385, 1691, 3600, 3893, 6799, 7181
BsoBI	CYCGRG	2 cut(s)	1443, 3547
Bsp1286I	GDGCHC	9 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6466, 6581, 7229
Bsp13I	TCCGGA	1 cut(s)	948
Bsp1407I	TGTACA	3 cut(s)	5539, 6278, 6877
Bsp19I	CCATGG	3 cut(s)	1759, 4905, 7098
BspCNI	CTCAG	8 cut(s)	277, 1331, 1799, 1953, 2977, 4132, 4241, 4681
BspEI	TCCGGA	1 cut(s)	948
BspFNI	CGCG	1 cut(s)	4083
BspMAI	CTGCAG	3 cut(s)	4764, 4797, 7003
BspMI	ACCTGC	3 cut(s)	2322, 4173, 5295
BspOI	GCTAGC	1 cut(s)	5529
BspPI	GGATC	9 cut(s)	1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864, 7336
BspQI	GCTCTTC	1 cut(s)	2649
BspTNI	GGTCTC	7 cut(s)	716, 1385, 1691, 3600, 3893, 6799, 7181
BsrDI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3231, 3236, 4384, 6427, 6501
BsrGI	TGTACA	3 cut(s)	5539, 6278, 6877
BssNAI	GTATAC	1 cut(s)	313
BssNI	GRCGYC	1 cut(s)	6609
BssT1I	CCWWGG	9 cut(s)	993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
Bst1107I	GTATAC	1 cut(s)	313
Bst6I	CTCTTC	9 cut(s)	243, 719, 2070, 2649, 2739, 3341, 4255, 6017, 7332
BstACI	GRCGYC	1 cut(s)	6609
BstAPI	GCANNNNNTGC	5 cut(s)	3200, 3242, 3441, 5696, 6086
BstAUI	TGTACA	3 cut(s)	5539, 6278, 6877
BstBAI	YACGTR	2 cut(s)	5096, 6018
BstDSI	CCRYGG	3 cut(s)	1759, 4905, 7098
BstEII	GGTNACC	1 cut(s)	6488
BstENI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4401, 7273
BstFNI	CGCG	1 cut(s)	4083
BstH2I	RGCGCY	2 cut(s)	1463, 2119
BstHHI	GCGC	5 cut(s)	1434, 1462, 2118, 3825, 4083
BstNSI	RCATGY	6 cut(s)	2730, 3634, 5540, 6068, 6332, 7387
BstPI	GGTNACC	1 cut(s)	6488
BstSFI	CTRYAG	5 cut(s)	2167, 4760, 4793, 5218, 6999
BstUI	CGCG	1 cut(s)	4083
BstV2I	GAAGAC	7 cut(s)	1120, 2095, 3292, 3490, 5097, 5190, 5899
BstX2I	RGATCY	7 cut(s)	902, 2418, 3682, 4806, 5503, 6856, 7341
BstXI	CCANNNNNNTGG	4 cut(s)	1639, 4427, 5045, 6068
BstYI	RGATCY	7 cut(s)	902, 2418, 3682, 4806, 5503, 6856, 7341
BstZ17I	GTATAC	1 cut(s)	313
BsuI	GTATCC	3 cut(s)	1000, 5421, 6909
BtgI	CCRYGG	3 cut(s)	1759, 4905, 7098
BtgZI	GCGATG	1 cut(s)	5527
BtrI	CACGTC	1 cut(s)	5869
BtsI	GCAGTG	3 cut(s)	6119, 6490, 6996
BveI	ACCTGC	3 cut(s)	2322, 4173, 5295
CaiI	CAGNNNCTG	4 cut(s)	3432, 4235, 5663, 6956
CfoI	GCGC	5 cut(s)	1434, 1462, 2118, 3825, 4083
CsiI	ACCWGGT	1 cut(s)	1486
CspCI	CAANNNNNGTGG	2 cut(s)	7060, 7095
DraIII	CACNNNGTG	2 cut(s)	4943, 6086
EaeI	YGGCCR	7 cut(s)	2921, 3287, 5414, 5820, 7101, 7204, 7284
Eam1104I	CTCTTC	9 cut(s)	243, 719, 2070, 2649, 2739, 3341, 4255, 6017, 7332
EarI	CTCTTC	9 cut(s)	243, 719, 2070, 2649, 2739, 3341, 4255, 6017, 7332
Ecl136II	GAGCTC	1 cut(s)	5166
Eco130I	CCWWGG	9 cut(s)	993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
Eco147I	AGGCCT	2 cut(s)	610, 4269
Eco24I	GRGCYC	2 cut(s)	5168, 6466
Eco31I	GGTCTC	7 cut(s)	716, 1385, 1691, 3600, 3893, 6799, 7181
Eco32I	GATATC	5 cut(s)	3576, 4049, 4672, 6218, 6541
