Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0006607

Physical Location & Seq

Genomic Coordinates Reverse (-)

15070458 .. 15084013

Gene Structure 13556 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00175510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7116 bp

ATGGGGAGATTTCTCGGTGGGTTTTTGTTGCCTCTGCTGCTGCTTGCAGCTGCTTTCATGCACTGGAGCTTGATCTCTCTTGGTGATCTCATATCTTTTCTTCTTATTCTATTTCATGCACCCAAAATAGGAGGCTTTTATTGTTGTGGTTCATCATCATATTTTCTTTCTGTGCTATTTTTTCTCAAGTATCTATATCTGGTTCTATGGGCCATCGAGTGGAGTGTAGCCGAAGAGTGGTGGGGAAAGCTCATTGGACTCTTGATAGCTGAGTCATGGAAATCACCATCTGTGGTATACTTTATGGTCCTTCAACTCTCCATAGTTGCTGTTTCATTGGTTGATTTGTATGGGAACAGATTTGGTCTTTTTTCATCATTTGATTCATGTTGGGGGCAATTCTTATCAACTGTTGATCGCTTAGTCTGCTTCAACATTGTCTTGCTTTATCTGTATCAGCTCCCTGTGGAGTTTCCAGATATGTTACAATGGGTAGCTGATTTCATTGGTCTCTTCAAAATAACTTCAGGTTCCCAATGGACCGAAGTTTGTTCAAGCTTCTCTCTTGTACTTTTTTACATAATGTTATCCTGTGTTAAATGTGATCTAGAGGAGATGGATTTTATTTTGTCTATGAAAGAAAATAACTTGACGGAGCATCTTCTTCCCTCAAAGCATTCATTTTTTATTCGTCAATCTAGATCGGGCGTAAGGCATACCAATGTCATGTTGACAGGGGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTATCCTTGGTTGCTCTTTCCTTCTGGAGTTTTCATTTTGCAAGTGTTTGTGCTTTTGGGCTACTTGCATATGTTGGCTACATCATATATGCATTCCCTTCCTTATTCCGTTTGCATCGACTGAATGGGCTGCTTCTTGTCTTCATTCTCTTGTGGGCTGTTAGCACATATATCTTCAATGTAGCTTTCACATTCTTGAATTGGAAAATTGGGAAGAACATGGACATATGGGAGATGGTTGGCTTGTGGCATTATCCCATACCTGGATTCTTTTTGCTTGCTCAATTTTGTCTTGGAATGTTGGTTGCTTTGGGTAATCTTGTGAATAACTCAGTTTTCCTTTGCTTGTCTGATGAGGATGGGCGATCATCAACTGACAATAGTACTATTGAAGGTGAGGGAGAGACCAAAGTATTGATTGTTGCCACAATAGCTTGGGGACTGCGGAAAAGCTCTCGGGCTATCATGCTGGCGCTAATATTTCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTGTATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACGTTAACAGAAAATTACGCCAGTCTTTGATTCTCCTATGCGAGGTTCACTTTGCACTATTGTATATCATTCGGATCAATCTGATCTCCAGTAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCTTATTACAGTTAGGTCTCCTTGAAAAGGAAAGCTCGTGGGATTTCTTGGAAATAGCTTTGCTTGCTTGTTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTTTTTTCGTTCTCAGCAATCGTGCAACATACACCTAGCCTTCCATTTGGATTTGGCATTTTGAAAGCTGGTCTGAACAAATCAGTATTGTTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTATTCCTCAGTGCAATCGGGCAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAAACCGAATTATATCTCATTTGGGTACATATCTCTTCTCCTCGTTTGGATAATTGGAAGACAACTTGTTGAGAGAACGAAAAAGCGCTTGTGGTTCCCATTAAAAGCATATTCAATTACAGTATTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGTTTGGTTGTCCAGGTTGATAGACCTTGATTTCTATCTGGGGTACGACTCAAAAGCTTCGTCTTTGGAAAATGTTTGGGAATCCCTAGCGGTCTTGATTGTGATGCAACTTTACAGCTACGAGAGGAGGCAGAGCAGGTACAACAGGTCAGATGATCCTGATTTGACAGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTGTCTCCAATTTGTGCGTTTAGCTTTTTGTATCTCCTTGGCCTTGTCATCTGTTCAACTTTACCTAAAGCTTCACGAATCCCGTCTAAATCATTCTTGGCTTACACAGGATTTCTAGTAACAGCAGAGTATCTTTTTCAGATGTTGGGTGGCCAGGCTGCAATGTTTCCTGGACAAAAGCACTCTAATATTGCTCTTCTTTTGGGTTTCCGTGAATTTAAGGCTGGCTTCTGGGGTCTAGAATCTGGATTGAGAGGAAAAGTGCTGGTGATTTCTGCATGCACTCTTCAGTATAATGTTTTCCGCTGGTTGGAGAAGATGCCGAGTACTATTCTAAGCAATGGAATGGGAGAGCCTTGTCCATTGTTTGTGTCAGCAGAAGATACAAATATTCGTGCTCCTACCCCTAGTGAGGATAACAGGCCATCAACTCCACTTTCTGTGAAGCGAGAAGCGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTGGTCTGTTGCAATCACTCAATCCCTCAACCCCAAAAGCAGGAAGTTCTGAGGGTAGTAGCGGTAGCAAATATTCATTTGGGTATATTTGGGGAAGCACCAAGGAGAGCCATAAGTGGAATACGAAAAAAATCCTTGCCTTGCGAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATGAAATTCTGGATGGAGAATTTGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCTCTGCTCCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATCGGCAGATTATACGCAAGTTATGGCCAATATTCGTCTTCCTGTTTGCTTCCATTCTCGTTCTTGAGTATTTTGCCATCTGGAAGAGTGCTTGGCCTCCAAATCATCCTGATGCAACTAATCCACGTTGCCATGATTGCTGGCAAAAGTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTAATGTTGATGATCCTCGAATGCTTATCAGCTATTTTATGGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCGGGCTTATCAACGTACCGTGAGATGATATCTCAACGTGAAAATACATTTGTATGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTTTACTGCTATTGCCATCTACTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACCATTGAGTATGACATCCTGCACCTTGGTTATCTCGCTTTTGCACTGGTTTTCTTTCGGGTTAGACTTCAAATACTAAAGAAAAGGAACAAGATGTTCAAGTACTTGCGCATATACAATTTTGCTCTCATTGTTCTATCTCTTGCATATCAATCTCCTTTTGTGGGTTGTTCTGGGAAGTGTGAGACCGTATTCGAGATGATTGGCTTTTATAAATATGACTATGGGTTTCAGATGACTGCAAGATCAGCTCTCGTTGAGATTATCATATTTATGCTGAAAAAAAAAGCTGCATGGAAAACTGCACAATTACAACATATTCGTGAATCTGAGGAGAAGAAACGCCAGCGTAACTTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCAGCTTCACAGCACGAACTCAGTTACTGACTGCGGTGACTCTTCTCCTGTCAGTGAAGGCCTAAGAAGGAGGAGAAGTACTTCTCTTAACTTGAACAATGATGCTTGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGAGACTTCTAGCAATAAATTGCATGAATCTCCTGCAACAGGGAATCTGGAAAACCTCACAGTTGGGGATTCTATGAAAAATTCAATGCAATCTATTCATTGTGAGATCACCGAAATTGAAGAGGACGTTGCTGATGGTACATTTTCTGATTCAGAGAAGAAAGAGAAGGGTAAAGGGAAATCAATGGATAACCCTCTAATATCAGCAGTTCATCTGATAGGTGATGGTGTTTCCCAGGTACAGTCCATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTGAATATTGACCAAGAATCCGATATCCATGAGCACTCTGCTGAGGATGGGGTATATGATGAGATGGAGAGCCAGAAAATAAAGTACGCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATATGAGTTCTGATGCCACAAGTCTGCAGTTAGGAAGGATCTTCCGTCACATATGGTCCCGAATGCGGTCCAATAATGATGTTGTCTGTTATTGTTGCTTTGTCATCGTCTTTTTGTGGAACTTCAGTTTGCTTTCCATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTCTGTGTTAATTCTGGTCCAAGTTACATATTCTGGGTTATCATGCTCATATACACAGAAGTTTATATTTTGCTTCTGTATCTATACCAGATTATCATCCAGCACTATGGTTCGCGTATTGCTTCAGACCTACTTCGTGAGTTGGGATTTCCTGCGCGTAAAATGTCTTCATTTGTTGTCAGTTCGTTGCCCCTATTTCTTGTGTACTTATTCACCCTTGTCCAGAGCTCTATAACCGCAAAGGATGGCGAGTGGATGTCTTCAACAGACTTCAACTTCTATAGGAGGAACGCTTTGCAGGGAAAAGAGGTTCCTGTCGGTTATAGCTGGAGTGAGAGGGCAAAGGACCCGCTGCACATAATTGTAAATGTCATGCAGTTGATATGTAGAAGCTTCTTTAGGTACTGGGTATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTGTTCAGGTTTCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCAGAAAGGATTGAGTCTGGAGTAAATCAATTGCTTAGAATCATCCATGATGAAAGATGCAAGGCAAAGGATCCTAAGCGATGCCGTTTTGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCGAGAAAATGGAAATGTGGCCTTGGTCGTTTTTGAGGTAGTATATGCCTCCCCTATAACAGACTGTGCTTCGGTAGAATGGTACAAGTCACTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCTTAAAGCACTGCATGCTGGCTTTGTTGAAGAAATAGGATTCCCATATCCTATACTCTCTGTTATCGGGGGAGGCAAAAGGGACATTGATCTGTTTGCCTATGTATTTGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTCCATTATAAAAAATAAAAGTGACTTTCTTGACGTGTATCAGCTTGAAGATCAATTTCCTAAAGAGTTCGTCTTCATCTTAATGATTATTTTTTTCTTGATTGTCCTTGATCGAATAATCTACCTCTGTTCATTTGCCACTGGAAAAGTGATTTACTACCTTTTTAACCTCATCCTCTTCACGTATTCAGTTACAAAGTATGCTTGGCATATGGAATCTTCCCGCCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAATCAATATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATTTCACGAATTAATTACTTAGGATATCGACTATATCGTGCTCTGCCATTCCTTTATGAATTGAGATGTGTACTTGACTGGTCATGTACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTCGTCAAATGTGATGCAGTCCTGAATAGAGCAACGCACAAACAAGGAGAAAAGCAAACACAAATGACCAAATGTTGCAATGGGATTTGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCCATTAAAGATGCCAGTGTTCAAGTGGATATCAAGACAATGAGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATTGATTGGGATAACGTCAACTCTGATGTTAATCTTGACCCTCAAGGTTATTTGGAGACATATAACAAGAAAGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCTAATGTCGTTCAGACCAGATTCATACAGTCCCTCAATTGGGAGAGCAACATGGCTATAACTTTTACTTGGGTACTTTCCAGGGAGAGACCCAAAGGCAAGGAAGTTGTGAAATATGAAAATACTCTTGGTTTTGAGGATCTTCCAAAACAATCAGATGTACAAAAAGTCCTTAATGGCTCTATCAACAGCTTTAGGATACACAATGTCTATCCAAGATACTTGCGTGTCACAGGTTCTGGTGATGTCAGGCCACTGGAACCAGGGGAAGCTTTCGTCACTGCGGACCTCGTTATAAATCGTGCTAGTTACCAGTGGTGGTGGTCCTTCAGTGACATCAATTCATCAGATGTCAACGGATGTGGCGGTTTGAGAGGACCCATGGCCATTATTATGTCTGAAGAAACGCCGCCACAGGGTATTCTTGGTGACACACTAAGCAAGTTCAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCCTATGAAAATCTCCCCTCCTGTGATAGGTTGTTGGCCATTTGTGAGGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGTATTGAAGAGATTCTTTACTGGACACTGGTGAAGATTTACAGATCACCACACATGCTGCTAGAATACACGAAACCTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0015075 GO:0015267 GO:0015318 GO:0016020 GO:0022803 GO:0022836 GO:0022838 GO:0022857 GO:0022890 GO:0034220 GO:0042391 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0065007 GO:0065008 GO:0071214 GO:0071260 GO:0071496 GO:0098655 GO:0104004

