Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006607

Physical Location & Seq

Genomic Coordinates Forward (+)

41193524 .. 41207578

Gene Structure 14055 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1292400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7440 bp

ATGGGGAGACTTCTTGGTGGTTTCGTGTTGCCTCTGCTGCTGCTAACTGCTGGTGTTATCAACTGGAGCGTGCTCTCTCTTGTCGATTTGATAGCGTTTCTTCTTATTCTATTTAATGCACCAAAACTAGGATTTCATCTCGGAAGGCAGCTTTTATTGTCATGGATCATTGTTATATTTTCTTTATTTGTTATTTTTTCTCAAGTGATATATCTTGTTATATGGGCTATTGAGGGAAATAAATGGAGCGGAGTAGATGCTTGGTGGGCAAATCTTATTGGATTCATGATATTGCAGTCATGGAAATCTCCTTTCGTGCTATATTTTTTACTCTTACAACTATCAGTAGTTGCTGTTGCATTAGTTGATTTGTATGGAAACAGATTTGGTCTTGTTTCATCATGCGATTCATGTTGGGGTCGTTTCTCATCAGCCGTTGAACGATTAATTTGTTCTCATCTTAGGGTTGCTGTCTTGTTGCTGTTGCCTGCCATTCAGTTGGTTGTCGGAATTAGCCATCCTTCATGGGTTTCTCTACCCTTTTTTATTGGGAGCTGCATTGGTCTTGTAGATTGGTCTTTGACAAGCAATTTTCTAGGACTTTTCAGGTGGTGGAAGCCTTTTCACTTGTATGCAGGCTTCAACATTGTCTTGCTTTATGTGTATCAGCTCCCTGTGGAGTTTCCGGATATGCTACAATGGGTAGCTAAGTTCATTGGTCTGTTCAAAATAACTTTACATTCTGACTGGACTGAAGTTTGTTCGAGCTGCTCTCTTTTACTTTTCTACACCATGCTATCCTATGTTAAATGTGATCTAGAGGAAATGGATTGTATTTTATCCATGAAAGAAAACAACTTGATGGAGCAACTTCTTCCCTCGAAGCATTCATTTTTTATTCGTGAATCTAGATCTGGTGTGAGGCATACAAATGTTTTATTGACGGGAGCGGTTTTCCGGACCTTTAGTATCAACTTCTTCACATATGGTTTCCCGGTTTCCTTGTTTGCTCTTTCTTTTTGGAGTTTTCACTTTGCAAGCATTTGCGCATTTGGGCTACTTGCATATGTTGGCTACATCATATATGTCTTCCCTTCCTTATTCCGTTTGCATAGACTGAATGGGCTGCTTCTTGTCTTCATTCTCCTGTGGGCTGCTAGCACATATATTTTCAATGTAGCATTTGCATTCTTGAATCGGAAAATCGGAAAGAACATGGACATATGGGAGATGATTGGGTTGTGGCATTATCCCATACCTGGATTCTTTCTGCTTGCACAATTTTGTCTTGGAATATTGGTTGCCTTGGGTAATCTTGTGAACAACTCGGTTTTCCTTTGGTTGTCTGATGAGGATGGACAATCTTCGAATGACAACAGTACTGCAGAAGGCGAGGGAGAGACCAAGGTATTGATAGTGGCCACAATTGCTTGGGGACTGCGCAAAAGCTCTCGAGCTATCATGCTGGCACTGATATTGCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTATATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACATTAGCAGAAAGATGCGCAAGTCTTTGATTCTCCTATGTGAGGTTCACTTTGCGCTATTGTATATCATTCAGATTAATCCGATCTCTGATACTTTGGAGCAAAAAGGCTCTTTGAGTGCAGAAATCTTATCACAATTAGGTCTTGTTCAACATGAAAGCTCTTGGGACTTTTTGCAAATAGCTTTGCTTGCTTGCTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTATTTTCGTTCTCAGCAATTGTGCAGCATACACCTAGCCGTCCATTTGGATTTAGCATTTTGAAAGCTGGTCTGAATAAATCAGTTTTGTTGTCAGTGTATGCGTCCTCAGCCATTCAGTACAGACATGATAATCCTTCTTATGAGAGAAGAATCGCATTATTCCTCAGTGCAATTGGGCAGAAGTTTTTATCTGTGTACAGATCATGTGGAACCTACATTGCCTTTCTAACGATTCTCCTTACCGTATACTTGGTGAGGCCCAATTATGTATCATTTGGATACATATTCCTTCTCCTTGTTTGGATCATTGGAAGACAACTTGTCGAGAGAACAAAAAAACGCCTTTGGTTCCCATTGAAAGCATATGCAATTATGGTGTTTATCTTTATGTATAGCTTGAGCAGTTTCCTGAGCATTGAGATTTGGTTGTCCAAGTTCATAGACCTTTATTTTTATCTAGGCTATAACTCAGAAGCTTCATGTTTGAAAAATATTTGGGAATCCCTAGCAGTGTTGATTGTGATGCAACTTTATAGCTACGAGAGAAGGCAGAGCAGTTATAACAGGTCAGATGATGTCCATGTGTTAGAATTTGGAGTGCTTGGGTTTGTAAGGCGGTTTGTCATTTGGCACAGCAACAAGATCTTGTTTATTGCAGTATTCTATGCATCTTTATCTCCAATAAGTGCCTTTGGCTTCTTTTATCTACTTGGCCTTGTCCTGTGTTCAACTTTACCTAAAGCTTCACATTTCCCATCTAAATCATTCTTGGTTTACACAGGATTCCTAGTAACGTCTGAGTACCTTTTTCAGATGTGGGGTAGACAGGCTGCAATGTTTCCTGGACAAAAGCAGTCTTATATTTCCCTTTTTTTGGGTTTCCGTGTATTTAAACCAGGCTTTTGGGGTCTAGAATCTGGCCTGAGAGGAAAAGTTCTGGTGATTGCTGCATGCACTCTTCAATATAATGTTTTTCGCTGGTTGAAAAAGATGCCAAGTACTATCCTAAACAATGGAAAGTGGGAAGAGCCTTGTCCATTGTTTGTCTCAACAGAAGATGCCAATATCAATGGTTCCATCCCAAGGGAGGATAACAAGCCATCGACAGATTCTGAAGCCATTTCAGTGAAACGAGAAGGGGTTAGAAGCCAATCATGGCCCTTTTTTTCCCCTGGTTCATCGCAATCACCTAACCATGTGTCCCCTAGAGCAGGAGGTTCTGAGGGTAGCAGTAATAAATATTCATTTGGGTACATTTGGGGCAGCACCAAAGAGAGTCATAAGTGGAACAAGAAGCGGATTCTTGCCTTGAGAAAGGAGAGATTTGACACACAGAAGCTTATCGCTAAAATCTATTTGAAATTCTGGATGGAGAATATGTTTAACCTCTTTGGTCTTGAGATAAACATGATTGCATTGCTTCTTGCAAGCTTTGCTTTATTGAATGCCATTTCTATGGTATACATTGCGTTACTTGCTACTTGCATTATTTTGAATCGGCAGAGTATCCGTAAACTATGGCGCATATTAGTTTTCCTGTTTGCATCCATTCTCATCCTTGAGTATTTTGCCATCTGGAGGAGTATGTGGACTCGAAATCAACCTGATGAGATGATTGCACAGTGCCATGATTGCTGGAAAAGCTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTATTGTCGATGATCCGCGTATGCTTATCAGCTATTTTGCCGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCAATTGTCCGGATTCTCGGTGTCATCTACTTACCGACAAATGGTATCTCAACGTAAAAATTTGTTTGTGTGGAGAGACCTCTCTTTTGAAACCAAAAGCATGTGGACCTTCTTTGACTACCTGAGGCTTTACTGCTACTGTCATCTGTTGGATCTTGTGCTAGCATTGGTTTTGATTACTGGAACAATTGAGTATGACATTCTGCACCTTGGTTATCTTGCTTTTGCACTGGTTTTCTTTCGGGTGAGACTTGAAATCTTAAAGAAGAGGAACAAGATATTCAAGTTCTTGCGCATATACAATTTTGCGCTTATTGTTCTTTCTCTTGCATATCAATCTCCTTTTGTGGGGGAGTCTTGTGCTGGAAAGTGTGAGACAGTAGATTACGTATTTGAGATGATTGGATTTTATAAGTATGACTATGGATTTCGGATTACTGCAAGATCGGCTCTTGTTGAGATTGTCATTTTTATGCTGGTATCACTTCAGTCCTATATGTTCTCCTCCCCAGAGTTTGATTATGTTTCTCGATATCTTGAAGCCGAGCAAATTGGTGCCATTGTGCGTGAGCAAGAAAAGAAAGCTGCATGGAAAACTGCACAGTTAAAACATATTCGTGAATCTGAGGAGAAGAAACACCAGCGTAACCTGCAAGTGGAGAAAATGAAGTCAGAGATGCTCAACCTGCAAACCCAACTCCACAGCACAAACTCGGTTACTGATTGCGGTGACTCTCCTCCTGTCAGTGAAGGCCTGAGAAGGAGGAGGAATATTTCTCTTAACTTGAACAACGATTGGGGGACCTCTGATAAAGAAGGATTACAACTGAAAAAGGAGCAGATACTTAGAGAGGATTCATTATATCCTTGTGAATTGCATGAATCTCCTGCACCAGTGAATATGGAAAATCCAACAGGGATGGAGTGTGCGAGAGATTCAATAGAATCTCTGAACAGTGAGATCACTGAAGTTGAAGATGATGTTGCTGATGGCGTATTTTTCACTTCTTCAGAAAAGGACAAAGTTAAAGGGAAAGGGAAGGAAAGCCCACTAATATCTCTGCTAGGTGATGGTGTTTCCCAGGTACAGTCGATTGGAAATCAGGCAGTTAACAACCTTGTAAGCTTTTTGAACATTGATCATGAGTTTGATGTCAATGAGCACTCTTCTGTTGAGGATGGGGTGTATGATGAGATGGAGAGCCAGAAAGTTAAAGTGTCCTTTGACCGTTCATCTTCCCTGCAGTCTGACACGAGTTCTGATGCAACAAGTCTGCAGTTAGGAAGGATCCTCCGTCACATATGGTCCCAAATGCGATCCAACAATGATATTGTGTGTTATTGTTGTTTTATCCTTGTCTTTTTGTGGAACTTCAGTTTGCTTTCTATGGTGTATCTGGCGGCTTTATTCTTGTACGCACTATGTGTAAATTCCCGTCCAAGTTACATCTTCTGGGTTGTTATGCTGATTTACACAGAAATCTATATTTTGCTTCAGTATCTGTACCAGATTATCATCCAGCACTGGGCTTTTAGTGTTACTTCAGACTTGCTTCGCGAGTGGGGATTTCCTGAACATAAAATCACATCTTCTTTTGTGGTCAGTTCATTACCTCTCTTTCTTGTCTACGTATTTACCCTCTTACAGAGCTCTATAACCGCAAAAGATGGTGAGTGGATGTCGTCTACAGACTTCGACTTCTATAGGAGGAGTGCTTTGCATGGAAAAGATATTCCTATCAGCTATAGCTGGAGTCAGAAAACAAAGAAGTTTCTGCAAATAATGGAAAATGCAATTAAATCGATAATCAGAAGCTTCTTTAGGTACTGGAATTCACTGACACATGGAGCAGATTCCCCTCCTTACTTTGTTCAGGTGTCTATGGATGTGCGCTCATGGCCAGAGGACGGGATTCAGCCAGAAAAGATTGAGTCTGGAGTAAATCAATTGCTTAAAATCATTCATGATGAAAGGTGCAAGGAGAAAAACCCTAATCTCTGCCCTTTCGCTAGCAGGGTGCATGTCCAAAGCATTGAAAGGAGTCAAGAAAATGAAAATGTAGCCTTGGCTGTTTTTGAGGTGGTATATGCCTCTCCCATGACAGAGTGTGGTGATTCGATAGAATGGTACAAGTCACTTACTCCAGCAGCTGATGTTGCAAAGGAAATTCTTAAAGCACAGGATGCTGGGTTTGTTGAAGAAATAGGATTCCCATACCCTATACTCTCTGTAATTGGAGGAGGCAAAAGGGACATTGATGTGTATGCATATGTATTTGGTGCCGATCTGACTGTTTTCTTTTTAGTCGCCATATTCTACCAATCCGTAAGAAAAAATAACAGCGAATTTCTTGACGTGTATCAGCTTGAAGATCAGTTTCCGAAAGAGTTTGTATTCATCTTAATGATTATCTTTTTCTTGATTGTGCTTGACCGTATAATCTATCTCTGTTCATTTGCCTCCGGAAAAGTGATTTTCTACCTTTTCAACCTCATCCTCTTCACATATTCAGTAACAGAGTATGCTTGGAACATGGAGCCTTCCCACCATGCTGGAAGATTAGCACTCCGTGCTATATTTCTTGCGAAAGCAGTTTCTTTAGCACTGCAGGCTATCCAACTCCGACATGGAATTCCTCATAAAGGCACTTTGTACCGGCAGTTTTTGACTAGTGAGATTTCAAGAATTAATTATTTGGGATATCGACTATATCGCGCTCTGCCTTTCCTTTATGAACTGAGATGTGCACTTGATTGGTCATGCACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTTGTCAAATGTGATGCAGTCTTGAATAGAGCAACGCACAAACAAGGAGAAAAGCAAACGAAAATGACCAAATGTTGCAACGGGATATGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTACAGCAGTGGTAACCCGACGAACATTGAAAATCCCATCAAAGATGCCAGTGTTCAGGTTGACATTAAGACAGCGAGTGGAAGATTGACCCTGTATCAAACCACCCTCTGTGAAAAGCTCCAATGGGACAAACTTGACTCTGATGTTAATCTTGACCCTGAAGGTTACTTGGATACCTATAATAAGAAAGATGTCCAGTTGATATGTTGCGAATCTGATGCTAGTACTTTGTGGCTTATTCCCGATGTAGTTCAAACCAGATTCATTCGGTCCCTTGATTGGGATCCCAACATGGATATATCTTTCACTTGGGTACTTTCCAGGGACAGGCCCAAAGGCAAGGAAACTGTGAAATATGAAAGAAGTTTGGAGTCTCAGGATCTTCCGAAACAATCAGATGTCCAAAAAGTTCTCAACGGCTCCATTAACAGCTTTAGGATGTACAACATTTATCCAAGATACTTCCGTGCTGGTTCTGGTGATGTCAGACCGTTGGAACTAGAGGATAATTTTGTTAACGCGGACCTTGTTTTGAATCGTGCAAATTATGAGTGGTGGTCCTTCCGTGACACCAATTCATCAGATATCAATGGATGTGGAGGTTTGACAGGACCGATGGCCATCATCGTATCTGAGGAAACACCGCCACAGGGTATTCTTGGTGACACCCTTAGCAAGTTCAGCATTTGGGGTCTCTACTTAACATTTGTTCTTGCCGTTGGCCGCTTCATCAGACTTCAATGTTCCGACTTACGAATGAGAATCCCCTATGAGAACCTTCCTTCCTGTGATAGGTTGATAGCCATTTGTGAGGACATATATGCTGCAAGAGCAGAGGGTGAGCTTGGAGTTGAAGAGGTCTTATACTGGACACTGGTGAAGATTTACCGGTCACCACACATGCTGCTCGAATACACGAAACCGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0015075 GO:0015267 GO:0015318 GO:0016020 GO:0022803 GO:0022836 GO:0022838 GO:0022857 GO:0022890 GO:0034220 GO:0042391 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0065007 GO:0065008 GO:0071214 GO:0071260 GO:0071496 GO:0098655 GO:0104004

