MD14G1009700.v1.1

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009545
Physical Location & Seq
Forward (+)
960428 .. 964774
4347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1009700.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGCCATAAAAGTTTGTGTAATATCTTTCCTCCTAACCTCAGTTCTCTTCTCTCTTCTATACATCCCAACTAGGTTAAAAATCCCTATCCACAACTTCAAGCCAGTCATAAACTACCTCAACTTAACCACCATATCCAGCAACAGCACCACCAAGCCATATCCAGTGACATTTTCCTACCTAATCTCTGCATCCAAAGGCGATTCCATGAAGCTAAAGCGCATGCTGTATGCCCTATACCATCCTGGAAATTACTATCTAATCCATATGGATTACGCTGCACCAGAAGATGAGCACAGGGACATTGCTGACTTTGTGGCTGGAGATCCAGTTTTTGGGCAAGTTGGTAATGTTTGGGTTTTGGGAAAACCTAATTTGGTCACTTACAGAGGACCAACCATGCTTGCCACTACTCTTCATGCATTGTCCTTGCTCCTGAGGACTTGGGATTGGGATTGGTTTATCAATCTTAGTGCTTCTGATTATCCTTTAATCACTCAAGATGATCTGATACATGCTTTTTCTGATTTACCAAGAGGTCTCAATTTCATACATCACACTAGTCGCTTGGGTTGGAAGCTAAATAAGAGAGGGAAGCCAATGGTAATAGACCCAGGACTGTACAGCCTGAATAAATCAGAGTTGTGGTGGGTTATCAAACAAAGGGCTCTCCCAACTGCTTTCAATCTTTACACAGGTTCAGCTTGGACAATTTTATCAAGATCTTTTGCCGAGTATTGTGTCGTAGGGTGGGACAATCTGCCAAGGACTCTCCTCCTCTACTACACTAACTTTGTTTCATCACCGGAAGGCTACTTCCAGACAGTAATATGTAACTCCGAAGACTACAAGAACACCACCGTCAATCACGACCTTCATTACATTACATGGGATACACCTCCGAAGCAGCATCCGAGGTCTCTTGGACTGAGAGATCTCAGAAGAATGATTCTGAGCAACCGTCCATTTGCTAGAAAGTTCAAGAAAAATGCCCCGGTTCTAAACAAAATTGATCGCGACCTTCTTAAACGACATCGCGGACAATTTGCTTACGGGGGATGGTGTTCTGGGAGTAATGGGAAGACGCAAAAGGTATGCTCGAGTTTGCAGAGTGAAAACTATGGCGTTTTAAGGCCGGGACCGGGGTCTAGGAGGTTGAAATCTTTGTTAACGAAACTCCTCTCCACAAGGAACTTCAAGAAACAACAATGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

406

Amino Acids

46.51

Weight (kDa)

