FvH4_3g11981

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006563
Physical Location & Seq
Forward (+)
7086351 .. 7089515
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11981.t1

Sequence Viewer

Length: 1338 bp
ATGGACCTGTCTCCTCATTGCAAGCATTTCAGATCACTTGGTTGCAACTTGAATTCAATAGCATGCAAATGTGTGGTTTTAGTTATCATTGCTCTGGTTGTTAGAGCTGTCCTGCTTCCTACATACTCTAGTTTTAATGGGACTGAGCAGAACAAGTTGGTGTTGGTCAGCAACCTCTCCTTCTCAACTTTGTCAGGCAGTACTAATTTTGGTATCCGTAAAGATAAGTTTCTCGAGGTTCCTCAAATTGTATGGGGATTAAACAATCAGAAAATAGCATTTGCAAGAGCTTGTTTAACAGCAAGGCTTATGAACCGGATTCTGTTGATGCCTAGCTTGAGTGCTTCCTTGTTCTACAAAGAATTTGAGCTCTTGAAGCCCATTTCTTTTGATAAGGTCTTTAAGTTTGACAAGTTCAATTCACTCTGTGATGGCTATGTTCAGTTGGGGCAATACTCGGATCTTAAAAACCAAACAGGCGCGTTTGAGCTTCAGAAAGGAAGTGGAAGGAGGTGGACACCTGAGAGAGATTTGGATCAGTTGAAGCAGCACAGTAAGGGGCCATATGATGAACATGAGACGATTCGAATTGTTGGGAAGAATCCTTTTTTGTGGCATGATCATTGGCCTGTCAAGGACTATGCTAAGGTGTTTGAGTGCTTGGTTTTGGTTGATGAGATCATGAAAGAAGCCGATAAAGTTGTGTCGAGGATTAGAGAGATAGGAGCAGAACAAGTAAGAAGGAAAAGTGAGTCTGCTGAGAACTCTTTGTTGGTCGAGCCAGTGCCTTACGTAGCTGTTCATATGAGGATAGAATTAGATTGGATGATACACTGCAAGAACCTTGAGCGTAGATTGAACATAACTGAAATTTGTAGCAGCAAAGAAGAGATCATGGAGAGAGTTGGCAACATTGTTGGCCTTAAAACTCCAGTTGTTGTTTACTTAGCTGTGGCTGATAGTCTTCTACAAGATCCTTCAATTTTGAATGGATGGAAAGAAGGGTTGGTTCCTTTTGAGAAGAAGAAACTTGGTTTTGATGCACAATACAAGAAGTTCCCCTATCTCATTCAGTCTGCAATTGACTACGAAGTGTGCTTGAGAGCCGATGTGTTTGTTGGAAACAGTTTCTCTACATTTTCTAGCCTCATTGCTCTTGACAGAACCCAGAAGCTGATAAAAATGGGTGTCAATAGCTCATGTGGTGTTGATGTCAGGTGGCCTACATATGCATACAACATATTAGGGGAATCAAAAGGGCCTCAGAAATGGATGACAAACATGTCTGGCTCAAGTCTGAAAGCAATTAGCTATGGCTCCAACCAAATTTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

446

Amino Acids

50.44

Weight (kDa)