Eco47III	AGCGCT	1 cut(s)	2117
Eco53kI	GAGCTC	1 cut(s)	5166
Eco88I	CYCGRG	2 cut(s)	1443, 3547
Eco91I	GGTNACC	1 cut(s)	6488
EcoICRI	GAGCTC	1 cut(s)	5166
EcoNI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4401, 7273
EcoO109I	RGGNCCY	2 cut(s)	5284, 7094
EcoO65I	GGTNACC	1 cut(s)	6488
EcoRI	GAATTC	1 cut(s)	6147
EcoRV	GATATC	5 cut(s)	3576, 4049, 4672, 6218, 6541
EcoT14I	CCWWGG	9 cut(s)	993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
EcoT22I	ATGCAT	2 cut(s)	1081, 2446
EcoT38I	GRGCYC	2 cut(s)	5168, 6466
ErhI	CCWWGG	9 cut(s)	993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
FaqI	GGGAC	5 cut(s)	1440, 4810, 5356, 5777, 6733
FauI	CCCGC	1 cut(s)	6065
FauNDI	CATATG	5 cut(s)	976, 1057, 1214, 4820, 6045
FbaI	TGATCA	1 cut(s)	3529
FblI	GTMKAC	1 cut(s)	312
FriOI	GRGCYC	2 cut(s)	5168, 6466
FspI	TGCGCA	2 cut(s)	1433, 3824
GlaI	GCGC	5 cut(s)	1433, 1461, 2117, 3824, 4082
GsaI	CCCAGC	1 cut(s)	2190
GsuI	CTGGAG	8 cut(s)	85, 1033, 1615, 5287, 5472, 5640, 6090, 6338
HaeII	RGCGCY	2 cut(s)	1463, 2119
HapII	CCGG	3 cut(s)	949, 986, 5976
HhaI	GCGC	5 cut(s)	1434, 1462, 2118, 3825, 4083
Hin1I	GRCGYC	1 cut(s)	6609
Hin6I	GCGC	5 cut(s)	1432, 1460, 2116, 3823, 4081
HinP1I	GCGC	5 cut(s)	1432, 1460, 2116, 3823, 4081
HincII	GTYRAC	7 cut(s)	933, 1549, 3414, 4630, 6564, 6613, 7072
HindII	GTYRAC	7 cut(s)	933, 1549, 3414, 4630, 6564, 6613, 7072
HindIII	AAGCTT	8 cut(s)	757, 1702, 2250, 2517, 3196, 5329, 6334, 6987
HpaI	GTTAAC	2 cut(s)	1549, 4630
HpaII	CCGG	3 cut(s)	949, 986, 5976
HpyCH4IV	ACGT	9 cut(s)	1545, 3388, 3560, 3583, 4491, 5095, 5868, 6017, 6609
HpySE526I	ACGT	9 cut(s)	1545, 3388, 3560, 3583, 4491, 5095, 5868, 6017, 6609
Hsp92I	GRCGYC	1 cut(s)	6609
HspAI	GCGC	5 cut(s)	1432, 1460, 2116, 3823, 4081
Kpn2I	TCCGGA	1 cut(s)	948
Ksp22I	TGATCA	1 cut(s)	3529
KspAI	GTTAAC	2 cut(s)	1549, 4630
LguI	GCTCTTC	1 cut(s)	2649
MabI	ACCWGGT	1 cut(s)	1486
MaeII	ACGT	9 cut(s)	1545, 3388, 3560, 3583, 4491, 5095, 5868, 6017, 6609
MfeI	CAATTG	2 cut(s)	5462, 6754
MflI	RGATCY	7 cut(s)	902, 2418, 3682, 4806, 5503, 6856, 7341
MhlI	GDGCHC	9 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6466, 6581, 7229
MlsI	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
MluNI	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
MlyI	GAGTC	7 cut(s)	267, 296, 2237, 4241, 5378, 5456, 5566
MmeI	TCCRAC	2 cut(s)	478, 2739
Mox20I	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
Mph1103I	ATGCAT	2 cut(s)	1081, 2446
MroI	TCCGGA	1 cut(s)	948
MroXI	GAANNNNTTC	5 cut(s)	2914, 3146, 4288, 4335, 7260
MscI	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
Msp20I	TGGCCA	6 cut(s)	2923, 3289, 5416, 5822, 7103, 7286
MspA1I	CMGCKG	3 cut(s)	50, 2754, 5663
MspI	CCGG	3 cut(s)	949, 986, 5976
MunI	CAATTG	2 cut(s)	5462, 6754
Mva1269I	GAATGC	5 cut(s)	877, 1079, 3217, 3480, 4836
MvnI	CGCG	1 cut(s)	4083