9.78

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 316 2.4e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 335
AccII CGCG 2 cut(s) 1017, 1038
AciI CCGC 1 cut(s) 1038
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 2 cut(s) 335, 1142
AgsI TTSAA 5 cut(s) 100, 685, 982, 1159, 1198
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 2 cut(s) 244, 613
AluBI AGCT 3 cut(s) 214, 580, 704
AluI AGCT 3 cut(s) 214, 580, 704
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 545, 924
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 1099
AoxI GGCC 2 cut(s) 3, 1133
ApeKI GCWGC 2 cut(s) 278, 907
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 392, 1139
AsuC2I CCSGG 3 cut(s) 995, 1137, 1143
AsuHPI GGTGA 1 cut(s) 795
AvaI CYCGRG 1 cut(s) 1099
AvaII GGWCC 2 cut(s) 392, 1139
AxyI CCTNAGG 1 cut(s) 437
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 670
BbsI GAAGAC 2 cut(s) 849, 1088
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 265, 919
BccI CCATC 2 cut(s) 249, 1053
BciT130I CCWGG 2 cut(s) 246, 615
BciVI GTATCC 1 cut(s) 886
BcnI CCSGG 3 cut(s) 995, 1137, 1143
BcoDI GTCTC 2 cut(s) 545, 924
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 4 cut(s) 72, 561, 972, 1149
BfuI GTATCC 1 cut(s) 886
BglII AGATCT 2 cut(s) 722, 934
BisI GCNGC 2 cut(s) 279, 908
BlsI GCNGC 2 cut(s) 280, 909
Bme1390I CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
Bme18I GGWCC 2 cut(s) 392, 1139
BmeT110I CYCGRG 1 cut(s) 1099
BmgT120I GGNCC 2 cut(s) 392, 1139
BmiI GGNNCC 1 cut(s) 1140
BmrFI CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
BmsI GCATC 2 cut(s) 200, 919
BoxI GACNNNNGTC 1 cut(s) 1144
BpiI GAAGAC 2 cut(s) 849, 1088
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 483
BpuMI CCSGG 3 cut(s) 995, 1137, 1143
BsaI GGTCTC 2 cut(s) 545, 924
BsaJI CCNNGG 5 cut(s) 613, 764, 914, 993, 1142
BsaWI WCCGGW 1 cut(s) 805
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 1142
Bse1I ACTGG 3 cut(s) 104, 164, 329
Bse21I CCTNAGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 2 cut(s) 246, 615
BseDI CCNNGG 5 cut(s) 613, 764, 914, 993, 1142
BseGI GGATG 5 cut(s) 63, 191, 241, 910, 1064
BseLI CCNNNNNNNGG 2 cut(s) 335, 1142
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 5 cut(s) 54, 428, 920, 944, 952
BseNI ACTGG 3 cut(s) 104, 164, 329
BseRI GAGGAG 3 cut(s) 764, 767, 1169
BseXI GCAGC 2 cut(s) 265, 919
BsgI GTGCAG 1 cut(s) 264
Bsh1236I CGCG 2 cut(s) 1017, 1038
BshFI GGCC 2 cut(s) 5, 1135
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 1099
BsiSI CCGG 4 cut(s) 806, 995, 1136, 1142
BslFI GGGAC 3 cut(s) 314, 767, 1152
BslI CCNNNNNNNGG 2 cut(s) 335, 1142
BsmAI GTCTC 2 cut(s) 545, 924
BsmFI GGGAC 3 cut(s) 314, 767, 1152
BsnI GGCC 2 cut(s) 5, 1135
Bso31I GGTCTC 2 cut(s) 545, 924
BsoBI CYCGRG 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 297, 670
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 5 cut(s) 325, 505, 722, 934, 1012
Bsp68I TCGCGA 1 cut(s) 1017
BspACI CCGC 1 cut(s) 1038
BspANI GGCC 2 cut(s) 5, 1135
BspCNI CTCAG 5 cut(s) 53, 429, 921, 945, 951
BspFNI CGCG 2 cut(s) 1017, 1038
BspLI GGNNCC 1 cut(s) 1140
BspPI GGATC 1 cut(s) 320
BspTNI GGTCTC 2 cut(s) 545, 924
BsrDI GCAATG 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 3 cut(s) 104, 164, 329
