MD12G1012100.v1.1

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009545
Physical Location & Seq
Forward (+)
1229466 .. 1233570
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1012100.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGCCATCAAAGTTTGTGTGATATCTTTCCTCCTAACCTCAGTTCTCTTCTCTCTCCTATACATCCCAACTAGGTTAAAAATCCACATCCACAACTTCAAGCCGGTCATAAACTACTTCAACTTAACCACCATATCCAACACCAGCAACACCAAGCCATATCCAGTGACATTTGCTTACCTAATCTCTGCATCCAAGGGCGATTCCATGAAGCTAAAACGCATGCTGTACGCCCTATACCATCCTGGAAATTACTATCTAATCCACATGGATTACGGTGCACCAGAAGATGAGCACAGGGACATTGCTGACTTCGTGGCTGGAGATCCAGTTTTTGGGCAAGTTGGTAATGTTTGGATTTTGGGAAAACCTAATTTGGTCACTTATAGAGGACCAACAATGCTTGCTACTACTCTTCATGCGTTGTCTTTGCTCTTGAGGACTTGGGATTGGGATTGGTTTATCAATCTTAGTGCTTCTGATTATCCTTTAATCACTCAAGATGATATGATCCATGCGTTTTCTGATTTGCCAAGAGATCTCAATTTTATACAGCACACCAGTCGCTTGGGTTGGAAGCTGAATAAGAGAGGCAAGCCAATGATAATAGACCCAGGACTGTACAGCCTCAATAAATCAGAGTTGTGGTGGGTAATTAAACAAAGGGCTCTCCCAACTGCATTCAAGCTTTACACAGGTTCAGCGTGGACAATTTTATCAAGATCTTTTTCCGAGTACTGCATAGTAGGGTGGGACAATCTGCCAAGAACTCTCCTCCTCTACTACACTAACTTTGTCTCATCACCTGAAGGCTACTTCCAAACGGTAATATGCAACTCCGAATACTACAAGAACACCACGGTCAATCACGACCTTCACTACATTACATGGGATACACCTCCAAAGCAGCACCCGAGGTCTCTTGGACTCAGAGATTTTAGAAGAATGATTCAAAGCAACCGTCCATTTGCTCGAAAGTTCAAGAAAAACGCCCCGGTTCTAAACAAAATCGATCGTGAACTTCTTAAACGACATCGCGGAGAATTTGCTTATGGGGGGTGGTGTTCTGGGAGTAATGGGAAGACGCAAAAAGTATGCTCGAGTTTGCAGAGTGAAAACTATGGCGTTTTAAGGCCAGGACCGGGGTCTAGGAGGTTGAAATCTTTGTTAACGAAACTCCTCTCCACAAGGAACTTCAAGAAACAACAATGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

406

Amino Acids

46.83

Weight (kDa)

