RchiOBHm_Chr2g0162621

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014406
Physical Location & Seq
Reverse (-)
78296027 .. 78297712
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53086

Sequence Viewer

Length: 903 bp
ATGCGGATGAGCTGTAATGGATGCCGAATACTACGCAAGGGGTGCAGTGAGAATTGTAGCATCAGACCCTGTTTGCAGTGGATAAAGAGCCCCGAATCCCAAGCCAACGCCACCGTTTTCCTCGCCAAGTTTTACGGCCGCGCCGGGCTCATGAACCTCATCAACGCCGGACCCGAACACCTCCGACCTGCGATTTTCCGGTCACTGCTCTACGAGGCATGCGGGAGGATTGTGAACCCGATTTACGGGTCGGTCGGGTTGTTGTGGTCCGGGTCATGGCAGCTATGCCAAGCCGCCGTGGAGAACGTGCTGAAGGGCCAGCCGATCACGCCGATCACCTCTGAGGCCGCGGCCAGCGGGCATGGCCCACCTCTCAAGGCGTACGACATTCGACACGTGTCCAAGGACGAGAACTCCGCCGCGTCCAATGACCCCCAGAAGGCAAAGACGCGCTACCGGTTCAAGAGGTCCGTCGGGAAGCCCAAGCAGCCCAACAAGACCGGGTCGGGATCCGGAGTTGAGGACGCGGCGGCTCGCTTTGAAGCCAAGCCGAACTGGACCAGCGACGAGTTCAACCGGTCGACGAGTCACGAGTCGTCGCTGAGTCACCAGTCCGAGGCTGCCAACGTGGACGGCGACAGCAAGGAAACCGAGAGCATGGTTTCCTCGGAGACGGCCGAGGCTTCGCTGTTGTTCCGAGCCGAGCCGGAGTCATCGGCTCACAAGCGAACCGCGCCGGTTCAGGAAAACGAGTTAGGTCTGGAGCTGACTCTCGGATTCGAATCGTCATCACGTGCTCCCCACGTGGTTCCGGTCAAGAAGAGAAGGGTAGACGCCTCAGGATTCTGCGGTGGGTCGTCGTCGGGCGACGGCGCGTGTAAAATGGAGCTGGGCCTTGCTTGA

Protein Analysis

300

Amino Acids

32.29

Weight (kDa)