NcoI	CCATGG	3 cut(s)	1759, 4905, 7098
NdeI	CATATG	5 cut(s)	976, 1057, 1214, 4820, 6045
NheI	GCTAGC	1 cut(s)	5525
NmuCI	GTSAC	7 cut(s)	4244, 4814, 5855, 6946, 6994, 7049, 7145
NsbI	TGCGCA	2 cut(s)	1433, 3824
NsiI	ATGCAT	2 cut(s)	1081, 2446
NspI	RCATGY	6 cut(s)	2730, 3634, 5540, 6068, 6332, 7387
PaeI	GCATGC	1 cut(s)	2730
PceI	AGGCCT	2 cut(s)	610, 4269
PciSI	GCTCTTC	1 cut(s)	2649
PcsI	WCGNNNNNNNCGW	1 cut(s)	6226
PctI	GAATGC	5 cut(s)	877, 1079, 3217, 3480, 4836
PdmI	GAANNNNTTC	5 cut(s)	2914, 3146, 4288, 4335, 7260
PflMI	CCANNNNNTGG	2 cut(s)	3679, 5423
PfoI	TCCNGGA	1 cut(s)	2617
PleI	GAGTC	7 cut(s)	267, 295, 2237, 4241, 5377, 5455, 5565
PpsI	GAGTC	7 cut(s)	267, 295, 2237, 4241, 5377, 5455, 5565
Ppu21I	YACGTR	2 cut(s)	5096, 6018
PpuMI	RGGWCCY	2 cut(s)	5284, 7094
PshBI	ATTAAT	1 cut(s)	6204
PsiI	TTATAA	3 cut(s)	3927, 5843, 7013
Psp124BI	GAGCTC	1 cut(s)	5168
Psp1406I	AACGTT	1 cut(s)	1545
Psp5II	RGGWCCY	2 cut(s)	5284, 7094
PspEI	GGTNACC	1 cut(s)	6488
PspFI	CCCAGC	1 cut(s)	2186
PspPPI	RGGWCCY	2 cut(s)	5284, 7094
PstI	CTGCAG	3 cut(s)	4764, 4797, 7003
PstNI	CAGNNNCTG	4 cut(s)	3432, 4235, 5663, 6956
PsuI	RGATCY	7 cut(s)	902, 2418, 3682, 4806, 5503, 6856, 7341
PvuII	CAGCTG	2 cut(s)	50, 5663
SacI	GAGCTC	1 cut(s)	5168
SapI	GCTCTTC	1 cut(s)	2649
ScaI	AGTACT	4 cut(s)	1372, 2776, 3440, 3818
SchI	GAGTC	7 cut(s)	267, 296, 2237, 4241, 5378, 5456, 5566
SduI	GDGCHC	9 cut(s)	1154, 2848, 4683, 5168, 5411, 6235, 6466, 6581, 7229
SexAI	ACCWGGT	1 cut(s)	1486
SfcI	CTRYAG	5 cut(s)	2167, 4760, 4793, 5218, 6999
SmlI	CTYRAG	4 cut(s)	2173, 3164, 3326, 6636
SmoI	CTYRAG	4 cut(s)	2173, 3164, 3326, 6636
SphI	GCATGC	1 cut(s)	2730
SseBI	AGGCCT	2 cut(s)	610, 4269
SspI	AATATT	7 cut(s)	1467, 2269, 2638, 2839, 3008, 3294, 4654
SstI	GAGCTC	1 cut(s)	5168
StuI	AGGCCT	2 cut(s)	610, 4269
StyI	CCWWGG	9 cut(s)	993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
TaiI	ACGT	9 cut(s)	1548, 3391, 3563, 3586, 4494, 5098, 5871, 6020, 6612
TaqI	TCGA	7 cut(s)	231, 1105, 3472, 4045, 5560, 5947, 6220
TaqII	GACCGA	1 cut(s)	758
TauI	GCSGC	3 cut(s)	2906, 7129, 7209
TseFI	GTSAC	7 cut(s)	4244, 4814, 5855, 6946, 6994, 7049, 7145
Tsp45I	GTSAC	7 cut(s)	4244, 4814, 5855, 6946, 6994, 7049, 7145
TspGWI	ACGGA	6 cut(s)	869, 1085, 4565, 4802, 6074, 7089
Van91I	CCANNNNNTGG	2 cut(s)	3679, 5423
VspI	ATTAAT	1 cut(s)	6204
XagI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4401, 7273
XapI	RAATTY	7 cut(s)	2366, 2663, 3128, 3142, 4444, 6147, 6192
XbaI	TCTAGA	3 cut(s)	808, 899, 2686
XceI	RCATGY	6 cut(s)	2730, 3634, 5540, 6068, 6332, 7387
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4912
XmiI	GTMKAC	1 cut(s)	312
XmnI	GAANNNNTTC	5 cut(s)	2914, 3146, 4288, 4335, 7260
ZraI	GACGTC	1 cut(s)	6610
ZrmI	AGTACT	4 cut(s)	1372, 2776, 3440, 3818
Zsp2I	ATGCAT	2 cut(s)	1081, 2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.