MD17G1078600.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014406
Physical Location & Seq
Forward (+)
6506304 .. 6507665
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1078600.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCGGATAAGCTGTAATGGATGCCGAATACTCCGCAAGGGCTGCAGCGAGAATTGCAGCATCAGGCCTTGCTTGCAGTGGATTAAGAGCCCGGAGTCCCAAGCAAACGCCACCGTCTTCCTCGCCAAGTTCTACGGCCGCGCCGGACTCATGAACCTCATCAACGCCGGCCCCGAACATCTCCGCCCTGCGATCTTTCGGTCTTTGCTATACGAGGCATGCGGTCGGATTGTGAACCCGATTTACGGGTCGGTCGGGTTGTTGTGGTCCGGGAGCTGGCAGCTCTGCCAAGTCGCCGTTGAAGCCGTGCTGAAAGGCCAGCCGATTACGCCGATTACTTCCGAAGCGGCGGCGAATGGGCATGGTCCGCCTCTCAAGGCCTACGACATTCGCCACGTGTCCAAGGACGAGAACTCGGCCGCGTCTAACGATCCTCAGAAGGTTAAGACCCGGTACCGCTTCAAGCGGTCCGCTGTCAAGGCAAAGACCAACAAGGTCGGTTCCGGCTCGGCCTCTGGCTGTGGCTCGGGAGCTGATGAATCGGCCAAGCCGAGTTGGACTGGCTGCTCTGCGGACAAGTTCAACCGGTCGATGAGTCACGAGTCATCGCTGAGTCACCAGTCCGAGGCGGCTGCCAACGTGGACGGTGATAGCAAGGAATCGGAGAGCATGATTTCGTCCGAGACGGCTGAGGCTGAGCTCTTTTTCCGAGCCGAGCCCGAGTCGGCTCGCAAGCGGACGGAGTCTGTTCAGGATGGGGAGCTAGCTTTGGAGCTGACGCTTGGGTTGCAGCCGCAATCACGTGCCAACCACGTCGTTCCGGTGAAGAAGAGAAGAATTGAGGCTGGATTCGGCGCCGTATCACCGGCTGACGACGGCGCGTGTAAAATGGAGCTGGGGCTGGATTACGTGGCTTGA

Protein Analysis

306

Amino Acids

32.76

Weight (kDa)

8.63

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 4 - 103 1.1e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02550
fragaria_vesca FvH4_6g45150
malus_domestica MD09G1088700.v1.1 MD17G1078600.v1.1
prunus_persica Prupe.3G236200_v2.0.a1
pyrus_communis pycom09g01450 pycom17g07790
rosa_chinensis RchiOBHm_Chr2g0162621
rosa_laevigata RLG00000021353
rosa_multiflora Rmu_ssc0000364.1_g000001
rosa_roxburghii Rroxscaffold_2G00087820
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564500 Rh2BG577400 Rh2CG547100 Rh2DG587000
rosa_wichuraiana Rw2G046700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 2 cut(s) 453, 854
AccII CGCG 3 cut(s) 141, 422, 881
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 3 cut(s) 136, 417, 543
AcvI CACGTG 2 cut(s) 397, 803
AcyI GRCGYC 1 cut(s) 855
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 3 cut(s) 245, 276, 625
AflIII ACRYGT 1 cut(s) 396
AgeI ACCGGT 1 cut(s) 585
AgsI TTSAA 3 cut(s) 302, 463, 583
AjiI CACGTC 1 cut(s) 814
AluBI AGCT 9 cut(s) 12, 276, 283, 533, 700, 763, 767, 775, 895
AluI AGCT 9 cut(s) 12, 276, 283, 533, 700, 763, 767, 775, 895
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 1 cut(s) 425
Ama87I CYCGRG 2 cut(s) 526, 719
AoxI GGCC 8 cut(s) 65, 136, 169, 316, 378, 417, 510, 543
ApeKI GCWGC 7 cut(s) 42, 45, 57, 280, 564, 632, 790
AsiGI ACCGGT 1 cut(s) 585
