MD03G1271800.v1.1

Piezo non-specific cation channel, R-Ras-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006607
Physical Location & Seq
Forward (+)
35494632 .. 35509342
14711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1271800.v1.1.491

Sequence Viewer

Length: 7467 bp
ATGGGGAGATTTCTTGGCGGGTTTGTGTTGCCTCTGCTGCTGCTAGCAGCTGCTATTATCAACTGGAGCGTCCTCTCTCTGGTCAATTTGATAGCGTTTCTTCTTATTCTATTTAATGCACCGAAACTAGGATTTCATTTTGGAAGGCGGCTTTTATTGACATGGATCATTGTTATATTTTCTTTATTTGTTATTTTTTCTCAAGTGATATATCTTGTTATATGGGCTATTGAGGGAAATAAATGGAGCGGAGTGGATGCTTGGTGGGCAAATCTTATTGGATTCATGATATTGCAGTCATGGAAATCTCCTCTCGTGCTATACTTTTTACTCTTACAACTATCAGTAGTTGCTGTTGCTTTAGCTGATTTGTATGGAAACAGATTTGGTCTTGTTTCATCATGTGATTCATGTTGGGGTCATTTCTCATCAGCCATTGAACGACTAATTTGTTCTCATCTTAGAGTTGCTGCCTTGTTGCTGTTGCCTGCCATTCAGTTGGTTGTTGGAATTAGCCGTCCTTCATGGGTTTCTCTACCCTTTTTTATTGGGAGCTGCATTGGTCTTGTGGATTGGTCTTTGTCAAGTAATTTTCTAGGACTTTTCAGGTGGTGGAAGCCTTTTCACTTGTATGCAGGTTTCAACATTGTCTTGCTTTATGCGTATCAGCTCCCTGTGGAGTTTCCAGATATGTTACGATGGGTAGCTGAGTTCATTGGTCTATTCAAAATAACTTTACATTCGGACTGGACTGAATTTTGTTCATGCTGCTCTCTTTTACTTTTCTACATCATGCTATCCTGCGTTAAATGTGATCTAGAGGAAATGGATTTTATTTTATCCATGAAAGAAAATAACTTGACGGAGCAACTTCTTCCCTCGAAGCATTCATTTTTTATTCGTGAATCTAGATCTGGTGTAAGGCATACCAATGTTTTATTGACTGGAGCGGTTTTCCGGACCTTTAGTATCAACTTCTTCACATATGGTTTCCCAGTTTCCTTGTTTGCTCTTTCATTTTGGAGTTTTCATTTTGCAAGTATATGTGCATTTTTGCTACTTGCATATGTTGGCTACATCATATATGCCTTCCCTTCCTTATTCCGTTTGCATCGACTGAATGGGCTGCTGCTTGTCTTCATTCTCTTGTGGGCTGCTAGCACGTATATTTTCAATGTAGCATTTGCATTCTTGAATCGGAAAATCGGGAAGAACATGGACATATGGGAGATGATTGGGTTGTGGCATTATCCCATACCTGGATTCTTTCTGCTTGCACAATTTTGTCTTGGAATACTGGTTGCCTTGGGTAATCTTGTGAACAACTCGGTTTTCCTTTGCTTGTCTGATGAGGATGGACAACCTTCAAATGACAACAGTACTGTAGAAGGCGAGGGAGAGACCAAAGTATTGATAGTGGCCACAATTGCTTGGGGACTGCGCAAAAGTTCCCGAGCTATCATGCTGGCACTGATATTTCTAATTGCCATGAAACCTGGTTTCATCCATGCTGTATATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACATTAACAGAAAGATGCGCCAGTCTTTGATTCTCCTATGTGAGGTTCACTTTGCACTATTGTATATCATTCAGATTAATCCGATCTCTGATACTTTGGAGAAAAAAGGCACTTTGAGTGCCGAAATCTTATCACAATTAGGTCTTCTTCAACATGAAAGCTCGTGGGACTTTTTGCAAATAGCTTTGCTTGCTTGCTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTATTTTCGTTCTCAGCAATTGTGCAGCATACACCTAGCCGTCCATTTGGTTTCAGCATTTTGAAAGCTGGTCTGAATAAATCAGTTTTGCTGTCAGTGTATGCCTCCTCAGCCATTCAGTACAGCCATGATAATCCTTCTTATGAGAGAAGAATTGCATTATTCCTCGGTGCAATTGGGCAGAAGTTTTTATCTGTGTACCGATCATGTGGAACCTACATTGCCTTTCTGACAATTCTCCTTACTGTATACTTGGTGAGACCCAACTATATATCATTTGGGTACATATTCCTTCTCCTTGTTTGGATCATTGGAAGACAACTTGTCGAGAGAACAAAAAAGCGCCTTTGGTTCCCATTGAAAGCATATGCAATTGTGGTGTTTATCTTTATCTATAGCTTGAGCAGTTTCCCGAGCATTGAGCTGTGGTTGTCCAAGTTCATAGACCTTTACTTTTATCTAGGTTATGACTCAGAAGCTTCATCTTTGCAAAATGTTTGGGAATCCCTAGCAGTCTTGATTGTGATGCAACTTTATAGCTATGAGAGAAGGCAGAGCAGGTATAACAGGTCAGATGATGCCCATGTGTTGGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTGTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTATCTCCAATAAGTGCCTTTGGCTTCTTTTATCTACTTGGCCTCGTCATCTGTTCATCTTTACCTAAAGCTTCACATTTCCCATCTAAATCATTCTTGGTTTACACAGGATTTCTAGTAACAACTGAGTACCTTTTTCAGATGTGGGGTAGACAGGCTGCAATGTTTCCTGGACAAAAGCACTCTTATATTTCCCTTTTTTTGGGTTTCCGTGTATTTAAACCGGGATTTTGGGGTCTAGAATCTGGCTTGAGAGGAAAAGTTCTGGTGATTGCTGCATGCACTCTTCAATATAATGTTTTTCGCTGGTTGGAAAAGATGCCAAGTACTATCCTAAACAAAGGAAAGTGGGAAGAGCCTTGTCCATTGTTTGTCTCAGCAGAAGATGCCGATGCCAATATTAATGGCTCCATCCCAAGTGAGGATAACAAGCAATCAACAGATTCCGAAGCGATTTCAGTGAAACGAGAAGGGGCTAGGAGCCAATCATGGCCCTTTTTTATCCCTAGTTCATCGCAGTTGCCTAACCATGTGTCCCCTAAAGCAGGAGATTCTGAGGGTAGTAGCAGTAACAAATATTCATTTGGGTACATTTGGGGCAGCACCAAGGAGAGTCATAAGTGGAACAAGAAGCGGATTCTTGCCTTGAGAAAGGAGAGATTTGACATACAGAAGCTTATTGCAAAAATCTATTTGAAATTCTGGATGGAGAATATGTTTAACCTCTTTGGTCTTGAGATAAACATGATTGCATTGCTTCTGGCAAGCTTTGCTTTATTGAATGCCATTTCTATGGTATACATTGCGTTACTTGCTACATGTATTATTTTGAATCGGCATATTATACGTAAACTATGGCCCATATTAGTTTTCCTGTTTGCATCCATTCTCATCCTCGAGTACTTTGCCATCTGGAGGAGTCTGTGGTCTCTAAGTCAGCCTGATGAGACTAATGCACATTGCCATGATTGCTGGAAAAGCTCAGCCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTATTGTCGATGATCCGCGAATGCTTATCAGCTACTTTGCTGTCTTCATGCTTGCTTGTTTTAAACTTCGTGCTGATAATTTGTCCGGATTCTCGGTGTCATCAACTTACCGTCAAGTGATATCTCAACGTAATAATTTATTTGTGTGGAGAGACCTCTCTTTCGAAACCAAAAGCATGTGGACCTTCTTTGACTACCTGAGGCTTTACTGCTACTGCCATCTATTGGATCTTGTGCTAGCATTGGTGTTGATTACTGGAACTATTGAGTATGACATTCTGCACCTTGGTTATCTTGCTTTTGCGCTGGTTTTCTTTCGGGGGAGACTTGAAATACTAAAGAAGAGGAACAAGATATTTAAGTTCTTGCGCATATACAACTTTGCTCTTATCGTTCTTTCTCTTGCATACCAATCTCCTTTCGTGGGGGAGTTCTGTGCTGGAAAGTGTGAGACCGTAGATTACGTATTTGAGATGATTGGATTTTATAAGTATGATTATGGGTTTCGGATTACTGCAAGATCAGCTCTCGTTGAAATTATCATTTTTATGCTGGTATCACTTCAGTCCTATATGTTCTCCTCCCCAGAGTTTGATTATGTTTCTCGATATCTTGAAGCCGAGCAAATTGGTGCAATTGTGCGTGAGCAAGAAAAGAAAGCTGCATGGAAAACTGCACAGTTAAAACATATTCGTGAATCTGAGGAGAAGAAACACCAGCGTAACCTGCAAGTGGAGAAAATGAAGTCAGAGATGCTCAACCTGCAAATCCAGCTCCACAGCATGAATTCAGTTACTAATTGCGGTGACTCTCCTGCAGTCAGTGAAGGCCTAAGAAGGAGGAGGAGTACTTCTCTTAACTCGAACAACGATCAGGGGACCTCTGACAAAGAAGGATTACAACTGAAAAAGGAACAGATACTTAGAGAGGATTCATTATATCCCTTTGAATTGCATGAATCTCCTGCACCAGTGAATGTGGAAACCCCAACAGTGATGGAGTCTGCGAGAGATTCAATAGAATCTCTTAACTGTGAGATCACTGAAGTTGACGATGATGTTGCTGATGGCATATTTTTCACTTCTTCAGAAAAGAAGGACAAAGTTAAAGGGAAAGCAAAGGAAAGCCCACTAATATCTGCAGTTCATCTGCTAGGTGATGGTGTTTCCCAGGTACAGTCGATTGGAAATCAGGCAGTTAACAACCTTGTAAGCTTTTTGAACATTGATCATGAGTTTGACGTCAGTGAGCACTCTTCTGTTGAGGATGGGGTATATGATGAAATGGAGAGCCAGAAAGTTAAGGTGTCCTTTAACCGTTCATCTTCCGTTCGGTCCGACATGAGTTCTGATGCAACAAGTCTGCAGTTAGGAAGGATCCTCCGTCACATATGGTCCCAAATGCGATCTAACAATGATATTGTGTGTTATTGTTGTTTTATCCTTGTCTTTTTATGGAACTTCAGTTTGCTTTCTATGGTGTATCTGGCGGCTTTATTCTTGTACGCACTATGTGTAAATTCTGGTCCAAGTTACATCTTCTGGGTTGTTATGCTGATTTACACTGAAGTCTATATTTTGCTTCAGTATCTGTACCAGATTATCATCCAGCACTGGGCTTTCAGTGTTGCTTCAGACTTGCTTCGCGAGTGGGGATTTCCTGAACATAAAATCACATCTTCTTTTGTGGTCAGTTCATTACCTCTCTTTCTTGTCTACTTATTTACCCTCTTACAGAGCTCTATAACCGCAAAAGATGGTGAGTGGATGTCGTCTACAGACTTCGACTTCTATAGGAGGAGTGCTTTCCATGGGAAAGAGATTCCTGTCAGTTATAGCTGGAGTCAGAAAATAAAGAAGTTGCTGCAAATAATGGAAAATGCAATAAAATCAATAATCAGAAGCTTCTTTAGGTACTGGAATTCACTGACACAGGGAGCAGATTCCCCTCCTTACTTTATTCAGGTGTCTATGGATGTGCGCTCATGGCCAGAGGACGGGATTCAGCCAGAAAAGATTGAGTCTGGAGTAAATCAATTGCTTAAAATCATTCATGATGAAAGATGCAAGGAGAAAACCCCTAATCTATGCCCTTTTGCTAGCAGGGTGCAGGTCCAAAGCATTGAAAGGAGTCAAGAAAATGCAAATGTAGCCTTGGTTGTTTTTGAGGTGGTATATGCCTCCCCCGTGACAGAGTGTGGCAATTCAATAGAATGGTACAAGTCACTTACTCCAGCAGCTGATGTGGCGAAGGAAATTCTTAAAGCACAAGATGCTGGGTTTGTTGAAGAAATAGGATTTCCATACCCTATACTCTCTGTTATTGGAGGAGGCAAGAGGGACATTGATCTGTATGCATATGTATTTGGTGCCGATCTGACTGTTTTCTTTTTAGTCGCCATGTTCTACCAATCCGTCATAAAAAATAAAAGTGAGTTTCTTGATGTGTATCAGCTTGAAGATCAGTTTCCGAAAGAGTTTGTATTCATCTTAATGATTATCTTTTTCTTGATTGTGCTTGACCGCATAATCTATCTCTGTTCATTTGCCACCGGAAAAGTGATTTTCTATCTTTTCAACCTCATCCTCTTCACATATTCAGTAACAGAGTATGCTTGGAACATGGAGCCTTTCCACCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAAGCAGTTTCTTTAGCACTGCAGGCTATACAACTCCGACATGGAATTCCTCATAAAAGCACTTTGTACCGGCAGTTTTTGACTAGTGAGATTTCAAGAATTAATTATTTGGGATATCGACTATATCGCGCTCTGCCTTTCCTTTATGAACTGAGATGTGCACTTGACTGGTCATGCACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTTGTCAAATGTGATGCAGTCTTGAATAGAGCAACGCACAAACAAGGAGATAAGCAAACGAAAATGACCAAATGTTGCAACGGGATATGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTACAGCAGTGGTAACCCGACGAACATTGAAAATCCCATCAAAGATGCCAGTGTTCAGTTTGACATAAAGACAGCGAGTGGAAGATTGACCCTATATCAAACCACCCTTTGTGAAAAGTTCCAATGGGACAAACTCGACTCTGATGTTAATCTCGATCCTGAAGGTTATCTGGATACCTATAATAAGAAAGACGTCCAGTTGATATGTTGCGAATCTGATGCTAGTACTTTGTGGCTTATCCCCGATGTAGTTCAAACCAGATTCATTCGGTCCCTTGATTGGGACCCCAACATGGCTATATCTTTTACTTGGGTACTTTCCAGGGACAGACCCAAGGGCAAGGAAACTGTGAAATATGAAAGAAGTTTGGAGTCTCAGGATCTTCCAAAACAGTCAGATGTCCAAAAGGTTCTCAATGGCTCCCAAAACAGCTTTAGGATATACAACATTTATCCAAGATACTTCCGTGTCACTGGTTCTGGTGATGTCAGACTGTTGGAATTAGAGGATAAGTTCGTTAGCGCAGACCTTGTTCTGAATCGCTCAAATTATGAGTGGTGGTCCTTCCACGACATCAATTCATCAGATGTCAATGGATGTGGAGGTTTGACAGGACCGATGGCCATCATCGTATCTGAGGAAACACCGCCACAGGGTATTCTCGGTGACACCCTGAGCAAGTTCAGCATTTGGGGTCTATACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAATGCTCTGACTTACGAATGAGAATCCCCTATGAGAACCTTCCTTCCTGTGATAGGTTGATAGCCATCTGTGAAGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGAGTTGAAGAGGTCTTATACTGGACGCTGGTGAAGATTTACAGGTCACCACACATGCTGCTCGAATACACGAAACCGGATTAG

Protein Analysis

2489

Amino Acids

285.34

Weight (kDa)

7.44

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIEZO PF25288 472 - 601 1.5e-64 Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1 PF23188 1630 - 1749 1.1e-17 Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor PF24874 1943 - 2180 8.1e-65 Piezo, THU9 and anchor domain
Piezo_cap PF12166 2208 - 2487 1.2e-80 Piezo non-specific cation channel, cap domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3969
AatII GACGTC 2 cut(s) 4725, 6729
Acc16I TGCGCA 2 cut(s) 1442, 3851
Acc36I ACCTGC 5 cut(s) 626, 2331, 4215, 4251, 5581
AccB1I GGYRCC 1 cut(s) 5849
AccB7I CCANNNNNTGG 2 cut(s) 2371, 3706
AccBSI CCGCTC 2 cut(s) 249, 950