8.69

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 4 - 102 1.6e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02550
fragaria_vesca FvH4_6g45150
malus_domestica MD09G1088700.v1.1 MD17G1078600.v1.1
prunus_persica Prupe.3G236200_v2.0.a1
pyrus_communis pycom09g01450 pycom17g07790
rosa_chinensis RchiOBHm_Chr2g0162621
rosa_laevigata RLG00000021353
rosa_multiflora Rmu_ssc0000364.1_g000001
rosa_roxburghii Rroxscaffold_2G00087820
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564500 Rh2BG577400 Rh2CG547100 Rh2DG587000
rosa_wichuraiana Rw2G046700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 2 cut(s) 581, 831
AccII CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
AccIII TCCGGA 1 cut(s) 512
AclWI GGATC 2 cut(s) 504, 517
AcoI YGGCCR 3 cut(s) 136, 351, 675
AcuI CTGAAG 1 cut(s) 332
AcvI CACGTG 3 cut(s) 397, 794, 805
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 4 cut(s) 245, 276, 439, 616
AflIII ACRYGT 2 cut(s) 394, 396
AgeI ACCGGT 2 cut(s) 456, 576
AgsI TTSAA 3 cut(s) 463, 542, 574
AluBI AGCT 4 cut(s) 12, 283, 766, 889
AluI AGCT 4 cut(s) 12, 283, 766, 889
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 2 cut(s) 504, 517
AlwNI CAGNNNCTG 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 512
AoxI GGCC 7 cut(s) 136, 316, 345, 351, 364, 675, 892
ApeKI GCWGC 3 cut(s) 280, 487, 620
AsiGI ACCGGT 2 cut(s) 456, 576
AspLEI GCGC 4 cut(s) 143, 453, 736, 875
AspS9I GGNCC 7 cut(s) 170, 267, 316, 365, 468, 558, 892
AsuC2I CCSGG 3 cut(s) 145, 271, 502
AsuHPI GGTGA 2 cut(s) 328, 599
AsuII TTCGAA 1 cut(s) 780
AvaII GGWCC 4 cut(s) 170, 267, 468, 558
AxyI CCTNAGG 1 cut(s) 838
BamHI GGATCC 1 cut(s) 509
BanII GRGCYC 2 cut(s) 92, 150
BauI CACGAG 1 cut(s) 590
BbrPI CACGTG 3 cut(s) 397, 794, 805
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 3 cut(s) 292, 499, 607
BceAI ACGGC 5 cut(s) 151, 281, 649, 690, 886
BcnI CCSGG 3 cut(s) 145, 271, 502
BcoDI GTCTC 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 196
Bme1390I CCNGG 3 cut(s) 145, 271, 502
Bme18I GGWCC 4 cut(s) 170, 267, 468, 558
BmgT120I GGNCC 7 cut(s) 170, 267, 316, 365, 468, 558, 892
BmiI GGNNCC 3 cut(s) 172, 511, 810
BmrFI CCNGG 3 cut(s) 145, 271, 502
BmsI GCATC 2 cut(s) 11, 69
BoxI GACNNNNGTC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 782
Bpu14I TTCGAA 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 3 cut(s) 145, 271, 502
BsaAI YACGTR 3 cut(s) 397, 794, 805
BsaBI GATNNNNATC 1 cut(s) 781
BsaHI GRCGYC 1 cut(s) 834
BsaJI CCNNGG 6 cut(s) 297, 348, 402, 615, 666, 678
BsaWI WCCGGW 5 cut(s) 198, 456, 512, 576, 811
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 276, 439, 616
Bse118I RCCGGY 3 cut(s) 456, 576, 736
Bse1I ACTGG 2 cut(s) 560, 610
Bse21I CCTNAGG 1 cut(s) 838
Bse8I GATNNNNATC 1 cut(s) 781
BseAI TCCGGA 1 cut(s) 512
BseDI CCNNGG 6 cut(s) 297, 348, 402, 615, 666, 678
BseGI GGATG 2 cut(s) 12, 26
BseJI GATNNNNATC 1 cut(s) 781
BseLI CCNNNNNNNGG 4 cut(s) 245, 276, 439, 616
BseMII CTCAG 3 cut(s) 333, 593, 852
BseNI ACTGG 2 cut(s) 560, 610
BseX3I CGGCCG 2 cut(s) 136, 675
BseXI GCAGC 3 cut(s) 292, 499, 607
BseYI CCCAGC 1 cut(s) 889
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
Bsh1285I CGRYCG 4 cut(s) 139, 255, 581, 678
BshFI GGCC 7 cut(s) 138, 318, 347, 353, 366, 677, 894
BshTI ACCGGT 2 cut(s) 456, 576
BsiEI CGRYCG 4 cut(s) 139, 255, 581, 678
BsiHKAI GWGCWC 1 cut(s) 799
BsiWI CGTACG 1 cut(s) 381
BslI CCNNNNNNNGG 4 cut(s) 245, 276, 439, 616
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsnI GGCC 7 cut(s) 138, 318, 347, 353, 366, 677, 894
Bsp119I TTCGAA 1 cut(s) 780
Bsp1286I GDGCHC 3 cut(s) 92, 150, 799
Bsp13I TCCGGA 1 cut(s) 512