Asp718I GGTACC 1 cut(s) 453
AspLEI GCGC 3 cut(s) 143, 857, 881
AspS9I GGNCC 4 cut(s) 170, 267, 365, 468
AsuC2I CCSGG 3 cut(s) 92, 271, 451
AsuHPI GGTGA 4 cut(s) 608, 659, 835, 855
AsuNHI GCTAGC 1 cut(s) 763
AvaI CYCGRG 2 cut(s) 526, 719
AvaII GGWCC 3 cut(s) 267, 365, 468
BanI GGYRCC 2 cut(s) 453, 854
BanII GRGCYC 3 cut(s) 92, 702, 720
BauI CACGAG 1 cut(s) 599
BbrPI CACGTG 2 cut(s) 397, 803
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 702
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 7 cut(s) 29, 57, 69, 292, 551, 619, 802
BccI CCATC 1 cut(s) 749
BceAI ACGGC 6 cut(s) 151, 281, 290, 702, 842, 892
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BcnI CCSGG 3 cut(s) 92, 271, 451
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 43
BfoI RGCGCY 1 cut(s) 858
BlpI GCTNAGC 1 cut(s) 696
Bme1390I CCNGG 3 cut(s) 92, 271, 451
Bme18I GGWCC 3 cut(s) 267, 365, 468
BmeT110I CYCGRG 2 cut(s) 526, 719
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 4 cut(s) 170, 267, 365, 468
BmiI GGNNCC 4 cut(s) 172, 455, 502, 856
BmrFI CCNGG 3 cut(s) 92, 271, 451
BmsI GCATC 2 cut(s) 11, 69
BmtI GCTAGC 1 cut(s) 767
BpiI GAAGAC 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 3 cut(s) 92, 271, 451
BsaAI YACGTR 3 cut(s) 397, 803, 910
BsaHI GRCGYC 1 cut(s) 855
BsaJI CCNNGG 2 cut(s) 402, 624
BsaWI WCCGGW 2 cut(s) 585, 820
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 276, 625
Bse118I RCCGGY 3 cut(s) 167, 585, 865
Bse1I ACTGG 2 cut(s) 565, 619
BseDI CCNNGG 2 cut(s) 402, 624
BseGI GGATG 2 cut(s) 26, 760
BseLI CCNNNNNNNGG 3 cut(s) 245, 276, 625
BseMII CTCAG 4 cut(s) 449, 602, 681, 687
BseNI ACTGG 2 cut(s) 565, 619
BseX3I CGGCCG 2 cut(s) 136, 417
BseXI GCAGC 7 cut(s) 29, 57, 69, 292, 551, 619, 802
BseYI CCCAGC 1 cut(s) 895
Bsh1236I CGCG 3 cut(s) 141, 422, 881
Bsh1285I CGRYCG 5 cut(s) 139, 226, 255, 420, 590
BshFI GGCC 8 cut(s) 67, 138, 171, 318, 380, 419, 512, 545
BshNI GGYRCC 2 cut(s) 453, 854
BshTI ACCGGT 1 cut(s) 585
BsiEI CGRYCG 5 cut(s) 139, 226, 255, 420, 590
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 2 cut(s) 526, 719
BsiSI CCGG 9 cut(s) 92, 144, 168, 270, 451, 504, 586, 821, 866
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 245, 276, 625
BsmAI GTCTC 1 cut(s) 677
BsmBI CGTCTC 1 cut(s) 677
BsmFI GGGAC 1 cut(s) 82
BsnI GGCC 8 cut(s) 67, 138, 171, 318, 380, 419, 512, 545
BsoBI CYCGRG 2 cut(s) 526, 719
Bsp1286I GDGCHC 3 cut(s) 92, 702, 720
Bsp143I GATC 2 cut(s) 192, 430
Bsp1720I GCTNAGC 1 cut(s) 696
BspANI GGCC 8 cut(s) 67, 138, 171, 318, 380, 419, 512, 545
BspCNI CTCAG 4 cut(s) 448, 603, 682, 688
