MD05G1256000.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006563
Physical Location & Seq
Reverse (-)
38669345 .. 38671795
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1256000.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGGACGTGCCTCCTTACTGTAAGAATTTCAGAGTTCTCGGTTGCCATTTGAATTCGTTCGCATGCAAATGCGTTGTTTTAGCTTTGACTGCTCTGATTATTAGAGCTGTTCTGCTTCCTACATTCTCCATCTCTGATGGAACTGAACAGGACAACTTGGTATTCATCAGTAATCTCTCGTTGTCATTCGGTCAGAAATCTGGAATCCGAAAAGATAAATTTTTGGAGGTTCCTCAAATCGTATGGGGATTAAACAATCAGAAAATAGCGTTCGCAAGAGCTTGTCTGACCGCAAAGTTTTTGAACCGGACGCTTTTGATGCCTAGCTTGAGTGCTTCCCTGTTTTACAAGGAAGTTGAGCTATTGGAACCGATTCCCTTTGATAAGGTCTTCCAGTTCAACAAGTTTAATTCTCTCTGCAATGGATTCGTTCAATTGGGTGAATTTACGGATGTTAGAAACCGAACAGCAGCATTTGAGCTTCAGAAAGGAAGTGGAAGGAGGTGGACTCCCGACAGGGACATGGATCAGTTGAAACACCATAATGAGGATCCATACAGTGAGTATGAGGTGATTCGAATTGTTGGAAGGAATCCGTTTCTGTGGCACGATCATTGGCCCGTGAAGGACTACGCGAAAATCTTTGAGTGCTTGGTTTTGGTTGATGAAATAATGAACGAAGCGGAGAAAGTTTTGTCAAGGATTAGAGAGATGGGAGCAGCACAAGTAAAAAGCCAGAGTGAGTCTGCGCAAAATGGCATTAGCCCGGCAGAGAATTCTCTCGTGCAGCCGGTGCCTTATGTGGCTGTACATATGCGGATAGAGATAGATTGGATGATTCACTGTAAGAAGCTGGAGCAAAGATCAAACATAACCCAAATTTGTAGTAGCAAGGAGGAGATCATGGAAAGAGTAGGCAACATTGTAGGCCTCAAAACGCCCGTCGTTGTTTATTTAGCTATAGCTGACAGTCTTCTTCATGATCCATCTATTCTGAACGGCTGGAAGTCGGGCTTGATTCCTTTCGAGAAGAAGAAATTGGGAGTTGAAGGGAATTACAAGAAGTTCCCTTATCTCATTCAGTCTGCAATCGACTACGAAGTGTGCTTGAGGGCTGATGTCTTCGTGGGAAACAGTTTCTCCACATTTTCAAATCTCATAGTTCTCGATCGAACGCAGAAGCTCATTAGAACGGGCGTCACAAGCTCATGTGGTGTGGATGTAAGGTGGCCTTCTTATGCATACAACATATTAGGGGAATCAAACGGACCTCAAAGATGGATGGCAAACATGTCTAGCTCAAGTCTTCAACCAATTAGCTATGGCTCCAACGACATCTCGTGTCAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

451

Amino Acids

50.9

Weight (kDa)

7.91

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 83 - 391 7.4e-24 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 750
AccB1I GGYRCC 1 cut(s) 793
AccII CGCG 1 cut(s) 635
AciI CCGC 3 cut(s) 291, 683, 817
AclWI GGATC 4 cut(s) 534, 545, 558, 977
AcsI RAATTY 6 cut(s) 25, 52, 218, 443, 775, 879
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 1197
AfaI GTAC 1 cut(s) 810
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 1290
AgsI TTSAA 8 cut(s) 52, 304, 400, 434, 535, 1049, 1152, 1310
AjiI CACGTC 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
AlwI GGATC 4 cut(s) 534, 545, 558, 977
AoxI GGCC 3 cut(s) 617, 928, 1229
ApeKI GCWGC 3 cut(s) 470, 719, 787
ApoI RAATTY 6 cut(s) 25, 52, 218, 443, 775, 879
ArsI GACNNNNNNTTYG 2 cut(s) 927, 959
Asp700I GAANNNNTTC 2 cut(s) 56, 372
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 2 cut(s) 618, 1268
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 452, 583
AsuII TTCGAA 1 cut(s) 577
AvaII GGWCC 1 cut(s) 1268
