Rh6AG336100

Piezo non-specific cation channel, R-Ras-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0006607
Physical Location & Seq
Forward (+)
53646781 .. 53660309
13529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG336100.1

Sequence Viewer

Length: 7413 bp
ATGGGGAGATTTCTCGGTGGGTTTTTGTTGCCTCTGCTGCTGCTTGCAGCTGCTTTCATGCACTGGAGCTTGATCTCTCTTGGTGATCTCATATCTTTTCTTCTTATTCTATTTCATGCACCCAAAATAGGGTTTCATTTTGGAAGGAAGCTTTTATTGTTGTGGTTCGTCATCATATTTTCTTTATGTGCTATTTTTTCTCAAGTTCTATATCTGGTTCTATGGGCTATCGAGTGGAGTACAGCAGAAGAGTGGTGGGGAAAGCTCATTGGACTCTTGATAGCTGAGTCATGGAAATCACCATCTGTGGTATACTTTGTGGTCCTTCAACTCTCCATAGTTGCTGTTTCATTAGTTGATTTGTATGGGAACAGATTTGGTCCTTTTTCATCATTTGATTCGTGTTGGGGGCAATTCTTATCAACTGTTGATCGTTTAGTCTGTTCTCATATAAGGGTTGCTTCTTGCTTGCTGTTGCCTGCTATTCAACTGGTTGTTGGAATCAGCCATCCCTCATGGGTTTCTCTACCATTTTTTATTGGGAGCTGTGTTGGTCTTGTGGATTGGTCCTTGACGAGCAATTTTCTAGGACTTTTCAGGTGGTGGAGGCCTTTTCAATTGTATGCAGGCTTCAACATTGTCTTGCTTTATCTGTATCAGCTCCCTGTGGAGTTTCCAGATATGTTACAATGGGTAGCTGATTTCATTGGTCTCTTCAAAATAACTTCAGGTTCCCAATGGACCGAAGTTTGTTCAAGCTTCTCTCTTGTACTTTTTTACATAATGTTATCCTGTGTTAAATGTGATCTAGAGGAGATGGATTTTATTTTGTCTATGAAGGAAAATAACTTGACGGAGCATCTTCTTCCCTCAAAGCATTCATTTTTTATTCGTCAATCTAGATCTGGCGTAAGGCATACCAATGTCATGTTGACAGGGGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTATCCTTGGTTGCTCTTTCCTTCTGGAGTTTTCATTTTGCAAGTGTTTGTGCTTTTGGGCTACTTGCATATGTTGGCTACATCATATATGCATTCCCTTCCTTATTCCGTTTGCATCGACTGAATGGGCTGCTTCTTGTCTTCATTCTCTTGTGGGCTGTTAGCACATATATCTTCAATGTAGCTTTCACATTCTTGAATTGGAAAATTGGGAAGAACATGGACATATGGGAGATGGTTGGCTTGTGGCATTATCCCATACCTGGGTTCTTTTTGCTTGCTCAATTTTGTCTTGGAATGTTGGTTGCTTTGGGTAATCTTGTGAATAACTCAGTTTTCCTTTGCTTGTCTGATGAGGATGGACGATCATCAACTGACAATAGTACTATTGAAGGTGAGGGAGAGACCAAAGTATTGATTGTTGCCACAATAGCTTGGGGACTGCGCAAAAGCTCTCGGGCTATCATGCTGGCGCTAATATTTCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTGTATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACGTTAACAGAAAATTACGCCAGTCTTTGATTCTCCTATGCGAGGTTCACTTTGCACTATTGTATATCATTCGGATCAATCCGATCTCCAGTAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCTTATTACAGTTAGGTCTCCTTGAAAAGGAAAGCTCGTGGGATTTCTTGGAAATAGCTTTGCTTGCTTGTTCCTGTGCAATTCATAACCATGGTTTTGAAATGCTTTTTTCGTTCTCAGCAATCGTGCAACATACACCTAGCCTTCCATTTGGATTTGGCATTTTGAAAGCTGGTCTGAACAAATCAGTATTATTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTATTCCTCAGTGCAATCGGGCAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAGACCAAATTATATCTCATTTGGGTACATATCTCTTCTCCTCGTTTGGATAATTGGAAGACAACTTGTTGAGAGAACGAAAAAGCGCTTGTGGTTCCCATTAAAAGCATATTCAATTACAGTATTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGTTTGGTTGTCCAGGTTGATAGACCTTGATTTCTATCTGGGGTATGACTCAAAAGCTTCGTCTTTGGAAAATGTTTGGGAATCCTTAGCAGTCTTGATTGTGATGCAACTTTACAGCTATGAGAGGAGGCAGAGCAGGTACAACAGGTCAGATGATCCTGATTTGACAGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTGTCTCCAATTTGTGCGTTTAGCTTTTTGTATCTCCTTGGCCTTGTCATCTGTTCAACTTTACCTAAAGCTTCACGAATCCCATCTAAATCATTCTTGGCTTACACAGGATTTCTAGTAACAGCAGAGTATCTTTTTCAGATGTTGGGTAGCCAGGCTGCAATGTTTCCTGGACAAAAGCACTCTAATATTGCTCTTCTTTTGGGTTTCCGTGAATTTAAAGCTGGCTTCTGGGGTCTAGAATCTGGATTGAGAGGAAAAGTGCTGGTGATTGCTGCATGCACTCTTCAGTATAATGTTTTCCACTGGTTGGAGAAGATGCCAAGTACTATTCTAAGCAATGGAATGGGAGAGCCTTGTCCATTGTTTGTGTCAGCAGAAGATACAAATATTAGTGCTCCTATCCCTAGTGAGGATAACAGGCCATCAACTCCACTTTCTGTGAAGCGAGAAGCGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTGGTCTGTTGCAATCACTCAATCCCTCAACCCCAAAAGCAGGAAGTTCTGAGGGTAGTAGCGGTAGCAAATATTCATTTGGGTATATTTGGGGAAGCACCAAGGAGAGCCATAAGTGGAATACGAAAAAAATCCTTGCCTTGCGAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATGAAATTCTGGATGGAGAATTTGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCTCTGCTTCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATAGGCAGATTATACGCAAGTTATGGCCAATATTCGTCTTCCTGTTTGCTTCCATTCTCGTTCTTGAGTATTTTGCCATCTGGAAGAGTGCTTGGCCTCCAAATCATCCTGATGCAACTAATCCACATTGCCATGATTGCTGGCAAAAGTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTAATGTTGATGATCCTCGAATGCTTATCAGCTATTTTATTGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCGGGCTTATCAACGTACCGTGAGATGATATCTCAACGTGAAAATACATTTGTATGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTCTACTGCTATTGCCATCTACTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACCATTGAGTATGACATCCTGCACCTTGGTTATCTCGCTTTTGCACTGGTTTTCTTTCGGGTTAGACTTCAAATACTAAAGAAAAGGAACAAGATGTTCAAGTTCTTGCGCATATACAATTTTGCTCTCATTGTTCTATCTCTTGCATATCAATCTCCTTTTGTGGGTTGTTCTGGGAAGTGTGAGACCATATACGAGATGATTGGCTTTTATAAATATGACTATGGGTTTCAGATGACTGCAAGATCAGCTCTCGTTGAAATTATCATATTTATGCTGGTATCACTTCAGTCATATATGTTTTCCTCTAAAGAGTTTGATTATGTGTCTCGATACCTTGAAGCTGAACAAATTGGTATGATTGTGCACGAACAAGAAAAAAAAGCTGCATGGAAAACTGCACAATTACGACATATTCGTGAATCTGAGGAGAAGAAACGCCAGCGTAACCTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCAGCTTCACAGTACGAACTCAGTTACTGACTGCGGTGACTCTTCTCCTGTCAGTGAAGGCCTAAGAAGGAGGAGAAGTACTTCTCTTAACTTGAACAATGATGCTTGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGAGACTTCTAGCAATGAATTGCATGAATCTCCTGCAACAGGGAATCTGGAAAACCTCACAGTTGTGGATTCTATGAAAAATTCAATGCAATCTATTCATTGTGAGATCACCGAAATTGAAGAGGACGTTGCTGATGGTACATTTACTGATTCAGAGAAGAAAGAGAAGTGTAAAGGGAAATCAACGGATAACCCTCTAATATCAGCAGTTCATCTGATAGGTGATGGTGTTTCCCAGGTACAGTCTATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTGAATATTGACCAAGAATCCGATATCCATGAGCACTCTGCTGAGGATGGGGTATATGATGAGATGGAGAGCCAGAAAATAAAGCACGCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATATGAGTTCTGATGCCACAAGTCTGCAGTTAGGAAGGATCTTCCGTCACATATGGTCCCGAATGCGGTCCAATAATGATGTTGTCTGTTATTGTTGCTTTGTCATCGTCTTTTTGTGGAACTTCAGTTTGCTTTCCATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTGTGTGTTAATTCTGGTCCAAGTTACATATTCTGGGTTATCATGCTCATATACACAGAAGTTTATATTTTGCTTCTGTATCTATACCAGATTATCATCCAGCACTATGGTTCGCGTATTGCTTCAGACCTACTTCGTGAGTTGGGATTTCCTGCACGTAAAATGTCTTCATTTGTTATCAGTTCGTTGCCCCTATTTCTCGTGTACTTATTCACCCTTGTCCAGAGCTCTATAACCGCAAAGGATGGCGAGTGGATGTCTTCAACAGACTTCAACTTCTATACGAGGAACGCTGTGCAGGGAAAAGAGGTTCCTGTCAGTTATAGCTGGAGTGAGAGGGCAAAGGACCCGCTGCACATAATTGTAAATGTCATACAGTTGATATGTAGAAGCTTCTTTAGGTACTGGGTATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTGTTCAGGTTTCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCAGAAAGGATTGAGTCTGGAGTAAATCAATTGCTTAGAATCATCCATGATGAAAGATGCAAGGCAAAGGATCCTAAGCGATGCCCTTTTGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCGAGAAAATGCAAATGTGGCCTTGGTCGTTTTTGAGGTAGTATATGCCTCCCCCATAACAGACTGTGGTTCGGTAGAATGGTACAAGTCACTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCTTAAAGCACTGCATGCTGGCTTTGTTGAAGAAATAGGATTCCCATATCCTATACTCTCTGTTATCGGGGGAGGCAAAAGGGACATTGATCTGTTTGCCTATGTATTTGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTCCATTATAAAAAATAAAAGTGACTTTCTTGACGTGTATCAGCTTGAAGATCAATTTCCTAAAGAGTTCGTCTTCATCTTAATGATTATTTTTTTCTTGATTGTCCTTGATCGAATAATCTACCTCTGTTCATTTGCCACTGGAAAAGTGATTTACTACCTTTTTAACCTCATCCTATTCACGTATTCAGTTACTAAGTATGCTTGGCATATGGAATCTTCCCGCCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAATCAATATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATATCACGAATTAATTACTTAGGATATCGACTATATCGTGCTCTGCCGTTCCTTTATGAATTGAGATGTGTACTTGACTGGTCATGTACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTCGTCAAATGTGATGCAGTCCTGAATAGAGCAACGCACAAACAAGGAGAAAAGCAAACACAAATGACCAAATGTTGCAATGGGATTTGTCTTTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCCATTAAAGATGCCAGTGTTCAAGTTGATATCAAGACAATGAGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATTGATTGGGATAATGTCAACACTGATGTTAATCTTGACCCTCAAGGTTATTTGGAGACATATAACAAGAAAGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCTGATGTCGTTCAGACCAGATTCATACGGTCCCTCAATTGGGAGAGCAACATGGCTATAACTTTTACTTGGGTACTTTCCAGGGAGAGACCCAAAGACAAGGAAGTTGTGAAATATGAAAATAATCTTGGTGTTGAGGATCTTCCAAAACAATCAGATGTACAAAAAGTCCTTAATGGCTCTATCAACAGCTTTAGGATACACAATGTCTATCCAAGATACTTGCGTGTCACAGGTTCTGGTGATGTCAGGCCACTGGAAGCAGGGGAAACTTTTGTCACTGCGGACCTCGTTATAAATCGCGCTAGTTACCAGTGGTGGTGGTCCTTCAGTGACATCAATTCATCAGATGTCAATGGATGTGGCGGTTTGAGAGGACCCATGGCCATTATTATGTCTGAAGAAACGCCGCCACAGGGTATTCTTGGTGACACACTAAGCAAGTTCAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCCTATGAAAATCTTCCCTCCTGTGATAGGTTGTTGGCCATTTGTGAGGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGTATTGAAGAAATCCTTTACTGGACACTAGTGAAGATTTACAGATCACCACACATGCTGCTAGAGTACACAAAACCTGACTAG

Protein Analysis

2470

Amino Acids

282.47

Weight (kDa)

7.65

