MD09G1088700.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014406
Physical Location & Seq
Forward (+)
6468692 .. 6470254
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1088700.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCGGATGAGCTGTAATGGATGCCGAATACTCCGAAAGGGCTGCAGCGAAAATTGCAGCATCCGGCCTTGCCTGCAGTGGATTAAGAGCCCGGAAGCCCAAGCAAACGCCACCGTTTTCCTCGCCAAGTTCTACGGCCGCGCCGGACTCATGAACCTCGTCAACGCCGGCCCCGAACATCTCCGCCCTGCGATCTTTCGGTCTTTGCTATACGAGGCATGCGGACGGATTGTGAACCCGATTTACGGGTCGGTCGGGTTGTTGTGGTCCGGGAGCTGGCAGCTCTGCCAAGCCGCCGTTGAAGCCGTGCTGAAAGGCCAGCCGATTACGCCGATTACTTCCGAAGCGGCGGCGGACGGGCATGGCCCGCCTCTCAAGGCCTACGACATTCGCCATGTGTCCAAGGACGAGAACTCGGCCGCGTCCAACGATCCTCAGAAGGTTAAGACTCGGTACCGGTTCAAGCGGTCCGTTGTCAAGCCCAAGACCAACAAAGTAGGCTCCGGTTCTGGGTCGGGCTGTGGCTCAGGAGCTGATGACTCGGCCAAGCCGAGTTGTACCGGGTACTGTGGGAACGAGTTTAACCGATCGACGAGTCACGAGTCGTCGCTGAGTCACCAGTCCGAGGTGGCTGCCAACGTGGACGGCGACAGCAAGGAATCGGAGAGCATGATTTCGTCCGAGACGGCTGAGGCTGAACTCTTTTCCCGAGCCGAGCCACAATCGGCTCGCAAGCGGAGGGAGCCGGCTCAGGATGGGGAGCTAGCTTTAGAGCTGACGCTTGGGTTGGAGCCGCCATCACGGGCCCACCACGTCGTTCCGGTGAAGAAGAGAAGAATTGAGGCTGAATTCGGCAGCTTATCATCGGCCGGCGACGGCGCGTGTAAAATGGAGCTGGGGCTCGATTTCGTGGCCTGA

Protein Analysis

306

Amino Acids

32.72

Weight (kDa)