AccI GTMKAC 5 cut(s) 2031, 2608, 3255, 5196, 5255
AccII CGCG 3 cut(s) 3499, 5127, 6288
AccIII TCCGGA 2 cut(s) 957, 3566
AclWI GGATC 8 cut(s) 173, 2096, 3488, 3717, 4852, 4865, 6683, 6921
AcoI YGGCCR 4 cut(s) 1419, 5468, 7155, 7258
AcsI RAATTY 8 cut(s) 755, 2375, 3155, 4267, 4999, 5401, 5736, 6204
AcuI CTGAAG 8 cut(s) 4028, 4545, 4551, 4927, 5048, 5067, 5097, 6714
AcyI GRCGYC 2 cut(s) 4722, 6726
AdeI CACNNNGTG 2 cut(s) 4994, 6143
AflIII ACRYGT 1 cut(s) 3275
AhdI GACNNNNNGTC 2 cut(s) 2105, 3485
AhlI ACTAGT 1 cut(s) 6242
AjnI CCWGG 5 cut(s) 1258, 1495, 2626, 4650, 6854
AloI GAACNNNNNNTCC 2 cut(s) 7304, 7336
Alw21I GWGCWC 3 cut(s) 4734, 5222, 6322
Alw26I GTCTC 9 cut(s) 1394, 2035, 2836, 3390, 3398, 3627, 3799, 3926, 6912
Alw44I GTGCAC 1 cut(s) 6318
AlwI GGATC 8 cut(s) 173, 2096, 3488, 3717, 4852, 4865, 6683, 6921
AlwNI CAGNNNCTG 3 cut(s) 3459, 5071, 5720
Ama87I CYCGRG 3 cut(s) 1452, 2194, 3353
Aor13HI TCCGGA 2 cut(s) 957, 3566
AoxI GGCC 8 cut(s) 1419, 2497, 2948, 3314, 4309, 5468, 7155, 7258
ApaLI GTGCAC 1 cut(s) 6318
ApoI RAATTY 8 cut(s) 755, 2375, 3155, 4267, 4999, 5401, 5736, 6204
ArsI GACNNNNNNTTYG 2 cut(s) 3626, 3658
AseI ATTAAT 3 cut(s) 1629, 2859, 6261
AspLEI GCGC 8 cut(s) 1443, 1572, 2127, 3787, 3852, 5463, 6290, 7058
AsuC2I CCSGG 1 cut(s) 2682
AsuII TTCGAA 1 cut(s) 3645
AsuNHI GCTAGC 4 cut(s) 43, 1157, 3718, 5579
AvaI CYCGRG 3 cut(s) 1452, 2194, 3353
AxyI CCTNAGG 1 cut(s) 3680
BaeGI GKGCMC 1 cut(s) 6322
BaeI ACNNNNGTAYC 2 cut(s) 6985, 7018
BalI TGGCCA 3 cut(s) 1421, 5470, 7157
BamHI GGATCC 1 cut(s) 4857
BanI GGYRCC 1 cut(s) 5849
BanII GRGCYC 2 cut(s) 5222, 6523
BauI CACGAG 2 cut(s) 314, 1714
BbsI GAAGAC 4 cut(s) 1129, 1688, 2104, 3517
Bbv12I GWGCWC 3 cut(s) 4734, 5222, 6322
BbvCI CCTCAGC 1 cut(s) 1891
BceAI ACGGC 2 cut(s) 501, 1806
BcgI CGANNNNNNTGC 6 cut(s) 3344, 3378, 4523, 4557, 6734, 6768
BciT130I CCWGG 5 cut(s) 1260, 1497, 2628, 4652, 6856
BciVI GTATCC 1 cut(s) 6700
BclI TGATCA 1 cut(s) 4708
BcnI CCSGG 1 cut(s) 2682
BcoDI GTCTC 9 cut(s) 1394, 2035, 2836, 3390, 3398, 3627, 3799, 3926, 6912
BcuI ACTAGT 1 cut(s) 6242
BfmI CTRYAG 8 cut(s) 1383, 2176, 4296, 4620, 4844, 5256, 5272, 6179
BfoI RGCGCY 1 cut(s) 2128
BfuAI ACCTGC 5 cut(s) 626, 2331, 4215, 4251, 5581
BfuI GTATCC 1 cut(s) 6700
BglII AGATCT 2 cut(s) 911, 2427
BlpI GCTNAGC 1 cut(s) 3439
BmcAI AGTACT 6 cut(s) 1381, 2785, 3359, 3467, 4330, 6760
Bme1390I CCNGG 6 cut(s) 1260, 1497, 2628, 2682, 4652, 6856
BmeRI GACNNNNNGTC 2 cut(s) 2105, 3485
BmeT110I CYCGRG 3 cut(s) 1452, 2194, 3353
BmrFI CCNGG 6 cut(s) 1260, 1497, 2628, 2682, 4652, 6856
BmrI ACTGGG 2 cut(s) 989, 5104
BmtI GCTAGC 4 cut(s) 47, 1161, 3722, 5583
BmuI ACTGGG 2 cut(s) 989, 5104
BpiI GAAGAC 4 cut(s) 1129, 1688, 2104, 3517
BplI GAGNNNNNCTC 2 cut(s) 4318, 4350
BpmI CTGGAG 8 cut(s) 85, 966, 3391, 5341, 5526, 5697, 6147, 6395
Bpu10I CCTNAGC 2 cut(s) 1891, 7208
Bpu1102I GCTNAGC 1 cut(s) 3439
