AT3G03690

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009545
Physical Location & Seq
Reverse (-)
911178 .. 913824
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03690.2

Sequence Viewer

Length: 1137 bp
ATGGCGATAAAGAAACTGTTGATGATCTCCTTCTCTCTTACTTCACTCCTCTTCTCTCTTCTCTACATCATCCCCACGACCAAGACTCTATTCACTTCCTCAAAAATCCCTTCTTTACCCCTCGAATCCAACCAAAACTCCAACTCTACCCTCCCCTGTTTTGCTTACCTAATCTCTGCATCCAAAGGCGACGCCGGAAAACTCAAACGCCTCCTCCGATCTCTCTACCACCGTAGAAACCATTATTTGATCCATCTAGACTTGGAGGCACCCGAGGAAGAGCATTTGGAGATGATACGGTTCGTAGCCGGAGAGCCTCTGTTTCAGCCGGAAGGTAATGTAATGATCGTTGGAAAACCGAATCTGGTGACTTACAGAGGACCAACGATGCTTGCGACGACGTTACACGCAATGGCTTTGCTCTTGAGATGTTGTCGGTGGGATTGGTTCATTAATCTTAGTGCTTCTGATTATCCTCTCGTCACACAAGACGATCTGATCTATGCATTTTCTGAGTTGCCTAGAGATCTCAACTTCATACAGCACACGAGCCGATTAGGATGGAAAATGAACAAAAGAGGGAAACCAATAATAATAGATCCAGGGCTTTATAGCCTCAACAAATCAGAGATTTGGTGGGTTAGTAATCAGCGAAGCCTCCCTACTTCTTTCAAGCTCTTCACAGGTTCTGCTTGGACATTCCTATCAAGACCGTTCGCAGAATATTGTATAATAGGTTATGACAACTTACCAAGAACACTGCTTCTCTACTACACAAACTTCGTTTCATCCCCGGAAGGCTATTTTCAGACGCTTATATGCAACTCGGATGAGTTCAAGAACACGACCGTGAACCACGACCTCCATTACATCGCGTGGGACAATCCGCCAAAGCAGCACCCCAAGATATTAGGGACAAGGGATTACAGAAAGATGGTAATGAGCAACCGACCATTCGCTAGGAAGTTCAAGAGCAATGACCCCGTTCTCAATAGGATAGACCGGGAGATTCTGAGAAGGAAACGGAAACTTGGGTCGAAACCCGATCTTGGTCCTGGTCCGGGGGCTAGAAGATTGAAGAGTTTACTAATGAGGCTTTTGTTGAGAAGAAACTTTGTCAATAGACAATGTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

378

Amino Acids

43.82

Weight (kDa)

