Rh5BG141500

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006563
Physical Location & Seq
Forward (+)
14651678 .. 14652103
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG141500.1

Sequence Viewer

Length: 426 bp
ATGAAGATAGAGTTAGATTGGATGATACACTGTAAGAATCTCGAGCGTAGATTGAACATAACCGATATTTGTAGCAGCAAGGGGGAGATCATGGAGAGAGTTGGCAACATTGTTGGCCTCAAGACGCCAGCTGTTGTTTATTTAGCTGTGGCTGATAGTCTTCTTCAAGATCCATCAATTTTGAATGGATGGAAAGAAGGGTTGGTTCCATTTGAGAAGAAGAAACTGGGTTTTGATGCACGTTACAAGAAGTTCCCCTATCTCATTCAGTCTGCGATTGACTATGAAGTGTGCTTGAGAGCCGATGTGTTTGTTGGAAACAGTTTCTCTACATTTTCTAGCTTCCTTGCTCTTGACAGAACCCAGAAGCTGATCAAAATGGGAGTCAATAGCTCATGTGGTGTAGATGTGAGGTGGCCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

141

Amino Acids

15.95

Weight (kDa)

8.68

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 72 - 121 1.6e-06 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 125
AgsI TTSAA 3 cut(s) 55, 167, 184
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 5 cut(s) 131, 146, 342, 370, 393
AluI AGCT 5 cut(s) 131, 146, 342, 370, 393
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 2 cut(s) 115, 416
ApeKI GCWGC 1 cut(s) 75
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AvaI CYCGRG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 87
BccI CCATC 2 cut(s) 181, 183
BclI TGATCA 1 cut(s) 372
BfaI CTAG 1 cut(s) 339
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
BmeT110I CYCGRG 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 226
BmuI ACTGGG 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 152
BpuEI CTTGAG 2 cut(s) 104, 316
BsaHI GRCGYC 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 27, 194
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 87
BshFI GGCC 2 cut(s) 117, 418
BsiHKCI CYCGRG 1 cut(s) 41
BsnI GGCC 2 cut(s) 117, 418
BsoBI CYCGRG 1 cut(s) 41
Bsp143I GATC 3 cut(s) 87, 169, 372
BspANI GGCC 2 cut(s) 117, 418
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 231
BssMI GATC 3 cut(s) 87, 169, 372
BssNI GRCGYC 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 32, 323
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 27, 194
BstKTI GATC 3 cut(s) 90, 172, 375
BstMBI GATC 3 cut(s) 87, 169, 372
BstV1I GCAGC 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 2 cut(s) 117, 418
BtsCI GGATG 2 cut(s) 27, 194
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 370
CseI GACGC 1 cut(s) 133
CviAII CATG 2 cut(s) 91, 396
CviJI RGCY 9 cut(s) 117, 131, 146, 152, 302, 342, 370, 393, 418
CviKI_1 RGCY 9 cut(s) 117, 131, 146, 152, 302, 342, 370, 393, 418
DdeI CTNAG 1 cut(s) 423
DpnI GATC 3 cut(s) 89, 171, 374
DpnII GATC 3 cut(s) 87, 169, 372
Eco88I CYCGRG 1 cut(s) 41
FaeI CATG 2 cut(s) 94, 399
FaiI YATR 4 cut(s) 59, 92, 285, 397
FatI CATG 2 cut(s) 90, 395
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 2 cut(s) 34, 201
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 1 cut(s) 339
GluI GCNGC 1 cut(s) 76
HaeIII GGCC 2 cut(s) 117, 418
HgaI GACGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 2 cut(s) 94, 399
HinfI GANTC 2 cut(s) 37, 384
Hpy188III TCNNGA 4 cut(s) 41, 121, 167, 353
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 32, 323
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 241
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 2 cut(s) 94, 399
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 3 cut(s) 87, 169, 372
LpnPI CCDG 3 cut(s) 141, 212, 377
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 242
MalI GATC 3 cut(s) 89, 171, 374
MboI GATC 3 cut(s) 87, 169, 372
MboII GAAGA 5 cut(s) 16, 152, 155, 229, 232
MflI RGATCY 1 cut(s) 169
MluCI AATT 1 cut(s) 177
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 2 cut(s) 128, 405
MspA1I CMGCKG 1 cut(s) 131
NdeII GATC 3 cut(s) 87, 169, 372
NlaIII CATG 2 cut(s) 94, 399
NlaIV GGNNCC 1 cut(s) 207
PaeR7I CTCGAG 1 cut(s) 41
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 131
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 3 cut(s) 87, 169, 372
SchI GAGTC 1 cut(s) 393
SetI ASST 7 cut(s) 133, 148, 244, 344, 372, 395, 416
SfaNI GCATC 1 cut(s) 226
Sfr274I CTCGAG 1 cut(s) 41
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 3 cut(s) 41, 119, 295
SmoI CTYRAG 3 cut(s) 41, 119, 295
Sse9I AATT 1 cut(s) 177
SspMI CTAG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 32, 323
TaiI ACGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 177
TfiI GAWTC 1 cut(s) 37
TscAI CASTG 1 cut(s) 35
TseI GCWGC 1 cut(s) 75
TspDTI ATGAA 2 cut(s) 17, 300
TspRI CASTG 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 41
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.