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Piezo_RRas_bdg

Protein Analysis

2371

Amino Acids

271.2

Weight (kDa)

8.22

Isoelectric Point (pI)

43.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIEZO	PF25288	402 - 531	1.3e-63	Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1	PF23188	1514 - 1632	3.5e-15	Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor	PF24874	1825 - 2062	1.2e-65	Piezo, THU9 and anchor domain
Piezo_cap	PF12166	2090 - 2370	1.8e-77	Piezo non-specific cation channel, cap domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006607)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48060 AT2G48060 AT2G48060
fragaria_vesca	FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180 FvH4_2g24180
malus_domestica	MD03G1271800.v1.1 MD11G1292400.v1.1
prunus_persica	Prupe.8G248000_v2.0.a1 Prupe.8G248000_v2.0.a1
pyrus_communis	pycom03g21640 pycom11g25880
rosa_chinensis	RchiOBHm_Chr6g0291991
rosa_laevigata	RLG00000012072
rosa_multiflora	Rmu_sc0008666.1_g000011
rosa_roxburghii	Rroxscaffold_7G00175510 Rroxscaffold_7G00178010
rosa_rugosa	Rorug06G0222200 Rorug06G0222300
rosa_samantha	Rh6AG336100 Rh6BG343500 Rh6CG349700 Rh6DG336500
rosa_wichuraiana	Rw6G029260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	3726, 5546, 6716
Acc16I	TGCGCA	1 cut(s)	3623
Acc36I	ACCTGC	1 cut(s)	2121
AccB7I	CCANNNNNTGG	3 cut(s)	219, 3478, 5126
AccI	GTMKAC	1 cut(s)	297
AccII	CGCG	2 cut(s)	4756, 4798
AccIII	TCCGGA	1 cut(s)	747
AclI	AACGTT	1 cut(s)	1344
AclWI	GGATC	8 cut(s)	1424, 2144, 3260, 3489, 4517, 5201, 5214, 6567
AcoI	YGGCCR	8 cut(s)	2398, 2720, 3086, 5117, 5523, 6804, 6907, 6987
AcsI	RAATTY	7 cut(s)	2165, 2462, 2927, 2941, 4147, 5850, 5895
AcuI	CTGAAG	8 cut(s)	510, 739, 2519, 4579, 4749, 6733, 6840, 6908
AdeI	CACNNNGTG	2 cut(s)	4646, 5789
AfeI	AGCGCT	1 cut(s)	1916
AflIII	ACRYGT	1 cut(s)	5571
AjiI	CACGTC	1 cut(s)	5572
AjuI	GAANNNNNNNTTGG	2 cut(s)	6531, 6563
AloI	GAACNNNNNNTCC	4 cut(s)	2930, 2962, 4935, 4967
Alw21I	GWGCWC	7 cut(s)	2647, 4386, 4871, 5114, 5938, 6284, 6932
AlwI	GGATC	8 cut(s)	1424, 2144, 3260, 3489, 4517, 5201, 5214, 6567
AlwNI	CAGNNNCTG	5 cut(s)	3231, 3938, 5366, 6659, 6873
Ama87I	CYCGRG	2 cut(s)	1242, 3346
Aor13HI	TCCGGA	1 cut(s)	747
Aor51HI	AGCGCT	1 cut(s)	1916
ApoI	RAATTY	7 cut(s)	2165, 2462, 2927, 2941, 4147, 5850, 5895