9.88

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 54 - 190 1.9e-37 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 1 cut(s) 875
AciI CCGC 1 cut(s) 887
AclWI GGATC 2 cut(s) 244, 593
AcyI GRCGYC 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 1049, 1061
AgsI TTSAA 4 cut(s) 673, 838, 970, 1078
AjnI CCWGG 2 cut(s) 601, 1054
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AleI CACNNNNGTG 1 cut(s) 848
AluBI AGCT 1 cut(s) 676
AluI AGCT 1 cut(s) 676
AlwI GGATC 2 cut(s) 244, 593
AlwNI CAGNNNCTG 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 895
AseI ATTAAT 1 cut(s) 453
AspS9I GGNCC 3 cut(s) 380, 1052, 1058
AsuC2I CCSGG 3 cut(s) 794, 1004, 1062
AsuHPI GGTGA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 3 cut(s) 380, 1052, 1058
BanI GGYRCC 1 cut(s) 268
BarI GAAGNNNNNNTAC 2 cut(s) 646, 678
BauI CACGAG 1 cut(s) 547
BbvI GCAGC 1 cut(s) 907
BccI CCATC 3 cut(s) 261, 555, 928
BciT130I CCWGG 2 cut(s) 603, 1056
BcnI CCSGG 3 cut(s) 794, 1004, 1062
BfaI CTAG 4 cut(s) 257, 522, 960, 1068
BglII AGATCT 1 cut(s) 526
BisI GCNGC 1 cut(s) 896
BlsI GCNGC 1 cut(s) 897
Bme1390I CCNGG 5 cut(s) 603, 794, 1004, 1056, 1062
Bme18I GGWCC 3 cut(s) 380, 1052, 1058
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 3 cut(s) 380, 1052, 1058
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 5 cut(s) 603, 794, 1004, 1056, 1062
BmsI GCATC 2 cut(s) 188, 378
BpuEI CTTGAG 1 cut(s) 445
BpuMI CCSGG 3 cut(s) 794, 1004, 1062
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 273, 602, 792, 1061
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 198, 228
Bsc4I CCNNNNNNNGG 2 cut(s) 1049, 1061
Bse3DI GCAATG 2 cut(s) 417, 982
BseBI CCWGG 2 cut(s) 603, 1056
BseDI CCNNGG 4 cut(s) 273, 602, 792, 1061
BseGI GGATG 5 cut(s) 69, 179, 566, 788, 835
BseLI CCNNNNNNNGG 2 cut(s) 1049, 1061
BseMI GCAATG 2 cut(s) 417, 982
BseMII CTCAG 2 cut(s) 504, 1004
BseRI GAGGAG 2 cut(s) 38, 203
BseXI GCAGC 1 cut(s) 907
Bsh1236I CGCG 1 cut(s) 875
Bsh1285I CGRYCG 1 cut(s) 849
BshNI GGYRCC 1 cut(s) 268
BsiEI CGRYCG 1 cut(s) 849
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 6 cut(s) 195, 309, 329, 794, 1003, 1061
BslFI GGGAC 2 cut(s) 893, 928
BslI CCNNNNNNNGG 2 cut(s) 1049, 1061
BsmFI GGGAC 2 cut(s) 893, 928
BsoBI CYCGRG 1 cut(s) 272
Bsp143I GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
BspACI CCGC 1 cut(s) 887
BspCNI CTCAG 2 cut(s) 505, 1005
BspFNI CGCG 1 cut(s) 875
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 2 cut(s) 244, 593
BspQI GCTCTTC 2 cut(s) 273, 683
BspT107I GGYRCC 1 cut(s) 268
BsrDI GCAATG 2 cut(s) 417, 982
BssECI CCNNGG 4 cut(s) 273, 602, 792, 1061
BssMI GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 603, 1056
Bst4CI ACNGT 5 cut(s) 18, 233, 300, 714, 850
Bst6I CTCTTC 5 cut(s) 56, 63, 273, 683, 1073
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 3 cut(s) 458, 513, 1013
BstF5I GGATG 5 cut(s) 69, 179, 566, 788, 835
BstFNI CGCG 1 cut(s) 875
BstKTI GATC 9 cut(s) 27, 221, 252, 348, 496, 501, 529, 601, 1048
BstMBI GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
BstMCI CGRYCG 1 cut(s) 849
BstMWI GCNNNNNNNGC 1 cut(s) 895
BstNI CCWGG 2 cut(s) 603, 1056
BstSCI CCNGG 5 cut(s) 601, 792, 1002, 1054, 1060
BstUI CGCG 1 cut(s) 875
BstV1I GCAGC 1 cut(s) 907
BstX2I RGATCY 2 cut(s) 526, 598
BstYI RGATCY 2 cut(s) 526, 598
BtgZI GCGATG 1 cut(s) 856
BtsCI GGATG 5 cut(s) 69, 179, 566, 788, 835
BtsI GCAGTG 1 cut(s) 758
BtsIMutI CAGTG 1 cut(s) 758
Cac8I GCNNGC 1 cut(s) 393
CaiI CAGNNNCTG 1 cut(s) 689
Cfr13I GGNCC 3 cut(s) 380, 1052, 1058
CseI GACGC 2 cut(s) 200, 820
DdeI CTNAG 3 cut(s) 458, 513, 1013
DpnI GATC 9 cut(s) 26, 220, 251, 347, 495, 500, 528, 600, 1047
DpnII GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
Eam1104I CTCTTC 5 cut(s) 56, 63, 273, 683, 1073