Bpu14I TTCGAA 1 cut(s) 3645
BpuEI CTTGAG 5 cut(s) 186, 2203, 2728, 3124, 3212
BpuMI CCSGG 1 cut(s) 2682
BsaAI YACGTR 3 cut(s) 1164, 3305, 3946
BsaBI GATNNNNATC 5 cut(s) 5084, 5928, 6681, 7157, 7340
BsaHI GRCGYC 2 cut(s) 4722, 6726
BsaI GGTCTC 5 cut(s) 1394, 2035, 3390, 3627, 3926
BsaWI WCCGGW 4 cut(s) 957, 3566, 6032, 7459
BsaXI ACNNNNNCTCC 4 cut(s) 3158, 3188, 4312, 4342
Bse118I RCCGGY 1 cut(s) 6228
Bse21I CCTNAGG 1 cut(s) 3680
Bse3DI GCAATG 5 cut(s) 2001, 2625, 3209, 3258, 3415
Bse8I GATNNNNATC 5 cut(s) 5084, 5928, 6681, 7157, 7340
BseAI TCCGGA 2 cut(s) 957, 3566
BseBI CCWGG 5 cut(s) 1260, 1497, 2628, 4652, 6856
BseJI GATNNNNATC 5 cut(s) 5084, 5928, 6681, 7157, 7340
BseMI GCAATG 5 cut(s) 2001, 2625, 3209, 3258, 3415
BseRI GAGGAG 9 cut(s) 300, 1879, 3388, 4051, 4199, 4336, 4339, 5293, 5823
BseSI GKGCMC 1 cut(s) 6322
BseYI CCCAGC 1 cut(s) 5756
BsgI GTGCAG 5 cut(s) 1826, 3746, 4140, 4431, 5609
Bsh1236I CGCG 3 cut(s) 3499, 5127, 6288
BshFI GGCC 8 cut(s) 1421, 2499, 2950, 3316, 4311, 5470, 7157, 7260
BshNI GGYRCC 1 cut(s) 5849
BsiHKAI GWGCWC 3 cut(s) 4734, 5222, 6322
BsiHKCI CYCGRG 3 cut(s) 1452, 2194, 3353
BsiSI CCGG 6 cut(s) 958, 2681, 3567, 6033, 6229, 7460
BsmAI GTCTC 9 cut(s) 1394, 2035, 2836, 3390, 3398, 3627, 3799, 3926, 6912
BsmI GAATGC 4 cut(s) 886, 1187, 3244, 3507
BsnI GGCC 8 cut(s) 1421, 2499, 2950, 3316, 4311, 5470, 7157, 7260
Bso31I GGTCTC 5 cut(s) 1394, 2035, 3390, 3627, 3926
BsoBI CYCGRG 3 cut(s) 1452, 2194, 3353
Bsp119I TTCGAA 1 cut(s) 3645
Bsp1286I GDGCHC 4 cut(s) 4734, 5222, 6322, 6523
Bsp13I TCCGGA 2 cut(s) 957, 3566
Bsp1407I TGTACA 1 cut(s) 6534
Bsp1720I GCTNAGC 1 cut(s) 3439
Bsp19I CCATGG 2 cut(s) 1768, 5290
Bsp68I TCGCGA 1 cut(s) 5127
BspANI GGCC 8 cut(s) 1421, 2499, 2950, 3316, 4311, 5470, 7157, 7260
BspEI TCCGGA 2 cut(s) 957, 3566
BspFNI CGCG 3 cut(s) 3499, 5127, 6288
BspHI TCATGA 3 cut(s) 285, 4711, 5533
BspMAI CTGCAG 4 cut(s) 4300, 4624, 4848, 6183
BspMI ACCTGC 5 cut(s) 626, 2331, 4215, 4251, 5581
BspOI GCTAGC 4 cut(s) 47, 1161, 3722, 5583
BspPI GGATC 8 cut(s) 173, 2096, 3488, 3717, 4852, 4865, 6683, 6921
BspQI GCTCTTC 1 cut(s) 2805
BspT104I TTCGAA 1 cut(s) 3645
BspT107I GGYRCC 1 cut(s) 5849
BspTNI GGTCTC 5 cut(s) 1394, 2035, 3390, 3627, 3926
BsrBI CCGCTC 2 cut(s) 249, 950
BsrDI GCAATG 5 cut(s) 2001, 2625, 3209, 3258, 3415
BsrFI RCCGGY 1 cut(s) 6228
BsrGI TGTACA 1 cut(s) 6534
BssAI RCCGGY 1 cut(s) 6228
BssNAI GTATAC 2 cut(s) 2032, 3256
BssNI GRCGYC 2 cut(s) 4722, 6726
BssSI CACGAG 2 cut(s) 314, 1714
BssT1I CCWWGG 7 cut(s) 1305, 1768, 3063, 3766, 5290, 5634, 6867
Bst1107I GTATAC 2 cut(s) 2032, 3256
Bst2BI CACGAG 2 cut(s) 314, 1714
Bst2UI CCWGG 5 cut(s) 1260, 1497, 2628, 4652, 6856
Bst6I CTCTTC 6 cut(s) 2748, 2805, 3818, 4741, 6074, 7386
BstACI GRCGYC 2 cut(s) 4722, 6726
BstAPI GCANNNNNTGC 5 cut(s) 2843, 3227, 3468, 5753, 6143
BstAUI TGTACA 1 cut(s) 6534
BstBAI YACGTR 3 cut(s) 1164, 3305, 3946