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIEZO PF25288 469 - 598 3.2e-62 Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1 PF23188 1613 - 1731 4.1e-15 Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor PF24874 1924 - 2161 1.3e-65 Piezo, THU9 and anchor domain
Piezo_cap PF12166 2189 - 2469 3.4e-77 Piezo non-specific cation channel, cap domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 3927, 5843, 7013
Acc16I TGCGCA 2 cut(s) 1433, 3824
Acc36I ACCTGC 2 cut(s) 2322, 4173
AccB7I CCANNNNNTGG 3 cut(s) 2758, 3679, 5423
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 5053, 7020
AccIII TCCGGA 1 cut(s) 948
AclI AACGTT 1 cut(s) 1545
AclWI GGATC 8 cut(s) 1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864
AcoI YGGCCR 7 cut(s) 2921, 3287, 5414, 5820, 7101, 7204, 7284
AcsI RAATTY 6 cut(s) 2366, 2663, 3128, 3142, 4444, 6147
AcuI CTGAAG 9 cut(s) 711, 940, 2720, 3986, 4876, 5046, 7030, 7137, 7205
AdeI CACNNNGTG 2 cut(s) 4943, 6086
AfeI AGCGCT 1 cut(s) 2117
AflIII ACRYGT 1 cut(s) 5868
AhlI ACTAGT 1 cut(s) 7357
AjiI CACGTC 1 cut(s) 5869
AjnI CCWGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
AjuI GAANNNNNNNTTGG 2 cut(s) 6828, 6860
AleI CACNNNNGTG 1 cut(s) 4427
AloI GAACNNNNNNTCC 4 cut(s) 3131, 3163, 5232, 5264
Alw21I GWGCWC 8 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6581, 7229
Alw44I GTGCAC 1 cut(s) 4079
AlwI GGATC 8 cut(s) 1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864
AlwNI CAGNNNCTG 5 cut(s) 3432, 4235, 5663, 6956, 7170
Ama87I CYCGRG 2 cut(s) 1443, 3547
Aor13HI TCCGGA 1 cut(s) 948
Aor51HI AGCGCT 1 cut(s) 2117
ApaLI GTGCAC 1 cut(s) 4079
ApoI RAATTY 6 cut(s) 2366, 2663, 3128, 3142, 4444, 6147
ArsI GACNNNNNNTTYG 4 cut(s) 2240, 2272, 3599, 3631
AseI ATTAAT 1 cut(s) 6204
Asp700I GAANNNNTTC 5 cut(s) 2914, 3146, 4288, 4335, 7260
AspLEI GCGC 5 cut(s) 1434, 1462, 2118, 3825, 7022
AsuNHI GCTAGC 1 cut(s) 5525
AvaI CYCGRG 2 cut(s) 1443, 3547
BaeGI GKGCMC 1 cut(s) 4083
BaeI ACNNNNGTAYC 2 cut(s) 6928, 6961
BalI TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
BamHI GGATCC 1 cut(s) 5503
BanII GRGCYC 2 cut(s) 5168, 6466
BarI GAAGNNNNNNTAC 6 cut(s) 614, 646, 1659, 1691, 5089, 5121
BauI CACGAG 2 cut(s) 1705, 5138
BbsI GAAGAC 7 cut(s) 1120, 2095, 3292, 3490, 5097, 5190, 5899
Bbv12I GWGCWC 8 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6581, 7229
BbvCI CCTCAGC 1 cut(s) 4689
BceAI ACGGC 1 cut(s) 6223
BciT130I CCWGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
BciVI GTATCC 3 cut(s) 1000, 5421, 6909
BclI TGATCA 1 cut(s) 3529
BcuI ACTAGT 1 cut(s) 7357
BfmI CTRYAG 3 cut(s) 2167, 4760, 4793
BfoI RGCGCY 2 cut(s) 1463, 2119
BfuAI ACCTGC 2 cut(s) 2322, 4173
