AT3G06520

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008214
Physical Location & Seq
Forward (+)
2019849 .. 2022102
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06520.1

Sequence Viewer

Length: 1401 bp
ATGACGAGCGATCGATTTTGGCGCGGCGGTGACCGCGTTGAGGTGGAACGACTAGTTTCTGGCGCGACGGCATATTTTCCGGCGAGTGTTGTTAGCGCGCCTTCGGTGAGAAAGAAACTCGTTTGGGTGGAGCATGAATCCCTAACTGTTGGAGGTTCCGTTTCCGTACGTATGAAGGAGTACGTCACTCCCACGCGCCTCCGTCCATCTCCACCGCGTGAACTCAACAGACGGTTCAAAGCTGACGATGAGGTCGATGTCTTCAGAGATAGCGAAGGTTGCTGGGTTAGAGGCAATGTTACGACCGTACTTGAAGATTCAAGGTACATTGTGGAGTTTAAAGGGGAAAATCGTCCAGAAATAGAGGTTGATCAGTTCAATTTGAGGCTTCATCGTGAATGGCTAGATGGTGGGTGGGTTCCTTCTCTTCTCCAACAGAGCAATTTCTCGGAATCGACAGCCCAAAGAATTAAACTGAAGATAAAAATCAAGAGAAGAGATCAATATGAGAAGGGAGCTCTTGTCGAAGTCAGAAGTGAAGAAAAAGCTTACAAGGGTTCTTGGTATTGTGCTCGTATACTTTGTTTATTAGGTGATGATAAGTACATTGTTGAACACTTGAAATTTAGTAGAGATGATGGTGAGTCGATCCCTCTGAGAGATGTGGTAGAAGCCAAGGACATAAGACCAGTTCCTCCTTCAGAACTCTCGCCTGTTGTGTGCTATGAACCAGGGGTTATAGTTGATGCTTGGTTCAACAAACGGTGGTGGACCAGTAGGGTTTCCAAAGTCCTAGGAGGAGGTTCTAATAAGTATTCAGTTTTTATCATCTCAACTGGTGAAGAAACGACAATCTTAAATTTCAACTTAAGGCCCCACAAAGATTGGATCAATGGACAATGGGTCATTCCTTCCAAGGTATTGACTGATGTGCCGGAAGAATGTTACAAACCTCCATTGAAGAAGCTGAAATCTTGTGAGAGAGCAGAGAAGGTGTTTAACAATGGAATGGAGGTGGAAGTTAGAAGCGACGAACCAGGTTACGAAGCCTCATGGTTCTCTGCAAAGATCGTAAGTTACTTGGGCGAAAACAGATACACAGTGGAGTATCAAACACTAAAAACAGACGATGAAAGAGAGTTGTTGAAGGAAGAAGCAAGAGGTTCAGATATAAGACCTCCTCCTCCTCCACTGATTCCAAAGGGGTATCGATATGAATTGTATGAACTTGTGGATGCTTGGTACAATGAAGGATGGTGGTCTGGTCGAGTATATAAGATAAACAACAACAAAACGAGATATGGTGTCTACTTCCAAACGACTGATGAGAGTTTGGAGTTTGCTTACAATGACCTTAGGCCTTGCCAAGTGTGGAGAAACGGAAAATGGAGTCGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

466

Amino Acids

54.22

Weight (kDa)