ArsI	GACNNNNNNTTYG	4 cut(s)	2039, 2071, 3398, 3430
AseI	ATTAAT	1 cut(s)	5907
Asp700I	GAANNNNTTC	5 cut(s)	2713, 2945, 3991, 4038, 7044
AspLEI	GCGC	4 cut(s)	1261, 1917, 3624, 4798
AsuNHI	GCTAGC	1 cut(s)	5228
AvaI	CYCGRG	2 cut(s)	1242, 3346
BaeI	ACNNNNGTAYC	2 cut(s)	6631, 6664
BalI	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
BamHI	GGATCC	1 cut(s)	5206
BanII	GRGCYC	2 cut(s)	4871, 6169
BarI	GAAGNNNNNNTAC	4 cut(s)	1458, 1490, 4792, 4824
BauI	CACGAG	1 cut(s)	1504
BbsI	GAAGAC	7 cut(s)	919, 1894, 3091, 3289, 4800, 4893, 5602
Bbv12I	GWGCWC	7 cut(s)	2647, 4386, 4871, 5114, 5938, 6284, 6932
BbvCI	CCTCAGC	1 cut(s)	4392
BceAI	ACGGC	1 cut(s)	5205
BciVI	GTATCC	3 cut(s)	799, 5124, 6612
BclI	TGATCA	1 cut(s)	3328
BfmI	CTRYAG	4 cut(s)	1966, 4463, 4496, 4921
BfoI	RGCGCY	2 cut(s)	1262, 1918
BfuAI	ACCTGC	1 cut(s)	2121
BfuI	GTATCC	3 cut(s)	799, 5124, 6612
BglII	AGATCT	1 cut(s)	2217
BmcAI	AGTACT	5 cut(s)	1171, 2575, 3239, 3617, 3991
BmeT110I	CYCGRG	2 cut(s)	1242, 3346
BmgBI	CACGTC	1 cut(s)	5572
BmrI	ACTGGG	1 cut(s)	5056
BmtI	GCTAGC	1 cut(s)	5232
BmuI	ACTGGG	1 cut(s)	5056
BpiI	GAAGAC	7 cut(s)	919, 1894, 3091, 3289, 4800, 4893, 5602
BpmI	CTGGAG	8 cut(s)	85, 832, 1414, 4990, 5175, 5343, 5793, 6041
Bpu10I	CCTNAGC	2 cut(s)	4392, 5211
BpuEI	CTTGAG	5 cut(s)	170, 1993, 2984, 3146, 6324
BsaAI	YACGTR	1 cut(s)	5721
BsaBI	GATNNNNATC	3 cut(s)	2028, 4736, 6327
BsaI	GGTCTC	7 cut(s)	515, 1184, 1490, 3399, 3692, 6502, 6884
BsaWI	WCCGGW	2 cut(s)	747, 784
BsaXI	ACNNNNNCTCC	4 cut(s)	2930, 2960, 3973, 4003
Bse3DI	GCAATG	8 cut(s)	1272, 1791, 2415, 2593, 3030, 3035, 6130, 6204
Bse8I	GATNNNNATC	3 cut(s)	2028, 4736, 6327
BseAI	TCCGGA	1 cut(s)	747
BseJI	GATNNNNATC	3 cut(s)	2028, 4736, 6327
BseMI	GCAATG	8 cut(s)	1272, 1791, 2415, 2593, 3030, 3035, 6130, 6204
BseMII	CTCAG	8 cut(s)	261, 1131, 1599, 1753, 2775, 3834, 3945, 4383
BseRI	GAGGAG	6 cut(s)	626, 1669, 1859, 2134, 3860, 3997
BseYI	CCCAGC	1 cut(s)	1985
BsgI	GTGCAG	4 cut(s)	1482, 3518, 3801, 4979
Bsh1236I	CGCG	2 cut(s)	4756, 4798
BsiHKAI	GWGCWC	7 cut(s)	2647, 4386, 4871, 5114, 5938, 6284, 6932
BsiHKCI	CYCGRG	2 cut(s)	1242, 3346
BsiSI	CCGG	2 cut(s)	748, 785
BslFI	GGGAC	5 cut(s)	1239, 4513, 5059, 5480, 6436
BsmFI	GGGAC	5 cut(s)	1239, 4513, 5059, 5480, 6436
BsmI	GAATGC	5 cut(s)	676, 878, 3016, 3279, 4539
Bso31I	GGTCTC	7 cut(s)	515, 1184, 1490, 3399, 3692, 6502, 6884
BsoBI	CYCGRG	2 cut(s)	1242, 3346
Bsp1286I	GDGCHC	8 cut(s)	2647, 4386, 4871, 5114, 5938, 6169, 6284, 6932
Bsp13I	TCCGGA	1 cut(s)	747
Bsp1407I	TGTACA	3 cut(s)	5242, 5981, 6580
Bsp19I	CCATGG	3 cut(s)	1558, 4608, 6801
