MD10G1236200.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006563
Physical Location & Seq
Reverse (-)
33273330 .. 33275647
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1236200.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGGACGTGCCTCCTTACTGTAAGAATTTCAAAGTTCTCGGTTGCCATTTGAATTCGTTCGCATGCAAATGTGTTGTTTTAGCTGTGACTGCTCTGGTAATCAGAGCTGTTCTGCTTCCTACGTTCTCCATCTTTGATGGAACTGAACAGGACAACTTGGTATTCATCGGTAATCTGTCGTTGTCATTCGGTCAGAAATCTGGAATCCGAAAAGATAAGTTTCTGGAGGTTCCTCAAATCGTTTGGGGACTAAACAATCAGAAAATAGCGTTTGCAAGAGCTTGTCTGACCGCAAGGCTTTTGAACCGGACGCTTTTGATGCCTAGCTTGAGTGCTTCCCTGTTTTACAAGGAAGTAGAGCTCTTGGAGCCGATTTCCTTCGATAAGGTGTTCCAGTTCAAGAAGTTTAATTCTCTCTGTAATGGATTCGTTCAATTGGGTGAACTTTCAGATGTTAGAAACCGAACAGGAGCATTTGAGCTTCAGAAAGGAAGCGGAAGGAGGTGGACTCCCGAGAGGGACATGGATCAATTGAAACAGCATAATGAGGATCCATACAATGAGTACGAGGTGATTCAAATAGTAGGAAAGAATCCGTTTCTGTGGCACGATCATTGGCCTGTGAAGGACTATGCGAAAATCTTTGAGTGCATGGTTTTGGTTGATGAGATAATGAATGAAGCGGATAAAGTCGTGTCACGGATTAGAGAGATCGGAGCAGCACAAGTAAGTAGTCGGAAAGAGTTTGCGCAAATTGGCATTAGCCCGGCAGAGAATTCTGTCGTGCAGCCGGTGCCTTATGTGGCTGTACACATGAGGATAGAGATAGATTGGATGATTCATTGTAAGAAGCTAGAGCAAAGATCAAACATAACCCAAATTTGTAGTAGCAAGGAGGAGATCATGGAAAGAGTAGGCAACATTGTTGGCCTCAAAACGCCTGTCGTTGTTTATTTAGCTATAGCTGATAGCCTTCTTCATGATCCGTCCATTCTAAACGGCTGGAAAGAAGGCCTGATTCCTTTCGAGAAGAAGAAATTGGGCGTCGAAGGAAATTACAAGAAGTTCTCTTATCTCATTCAGTCTGCAATCGACTACGAAGTGTGTTTAAGGGCTGATGTGTTTGTGGGAAACAGTTTCTCGACATTTTCGAATCTTATAGTTCTGGATCGAACGCAGAAGCTCTTAAGAACGGGCGTGACAAGCTCATGTGGTGTGGATGTAAGGTGGCCTTCTTATGCATACAACATATTAGGGGAATCCAAGGGCCCTCAACGATGGATGACAAATATGTCTGGCTCAAGTCTTCAAGCAATTAGCTATGGCTCCAACGACATCTCCTGTCGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

451

Amino Acids

50.88

Weight (kDa)