7.51

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 10 - 72 5.4e-07 Agenet domain
Agenet PF05641 82 - 131 7.4e-06 Agenet domain
Agenet PF05641 172 - 233 5e-16 Agenet domain
Agenet PF05641 336 - 396 4.6e-19 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccI GTMKAC 2 cut(s) 577, 1308
AccII CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
AciI CCGC 4 cut(s) 24, 27, 34, 215
AclWI GGATC 2 cut(s) 643, 896
AcsI RAATTY 2 cut(s) 623, 859
AcuI CTGAAG 3 cut(s) 247, 497, 684
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 6 cut(s) 168, 182, 309, 326, 605, 1244
AfiI CCNNNNNNNGG 1 cut(s) 40
AflII CTTAAG 1 cut(s) 868
AhdI GACNNNNNGTC 1 cut(s) 902
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 2 cut(s) 730, 1036
AluBI AGCT 4 cut(s) 242, 518, 548, 967
AluI AGCT 4 cut(s) 242, 518, 548, 967
Alw21I GWGCWC 2 cut(s) 520, 574
AlwI GGATC 2 cut(s) 643, 896
AoxI GGCC 2 cut(s) 872, 1358
ApoI RAATTY 2 cut(s) 623, 859
AspA2I CCTAGG 1 cut(s) 793
AspLEI GCGC 5 cut(s) 24, 65, 98, 100, 198
AspS9I GGNCC 2 cut(s) 771, 873
AsuHPI GGTGA 5 cut(s) 41, 118, 605, 653, 851
AvaII GGWCC 1 cut(s) 771
AvrII CCTAGG 1 cut(s) 793
AxyI CCTNAGG 1 cut(s) 1355
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 520, 574
BccI CCATC 4 cut(s) 214, 401, 632, 1248
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 2 cut(s) 732, 1038
BclI TGATCA 1 cut(s) 370
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 3 cut(s) 53, 404, 794
BfrI CTTAAG 1 cut(s) 868
BisI GCNGC 1 cut(s) 25
BlnI CCTAGG 1 cut(s) 793
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 732, 1038
Bme18I GGWCC 1 cut(s) 771
BmeRI GACNNNNNGTC 1 cut(s) 902
BmgT120I GGNCC 2 cut(s) 771, 873
BmiI GGNNCC 3 cut(s) 157, 420, 875
BmrFI CCNGG 2 cut(s) 732, 1038
BmsI GCATC 2 cut(s) 736, 1225
BpiI GAAGAC 1 cut(s) 253
Bsa29I ATCGAT 2 cut(s) 13, 1210
BsaAI YACGTR 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 675, 731, 793, 915
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 689, 774, 841
Bse21I CCTNAGG 1 cut(s) 1355
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 2 cut(s) 732, 1038
BseCI ATCGAT 2 cut(s) 13, 1210
BseDI CCNNGG 4 cut(s) 675, 731, 793, 915
BseGI GGATG 2 cut(s) 1240, 1259
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 3 cut(s) 689, 774, 841
BsePI GCGCGC 1 cut(s) 96
BseRI GAGGAG 4 cut(s) 813, 1170, 1173, 1176
BseYI CCCAGC 1 cut(s) 282
Bsh1236I CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
Bsh1285I CGRYCG 2 cut(s) 13, 306
BshFI GGCC 2 cut(s) 874, 1360
BshVI ATCGAT 2 cut(s) 13, 1210
BsiEI CGRYCG 2 cut(s) 13, 306
BsiHKAI GWGCWC 2 cut(s) 520, 574
BsiSI CCGG 2 cut(s) 80, 935
BsiWI CGTACG 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 40
BsnI GGCC 2 cut(s) 874, 1360
Bsp1286I GDGCHC 2 cut(s) 520, 574
Bsp143I GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
BspACI CCGC 4 cut(s) 24, 27, 34, 215
BspANI GGCC 2 cut(s) 874, 1360
BspCNI CTCAG 1 cut(s) 648
BspDI ATCGAT 2 cut(s) 13, 1210
BspFNI CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
BspLI GGNNCC 3 cut(s) 157, 420, 875
BspPI GGATC 2 cut(s) 643, 896
BspTI CTTAAG 1 cut(s) 868
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 3 cut(s) 689, 774, 841
BssECI CCNNGG 4 cut(s) 675, 731, 793, 915
BssHII GCGCGC 1 cut(s) 96
BssMI GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
BssNAI GTATAC 1 cut(s) 578
BssT1I CCWWGG 3 cut(s) 675, 793, 915