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 793
BauI CACGAG 2 cut(s) 782, 1339
BbsI GAAGAC 4 cut(s) 382, 965, 1114, 1298
BbvI GCAGC 3 cut(s) 482, 731, 799
BccI CCATC 6 cut(s) 131, 137, 706, 994, 1272, 1276
BceAI ACGGC 1 cut(s) 1015
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BcnI CCSGG 1 cut(s) 767
BfaI CTAG 2 cut(s) 324, 1296
BfmI CTRYAG 1 cut(s) 960
BisI GCNGC 3 cut(s) 471, 720, 788
BlsI GCNGC 3 cut(s) 472, 721, 789
Bme1390I CCNGG 1 cut(s) 767
Bme18I GGWCC 1 cut(s) 1268
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 618, 1268
BmiI GGNNCC 5 cut(s) 231, 369, 552, 795, 1327
BmrFI CCNGG 1 cut(s) 767
BmsI GCATC 1 cut(s) 309
BpiI GAAGAC 4 cut(s) 382, 965, 1114, 1298
BplI GAGNNNNNCTC 2 cut(s) 493, 525
BpmI CTGGAG 1 cut(s) 875
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 3 cut(s) 349, 1129, 1285
BpuMI CCSGG 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 1197
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 1124, 1154
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse118I RCCGGY 1 cut(s) 790
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 427
BseGI GGATG 4 cut(s) 457, 840, 1225, 1287
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 427
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 3 cut(s) 482, 731, 799
BsgI GTGCAG 1 cut(s) 806
Bsh1236I CGCG 1 cut(s) 635
Bsh1285I CGRYCG 1 cut(s) 1171
BshFI GGCC 3 cut(s) 619, 930, 1231
BshNI GGYRCC 1 cut(s) 793
BsiEI CGRYCG 1 cut(s) 1171
BsiSI CCGG 3 cut(s) 307, 767, 791
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 1 cut(s) 547
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 3 cut(s) 619, 930, 1231
Bsp119I TTCGAA 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
BspACI CCGC 3 cut(s) 291, 683, 817
BspANI GGCC 3 cut(s) 619, 930, 1231
BspFNI CGCG 1 cut(s) 635
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 5 cut(s) 231, 369, 552, 795, 1327
BspPI GGATC 4 cut(s) 534, 545, 558, 977
BspT104I TTCGAA 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 790
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 790
BssMI GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
BssNI GRCGYC 1 cut(s) 1197
BssSI CACGAG 2 cut(s) 782, 1339
Bst2BI CACGAG 2 cut(s) 782, 1339
Bst4CI ACNGT 5 cut(s) 20, 560, 845, 971, 1136
BstACI GRCGYC 1 cut(s) 1197
BstAPI GCANNNNNTGC 1 cut(s) 793
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 64
BstF5I GGATG 4 cut(s) 457, 840, 1225, 1287
BstFNI CGCG 1 cut(s) 635
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 7 cut(s) 529, 553, 613, 866, 903, 985, 1171
BstMBI GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
BstMCI CGRYCG 1 cut(s) 1171
