AT3G02550

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014406
Physical Location & Seq
Reverse (-)
536500 .. 538126
1627 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02550.1

Sequence Viewer

Length: 792 bp
ATGCGGATGAGCTGTAATGGATGCAGAGTTCTTCGGAAAGGGTGTAGTGAGGATTGTAGTATAAGGCCGTGTTTGGCTTGGATCAAATCGCCTGAAGCGCAAGCTAACGCAACTGTCTTTCTCGCCAAGTTCTATGGCCGTGCTGGACTCATGAACCTCATCAACGCCGGTCCCAATCACCTTCGTCCTGGGATTTTCCGATCGTTGTTGCACGAAGCTTGTGGGAGGATTGTGAATCCGATCTATGGTTCGGTGGGTTTGTTGTGGTCGGGGAATTGGCAGCTTTGTCAAGACGCCGTGGAGGCTGTGATGAAAGGAGAACCGGTCAAAGAGATCGCCACAGACGCTGCGACGATCGGCCAAGGTCCGCCTCTTAAGATCTACGACATCCGACATATCTCCAAGGATGATAACTCTGCCGCCGCGGCTACTGGCTCAACCGATTTGAAACTTGCGAAAACTCGCCGTGCTAAGCGGGTCTCCACCGTCGCGATACAGGCGGAATCGGAGGGAAAGTCTGACGAGGCTAGTCACGATTCGTCGTTGAGTCATCAGTCTGAAATAGTGGCTGCTCATGAAGGAGAGAGCAAGGAATCCGAGAGCAATGTCTCTGAGGTTTTGGCATTCTCGCCTCCGGCTGTGAAGGGCTCCGGCGAGATAAAGCTTGACCTAACTTTAAGGCTCGAACCGGTGTCACGTGCGTATCATGTGGTACCTGTTAAGAAGAGAAGGATCGGCGTGTTTGGCACGTGTCAGAAGGAGAGCACGTGTAAGACTGAGCTTATGCTCTAA

Protein Analysis

263

Amino Acids

28.42

Weight (kDa)