BspFNI CGCG 3 cut(s) 141, 422, 881
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 4 cut(s) 172, 455, 502, 856
BspMAI CTGCAG 1 cut(s) 47
BspOI GCTAGC 1 cut(s) 767
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 2 cut(s) 453, 854
BsrFI RCCGGY 3 cut(s) 167, 585, 865
BsrI ACTGG 2 cut(s) 565, 619
BssAI RCCGGY 3 cut(s) 167, 585, 865
BssECI CCNNGG 2 cut(s) 402, 624
BssMI GATC 2 cut(s) 192, 430
BssNI GRCGYC 1 cut(s) 855
BssSI CACGAG 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 115, 647
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 855
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBAI YACGTR 3 cut(s) 397, 803, 910
BstC8I GCNNGC 8 cut(s) 74, 169, 220, 278, 320, 730, 734, 765
BstDEI CTNAG 4 cut(s) 435, 611, 690, 696
BstF5I GGATG 2 cut(s) 26, 760
BstFNI CGCG 3 cut(s) 141, 422, 881
BstH2I RGCGCY 1 cut(s) 858
BstHHI GCGC 3 cut(s) 143, 857, 881
BstKTI GATC 2 cut(s) 195, 433
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 2 cut(s) 192, 430
BstMCI CGRYCG 5 cut(s) 139, 226, 255, 420, 590
BstMWI GCNNNNNNNGC 8 cut(s) 42, 54, 73, 302, 328, 367, 479, 787
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 3 cut(s) 90, 269, 449
BstSFI CTRYAG 1 cut(s) 43
BstUI CGCG 3 cut(s) 141, 422, 881
BstV1I GCAGC 7 cut(s) 29, 57, 69, 292, 551, 619, 802
BstV2I GAAGAC 1 cut(s) 109
BstZI CGGCCG 2 cut(s) 136, 417
BsuRI GGCC 8 cut(s) 67, 138, 171, 318, 380, 419, 512, 545
BtgZI GCGATG 1 cut(s) 591
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 2 cut(s) 26, 760
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 8 cut(s) 74, 169, 220, 278, 320, 730, 734, 765
CciI TCATGA 1 cut(s) 150
CfoI GCGC 3 cut(s) 143, 857, 881
Cfr10I RCCGGY 3 cut(s) 167, 585, 865
Cfr13I GGNCC 4 cut(s) 170, 267, 365, 468
CpoI CGGWCCG 1 cut(s) 468
CseI GACGC 2 cut(s) 411, 787
Csp6I GTAC 1 cut(s) 454
CspAI ACCGGT 1 cut(s) 585
CspI CGGWCCG 1 cut(s) 468
CviAII CATG 4 cut(s) 151, 219, 362, 670
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 4 cut(s) 435, 611, 690, 696
DinI GGCGCC 1 cut(s) 856
DpnI GATC 2 cut(s) 194, 432
DpnII GATC 2 cut(s) 192, 430
EaeI YGGCCR 3 cut(s) 136, 417, 543
EagI CGGCCG 2 cut(s) 136, 417
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
EciI GGCGGA 2 cut(s) 173, 357
Ecl136II GAGCTC 1 cut(s) 700
EclXI CGGCCG 2 cut(s) 136, 417
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 2 cut(s) 67, 380
Eco24I GRGCYC 3 cut(s) 92, 702, 720
Eco47I GGWCC 3 cut(s) 267, 365, 468
Eco52I CGGCCG 2 cut(s) 136, 417
Eco53kI GAGCTC 1 cut(s) 700
Eco72I CACGTG 2 cut(s) 397, 803
Eco88I CYCGRG 2 cut(s) 526, 719