8.84

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 86 - 389 1.8e-22 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1282
AccII CGCG 1 cut(s) 482
AclWI GGATC 3 cut(s) 468, 543, 968
AcsI RAATTY 4 cut(s) 52, 362, 870, 1326
AcuI CTGAAG 1 cut(s) 476
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 1281
AgsI TTSAA 8 cut(s) 52, 57, 376, 418, 544, 859, 981, 988
AjuI GAANNNNNNNTTGG 4 cut(s) 755, 787, 989, 1021
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 15, 572
AlwI GGATC 3 cut(s) 468, 543, 968
AlwNI CAGNNNCTG 1 cut(s) 1174
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 5 cut(s) 560, 626, 919, 1220, 1259
ApeKI GCWGC 2 cut(s) 547, 879
ApoI RAATTY 4 cut(s) 52, 362, 870, 1326
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 3 cut(s) 4, 560, 1259
AsuII TTCGAA 1 cut(s) 586
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 956
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 559, 891
BccI CCATC 2 cut(s) 425, 987
BciVI GTATCC 1 cut(s) 224
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 15, 572
BfaI CTAG 3 cut(s) 129, 333, 1143
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 2 cut(s) 548, 880
BlsI GCNGC 2 cut(s) 549, 881
BmcAI AGTACT 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 3 cut(s) 4, 560, 1259
BmiI GGNNCC 4 cut(s) 240, 561, 1011, 1318
BmsI GCATC 2 cut(s) 318, 1030
BpiI GAAGAC 1 cut(s) 956
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 1 cut(s) 645
Bpu14I TTCGAA 1 cut(s) 586
BpuEI CTTGAG 4 cut(s) 358, 866, 1120, 1276
BsaAI YACGTR 1 cut(s) 793
BsaBI GATNNNNATC 1 cut(s) 534
BsaWI WCCGGW 1 cut(s) 315
Bse1I ACTGG 2 cut(s) 782, 932
Bse3DI GCAATG 3 cut(s) 16, 87, 1149
Bse8I GATNNNNATC 1 cut(s) 534
BseGI GGATG 3 cut(s) 831, 998, 1278
BseJI GATNNNNATC 1 cut(s) 534
BseMI GCAATG 3 cut(s) 16, 87, 1149
BseMII CTCAG 4 cut(s) 135, 513, 750, 1277
BseNI ACTGG 2 cut(s) 782, 932
BseXI GCAGC 2 cut(s) 559, 891
Bsh1236I CGCG 1 cut(s) 482
BshFI GGCC 5 cut(s) 562, 628, 921, 1222, 1261
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 1 cut(s) 316
BslFI GGGAC 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 15, 572
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 5 cut(s) 562, 628, 921, 1222, 1261
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
BspANI GGCC 5 cut(s) 562, 628, 921, 1222, 1261
BspCNI CTCAG 4 cut(s) 136, 514, 751, 1276
BspFNI CGCG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 4 cut(s) 240, 561, 1011, 1318
BspPI GGATC 3 cut(s) 468, 543, 968
BspT104I TTCGAA 1 cut(s) 586
BsrDI GCAATG 3 cut(s) 16, 87, 1149
BsrI ACTGG 2 cut(s) 782, 932
BssMI GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
Bst4CI ACNGT 2 cut(s) 554, 1127
Bst6I CTCTTC 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 23, 64
BstDEI CTNAG 6 cut(s) 144, 522, 645, 759, 946, 1263
BstF5I GGATG 3 cut(s) 831, 998, 1278
BstFNI CGCG 1 cut(s) 482
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 7 cut(s) 35, 463, 538, 622, 681, 894, 976
BstMAI GTCTC 2 cut(s) 15, 572
BstMBI GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNSI RCATGY 2 cut(s) 66, 1285
BstSNI TACGTA 1 cut(s) 793
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 2 cut(s) 559, 891
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 2 cut(s) 460, 973
BstYI RGATCY 2 cut(s) 460, 973
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 5 cut(s) 562, 628, 921, 1222, 1261
BtsCI GGATG 3 cut(s) 831, 998, 1278
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 2 cut(s) 789, 832
Cac8I GCNNGC 2 cut(s) 23, 64
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 1 cut(s) 681
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 3 cut(s) 4, 560, 1259
Csp6I GTAC 1 cut(s) 201
CviAII CATG 8 cut(s) 63, 575, 617, 682, 895, 1200, 1282, 1332
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 6 cut(s) 144, 522, 645, 759, 946, 1263
DpnI GATC 7 cut(s) 34, 462, 537, 621, 680, 893, 975
DpnII GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
DraIII CACNNNGTG 1 cut(s) 428
DrdI GACNNNNNNGTC 1 cut(s) 1282
DseDI GACNNNNNNGTC 1 cut(s) 1282
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Ecl136II GAGCTC 1 cut(s) 370
Eco105I TACGTA 1 cut(s) 793
Eco24I GRGCYC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 1259
EcoRI GAATTC 1 cut(s) 52
EcoT22I ATGCAT 1 cut(s) 1234
EcoT38I GRGCYC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 8 cut(s) 66, 578, 620, 685, 898, 1203, 1285, 1335