8.7

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 4 - 103 6.8e-22 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02550
fragaria_vesca FvH4_6g45150
malus_domestica MD09G1088700.v1.1 MD17G1078600.v1.1
prunus_persica Prupe.3G236200_v2.0.a1
pyrus_communis pycom09g01450 pycom17g07790
rosa_chinensis RchiOBHm_Chr2g0162621
rosa_laevigata RLG00000021353
rosa_multiflora Rmu_ssc0000364.1_g000001
rosa_roxburghii Rroxscaffold_2G00087820
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564500 Rh2BG577400 Rh2CG547100 Rh2DG587000
rosa_wichuraiana Rw2G046700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 712
AccII CGCG 2 cut(s) 425, 491
AciI CCGC 7 cut(s) 4, 368, 420, 423, 425, 475, 500
AclWI GGATC 2 cut(s) 89, 740
AcoI YGGCCR 2 cut(s) 136, 358
AcuI CTGAAG 1 cut(s) 114
AcvI CACGTG 3 cut(s) 698, 750, 768
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 1 cut(s) 245
AflII CTTAAG 1 cut(s) 374
AflIII ACRYGT 2 cut(s) 749, 767
AgeI ACCGGT 2 cut(s) 322, 688
AgsI TTSAA 1 cut(s) 448
AjnI CCWGG 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 12, 44
AluBI AGCT 6 cut(s) 12, 104, 218, 283, 664, 781
AluI AGCT 6 cut(s) 12, 104, 218, 283, 664, 781
Alw21I GWGCWC 1 cut(s) 767
Alw26I GTCTC 2 cut(s) 484, 613
AlwI GGATC 2 cut(s) 89, 740
AlwNI CAGNNNCTG 1 cut(s) 347
AoxI GGCC 3 cut(s) 65, 136, 358
ApeKI GCWGC 3 cut(s) 280, 347, 569
AsiGI ACCGGT 2 cut(s) 322, 688
Asp718I GGTACC 1 cut(s) 712
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 170, 365
AsuHPI GGTGA 1 cut(s) 170
AvaII GGWCC 2 cut(s) 170, 365
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BanI GGYRCC 1 cut(s) 712
BanII GRGCYC 1 cut(s) 650
BbrPI CACGTG 3 cut(s) 698, 750, 768
Bbv12I GWGCWC 1 cut(s) 767
BbvI GCAGC 3 cut(s) 292, 334, 556
BceAI ACGGC 4 cut(s) 52, 123, 281, 450
BciT130I CCWGG 1 cut(s) 189
BcoDI GTCTC 2 cut(s) 484, 613
BfaI CTAG 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 374
BglI GCCNNNNNGGC 2 cut(s) 302, 425
BglII AGATCT 1 cut(s) 378
BisI GCNGC 6 cut(s) 281, 348, 420, 423, 426, 570
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 6 cut(s) 282, 349, 421, 424, 427, 571
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 2 cut(s) 170, 365
BmgT120I GGNCC 2 cut(s) 170, 365
BmiI GGNNCC 3 cut(s) 172, 649, 714
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 1 cut(s) 11
Bpu1102I GCTNAGC 1 cut(s) 471
BsaAI YACGTR 3 cut(s) 698, 750, 768
BsaHI GRCGYC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 484
BsaJI CCNNGG 5 cut(s) 188, 297, 361, 402, 423
BsaWI WCCGGW 2 cut(s) 322, 688
BsaXI ACNNNNNCTCC 6 cut(s) 309, 339, 500, 530, 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 3 cut(s) 167, 322, 688
Bse1I ACTGG 1 cut(s) 436
Bse3DI GCAATG 1 cut(s) 610
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 5 cut(s) 188, 297, 361, 402, 423
BseGI GGATG 4 cut(s) 12, 26, 387, 412
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 2 cut(s) 603, 768
BseNI ACTGG 1 cut(s) 436
BseXI GCAGC 3 cut(s) 292, 334, 556
Bsh1236I CGCG 2 cut(s) 425, 491
Bsh1285I CGRYCG 2 cut(s) 203, 357
BshFI GGCC 3 cut(s) 67, 138, 360
BshNI GGYRCC 1 cut(s) 712
BshTI ACCGGT 2 cut(s) 322, 688
BsiEI CGRYCG 2 cut(s) 203, 357
BsiHKAI GWGCWC 1 cut(s) 767
BsiSI CCGG 5 cut(s) 168, 323, 635, 651, 689
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 484, 613
BsmFI GGGAC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 3 cut(s) 67, 138, 360
Bso31I GGTCTC 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 650, 767
