AT3G05320

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006563
Physical Location & Seq
Reverse (-)
1513062 .. 1515568
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05320.2

Sequence Viewer

Length: 1338 bp
ATGAATTCTCCGTTAAGTTCTAAGAATCTGAGAATCTTTTCAAAGTCTGTTGCTTGCAAATGTCTTGTTCTTGTTGGTATTGCTCTGTTCTACAGAGCTCTGTTACTCTCTTACTCTCCCAGAAACGCTCTGAGCAATAGCTTATTGTTTCGAGATCGTCACATGTCAGATTCCTCCTCAACCGGTGGAATCCGAACTGATAAGTTTCTCGAGGTTCCTCAGATCGTCTGGGGACTAAACAATCAGAAGATAGCTTTTGCTAGAGCTTGCTTGACGGCAAGAATGATGAACAGAACGCTTCTCATGCCTAGTCTAAGTGCTTCCTTGTTCTACAAGGAAGTTGATAAGCTTCGTCCCATTCCGTTTGATAAAGTGTTTCAGTTTGAAAGATTCAACTCTCTCTGTTCAGGGTTTGTGCGGCTTTCTCGGTTCTCGGATGTTAAAAACAGAGCACAAGTGTTTGATCTAGAGAAAGGTAGTGGAAGAAGATGGACGGTTGAAAGAGACTTGGAACACTTGAAACAATCCGCTCGTAACGAGTCCATCGATGAGTTTGAAGTGATCCGTCTCATAGGGAAAAACCCGTTCTTGTGGCACGATCATTGGCCTGTTGAAGACTATGCTAAGGTCTTTGAGTGTATGGTGGTGGTTGATGAAATCTCAAGAGAAGCAGATAAGGTTGTGATGAAAATCCGAGAAGCAGGTGAGGCAGAAAGGGCAAAACTCGCATCGAAAACCGAAATCCTCGGTCCGGTACCTTTTGTTGCGGTTCACATGAGAATAGAGATAGATTGGATGATACATTGTAAGAAACTAGAGCAACGGAAAAAGATCTCAGAGATTTGTAGTAGTAAAAGAGAGATTTTGGACAGAGTAGGGAACATTTCAGGTTTAAAGACTCCAACAGTTCTTTACCTTGCAGTAGCAGATACTCTCTTAGAAGAAAAAGAAGAAGATTCATCGGTTTTAAGTGGTTGGAGAGACGGTTTAATACCATTTGAGAAGAAGAAGCTTGGGGTTAAAGAGGAGATATATGGGAAGTATTCATATTTGATACAATCGGCTATAGATTACGAGGTGTGTTTAAGAGCGGATGTTTTTGTTGGTAATAGCTTTTCGACTTTCTCTAGTCTCATTGTTCTTGAGAGGACACAGAAAGCGAGAAGGTTAGGGTTTATGAGTTCTTGTAAAGATGGTGGAAACAAGTGGAGATCTTATGCATATAATCTGGCTGGTGAATCAAACGGAGTTCCAAGGAGATGGATGACAAATATGACTCATTCGAGCTTGCAAGCTATTAGCTATGGCTCAAATTCTGTTTCTTGTTCTAGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

445

Amino Acids

50.65

Weight (kDa)