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Piezo_RRas_bdg

Protein Analysis

2480

Amino Acids

284.76

Weight (kDa)

7.74

Isoelectric Point (pI)

46.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIEZO	PF25288	472 - 601	1.4e-63	Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1	PF23188	1623 - 1742	2.3e-18	Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor	PF24874	1936 - 2172	3.2e-67	Piezo, THU9 and anchor domain
Piezo_cap	PF12166	2200 - 2478	1.5e-78	Piezo non-specific cation channel, cap domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006607)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48060 AT2G48060 AT2G48060
fragaria_vesca	FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180
malus_domestica	MD03G1271800.v1.1 MD11G1292400.v1.1
prunus_persica	Prupe.8G248000_v2.0.a1 Prupe.8G248000_v2.0.a1
pyrus_communis	pycom03g21640 pycom11g25880
rosa_chinensis	RchiOBHm_Chr6g0291991
rosa_laevigata	RLG00000012072
rosa_multiflora	Rmu_sc0008666.1_g000011
rosa_roxburghii	Rroxscaffold_7G00175510 Rroxscaffold_7G00178010
rosa_rugosa	Rorug06G0222200 Rorug06G0222300
rosa_samantha	Rh6AG336100 Rh6BG343500 Rh6CG349700 Rh6DG336500
rosa_wichuraiana	Rw6G029260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2346, 3960
Acc16I	TGCGCA	4 cut(s)	1048, 1442, 1571, 3842
Acc36I	ACCTGC	2 cut(s)	4206, 4242
AccB1I	GGYRCC	2 cut(s)	4103, 5828
AccBSI	CCGCTC	2 cut(s)	249, 950
AccI	GTMKAC	5 cut(s)	2031, 2608, 3246, 5175, 5234
AccII	CGCG	4 cut(s)	3490, 5106, 6264, 7031
AccIII	TCCGGA	4 cut(s)	685, 957, 3557, 6011
AcoI	YGGCCR	4 cut(s)	1419, 5447, 7128, 7231
AcsI	RAATTY	8 cut(s)	2375, 3146, 3607, 4978, 5380, 5715, 5894, 6180
AcuI	CTGAAG	9 cut(s)	774, 2919, 4019, 4536, 4542, 4906, 5027, 5076, 6690
AdeI	CACNNNGTG	2 cut(s)	4973, 6119
AflIII	ACRYGT	1 cut(s)	5904
AgeI	ACCGGT	1 cut(s)	7398
AhdI	GACNNNNNGTC	2 cut(s)	2105, 3476
AhlI	ACTAGT	1 cut(s)	6218
AjiI	CACGTC	1 cut(s)	5905
AjnI	CCWGG	7 cut(s)	1258, 1495, 2626, 2680, 2956, 4629, 6830
AloI	GAACNNNNNNTCC	2 cut(s)	7277, 7309
Alw21I	GWGCWC	4 cut(s)	75, 4713, 5201, 6298
Alw26I	GTCTC	7 cut(s)	1394, 2836, 3618, 3790, 3917, 6888, 7208
Alw44I	GTGCAC	1 cut(s)	6294
AlwNI	CAGNNNCTG	4 cut(s)	2897, 3450, 5050, 5699
Ama87I	CYCGRG	1 cut(s)	1452
Aor13HI	TCCGGA	4 cut(s)	685, 957, 3557, 6011
ApaLI	GTGCAC	1 cut(s)	6294
ApoI	RAATTY	8 cut(s)	2375, 3146, 3607, 4978, 5380, 5715, 5894, 6180
ArsI	GACNNNNNNTTYG	2 cut(s)	3617, 3649
AseI	ATTAAT	3 cut(s)	446, 1629, 6237
AsiGI	ACCGGT	1 cut(s)	7398
AspLEI	GCGC	9 cut(s)	1049, 1443, 1572, 1609, 3309, 3843, 3859, 5442, 6266
AsuC2I	CCSGG	1 cut(s)	995
AsuII	TTCGAA	1 cut(s)	1367
AsuNHI	GCTAGC	3 cut(s)	1157, 3709, 5558
AvaI	CYCGRG	1 cut(s)	1452
AvaII	GGWCC	8 cut(s)	960, 3654, 4350, 4854, 6780, 7033, 7068, 7121
AxyI	CCTNAGG	1 cut(s)	3671
BaeGI	GKGCMC	1 cut(s)	6298
BaeI	ACNNNNGTAYC	2 cut(s)	6961, 6994
BalI	TGGCCA	3 cut(s)	1421, 5449, 7130
BamHI	GGATCC	2 cut(s)	4836, 6793
BanI	GGYRCC	2 cut(s)	4103, 5828
BanII	GRGCYC	2 cut(s)	5201, 6499
BarI	GAAGNNNNNNTAC	2 cut(s)	623, 655
BauI	CACGAG	1 cut(s)	4801
BbsI	GAAGAC	4 cut(s)	1081, 1129, 2104, 3508
Bbv12I	GWGCWC	4 cut(s)	75, 4713, 5201, 6298
BbvCI	CCTCAGC	1 cut(s)	1891
BceAI	ACGGC	5 cut(s)	419, 1806, 3497, 6943, 7211
