pycom09g01450

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014406
Physical Location & Seq
Forward (+)
1144065 .. 1145104
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01450.1

Sequence Viewer

Length: 924 bp
ATGCGGATGAGCTGTAATGGATGCCGAATACTCCGAAAGGGCTGCAGCGAAAATTGCAGCATCCGGCCTTGCCTGCAGTGGATTAAGAGCCCGGAAGCCCAAGCAAACGCCACCGTTTTCCTCGCCAAGTTCTACGGCCGCGCCGGACTCATGAACCTCGTCAACGCCGGCCCCGAACATCTCCGCCCTGCCATCTTTCGGTCTTTGCTATACGAGGCATGCGGTCGGATTGTGAACCCGATTTACGGGTCGGTCGGGTTGTTGTGGTCCGGGAGCTGGCAGCTCTGCCAAGCCGCCGTTGAAGCCGTGCTGAAAGGCCAGTCGATTACGCCGATTACTTCCGAAGCGGCGGCGGATGGGCATGGCCCGCCTCTCAAGGCCTACGACATTCGCCACGTGTCCAAGGATGAGAACTCGGCCGCGTCCAACGATCCTCAGAAGGTTAAGACTCGGTACCGGTTCAAGCGGTCCGTTGTCAAGCCGAAGACCAACAAAGTAGACTCCGGTTCTGGGTCGGGCTGTGGCTGTGGCTCAGGAGCTGATGACTCGGCCAAGCCGAGTTCTACCGGTTGCTGTGGGAACGAGTTTAACCGATCGACGAGTCACGAGTCGTCGCTAAGTCACCAGTCCGAGGCGGCTGCCAACGTGGACGGTGACAGCAAGGAATCGGAGAGCATGATTTCTTCCGAGACGGCTGAGGCTGAGCTCTTTTCCCGAGCCGAGCCCGAGCCGGCTCACAAGCGGAGGGAGCCGGTTCAGGATGGGGAGCTAGCTTTAGAGCTGACGCTTGGGTTGGAGCCGCCATCACGCGGCCACCACGTCGTTCCGGTTAAAAAGAGAAGAATTGAGGCTGAATACGGCGGATCATCGTCGGCCGACGACGGCGCGTGTAAAATGGAGCTTGGGCTCGATTACGTGGCCTGA

Protein Analysis

308

Amino Acids

32.86

Weight (kDa)