8.34

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 83 - 391 4.6e-24 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1291
Acc16I TGCGCA 1 cut(s) 750
AccB1I GGYRCC 1 cut(s) 793
AciI CCGC 3 cut(s) 291, 495, 683
AclWI GGATC 5 cut(s) 534, 545, 558, 977, 1176
AcsI RAATTY 4 cut(s) 25, 52, 775, 879
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 1044
AfaI GTAC 2 cut(s) 566, 810
AflII CTTAAG 1 cut(s) 1186
AgsI TTSAA 8 cut(s) 31, 52, 304, 400, 434, 535, 578, 1310
AjiI CACGTC 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
Alw21I GWGCWC 1 cut(s) 363
AlwI GGATC 5 cut(s) 534, 545, 558, 977, 1176
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 5 cut(s) 617, 928, 1012, 1229, 1267
ApaI GGGCCC 1 cut(s) 1271
ApeKI GCWGC 2 cut(s) 719, 787
ApoI RAATTY 4 cut(s) 25, 52, 775, 879
ArsI GACNNNNNNTTYG 2 cut(s) 927, 959
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 2 cut(s) 1267, 1268
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 452, 583
AsuII TTCGAA 1 cut(s) 1151
AvaI CYCGRG 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 1271
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 793
BanII GRGCYC 2 cut(s) 363, 1271
BbsI GAAGAC 1 cut(s) 1298
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 731, 799
BccI CCATC 3 cut(s) 131, 137, 1272
BceAI ACGGC 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 767
BfaI CTAG 2 cut(s) 324, 854
BfmI CTRYAG 1 cut(s) 960
BfrI CTTAAG 1 cut(s) 1186
BisI GCNGC 2 cut(s) 720, 788
BlsI GCNGC 2 cut(s) 721, 789
Bme1390I CCNGG 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 1267, 1268
BmiI GGNNCC 6 cut(s) 231, 369, 552, 795, 1269, 1327
BmrFI CCNGG 1 cut(s) 767
BmsI GCATC 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 1298
BplI GAGNNNNNCTC 2 cut(s) 493, 525
BpmI CTGGAG 1 cut(s) 245
Bpu14I TTCGAA 1 cut(s) 1151
BpuEI CTTGAG 2 cut(s) 349, 1285
BpuMI CCSGG 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 1044
BsaJI CCNNGG 1 cut(s) 1263
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 1322, 1352
Bse118I RCCGGY 1 cut(s) 790
Bse1I ACTGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 1263
BseGI GGATG 3 cut(s) 840, 1225, 1287
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 911
BseSI GKGCMC 1 cut(s) 1271
BseXI GCAGC 2 cut(s) 731, 799
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BshNI GGYRCC 1 cut(s) 793
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 307, 767, 791
BslFI GGGAC 2 cut(s) 261, 533
BsmFI GGGAC 2 cut(s) 261, 533
BsnI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 1151
Bsp120I GGGCCC 1 cut(s) 1267
Bsp1286I GDGCHC 2 cut(s) 363, 1271
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
BspACI CCGC 3 cut(s) 291, 495, 683
BspANI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 6 cut(s) 231, 369, 552, 795, 1269, 1327
BspPI GGATC 5 cut(s) 534, 545, 558, 977, 1176
BspT104I TTCGAA 1 cut(s) 1151
BspT107I GGYRCC 1 cut(s) 793
BspTI CTTAAG 1 cut(s) 1186
BsrFI RCCGGY 1 cut(s) 790
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 790
BssECI CCNNGG 1 cut(s) 1263
BssMI GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
BssNI GRCGYC 1 cut(s) 1044
BssT1I CCWWGG 1 cut(s) 1263
Bst4CI ACNGT 2 cut(s) 20, 1136
BstACI GRCGYC 1 cut(s) 1044
BstAFI CTTAAG 1 cut(s) 1186
BstAPI GCANNNNNTGC 1 cut(s) 793
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 1151
BstC8I GCNNGC 1 cut(s) 64
BstF5I GGATG 3 cut(s) 840, 1225, 1287
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 8 cut(s) 529, 553, 613, 714, 866, 903, 985, 1171
BstMBI GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
BstMWI GCNNNNNNNGC 5 cut(s) 89, 319, 367, 793, 1203
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 765
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 1 cut(s) 1271
BstV1I GCAGC 2 cut(s) 731, 799
BstV2I GAAGAC 1 cut(s) 1298
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 3 cut(s) 840, 1225, 1287
Cac8I GCNNGC 1 cut(s) 64
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 790
Cfr13I GGNCC 2 cut(s) 1267, 1268
CseI GACGC 2 cut(s) 319, 1033
Csp6I GTAC 2 cut(s) 565, 809
CviAII CATG 7 cut(s) 63, 523, 652, 814, 904, 980, 1209
CviQI GTAC 2 cut(s) 565, 809
DpnI GATC 8 cut(s) 528, 552, 612, 713, 865, 902, 984, 1170
DpnII GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
DrdI GACNNNNNNGTC 1 cut(s) 1291
DseDI GACNNNNNNGTC 1 cut(s) 1291
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 1263
Eco147I AGGCCT 1 cut(s) 1014
Eco24I GRGCYC 2 cut(s) 363, 1271
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 1267, 1268
EcoRI GAATTC 2 cut(s) 52, 775
EcoT14I CCWWGG 1 cut(s) 1263
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 363, 1271