BstBI TTCGAA 1 cut(s) 3645
BstDSI CCRYGG 2 cut(s) 1768, 5290
BstEII GGTNACC 2 cut(s) 6545, 7428
BstENI CCTNNNNNAGG 3 cut(s) 1592, 3000, 7327
BstFNI CGCG 3 cut(s) 3499, 5127, 6288
BstH2I RGCGCY 1 cut(s) 2128
BstHHI GCGC 8 cut(s) 1443, 1572, 2127, 3787, 3852, 5463, 6290, 7058
BstMAI GTCTC 9 cut(s) 1394, 2035, 2836, 3390, 3398, 3627, 3799, 3926, 6912
BstNI CCWGG 5 cut(s) 1260, 1497, 2628, 4652, 6856
BstNSI RCATGY 6 cut(s) 2739, 3279, 3661, 6125, 6389, 7441
BstPI GGTNACC 2 cut(s) 6545, 7428
BstSCI CCNGG 6 cut(s) 1258, 1495, 2626, 2680, 4650, 6854
BstSFI CTRYAG 8 cut(s) 1383, 2176, 4296, 4620, 4844, 5256, 5272, 6179
BstSLI GKGCMC 1 cut(s) 6322
BstSNI TACGTA 2 cut(s) 3305, 3946
BstUI CGCG 3 cut(s) 3499, 5127, 6288
BstV2I GAAGAC 4 cut(s) 1129, 1688, 2104, 3517
BstX2I RGATCY 5 cut(s) 911, 2427, 3709, 4857, 6913
BstXI CCANNNNNNTGG 3 cut(s) 499, 3250, 6125
BstYI RGATCY 5 cut(s) 911, 2427, 3709, 4857, 6913
BstZ17I GTATAC 2 cut(s) 2032, 3256
Bsu36I CCTNAGG 1 cut(s) 3680
BsuI GTATCC 1 cut(s) 6700
BsuRI GGCC 8 cut(s) 1421, 2499, 2950, 3316, 4311, 5470, 7157, 7260
BtgI CCRYGG 2 cut(s) 1768, 5290
BtgZI GCGATG 1 cut(s) 2955
BtsI GCAGTG 2 cut(s) 6176, 6547
BtuMI TCGCGA 1 cut(s) 5127
BveI ACCTGC 5 cut(s) 626, 2331, 4215, 4251, 5581
CaiI CAGNNNCTG 3 cut(s) 3459, 5071, 5720
CciI TCATGA 3 cut(s) 285, 4711, 5533
CfoI GCGC 8 cut(s) 1443, 1572, 2127, 3787, 3852, 5463, 6290, 7058
Cfr10I RCCGGY 1 cut(s) 6228
CpoI CGGWCCG 1 cut(s) 4815
CseI GACGC 2 cut(s) 58, 7417
CsiI ACCWGGT 1 cut(s) 1495
CspCI CAANNNNNGTGG 2 cut(s) 7114, 7149
CspI CGGWCCG 1 cut(s) 4815
DraI TTTAAA 2 cut(s) 2677, 3544
DraIII CACNNNGTG 2 cut(s) 4994, 6143
DriI GACNNNNNGTC 2 cut(s) 2105, 3485
EaeI YGGCCR 4 cut(s) 1419, 5468, 7155, 7258
Eam1104I CTCTTC 6 cut(s) 2748, 2805, 3818, 4741, 6074, 7386
Eam1105I GACNNNNNGTC 2 cut(s) 2105, 3485
EarI CTCTTC 6 cut(s) 2748, 2805, 3818, 4741, 6074, 7386
Ecl136II GAGCTC 1 cut(s) 5220
Eco105I TACGTA 2 cut(s) 3305, 3946
Eco130I CCWWGG 7 cut(s) 1305, 1768, 3063, 3766, 5290, 5634, 6867
Eco147I AGGCCT 1 cut(s) 4311
Eco24I GRGCYC 2 cut(s) 5222, 6523
Eco31I GGTCTC 5 cut(s) 1394, 2035, 3390, 3627, 3926
Eco32I GATATC 3 cut(s) 3603, 4091, 6275
Eco53kI GAGCTC 1 cut(s) 5220
Eco57I CTGAAG 8 cut(s) 4028, 4545, 4551, 4927, 5048, 5067, 5097, 6714
Eco81I CCTNAGG 1 cut(s) 3680
Eco88I CYCGRG 3 cut(s) 1452, 2194, 3353
Eco91I GGTNACC 2 cut(s) 6545, 7428
EcoICRI GAGCTC 1 cut(s) 5220
EcoNI CCTNNNNNAGG 3 cut(s) 1592, 3000, 7327
EcoO109I RGGNCCY 2 cut(s) 4359, 6817
EcoO65I GGTNACC 2 cut(s) 6545, 7428
EcoRI GAATTC 3 cut(s) 4267, 5401, 6204
EcoRII CCWGG 5 cut(s) 1258, 1495, 2626, 4650, 6854
EcoRV GATATC 3 cut(s) 3603, 4091, 6275
EcoT14I CCWWGG 7 cut(s) 1305, 1768, 3063, 3766, 5290, 5634, 6867
EcoT22I ATGCAT 2 cut(s) 2455, 5839
EcoT38I GRGCYC 2 cut(s) 5222, 6523
ErhI CCWWGG 7 cut(s) 1305, 1768, 3063, 3766, 5290, 5634, 6867
FalI AAGNNNNNCTT 4 cut(s) 3214, 3246, 4775, 4807