BspCNI	CTCAG	8 cut(s)	262, 1130, 1598, 1752, 2776, 3835, 3944, 4384
BspEI	TCCGGA	1 cut(s)	747
BspFNI	CGCG	2 cut(s)	4756, 4798
BspMAI	CTGCAG	2 cut(s)	4467, 4500
BspMI	ACCTGC	1 cut(s)	2121
BspOI	GCTAGC	1 cut(s)	5232
BspPI	GGATC	8 cut(s)	1424, 2144, 3260, 3489, 4517, 5201, 5214, 6567
BspQI	GCTCTTC	1 cut(s)	2448
BspTNI	GGTCTC	7 cut(s)	515, 1184, 1490, 3399, 3692, 6502, 6884
BsrDI	GCAATG	8 cut(s)	1272, 1791, 2415, 2593, 3030, 3035, 6130, 6204
BsrGI	TGTACA	3 cut(s)	5242, 5981, 6580
BssNAI	GTATAC	1 cut(s)	298
BssSI	CACGAG	1 cut(s)	1504
BssT1I	CCWWGG	9 cut(s)	792, 1558, 2284, 2835, 3495, 3538, 4608, 5283, 6801
Bst1107I	GTATAC	1 cut(s)	298
Bst2BI	CACGAG	1 cut(s)	1504
BstAPI	GCANNNNNTGC	5 cut(s)	2999, 3041, 3240, 5399, 5789
BstAUI	TGTACA	3 cut(s)	5242, 5981, 6580
BstBAI	YACGTR	1 cut(s)	5721
BstDSI	CCRYGG	3 cut(s)	1558, 4608, 6801
BstEII	GGTNACC	1 cut(s)	6191
BstENI	CCTNNNNNAGG	4 cut(s)	1382, 1493, 4104, 6976
BstFNI	CGCG	2 cut(s)	4756, 4798
BstH2I	RGCGCY	2 cut(s)	1262, 1918
BstHHI	GCGC	4 cut(s)	1261, 1917, 3624, 4798
BstNSI	RCATGY	7 cut(s)	2529, 3433, 5243, 5402, 5771, 6035, 7090
BstPI	GGTNACC	1 cut(s)	6191
BstSFI	CTRYAG	4 cut(s)	1966, 4463, 4496, 4921
BstUI	CGCG	2 cut(s)	4756, 4798
BstV2I	GAAGAC	7 cut(s)	919, 1894, 3091, 3289, 4800, 4893, 5602
BstX2I	RGATCY	5 cut(s)	2217, 3481, 4509, 5206, 6559
BstXI	CCANNNNNNTGG	3 cut(s)	1438, 4748, 5771
BstYI	RGATCY	5 cut(s)	2217, 3481, 4509, 5206, 6559
BstZ17I	GTATAC	1 cut(s)	298
BsuI	GTATCC	3 cut(s)	799, 5124, 6612
BtgI	CCRYGG	3 cut(s)	1558, 4608, 6801
BtgZI	GCGATG	1 cut(s)	5230
BtrI	CACGTC	1 cut(s)	5572
BtsI	GCAGTG	4 cut(s)	5393, 5822, 6193, 6699
BveI	ACCTGC	1 cut(s)	2121
CaiI	CAGNNNCTG	5 cut(s)	3231, 3938, 5366, 6659, 6873
CfoI	GCGC	4 cut(s)	1261, 1917, 3624, 4798
CsiI	ACCWGGT	1 cut(s)	1285
CspCI	CAANNNNNGTGG	2 cut(s)	6763, 6798
DraIII	CACNNNGTG	2 cut(s)	4646, 5789
EaeI	YGGCCR	8 cut(s)	2398, 2720, 3086, 5117, 5523, 6804, 6907, 6987
Ecl136II	GAGCTC	1 cut(s)	4869
Eco130I	CCWWGG	9 cut(s)	792, 1558, 2284, 2835, 3495, 3538, 4608, 5283, 6801
Eco147I	AGGCCT	1 cut(s)	3972
Eco24I	GRGCYC	2 cut(s)	4871, 6169
Eco31I	GGTCTC	7 cut(s)	515, 1184, 1490, 3399, 3692, 6502, 6884
Eco32I	GATATC	4 cut(s)	3375, 4375, 5921, 6244
Eco47III	AGCGCT	1 cut(s)	1916
Eco53kI	GAGCTC	1 cut(s)	4869
Eco57I	CTGAAG	8 cut(s)	510, 739, 2519, 4579, 4749, 6733, 6840, 6908
Eco88I	CYCGRG	2 cut(s)	1242, 3346
Eco91I	GGTNACC	1 cut(s)	6191
EcoICRI	GAGCTC	1 cut(s)	4869
EcoNI	CCTNNNNNAGG	4 cut(s)	1382, 1493, 4104, 6976
EcoO109I	RGGNCCY	2 cut(s)	4987, 6797
EcoO65I	GGTNACC	1 cut(s)	6191
EcoRI	GAATTC	1 cut(s)	5850
EcoRV	GATATC	4 cut(s)	3375, 4375, 5921, 6244