Bst1107I GTATAC 1 cut(s) 578
Bst2UI CCWGG 2 cut(s) 732, 1038
Bst4CI ACNGT 5 cut(s) 148, 234, 307, 765, 1102
Bst6I CTCTTC 2 cut(s) 432, 490
BstAFI CTTAAG 1 cut(s) 868
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 656, 1355
BstEII GGTNACC 1 cut(s) 29
BstF5I GGATG 2 cut(s) 1240, 1259
BstFNI CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
BstHHI GCGC 5 cut(s) 24, 65, 98, 100, 198
BstKTI GATC 6 cut(s) 13, 373, 502, 651, 891, 1071
BstMBI GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
BstMCI CGRYCG 2 cut(s) 13, 306
BstMWI GCNNNNNNNGC 2 cut(s) 33, 279
BstNI CCWGG 2 cut(s) 732, 1038
BstPI GGTNACC 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 730, 1036
BstSNI TACGTA 1 cut(s) 170
BstUI CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
BstV2I GAAGAC 1 cut(s) 253
BstZ17I GTATAC 1 cut(s) 578
Bsu15I ATCGAT 2 cut(s) 13, 1210
Bsu36I CCTNAGG 1 cut(s) 1355
BsuRI GGCC 2 cut(s) 874, 1360
BsuTUI ATCGAT 2 cut(s) 13, 1210
BtsCI GGATG 2 cut(s) 1240, 1259
BtsIMutI CAGTG 2 cut(s) 1107, 1190
Cac8I GCNNGC 1 cut(s) 98
CfoI GCGC 5 cut(s) 24, 65, 98, 100, 198
Cfr13I GGNCC 2 cut(s) 771, 873
ClaI ATCGAT 2 cut(s) 13, 1210
CsiI ACCWGGT 1 cut(s) 1036
Csp6I GTAC 6 cut(s) 167, 181, 308, 325, 604, 1243
CspCI CAANNNNNGTGG 2 cut(s) 866, 901
CviAII CATG 2 cut(s) 134, 1053
CviQI GTAC 6 cut(s) 167, 181, 308, 325, 604, 1243
DdeI CTNAG 2 cut(s) 656, 1355
DpnI GATC 6 cut(s) 12, 372, 501, 650, 890, 1070
DpnII GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
DraI TTTAAA 1 cut(s) 340
DraIII CACNNNGTG 1 cut(s) 218
DrdI GACNNNNNNGTC 1 cut(s) 251
DriI GACNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 432, 490
Eam1105I GACNNNNNGTC 1 cut(s) 902
EarI CTCTTC 2 cut(s) 432, 490
Ecl136II GAGCTC 1 cut(s) 518
Eco105I TACGTA 1 cut(s) 170
Eco130I CCWWGG 3 cut(s) 675, 793, 915
Eco147I AGGCCT 1 cut(s) 1360
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 518
Eco57I CTGAAG 3 cut(s) 247, 497, 684
Eco81I CCTNAGG 1 cut(s) 1355
Eco91I GGTNACC 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 873
EcoO65I GGTNACC 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 730, 1036
EcoT14I CCWWGG 3 cut(s) 675, 793, 915
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 3 cut(s) 675, 793, 915
FaeI CATG 2 cut(s) 137, 1056
FatI CATG 2 cut(s) 133, 1052
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 2 cut(s) 577, 1308
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 1247, 1266
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 3 cut(s) 53, 404, 794
GlaI GCGC 5 cut(s) 23, 64, 97, 99, 197
GluI GCNGC 1 cut(s) 25
GsaI CCCAGC 1 cut(s) 286
HaeIII GGCC 2 cut(s) 874, 1360
HapII CCGG 2 cut(s) 80, 935
HhaI GCGC 5 cut(s) 24, 65, 98, 100, 198
Hin1II CATG 2 cut(s) 137, 1056
Hin6I GCGC 5 cut(s) 22, 63, 96, 98, 196
HinP1I GCGC 5 cut(s) 22, 63, 96, 98, 196
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 6 cut(s) 137, 317, 452, 644, 1195, 1390
HpaII CCGG 2 cut(s) 80, 935
HphI GGTGA 5 cut(s) 41, 118, 605, 653, 851
Hpy166II GTNNAC 4 cut(s) 221, 578, 771, 1309
Hpy188I TCNGA 6 cut(s) 266, 451, 533, 657, 703, 1168
Hpy188III TCNNGA 3 cut(s) 356, 395, 490
Hpy8I GTNNAC 4 cut(s) 221, 578, 771, 1309
Hpy99I CGWCG 2 cut(s) 70, 1034
HpyCH4III ACNGT 5 cut(s) 148, 234, 307, 765, 1102
HpyCH4IV ACGT 2 cut(s) 169, 183
HpyCH4V TGCA 1 cut(s) 1064
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 279