9.75

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 58 - 316 1.2e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 335
AccII CGCG 1 cut(s) 1038
AciI CCGC 1 cut(s) 1038
AclWI GGATC 2 cut(s) 320, 505
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1043
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 3 cut(s) 230, 623, 737
AfiI CCNNNNNNNGG 2 cut(s) 335, 1142
AgsI TTSAA 7 cut(s) 100, 121, 685, 953, 982, 1159, 1198
AjnI CCWGG 3 cut(s) 244, 613, 1135
AluBI AGCT 3 cut(s) 214, 580, 688
AluI AGCT 3 cut(s) 214, 580, 688
Alw21I GWGCWC 2 cut(s) 283, 297
Alw26I GTCTC 2 cut(s) 802, 924
Alw44I GTGCAC 1 cut(s) 279
AlwI GGATC 2 cut(s) 320, 505
Ama87I CYCGRG 2 cut(s) 913, 1099
AoxI GGCC 2 cut(s) 3, 1133
ApaLI GTGCAC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 907
ApoI RAATTY 1 cut(s) 1043
ArsI GACNNNNNNTTYG 2 cut(s) 946, 978
AspS9I GGNCC 2 cut(s) 392, 1139
AsuC2I CCSGG 2 cut(s) 995, 1143
AsuHPI GGTGA 1 cut(s) 795
AvaI CYCGRG 2 cut(s) 913, 1099
AvaII GGWCC 2 cut(s) 392, 1139
BaeGI GKGCMC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 670
BbsI GAAGAC 1 cut(s) 1088
Bbv12I GWGCWC 2 cut(s) 283, 297
BbvI GCAGC 1 cut(s) 919
BccI CCATC 2 cut(s) 14, 249
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 3 cut(s) 246, 615, 1137
BciVI GTATCC 1 cut(s) 886
BcnI CCSGG 2 cut(s) 995, 1143
BcoDI GTCTC 2 cut(s) 802, 924
BfaI CTAG 2 cut(s) 72, 1149
BfuI GTATCC 1 cut(s) 886
BglII AGATCT 2 cut(s) 538, 722
BisI GCNGC 1 cut(s) 908
BlsI GCNGC 1 cut(s) 909
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
Bme18I GGWCC 2 cut(s) 392, 1139
BmeT110I CYCGRG 2 cut(s) 913, 1099
BmgT120I GGNCC 2 cut(s) 392, 1139
BmrFI CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
BmsI GCATC 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 1144
BpiI GAAGAC 1 cut(s) 1088
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 457, 483
BpuMI CCSGG 2 cut(s) 995, 1143
Bsa29I ATCGAT 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 6 cut(s) 195, 613, 858, 914, 993, 1142
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 1142
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 164, 329, 561
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 3 cut(s) 246, 615, 1137
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 6 cut(s) 195, 613, 858, 914, 993, 1142
BseGI GGATG 4 cut(s) 63, 87, 191, 241
BseLI CCNNNNNNNGG 2 cut(s) 335, 1142
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 54, 943
BseNI ACTGG 3 cut(s) 164, 329, 561
BseRI GAGGAG 3 cut(s) 764, 767, 1169
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 1 cut(s) 919
Bsh1236I CGCG 1 cut(s) 1038
Bsh1285I CGRYCG 1 cut(s) 1015
BshFI GGCC 2 cut(s) 5, 1135
BshVI ATCGAT 1 cut(s) 1011
BsiEI CGRYCG 1 cut(s) 1015
BsiHKAI GWGCWC 2 cut(s) 283, 297
BsiHKCI CYCGRG 2 cut(s) 913, 1099
BsiSI CCGG 3 cut(s) 104, 995, 1142
BslFI GGGAC 2 cut(s) 314, 767
BslI CCNNNNNNNGG 2 cut(s) 335, 1142
BsmAI GTCTC 2 cut(s) 802, 924
BsmFI GGGAC 2 cut(s) 314, 767
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 2 cut(s) 5, 1135
Bso31I GGTCTC 1 cut(s) 924
BsoBI CYCGRG 2 cut(s) 913, 1099
Bsp1286I GDGCHC 3 cut(s) 283, 297, 670
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 5 cut(s) 325, 510, 538, 722, 1012