ErhI CCWWGG 1 cut(s) 1263
FaeI CATG 7 cut(s) 66, 526, 655, 817, 907, 983, 1212
FalI AAGNNNNNCTT 2 cut(s) 1216, 1248
FaqI GGGAC 2 cut(s) 261, 533
FatI CATG 7 cut(s) 62, 522, 651, 813, 903, 979, 1208
Fnu4HI GCNGC 2 cut(s) 720, 788
FokI GGATG 3 cut(s) 847, 1232, 1294
FriOI GRGCYC 2 cut(s) 363, 1271
Fsp4HI GCNGC 2 cut(s) 720, 788
FspBI CTAG 2 cut(s) 324, 854
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 1 cut(s) 750
GluI GCNGC 2 cut(s) 720, 788
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
HapII CCGG 3 cut(s) 307, 767, 791
HgaI GACGC 2 cut(s) 319, 1033
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 1 cut(s) 1044
Hin1II CATG 7 cut(s) 66, 526, 655, 817, 907, 983, 1212
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HpaII CCGG 3 cut(s) 307, 767, 791
HphI GGTGA 2 cut(s) 452, 583
Hpy166II GTNNAC 3 cut(s) 443, 507, 811
Hpy188III TCNNGA 8 cut(s) 201, 224, 400, 512, 980, 1027, 1141, 1166
Hpy8I GTNNAC 3 cut(s) 443, 507, 811
Hpy99I CGWCG 1 cut(s) 1049
HpyAV CCTTC 7 cut(s) 388, 492, 619, 983, 1004, 1043, 1242
HpyCH4III ACNGT 2 cut(s) 20, 1136
HpyCH4IV ACGT 2 cut(s) 6, 122
HpyCH4V TGCA 6 cut(s) 66, 275, 651, 787, 1088, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 319, 367, 793, 1203
HpySE526I ACGT 2 cut(s) 6, 122
Hsp92I GRCGYC 1 cut(s) 1044
Hsp92II CATG 7 cut(s) 66, 526, 655, 817, 907, 983, 1212
HspAI GCGC 1 cut(s) 749
Kzo9I GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
LmnI GCTCC 4 cut(s) 367, 470, 716, 1331
Lsp1109I GCAGC 2 cut(s) 731, 799
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 324, 854
MaeII ACGT 2 cut(s) 6, 122
MaeIII GTNAC 3 cut(s) 85, 696, 1198
MalI GATC 8 cut(s) 528, 552, 612, 713, 865, 902, 984, 1170
MboI GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
MboII GAAGA 4 cut(s) 968, 1042, 1045, 1298
MfeI CAATTG 2 cut(s) 434, 530
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 363, 1271
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 2 cut(s) 716, 1353
Mph1103I ATGCAT 1 cut(s) 1243
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 3 cut(s) 408, 1109, 1187
MslI CAYNNNNRTG 1 cut(s) 67
MspCI CTTAAG 1 cut(s) 1186
MspI CCGG 3 cut(s) 307, 767, 791
MspR9I CCNGG 1 cut(s) 767
MunI CAATTG 2 cut(s) 434, 530
MwoI GCNNNNNNNGC 5 cut(s) 89, 319, 367, 793, 1203
NciI CCSGG 1 cut(s) 767
NdeII GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
NlaIII CATG 7 cut(s) 66, 526, 655, 817, 907, 983, 1212
NlaIV GGNNCC 6 cut(s) 231, 369, 552, 795, 1269, 1327
NmuCI GTSAC 3 cut(s) 85, 696, 1198
NsbI TGCGCA 1 cut(s) 750
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 1 cut(s) 66
NspV TTCGAA 1 cut(s) 1151
PaeI GCATGC 1 cut(s) 66
PagI TCATGA 1 cut(s) 979
PceI AGGCCT 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 1148
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 8 cut(s) 204, 426, 574, 592, 838, 1018, 1153, 1259
PkrI GCNGC 2 cut(s) 721, 789
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
Psp124BI GAGCTC 1 cut(s) 363
PspN4I GGNNCC 6 cut(s) 231, 369, 552, 795, 1269, 1327
PspOMI GGGCCC 1 cut(s) 1267
PspPI GGNCC 2 cut(s) 1267, 1268
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 2 cut(s) 566, 810
RsaNI GTAC 2 cut(s) 565, 809
RseI CAYNNNNRTG 1 cut(s) 67
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 3 cut(s) 408, 1109, 1187
SatI GCNGC 2 cut(s) 720, 788
Sau3AI GATC 8 cut(s) 526, 550, 610, 711, 863, 900, 982, 1168
Sau96I GGNCC 2 cut(s) 1267, 1268
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 767
SduI GDGCHC 2 cut(s) 363, 1271
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 960
SfuI TTCGAA 1 cut(s) 1151
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 3 cut(s) 328, 1186, 1300
SmoI CTYRAG 3 cut(s) 328, 1186, 1300
SphI GCATGC 1 cut(s) 66
SseBI AGGCCT 1 cut(s) 1014
SsiI CCGC 3 cut(s) 291, 495, 683
SspMI CTAG 2 cut(s) 324, 854
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 1014
StyD4I CCNGG 1 cut(s) 765
StyI CCWWGG 1 cut(s) 1263
TaaI ACNGT 2 cut(s) 20, 1136
TaiI ACGT 2 cut(s) 9, 125
TaqI TCGA 8 cut(s) 381, 1026, 1047, 1092, 1142, 1151, 1171, 1345
TaqII GACCGA 1 cut(s) 179
TatI WGTACW 1 cut(s) 808
TfiI GAWTC 8 cut(s) 204, 426, 574, 592, 838, 1018, 1153, 1259
Tru1I TTAA 3 cut(s) 408, 1109, 1187
Tru9I TTAA 3 cut(s) 408, 1109, 1187
TseFI GTSAC 3 cut(s) 85, 696, 1198
TseI GCWGC 2 cut(s) 719, 787
Tsp45I GTSAC 3 cut(s) 85, 696, 1198
TspDTI ATGAA 5 cut(s) 154, 689, 693, 830, 968
TspGWI ACGGA 3 cut(s) 585, 715, 975
Vha464I CTTAAG 1 cut(s) 1186
XapI RAATTY 4 cut(s) 25, 52, 775, 879
XceI RCATGY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 2 cut(s) 324, 854
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.