pycom14g00820

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009545
Physical Location & Seq
Forward (+)
551086 .. 551526
441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00820.1

Sequence Viewer

Length: 441 bp
ATGGCCATAAAAGTTTATGTAATATCTTTCCTCCTAACTTCAGTTCTCTTCTCTCTCCTATACATCCCAACTAGGTTAAAAATCCCCATCCACAACTTCAAGCCAGTCATAAACTACTTCAACTTAACCACCGTTTCCAGCAACAGCACCACCAAGCCATATCCGGTGACATTTTCCTACCTAATCTCTGCATCCAAAGGCGATTCCATGAAGCTAAAGCGCATGCTGTATGCCCTATACCATCCTGGAAATTACTATCTAATCCATATGGATTACGCTGCACCAGAAGATGAGCACAGGGACATTGCTGACTTTGTGGCTGGATCGATCCAGTTTTTGGGCAAGTTGGTAATGTTTGGGTTTTGGGAAAACCTAATTTGGTCACTTACAGGGGACCAACCATGCTTGCCACTACTCTTCATGCATTGTCTTTGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

147

Amino Acids

16.74

Weight (kDa)

8.41

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 104 1.8e-06 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AclWI GGATC 2 cut(s) 322, 331
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 337
AgsI TTSAA 2 cut(s) 100, 121
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 2 cut(s) 322, 331
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 265
BccI CCATC 2 cut(s) 95, 249
BciT130I CCWGG 1 cut(s) 246
BfaI CTAG 1 cut(s) 72
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 326
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 104, 331
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 246
BseCI ATCGAT 1 cut(s) 326
BseGI GGATG 4 cut(s) 63, 87, 191, 241
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 303
BseNI ACTGG 2 cut(s) 104, 331
BseXI GCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 2 cut(s) 314, 407
BslI CCNNNNNNNGG 1 cut(s) 337
BsmFI GGGAC 2 cut(s) 314, 407
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 323, 327
BspANI GGCC 1 cut(s) 5
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 395
BspPI GGATC 2 cut(s) 322, 331
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 2 cut(s) 104, 331
BssMI GATC 2 cut(s) 323, 327
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 53, 422
BstC8I GCNNGC 2 cut(s) 224, 407
BstF5I GGATG 4 cut(s) 63, 87, 191, 241
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 326, 330
BstMBI GATC 2 cut(s) 323, 327
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 326
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 326
BtsCI GGATG 4 cut(s) 63, 87, 191, 241
Cac8I GCNNGC 2 cut(s) 224, 407
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 394
ClaI ATCGAT 1 cut(s) 326
CviAII CATG 4 cut(s) 208, 223, 402, 421
CviJI RGCY 5 cut(s) 5, 103, 157, 214, 320
CviKI_1 RGCY 5 cut(s) 5, 103, 157, 214, 320
DpnI GATC 2 cut(s) 325, 329
DpnII GATC 2 cut(s) 323, 327
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 53, 422
EarI CTCTTC 2 cut(s) 53, 422
Eco47I GGWCC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 426
FaeI CATG 4 cut(s) 211, 226, 405, 424
FaqI GGGAC 2 cut(s) 314, 407
FatI CATG 4 cut(s) 207, 222, 401, 420
FauNDI CATATG 1 cut(s) 267
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 4 cut(s) 50, 74, 178, 228
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 72
GlaI GCGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 164
HhaI GCGC 1 cut(s) 222
Hin1II CATG 4 cut(s) 211, 226, 405, 424
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 164
HphI GGTGA 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 438
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 3 cut(s) 191, 281, 424
Hsp92II CATG 4 cut(s) 211, 226, 405, 424
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 323, 327
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 1 cut(s) 200
MaeI CTAG 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 166, 381
MalI GATC 2 cut(s) 325, 329
MboI GATC 2 cut(s) 323, 327
MboII GAAGA 3 cut(s) 40, 299, 409
MhlI GDGCHC 1 cut(s) 297
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 250, 375
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 41
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 426
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 77, 125
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 267
NdeII GATC 2 cut(s) 323, 327
NlaIII CATG 4 cut(s) 211, 226, 405, 424
NlaIV GGNNCC 1 cut(s) 395
NmuCI GTSAC 2 cut(s) 166, 381
NsiI ATGCAT 1 cut(s) 426
NspI RCATGY 1 cut(s) 226
PaeI GCATGC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 1 cut(s) 394
SaqAI TTAA 2 cut(s) 77, 125
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 2 cut(s) 323, 327
Sau96I GGNCC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 297
SetI ASST 4 cut(s) 77, 183, 216, 375
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 1 cut(s) 394
SphI GCATGC 1 cut(s) 226
Sse9I AATT 2 cut(s) 250, 375
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 133
TaqI TCGA 1 cut(s) 326
TasI AATT 2 cut(s) 250, 375
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 2 cut(s) 77, 125
Tru9I TTAA 2 cut(s) 77, 125
TseFI GTSAC 2 cut(s) 166, 381
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 2 cut(s) 166, 381
TspDTI ATGAA 2 cut(s) 224, 409
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 394
XceI RCATGY 1 cut(s) 226
XspI CTAG 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.