FvH4_2g24180

Piezo non-specific cation channel, R-Ras-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006607
Physical Location & Seq
Forward (+)
19731914 .. 19746687
14774 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24180.t2

Sequence Viewer

Length: 7425 bp
ATGGGCAGATTTGTTGGTACCTTTCTGTTGCCTCTGCTGCTGCTTGCAGCTGCTTGCATTCACTGGAGCTTGATCTCTCTTGTTGATTTGATAGCTTTTCTTCTTATTGTATATCATGCCCCGAAAATAGGATTTCATTTTGGAAGGAGGCTTTTATTTTTGTGGTTCATCATCATATTTTCTTTATTTGTTATTTTCTCTGAAGTTCTATATCTGGTCATATGGGCCATCGAGGGAAATATATGGAGTATAGCACAAGCGTGGTGGGGAAAGCTCATTGGATTCTTGATAGCTGAGTCATGGAAATCACCATCTGTGGTATACTTTTTGGTGCTTCAACTCTCCATAGTTGCTGTTTCGTTAGTTGATTTGTATGGGAACAGATTTGGTCTTTCTTCATCATATGATTCATGTTGGGGACAATTCTCATCAACTGTTGATCGCTTAGTGTGTTCTCATCTAAGGGTTGCTTCTTGCTTGCTGTTGCCTGCTATTCAACTGGTTGTTGGAATCAGCCATCCATCATGGGTTTCTCTACCATTTTTTATTGGGAGCTGTGTTGGTCTTGTGGATTGGTCCTTGACAAGCAATTTTTTAGGACTTTTCAGGTGGTGGAGGCCCTTTCACTTGTATGCAGGCTTCAACATTGTCTTGCTTTATCTGTATCAGCTCCCTGTGGAGTTTCCAGTTATGTTACAATGGGTAGCTGATTTCATTGGTCTCTTCAAAATAACTTCAGGTTCCCAATGGACCGAAGTTTGTTCAAGCTTCTCTCTTGTACTTTTCTACATAATGTTAGCCTGTGTTAAATGTGATCTGGAGGAGATGGATTTTATCTTGTCTATGAAAGAAAATAACTTGATGGAGCATCTTCTTCCCTCAAAGCATTCATTTTTCATTCGTCAATCTAGATCTGGTGCAAGGCATACAAATGTTTTGTTGACAGGGGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTATCCTTGGTTGCTCTTTCCTTTTGGAGTTTTCATTTTGCAAGTGTTTGTGCTTTTGCGCTACTTGCATATGTTGGCTACGTCATATATGCATTCCCTTCCTTATTCCGTTTGCATCGATTGAATGGGCTGCTTCTTGTCTTCATTCTCTTGTGGGCTGTTAGCACATATATCTTCAATGTAGCCTTCACATTCTTGAATTGGAAAATTGGGAAGAACATGGACATATGGGAGTTGGTTGGCTTGTGGCATTATCCCATACCTGGATTCTTTTTGCTTGCACAATTTTGTCTTGGAATATTGGTTGCGTTGGGTAATCTTGTGAACAACTCGGTTTTCCTTTGCTTGTCTGATGAGGAAGGGCGATCATCAAATGAAAATAGTATTATCGAAGGCGAGGGAGAGACCAAAGTTTTGATTGTTGCCACAATTGCTTGGGGACTGCGCAAATGCTCTCGGCCTATCATGCTGGTGCTGATATTTCTCATTGCCATGAAACCTGGTTTCATCCACGCAGTGTATGTGATGTTCTTTTTGATCTATCTTTTGAGCCACAACATTAACAGAAAATTACGCCAGTCTTTGATACTCCTATGCGAGGTTCACTTTGCACTATTGTATATCATTCAGATCAATCCGATCTCCAATAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCCTATTGCAGTTAGGTCTCCTTGAAAAGGAAAGCTCATGGGATTTCTTGGAAATAGCTTTGCTTGCTTGCTTCTGTGCAATTCATAACCATGGTTTCGAAATGCTTTTTTCGTTCTCAGCGATTGTGCAACATACCCCTAGCCTTCCTTTTGGATTTAGCATTTTGAAAGCTGGTCTGAACAAATCCGTACTGTTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTGTTCCTCAGTGCAATCGGACAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAGACCGAATTATATCTCATTTGGGTACATATTTCTCCTTCTCGTTTGGATAATTGGAAGACAACTTGTTGAGAGAACAAAAAAGCGCCTGTGGTTCCCATTAAAAGCATATTCAATTATGGTATTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGCTTGGTTGTCCAGGTTGATAGACCTTAAATTTTATCTGGGGTATGAGTCAAAAGCTTCATGTTTGGAAAATGTTTGGGAGTCCCTAGCAGTCTTGATTGTGATGCAACTTTACAGCTATGAGAGGAGGCAGAGTAGGTACAACAGGTCAGATGGTCCTGATTTGACAGAGTTTGGAGTGCTTGGGTTCGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTTTTCTATGCATCTTTATCTCCAATTAGTGCATCTAGCTTTTTGTATCTCCTTGGCCTTGTTATCTGTTCAACTTTACCTAAAGCTTCACGAATTCCGTCTAAATCATTCTTGGCATACACAGGATTTCTAGTAACAGCTGAGTATCTTTTTCAGATGTTGGGTAGCCAGGCAGCAATGTTTCCTGGACAAAAGCACTATAATATTTCTCTTCTTTTGGGTTTCCGTGAGTTTAAACCTGGTTTTTGGGGTCTAGAATCTGGATTGAGAGGAAAAGTTCTTGTGATTGCTGCATGCACCCTTCAGTATAATGTTTTCCACTGGTTGGAGAGGATGCCAAGTACTATTCTAAGCAAAGGAATGGGAGAGCCTTGTCCACTGTTTCTGTCAGCAGAAGATACAAATATCAGTGCTACCATCCCTAGTGAGGATAACAGGCCATCAACCTCATTTTCTGTGAAGCAAGAAGGGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTAGTCTGTTGCATTCACATAATCCCTCATCCCCAAAAGCAGGAACTTCTAAGGGTAGTAGCAGTGGCAAATATTCATTTGGATACATTTGGGGAAGCACCAAGGAGAGTCATAAGTGGAACAAGAAAAGGATCCTTGCCTTGCAAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATTAAATTCTGGTTGGAGAATATGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCCCTACTTCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATCGGCAGATTATACGTAAGTTATGGCCAACGTTCGTCTTCCTGTTTGCGTCCATTCTCATTCTTGAGTACTTTGCCATCTGGAAGAGTACATGGCCTCCAAATCATCCTGATGCAACTAATCCATGTTGCCATGATTGCTGGAATAACTCAACCATGTATTTCAGTTACTGCATGTACTGCTGGTTAGGACTTAATGTTGATGATCCCAGAATGCTTATAAGCTATTTTATAGTCTTCATGCTTGCTTGTTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCAGGCTCATCAACGTACCGTGAGATGATATCTCAATGTAAAAATACATTTGTGTGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTTTACTGCTATTGCCATCTACTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACTATTGAGTATGACATTCTGCACCTTGGTTATCTTGCTTTTGCGCTGGTTTTCTTTCGGGTTAGACTTGAAATACTAAAGAAAAGGAACAAGATGTTCAAGTACTTGCGCATTTATAATTTTGCTCTCATTGTTCTCTCTCTTGCATATCAATCACCTTTTGTGGGTTGTTCTGGGAAGTGTGAGAACGTAGATTACATGTTTGAGATGATTGGCTTTTATAAATATGACTATGGGTTTAAGATAACTGCAAGATCAGCTCTCGTGGAAATTATCATATTTATGCTGGTATCACTTCAGTCATATATGTTTTCCTCTAAAGAGTTTGATCATGTGTCTCGATATCTTGAAGCCGAACAAATTGGTATGATTGTGCGTGAACAAGAAAAAAAAGCTGCATGGAAAACTGCACAGTTGCAACATATTCGTGAATCTGAGGAGAAGAAACACCAGCGTAACCTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCAGCTTCACAGCATGAACTCGGTTACAAACTGCGGTGACTCTCCTGTCAGTGAAGGCCTAAGAAGGAGGAGAAGTACTTCTCTTAACTTGAATAATGATGCTGGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGACACTTCTAACATTGAATTGCATGATTCTCCTGCAACAGGGAATCTGGAAAACCTTGTTGTGGATTCTATGAGAAATTCAATGCAGTCAAGTCATTGTGAGATCACTGAAATTGAAGAGGACGTTGCTGATGGTACAGCTTTTGATTCAGAGAAGAAAGAGAAGGATAAAGGAAAATCAAAGGATAACCCACTAATATCAGCAGTTCATCTGATAGGTGATGGTGTTTCCCAGGTACAGTCCATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTAAATATTGACCAAGAGTCTGATATCCATGAGCACTCTCCTGAGGATGGGGTATATGATGAGATGGAGAGCCAGAAAACAAAGTATTCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATATGAGTTCAGATGCCACAAGTCTGCAGTTAGGAAGGATCTTCCGTCACATATGGTCCCGCATGCGGTCCAATAATGATGTCGTCTGTTATTGTTGCTTTGTCATCGTCTTTTTGTGGAACTTCAGTTTGCTTTCTATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTGTGTGTCAATTCTGGTCCAAGTTACATATTCTGGGTTGTCATGCTCATATACACAGAGGTCTATATTTTGCTTCTGTATCTATACCAGATTATTATCCAGCACTATGGCTTAGGTATTGCTTCAGAGCTACTGCATGAGTTGGGATTTCCTGGGCATAAACTCCCGTCTTCTTTTGTTGTCGGTTCTTTTCCCATTTTTCTTGTATACTTATTCACCCTCATCCAGAGCTCTATAACTGCAAAGGATGGCGAGTGGATGTCTTCTACAGACGTCAACCTCTATAGGAGGAATGCTTTCCATGGAAAAGAGGTTCCTGTTGGTTATAGCAGGACTGATAGGGCAAAGGACCTGCAGCACATACTTGAAAACTTCATAAAGTTGATATTTAGAAGCTTCTATAGGTACTGGGGATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTCTTCAGGTATCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCGGAAAGGATTGAGTCTGGAGTAAACCAGTTGCTTAGACTCATCCATGATGAAAGATGTAAGGCAAAGGATCCTAAGCAATGCCCTTTGGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCAAGAAAATGCAAATGTGGCCTTGGTCGTTTTTGAGGTGGTATATGCCTCTCCCATAACAGACTGTGCTTCGGCAGAATGGTACAAGTCGCTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCATAATGCCCTGCATGCTGGCTATGTTGAAGAAATAGGATTCCCATACCCTATACTCTCTGTTATCGGGGGAGGCAAAAAGGACATTGATCTTTATGCCTATGTATTTGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTATGTTATAAAAAATAAAAGTGACTTTCTTGACGTATACCAGCTTGAAGATCAATTTCCTAAAGAGTTTGTGTTCATCTTAATGATTATCTTTTTCTTGATTGTGCTTGACCGAATAATCTACCTCTGTTCATTTGCCACTGGAAAAGTGATATACTACCTTTTCAACCTCATCCTCTTCACATATTCAGTTACAAAGTATGCTTGGTATATGGAGCCTTCCCACCATGCCGGAGAATTAGCACTTCGTGCTATATTTCTTGCAAAATCAGTATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATTTCACGAATTAATTACTTAGGATATCGACTATATCGTGCTCTGCCATTCCTTTATGAATTGAGATGTGCACTTGACTGGTCATGCACAACTACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTTGTCAAATGTGATGCAGTCCTGAATAGAGCAACGCACAAACAAGGAGAAAAGCAAACACAAATGACCAAATGTTGCAATGGGATATGTCTGTTTTTCATATTGATTTGTGTCATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCTATTAAAGATGCCAGTGTTCAAGTTGATATTAAGACAACGGGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATCGATTGGGATGATGTTAATTCTAATGTTAATCTTGATCCTCAAGGTTATTTGGAGCCATATAATAAGAAGGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCCGATGTCGTGCAGACCAGATTCATACGGTCCCTCGATTGGGAGAGCAACATGGCTATAAGATTTACTTGGGAACTTTCCAGGGAGAGACCCAAAGGCAAGGAAGTTGTGAAATATTACAGTTATCCTGGTTTTGAGGATCTTCCAGAACAATCAGATGTACAAAAGGTCCTGAATGGCTCTACCAACAGCTTCAGGATACACAATGTCTATCCAAGATACTTGCGTGTAACTGGTTCTGGTGATGTCAGGCCACTGGAAACAGGGGAAATTTCCGTCACTGCAGACCTCGTTATAAATCGTGCTAGTTACCCATGGTGGTGGTCCTTCCTTGACATCAATTCATCAGATGTCAATGGATGCGGTGGTTTGAGAGGACCCATGGCCATTATTATGTCTGAAGAAACACCACCACAGGGTATTCTCGGCGACACGCTAAGCAAGTTCAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCTTATGAAAATCTTCCCTCCTGCGATAGGTTGTTGGCCATATGTGAGGATATTTATGCTGCCAGAGCTGAGGGTGAGCTTGGTATTGAAGAGATCCTTTACTGGACACTGGTGAAGATTTATAGATCACCACACATGCTTCTAGAGTACACGAAACCCGACTAG