7.05

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 4 - 103 2.7e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02550
fragaria_vesca FvH4_6g45150
malus_domestica MD09G1088700.v1.1 MD17G1078600.v1.1
prunus_persica Prupe.3G236200_v2.0.a1
pyrus_communis pycom09g01450 pycom17g07790
rosa_chinensis RchiOBHm_Chr2g0162621
rosa_laevigata RLG00000021353
rosa_multiflora Rmu_ssc0000364.1_g000001
rosa_roxburghii Rroxscaffold_2G00087820
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564500 Rh2BG577400 Rh2CG547100 Rh2DG587000
rosa_wichuraiana Rw2G046700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 498
AccII CGCG 4 cut(s) 141, 422, 810, 887
AclWI GGATC 2 cut(s) 425, 871
AcoI YGGCCR 5 cut(s) 136, 417, 549, 811, 873
AcvI CACGTG 1 cut(s) 397
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 6 cut(s) 198, 245, 276, 510, 631, 809
AflIII ACRYGT 1 cut(s) 396
AgeI ACCGGT 2 cut(s) 456, 566
AgsI TTSAA 2 cut(s) 302, 463
AjiI CACGTC 1 cut(s) 820
AluBI AGCT 9 cut(s) 12, 276, 283, 539, 706, 769, 773, 781, 901
AluI AGCT 9 cut(s) 12, 276, 283, 539, 706, 769, 773, 781, 901
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 425, 871
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 2 cut(s) 714, 725
ApeKI GCWGC 5 cut(s) 42, 45, 57, 280, 638
AsiGI ACCGGT 2 cut(s) 456, 566
Asp718I GGTACC 1 cut(s) 453
AspLEI GCGC 2 cut(s) 143, 887
AspS9I GGNCC 4 cut(s) 170, 267, 365, 468
AsuC2I CCSGG 2 cut(s) 92, 271
AsuHPI GGTGA 2 cut(s) 614, 665
AsuNHI GCTAGC 1 cut(s) 769
AvaI CYCGRG 2 cut(s) 714, 725
AvaII GGWCC 2 cut(s) 267, 468
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 4 cut(s) 92, 708, 726, 909
BauI CACGAG 1 cut(s) 605
BbrPI CACGTG 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 708
BbvCI CCTCAGC 1 cut(s) 696
BbvI GCAGC 5 cut(s) 29, 57, 69, 292, 625
BccI CCATC 4 cut(s) 200, 350, 755, 811
BceAI ACGGC 6 cut(s) 151, 281, 290, 708, 874, 898
BcgI CGANNNNNNTGC 4 cut(s) 24, 58, 620, 654
BcnI CCSGG 2 cut(s) 92, 271
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 43, 74
BlpI GCTNAGC 1 cut(s) 702
Bme1390I CCNGG 2 cut(s) 92, 271
Bme18I GGWCC 2 cut(s) 267, 468
BmeT110I CYCGRG 2 cut(s) 714, 725
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 4 cut(s) 170, 267, 365, 468
BmiI GGNNCC 4 cut(s) 172, 455, 750, 798
BmrFI CCNGG 2 cut(s) 92, 271
BmsI GCATC 2 cut(s) 11, 69
BmtI GCTAGC 1 cut(s) 773
BpiI GAAGAC 1 cut(s) 491
Bpu10I CCTNAGC 2 cut(s) 532, 696
Bpu1102I GCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 92, 271
BsaAI YACGTR 2 cut(s) 397, 916
BsaJI CCNNGG 2 cut(s) 402, 630
BsaWI WCCGGW 4 cut(s) 456, 503, 566, 826
Bsc4I CCNNNNNNNGG 6 cut(s) 198, 245, 276, 510, 631, 809
Bse118I RCCGGY 5 cut(s) 167, 456, 566, 730, 751
Bse1I ACTGG 2 cut(s) 319, 625
BseDI CCNNGG 2 cut(s) 402, 630
BseGI GGATG 6 cut(s) 12, 26, 60, 361, 412, 766
BseLI CCNNNNNNNGG 6 cut(s) 198, 245, 276, 510, 631, 809
BseMII CTCAG 4 cut(s) 449, 546, 687, 693
BseNI ACTGG 2 cut(s) 319, 625
BseX3I CGGCCG 3 cut(s) 136, 417, 873
BseXI GCAGC 5 cut(s) 29, 57, 69, 292, 625
Bsh1236I CGCG 4 cut(s) 141, 422, 810, 887
Bsh1285I CGRYCG 6 cut(s) 139, 226, 255, 420, 596, 876
BshNI GGYRCC 1 cut(s) 453
BshTI ACCGGT 2 cut(s) 456, 566
BsiEI CGRYCG 6 cut(s) 139, 226, 255, 420, 596, 876
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 2 cut(s) 714, 725
BslI CCNNNNNNNGG 6 cut(s) 198, 245, 276, 510, 631, 809
BsmAI GTCTC 1 cut(s) 683
BsmBI CGTCTC 1 cut(s) 683
BsoBI CYCGRG 2 cut(s) 714, 725
Bsp1286I GDGCHC 4 cut(s) 92, 708, 726, 909
Bsp143I GATC 3 cut(s) 430, 593, 863