BfuI GTATCC 3 cut(s) 1000, 5421, 6909
BglII AGATCT 2 cut(s) 902, 2418
BmcAI AGTACT 4 cut(s) 1372, 2776, 3440, 4288
Bme1390I CCNGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
BmeT110I CYCGRG 2 cut(s) 1443, 3547
BmgBI CACGTC 1 cut(s) 5869
BmrFI CCNGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
BmrI ACTGGG 1 cut(s) 5353
BmtI GCTAGC 1 cut(s) 5529
BmuI ACTGGG 1 cut(s) 5353
BpiI GAAGAC 7 cut(s) 1120, 2095, 3292, 3490, 5097, 5190, 5899
BpmI CTGGAG 8 cut(s) 85, 1033, 1615, 5287, 5472, 5640, 6090, 6338
Bpu10I CCTNAGC 3 cut(s) 2281, 4689, 5508
BpuEI CTTGAG 5 cut(s) 186, 2194, 3185, 3347, 6621
BsaAI YACGTR 2 cut(s) 5096, 6018
BsaBI GATNNNNATC 3 cut(s) 2229, 5033, 6624
BsaI GGTCTC 8 cut(s) 716, 1385, 1691, 2026, 3600, 3893, 6799, 7181
BsaWI WCCGGW 2 cut(s) 948, 985
BsaXI ACNNNNNCTCC 4 cut(s) 3131, 3161, 4270, 4300
Bse8I GATNNNNATC 3 cut(s) 2229, 5033, 6624
BseAI TCCGGA 1 cut(s) 948
BseBI CCWGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
BseJI GATNNNNATC 3 cut(s) 2229, 5033, 6624
BseMII CTCAG 8 cut(s) 276, 1332, 1800, 1954, 2976, 4131, 4242, 4680
BseRI GAGGAG 6 cut(s) 827, 1870, 2060, 2335, 4157, 4294
BseSI GKGCMC 1 cut(s) 4083
BseYI CCCAGC 1 cut(s) 2186
BsgI GTGCAG 6 cut(s) 1683, 3719, 4098, 5076, 5255, 5276
Bsh1236I CGCG 2 cut(s) 5053, 7020
BsiHKAI GWGCWC 8 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6581, 7229
BsiHKCI CYCGRG 2 cut(s) 1443, 3547
BsiSI CCGG 2 cut(s) 949, 986
BslFI GGGAC 5 cut(s) 1440, 4810, 5356, 5777, 6733
BsmFI GGGAC 5 cut(s) 1440, 4810, 5356, 5777, 6733
BsmI GAATGC 5 cut(s) 877, 1079, 3217, 3480, 4836
Bso31I GGTCTC 8 cut(s) 716, 1385, 1691, 2026, 3600, 3893, 6799, 7181
BsoBI CYCGRG 2 cut(s) 1443, 3547
Bsp1286I GDGCHC 9 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6466, 6581, 7229
Bsp13I TCCGGA 1 cut(s) 948
Bsp1407I TGTACA 3 cut(s) 5539, 6278, 6877
Bsp19I CCATGG 3 cut(s) 1759, 4905, 7098
BspCNI CTCAG 8 cut(s) 277, 1331, 1799, 1953, 2977, 4132, 4241, 4681
BspEI TCCGGA 1 cut(s) 948
BspFNI CGCG 2 cut(s) 5053, 7020
BspMAI CTGCAG 2 cut(s) 4764, 4797
BspMI ACCTGC 2 cut(s) 2322, 4173
BspOI GCTAGC 1 cut(s) 5529
BspPI GGATC 8 cut(s) 1625, 2345, 3461, 3690, 4814, 5498, 5511, 6864
BspQI GCTCTTC 1 cut(s) 2649
BspTNI GGTCTC 8 cut(s) 716, 1385, 1691, 2026, 3600, 3893, 6799, 7181
BsrGI TGTACA 3 cut(s) 5539, 6278, 6877
BssNAI GTATAC 1 cut(s) 313
BssSI CACGAG 2 cut(s) 1705, 5138
BssT1I CCWWGG 9 cut(s) 993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
Bst1107I GTATAC 1 cut(s) 313
Bst2BI CACGAG 2 cut(s) 1705, 5138
Bst2UI CCWGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
Bst6I CTCTTC 8 cut(s) 243, 719, 2070, 2649, 2739, 3341, 4255, 4479