Protein Analysis

2475

Amino Acids

283.22

Weight (kDa)

7.37

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIEZO PF25288 472 - 601 1.2e-63 Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1 PF23188 1617 - 1686 9.4e-15 Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor PF24874 1929 - 2165 6.3e-66 Piezo, THU9 and anchor domain
Piezo_cap PF12166 2193 - 2330 8.3e-22 Piezo non-specific cation channel, cap domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 3491, 3840, 3945, 5858, 7025
AasI GACNNNNNNGTC 1 cut(s) 4271
AatII GACGTC 1 cut(s) 5226
Acc16I TGCGCA 2 cut(s) 1442, 3833
Acc36I ACCTGC 2 cut(s) 4191, 5310
Acc65I GGTACC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 17
AccB7I CCANNNNNTGG 3 cut(s) 2767, 3688, 5438
AccI GTMKAC 3 cut(s) 321, 5157, 5886
AccIII TCCGGA 1 cut(s) 957
AciI CCGC 6 cut(s) 2401, 4260, 4840, 4846, 7092, 7219
AclI AACGTT 1 cut(s) 3302
AcoI YGGCCR 7 cut(s) 2930, 3296, 5429, 5835, 7113, 7216, 7296
AcsI RAATTY 7 cut(s) 2234, 2535, 3137, 4456, 6159, 6204, 6999
AcyI GRCGYC 1 cut(s) 5223
AdeI CACNNNGTG 3 cut(s) 1513, 4955, 6098
AflIII ACRYGT 1 cut(s) 3921
AhdI GACNNNNNGTC 1 cut(s) 4675
AleI CACNNNNGTG 1 cut(s) 259
AloI GAACNNNNNNTCC 4 cut(s) 3140, 3172, 5247, 5279
Alw21I GWGCWC 7 cut(s) 4695, 5183, 5426, 6247, 6277, 6593, 7241
Alw26I GTCTC 9 cut(s) 725, 1394, 1700, 2035, 3609, 4065, 4374, 6811, 7193
Alw44I GTGCAC 1 cut(s) 6273
AlwNI CAGNNNCTG 3 cut(s) 3441, 5678, 7182
Aor13HI TCCGGA 1 cut(s) 957
ApaLI GTGCAC 1 cut(s) 6273
ApoI RAATTY 7 cut(s) 2234, 2535, 3137, 4456, 6159, 6204, 6999
ArsI GACNNNNNNTTYG 4 cut(s) 1393, 1425, 3608, 3640
AseI ATTAAT 1 cut(s) 6216
Asp700I GAANNNNTTC 6 cut(s) 2923, 3155, 4303, 4350, 5246, 7272
Asp718I GGTACC 1 cut(s) 17
AspLEI GCGC 5 cut(s) 1057, 1443, 2127, 3769, 3834
AsuHPI GGTGA 9 cut(s) 300, 3870, 4274, 4610, 5158, 6983, 7346, 7380, 7384
AsuII TTCGAA 1 cut(s) 1776
AsuNHI GCTAGC 1 cut(s) 5540
AxyI CCTNAGG 1 cut(s) 4701
BaeGI GKGCMC 1 cut(s) 6277
BaeI ACNNNNGTAYC 2 cut(s) 6940, 6973
BalI TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
BamHI GGATCC 2 cut(s) 3075, 5518
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 2 cut(s) 5183, 6478
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BauI CACGAG 1 cut(s) 3986
BbsI GAAGAC 6 cut(s) 1129, 2104, 3301, 3499, 5112, 5205
Bbv12I GWGCWC 7 cut(s) 4695, 5183, 5426, 6247, 6277, 6593, 7241
BbvCI CCTCAGC 1 cut(s) 7329
BcgI CGANNNNNNTGC 2 cut(s) 4130, 4164
BciVI GTATCC 4 cut(s) 1009, 3020, 5436, 6921
BclI TGATCA 2 cut(s) 3538, 4051
BcoDI GTCTC 9 cut(s) 725, 1394, 1700, 2035, 3609, 4065, 4374, 6811, 7193
BfmI CTRYAG 8 cut(s) 2176, 4772, 4805, 5217, 5233, 5303, 5350, 7011