FauI CCCGC 1 cut(s) 11
FauNDI CATATG 6 cut(s) 985, 1066, 1223, 2149, 4871, 5839
FbaI TGATCA 1 cut(s) 4708
FblI GTMKAC 5 cut(s) 2031, 2608, 3255, 5196, 5255
FriOI GRGCYC 2 cut(s) 5222, 6523
FspI TGCGCA 2 cut(s) 1442, 3851
GlaI GCGC 8 cut(s) 1442, 1571, 2126, 3786, 3851, 5462, 6289, 7057
GsaI CCCAGC 1 cut(s) 5760
GsuI CTGGAG 8 cut(s) 85, 966, 3391, 5341, 5526, 5697, 6147, 6395
HaeII RGCGCY 1 cut(s) 2128
HaeIII GGCC 8 cut(s) 1421, 2499, 2950, 3316, 4311, 5470, 7157, 7260
HapII CCGG 6 cut(s) 958, 2681, 3567, 6033, 6229, 7460
HgaI GACGC 2 cut(s) 58, 7417
HhaI GCGC 8 cut(s) 1443, 1572, 2127, 3787, 3852, 5463, 6290, 7058
Hin1I GRCGYC 2 cut(s) 4722, 6726
Hin6I GCGC 8 cut(s) 1441, 1570, 2125, 3785, 3850, 5461, 6288, 7056
HinP1I GCGC 8 cut(s) 1441, 1570, 2125, 3785, 3850, 5461, 6288, 7056
HincII GTYRAC 2 cut(s) 4531, 4681
HindII GTYRAC 2 cut(s) 4531, 4681
HindIII AAGCTT 7 cut(s) 2259, 2526, 3131, 3223, 4693, 5383, 6391
HpaI GTTAAC 1 cut(s) 4681
HpaII CCGG 6 cut(s) 958, 2681, 3567, 6033, 6229, 7460
Hpy99I CGWCG 1 cut(s) 6556
HpyCH4IV ACGT 6 cut(s) 1163, 3304, 3610, 3945, 4722, 6726
HpySE526I ACGT 6 cut(s) 1163, 3304, 3610, 3945, 4722, 6726
Hsp92I GRCGYC 2 cut(s) 4722, 6726
HspAI GCGC 8 cut(s) 1441, 1570, 2125, 3785, 3850, 5461, 6288, 7056
KflI GGGWCCC 1 cut(s) 6817
Kpn2I TCCGGA 2 cut(s) 957, 3566
Ksp22I TGATCA 1 cut(s) 4708
KspAI GTTAAC 1 cut(s) 4681
LguI GCTCTTC 1 cut(s) 2805
MabI ACCWGGT 1 cut(s) 1495
MaeII ACGT 6 cut(s) 1163, 3304, 3610, 3945, 4722, 6726
MbiI CCGCTC 2 cut(s) 249, 950
MfeI CAATTG 6 cut(s) 1425, 1800, 1956, 2154, 4116, 5516
MflI RGATCY 5 cut(s) 911, 2427, 3709, 4857, 6913
MhlI GDGCHC 4 cut(s) 4734, 5222, 6322, 6523
MlsI TGGCCA 3 cut(s) 1421, 5470, 7157
MluNI TGGCCA 3 cut(s) 1421, 5470, 7157
MmeI TCCRAC 6 cut(s) 487, 2352, 2748, 4842, 6220, 7011
Mox20I TGGCCA 3 cut(s) 1421, 5470, 7157
Mph1103I ATGCAT 2 cut(s) 2455, 5839
MroI TCCGGA 2 cut(s) 957, 3566
MscI TGGCCA 3 cut(s) 1421, 5470, 7157
MslI CAYNNNNRTG 9 cut(s) 630, 930, 1767, 3248, 3420, 4028, 4173, 5972, 6120
Msp20I TGGCCA 3 cut(s) 1421, 5470, 7157
MspA1I CMGCKG 2 cut(s) 50, 5720
MspI CCGG 6 cut(s) 958, 2681, 3567, 6033, 6229, 7460
MspR9I CCNGG 6 cut(s) 1260, 1497, 2628, 2682, 4652, 6856
MunI CAATTG 6 cut(s) 1425, 1800, 1956, 2154, 4116, 5516
Mva1269I GAATGC 4 cut(s) 886, 1187, 3244, 3507
MvaI CCWGG 5 cut(s) 1260, 1497, 2628, 4652, 6856
MvnI CGCG 3 cut(s) 3499, 5127, 6288
NciI CCSGG 1 cut(s) 2682
NcoI CCATGG 2 cut(s) 1768, 5290
NdeI CATATG 6 cut(s) 985, 1066, 1223, 2149, 4871, 5839
NheI GCTAGC 4 cut(s) 43, 1157, 3718, 5579
NmeAIII GCCGAG 1 cut(s) 4126
NmuCI GTSAC 7 cut(s) 4286, 4865, 5668, 5703, 7003, 7199, 7428
NruI TCGCGA 1 cut(s) 5127
NsbI TGCGCA 2 cut(s) 1442, 3851
NsiI ATGCAT 2 cut(s) 2455, 5839
NspI RCATGY 6 cut(s) 2739, 3279, 3661, 6125, 6389, 7441
NspV TTCGAA 1 cut(s) 3645
PaeI GCATGC 1 cut(s) 2739
PaeR7I CTCGAG 1 cut(s) 3353
