RchiOBHm_Chr5g0019601

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006563
Physical Location & Seq
Forward (+)
14064439 .. 14066105
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29973

Sequence Viewer

Length: 651 bp
ATGAGTTTCTCGAGGTTCCTCAAATTGTATGGGGATCAATGTTTTAAAAGGCTAAGGCGTAGCCGGGAGAGCCTTAGCAAGGCGCTTGCCTTGAGGCGTAAGGCGACTCTTTTGATGCCTAGCTTGAGTGCTTCCTTGTTCTACAAAGAAGTTGAGCTCTTGAGGCCCATTTCGTTTGATCATTGGCCTGTGAAGGACCATTCAGAGGTGTTTGAGTGCTTGGTTCTGGCTGATGAGATCATGAGAGAAGCCGATAGAGTTATGTCAAGGATTAGAGAGATAGGAGCAGAACAAGTGAGAAGCAAAAGAGAGTCTGCGGAGAACTCTTTGTTGCTCAAGTCAGTGCCTTATGTAGCTGTTCATATGAGGATAGAGTTAGATTGGATGATACACTGTAAGAATCTCGAGCGTAGATTGAACATAACCGATATTTGTAGCAGAAAGGGGGAGATCATGGAGAGAGTCGGCAACATTGTTGGCCTCAAGACTCCAGCTGTTGTTTATTTAGCTGTGGCTGATAGTCTTCTTCAAGATCCATCAATTTTGAATGGATGGCGGGAAAGAATGATTCCTTTTGAGAAGAAGAAACTGGGTTTTGATGCACGTTACAAGAAGTTCCCCTATCTCATTCAGTCTGCGATTGACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

216

Amino Acids

25.24

Weight (kDa)

9.53

Isoelectric Point (pI)