BcgI	CGANNNNNNTGC	4 cut(s)	5331, 5365, 6710, 6744
BciT130I	CCWGG	7 cut(s)	1260, 1497, 2628, 2682, 2958, 4631, 6832
BciVI	GTATCC	3 cut(s)	2057, 3302, 6676
BclI	TGATCA	2 cut(s)	3547, 4687
BcnI	CCSGG	1 cut(s)	995
BcoDI	GTCTC	7 cut(s)	1394, 2836, 3618, 3790, 3917, 6888, 7208
BcuI	ACTAGT	1 cut(s)	6218
BfmI	CTRYAG	7 cut(s)	1383, 4790, 4823, 5235, 5251, 5293, 6155
BfuAI	ACCTGC	2 cut(s)	4206, 4242
BfuI	GTATCC	3 cut(s)	2057, 3302, 6676
BglII	AGATCT	2 cut(s)	911, 2427
BmcAI	AGTACT	4 cut(s)	1381, 2785, 3458, 6736
Bme1390I	CCNGG	8 cut(s)	995, 1260, 1497, 2628, 2682, 2958, 4631, 6832
Bme18I	GGWCC	8 cut(s)	960, 3654, 4350, 4854, 6780, 7033, 7068, 7121
BmeRI	GACNNNNNGTC	2 cut(s)	2105, 3476
BmeT110I	CYCGRG	1 cut(s)	1452
BmgBI	CACGTC	1 cut(s)	5905
BmrFI	CCNGG	8 cut(s)	995, 1260, 1497, 2628, 2682, 2958, 4631, 6832
BmrI	ACTGGG	1 cut(s)	5083
BmtI	GCTAGC	3 cut(s)	1161, 3713, 5562
BmuI	ACTGGG	1 cut(s)	5083
BpiI	GAAGAC	4 cut(s)	1081, 1129, 2104, 3508
BplI	GAGNNNNNCTC	2 cut(s)	757, 789
BpmI	CTGGAG	6 cut(s)	85, 3382, 5320, 5505, 5676, 6371
Bpu10I	CCTNAGC	3 cut(s)	1891, 2195, 7181
Bpu14I	TTCGAA	1 cut(s)	1367
BpuEI	CTTGAG	5 cut(s)	186, 2203, 3115, 3203, 3365
BpuMI	CCSGG	1 cut(s)	995
Bsa29I	ATCGAT	1 cut(s)	5351
BsaAI	YACGTR	2 cut(s)	3937, 5179
BsaBI	GATNNNNATC	4 cut(s)	5063, 6657, 6792, 7130
BsaI	GGTCTC	3 cut(s)	1394, 3618, 7208
BsaJI	CCNNGG	9 cut(s)	1305, 1404, 1768, 2867, 2956, 3757, 4629, 5613, 6831
BsaWI	WCCGGW	6 cut(s)	685, 957, 3557, 6011, 7398, 7432
BsaXI	ACNNNNNCTCC	2 cut(s)	3149, 3179
Bse118I	RCCGGY	2 cut(s)	6204, 7398
Bse21I	CCTNAGG	1 cut(s)	3671
Bse3DI	GCAATG	5 cut(s)	1482, 2001, 2625, 3200, 3249
Bse8I	GATNNNNATC	4 cut(s)	5063, 6657, 6792, 7130
BseAI	TCCGGA	4 cut(s)	685, 957, 3557, 6011
BseBI	CCWGG	7 cut(s)	1260, 1497, 2628, 2682, 2958, 4631, 6832
BseCI	ATCGAT	1 cut(s)	5351
BseDI	CCNNGG	9 cut(s)	1305, 1404, 1768, 2867, 2956, 3757, 4629, 5613, 6831
BseJI	GATNNNNATC	4 cut(s)	5063, 6657, 6792, 7130
BseMI	GCAATG	5 cut(s)	1482, 2001, 2625, 3200, 3249
BseRI	GAGGAG	7 cut(s)	3379, 4042, 4190, 4275, 4327, 5272, 5802
BseSI	GKGCMC	1 cut(s)	6298
BseYI	CCCAGC	1 cut(s)	5735
BsgI	GTGCAG	5 cut(s)	1692, 1826, 3737, 4131, 4422
Bsh1236I	CGCG	4 cut(s)	3490, 5106, 6264, 7031
BshNI	GGYRCC	2 cut(s)	4103, 5828
BshTI	ACCGGT	1 cut(s)	7398
BshVI	ATCGAT	1 cut(s)	5351
BsiHKAI	GWGCWC	4 cut(s)	75, 4713, 5201, 6298
BsiHKCI	CYCGRG	1 cut(s)	1452
BsiSI	CCGG	8 cut(s)	686, 958, 995, 3558, 6012, 6205, 7399, 7433
BslFI	GGGAC	9 cut(s)	1449, 1733, 2971, 4363, 4840, 5813, 6650, 6766, 6848
BsmAI	GTCTC	7 cut(s)	1394, 2836, 3618, 3790, 3917, 6888, 7208
BsmFI	GGGAC	9 cut(s)	1449, 1733, 2971, 4363, 4840, 5813, 6650, 6766, 6848
BsmI	GAATGC	3 cut(s)	886, 1187, 3235
Bso31I	GGTCTC	3 cut(s)	1394, 3618, 7208
BsoBI	CYCGRG	1 cut(s)	1452
Bsp119I	TTCGAA	1 cut(s)	1367
Bsp1286I	GDGCHC	5 cut(s)	75, 4713, 5201, 6298, 6499
Bsp13I	TCCGGA	4 cut(s)	685, 957, 3557, 6011
Bsp1407I	TGTACA	3 cut(s)	1980, 6510, 6951
Bsp19I	CCATGG	1 cut(s)	1768
Bsp68I	TCGCGA	1 cut(s)	5106
BspDI	ATCGAT	1 cut(s)	5351
BspEI	TCCGGA	4 cut(s)	685, 957, 3557, 6011
BspFNI	CGCG	4 cut(s)	3490, 5106, 6264, 7031
BspHI	TCATGA	3 cut(s)	285, 4690, 5512
BspMAI	CTGCAG	4 cut(s)	1387, 4794, 4827, 6159