BfoI RGCGCY 1 cut(s) 2128
BfuAI ACCTGC 2 cut(s) 4191, 5310
BfuI GTATCC 4 cut(s) 1009, 3020, 5436, 6921
BglII AGATCT 2 cut(s) 911, 2427
BlpI GCTNAGC 1 cut(s) 7166
BmcAI AGTACT 4 cut(s) 2785, 3341, 3827, 4303
BmeRI GACNNNNNGTC 1 cut(s) 4675
BmrI ACTGGG 1 cut(s) 5368
BmtI GCTAGC 1 cut(s) 5544
BmuI ACTGGG 1 cut(s) 5368
BpiI GAAGAC 6 cut(s) 1129, 2104, 3301, 3499, 5112, 5205
BpmI CTGGAG 5 cut(s) 85, 839, 5487, 5655, 6350
Bpu10I CCTNAGC 3 cut(s) 5062, 5523, 7329
Bpu1102I GCTNAGC 1 cut(s) 7166
Bpu14I TTCGAA 1 cut(s) 1776
BpuEI CTTGAG 4 cut(s) 2203, 3194, 3356, 6633
Bsa29I ATCGAT 2 cut(s) 1114, 6609
BsaAI YACGTR 1 cut(s) 3287
BsaBI GATNNNNATC 1 cut(s) 5045
BsaHI GRCGYC 1 cut(s) 5223
BsaI GGTCTC 7 cut(s) 725, 1394, 1700, 2035, 3609, 6811, 7193
BsaWI WCCGGW 2 cut(s) 957, 994
BsaXI ACNNNNNCTCC 8 cut(s) 2323, 2353, 3140, 3170, 3352, 3382, 4285, 4315
Bse21I CCTNAGG 1 cut(s) 4701
Bse3DI GCAATG 8 cut(s) 1482, 2001, 2625, 3240, 3245, 5534, 6439, 6513
Bse8I GATNNNNATC 1 cut(s) 5045
BseAI TCCGGA 1 cut(s) 957
BseCI ATCGAT 2 cut(s) 1114, 6609
BseJI GATNNNNATC 1 cut(s) 5045
BseMI GCAATG 8 cut(s) 1482, 2001, 2625, 3240, 3245, 5534, 6439, 6513
BseMII CTCAG 8 cut(s) 285, 1809, 1963, 2574, 3570, 4149, 4692, 7320
BseRI GAGGAG 5 cut(s) 836, 1879, 2344, 4175, 4309
BseSI GKGCMC 1 cut(s) 6277
BseYI CCCAGC 2 cut(s) 2195, 4328
BsgI GTGCAG 4 cut(s) 1692, 3728, 4116, 6761
BshNI GGYRCC 1 cut(s) 17
BshVI ATCGAT 2 cut(s) 1114, 6609
BsiHKAI GWGCWC 7 cut(s) 4695, 5183, 5426, 6247, 6277, 6593, 7241
BsiSI CCGG 4 cut(s) 958, 995, 5450, 6081
BslFI GGGAC 7 cut(s) 432, 1449, 2272, 4345, 4822, 5371, 6745
BsmAI GTCTC 9 cut(s) 725, 1394, 1700, 2035, 3609, 4065, 4374, 6811, 7193
BsmFI GGGAC 7 cut(s) 432, 1449, 2272, 4345, 4822, 5371, 6745
BsmI GAATGC 7 cut(s) 57, 886, 1088, 2956, 3226, 3489, 5248
Bso31I GGTCTC 7 cut(s) 725, 1394, 1700, 2035, 3609, 6811, 7193
Bsp119I TTCGAA 1 cut(s) 1776
Bsp1286I GDGCHC 8 cut(s) 4695, 5183, 5426, 6247, 6277, 6478, 6593, 7241
Bsp13I TCCGGA 1 cut(s) 957
Bsp1407I TGTACA 2 cut(s) 5554, 6889
Bsp1720I GCTNAGC 1 cut(s) 7166
Bsp19I CCATGG 4 cut(s) 1768, 5251, 7043, 7110
BspACI CCGC 6 cut(s) 2401, 4260, 4840, 4846, 7092, 7219
BspCNI CTCAG 8 cut(s) 286, 1808, 1962, 2575, 3569, 4150, 4693, 7321
BspDI ATCGAT 2 cut(s) 1114, 6609
BspEI TCCGGA 1 cut(s) 957
BspMAI CTGCAG 4 cut(s) 4776, 4809, 5307, 7015
BspMI ACCTGC 2 cut(s) 4191, 5310
BspOI GCTAGC 1 cut(s) 5544
BspT104I TTCGAA 1 cut(s) 1776
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 7 cut(s) 725, 1394, 1700, 2035, 3609, 6811, 7193
BsrDI GCAATG 8 cut(s) 1482, 2001, 2625, 3240, 3245, 5534, 6439, 6513
BsrGI TGTACA 2 cut(s) 5554, 6889