BstMWI GCNNNNNNNGC 4 cut(s) 89, 319, 793, 1203
BstNSI RCATGY 2 cut(s) 66, 1294
BstSCI CCNGG 1 cut(s) 765
BstSFI CTRYAG 1 cut(s) 960
BstUI CGCG 1 cut(s) 635
BstV1I GCAGC 3 cut(s) 482, 731, 799
BstV2I GAAGAC 4 cut(s) 382, 965, 1114, 1298
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 3 cut(s) 619, 930, 1231
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 4 cut(s) 457, 840, 1225, 1287
BtsIMutI CAGTG 2 cut(s) 565, 841
Cac8I GCNNGC 1 cut(s) 64
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 790
Cfr13I GGNCC 2 cut(s) 618, 1268
CseI GACGC 2 cut(s) 319, 1186
Csp6I GTAC 1 cut(s) 809
CviAII CATG 6 cut(s) 63, 523, 904, 980, 1209, 1291
CviQI GTAC 1 cut(s) 809
DpnI GATC 7 cut(s) 528, 552, 612, 865, 902, 984, 1170
DpnII GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
Eco147I AGGCCT 1 cut(s) 930
Eco47I GGWCC 1 cut(s) 1268
Eco57I CTGAAG 1 cut(s) 467
EcoRI GAATTC 2 cut(s) 52, 775
EcoT22I ATGCAT 1 cut(s) 1243
FaeI CATG 6 cut(s) 66, 526, 907, 983, 1212, 1294
FalI AAGNNNNNCTT 4 cut(s) 998, 1030, 1216, 1248
FaqI GGGAC 1 cut(s) 533
FatI CATG 6 cut(s) 62, 522, 903, 979, 1208, 1290
FauNDI CATATG 1 cut(s) 813
Fnu4HI GCNGC 3 cut(s) 471, 720, 788
FokI GGATG 4 cut(s) 464, 847, 1232, 1294
Fsp4HI GCNGC 3 cut(s) 471, 720, 788
FspBI CTAG 2 cut(s) 324, 1296
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 1 cut(s) 750
GluI GCNGC 3 cut(s) 471, 720, 788
GsuI CTGGAG 1 cut(s) 875
HaeIII GGCC 3 cut(s) 619, 930, 1231
HapII CCGG 3 cut(s) 307, 767, 791
HgaI GACGC 2 cut(s) 319, 1186
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 1 cut(s) 1197
Hin1II CATG 6 cut(s) 66, 526, 907, 983, 1212, 1294
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HpaII CCGG 3 cut(s) 307, 767, 791
HphI GGTGA 2 cut(s) 452, 583
Hpy166II GTNNAC 1 cut(s) 507
Hpy188III TCNNGA 5 cut(s) 201, 512, 980, 1027, 1166
Hpy8I GTNNAC 1 cut(s) 507
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 5 cut(s) 492, 582, 619, 1043, 1242
HpyCH4III ACNGT 5 cut(s) 20, 560, 845, 971, 1136
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 5 cut(s) 66, 420, 787, 1088, 1241
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 319, 793, 1203
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 1197
Hsp92II CATG 6 cut(s) 66, 526, 907, 983, 1212, 1294
HspAI GCGC 1 cut(s) 749
Kzo9I GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
LmnI GCTCC 3 cut(s) 716, 856, 1331
Lsp1109I GCAGC 3 cut(s) 482, 731, 799
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 324, 1296
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 1198
MalI GATC 7 cut(s) 528, 552, 612, 865, 902, 984, 1170
MboI GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
MboII GAAGA 7 cut(s) 382, 965, 968, 1042, 1045, 1114, 1298