BssECI CCNNGG 5 cut(s) 613, 764, 914, 993, 1142
BssMI GATC 5 cut(s) 325, 505, 722, 934, 1012
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 246, 615
Bst4CI ACNGT 4 cut(s) 621, 826, 862, 962
Bst6I CTCTTC 3 cut(s) 53, 60, 420
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 2 cut(s) 224, 405
BstDEI CTNAG 6 cut(s) 40, 437, 470, 929, 938, 953
BstF5I GGATG 5 cut(s) 63, 191, 241, 910, 1064
BstFNI CGCG 2 cut(s) 1017, 1038
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 5 cut(s) 328, 508, 725, 937, 1015
BstMAI GTCTC 2 cut(s) 545, 924
BstMBI GATC 5 cut(s) 325, 505, 722, 934, 1012
BstNI CCWGG 2 cut(s) 246, 615
BstNSI RCATGY 2 cut(s) 226, 518
BstPAI GACNNNNGTC 1 cut(s) 1144
BstSCI CCNGG 5 cut(s) 244, 613, 993, 1135, 1141
BstUI CGCG 2 cut(s) 1017, 1038
BstV1I GCAGC 2 cut(s) 265, 919
BstV2I GAAGAC 2 cut(s) 849, 1088
BstX2I RGATCY 3 cut(s) 325, 722, 934
BstYI RGATCY 3 cut(s) 325, 722, 934
Bsu36I CCTNAGG 1 cut(s) 437
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 2 cut(s) 5, 1135
BtgZI GCGATG 1 cut(s) 1019
BtsCI GGATG 5 cut(s) 63, 191, 241, 910, 1064
BtsIMutI CAGTG 1 cut(s) 171
BtuMI TCGCGA 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 224, 405
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 392, 1139
CseI GACGC 1 cut(s) 1093
Csp6I GTAC 1 cut(s) 622
CviAII CATG 6 cut(s) 208, 223, 400, 419, 515, 888
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 6 cut(s) 40, 437, 470, 929, 938, 953
DpnI GATC 5 cut(s) 327, 507, 724, 936, 1014
DpnII GATC 5 cut(s) 325, 505, 722, 934, 1012
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 53, 60, 420
EarI CTCTTC 3 cut(s) 53, 60, 420
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 670
Eco31I GGTCTC 2 cut(s) 545, 924
Eco47I GGWCC 2 cut(s) 392, 1139
Eco81I CCTNAGG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 1099
EcoRII CCWGG 2 cut(s) 244, 613
EcoT14I CCWWGG 1 cut(s) 764
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 6 cut(s) 211, 226, 403, 422, 518, 891
FaqI GGGAC 3 cut(s) 314, 767, 1152
FatI CATG 6 cut(s) 207, 222, 399, 418, 514, 887
FauNDI CATATG 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 279, 908
FokI GGATG 5 cut(s) 50, 178, 228, 897, 1071
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 2 cut(s) 279, 908
FspBI CTAG 4 cut(s) 72, 561, 972, 1149
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 279, 908
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 5, 1135
HapII CCGG 4 cut(s) 806, 995, 1136, 1142
HgaI GACGC 1 cut(s) 1093
HhaI GCGC 1 cut(s) 222
Hin1II CATG 6 cut(s) 211, 226, 403, 422, 518, 891
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 1 cut(s) 1170
HindII GTYRAC 1 cut(s) 1170
HinfI GANTC 3 cut(s) 203, 769, 949
HpaI GTTAAC 1 cut(s) 1170
HpaII CCGG 4 cut(s) 806, 995, 1136, 1142
HphI GGTGA 1 cut(s) 795
Hpy166II GTNNAC 1 cut(s) 1170
Hpy188I TCNGA 9 cut(s) 481, 510, 526, 640, 841, 903, 915, 941, 954
Hpy188III TCNNGA 8 cut(s) 436, 500, 720, 820, 869, 982, 1016, 1198
Hpy8I GTNNAC 1 cut(s) 1170
HpyAV CCTTC 3 cut(s) 803, 884, 1031
HpyCH4III ACNGT 4 cut(s) 621, 826, 862, 962
HpyCH4V TGCA 5 cut(s) 191, 281, 422, 1108, 1212
HpyF3I CTNAG 6 cut(s) 40, 437, 470, 929, 938, 953
Hsp92II CATG 6 cut(s) 211, 226, 403, 422, 518, 891