HpyF3I CTNAG 2 cut(s) 656, 1355
HpySE526I ACGT 2 cut(s) 169, 183
Hsp92II CATG 2 cut(s) 137, 1056
HspAI GCGC 5 cut(s) 22, 63, 96, 98, 196
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
LmnI GCTCC 2 cut(s) 130, 515
LweI GCATC 2 cut(s) 736, 1225
MabI ACCWGGT 1 cut(s) 1036
MaeI CTAG 3 cut(s) 53, 404, 794
MaeII ACGT 2 cut(s) 169, 183
MaeIII GTNAC 6 cut(s) 29, 184, 298, 944, 1040, 1076
MalI GATC 6 cut(s) 12, 372, 501, 650, 890, 1070
MboI GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
MhlI GDGCHC 2 cut(s) 520, 574
MluCI AATT 6 cut(s) 379, 442, 468, 623, 859, 1217
MlyI GAGTC 2 cut(s) 653, 1399
MmeI TCCRAC 2 cut(s) 130, 457
MseI TTAA 5 cut(s) 339, 471, 857, 869, 999
MspCI CTTAAG 1 cut(s) 868
MspI CCGG 2 cut(s) 80, 935
MspR9I CCNGG 2 cut(s) 732, 1038
MvaI CCWGG 2 cut(s) 732, 1038
MvnI CGCG 6 cut(s) 24, 36, 65, 98, 196, 217
MwoI GCNNNNNNNGC 2 cut(s) 33, 279
NdeII GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
NlaIII CATG 2 cut(s) 137, 1056
NlaIV GGNNCC 3 cut(s) 157, 420, 875
NmuCI GTSAC 2 cut(s) 29, 184
PauI GCGCGC 1 cut(s) 96
PceI AGGCCT 1 cut(s) 1360
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 4 cut(s) 137, 317, 452, 1195
Pfl23II CGTACG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 26
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 2 cut(s) 652, 1398
PpsI GAGTC 2 cut(s) 652, 1398
Ppu21I YACGTR 1 cut(s) 170
Psp124BI GAGCTC 1 cut(s) 520
Psp6I CCWGG 2 cut(s) 730, 1036
PspEI GGTNACC 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 2 cut(s) 730, 1036
PspLI CGTACG 1 cut(s) 166
PspN4I GGNNCC 3 cut(s) 157, 420, 875
PspPI GGNCC 2 cut(s) 771, 873
PteI GCGCGC 1 cut(s) 96
PvuI CGATCG 1 cut(s) 13
RsaI GTAC 6 cut(s) 168, 182, 309, 326, 605, 1244
RsaNI GTAC 6 cut(s) 167, 181, 308, 325, 604, 1243
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 5 cut(s) 339, 471, 857, 869, 999
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 6 cut(s) 10, 370, 499, 648, 888, 1068
Sau96I GGNCC 2 cut(s) 771, 873
SchI GAGTC 2 cut(s) 653, 1399
ScrFI CCNGG 2 cut(s) 732, 1038
SduI GDGCHC 2 cut(s) 520, 574
SexAI ACCWGGT 1 cut(s) 1036
SfaNI GCATC 2 cut(s) 736, 1225
SinI GGWCC 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
SnaBI TACGTA 1 cut(s) 170
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 6 cut(s) 379, 442, 468, 623, 859, 1217
SseBI AGGCCT 1 cut(s) 1360
SsiI CCGC 4 cut(s) 24, 27, 34, 215
SspMI CTAG 3 cut(s) 53, 404, 794
SstI GAGCTC 1 cut(s) 520
StuI AGGCCT 1 cut(s) 1360
StyD4I CCNGG 2 cut(s) 730, 1036
StyI CCWWGG 3 cut(s) 675, 793, 915
TaaI ACNGT 5 cut(s) 148, 234, 307, 765, 1102
TaiI ACGT 2 cut(s) 172, 186
TaqI TCGA 7 cut(s) 13, 255, 455, 525, 647, 1210, 1267
TasI AATT 6 cut(s) 379, 442, 468, 623, 859, 1217
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 4 cut(s) 137, 317, 452, 1195
Tru1I TTAA 5 cut(s) 339, 471, 857, 869, 999
Tru9I TTAA 5 cut(s) 339, 471, 857, 869, 999
TscAI CASTG 2 cut(s) 1107, 1197
TseFI GTSAC 2 cut(s) 29, 184
Tsp45I GTSAC 2 cut(s) 29, 184
TspDTI ATGAA 8 cut(s) 150, 188, 380, 741, 1146, 1230, 1239, 1263
TspGWI ACGGA 4 cut(s) 148, 154, 191, 1395
TspRI CASTG 2 cut(s) 1107, 1197
Vha464I CTTAAG 1 cut(s) 868
VpaK11BI GGWCC 1 cut(s) 771
XapI RAATTY 2 cut(s) 623, 859
XmaJI CCTAGG 1 cut(s) 793
XmiI GTMKAC 2 cut(s) 577, 1308
XspI CTAG 3 cut(s) 53, 404, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.