Bsp143I GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp68I TCGCGA 1 cut(s) 491
BspACI CCGC 7 cut(s) 4, 368, 420, 423, 425, 475, 500
BspANI GGCC 3 cut(s) 67, 138, 360
BspCNI CTCAG 2 cut(s) 604, 769
BspFNI CGCG 2 cut(s) 425, 491
BspHI TCATGA 2 cut(s) 150, 574
BspLI GGNNCC 3 cut(s) 172, 649, 714
BspPI GGATC 2 cut(s) 89, 740
BspT107I GGYRCC 1 cut(s) 712
BspTI CTTAAG 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 3 cut(s) 167, 322, 688
BsrI ACTGG 1 cut(s) 436
BssAI RCCGGY 3 cut(s) 167, 322, 688
BssECI CCNNGG 5 cut(s) 188, 297, 361, 402, 423
BssMI GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 2 cut(s) 361, 402
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 115, 487
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 1 cut(s) 294
BstAFI CTTAAG 1 cut(s) 374
BstBAI YACGTR 3 cut(s) 698, 750, 768
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 3 cut(s) 471, 612, 777
BstDSI CCRYGG 2 cut(s) 297, 423
BstF5I GGATG 4 cut(s) 12, 26, 387, 412
BstFNI CGCG 2 cut(s) 425, 491
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 7 cut(s) 84, 203, 243, 336, 357, 381, 735
BstMAI GTCTC 2 cut(s) 484, 613
BstMBI GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
BstMCI CGRYCG 2 cut(s) 203, 357
BstMWI GCNNNNNNNGC 6 cut(s) 97, 302, 344, 425, 497, 744
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstUI CGCG 2 cut(s) 425, 491
BstV1I GCAGC 3 cut(s) 292, 334, 556
BstX2I RGATCY 1 cut(s) 378
BstYI RGATCY 1 cut(s) 378
BsuRI GGCC 3 cut(s) 67, 138, 360
BtgI CCRYGG 2 cut(s) 297, 423
BtsCI GGATG 4 cut(s) 12, 26, 387, 412
BtuMI TCGCGA 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 347
CciI TCATGA 2 cut(s) 150, 574
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 3 cut(s) 167, 322, 688
Cfr13I GGNCC 2 cut(s) 170, 365
Cfr42I CCGCGG 1 cut(s) 426
CseI GACGC 2 cut(s) 302, 353
Csp6I GTAC 1 cut(s) 713
CspAI ACCGGT 2 cut(s) 322, 688
CviAII CATG 3 cut(s) 151, 575, 707
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 3 cut(s) 471, 612, 777
DpnI GATC 7 cut(s) 83, 202, 242, 335, 356, 380, 734
DpnII GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
EaeI YGGCCR 2 cut(s) 136, 358
Eam1104I CTCTTC 1 cut(s) 719
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 2 cut(s) 357, 515
Eco130I CCWWGG 2 cut(s) 361, 402
Eco24I GRGCYC 1 cut(s) 650
Eco31I GGTCTC 1 cut(s) 484
Eco47I GGWCC 2 cut(s) 170, 365
Eco57I CTGAAG 1 cut(s) 114
Eco72I CACGTG 3 cut(s) 698, 750, 768
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 361, 402
EcoT38I GRGCYC 1 cut(s) 650
ErhI CCWWGG 2 cut(s) 361, 402
FaeI CATG 3 cut(s) 154, 578, 710
FaiI YATR 8 cut(s) 62, 135, 152, 246, 396, 576, 708, 785
FaqI GGGAC 1 cut(s) 156
FatI CATG 3 cut(s) 150, 574, 706
FauI CCCGC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 281, 348, 420, 423, 426, 570
FokI GGATG 4 cut(s) 19, 33, 374, 419
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 6 cut(s) 281, 348, 420, 423, 426, 570
FspBI CTAG 1 cut(s) 528
GlaI GCGC 1 cut(s) 99
GluI GCNGC 6 cut(s) 281, 348, 420, 423, 426, 570
HaeIII GGCC 3 cut(s) 67, 138, 360
HapII CCGG 5 cut(s) 168, 323, 635, 651, 689
HgaI GACGC 2 cut(s) 302, 353
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 3 cut(s) 154, 578, 710
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HindIII AAGCTT 2 cut(s) 216, 662
HinfI GANTC 6 cut(s) 147, 235, 503, 536, 547, 593
HpaII CCGG 5 cut(s) 168, 323, 635, 651, 689