HspAI GCGC 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 1170
Kzo9I GATC 5 cut(s) 325, 505, 722, 934, 1012
LmnI GCTCC 1 cut(s) 438
Lsp1109I GCAGC 2 cut(s) 265, 919
LweI GCATC 2 cut(s) 200, 919
MaeI CTAG 4 cut(s) 72, 561, 972, 1149
MaeIII GTNAC 3 cut(s) 166, 379, 833
MalI GATC 5 cut(s) 327, 507, 724, 936, 1014
MboI GATC 5 cut(s) 325, 505, 722, 934, 1012
MboII GAAGA 7 cut(s) 40, 47, 299, 407, 854, 954, 1093
MflI RGATCY 3 cut(s) 325, 722, 934
MhlI GDGCHC 2 cut(s) 297, 670
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 250, 373, 544, 711, 1008, 1043
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 1 cut(s) 554
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 424
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 77, 125, 491, 1026, 1130, 1169
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 806, 995, 1136, 1142
MspR9I CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
MvaI CCWGG 2 cut(s) 246, 615
MvnI CGCG 2 cut(s) 1017, 1038
NciI CCSGG 3 cut(s) 995, 1137, 1143
NdeI CATATG 1 cut(s) 267
NdeII GATC 5 cut(s) 325, 505, 722, 934, 1012
NlaIII CATG 6 cut(s) 211, 226, 403, 422, 518, 891
NlaIV GGNNCC 1 cut(s) 1140
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 2 cut(s) 166, 379
NruI TCGCGA 1 cut(s) 1017
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 226, 518
PaeI GCATGC 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 1099
PfeI GAWTC 2 cut(s) 203, 949
PflMI CCANNNNNTGG 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 2 cut(s) 280, 909
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
PshAI GACNNNNGTC 1 cut(s) 1144
Psp6I CCWGG 2 cut(s) 244, 613
PspGI CCWGG 2 cut(s) 244, 613
PspN4I GGNNCC 1 cut(s) 1140
PspPI GGNCC 2 cut(s) 392, 1139
PspXI VCTCGAGB 1 cut(s) 1099
PsuI RGATCY 3 cut(s) 325, 722, 934
RruI TCGCGA 1 cut(s) 1017
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
SaqAI TTAA 6 cut(s) 77, 125, 491, 1026, 1130, 1169
SatI GCNGC 2 cut(s) 279, 908
Sau3AI GATC 5 cut(s) 325, 505, 722, 934, 1012
Sau96I GGNCC 2 cut(s) 392, 1139
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
SduI GDGCHC 2 cut(s) 297, 670
SfaNI GCATC 2 cut(s) 200, 919
Sfr274I CTCGAG 1 cut(s) 1099
SinI GGWCC 2 cut(s) 392, 1139
SlaI CTCGAG 1 cut(s) 1099
SmlI CTYRAG 2 cut(s) 498, 1099
SmoI CTYRAG 2 cut(s) 498, 1099
SpeI ACTAGT 1 cut(s) 560
SphI GCATGC 1 cut(s) 226
Sse9I AATT 6 cut(s) 250, 373, 544, 711, 1008, 1043
SsiI CCGC 1 cut(s) 1038
SspMI CTAG 4 cut(s) 72, 561, 972, 1149
StyD4I CCNGG 5 cut(s) 244, 613, 993, 1135, 1141
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 4 cut(s) 621, 826, 862, 962
TaqI TCGA 1 cut(s) 1100
TasI AATT 6 cut(s) 250, 373, 544, 711, 1008, 1043
TatI WGTACW 1 cut(s) 621
TfiI GAWTC 2 cut(s) 203, 949
Tru1I TTAA 6 cut(s) 77, 125, 491, 1026, 1130, 1169
Tru9I TTAA 6 cut(s) 77, 125, 491, 1026, 1130, 1169
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 2 cut(s) 166, 379
TseI GCWGC 2 cut(s) 278, 907
Tsp45I GTSAC 2 cut(s) 166, 379
TspDTI ATGAA 5 cut(s) 224, 407, 538, 789, 866
TspRI CASTG 1 cut(s) 171
Van91I CCANNNNNTGG 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 392, 1139
XceI RCATGY 2 cut(s) 226, 518
XhoI CTCGAG 1 cut(s) 1099
XspI CTAG 4 cut(s) 72, 561, 972, 1149
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.