Bsp1720I GCTNAGC 1 cut(s) 702
BspCNI CTCAG 4 cut(s) 448, 545, 688, 694
BspFNI CGCG 4 cut(s) 141, 422, 810, 887
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 4 cut(s) 172, 455, 750, 798
BspMAI CTGCAG 2 cut(s) 47, 78
BspOI GCTAGC 1 cut(s) 773
BspPI GGATC 2 cut(s) 425, 871
BspT107I GGYRCC 1 cut(s) 453
BsrFI RCCGGY 5 cut(s) 167, 456, 566, 730, 751
BsrI ACTGG 2 cut(s) 319, 625
BssAI RCCGGY 5 cut(s) 167, 456, 566, 730, 751
BssECI CCNNGG 2 cut(s) 402, 630
BssMI GATC 3 cut(s) 430, 593, 863
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 115, 653
BstBAI YACGTR 2 cut(s) 397, 916
BstC8I GCNNGC 7 cut(s) 74, 169, 220, 278, 368, 732, 771
BstDEI CTNAG 5 cut(s) 435, 532, 617, 696, 702
BstF5I GGATG 6 cut(s) 12, 26, 60, 361, 412, 766
BstFNI CGCG 4 cut(s) 141, 422, 810, 887
BstHHI GCGC 2 cut(s) 143, 887
BstKTI GATC 3 cut(s) 433, 596, 866
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 3 cut(s) 430, 593, 863
BstMCI CGRYCG 6 cut(s) 139, 226, 255, 420, 596, 876
BstMWI GCNNNNNNNGC 5 cut(s) 54, 73, 302, 367, 748
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 90, 269
BstSFI CTRYAG 2 cut(s) 43, 74
BstUI CGCG 4 cut(s) 141, 422, 810, 887
BstV1I GCAGC 5 cut(s) 29, 57, 69, 292, 625
BstV2I GAAGAC 1 cut(s) 491
BstZI CGGCCG 3 cut(s) 136, 417, 873
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 6 cut(s) 12, 26, 60, 361, 412, 766
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 7 cut(s) 74, 169, 220, 278, 368, 732, 771
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 150
CfoI GCGC 2 cut(s) 143, 887
Cfr10I RCCGGY 5 cut(s) 167, 456, 566, 730, 751
Cfr13I GGNCC 4 cut(s) 170, 267, 365, 468
CpoI CGGWCCG 1 cut(s) 468
CseI GACGC 2 cut(s) 411, 793
Csp6I GTAC 1 cut(s) 454
CspAI ACCGGT 2 cut(s) 456, 566
CspI CGGWCCG 1 cut(s) 468
CviAII CATG 4 cut(s) 151, 219, 362, 676
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 5 cut(s) 435, 532, 617, 696, 702
DpnI GATC 3 cut(s) 432, 595, 865
DpnII GATC 3 cut(s) 430, 593, 863
EaeI YGGCCR 5 cut(s) 136, 417, 549, 811, 873
EagI CGGCCG 3 cut(s) 136, 417, 873
EciI GGCGGA 3 cut(s) 173, 368, 876
Ecl136II GAGCTC 1 cut(s) 706
EclXI CGGCCG 3 cut(s) 136, 417, 873
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 4 cut(s) 92, 708, 726, 909
Eco47I GGWCC 2 cut(s) 267, 468
Eco52I CGGCCG 3 cut(s) 136, 417, 873
Eco53kI GAGCTC 1 cut(s) 706
Eco72I CACGTG 1 cut(s) 397
Eco88I CYCGRG 2 cut(s) 714, 725
EcoICRI GAGCTC 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 4 cut(s) 92, 708, 726, 909
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 4 cut(s) 154, 222, 365, 679
FaiI YATR 5 cut(s) 152, 211, 220, 363, 677
FatI CATG 4 cut(s) 150, 218, 361, 675
FauI CCCGC 1 cut(s) 375
FblI GTMKAC 1 cut(s) 498
FokI GGATG 6 cut(s) 19, 33, 47, 368, 419, 773
FriOI GRGCYC 4 cut(s) 92, 708, 726, 909
FspBI CTAG 1 cut(s) 770
GlaI GCGC 2 cut(s) 142, 886
HgaI GACGC 2 cut(s) 411, 793
HhaI GCGC 2 cut(s) 143, 887
Hin1II CATG 4 cut(s) 154, 222, 365, 679
Hin6I GCGC 2 cut(s) 141, 885
HinP1I GCGC 2 cut(s) 141, 885
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 7 cut(s) 147, 448, 500, 545, 601, 608, 665
HphI GGTGA 2 cut(s) 614, 665
Hpy166II GTNNAC 4 cut(s) 163, 235, 499, 649
Hpy188I TCNGA 7 cut(s) 35, 228, 343, 438, 631, 670, 688
Hpy188III TCNNGA 5 cut(s) 151, 534, 605, 714, 758
Hpy8I GTNNAC 4 cut(s) 163, 235, 499, 649
Hpy99I CGWCG 6 cut(s) 601, 616, 824, 874, 881, 884
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 2 cut(s) 115, 653
HpyCH4IV ACGT 4 cut(s) 396, 645, 819, 915
HpyCH4V TGCA 3 cut(s) 45, 57, 76