EarI CTCTTC 5 cut(s) 56, 63, 273, 683, 1073
EciI GGCGGA 1 cut(s) 876
Eco47I GGWCC 3 cut(s) 380, 1052, 1058
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 601, 1054
EcoT22I ATGCAT 1 cut(s) 508
FaiI YATR 7 cut(s) 504, 539, 612, 731, 741, 818, 820
FaqI GGGAC 2 cut(s) 893, 928
Fnu4HI GCNGC 1 cut(s) 896
FokI GGATG 5 cut(s) 56, 166, 573, 775, 842
Fsp4HI GCNGC 1 cut(s) 896
FspBI CTAG 4 cut(s) 257, 522, 960, 1068
GluI GCNGC 1 cut(s) 896
HapII CCGG 6 cut(s) 195, 309, 329, 794, 1003, 1061
HgaI GACGC 2 cut(s) 200, 820
Hin1I GRCGYC 1 cut(s) 192
HinfI GANTC 4 cut(s) 85, 125, 361, 1009
HpaII CCGG 6 cut(s) 195, 309, 329, 794, 1003, 1061
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 2 cut(s) 853, 1085
Hpy188I TCNGA 8 cut(s) 218, 469, 498, 514, 628, 810, 829, 1014
Hpy188III TCNNGA 5 cut(s) 257, 424, 708, 838, 970
Hpy8I GTNNAC 2 cut(s) 853, 1085
Hpy99I CGWCG 3 cut(s) 194, 400, 403
HpyAV CCTTC 5 cut(s) 40, 120, 326, 791, 1011
HpyCH4III ACNGT 5 cut(s) 18, 233, 300, 714, 850
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 3 cut(s) 179, 506, 822
HpyF10VI GCNNNNNNNGC 1 cut(s) 895
HpyF3I CTNAG 3 cut(s) 458, 513, 1013
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 192
Kzo9I GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
LguI GCTCTTC 2 cut(s) 273, 683
Lsp1109I GCAGC 1 cut(s) 907
LweI GCATC 2 cut(s) 188, 378
MaeI CTAG 4 cut(s) 257, 522, 960, 1068
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 367, 402, 481
MalI GATC 9 cut(s) 26, 220, 251, 347, 495, 500, 528, 600, 1047
MboI GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
MboII GAAGA 7 cut(s) 43, 50, 290, 670, 1083, 1090, 1119
MflI RGATCY 2 cut(s) 526, 598
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 3 cut(s) 153, 165, 331
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 848
MspI CCGG 6 cut(s) 195, 309, 329, 794, 1003, 1061
MspR9I CCNGG 5 cut(s) 603, 794, 1004, 1056, 1062
MvaI CCWGG 2 cut(s) 603, 1056
MvnI CGCG 1 cut(s) 875
MwoI GCNNNNNNNGC 1 cut(s) 895
NciI CCSGG 3 cut(s) 794, 1004, 1062
NdeII GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
NlaIV GGNNCC 1 cut(s) 270
NmuCI GTSAC 2 cut(s) 367, 481
NsiI ATGCAT 1 cut(s) 508
OliI CACNNNNGTG 1 cut(s) 848
PciSI GCTCTTC 2 cut(s) 273, 683
PcsI WCGNNNNNNNCGW 2 cut(s) 214, 392
PfeI GAWTC 3 cut(s) 125, 361, 1009
PkrI GCNGC 1 cut(s) 897
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 601, 1054
PspGI CCWGG 2 cut(s) 601, 1054
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 3 cut(s) 380, 1052, 1058
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 526, 598
RseI CAYNNNNRTG 1 cut(s) 848
SapI GCTCTTC 2 cut(s) 273, 683
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 1 cut(s) 896
Sau3AI GATC 9 cut(s) 24, 218, 249, 345, 493, 498, 526, 598, 1045
Sau96I GGNCC 3 cut(s) 380, 1052, 1058
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 5 cut(s) 603, 794, 1004, 1056, 1062
SetI ASST 7 cut(s) 171, 337, 404, 678, 688, 739, 864
SfaNI GCATC 2 cut(s) 188, 378
SinI GGWCC 3 cut(s) 380, 1052, 1058
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SsiI CCGC 1 cut(s) 887
SspI AATATT 1 cut(s) 725
SspMI CTAG 4 cut(s) 257, 522, 960, 1068
StyD4I CCNGG 5 cut(s) 601, 792, 1002, 1054, 1060
TaaI ACNGT 5 cut(s) 18, 233, 300, 714, 850
TaiI ACGT 1 cut(s) 404
TaqI TCGA 2 cut(s) 123, 1037
TfiI GAWTC 3 cut(s) 125, 361, 1009
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 2 cut(s) 367, 481
TseI GCWGC 1 cut(s) 895
Tsp45I GTSAC 2 cut(s) 367, 481
TspDTI ATGAA 4 cut(s) 439, 526, 584, 777
TspGWI ACGGA 1 cut(s) 1039
TspRI CASTG 1 cut(s) 765
VpaK11BI GGWCC 3 cut(s) 380, 1052, 1058
VspI ATTAAT 1 cut(s) 453
XbaI TCTAGA 1 cut(s) 256
XspI CTAG 4 cut(s) 257, 522, 960, 1068
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.