EcoT14I	CCWWGG	9 cut(s)	792, 1558, 2284, 2835, 3495, 3538, 4608, 5283, 6801
EcoT22I	ATGCAT	2 cut(s)	880, 2245
EcoT38I	GRGCYC	2 cut(s)	4871, 6169
ErhI	CCWWGG	9 cut(s)	792, 1558, 2284, 2835, 3495, 3538, 4608, 5283, 6801
FalI	AAGNNNNNCTT	6 cut(s)	2459, 2491, 2986, 3018, 7032, 7064
FaqI	GGGAC	5 cut(s)	1239, 4513, 5059, 5480, 6436
FauI	CCCGC	2 cut(s)	4998, 5768
FauNDI	CATATG	5 cut(s)	775, 856, 1013, 4523, 5748
FbaI	TGATCA	1 cut(s)	3328
FblI	GTMKAC	1 cut(s)	297
FriOI	GRGCYC	2 cut(s)	4871, 6169
FspI	TGCGCA	1 cut(s)	3623
GlaI	GCGC	4 cut(s)	1260, 1916, 3623, 4797
GsaI	CCCAGC	1 cut(s)	1989
GsuI	CTGGAG	8 cut(s)	85, 832, 1414, 4990, 5175, 5343, 5793, 6041
HaeII	RGCGCY	2 cut(s)	1262, 1918
HapII	CCGG	2 cut(s)	748, 785
HhaI	GCGC	4 cut(s)	1261, 1917, 3624, 4798
Hin6I	GCGC	4 cut(s)	1259, 1915, 3622, 4796
HinP1I	GCGC	4 cut(s)	1259, 1915, 3622, 4796
HincII	GTYRAC	7 cut(s)	732, 1348, 3213, 4333, 6267, 6316, 6775
HindII	GTYRAC	7 cut(s)	732, 1348, 3213, 4333, 6267, 6316, 6775
HindIII	AAGCTT	7 cut(s)	556, 2049, 2316, 2995, 5032, 6037, 6690
HpaI	GTTAAC	2 cut(s)	1348, 4333
HpaII	CCGG	2 cut(s)	748, 785
HpyCH4IV	ACGT	8 cut(s)	1344, 3187, 3359, 3382, 4194, 5571, 5720, 6312
HpySE526I	ACGT	8 cut(s)	1344, 3187, 3359, 3382, 4194, 5571, 5720, 6312
HspAI	GCGC	4 cut(s)	1259, 1915, 3622, 4796
Kpn2I	TCCGGA	1 cut(s)	747
Ksp22I	TGATCA	1 cut(s)	3328
KspAI	GTTAAC	2 cut(s)	1348, 4333
LguI	GCTCTTC	1 cut(s)	2448
MabI	ACCWGGT	1 cut(s)	1285
MaeII	ACGT	8 cut(s)	1344, 3187, 3359, 3382, 4194, 5571, 5720, 6312
MfeI	CAATTG	2 cut(s)	5165, 6457
MflI	RGATCY	5 cut(s)	2217, 3481, 4509, 5206, 6559
MhlI	GDGCHC	8 cut(s)	2647, 4386, 4871, 5114, 5938, 6169, 6284, 6932
MlsI	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
MluNI	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
MlyI	GAGTC	7 cut(s)	252, 281, 2036, 3944, 5081, 5159, 5269
MmeI	TCCRAC	1 cut(s)	2538
Mox20I	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
Mph1103I	ATGCAT	2 cut(s)	880, 2245
MroI	TCCGGA	1 cut(s)	747
MroXI	GAANNNNTTC	5 cut(s)	2713, 2945, 3991, 4038, 7044
MscI	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
Msp20I	TGGCCA	7 cut(s)	2400, 2722, 3088, 5119, 5525, 6806, 6989
MspA1I	CMGCKG	4 cut(s)	50, 2553, 4993, 5366
MspI	CCGG	2 cut(s)	748, 785
MunI	CAATTG	2 cut(s)	5165, 6457
Mva1269I	GAATGC	5 cut(s)	676, 878, 3016, 3279, 4539
MvnI	CGCG	2 cut(s)	4756, 4798
NcoI	CCATGG	3 cut(s)	1558, 4608, 6801
NdeI	CATATG	5 cut(s)	775, 856, 1013, 4523, 5748
NheI	GCTAGC	1 cut(s)	5228
NmeAIII	GCCGAG	1 cut(s)	2595
NmuCI	GTSAC	8 cut(s)	3947, 4517, 5349, 5558, 6649, 6697, 6752, 6848