BstAPI GCANNNNNTGC 5 cut(s) 3200, 3242, 3441, 5696, 6086
BstAUI TGTACA 3 cut(s) 5539, 6278, 6877
BstBAI YACGTR 2 cut(s) 5096, 6018
BstDSI CCRYGG 3 cut(s) 1759, 4905, 7098
BstEII GGTNACC 1 cut(s) 6488
BstENI CCTNNNNNAGG 4 cut(s) 1583, 1694, 4401, 7273
BstFNI CGCG 2 cut(s) 5053, 7020
BstH2I RGCGCY 2 cut(s) 1463, 2119
BstHHI GCGC 5 cut(s) 1434, 1462, 2118, 3825, 7022
BstNI CCWGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
BstNSI RCATGY 7 cut(s) 2730, 3634, 5540, 5699, 6068, 6332, 7387
BstPI GGTNACC 1 cut(s) 6488
BstSCI CCNGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
BstSFI CTRYAG 3 cut(s) 2167, 4760, 4793
BstSLI GKGCMC 1 cut(s) 4083
BstUI CGCG 2 cut(s) 5053, 7020
BstV2I GAAGAC 7 cut(s) 1120, 2095, 3292, 3490, 5097, 5190, 5899
BstX2I RGATCY 6 cut(s) 902, 2418, 3682, 4806, 5503, 6856
BstXI CCANNNNNNTGG 3 cut(s) 1639, 5045, 6068
BstYI RGATCY 6 cut(s) 902, 2418, 3682, 4806, 5503, 6856
BstZ17I GTATAC 1 cut(s) 313
BsuI GTATCC 3 cut(s) 1000, 5421, 6909
BtgI CCRYGG 3 cut(s) 1759, 4905, 7098
BtgZI GCGATG 1 cut(s) 5527
BtrI CACGTC 1 cut(s) 5869
BtsI GCAGTG 4 cut(s) 5690, 6119, 6490, 6996
BveI ACCTGC 2 cut(s) 2322, 4173
CaiI CAGNNNCTG 5 cut(s) 3432, 4235, 5663, 6956, 7170
CfoI GCGC 5 cut(s) 1434, 1462, 2118, 3825, 7022
CsiI ACCWGGT 1 cut(s) 1486
CspCI CAANNNNNGTGG 2 cut(s) 7060, 7095
DraI TTTAAA 1 cut(s) 2668
DraIII CACNNNGTG 2 cut(s) 4943, 6086
EaeI YGGCCR 7 cut(s) 2921, 3287, 5414, 5820, 7101, 7204, 7284
Eam1104I CTCTTC 8 cut(s) 243, 719, 2070, 2649, 2739, 3341, 4255, 4479
EarI CTCTTC 8 cut(s) 243, 719, 2070, 2649, 2739, 3341, 4255, 4479
Ecl136II GAGCTC 1 cut(s) 5166
Eco130I CCWWGG 9 cut(s) 993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
Eco147I AGGCCT 2 cut(s) 610, 4269
Eco24I GRGCYC 2 cut(s) 5168, 6466
Eco31I GGTCTC 8 cut(s) 716, 1385, 1691, 2026, 3600, 3893, 6799, 7181
Eco32I GATATC 4 cut(s) 3576, 4672, 6218, 6541
Eco47III AGCGCT 1 cut(s) 2117
Eco53kI GAGCTC 1 cut(s) 5166
Eco57I CTGAAG 9 cut(s) 711, 940, 2720, 3986, 4876, 5046, 7030, 7137, 7205
Eco88I CYCGRG 2 cut(s) 1443, 3547
Eco91I GGTNACC 1 cut(s) 6488
EcoICRI GAGCTC 1 cut(s) 5166
EcoNI CCTNNNNNAGG 4 cut(s) 1583, 1694, 4401, 7273
EcoO109I RGGNCCY 2 cut(s) 5284, 7094
EcoO65I GGTNACC 1 cut(s) 6488
EcoRI GAATTC 1 cut(s) 6147
EcoRII CCWGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
EcoRV GATATC 4 cut(s) 3576, 4672, 6218, 6541
EcoT14I CCWWGG 9 cut(s) 993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
EcoT22I ATGCAT 2 cut(s) 1081, 2446
EcoT38I GRGCYC 2 cut(s) 5168, 6466
ErhI CCWWGG 9 cut(s) 993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
FalI AAGNNNNNCTT 6 cut(s) 445, 477, 3187, 3219, 7329, 7361
FaqI GGGAC 5 cut(s) 1440, 4810, 5356, 5777, 6733