Bsp143I GATC 3 cut(s) 324, 333, 509
BspANI GGCC 7 cut(s) 138, 318, 347, 353, 366, 677, 894
BspCNI CTCAG 3 cut(s) 334, 594, 851
BspEI TCCGGA 1 cut(s) 512
BspFNI CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 3 cut(s) 172, 511, 810
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 2 cut(s) 504, 517
BspT104I TTCGAA 1 cut(s) 780
BsrFI RCCGGY 3 cut(s) 456, 576, 736
BsrI ACTGG 2 cut(s) 560, 610
BssAI RCCGGY 3 cut(s) 456, 576, 736
BssECI CCNNGG 6 cut(s) 297, 348, 402, 615, 666, 678
BssMI GATC 3 cut(s) 324, 333, 509
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 590
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 1 cut(s) 815
BstACI GRCGYC 1 cut(s) 834
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBAI YACGTR 3 cut(s) 397, 794, 805
BstBI TTCGAA 1 cut(s) 780
BstC8I GCNNGC 5 cut(s) 220, 320, 355, 359, 535
BstDEI CTNAG 3 cut(s) 342, 602, 838
BstDSI CCRYGG 2 cut(s) 297, 348
BstF5I GGATG 2 cut(s) 12, 26
BstFNI CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
BstHHI GCGC 4 cut(s) 143, 453, 736, 875
BstKTI GATC 3 cut(s) 327, 336, 512
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 3 cut(s) 324, 333, 509
BstMCI CGRYCG 4 cut(s) 139, 255, 581, 678
BstMWI GCNNNNNNNGC 5 cut(s) 42, 328, 363, 487, 733
BstNSI RCATGY 1 cut(s) 222
BstPAI GACNNNNGTC 1 cut(s) 397
BstSCI CCNGG 3 cut(s) 143, 269, 500
BstUI CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
BstV1I GCAGC 3 cut(s) 292, 499, 607
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BstZI CGGCCG 2 cut(s) 136, 675
Bsu36I CCTNAGG 1 cut(s) 838
BsuRI GGCC 7 cut(s) 138, 318, 347, 353, 366, 677, 894
BtgI CCRYGG 2 cut(s) 297, 348
BtsCI GGATG 2 cut(s) 12, 26
BtsI GCAGTG 3 cut(s) 52, 83, 203
BtsIMutI CAGTG 3 cut(s) 52, 83, 203
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 5 cut(s) 220, 320, 355, 359, 535
CaiI CAGNNNCTG 1 cut(s) 69
CciI TCATGA 1 cut(s) 150
CfoI GCGC 4 cut(s) 143, 453, 736, 875
Cfr10I RCCGGY 3 cut(s) 456, 576, 736
Cfr13I GGNCC 7 cut(s) 170, 267, 316, 365, 468, 558, 892
Cfr42I CCGCGG 1 cut(s) 351
CseI GACGC 4 cut(s) 411, 457, 533, 842
Csp6I GTAC 1 cut(s) 382
CspAI ACCGGT 2 cut(s) 456, 576
CviAII CATG 5 cut(s) 151, 219, 276, 362, 658
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 3 cut(s) 342, 602, 838
DpnI GATC 3 cut(s) 326, 335, 511
DpnII GATC 3 cut(s) 324, 333, 509
EaeI YGGCCR 3 cut(s) 136, 351, 675
EagI CGGCCG 2 cut(s) 136, 675
Eam1104I CTCTTC 1 cut(s) 815
EarI CTCTTC 1 cut(s) 815
EciI GGCGGA 1 cut(s) 406
EclXI CGGCCG 2 cut(s) 136, 675
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 2 cut(s) 92, 150
Eco47I GGWCC 4 cut(s) 170, 267, 468, 558
Eco52I CGGCCG 2 cut(s) 136, 675
Eco57I CTGAAG 1 cut(s) 332
Eco72I CACGTG 3 cut(s) 397, 794, 805
Eco81I CCTNAGG 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 92, 150
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 5 cut(s) 154, 222, 279, 365, 661
FaiI YATR 6 cut(s) 152, 220, 277, 286, 363, 659
FatI CATG 5 cut(s) 150, 218, 275, 361, 657
FauI CCCGC 2 cut(s) 215, 350
FblI GTMKAC 2 cut(s) 581, 831
FokI GGATG 2 cut(s) 19, 33
FriOI GRGCYC 2 cut(s) 92, 150
GlaI GCGC 4 cut(s) 142, 452, 735, 874
GsaI CCCAGC 1 cut(s) 893
GsuI CTGGAG 1 cut(s) 782
HaeIII GGCC 7 cut(s) 138, 318, 347, 353, 366, 677, 894
HgaI GACGC 4 cut(s) 411, 457, 533, 842
HhaI GCGC 4 cut(s) 143, 453, 736, 875
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 5 cut(s) 154, 222, 279, 365, 661
Hin6I GCGC 4 cut(s) 141, 451, 734, 873
HinP1I GCGC 4 cut(s) 141, 451, 734, 873
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 9 cut(s) 95, 586, 593, 604, 710, 769, 777, 782, 843
HphI GGTGA 2 cut(s) 328, 599