NsbI	TGCGCA	1 cut(s)	3623
NsiI	ATGCAT	2 cut(s)	880, 2245
NspI	RCATGY	7 cut(s)	2529, 3433, 5243, 5402, 5771, 6035, 7090
PaeI	GCATGC	2 cut(s)	2529, 5402
PceI	AGGCCT	1 cut(s)	3972
PciSI	GCTCTTC	1 cut(s)	2448
PcsI	WCGNNNNNNNCGW	1 cut(s)	5929
PctI	GAATGC	5 cut(s)	676, 878, 3016, 3279, 4539
PdmI	GAANNNNTTC	5 cut(s)	2713, 2945, 3991, 4038, 7044
PflMI	CCANNNNNTGG	3 cut(s)	219, 3478, 5126
PfoI	TCCNGGA	1 cut(s)	2416
PleI	GAGTC	7 cut(s)	252, 280, 2036, 3944, 5080, 5158, 5268
PpsI	GAGTC	7 cut(s)	252, 280, 2036, 3944, 5080, 5158, 5268
Ppu21I	YACGTR	1 cut(s)	5721
PpuMI	RGGWCCY	2 cut(s)	4987, 6797
PshBI	ATTAAT	1 cut(s)	5907
PsiI	TTATAA	3 cut(s)	3726, 5546, 6716
Psp124BI	GAGCTC	1 cut(s)	4871
Psp1406I	AACGTT	1 cut(s)	1344
Psp5II	RGGWCCY	2 cut(s)	4987, 6797
PspEI	GGTNACC	1 cut(s)	6191
PspFI	CCCAGC	1 cut(s)	1985
PspPPI	RGGWCCY	2 cut(s)	4987, 6797
PstI	CTGCAG	2 cut(s)	4467, 4500
PstNI	CAGNNNCTG	5 cut(s)	3231, 3938, 5366, 6659, 6873
PsuI	RGATCY	5 cut(s)	2217, 3481, 4509, 5206, 6559
PvuII	CAGCTG	2 cut(s)	50, 5366
SacI	GAGCTC	1 cut(s)	4871
SapI	GCTCTTC	1 cut(s)	2448
ScaI	AGTACT	5 cut(s)	1171, 2575, 3239, 3617, 3991
SchI	GAGTC	7 cut(s)	252, 281, 2036, 3944, 5081, 5159, 5269
SduI	GDGCHC	8 cut(s)	2647, 4386, 4871, 5114, 5938, 6169, 6284, 6932
SexAI	ACCWGGT	1 cut(s)	1285
SfcI	CTRYAG	4 cut(s)	1966, 4463, 4496, 4921
SmlI	CTYRAG	5 cut(s)	185, 1972, 2963, 3125, 6339
SmoI	CTYRAG	5 cut(s)	185, 1972, 2963, 3125, 6339
SphI	GCATGC	2 cut(s)	2529, 5402
SseBI	AGGCCT	1 cut(s)	3972
SspI	AATATT	6 cut(s)	1266, 2437, 2638, 2807, 3093, 4357
SstI	GAGCTC	1 cut(s)	4871
StuI	AGGCCT	1 cut(s)	3972
StyI	CCWWGG	9 cut(s)	792, 1558, 2284, 2835, 3495, 3538, 4608, 5283, 6801
TaiI	ACGT	8 cut(s)	1347, 3190, 3362, 3385, 4197, 5574, 5723, 6315
TaqI	TCGA	7 cut(s)	216, 904, 3271, 3708, 5263, 5650, 5923
TaqII	GACCGA	1 cut(s)	557
TauI	GCSGC	3 cut(s)	2705, 6832, 6912
TseFI	GTSAC	8 cut(s)	3947, 4517, 5349, 5558, 6649, 6697, 6752, 6848
Tsp45I	GTSAC	8 cut(s)	3947, 4517, 5349, 5558, 6649, 6697, 6752, 6848
TspGWI	ACGGA	6 cut(s)	668, 884, 2447, 4505, 5777, 6792
Van91I	CCANNNNNTGG	3 cut(s)	219, 3478, 5126
VspI	ATTAAT	1 cut(s)	5907
XagI	CCTNNNNNAGG	4 cut(s)	1382, 1493, 4104, 6976
XapI	RAATTY	7 cut(s)	2165, 2462, 2927, 2941, 4147, 5850, 5895
XbaI	TCTAGA	3 cut(s)	607, 698, 2485
XceI	RCATGY	7 cut(s)	2529, 3433, 5243, 5402, 5771, 6035, 7090
XcmI	CCANNNNNNNNNTGG	2 cut(s)	4615, 6235
XmiI	GTMKAC	1 cut(s)	297
XmnI	GAANNNNTTC	5 cut(s)	2713, 2945, 3991, 4038, 7044
ZrmI	AGTACT	5 cut(s)	1171, 2575, 3239, 3617, 3991
Zsp2I	ATGCAT	2 cut(s)	880, 2245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.