FauI CCCGC 2 cut(s) 5295, 6065
FauNDI CATATG 5 cut(s) 976, 1057, 1214, 4820, 6045
FbaI TGATCA 1 cut(s) 3529
FblI GTMKAC 1 cut(s) 312
FriOI GRGCYC 2 cut(s) 5168, 6466
FspI TGCGCA 2 cut(s) 1433, 3824
GlaI GCGC 5 cut(s) 1433, 1461, 2117, 3824, 7021
GsaI CCCAGC 1 cut(s) 2190
GsuI CTGGAG 8 cut(s) 85, 1033, 1615, 5287, 5472, 5640, 6090, 6338
HaeII RGCGCY 2 cut(s) 1463, 2119
HapII CCGG 2 cut(s) 949, 986
HhaI GCGC 5 cut(s) 1434, 1462, 2118, 3825, 7022
Hin6I GCGC 5 cut(s) 1432, 1460, 2116, 3823, 7020
HinP1I GCGC 5 cut(s) 1432, 1460, 2116, 3823, 7020
HincII GTYRAC 6 cut(s) 933, 1549, 3414, 4630, 6564, 6613
HindII GTYRAC 6 cut(s) 933, 1549, 3414, 4630, 6564, 6613
HindIII AAGCTT 7 cut(s) 149, 757, 2250, 2517, 3196, 5329, 6334
HpaI GTTAAC 2 cut(s) 1549, 4630
HpaII CCGG 2 cut(s) 949, 986
HpyCH4IV ACGT 7 cut(s) 1545, 3560, 3583, 4491, 5095, 5868, 6017
HpySE526I ACGT 7 cut(s) 1545, 3560, 3583, 4491, 5095, 5868, 6017
HspAI GCGC 5 cut(s) 1432, 1460, 2116, 3823, 7020
Kpn2I TCCGGA 1 cut(s) 948
Ksp22I TGATCA 1 cut(s) 3529
KspAI GTTAAC 2 cut(s) 1549, 4630
LguI GCTCTTC 1 cut(s) 2649
MabI ACCWGGT 1 cut(s) 1486
MaeII ACGT 7 cut(s) 1545, 3560, 3583, 4491, 5095, 5868, 6017
MfeI CAATTG 3 cut(s) 617, 5462, 6754
MflI RGATCY 6 cut(s) 902, 2418, 3682, 4806, 5503, 6856
MhlI GDGCHC 9 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6466, 6581, 7229
MlsI TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
MluNI TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
MlyI GAGTC 7 cut(s) 267, 296, 2237, 4241, 5378, 5456, 5566
MmeI TCCRAC 2 cut(s) 478, 2739
Mox20I TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
Mph1103I ATGCAT 2 cut(s) 1081, 2446
MroI TCCGGA 1 cut(s) 948
MroXI GAANNNNTTC 5 cut(s) 2914, 3146, 4288, 4335, 7260
MscI TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
Msp20I TGGCCA 6 cut(s) 2923, 3289, 5416, 5822, 7103, 7286
MspA1I CMGCKG 3 cut(s) 50, 5290, 5663
MspI CCGG 2 cut(s) 949, 986
MspR9I CCNGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
MunI CAATTG 3 cut(s) 617, 5462, 6754
Mva1269I GAATGC 5 cut(s) 877, 1079, 3217, 3480, 4836
MvaI CCWGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
MvnI CGCG 2 cut(s) 5053, 7020
NcoI CCATGG 3 cut(s) 1759, 4905, 7098
NdeI CATATG 5 cut(s) 976, 1057, 1214, 4820, 6045
NheI GCTAGC 1 cut(s) 5525
NmuCI GTSAC 8 cut(s) 4244, 4814, 5646, 5855, 6946, 6994, 7049, 7145
NsbI TGCGCA 2 cut(s) 1433, 3824
NsiI ATGCAT 2 cut(s) 1081, 2446
NspI RCATGY 7 cut(s) 2730, 3634, 5540, 5699, 6068, 6332, 7387
OliI CACNNNNGTG 1 cut(s) 4427
PaeI GCATGC 2 cut(s) 2730, 5699
PceI AGGCCT 2 cut(s) 610, 4269
PciSI GCTCTTC 1 cut(s) 2649
PcsI WCGNNNNNNNCGW 2 cut(s) 4129, 6226