8.45

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 4 - 103 2.7e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02550
fragaria_vesca FvH4_6g45150
malus_domestica MD09G1088700.v1.1 MD17G1078600.v1.1
prunus_persica Prupe.3G236200_v2.0.a1
pyrus_communis pycom09g01450 pycom17g07790
rosa_chinensis RchiOBHm_Chr2g0162621
rosa_laevigata RLG00000021353
rosa_multiflora Rmu_ssc0000364.1_g000001
rosa_roxburghii Rroxscaffold_2G00087820
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564500 Rh2BG577400 Rh2CG547100 Rh2DG587000
rosa_wichuraiana Rw2G046700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 453
AccII CGCG 3 cut(s) 141, 422, 881
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 4 cut(s) 136, 417, 543, 867
AcsI RAATTY 1 cut(s) 848
AfaI GTAC 3 cut(s) 455, 559, 566
AfiI CCNNNNNNNGG 4 cut(s) 245, 276, 510, 625
AgeI ACCGGT 1 cut(s) 456
AgsI TTSAA 2 cut(s) 302, 463
AjiI CACGTC 1 cut(s) 814
AluBI AGCT 9 cut(s) 12, 276, 283, 533, 763, 767, 775, 858, 895
AluI AGCT 9 cut(s) 12, 276, 283, 533, 763, 767, 775, 858, 895
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 708
ApaI GGGCCC 1 cut(s) 808
ApeKI GCWGC 6 cut(s) 42, 45, 57, 280, 632, 855
ApoI RAATTY 1 cut(s) 848
AsiGI ACCGGT 1 cut(s) 456
Asp718I GGTACC 1 cut(s) 453
AspLEI GCGC 2 cut(s) 143, 881
AspS9I GGNCC 6 cut(s) 170, 267, 365, 468, 804, 805
AsuC2I CCSGG 3 cut(s) 92, 271, 562
AsuHPI GGTGA 2 cut(s) 608, 835
AsuNHI GCTAGC 1 cut(s) 763
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 267, 468
BaeGI GKGCMC 1 cut(s) 808
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 3 cut(s) 92, 808, 903
BauI CACGAG 1 cut(s) 599
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 6 cut(s) 29, 57, 69, 292, 619, 867
BccI CCATC 2 cut(s) 749, 805
BceAI ACGGC 6 cut(s) 151, 281, 290, 661, 702, 892
BcgI CGANNNNNNTGC 4 cut(s) 24, 58, 614, 648
BcnI CCSGG 3 cut(s) 92, 271, 562
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 2 cut(s) 43, 74
Bme1390I CCNGG 3 cut(s) 92, 271, 562
Bme18I GGWCC 2 cut(s) 267, 468
BmeT110I CYCGRG 1 cut(s) 708
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 6 cut(s) 170, 267, 365, 468, 804, 805
BmiI GGNNCC 6 cut(s) 172, 455, 502, 744, 792, 806
BmrFI CCNGG 3 cut(s) 92, 271, 562
BmsI GCATC 2 cut(s) 11, 69
BmtI GCTAGC 1 cut(s) 767
Bpu10I CCTNAGC 3 cut(s) 526, 690, 750
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 3 cut(s) 92, 271, 562
BsaJI CCNNGG 2 cut(s) 402, 624
BsaWI WCCGGW 3 cut(s) 456, 503, 820
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 276, 510, 625
Bse118I RCCGGY 4 cut(s) 167, 456, 745, 869
Bse1I ACTGG 1 cut(s) 619
BseDI CCNNGG 2 cut(s) 402, 624
BseGI GGATG 4 cut(s) 12, 26, 60, 760
BseLI CCNNNNNNNGG 4 cut(s) 245, 276, 510, 625
BseMII CTCAG 5 cut(s) 449, 540, 602, 681, 764
BseNI ACTGG 1 cut(s) 619
BseSI GKGCMC 1 cut(s) 808
BseX3I CGGCCG 3 cut(s) 136, 417, 867
BseXI GCAGC 6 cut(s) 29, 57, 69, 292, 619, 867
BseYI CCCAGC 1 cut(s) 895
Bsh1236I CGCG 3 cut(s) 141, 422, 881
Bsh1285I CGRYCG 5 cut(s) 139, 255, 420, 590, 870
BshNI GGYRCC 1 cut(s) 453
BshTI ACCGGT 1 cut(s) 456
BsiEI CGRYCG 5 cut(s) 139, 255, 420, 590, 870
BsiHKCI CYCGRG 1 cut(s) 708
BslI CCNNNNNNNGG 4 cut(s) 245, 276, 510, 625
BsmAI GTCTC 1 cut(s) 677
BsmBI CGTCTC 1 cut(s) 677
BsoBI CYCGRG 1 cut(s) 708
Bsp120I GGGCCC 1 cut(s) 804
Bsp1286I GDGCHC 3 cut(s) 92, 808, 903
Bsp143I GATC 3 cut(s) 192, 430, 587