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 1 cut(s) 550
MlyI GAGTC 2 cut(s) 502, 752
MmeI TCCRAC 2 cut(s) 565, 1353
Mph1103I ATGCAT 1 cut(s) 1243
MroXI GAANNNNTTC 2 cut(s) 56, 372
MseI TTAA 2 cut(s) 251, 408
MslI CAYNNNNRTG 2 cut(s) 67, 543
MspI CCGG 3 cut(s) 307, 767, 791
MspR9I CCNGG 1 cut(s) 767
MunI CAATTG 1 cut(s) 434
MvnI CGCG 1 cut(s) 635
MwoI GCNNNNNNNGC 4 cut(s) 89, 319, 793, 1203
NciI CCSGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 813
NdeII GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
NlaIII CATG 6 cut(s) 66, 526, 907, 983, 1212, 1294
NlaIV GGNNCC 5 cut(s) 231, 369, 552, 795, 1327
NmuCI GTSAC 1 cut(s) 1198
NsbI TGCGCA 1 cut(s) 750
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 2 cut(s) 66, 1294
NspV TTCGAA 1 cut(s) 577
PaeI GCATGC 1 cut(s) 66
PagI TCATGA 1 cut(s) 979
PceI AGGCCT 1 cut(s) 930
PciI ACATGT 1 cut(s) 1290
PdmI GAANNNNTTC 2 cut(s) 56, 372
PfeI GAWTC 8 cut(s) 204, 373, 426, 574, 592, 838, 1018, 1259
PkrI GCNGC 3 cut(s) 472, 721, 789
Ple19I CGATCG 1 cut(s) 1171
PleI GAGTC 2 cut(s) 502, 751
PpsI GAGTC 2 cut(s) 502, 751
PscI ACATGT 1 cut(s) 1290
PspN4I GGNNCC 5 cut(s) 231, 369, 552, 795, 1327
PspPI GGNCC 2 cut(s) 618, 1268
PsuI RGATCY 1 cut(s) 550
PvuI CGATCG 1 cut(s) 1171
RsaI GTAC 1 cut(s) 810
RsaNI GTAC 1 cut(s) 809
RseI CAYNNNNRTG 2 cut(s) 67, 543
SaqAI TTAA 2 cut(s) 251, 408
SatI GCNGC 3 cut(s) 471, 720, 788
Sau3AI GATC 7 cut(s) 526, 550, 610, 863, 900, 982, 1168
Sau96I GGNCC 2 cut(s) 618, 1268
SchI GAGTC 2 cut(s) 502, 752
ScrFI CCNGG 1 cut(s) 767
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 960
SfuI TTCGAA 1 cut(s) 577
SinI GGWCC 1 cut(s) 1268
SmiMI CAYNNNNRTG 2 cut(s) 67, 543
SmlI CTYRAG 3 cut(s) 328, 1108, 1300
SmoI CTYRAG 3 cut(s) 328, 1108, 1300
SphI GCATGC 1 cut(s) 66
SseBI AGGCCT 1 cut(s) 930
SsiI CCGC 3 cut(s) 291, 683, 817
SspMI CTAG 2 cut(s) 324, 1296
StuI AGGCCT 1 cut(s) 930
StyD4I CCNGG 1 cut(s) 765
TaaI ACNGT 5 cut(s) 20, 560, 845, 971, 1136
TaiI ACGT 1 cut(s) 9
TaqI TCGA 5 cut(s) 577, 1026, 1092, 1167, 1171
TaqII GACCGA 1 cut(s) 179
TatI WGTACW 1 cut(s) 808
TfiI GAWTC 8 cut(s) 204, 373, 426, 574, 592, 838, 1018, 1259
Tru1I TTAA 2 cut(s) 251, 408
Tru9I TTAA 2 cut(s) 251, 408
TscAI CASTG 2 cut(s) 565, 848
TseFI GTSAC 1 cut(s) 1198
TseI GCWGC 3 cut(s) 470, 719, 787
Tsp45I GTSAC 1 cut(s) 1198
TspDTI ATGAA 4 cut(s) 154, 681, 689, 968
TspGWI ACGGA 3 cut(s) 464, 585, 1281
TspRI CASTG 2 cut(s) 565, 848
VpaK11BI GGWCC 1 cut(s) 1268
XapI RAATTY 6 cut(s) 25, 52, 218, 443, 775, 879
XceI RCATGY 2 cut(s) 66, 1294
XmnI GAANNNNTTC 2 cut(s) 56, 372
XspI CTAG 2 cut(s) 324, 1296
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.