PctI GAATGC 5 cut(s) 877, 1079, 3217, 3480, 4836
PdmI GAANNNNTTC 5 cut(s) 2914, 3146, 4288, 4335, 7260
PflMI CCANNNNNTGG 3 cut(s) 2758, 3679, 5423
PfoI TCCNGGA 1 cut(s) 2617
PleI GAGTC 7 cut(s) 267, 295, 2237, 4241, 5377, 5455, 5565
PpsI GAGTC 7 cut(s) 267, 295, 2237, 4241, 5377, 5455, 5565
Ppu21I YACGTR 2 cut(s) 5096, 6018
PpuMI RGGWCCY 2 cut(s) 5284, 7094
PshBI ATTAAT 1 cut(s) 6204
PsiI TTATAA 3 cut(s) 3927, 5843, 7013
Psp124BI GAGCTC 1 cut(s) 5168
Psp1406I AACGTT 1 cut(s) 1545
Psp5II RGGWCCY 2 cut(s) 5284, 7094
Psp6I CCWGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
PspEI GGTNACC 1 cut(s) 6488
PspFI CCCAGC 1 cut(s) 2186
PspGI CCWGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
PspPPI RGGWCCY 2 cut(s) 5284, 7094
PstI CTGCAG 2 cut(s) 4764, 4797
PstNI CAGNNNCTG 5 cut(s) 3432, 4235, 5663, 6956, 7170
PsuI RGATCY 6 cut(s) 902, 2418, 3682, 4806, 5503, 6856
PvuII CAGCTG 2 cut(s) 50, 5663
SacI GAGCTC 1 cut(s) 5168
SapI GCTCTTC 1 cut(s) 2649
ScaI AGTACT 4 cut(s) 1372, 2776, 3440, 4288
SchI GAGTC 7 cut(s) 267, 296, 2237, 4241, 5378, 5456, 5566
ScrFI CCNGG 9 cut(s) 1251, 1488, 2209, 2603, 2619, 2937, 4601, 5413, 6799
SduI GDGCHC 9 cut(s) 2848, 4083, 4683, 5168, 5411, 6235, 6466, 6581, 7229
SexAI ACCWGGT 1 cut(s) 1486
SfcI CTRYAG 3 cut(s) 2167, 4760, 4793
SmlI CTYRAG 5 cut(s) 201, 2173, 3164, 3326, 6636
SmoI CTYRAG 5 cut(s) 201, 2173, 3164, 3326, 6636
SpeI ACTAGT 1 cut(s) 7357
SphI GCATGC 2 cut(s) 2730, 5699
SseBI AGGCCT 2 cut(s) 610, 4269
SspI AATATT 6 cut(s) 1467, 2638, 2839, 3008, 3294, 4654
SstI GAGCTC 1 cut(s) 5168
StuI AGGCCT 2 cut(s) 610, 4269
StyD4I CCNGG 9 cut(s) 1249, 1486, 2207, 2601, 2617, 2935, 4599, 5411, 6797
StyI CCWWGG 9 cut(s) 993, 1759, 2485, 3036, 3696, 3739, 4905, 5580, 7098
TaiI ACGT 7 cut(s) 1548, 3563, 3586, 4494, 5098, 5871, 6020
TaqI TCGA 7 cut(s) 231, 1105, 3472, 4045, 5560, 5947, 6220
TaqII GACCGA 1 cut(s) 758
TauI GCSGC 3 cut(s) 2906, 7129, 7209
TseFI GTSAC 8 cut(s) 4244, 4814, 5646, 5855, 6946, 6994, 7049, 7145
Tsp45I GTSAC 8 cut(s) 4244, 4814, 5646, 5855, 6946, 6994, 7049, 7145
TspGWI ACGGA 6 cut(s) 869, 1085, 2648, 4565, 4802, 6074
Van91I CCANNNNNTGG 3 cut(s) 2758, 3679, 5423
VneI GTGCAC 1 cut(s) 4079
VspI ATTAAT 1 cut(s) 6204
XagI CCTNNNNNAGG 4 cut(s) 1583, 1694, 4401, 7273
XapI RAATTY 6 cut(s) 2366, 2663, 3128, 3142, 4444, 6147
XbaI TCTAGA 3 cut(s) 808, 899, 2686
XceI RCATGY 7 cut(s) 2730, 3634, 5540, 5699, 6068, 6332, 7387
XcmI CCANNNNNNNNNTGG 1 cut(s) 4912
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 5 cut(s) 2914, 3146, 4288, 4335, 7260
ZrmI AGTACT 4 cut(s) 1372, 2776, 3440, 4288
Zsp2I ATGCAT 2 cut(s) 1081, 2446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.