PagI TCATGA 3 cut(s) 285, 4711, 5533
PceI AGGCCT 1 cut(s) 4311
PciI ACATGT 1 cut(s) 3275
PciSI GCTCTTC 1 cut(s) 2805
PctI GAATGC 4 cut(s) 886, 1187, 3244, 3507
PflMI CCANNNNNTGG 2 cut(s) 2371, 3706
PfoI TCCNGGA 1 cut(s) 2626
Ppu21I YACGTR 3 cut(s) 1164, 3305, 3946
PpuMI RGGWCCY 2 cut(s) 4359, 6817
PscI ACATGT 1 cut(s) 3275
PshBI ATTAAT 3 cut(s) 1629, 2859, 6261
PsiI TTATAA 1 cut(s) 3969
Psp124BI GAGCTC 1 cut(s) 5222
Psp5II RGGWCCY 2 cut(s) 4359, 6817
Psp6I CCWGG 5 cut(s) 1258, 1495, 2626, 4650, 6854
PspEI GGTNACC 2 cut(s) 6545, 7428
PspFI CCCAGC 1 cut(s) 5756
PspGI CCWGG 5 cut(s) 1258, 1495, 2626, 4650, 6854
PspPPI RGGWCCY 2 cut(s) 4359, 6817
PspXI VCTCGAGB 1 cut(s) 3353
PstI CTGCAG 4 cut(s) 4300, 4624, 4848, 6183
PstNI CAGNNNCTG 3 cut(s) 3459, 5071, 5720
PsuI RGATCY 5 cut(s) 911, 2427, 3709, 4857, 6913
PvuII CAGCTG 2 cut(s) 50, 5720
RruI TCGCGA 1 cut(s) 5127
RseI CAYNNNNRTG 9 cut(s) 630, 930, 1767, 3248, 3420, 4028, 4173, 5972, 6120
Rsr2I CGGWCCG 1 cut(s) 4815
RsrII CGGWCCG 1 cut(s) 4815
SacI GAGCTC 1 cut(s) 5222
SapI GCTCTTC 1 cut(s) 2805
ScaI AGTACT 6 cut(s) 1381, 2785, 3359, 3467, 4330, 6760
ScrFI CCNGG 6 cut(s) 1260, 1497, 2628, 2682, 4652, 6856
SduI GDGCHC 4 cut(s) 4734, 5222, 6322, 6523
SexAI ACCWGGT 1 cut(s) 1495
SfcI CTRYAG 8 cut(s) 1383, 2176, 4296, 4620, 4844, 5256, 5272, 6179
Sfr274I CTCGAG 1 cut(s) 3353
SfuI TTCGAA 1 cut(s) 3645
SlaI CTCGAG 1 cut(s) 3353
SmiMI CAYNNNNRTG 9 cut(s) 630, 930, 1767, 3248, 3420, 4028, 4173, 5972, 6120
SmlI CTYRAG 6 cut(s) 201, 2182, 2707, 3103, 3191, 3353
SmoI CTYRAG 6 cut(s) 201, 2182, 2707, 3103, 3191, 3353
SnaBI TACGTA 2 cut(s) 3305, 3946
SpeI ACTAGT 1 cut(s) 6242
SphI GCATGC 1 cut(s) 2739
SseBI AGGCCT 1 cut(s) 4311
SspI AATATT 2 cut(s) 2857, 3035
SstI GAGCTC 1 cut(s) 5222
StuI AGGCCT 1 cut(s) 4311
StyD4I CCNGG 6 cut(s) 1258, 1495, 2626, 2680, 4650, 6854
StyI CCWWGG 7 cut(s) 1305, 1768, 3063, 3766, 5290, 5634, 6867
TaiI ACGT 6 cut(s) 1166, 3307, 3613, 3948, 4725, 6729
TaqII GACCGA 3 cut(s) 4803, 6792, 7165
TatI WGTACW 8 cut(s) 1379, 1902, 2783, 3357, 3465, 4328, 6534, 6758
TauI GCSGC 3 cut(s) 151, 4973, 7263
TseFI GTSAC 7 cut(s) 4286, 4865, 5668, 5703, 7003, 7199, 7428
Tsp45I GTSAC 7 cut(s) 4286, 4865, 5668, 5703, 7003, 7199, 7428
TspGWI ACGGA 8 cut(s) 878, 1094, 2657, 4798, 4853, 5884, 6131, 6989
Van91I CCANNNNNTGG 2 cut(s) 2371, 3706
VneI GTGCAC 1 cut(s) 6318
VspI ATTAAT 3 cut(s) 1629, 2859, 6261
XagI CCTNNNNNAGG 3 cut(s) 1592, 3000, 7327
XapI RAATTY 8 cut(s) 755, 2375, 3155, 4267, 4999, 5401, 5736, 6204
XbaI TCTAGA 3 cut(s) 817, 908, 2695
XceI RCATGY 6 cut(s) 2739, 3279, 3661, 6125, 6389, 7441
XcmI CCANNNNNNNNNTGG 1 cut(s) 6122
XhoI CTCGAG 1 cut(s) 3353
XmiI GTMKAC 5 cut(s) 2031, 2608, 3255, 5196, 5255
ZraI GACGTC 2 cut(s) 4723, 6727
ZrmI AGTACT 6 cut(s) 1381, 2785, 3359, 3467, 4330, 6760
Zsp2I ATGCAT 2 cut(s) 2455, 5839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.