9.24

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 78 - 384 1.5e-25 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
Acc36I ACCTGC 1 cut(s) 692
Acc65I GGTACC 1 cut(s) 754
AccB1I GGYRCC 1 cut(s) 754
AccBSI CCGCTC 2 cut(s) 530, 1091
AciI CCGC 4 cut(s) 418, 528, 767, 1091
AclWI GGATC 1 cut(s) 556
AcsI RAATTY 2 cut(s) 4, 1312
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 1 cut(s) 751
AflIII ACRYGT 1 cut(s) 162
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 7 cut(s) 42, 386, 394, 500, 520, 557, 614
Alw21I GWGCWC 2 cut(s) 100, 454
Alw26I GTCTC 4 cut(s) 498, 572, 975, 1136
AlwI GGATC 1 cut(s) 556
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 605
ApoI RAATTY 2 cut(s) 4, 1312
AsiGI ACCGGT 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 37
Asp718I GGTACC 1 cut(s) 754
AspS9I GGNCC 1 cut(s) 749
AsuHPI GGTGA 2 cut(s) 716, 1247
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 2 cut(s) 100, 454
BccI CCATC 4 cut(s) 483, 551, 1187, 1254
BceAI ACGGC 1 cut(s) 291
BcoDI GTCTC 4 cut(s) 498, 572, 975, 1136
BfaI CTAG 6 cut(s) 261, 309, 467, 815, 1128, 1329
BfmI CTRYAG 2 cut(s) 91, 1065
BfuAI ACCTGC 1 cut(s) 692
BglII AGATCT 2 cut(s) 831, 1211
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 749
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 2 cut(s) 216, 756
BmsI GCATC 1 cut(s) 737
BpiI GAAGAC 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 646, 1163
Bsa29I ATCGAT 1 cut(s) 546
BsaBI GATNNNNATC 1 cut(s) 689
BsaJI CCNNGG 2 cut(s) 745, 1253
BsaWI WCCGGW 2 cut(s) 182, 751
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bsc4I CCNNNNNNNGG 1 cut(s) 751
Bse118I RCCGGY 1 cut(s) 182
Bse8I GATNNNNATC 1 cut(s) 689
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 2 cut(s) 745, 1253
BseGI GGATG 4 cut(s) 442, 801, 1099, 1269
BseJI GATNNNNATC 1 cut(s) 689
BseLI CCNNNNNNNGG 1 cut(s) 751
BseMII CTCAG 4 cut(s) 20, 122, 233, 849
BseRI GAGGAG 2 cut(s) 166, 1040
BshFI GGCC 1 cut(s) 607
BshNI GGYRCC 1 cut(s) 754
BshTI ACCGGT 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 546
BsiHKAI GWGCWC 2 cut(s) 100, 454
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 2 cut(s) 183, 752
BslFI GGGAC 2 cut(s) 246, 339
BslI CCNNNNNNNGG 1 cut(s) 751
BsmAI GTCTC 4 cut(s) 498, 572, 975, 1136
BsmBI CGTCTC 2 cut(s) 572, 975
BsmFI GGGAC 2 cut(s) 246, 339
BsnI GGCC 1 cut(s) 607
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 100, 454
Bsp143I GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
BspACI CCGC 4 cut(s) 418, 528, 767, 1091
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 4 cut(s) 21, 123, 232, 848
BspDI ATCGAT 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 216, 756
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 754
BsrBI CCGCTC 2 cut(s) 530, 1091
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 745, 1253
BssMI GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
BssT1I CCWWGG 1 cut(s) 1253
Bst4CI ACNGT 3 cut(s) 496, 907, 986
BstC8I GCNNGC 4 cut(s) 55, 268, 1289, 1293
BstDEI CTNAG 8 cut(s) 21, 29, 131, 219, 314, 624, 835, 937
BstF5I GGATG 4 cut(s) 442, 801, 1099, 1269
BstKTI GATC 7 cut(s) 157, 225, 466, 564, 601, 834, 1214
BstMAI GTCTC 4 cut(s) 498, 572, 975, 1136
BstMBI GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
BstMWI GCNNNNNNNGC 4 cut(s) 304, 707, 716, 725
BstNSI RCATGY 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 91, 1065
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 2 cut(s) 831, 1211
BstXI CCANNNNNNTGG 1 cut(s) 1260
BstYI RGATCY 2 cut(s) 831, 1211
Bsu15I ATCGAT 1 cut(s) 546
BsuRI GGCC 1 cut(s) 607
BsuTUI ATCGAT 1 cut(s) 546
BtsCI GGATG 4 cut(s) 442, 801, 1099, 1269
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 4 cut(s) 55, 268, 1289, 1293
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 749
ClaI ATCGAT 1 cut(s) 546
CpoI CGGWCCG 1 cut(s) 749
Csp6I GTAC 1 cut(s) 755
CspAI ACCGGT 1 cut(s) 182
CspI CGGWCCG 1 cut(s) 749
CviAII CATG 3 cut(s) 163, 304, 775
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 8 cut(s) 21, 29, 131, 219, 314, 624, 835, 937
DpnI GATC 7 cut(s) 156, 224, 465, 563, 600, 833, 1213
DpnII GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