BssNAI GTATAC 3 cut(s) 322, 5158, 5887
BssNI GRCGYC 1 cut(s) 5223
BssSI CACGAG 1 cut(s) 3986
Bst1107I GTATAC 3 cut(s) 322, 5158, 5887
Bst2BI CACGAG 1 cut(s) 3986
Bst6I CTCTTC 6 cut(s) 728, 2658, 3350, 4491, 6032, 7344
BstACI GRCGYC 1 cut(s) 5223
BstAPI GCANNNNNTGC 4 cut(s) 3209, 3251, 3450, 6098
BstAUI TGTACA 2 cut(s) 5554, 6889
BstBAI YACGTR 1 cut(s) 3287
BstBI TTCGAA 1 cut(s) 1776
BstDSI CCRYGG 4 cut(s) 1768, 5251, 7043, 7110
BstEII GGTNACC 1 cut(s) 6500
BstENI CCTNNNNNAGG 4 cut(s) 1592, 1703, 4416, 7285
BstH2I RGCGCY 1 cut(s) 2128
BstHHI GCGC 5 cut(s) 1057, 1443, 2127, 3769, 3834
BstMAI GTCTC 9 cut(s) 725, 1394, 1700, 2035, 3609, 4065, 4374, 6811, 7193
BstNSI RCATGY 9 cut(s) 2739, 3448, 3643, 3925, 4846, 5555, 5714, 6344, 7399
BstPI GGTNACC 1 cut(s) 6500
BstSFI CTRYAG 8 cut(s) 2176, 4772, 4805, 5217, 5233, 5303, 5350, 7011
BstSLI GKGCMC 1 cut(s) 6277
BstSNI TACGTA 1 cut(s) 3287
BstV2I GAAGAC 6 cut(s) 1129, 2104, 3301, 3499, 5112, 5205
BstX2I RGATCY 8 cut(s) 911, 2427, 3075, 3691, 4818, 5518, 6868, 7353
BstXI CCANNNNNNTGG 3 cut(s) 1648, 5057, 7050
BstYI RGATCY 8 cut(s) 911, 2427, 3075, 3691, 4818, 5518, 6868, 7353
BstZ17I GTATAC 3 cut(s) 322, 5158, 5887
Bsu15I ATCGAT 2 cut(s) 1114, 6609
Bsu36I CCTNAGG 1 cut(s) 4701
BsuI GTATCC 4 cut(s) 1009, 3020, 5436, 6921
BsuTUI ATCGAT 2 cut(s) 1114, 6609
BtgI CCRYGG 4 cut(s) 1768, 5251, 7043, 7110
BtsI GCAGTG 5 cut(s) 1518, 3013, 6131, 6502, 7008
BveI ACCTGC 2 cut(s) 4191, 5310
CaiI CAGNNNCTG 3 cut(s) 3441, 5678, 7182
CfoI GCGC 5 cut(s) 1057, 1443, 2127, 3769, 3834
ClaI ATCGAT 2 cut(s) 1114, 6609
CseI GACGC 1 cut(s) 3309
CsiI ACCWGGT 2 cut(s) 1495, 2680
CspCI CAANNNNNGTGG 2 cut(s) 245, 280
DraI TTTAAA 1 cut(s) 2677
DraIII CACNNNGTG 3 cut(s) 1513, 4955, 6098
DrdI GACNNNNNNGTC 1 cut(s) 4271
DriI GACNNNNNGTC 1 cut(s) 4675
DseDI GACNNNNNNGTC 1 cut(s) 4271
EaeI YGGCCR 7 cut(s) 2930, 3296, 5429, 5835, 7113, 7216, 7296
Eam1104I CTCTTC 6 cut(s) 728, 2658, 3350, 4491, 6032, 7344
Eam1105I GACNNNNNGTC 1 cut(s) 4675
EarI CTCTTC 6 cut(s) 728, 2658, 3350, 4491, 6032, 7344
Ecl136II GAGCTC 1 cut(s) 5181
Eco105I TACGTA 1 cut(s) 3287
Eco147I AGGCCT 1 cut(s) 4284
Eco24I GRGCYC 2 cut(s) 5183, 6478
Eco31I GGTCTC 7 cut(s) 725, 1394, 1700, 2035, 3609, 6811, 7193
Eco32I GATATC 4 cut(s) 3585, 4067, 4684, 6230
Eco53kI GAGCTC 1 cut(s) 5181
Eco81I CCTNAGG 1 cut(s) 4701
Eco91I GGTNACC 1 cut(s) 6500
EcoICRI GAGCTC 1 cut(s) 5181
EcoNI CCTNNNNNAGG 4 cut(s) 1592, 1703, 4416, 7285
EcoO109I RGGNCCY 5 cut(s) 618, 4332, 5299, 6898, 7106
EcoO65I GGTNACC 1 cut(s) 6500
EcoRI GAATTC 2 cut(s) 2535, 6159
EcoRV GATATC 4 cut(s) 3585, 4067, 4684, 6230
EcoT22I ATGCAT 2 cut(s) 1090, 2455