BspCNI CTCAG 5 cut(s) 448, 539, 603, 682, 763
BspFNI CGCG 3 cut(s) 141, 422, 881
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 6 cut(s) 172, 455, 502, 744, 792, 806
BspMAI CTGCAG 2 cut(s) 47, 78
BspOI GCTAGC 1 cut(s) 767
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 453
BsrFI RCCGGY 4 cut(s) 167, 456, 745, 869
BsrI ACTGG 1 cut(s) 619
BssAI RCCGGY 4 cut(s) 167, 456, 745, 869
BssECI CCNNGG 2 cut(s) 402, 624
BssMI GATC 3 cut(s) 192, 430, 587
BssSI CACGAG 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 115, 569
Bst6I CTCTTC 1 cut(s) 824
BstDEI CTNAG 5 cut(s) 435, 526, 611, 690, 750
BstF5I GGATG 4 cut(s) 12, 26, 60, 760
BstFNI CGCG 3 cut(s) 141, 422, 881
BstHHI GCGC 2 cut(s) 143, 881
BstKTI GATC 3 cut(s) 195, 433, 590
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 3 cut(s) 192, 430, 587
BstMCI CGRYCG 5 cut(s) 139, 255, 420, 590, 870
BstMWI GCNNNNNNNGC 6 cut(s) 54, 73, 302, 328, 367, 742
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 3 cut(s) 90, 269, 560
BstSFI CTRYAG 2 cut(s) 43, 74
BstSLI GKGCMC 1 cut(s) 808
BstUI CGCG 3 cut(s) 141, 422, 881
BstV1I GCAGC 6 cut(s) 29, 57, 69, 292, 619, 867
BstZI CGGCCG 3 cut(s) 136, 417, 867
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 4 cut(s) 12, 26, 60, 760
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 150
CfoI GCGC 2 cut(s) 143, 881
Cfr10I RCCGGY 4 cut(s) 167, 456, 745, 869
Cfr13I GGNCC 6 cut(s) 170, 267, 365, 468, 804, 805
CpoI CGGWCCG 1 cut(s) 468
CseI GACGC 2 cut(s) 411, 787
Csp6I GTAC 3 cut(s) 454, 558, 565
CspAI ACCGGT 1 cut(s) 456
CspI CGGWCCG 1 cut(s) 468
CviAII CATG 5 cut(s) 151, 219, 362, 395, 670
CviQI GTAC 3 cut(s) 454, 558, 565
DdeI CTNAG 5 cut(s) 435, 526, 611, 690, 750
DpnI GATC 3 cut(s) 194, 432, 589
DpnII GATC 3 cut(s) 192, 430, 587
EaeI YGGCCR 4 cut(s) 136, 417, 543, 867
EagI CGGCCG 3 cut(s) 136, 417, 867
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
EciI GGCGGA 2 cut(s) 173, 368
EclXI CGGCCG 3 cut(s) 136, 417, 867
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 3 cut(s) 92, 808, 903
Eco47I GGWCC 2 cut(s) 267, 468
Eco52I CGGCCG 3 cut(s) 136, 417, 867
Eco88I CYCGRG 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 848
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 3 cut(s) 92, 808, 903
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 5 cut(s) 154, 222, 365, 398, 673
FaiI YATR 6 cut(s) 152, 211, 220, 363, 396, 671
FatI CATG 5 cut(s) 150, 218, 361, 394, 669
FauI CCCGC 1 cut(s) 375
FokI GGATG 4 cut(s) 19, 33, 47, 767
FriOI GRGCYC 3 cut(s) 92, 808, 903
FspBI CTAG 1 cut(s) 764
GlaI GCGC 2 cut(s) 142, 880
GsaI CCCAGC 1 cut(s) 899
HgaI GACGC 2 cut(s) 411, 787
HhaI GCGC 2 cut(s) 143, 881
Hin1II CATG 5 cut(s) 154, 222, 365, 398, 673
Hin6I GCGC 2 cut(s) 141, 879
HinP1I GCGC 2 cut(s) 141, 879
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 7 cut(s) 147, 448, 539, 595, 602, 613, 659
HphI GGTGA 2 cut(s) 608, 835
Hpy166II GTNNAC 3 cut(s) 163, 235, 643
Hpy188I TCNGA 6 cut(s) 35, 343, 438, 625, 664, 682
Hpy188III TCNNGA 5 cut(s) 151, 528, 599, 708, 752
Hpy8I GTNNAC 3 cut(s) 163, 235, 643
Hpy99I CGWCG 4 cut(s) 595, 610, 818, 878
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 2 cut(s) 115, 569
HpyCH4IV ACGT 2 cut(s) 639, 813
HpyCH4V TGCA 3 cut(s) 45, 57, 76
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 73, 302, 328, 367, 742
HpyF3I CTNAG 5 cut(s) 435, 526, 611, 690, 750