DraI TTTAAA 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 1253
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 1253
EcoT22I ATGCAT 1 cut(s) 1222
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 1253
Esp3I CGTCTC 2 cut(s) 572, 975
FaeI CATG 3 cut(s) 166, 307, 778
FaqI GGGAC 2 cut(s) 246, 339
FatI CATG 3 cut(s) 162, 303, 774
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 4 cut(s) 449, 808, 1106, 1276
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 6 cut(s) 261, 309, 467, 815, 1128, 1329
GluI GCNGC 1 cut(s) 419
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 183, 752
Hin1II CATG 3 cut(s) 166, 307, 778
HindIII AAGCTT 2 cut(s) 347, 1010
HpaII CCGG 2 cut(s) 183, 752
HphI GGTGA 2 cut(s) 716, 1247
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 9 cut(s) 30, 132, 169, 194, 222, 246, 436, 695, 838
Hpy188III TCNNGA 5 cut(s) 152, 209, 467, 663, 1142
Hpy8I GTNNAC 1 cut(s) 772
HpyAV CCTTC 1 cut(s) 1158
HpyCH4III ACNGT 3 cut(s) 496, 907, 986
HpyCH4V TGCA 4 cut(s) 57, 920, 1220, 1291
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 707, 716, 725
HpyF3I CTNAG 8 cut(s) 21, 29, 131, 219, 314, 624, 835, 937
Hsp92II CATG 3 cut(s) 166, 307, 778
KpnI GGTACC 1 cut(s) 758
Kzo9I GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
LweI GCATC 1 cut(s) 737
MaeI CTAG 6 cut(s) 261, 309, 467, 815, 1128, 1329
MaeIII GTNAC 3 cut(s) 102, 158, 533
MalI GATC 7 cut(s) 156, 224, 465, 563, 600, 833, 1213
MbiI CCGCTC 2 cut(s) 530, 1091
MboI GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
MboII GAAGA 9 cut(s) 259, 495, 498, 626, 953, 962, 965, 1015, 1018
MflI RGATCY 2 cut(s) 831, 1211
MhlI GDGCHC 2 cut(s) 100, 454
MluCI AATT 2 cut(s) 4, 1312
MlyI GAGTC 3 cut(s) 548, 892, 1270
MmeI TCCRAC 2 cut(s) 926, 956
MnlI CCTC 9 cut(s) 184, 187, 205, 228, 700, 755, 1018, 1069, 1140
Mph1103I ATGCAT 1 cut(s) 1222
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 7 cut(s) 14, 441, 893, 968, 989, 1020, 1085
MspI CCGG 2 cut(s) 183, 752
MwoI GCNNNNNNNGC 4 cut(s) 304, 707, 716, 725
NdeII GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
NlaIII CATG 3 cut(s) 166, 307, 778
NlaIV GGNNCC 2 cut(s) 216, 756
NmuCI GTSAC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 1222
NspI RCATGY 1 cut(s) 166
PaeR7I CTCGAG 1 cut(s) 209
PaqCI CACCTGC 1 cut(s) 692
PciI ACATGT 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 7 cut(s) 25, 33, 170, 189, 390, 956, 1238
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 1 cut(s) 420
PleI GAGTC 3 cut(s) 547, 892, 1270
PpsI GAGTC 3 cut(s) 547, 892, 1270
PscI ACATGT 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 216, 756
PspPI GGNCC 1 cut(s) 749
PsuI RGATCY 2 cut(s) 831, 1211
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
Rsr2I CGGWCCG 1 cut(s) 749
RsrII CGGWCCG 1 cut(s) 749
SacI GAGCTC 1 cut(s) 100
SaqAI TTAA 7 cut(s) 14, 441, 893, 968, 989, 1020, 1085
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 7 cut(s) 154, 222, 463, 561, 598, 831, 1211
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 3 cut(s) 548, 892, 1270
SduI GDGCHC 2 cut(s) 100, 454
SfaNI GCATC 1 cut(s) 737
SfcI CTRYAG 2 cut(s) 91, 1065
Sfr274I CTCGAG 1 cut(s) 209
SinI GGWCC 1 cut(s) 749
SlaI CTCGAG 1 cut(s) 209
SmlI CTYRAG 3 cut(s) 209, 661, 1142
SmoI CTYRAG 3 cut(s) 209, 661, 1142
Sse9I AATT 2 cut(s) 4, 1312
SsiI CCGC 4 cut(s) 418, 528, 767, 1091
SspMI CTAG 6 cut(s) 261, 309, 467, 815, 1128, 1329
SstI GAGCTC 1 cut(s) 100
StyI CCWWGG 1 cut(s) 1253
TaaI ACNGT 3 cut(s) 496, 907, 986
TaqI TCGA 6 cut(s) 151, 210, 546, 731, 1118, 1283
TaqII GACCGA 1 cut(s) 737
TasI AATT 2 cut(s) 4, 1312
TauI GCSGC 1 cut(s) 421
TfiI GAWTC 7 cut(s) 25, 33, 170, 189, 390, 956, 1238
Tru1I TTAA 7 cut(s) 14, 441, 893, 968, 989, 1020, 1085
Tru9I TTAA 7 cut(s) 14, 441, 893, 968, 989, 1020, 1085
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 6 cut(s) 17, 302, 669, 701, 948, 1035
TspGWI ACGGA 4 cut(s) 351, 554, 838, 1260
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 2 cut(s) 4, 1312
XbaI TCTAGA 1 cut(s) 466
XceI RCATGY 1 cut(s) 166
XhoI CTCGAG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 6 cut(s) 261, 309, 467, 815, 1128, 1329
Zsp2I ATGCAT 1 cut(s) 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.