EcoT38I GRGCYC 2 cut(s) 5183, 6478
FalI AAGNNNNNCTT 8 cut(s) 454, 486, 3196, 3228, 6781, 6813, 7341, 7373
FaqI GGGAC 7 cut(s) 432, 1449, 2272, 4345, 4822, 5371, 6745
FauI CCCGC 1 cut(s) 4847
FauNDI CATATG 7 cut(s) 221, 403, 985, 1066, 1223, 4832, 7301
FbaI TGATCA 2 cut(s) 3538, 4051
FblI GTMKAC 3 cut(s) 321, 5157, 5886
FriOI GRGCYC 2 cut(s) 5183, 6478
FspI TGCGCA 2 cut(s) 1442, 3833
GlaI GCGC 5 cut(s) 1056, 1442, 2126, 3768, 3833
GsaI CCCAGC 2 cut(s) 2199, 4332
GsuI CTGGAG 5 cut(s) 85, 839, 5487, 5655, 6350
HaeII RGCGCY 1 cut(s) 2128
HapII CCGG 4 cut(s) 958, 995, 5450, 6081
HgaI GACGC 1 cut(s) 3309
HhaI GCGC 5 cut(s) 1057, 1443, 2127, 3769, 3834
Hin1I GRCGYC 1 cut(s) 5223
Hin6I GCGC 5 cut(s) 1055, 1441, 2125, 3767, 3832
HinP1I GCGC 5 cut(s) 1055, 1441, 2125, 3767, 3832
HincII GTYRAC 4 cut(s) 942, 4642, 5227, 6576
HindII GTYRAC 4 cut(s) 942, 4642, 5227, 6576
HindIII AAGCTT 6 cut(s) 766, 2259, 2526, 3205, 5344, 6346
HpaI GTTAAC 1 cut(s) 4642
HpaII CCGG 4 cut(s) 958, 995, 5450, 6081
HphI GGTGA 9 cut(s) 300, 3870, 4274, 4610, 5158, 6983, 7346, 7380, 7384
HpyCH4IV ACGT 8 cut(s) 1077, 3286, 3302, 3569, 3912, 4503, 5223, 5883
HpySE526I ACGT 8 cut(s) 1077, 3286, 3302, 3569, 3912, 4503, 5223, 5883
Hsp92I GRCGYC 1 cut(s) 5223
HspAI GCGC 5 cut(s) 1055, 1441, 2125, 3767, 3832
KflI GGGWCCC 1 cut(s) 4332
Kpn2I TCCGGA 1 cut(s) 957
KpnI GGTACC 1 cut(s) 21
Ksp22I TGATCA 2 cut(s) 3538, 4051
KspAI GTTAAC 1 cut(s) 4642
MabI ACCWGGT 2 cut(s) 1495, 2680
MaeII ACGT 8 cut(s) 1077, 3286, 3302, 3569, 3912, 4503, 5223, 5883
MfeI CAATTG 1 cut(s) 1425
MflI RGATCY 8 cut(s) 911, 2427, 3075, 3691, 4818, 5518, 6868, 7353
MhlI GDGCHC 8 cut(s) 4695, 5183, 5426, 6247, 6277, 6478, 6593, 7241
MlsI TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
MluNI TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
MmeI TCCRAC 3 cut(s) 487, 2748, 3126
Mox20I TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
Mph1103I ATGCAT 2 cut(s) 1090, 2455
MroI TCCGGA 1 cut(s) 957
MroXI GAANNNNTTC 6 cut(s) 2923, 3155, 4303, 4350, 5246, 7272
MscI TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
Msp20I TGGCCA 6 cut(s) 2932, 3298, 5431, 5837, 7115, 7298
MspA1I CMGCKG 3 cut(s) 50, 2582, 5678
MspI CCGG 4 cut(s) 958, 995, 5450, 6081
MssI GTTTAAAC 1 cut(s) 2677
MunI CAATTG 1 cut(s) 1425
Mva1269I GAATGC 7 cut(s) 57, 886, 1088, 2956, 3226, 3489, 5248
NcoI CCATGG 4 cut(s) 1768, 5251, 7043, 7110
NdeI CATATG 7 cut(s) 221, 403, 985, 1066, 1223, 4832, 7301
NheI GCTAGC 1 cut(s) 5540
NmeAIII GCCGAG 2 cut(s) 1432, 7134
NmuCI GTSAC 4 cut(s) 4262, 4826, 5870, 7006
NsbI TGCGCA 2 cut(s) 1442, 3833
NsiI ATGCAT 2 cut(s) 1090, 2455