EcoICRI GAGCTC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 3 cut(s) 92, 702, 720
EgeI GGCGCC 1 cut(s) 856
EheI GGCGCC 1 cut(s) 856
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 4 cut(s) 154, 222, 365, 673
FaiI YATR 5 cut(s) 152, 211, 220, 363, 671
FaqI GGGAC 1 cut(s) 82
FatI CATG 4 cut(s) 150, 218, 361, 669
FokI GGATG 2 cut(s) 33, 767
FriOI GRGCYC 3 cut(s) 92, 702, 720
FspBI CTAG 1 cut(s) 764
GlaI GCGC 3 cut(s) 142, 856, 880
GsaI CCCAGC 1 cut(s) 899
HaeII RGCGCY 1 cut(s) 858
HaeIII GGCC 8 cut(s) 67, 138, 171, 318, 380, 419, 512, 545
HapII CCGG 9 cut(s) 92, 144, 168, 270, 451, 504, 586, 821, 866
HgaI GACGC 2 cut(s) 411, 787
HhaI GCGC 3 cut(s) 143, 857, 881
Hin1I GRCGYC 1 cut(s) 855
Hin1II CATG 4 cut(s) 154, 222, 365, 673
Hin6I GCGC 3 cut(s) 141, 855, 879
HinP1I GCGC 3 cut(s) 141, 855, 879
HpaII CCGG 9 cut(s) 92, 144, 168, 270, 451, 504, 586, 821, 866
HphI GGTGA 4 cut(s) 608, 659, 835, 855
Hpy166II GTNNAC 2 cut(s) 235, 643
Hpy188I TCNGA 7 cut(s) 228, 343, 438, 625, 664, 682, 710
Hpy188III TCNNGA 4 cut(s) 151, 528, 599, 752
Hpy8I GTNNAC 2 cut(s) 235, 643
Hpy99I CGWCG 2 cut(s) 818, 878
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 2 cut(s) 115, 647
HpyCH4IV ACGT 5 cut(s) 396, 639, 802, 813, 909
HpyCH4V TGCA 4 cut(s) 45, 57, 76, 790
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 54, 73, 302, 328, 367, 479, 787
HpyF3I CTNAG 4 cut(s) 435, 611, 690, 696
HpySE526I ACGT 5 cut(s) 396, 639, 802, 813, 909
Hsp92I GRCGYC 1 cut(s) 855
Hsp92II CATG 4 cut(s) 154, 222, 365, 673
HspAI GCGC 3 cut(s) 141, 855, 879
KasI GGCGCC 1 cut(s) 854
KpnI GGTACC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 192, 430
LmnI GCTCC 5 cut(s) 273, 530, 760, 772, 892
Lsp1109I GCAGC 7 cut(s) 29, 57, 69, 292, 551, 619, 802
LweI GCATC 2 cut(s) 11, 69
MaeI CTAG 1 cut(s) 764
MaeII ACGT 5 cut(s) 396, 639, 802, 813, 909
MaeIII GTNAC 2 cut(s) 596, 614
MalI GATC 2 cut(s) 194, 432
MboI GATC 2 cut(s) 192, 430
MboII GAAGA 4 cut(s) 109, 838, 841, 846
MhlI GDGCHC 3 cut(s) 92, 702, 720
MluCI AATT 2 cut(s) 52, 837
Mly113I GGCGCC 1 cut(s) 855
MlyI GAGTC 7 cut(s) 104, 141, 604, 611, 622, 731, 752
MmeI TCCRAC 2 cut(s) 206, 536
MnlI CCTC 9 cut(s) 131, 167, 208, 381, 444, 523, 619, 685, 835
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 2 cut(s) 84, 444
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 9 cut(s) 92, 144, 168, 270, 451, 504, 586, 821, 866
MspR9I CCNGG 3 cut(s) 92, 271, 451
MvnI CGCG 3 cut(s) 141, 422, 881
MwoI GCNNNNNNNGC 8 cut(s) 42, 54, 73, 302, 328, 367, 479, 787
NaeI GCCGGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 855
NciI CCSGG 3 cut(s) 92, 271, 451