FaqI GGGAC 1 cut(s) 154
FatI CATG 8 cut(s) 62, 574, 616, 681, 894, 1199, 1281, 1331
FauNDI CATATG 3 cut(s) 565, 804, 1228
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 2 cut(s) 548, 880
FokI GGATG 3 cut(s) 838, 1005, 1285
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 548, 880
FspBI CTAG 3 cut(s) 129, 333, 1143
GlaI GCGC 1 cut(s) 481
GluI GCNGC 2 cut(s) 548, 880
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 5 cut(s) 562, 628, 921, 1222, 1261
HapII CCGG 1 cut(s) 316
HhaI GCGC 1 cut(s) 482
Hin1II CATG 8 cut(s) 66, 578, 620, 685, 898, 1203, 1285, 1335
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HinfI GANTC 5 cut(s) 319, 583, 601, 752, 1250
HpaII CCGG 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 516, 943
Hpy188I TCNGA 6 cut(s) 32, 270, 460, 495, 1266, 1299
Hpy188III TCNNGA 4 cut(s) 233, 373, 682, 1157
Hpy8I GTNNAC 2 cut(s) 516, 943
HpyAV CCTTC 5 cut(s) 190, 501, 735, 987, 995
HpyCH4III ACNGT 2 cut(s) 554, 1127
HpyCH4IV ACGT 1 cut(s) 792
HpyCH4V TGCA 8 cut(s) 21, 45, 66, 284, 837, 1043, 1079, 1232
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpyF3I CTNAG 6 cut(s) 144, 522, 645, 759, 946, 1263
HpySE526I ACGT 1 cut(s) 792
Hsp92II CATG 8 cut(s) 66, 578, 620, 685, 898, 1203, 1285, 1335
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
LmnI GCTCC 2 cut(s) 725, 1322
Lsp1109I GCAGC 2 cut(s) 559, 891
LweI GCATC 2 cut(s) 318, 1030
MaeI CTAG 3 cut(s) 129, 333, 1143
MaeII ACGT 1 cut(s) 792
MalI GATC 7 cut(s) 34, 462, 537, 621, 680, 893, 975
MboI GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
MboII GAAGA 5 cut(s) 610, 899, 956, 1033, 1036
MfeI CAATTG 1 cut(s) 1080
MflI RGATCY 2 cut(s) 460, 973
MhlI GDGCHC 1 cut(s) 372
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 1 cut(s) 1099
MnlI CCTC 9 cut(s) 24, 185, 229, 252, 504, 702, 801, 1157, 1272
Mph1103I ATGCAT 1 cut(s) 1234
MseI TTAA 6 cut(s) 135, 260, 296, 402, 465, 924
MslI CAYNNNNRTG 1 cut(s) 67
MspI CCGG 1 cut(s) 316
MunI CAATTG 1 cut(s) 1080
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 1 cut(s) 376
NdeI CATATG 3 cut(s) 565, 804, 1228
NdeII GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
NlaIII CATG 8 cut(s) 66, 578, 620, 685, 898, 1203, 1285, 1335
NlaIV GGNNCC 4 cut(s) 240, 561, 1011, 1318
NsiI ATGCAT 1 cut(s) 1234
NspI RCATGY 2 cut(s) 66, 1285
NspV TTCGAA 1 cut(s) 586
PaeI GCATGC 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 233
PagI TCATGA 1 cut(s) 681
PciI ACATGT 1 cut(s) 1281
PfeI GAWTC 4 cut(s) 319, 583, 601, 1250
PkrI GCNGC 2 cut(s) 549, 881
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
Ppu21I YACGTR 1 cut(s) 793
PscI ACATGT 1 cut(s) 1281
Psp124BI GAGCTC 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 240, 561, 1011, 1318
PspPI GGNCC 3 cut(s) 4, 560, 1259
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 2 cut(s) 460, 973
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 67
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 6 cut(s) 135, 260, 296, 402, 465, 924
SatI GCNGC 2 cut(s) 548, 880
Sau3AI GATC 7 cut(s) 32, 460, 535, 619, 678, 891, 973
Sau96I GGNCC 3 cut(s) 4, 560, 1259
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 1 cut(s) 761
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 2 cut(s) 318, 1030
Sfr274I CTCGAG 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 586
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 5 cut(s) 233, 337, 845, 1099, 1291
SmoI CTYRAG 5 cut(s) 233, 337, 845, 1099, 1291
SnaBI TACGTA 1 cut(s) 793
SphI GCATGC 1 cut(s) 66
SspMI CTAG 3 cut(s) 129, 333, 1143
SstI GAGCTC 1 cut(s) 372
TaaI ACNGT 2 cut(s) 554, 1127
TaiI ACGT 1 cut(s) 795
TaqI TCGA 4 cut(s) 234, 586, 707, 777
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 4 cut(s) 319, 583, 601, 1250
Tru1I TTAA 6 cut(s) 135, 260, 296, 402, 465, 924
Tru9I TTAA 6 cut(s) 135, 260, 296, 402, 465, 924
TscAI CASTG 2 cut(s) 789, 839
TseI GCWGC 2 cut(s) 547, 879
TspDTI ATGAA 5 cut(s) 326, 585, 698, 791, 1320
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 789, 839
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 4 cut(s) 52, 362, 870, 1326
XceI RCATGY 2 cut(s) 66, 1285
XhoI CTCGAG 1 cut(s) 233
XspI CTAG 3 cut(s) 129, 333, 1143
ZrmI AGTACT 1 cut(s) 202
Zsp2I ATGCAT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.