NspI RCATGY 9 cut(s) 2739, 3448, 3643, 3925, 4846, 5555, 5714, 6344, 7399
NspV TTCGAA 1 cut(s) 1776
OliI CACNNNNGTG 1 cut(s) 259
PaeI GCATGC 3 cut(s) 2739, 4846, 5714
PceI AGGCCT 1 cut(s) 4284
PciI ACATGT 1 cut(s) 3921
PcsI WCGNNNNNNNCGW 2 cut(s) 1796, 6238
PctI GAATGC 7 cut(s) 57, 886, 1088, 2956, 3226, 3489, 5248
PdmI GAANNNNTTC 6 cut(s) 2923, 3155, 4303, 4350, 5246, 7272
PflMI CCANNNNNTGG 3 cut(s) 2767, 3688, 5438
PfoI TCCNGGA 1 cut(s) 2626
PmeI GTTTAAAC 1 cut(s) 2677
Ppu21I YACGTR 1 cut(s) 3287
PpuMI RGGWCCY 4 cut(s) 4332, 5299, 6898, 7106
PscI ACATGT 1 cut(s) 3921
PshBI ATTAAT 1 cut(s) 6216
PsiI TTATAA 5 cut(s) 3491, 3840, 3945, 5858, 7025
Psp124BI GAGCTC 1 cut(s) 5183
Psp1406I AACGTT 1 cut(s) 3302
Psp5II RGGWCCY 4 cut(s) 4332, 5299, 6898, 7106
PspEI GGTNACC 1 cut(s) 6500
PspFI CCCAGC 2 cut(s) 2195, 4328
PspPPI RGGWCCY 4 cut(s) 4332, 5299, 6898, 7106
PsrI GAACNNNNNNTAC 4 cut(s) 1508, 1540, 3902, 3934
PstI CTGCAG 4 cut(s) 4776, 4809, 5307, 7015
PstNI CAGNNNCTG 3 cut(s) 3441, 5678, 7182
PsuI RGATCY 8 cut(s) 911, 2427, 3075, 3691, 4818, 5518, 6868, 7353
PvuII CAGCTG 3 cut(s) 50, 2582, 5678
SacI GAGCTC 1 cut(s) 5183
ScaI AGTACT 4 cut(s) 2785, 3341, 3827, 4303
SduI GDGCHC 8 cut(s) 4695, 5183, 5426, 6247, 6277, 6478, 6593, 7241
SexAI ACCWGGT 2 cut(s) 1495, 2680
SfcI CTRYAG 8 cut(s) 2176, 4772, 4805, 5217, 5233, 5303, 5350, 7011
SfuI TTCGAA 1 cut(s) 1776
SmlI CTYRAG 4 cut(s) 2182, 3173, 3335, 6648
SmoI CTYRAG 4 cut(s) 2182, 3173, 3335, 6648
SnaBI TACGTA 1 cut(s) 3287
SphI GCATGC 3 cut(s) 2739, 4846, 5714
SseBI AGGCCT 1 cut(s) 4284
SsiI CCGC 6 cut(s) 2401, 4260, 4840, 4846, 7092, 7219
SspI AATATT 5 cut(s) 1296, 2647, 3017, 4666, 6845
SstI GAGCTC 1 cut(s) 5183
StuI AGGCCT 1 cut(s) 4284
TaiI ACGT 8 cut(s) 1080, 3289, 3305, 3572, 3915, 4506, 5226, 5886
TaqI TCGA 8 cut(s) 231, 1114, 1386, 1776, 4063, 6232, 6609, 6765
TaqII GACCGA 3 cut(s) 767, 2059, 5976
TauI GCSGC 1 cut(s) 7221
TseFI GTSAC 4 cut(s) 4262, 4826, 5870, 7006
Tsp45I GTSAC 4 cut(s) 4262, 4826, 5870, 7006
TspGWI ACGGA 6 cut(s) 1094, 1855, 2529, 2657, 4814, 6994
Van91I CCANNNNNTGG 3 cut(s) 2767, 3688, 5438
VneI GTGCAC 1 cut(s) 6273
VspI ATTAAT 1 cut(s) 6216
XagI CCTNNNNNAGG 4 cut(s) 1592, 1703, 4416, 7285
XapI RAATTY 7 cut(s) 2234, 2535, 3137, 4456, 6159, 6204, 6999
XbaI TCTAGA 3 cut(s) 908, 2695, 7402
XceI RCATGY 9 cut(s) 2739, 3448, 3643, 3925, 4846, 5555, 5714, 6344, 7399
XmiI GTMKAC 3 cut(s) 321, 5157, 5886
XmnI GAANNNNTTC 6 cut(s) 2923, 3155, 4303, 4350, 5246, 7272
ZraI GACGTC 1 cut(s) 5224
ZrmI AGTACT 4 cut(s) 2785, 3341, 3827, 4303
Zsp2I ATGCAT 2 cut(s) 1090, 2455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.