NdeII GATC 2 cut(s) 192, 430
NgoMIV GCCGGC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 763
NlaIII CATG 4 cut(s) 154, 222, 365, 673
NlaIV GGNNCC 4 cut(s) 172, 455, 502, 856
NmeAIII GCCGAG 4 cut(s) 395, 488, 576, 739
NmuCI GTSAC 2 cut(s) 596, 614
NspI RCATGY 1 cut(s) 222
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 150
PceI AGGCCT 2 cut(s) 67, 380
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 548
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 3 cut(s) 539, 659, 849
PflFI GACNNNGTC 1 cut(s) 742
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 1 cut(s) 585
PleI GAGTC 7 cut(s) 103, 141, 603, 610, 621, 730, 751
PluTI GGCGCC 1 cut(s) 858
PmaCI CACGTG 2 cut(s) 397, 803
PmlI CACGTG 2 cut(s) 397, 803
PpsI GAGTC 7 cut(s) 103, 141, 603, 610, 621, 730, 751
Ppu21I YACGTR 3 cut(s) 397, 803, 910
Psp124BI GAGCTC 1 cut(s) 702
PspCI CACGTG 2 cut(s) 397, 803
PspFI CCCAGC 1 cut(s) 895
PspN4I GGNNCC 4 cut(s) 172, 455, 502, 856
PspPI GGNCC 4 cut(s) 170, 267, 365, 468
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstI CTGCAG 1 cut(s) 47
PsyI GACNNNGTC 1 cut(s) 742
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
Rsr2I CGGWCCG 1 cut(s) 468
RsrII CGGWCCG 1 cut(s) 468
SacI GAGCTC 1 cut(s) 702
SaqAI TTAA 2 cut(s) 84, 444
Sau3AI GATC 2 cut(s) 192, 430
Sau96I GGNCC 4 cut(s) 170, 267, 365, 468
SchI GAGTC 7 cut(s) 104, 141, 604, 611, 622, 731, 752
ScrFI CCNGG 3 cut(s) 92, 271, 451
SduI GDGCHC 3 cut(s) 92, 702, 720
SfaNI GCATC 2 cut(s) 11, 69
SfcI CTRYAG 1 cut(s) 43
SfoI GGCGCC 1 cut(s) 856
SinI GGWCC 3 cut(s) 267, 365, 468
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 222
Sse9I AATT 2 cut(s) 52, 837
SseBI AGGCCT 2 cut(s) 67, 380
SspDI GGCGCC 1 cut(s) 854
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 1 cut(s) 702
StuI AGGCCT 2 cut(s) 67, 380
StyD4I CCNGG 3 cut(s) 90, 269, 449
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 115, 647
TaiI ACGT 5 cut(s) 399, 642, 805, 816, 912
TaqI TCGA 1 cut(s) 590
TaqII GACCGA 2 cut(s) 189, 241
TasI AATT 2 cut(s) 52, 837
TauI GCSGC 6 cut(s) 141, 350, 353, 422, 632, 796
TfiI GAWTC 3 cut(s) 539, 659, 849
Tru1I TTAA 2 cut(s) 84, 444
Tru9I TTAA 2 cut(s) 84, 444
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 2 cut(s) 596, 614
TseI GCWGC 7 cut(s) 42, 45, 57, 280, 564, 632, 790
Tsp45I GTSAC 2 cut(s) 596, 614
TspDTI ATGAA 2 cut(s) 167, 552
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 1 cut(s) 83
Tth111I GACNNNGTC 1 cut(s) 742
VpaK11BI GGWCC 3 cut(s) 267, 365, 468
XceI RCATGY 1 cut(s) 222
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.