60.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 47 - 136 3.5e-06 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 317, 556
AclWI GGATC 2 cut(s) 42, 527
AfiI CCNNNNNNNGG 2 cut(s) 79, 205
AgsI TTSAA 3 cut(s) 418, 530, 547
AluBI AGCT 5 cut(s) 123, 157, 356, 494, 509
AluI AGCT 5 cut(s) 123, 157, 356, 494, 509
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 2 cut(s) 42, 527
Ama87I CYCGRG 2 cut(s) 10, 404
AoxI GGCC 3 cut(s) 164, 185, 478
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 2 cut(s) 165, 196
AsuC2I CCSGG 1 cut(s) 65
AvaI CYCGRG 2 cut(s) 10, 404
AvaII GGWCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 159
BccI CCATC 2 cut(s) 544, 546
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 1 cut(s) 65
BfaI CTAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 86
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 2 cut(s) 10, 404
BmgT120I GGNCC 2 cut(s) 165, 196
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 2 cut(s) 105, 589
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 2 cut(s) 53, 74
BpuEI CTTGAG 5 cut(s) 112, 145, 181, 320, 467
BpuMI CCSGG 1 cut(s) 65
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 205
Bse1I ACTGG 1 cut(s) 594
BseGI GGATG 2 cut(s) 390, 557
BseLI CCNNNNNNNGG 2 cut(s) 79, 205
BseNI ACTGG 1 cut(s) 594
BshFI GGCC 3 cut(s) 166, 187, 480
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 2 cut(s) 10, 404
BsiSI CCGG 1 cut(s) 64
BslI CCNNNNNNNGG 2 cut(s) 79, 205
BsnI GGCC 3 cut(s) 166, 187, 480
BsoBI CYCGRG 2 cut(s) 10, 404
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 5 cut(s) 34, 178, 237, 450, 532
BspACI CCGC 2 cut(s) 317, 556
BspANI GGCC 3 cut(s) 166, 187, 480
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 17
BspPI GGATC 2 cut(s) 42, 527
BsrI ACTGG 1 cut(s) 594
BssMI GATC 5 cut(s) 34, 178, 237, 450, 532
Bst4CI ACNGT 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 53, 74
BstENI CCTNNNNNAGG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 390, 557
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 5 cut(s) 37, 181, 240, 453, 535
BstMBI GATC 5 cut(s) 34, 178, 237, 450, 532
BstMWI GCNNNNNNNGC 2 cut(s) 69, 163
BstSCI CCNGG 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 3 cut(s) 166, 187, 480
BtsCI GGATG 2 cut(s) 390, 557
BtsIMutI CAGTG 2 cut(s) 348, 391
Cac8I GCNNGC 1 cut(s) 87
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 2 cut(s) 165, 196
CviAII CATG 2 cut(s) 241, 454
DdeI CTNAG 2 cut(s) 53, 74
DpnI GATC 5 cut(s) 36, 180, 239, 452, 534
DpnII GATC 5 cut(s) 34, 178, 237, 450, 532
DraI TTTAAA 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 157
Eco88I CYCGRG 2 cut(s) 10, 404
EcoICRI GAGCTC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 77
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 2 cut(s) 244, 457
FaiI YATR 8 cut(s) 30, 242, 263, 351, 363, 365, 422, 455
FatI CATG 2 cut(s) 240, 453
FauI CCCGC 1 cut(s) 549
FauNDI CATATG 1 cut(s) 363
FbaI TGATCA 1 cut(s) 178
FokI GGATG 2 cut(s) 397, 564
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 1 cut(s) 120
GlaI GCGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 474
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 166, 187, 480
HapII CCGG 1 cut(s) 64
HhaI GCGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 244, 457
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HinfI GANTC 6 cut(s) 106, 311, 400, 462, 487, 568
HpaII CCGG 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 6 cut(s) 10, 160, 241, 404, 484, 530
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 604
HpyCH4V TGCA 1 cut(s) 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 163
HpyF3I CTNAG 2 cut(s) 53, 74
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 2 cut(s) 244, 457
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 5 cut(s) 34, 178, 237, 450, 532
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 5 cut(s) 77, 201, 212, 504, 575
LweI GCATC 2 cut(s) 105, 589
MaeI CTAG 1 cut(s) 120
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 1 cut(s) 605
MalI GATC 5 cut(s) 36, 180, 239, 452, 534
MboI GATC 5 cut(s) 34, 178, 237, 450, 532
MboII GAAGA 4 cut(s) 515, 518, 592, 595
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 2 cut(s) 23, 540
MlyI GAGTC 4 cut(s) 100, 320, 471, 481
MnlI CCTC 7 cut(s) 6, 29, 87, 156, 199, 360, 491
MseI TTAA 2 cut(s) 45, 649
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 69, 163
NciI CCSGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 363
NdeII GATC 5 cut(s) 34, 178, 237, 450, 532
NlaIII CATG 2 cut(s) 244, 457
NlaIV GGNNCC 1 cut(s) 17
PaeR7I CTCGAG 2 cut(s) 10, 404
PagI TCATGA 1 cut(s) 240
PfeI GAWTC 2 cut(s) 400, 568
PleI GAGTC 4 cut(s) 100, 319, 470, 481
PpsI GAGTC 4 cut(s) 100, 319, 470, 481
Psp124BI GAGCTC 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 17
PspPI GGNCC 2 cut(s) 165, 196
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 45, 649
Sau3AI GATC 5 cut(s) 34, 178, 237, 450, 532
Sau96I GGNCC 2 cut(s) 165, 196
SchI GAGTC 4 cut(s) 100, 320, 471, 481
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 1 cut(s) 159
SetI ASST 8 cut(s) 17, 125, 159, 210, 358, 496, 511, 607
SfaNI GCATC 2 cut(s) 105, 589
Sfr274I CTCGAG 2 cut(s) 10, 404
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 2 cut(s) 10, 404
SmlI CTYRAG 7 cut(s) 10, 91, 124, 160, 335, 404, 482
SmoI CTYRAG 7 cut(s) 10, 91, 124, 160, 335, 404, 482
Sse9I AATT 2 cut(s) 23, 540
SsiI CCGC 2 cut(s) 317, 556
SspMI CTAG 1 cut(s) 120
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 607
TaqI TCGA 2 cut(s) 11, 405
TasI AATT 2 cut(s) 23, 540
TfiI GAWTC 2 cut(s) 400, 568
Tru1I TTAA 2 cut(s) 45, 649
Tru9I TTAA 2 cut(s) 45, 649
TscAI CASTG 2 cut(s) 348, 398
TspDTI ATGAA 1 cut(s) 350
TspRI CASTG 2 cut(s) 348, 398
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 1 cut(s) 77
XhoI CTCGAG 2 cut(s) 10, 404
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.