Hpy166II GTNNAC 4 cut(s) 235, 582, 631, 832
Hpy188I TCNGA 7 cut(s) 65, 185, 343, 616, 670, 698, 776
Hpy8I GTNNAC 4 cut(s) 235, 582, 631, 832
Hpy99I CGWCG 7 cut(s) 476, 569, 586, 601, 862, 865, 872
HpyAV CCTTC 3 cut(s) 307, 433, 819
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 5 cut(s) 306, 396, 627, 793, 804
HpyCH4V TGCA 2 cut(s) 45, 76
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 328, 363, 487, 733
HpyF3I CTNAG 3 cut(s) 342, 602, 838
HpySE526I ACGT 5 cut(s) 306, 396, 627, 793, 804
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 5 cut(s) 154, 222, 279, 365, 661
HspAI GCGC 4 cut(s) 141, 451, 734, 873
Kpn2I TCCGGA 1 cut(s) 512
KspI CCGCGG 1 cut(s) 351
Kzo9I GATC 3 cut(s) 324, 333, 509
LmnI GCTCC 3 cut(s) 763, 802, 886
Lsp1109I GCAGC 3 cut(s) 292, 499, 607
LweI GCATC 2 cut(s) 11, 69
MaeII ACGT 5 cut(s) 306, 396, 627, 793, 804
MaeIII GTNAC 3 cut(s) 201, 587, 605
MalI GATC 3 cut(s) 326, 335, 511
MboI GATC 3 cut(s) 324, 333, 509
MboII GAAGA 1 cut(s) 832
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 3 cut(s) 92, 150, 799
MluCI AATT 1 cut(s) 52
MlyI GAGTC 5 cut(s) 595, 602, 613, 719, 763
MmeI TCCRAC 1 cut(s) 208
MroI TCCGGA 1 cut(s) 512
MspA1I CMGCKG 2 cut(s) 350, 357
MspR9I CCNGG 3 cut(s) 145, 271, 502
MvnI CGCG 7 cut(s) 141, 350, 422, 451, 527, 734, 875
MwoI GCNNNNNNNGC 5 cut(s) 42, 328, 363, 487, 733
NciI CCSGG 3 cut(s) 145, 271, 502
NdeII GATC 3 cut(s) 324, 333, 509
NlaIII CATG 5 cut(s) 154, 222, 279, 365, 661
NlaIV GGNNCC 3 cut(s) 172, 511, 810
NmeAIII GCCGAG 2 cut(s) 703, 727
NmuCI GTSAC 3 cut(s) 201, 587, 605
NspI RCATGY 1 cut(s) 222
NspV TTCGAA 1 cut(s) 780
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 150
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 633
PfeI GAWTC 4 cut(s) 95, 777, 782, 843
Pfl23II CGTACG 1 cut(s) 381
PflFI GACNNNGTC 1 cut(s) 502
PinAI ACCGGT 2 cut(s) 456, 576
PleI GAGTC 5 cut(s) 594, 601, 612, 718, 763
PmaCI CACGTG 3 cut(s) 397, 794, 805
PmlI CACGTG 3 cut(s) 397, 794, 805
PpsI GAGTC 5 cut(s) 594, 601, 612, 718, 763
Ppu21I YACGTR 3 cut(s) 397, 794, 805
PshAI GACNNNNGTC 1 cut(s) 397
PspCI CACGTG 3 cut(s) 397, 794, 805
PspFI CCCAGC 1 cut(s) 889
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 172, 511, 810
PspPI GGNCC 7 cut(s) 170, 267, 316, 365, 468, 558, 892
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstNI CAGNNNCTG 1 cut(s) 69
PsuI RGATCY 1 cut(s) 509
PsyI GACNNNGTC 1 cut(s) 502
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SacII CCGCGG 1 cut(s) 351
SalI GTCGAC 1 cut(s) 580
Sau3AI GATC 3 cut(s) 324, 333, 509
Sau96I GGNCC 7 cut(s) 170, 267, 316, 365, 468, 558, 892
SchI GAGTC 5 cut(s) 595, 602, 613, 719, 763
ScrFI CCNGG 3 cut(s) 145, 271, 502
SduI GDGCHC 3 cut(s) 92, 150, 799
SfaNI GCATC 2 cut(s) 11, 69
Sfr303I CCGCGG 1 cut(s) 351
SfuI TTCGAA 1 cut(s) 780
SgrBI CCGCGG 1 cut(s) 351
SinI GGWCC 4 cut(s) 170, 267, 468, 558
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 222
Sse9I AATT 1 cut(s) 52
StyD4I CCNGG 3 cut(s) 143, 269, 500
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 5 cut(s) 309, 399, 630, 796, 807
TaqI TCGA 3 cut(s) 391, 581, 780
TaqII GACCGA 1 cut(s) 241
TasI AATT 1 cut(s) 52
TauI GCSGC 7 cut(s) 141, 296, 350, 353, 422, 530, 533
TfiI GAWTC 4 cut(s) 95, 777, 782, 843
TscAI CASTG 3 cut(s) 52, 83, 210
TseFI GTSAC 3 cut(s) 201, 587, 605
TseI GCWGC 3 cut(s) 280, 487, 620
Tsp45I GTSAC 3 cut(s) 201, 587, 605
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 3 cut(s) 52, 83, 210
Tth111I GACNNNGTC 1 cut(s) 502
VpaK11BI GGWCC 4 cut(s) 170, 267, 468, 558
XceI RCATGY 1 cut(s) 222
XmiI GTMKAC 2 cut(s) 581, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.