BspMI	ACCTGC	2 cut(s)	4206, 4242
BspOI	GCTAGC	3 cut(s)	1161, 3713, 5562
BspQI	GCTCTTC	1 cut(s)	2805
BspT104I	TTCGAA	1 cut(s)	1367
BspT107I	GGYRCC	2 cut(s)	4103, 5828
BspTNI	GGTCTC	3 cut(s)	1394, 3618, 7208
BsrBI	CCGCTC	2 cut(s)	249, 950
BsrDI	GCAATG	5 cut(s)	1482, 2001, 2625, 3200, 3249
BsrFI	RCCGGY	2 cut(s)	6204, 7398
BsrGI	TGTACA	3 cut(s)	1980, 6510, 6951
BssAI	RCCGGY	2 cut(s)	6204, 7398
BssECI	CCNNGG	9 cut(s)	1305, 1404, 1768, 2867, 2956, 3757, 4629, 5613, 6831
BssNAI	GTATAC	2 cut(s)	2032, 3247
BssSI	CACGAG	1 cut(s)	4801
BssT1I	CCWWGG	6 cut(s)	1305, 1404, 1768, 2867, 3757, 5613
Bst1107I	GTATAC	2 cut(s)	2032, 3247
Bst2BI	CACGAG	1 cut(s)	4801
Bst2UI	CCWGG	7 cut(s)	1260, 1497, 2628, 2682, 2958, 4631, 6832
Bst6I	CTCTTC	6 cut(s)	2748, 2805, 3809, 4720, 6053, 7359
BstAPI	GCANNNNNTGC	4 cut(s)	3218, 3459, 5732, 6119
BstAUI	TGTACA	3 cut(s)	1980, 6510, 6951
BstBAI	YACGTR	2 cut(s)	3937, 5179
BstBI	TTCGAA	1 cut(s)	1367
BstDSI	CCRYGG	1 cut(s)	1768
BstEII	GGTNACC	2 cut(s)	6521, 7401
BstENI	CCTNNNNNAGG	4 cut(s)	1592, 2994, 6189, 7300
BstFNI	CGCG	4 cut(s)	3490, 5106, 6264, 7031
BstHHI	GCGC	9 cut(s)	1049, 1443, 1572, 1609, 3309, 3843, 3859, 5442, 6266
BstMAI	GTCTC	7 cut(s)	1394, 2836, 3618, 3790, 3917, 6888, 7208
BstNI	CCWGG	7 cut(s)	1260, 1497, 2628, 2682, 2958, 4631, 6832
BstNSI	RCATGY	5 cut(s)	2739, 3652, 5573, 6365, 7414
BstPI	GGTNACC	2 cut(s)	6521, 7401
BstSCI	CCNGG	8 cut(s)	993, 1258, 1495, 2626, 2680, 2956, 4629, 6830
BstSFI	CTRYAG	7 cut(s)	1383, 4790, 4823, 5235, 5251, 5293, 6155
BstSLI	GKGCMC	1 cut(s)	6298
BstSNI	TACGTA	2 cut(s)	3937, 5179
BstUI	CGCG	4 cut(s)	3490, 5106, 6264, 7031
BstV2I	GAAGAC	4 cut(s)	1081, 1129, 2104, 3508
BstX2I	RGATCY	6 cut(s)	911, 2427, 3700, 4836, 6793, 6889
BstXI	CCANNNNNNTGG	4 cut(s)	499, 2688, 3241, 6101
BstYI	RGATCY	6 cut(s)	911, 2427, 3700, 4836, 6793, 6889
BstZ17I	GTATAC	2 cut(s)	2032, 3247
Bsu15I	ATCGAT	1 cut(s)	5351
Bsu36I	CCTNAGG	1 cut(s)	3671
BsuI	GTATCC	3 cut(s)	2057, 3302, 6676
BsuTUI	ATCGAT	1 cut(s)	5351
BtgI	CCRYGG	1 cut(s)	1768
BtgZI	GCGATG	1 cut(s)	2949
BtrI	CACGTC	1 cut(s)	5905
BtsI	GCAGTG	3 cut(s)	2301, 6152, 6523
BtuMI	TCGCGA	1 cut(s)	5106
BveI	ACCTGC	2 cut(s)	4206, 4242
CaiI	CAGNNNCTG	4 cut(s)	2897, 3450, 5050, 5699
CciI	TCATGA	3 cut(s)	285, 4690, 5512
CfoI	GCGC	9 cut(s)	1049, 1443, 1572, 1609, 3309, 3843, 3859, 5442, 6266
Cfr10I	RCCGGY	2 cut(s)	6204, 7398
ClaI	ATCGAT	1 cut(s)	5351
CseI	GACGC	1 cut(s)	1875
CsiI	ACCWGGT	1 cut(s)	1495
CspAI	ACCGGT	1 cut(s)	7398
CspCI	CAANNNNNGTGG	2 cut(s)	7087, 7122
DraI	TTTAAA	1 cut(s)	2677
DraIII	CACNNNGTG	2 cut(s)	4973, 6119
DriI	GACNNNNNGTC	2 cut(s)	2105, 3476
EaeI	YGGCCR	4 cut(s)	1419, 5447, 7128, 7231
Eam1104I	CTCTTC	6 cut(s)	2748, 2805, 3809, 4720, 6053, 7359
Eam1105I	GACNNNNNGTC	2 cut(s)	2105, 3476
EarI	CTCTTC	6 cut(s)	2748, 2805, 3809, 4720, 6053, 7359
Ecl136II	GAGCTC	1 cut(s)	5199
Eco105I	TACGTA	2 cut(s)	3937, 5179
Eco130I	CCWWGG	6 cut(s)	1305, 1404, 1768, 2867, 3757, 5613
Eco147I	AGGCCT	1 cut(s)	4302
Eco24I	GRGCYC	2 cut(s)	5201, 6499
Eco31I	GGTCTC	3 cut(s)	1394, 3618, 7208
Eco32I	GATATC	3 cut(s)	4082, 6251, 7096
Eco47I	GGWCC	8 cut(s)	960, 3654, 4350, 4854, 6780, 7033, 7068, 7121
Eco53kI	GAGCTC	1 cut(s)	5199