BspACI CCGC 1 cut(s) 1038
BspANI GGCC 2 cut(s) 5, 1135
BspCNI CTCAG 2 cut(s) 53, 942
BspDI ATCGAT 1 cut(s) 1011
BspFNI CGCG 1 cut(s) 1038
BspPI GGATC 2 cut(s) 320, 505
BspTNI GGTCTC 1 cut(s) 924
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 3 cut(s) 164, 329, 561
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 6 cut(s) 195, 613, 858, 914, 993, 1142
BssMI GATC 5 cut(s) 325, 510, 538, 722, 1012
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 246, 615, 1137
Bst4CI ACNGT 5 cut(s) 278, 621, 826, 862, 962
Bst6I CTCTTC 2 cut(s) 53, 420
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 3 cut(s) 224, 405, 596
BstDEI CTNAG 3 cut(s) 40, 470, 929
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 4 cut(s) 63, 87, 191, 241
BstFNI CGCG 1 cut(s) 1038
BstKTI GATC 5 cut(s) 328, 513, 541, 725, 1015
BstMAI GTCTC 2 cut(s) 802, 924
BstMBI GATC 5 cut(s) 325, 510, 538, 722, 1012
BstMCI CGRYCG 1 cut(s) 1015
BstNI CCWGG 3 cut(s) 246, 615, 1137
BstNSI RCATGY 1 cut(s) 226
BstPAI GACNNNNGTC 1 cut(s) 1144
BstSCI CCNGG 5 cut(s) 244, 613, 993, 1135, 1141
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 1 cut(s) 919
BstV2I GAAGAC 1 cut(s) 1088
BstX2I RGATCY 3 cut(s) 325, 538, 722
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 3 cut(s) 325, 538, 722
Bsu15I ATCGAT 1 cut(s) 1011
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 2 cut(s) 5, 1135
BsuTUI ATCGAT 1 cut(s) 1011
BtgI CCRYGG 1 cut(s) 858
BtgZI GCGATG 1 cut(s) 1019
BtsCI GGATG 4 cut(s) 63, 87, 191, 241
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 3 cut(s) 224, 405, 596
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 392, 1139
ClaI ATCGAT 1 cut(s) 1011
CseI GACGC 1 cut(s) 1093
Csp6I GTAC 3 cut(s) 229, 622, 736
CviAII CATG 6 cut(s) 208, 223, 268, 419, 515, 888
CviQI GTAC 3 cut(s) 229, 622, 736
DdeI CTNAG 3 cut(s) 40, 470, 929
DpnI GATC 5 cut(s) 327, 512, 540, 724, 1014
DpnII GATC 5 cut(s) 325, 510, 538, 722, 1012
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 53, 420
EarI CTCTTC 2 cut(s) 53, 420
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 392, 1139
Eco57I CTGAAG 1 cut(s) 828
Eco88I CYCGRG 2 cut(s) 913, 1099
EcoRII CCWGG 3 cut(s) 244, 613, 1135
EcoRV GATATC 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 6 cut(s) 211, 226, 271, 422, 518, 891
FaqI GGGAC 2 cut(s) 314, 767
FatI CATG 6 cut(s) 207, 222, 267, 418, 514, 887
Fnu4HI GCNGC 1 cut(s) 908
FokI GGATG 4 cut(s) 50, 74, 178, 228
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 1 cut(s) 908
FspBI CTAG 2 cut(s) 72, 1149
GluI GCNGC 1 cut(s) 908
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 5, 1135
HapII CCGG 3 cut(s) 104, 995, 1142
HgaI GACGC 1 cut(s) 1093
Hin1II CATG 6 cut(s) 211, 226, 271, 422, 518, 891
HincII GTYRAC 1 cut(s) 1170
HindII GTYRAC 1 cut(s) 1170
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 3 cut(s) 203, 927, 949
HpaI GTTAAC 1 cut(s) 1170
HpaII CCGG 3 cut(s) 104, 995, 1142
HphI GGTGA 1 cut(s) 795
Hpy166II GTNNAC 4 cut(s) 281, 708, 1019, 1170
Hpy188I TCNGA 6 cut(s) 481, 526, 640, 733, 841, 932
Hpy188III TCNNGA 7 cut(s) 436, 500, 720, 869, 982, 1016, 1198
Hpy8I GTNNAC 4 cut(s) 281, 708, 1019, 1170
HpyAV CCTTC 2 cut(s) 803, 884
HpyCH4III ACNGT 5 cut(s) 278, 621, 826, 862, 962