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 73, 302, 367, 748
HpyF3I CTNAG 5 cut(s) 435, 532, 617, 696, 702
HpySE526I ACGT 4 cut(s) 396, 645, 819, 915
Hsp92II CATG 4 cut(s) 154, 222, 365, 679
HspAI GCGC 2 cut(s) 141, 885
KpnI GGTACC 1 cut(s) 457
KroI GCCGGC 2 cut(s) 167, 730
KroNI GCCGGC 2 cut(s) 169, 732
Kzo9I GATC 3 cut(s) 430, 593, 863
LmnI GCTCC 6 cut(s) 273, 536, 748, 766, 796, 898
Lsp1109I GCAGC 5 cut(s) 29, 57, 69, 292, 625
LweI GCATC 2 cut(s) 11, 69
MaeI CTAG 1 cut(s) 770
MaeII ACGT 4 cut(s) 396, 645, 819, 915
MaeIII GTNAC 3 cut(s) 602, 620, 653
MalI GATC 3 cut(s) 432, 595, 865
MboI GATC 3 cut(s) 430, 593, 863
MboII GAAGA 3 cut(s) 496, 675, 852
MhlI GDGCHC 4 cut(s) 92, 708, 726, 909
MluCI AATT 2 cut(s) 52, 843
MlyI GAGTC 6 cut(s) 141, 442, 494, 539, 610, 617
MmeI TCCRAC 3 cut(s) 206, 450, 774
MnlI CCTC 9 cut(s) 131, 167, 208, 381, 444, 625, 691, 738, 841
MroNI GCCGGC 2 cut(s) 167, 730
MseI TTAA 4 cut(s) 84, 444, 588, 831
MspR9I CCNGG 2 cut(s) 92, 271
MvnI CGCG 4 cut(s) 141, 422, 810, 887
MwoI GCNNNNNNNGC 5 cut(s) 54, 73, 302, 367, 748
NaeI GCCGGC 2 cut(s) 169, 732
NciI CCSGG 2 cut(s) 92, 271
NdeII GATC 3 cut(s) 430, 593, 863
NgoMIV GCCGGC 2 cut(s) 167, 730
NheI GCTAGC 1 cut(s) 769
NlaIII CATG 4 cut(s) 154, 222, 365, 679
NlaIV GGNNCC 4 cut(s) 172, 455, 750, 798
NmeAIII GCCGAG 4 cut(s) 395, 527, 582, 745
NmuCI GTSAC 3 cut(s) 602, 620, 653
NspI RCATGY 1 cut(s) 222
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 150
PceI AGGCCT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 329, 554
PdiI GCCGGC 2 cut(s) 169, 732
PfeI GAWTC 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 2 cut(s) 456, 566
Ple19I CGATCG 1 cut(s) 596
PleI GAGTC 6 cut(s) 141, 442, 494, 539, 609, 616
PmaCI CACGTG 1 cut(s) 397
PmlI CACGTG 1 cut(s) 397
PpsI GAGTC 6 cut(s) 141, 442, 494, 539, 609, 616
Ppu21I YACGTR 2 cut(s) 397, 916
Psp124BI GAGCTC 1 cut(s) 708
PspCI CACGTG 1 cut(s) 397
PspN4I GGNNCC 4 cut(s) 172, 455, 750, 798
PspPI GGNCC 4 cut(s) 170, 267, 365, 468
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstI CTGCAG 2 cut(s) 47, 78
PstNI CAGNNNCTG 1 cut(s) 539
PvuI CGATCG 1 cut(s) 596
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
Rsr2I CGGWCCG 1 cut(s) 468
RsrII CGGWCCG 1 cut(s) 468
SacI GAGCTC 1 cut(s) 708
SaqAI TTAA 4 cut(s) 84, 444, 588, 831
Sau3AI GATC 3 cut(s) 430, 593, 863
Sau96I GGNCC 4 cut(s) 170, 267, 365, 468
SchI GAGTC 6 cut(s) 141, 442, 494, 539, 610, 617
ScrFI CCNGG 2 cut(s) 92, 271
SduI GDGCHC 4 cut(s) 92, 708, 726, 909
SfaNI GCATC 2 cut(s) 11, 69
SfcI CTRYAG 2 cut(s) 43, 74
SinI GGWCC 2 cut(s) 267, 468
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 222
Sse9I AATT 2 cut(s) 52, 843
SseBI AGGCCT 1 cut(s) 380
SspMI CTAG 1 cut(s) 770
SstI GAGCTC 1 cut(s) 708
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 90, 269
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 115, 653
TaiI ACGT 4 cut(s) 399, 648, 822, 918
TaqI TCGA 3 cut(s) 323, 596, 909
TaqII GACCGA 2 cut(s) 189, 241
TasI AATT 2 cut(s) 52, 843
TauI GCSGC 8 cut(s) 141, 296, 350, 353, 422, 638, 802, 813
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 4 cut(s) 84, 444, 588, 831
Tru9I TTAA 4 cut(s) 84, 444, 588, 831
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 3 cut(s) 602, 620, 653
TseI GCWGC 5 cut(s) 42, 45, 57, 280, 638
Tsp45I GTSAC 3 cut(s) 602, 620, 653
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 267, 468
XceI RCATGY 1 cut(s) 222
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.