Eco57I	CTGAAG	9 cut(s)	774, 2919, 4019, 4536, 4542, 4906, 5027, 5076, 6690
Eco81I	CCTNAGG	1 cut(s)	3671
Eco88I	CYCGRG	1 cut(s)	1452
Eco91I	GGTNACC	2 cut(s)	6521, 7401
EcoICRI	GAGCTC	1 cut(s)	5199
EcoNI	CCTNNNNNAGG	4 cut(s)	1592, 2994, 6189, 7300
EcoO109I	RGGNCCY	1 cut(s)	4350
EcoO65I	GGTNACC	2 cut(s)	6521, 7401
EcoRI	GAATTC	2 cut(s)	5380, 6180
EcoRII	CCWGG	7 cut(s)	1258, 1495, 2626, 2680, 2956, 4629, 6830
EcoRV	GATATC	3 cut(s)	4082, 6251, 7096
EcoT14I	CCWWGG	6 cut(s)	1305, 1404, 1768, 2867, 3757, 5613
EcoT22I	ATGCAT	2 cut(s)	2455, 5818
EcoT38I	GRGCYC	2 cut(s)	5201, 6499
ErhI	CCWWGG	6 cut(s)	1305, 1404, 1768, 2867, 3757, 5613
FalI	AAGNNNNNCTT	2 cut(s)	3205, 3237
FaqI	GGGAC	9 cut(s)	1449, 1733, 2971, 4363, 4840, 5813, 6650, 6766, 6848
FauNDI	CATATG	6 cut(s)	985, 1066, 1223, 2149, 4850, 5818
FbaI	TGATCA	2 cut(s)	3547, 4687
FblI	GTMKAC	5 cut(s)	2031, 2608, 3246, 5175, 5234
FriOI	GRGCYC	2 cut(s)	5201, 6499
FspI	TGCGCA	4 cut(s)	1048, 1442, 1571, 3842
GlaI	GCGC	9 cut(s)	1048, 1442, 1571, 1608, 3308, 3842, 3858, 5441, 6265
GsaI	CCCAGC	1 cut(s)	5739
GsuI	CTGGAG	6 cut(s)	85, 3382, 5320, 5505, 5676, 6371
HapII	CCGG	8 cut(s)	686, 958, 995, 3558, 6012, 6205, 7399, 7433
HgaI	GACGC	1 cut(s)	1875
HhaI	GCGC	9 cut(s)	1049, 1443, 1572, 1609, 3309, 3843, 3859, 5442, 6266
Hin6I	GCGC	9 cut(s)	1047, 1441, 1570, 1607, 3307, 3841, 3857, 5440, 6264
HinP1I	GCGC	9 cut(s)	1047, 1441, 1570, 1607, 3307, 3841, 3857, 5440, 6264
HincII	GTYRAC	3 cut(s)	4660, 6571, 7027
HindII	GTYRAC	3 cut(s)	4660, 6571, 7027
HindIII	AAGCTT	7 cut(s)	2259, 2526, 3122, 3214, 4672, 5362, 6367
HpaI	GTTAAC	2 cut(s)	4660, 7027
HpaII	CCGG	8 cut(s)	686, 958, 995, 3558, 6012, 6205, 7399, 7433
Hpy99I	CGWCG	1 cut(s)	6532
HpyCH4IV	ACGT	5 cut(s)	2579, 3601, 3936, 5178, 5904
HpySE526I	ACGT	5 cut(s)	2579, 3601, 3936, 5178, 5904
HspAI	GCGC	9 cut(s)	1047, 1441, 1570, 1607, 3307, 3841, 3857, 5440, 6264
Kpn2I	TCCGGA	4 cut(s)	685, 957, 3557, 6011
Ksp22I	TGATCA	2 cut(s)	3547, 4687
KspAI	GTTAAC	2 cut(s)	4660, 7027
LguI	GCTCTTC	1 cut(s)	2805
MabI	ACCWGGT	1 cut(s)	1495
MaeII	ACGT	5 cut(s)	2579, 3601, 3936, 5178, 5904
MbiI	CCGCTC	2 cut(s)	249, 950
MfeI	CAATTG	6 cut(s)	1425, 1800, 1956, 3551, 3735, 5495
MflI	RGATCY	6 cut(s)	911, 2427, 3700, 4836, 6793, 6889
MhlI	GDGCHC	5 cut(s)	75, 4713, 5201, 6298, 6499
MlsI	TGGCCA	3 cut(s)	1421, 5449, 7130
MluNI	TGGCCA	3 cut(s)	1421, 5449, 7130
MlyI	GAGTC	9 cut(s)	3070, 3370, 3911, 4274, 5311, 5489, 5599, 6641, 6890
MmeI	TCCRAC	8 cut(s)	487, 3678, 4484, 4893, 6190, 6196, 6984, 7281
Mox20I	TGGCCA	3 cut(s)	1421, 5449, 7130
Mph1103I	ATGCAT	2 cut(s)	2455, 5818
MroI	TCCGGA	4 cut(s)	685, 957, 3557, 6011
MscI	TGGCCA	3 cut(s)	1421, 5449, 7130
Msp20I	TGGCCA	3 cut(s)	1421, 5449, 7130
MspA1I	CMGCKG	1 cut(s)	5699
MspI	CCGG	8 cut(s)	686, 958, 995, 3558, 6012, 6205, 7399, 7433
MspR9I	CCNGG	8 cut(s)	995, 1260, 1497, 2628, 2682, 2958, 4631, 6832
MunI	CAATTG	6 cut(s)	1425, 1800, 1956, 3551, 3735, 5495
Mva1269I	GAATGC	3 cut(s)	886, 1187, 3235
MvaI	CCWGG	7 cut(s)	1260, 1497, 2628, 2682, 2958, 4631, 6832
MvnI	CGCG	4 cut(s)	3490, 5106, 6264, 7031
NciI	CCSGG	1 cut(s)	995
NcoI	CCATGG	1 cut(s)	1768
NdeI	CATATG	6 cut(s)	985, 1066, 1223, 2149, 4850, 5818
NheI	GCTAGC	3 cut(s)	1157, 3709, 5558