HpyCH4V TGCA 7 cut(s) 191, 281, 680, 741, 834, 1108, 1212
HpyF3I CTNAG 3 cut(s) 40, 470, 929
Hsp92II CATG 6 cut(s) 211, 226, 271, 422, 518, 891
KspAI GTTAAC 1 cut(s) 1170
Kzo9I GATC 5 cut(s) 325, 510, 538, 722, 1012
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 1 cut(s) 200
MaeI CTAG 2 cut(s) 72, 1149
MaeIII GTNAC 2 cut(s) 166, 379
MalI GATC 5 cut(s) 327, 512, 540, 724, 1014
MboI GATC 5 cut(s) 325, 510, 538, 722, 1012
MboII GAAGA 5 cut(s) 40, 299, 407, 954, 1093
MflI RGATCY 3 cut(s) 325, 538, 722
MhlI GDGCHC 3 cut(s) 283, 297, 670
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 250, 373, 544, 654, 711, 1043
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 921
MmeI TCCRAC 2 cut(s) 162, 554
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 77, 125, 491, 657, 1026, 1130, 1169
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 104, 995, 1142
MspR9I CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 3 cut(s) 246, 615, 1137
MvnI CGCG 1 cut(s) 1038
NciI CCSGG 2 cut(s) 995, 1143
NdeII GATC 5 cut(s) 325, 510, 538, 722, 1012
NlaIII CATG 6 cut(s) 211, 226, 271, 422, 518, 891
NmuCI GTSAC 2 cut(s) 166, 379
NspI RCATGY 1 cut(s) 226
PaeI GCATGC 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 1099
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 203, 949
PflMI CCANNNNNTGG 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 1 cut(s) 909
Ple19I CGATCG 1 cut(s) 1015
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
PshAI GACNNNNGTC 1 cut(s) 1144
Psp6I CCWGG 3 cut(s) 244, 613, 1135
PspGI CCWGG 3 cut(s) 244, 613, 1135
PspPI GGNCC 2 cut(s) 392, 1139
PspXI VCTCGAGB 1 cut(s) 1099
PsuI RGATCY 3 cut(s) 325, 538, 722
PvuI CGATCG 1 cut(s) 1015
RsaI GTAC 3 cut(s) 230, 623, 737
RsaNI GTAC 3 cut(s) 229, 622, 736
SaqAI TTAA 7 cut(s) 77, 125, 491, 657, 1026, 1130, 1169
SatI GCNGC 1 cut(s) 908
Sau3AI GATC 5 cut(s) 325, 510, 538, 722, 1012
Sau96I GGNCC 2 cut(s) 392, 1139
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 1 cut(s) 921
ScrFI CCNGG 5 cut(s) 246, 615, 995, 1137, 1143
SduI GDGCHC 3 cut(s) 283, 297, 670
SfaNI GCATC 1 cut(s) 200
Sfr274I CTCGAG 1 cut(s) 1099
SinI GGWCC 2 cut(s) 392, 1139
SlaI CTCGAG 1 cut(s) 1099
SmlI CTYRAG 3 cut(s) 436, 498, 1099
SmoI CTYRAG 3 cut(s) 436, 498, 1099
SphI GCATGC 1 cut(s) 226
Sse9I AATT 6 cut(s) 250, 373, 544, 654, 711, 1043
SsiI CCGC 1 cut(s) 1038
SspMI CTAG 2 cut(s) 72, 1149
StyD4I CCNGG 5 cut(s) 244, 613, 993, 1135, 1141
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 5 cut(s) 278, 621, 826, 862, 962
TaqI TCGA 3 cut(s) 973, 1011, 1100
TasI AATT 6 cut(s) 250, 373, 544, 654, 711, 1043
TatI WGTACW 2 cut(s) 621, 735
TfiI GAWTC 2 cut(s) 203, 949
Tru1I TTAA 7 cut(s) 77, 125, 491, 657, 1026, 1130, 1169
Tru9I TTAA 7 cut(s) 77, 125, 491, 657, 1026, 1130, 1169
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 2 cut(s) 166, 379
TseI GCWGC 1 cut(s) 907
Tsp45I GTSAC 2 cut(s) 166, 379
TspDTI ATGAA 2 cut(s) 224, 407
TspRI CASTG 1 cut(s) 171
Van91I CCANNNNNTGG 1 cut(s) 335
VneI GTGCAC 1 cut(s) 279
VpaK11BI GGWCC 2 cut(s) 392, 1139
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 1099
XspI CTAG 2 cut(s) 72, 1149
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.