HphI GGTGA 1 cut(s) 170
Hpy188III TCNNGA 5 cut(s) 151, 290, 490, 533, 575
Hpy99I CGWCG 3 cut(s) 355, 491, 544
HpyAV CCTTC 5 cut(s) 191, 572, 637, 723, 751
HpyCH4III ACNGT 2 cut(s) 115, 487
HpyCH4IV ACGT 3 cut(s) 697, 749, 767
HpyCH4V TGCA 2 cut(s) 24, 211
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 302, 344, 425, 497, 744
HpyF3I CTNAG 3 cut(s) 471, 612, 777
HpySE526I ACGT 3 cut(s) 697, 749, 767
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 3 cut(s) 154, 578, 710
HspAI GCGC 1 cut(s) 98
KpnI GGTACC 1 cut(s) 716
KspI CCGCGG 1 cut(s) 426
Kzo9I GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
LmnI GCTCC 1 cut(s) 653
Lsp1109I GCAGC 3 cut(s) 292, 334, 556
LweI GCATC 1 cut(s) 11
MaeI CTAG 1 cut(s) 528
MaeII ACGT 3 cut(s) 697, 749, 767
MaeIII GTNAC 2 cut(s) 530, 693
MalI GATC 7 cut(s) 83, 202, 242, 335, 356, 380, 734
MboI GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
MboII GAAGA 2 cut(s) 23, 736
MflI RGATCY 1 cut(s) 378
MhlI GDGCHC 2 cut(s) 650, 767
MluCI AATT 1 cut(s) 274
MlyI GAGTC 2 cut(s) 141, 556
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 9 cut(s) 43, 167, 219, 295, 381, 502, 517, 607, 642
MseI TTAA 3 cut(s) 375, 677, 720
MspA1I CMGCKG 1 cut(s) 425
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 5 cut(s) 168, 323, 635, 651, 689
MspR9I CCNGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 2 cut(s) 425, 491
MwoI GCNNNNNNNGC 6 cut(s) 97, 302, 344, 425, 497, 744
NdeII GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
NlaIII CATG 3 cut(s) 154, 578, 710
NlaIV GGNNCC 3 cut(s) 172, 649, 714
NmuCI GTSAC 2 cut(s) 530, 693
NruI TCGCGA 1 cut(s) 491
PagI TCATGA 2 cut(s) 150, 574
PctI GAATGC 1 cut(s) 623
PfeI GAWTC 4 cut(s) 235, 503, 536, 593
PinAI ACCGGT 2 cut(s) 322, 688
PkrI GCNGC 6 cut(s) 282, 349, 421, 424, 427, 571
Ple19I CGATCG 2 cut(s) 203, 357
PleI GAGTC 2 cut(s) 141, 555
PmaCI CACGTG 3 cut(s) 698, 750, 768
PmlI CACGTG 3 cut(s) 698, 750, 768
PpsI GAGTC 2 cut(s) 141, 555
Ppu21I YACGTR 3 cut(s) 698, 750, 768
Psp6I CCWGG 1 cut(s) 187
PspCI CACGTG 3 cut(s) 698, 750, 768
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 172, 649, 714
PspPI GGNCC 2 cut(s) 170, 365
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 378
PvuI CGATCG 2 cut(s) 203, 357
RruI TCGCGA 1 cut(s) 491
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
SacII CCGCGG 1 cut(s) 426
SaqAI TTAA 3 cut(s) 375, 677, 720
SatI GCNGC 6 cut(s) 281, 348, 420, 423, 426, 570
Sau3AI GATC 7 cut(s) 81, 200, 240, 333, 354, 378, 732
Sau96I GGNCC 2 cut(s) 170, 365
SchI GAGTC 2 cut(s) 141, 556
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 2 cut(s) 650, 767
SfaNI GCATC 1 cut(s) 11
Sfr303I CCGCGG 1 cut(s) 426
SgrBI CCGCGG 1 cut(s) 426
SinI GGWCC 2 cut(s) 170, 365
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 1 cut(s) 274
SsiI CCGC 7 cut(s) 4, 368, 420, 423, 425, 475, 500
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 2 cut(s) 361, 402
TaaI ACNGT 2 cut(s) 115, 487
TaiI ACGT 3 cut(s) 700, 752, 770
TaqI TCGA 1 cut(s) 684
TasI AATT 1 cut(s) 274
TauI GCSGC 3 cut(s) 422, 425, 428
TfiI GAWTC 4 cut(s) 235, 503, 536, 593
Tru1I TTAA 3 cut(s) 375, 677, 720
Tru9I TTAA 3 cut(s) 375, 677, 720
TseFI GTSAC 2 cut(s) 530, 693
TseI GCWGC 3 cut(s) 280, 347, 569
Tsp45I GTSAC 2 cut(s) 530, 693
TspDTI ATGAA 3 cut(s) 167, 326, 591
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 170, 365
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.