NmeAIII	GCCGAG	1 cut(s)	4117
NmuCI	GTSAC	6 cut(s)	4277, 4844, 5682, 7076, 7172, 7401
NruI	TCGCGA	1 cut(s)	5106
NsbI	TGCGCA	4 cut(s)	1048, 1442, 1571, 3842
NsiI	ATGCAT	2 cut(s)	2455, 5818
NspI	RCATGY	5 cut(s)	2739, 3652, 5573, 6365, 7414
NspV	TTCGAA	1 cut(s)	1367
PaeI	GCATGC	1 cut(s)	2739
PaeR7I	CTCGAG	1 cut(s)	1452
PagI	TCATGA	3 cut(s)	285, 4690, 5512
PceI	AGGCCT	1 cut(s)	4302
PciSI	GCTCTTC	1 cut(s)	2805
PctI	GAATGC	3 cut(s)	886, 1187, 3235
PfoI	TCCNGGA	1 cut(s)	2626
PinAI	ACCGGT	1 cut(s)	7398
PleI	GAGTC	9 cut(s)	3069, 3370, 3910, 4274, 5310, 5488, 5598, 6641, 6889
PpsI	GAGTC	9 cut(s)	3069, 3370, 3910, 4274, 5310, 5488, 5598, 6641, 6889
Ppu21I	YACGTR	2 cut(s)	3937, 5179
PpuMI	RGGWCCY	1 cut(s)	4350
PshBI	ATTAAT	3 cut(s)	446, 1629, 6237
PsiI	TTATAA	2 cut(s)	2346, 3960
Psp124BI	GAGCTC	1 cut(s)	5201
Psp5II	RGGWCCY	1 cut(s)	4350
Psp6I	CCWGG	7 cut(s)	1258, 1495, 2626, 2680, 2956, 4629, 6830
PspEI	GGTNACC	2 cut(s)	6521, 7401
PspFI	CCCAGC	1 cut(s)	5735
PspGI	CCWGG	7 cut(s)	1258, 1495, 2626, 2680, 2956, 4629, 6830
PspPPI	RGGWCCY	1 cut(s)	4350
PstI	CTGCAG	4 cut(s)	1387, 4794, 4827, 6159
PstNI	CAGNNNCTG	4 cut(s)	2897, 3450, 5050, 5699
PsuI	RGATCY	6 cut(s)	911, 2427, 3700, 4836, 6793, 6889
PvuII	CAGCTG	1 cut(s)	5699
RruI	TCGCGA	1 cut(s)	5106
SacI	GAGCTC	1 cut(s)	5201
SapI	GCTCTTC	1 cut(s)	2805
ScaI	AGTACT	4 cut(s)	1381, 2785, 3458, 6736
SchI	GAGTC	9 cut(s)	3070, 3370, 3911, 4274, 5311, 5489, 5599, 6641, 6890
ScrFI	CCNGG	8 cut(s)	995, 1260, 1497, 2628, 2682, 2958, 4631, 6832
SduI	GDGCHC	5 cut(s)	75, 4713, 5201, 6298, 6499
SexAI	ACCWGGT	1 cut(s)	1495
SfcI	CTRYAG	7 cut(s)	1383, 4790, 4823, 5235, 5251, 5293, 6155
Sfr274I	CTCGAG	1 cut(s)	1452
SfuI	TTCGAA	1 cut(s)	1367
SinI	GGWCC	8 cut(s)	960, 3654, 4350, 4854, 6780, 7033, 7068, 7121
SlaI	CTCGAG	1 cut(s)	1452
SmlI	CTYRAG	6 cut(s)	201, 1452, 2182, 3094, 3182, 3344
SmoI	CTYRAG	6 cut(s)	201, 1452, 2182, 3094, 3182, 3344
SnaBI	TACGTA	2 cut(s)	3937, 5179
SpeI	ACTAGT	1 cut(s)	6218
SphI	GCATGC	1 cut(s)	2739
SseBI	AGGCCT	1 cut(s)	4302
SspI	AATATT	4 cut(s)	1296, 2278, 3026, 4321
SstI	GAGCTC	1 cut(s)	5201
StuI	AGGCCT	1 cut(s)	4302
StyD4I	CCNGG	8 cut(s)	993, 1258, 1495, 2626, 2680, 2956, 4629, 6830
StyI	CCWWGG	6 cut(s)	1305, 1404, 1768, 2867, 3757, 5613
TaiI	ACGT	5 cut(s)	2582, 3604, 3939, 5181, 5907
TaqII	GACCGA	2 cut(s)	6768, 7138
TatI	WGTACW	8 cut(s)	1379, 1902, 1980, 2783, 3456, 6510, 6734, 6951
TauI	GCSGC	2 cut(s)	4952, 7236
TseFI	GTSAC	6 cut(s)	4277, 4844, 5682, 7076, 7172, 7401
Tsp45I	GTSAC	6 cut(s)	4277, 4844, 5682, 7076, 7172, 7401
TspGWI	ACGGA	8 cut(s)	1094, 2657, 3284, 4832, 5863, 6107, 6965, 7064
VneI	GTGCAC	1 cut(s)	6294
VpaK11BI	GGWCC	8 cut(s)	960, 3654, 4350, 4854, 6780, 7033, 7068, 7121
VspI	ATTAAT	3 cut(s)	446, 1629, 6237
XagI	CCTNNNNNAGG	4 cut(s)	1592, 2994, 6189, 7300
XapI	RAATTY	8 cut(s)	2375, 3146, 3607, 4978, 5380, 5715, 5894, 6180
XbaI	TCTAGA	3 cut(s)	817, 908, 2695
XceI	RCATGY	5 cut(s)	2739, 3652, 5573, 6365, 7414
XcmI	CCANNNNNNNNNTGG	1 cut(s)	6173
XhoI	CTCGAG	1 cut(s)	1452
XmiI	GTMKAC	5 cut(s)	2031, 2608, 3246, 5175, 5234
ZrmI	AGTACT	4 cut(s)	1381, 2785, 3458, 6736
Zsp2I	ATGCAT	2 cut(s)	2455, 5818

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.