HpySE526I ACGT 2 cut(s) 639, 813
Hsp92II CATG 5 cut(s) 154, 222, 365, 398, 673
HspAI GCGC 2 cut(s) 141, 879
KpnI GGTACC 1 cut(s) 457
KroI GCCGGC 3 cut(s) 167, 745, 869
KroNI GCCGGC 3 cut(s) 169, 747, 871
Kzo9I GATC 3 cut(s) 192, 430, 587
LmnI GCTCC 7 cut(s) 273, 506, 530, 742, 760, 790, 892
Lsp1109I GCAGC 6 cut(s) 29, 57, 69, 292, 619, 867
LweI GCATC 2 cut(s) 11, 69
MaeI CTAG 1 cut(s) 764
MaeII ACGT 2 cut(s) 639, 813
MaeIII GTNAC 2 cut(s) 596, 614
MalI GATC 3 cut(s) 194, 432, 589
MboI GATC 3 cut(s) 192, 430, 587
MboII GAAGA 3 cut(s) 838, 841, 846
MhlI GDGCHC 3 cut(s) 92, 808, 903
MluCI AATT 3 cut(s) 52, 837, 848
MlyI GAGTC 6 cut(s) 141, 442, 533, 604, 611, 622
MmeI TCCRAC 2 cut(s) 450, 768
MnlI CCTC 9 cut(s) 131, 167, 208, 381, 444, 619, 685, 732, 835
MroNI GCCGGC 3 cut(s) 167, 745, 869
MseI TTAA 3 cut(s) 84, 444, 582
MspR9I CCNGG 3 cut(s) 92, 271, 562
MvnI CGCG 3 cut(s) 141, 422, 881
MwoI GCNNNNNNNGC 6 cut(s) 54, 73, 302, 328, 367, 742
NaeI GCCGGC 3 cut(s) 169, 747, 871
NciI CCSGG 3 cut(s) 92, 271, 562
NdeII GATC 3 cut(s) 192, 430, 587
NgoMIV GCCGGC 3 cut(s) 167, 745, 869
NheI GCTAGC 1 cut(s) 763
NlaIII CATG 5 cut(s) 154, 222, 365, 398, 673
NlaIV GGNNCC 6 cut(s) 172, 455, 502, 744, 792, 806
NmeAIII GCCGAG 4 cut(s) 395, 521, 576, 739
NmuCI GTSAC 2 cut(s) 596, 614
NspI RCATGY 1 cut(s) 222
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 150
PceI AGGCCT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 548, 645
PdiI GCCGGC 3 cut(s) 169, 747, 871
PfeI GAWTC 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 1 cut(s) 456
Ple19I CGATCG 1 cut(s) 590
PleI GAGTC 6 cut(s) 141, 442, 533, 603, 610, 621
PpsI GAGTC 6 cut(s) 141, 442, 533, 603, 610, 621
PspFI CCCAGC 1 cut(s) 895
PspN4I GGNNCC 6 cut(s) 172, 455, 502, 744, 792, 806
PspOMI GGGCCC 1 cut(s) 804
PspPI GGNCC 6 cut(s) 170, 267, 365, 468, 804, 805
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstI CTGCAG 2 cut(s) 47, 78
PstNI CAGNNNCTG 1 cut(s) 533
PvuI CGATCG 1 cut(s) 590
RsaI GTAC 3 cut(s) 455, 559, 566
RsaNI GTAC 3 cut(s) 454, 558, 565
Rsr2I CGGWCCG 1 cut(s) 468
RsrII CGGWCCG 1 cut(s) 468
SaqAI TTAA 3 cut(s) 84, 444, 582
Sau3AI GATC 3 cut(s) 192, 430, 587
Sau96I GGNCC 6 cut(s) 170, 267, 365, 468, 804, 805
SchI GAGTC 6 cut(s) 141, 442, 533, 604, 611, 622
ScrFI CCNGG 3 cut(s) 92, 271, 562
SduI GDGCHC 3 cut(s) 92, 808, 903
SfaNI GCATC 2 cut(s) 11, 69
SfcI CTRYAG 2 cut(s) 43, 74
SinI GGWCC 2 cut(s) 267, 468
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 222
Sse9I AATT 3 cut(s) 52, 837, 848
SseBI AGGCCT 1 cut(s) 380
SspMI CTAG 1 cut(s) 764
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 90, 269, 560
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 115, 569
TaiI ACGT 2 cut(s) 642, 816
TaqI TCGA 2 cut(s) 590, 903
TaqII GACCGA 2 cut(s) 189, 241
TasI AATT 3 cut(s) 52, 837, 848
TauI GCSGC 6 cut(s) 141, 296, 350, 353, 422, 796
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 3 cut(s) 84, 444, 582
Tru9I TTAA 3 cut(s) 84, 444, 582
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 2 cut(s) 596, 614
TseI GCWGC 6 cut(s) 42, 45, 57, 280, 632, 855
Tsp45I GTSAC 2 cut(s) 596, 614
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 2 cut(s) 241, 460
TspRI CASTG 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 267, 468
XapI RAATTY 1 cut(s) 848
XceI RCATGY 1 cut(s) 222
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.