Rh3BG084000

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008214
Physical Location & Seq
Reverse (-)
6596160 .. 6597979
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG084000.1

Sequence Viewer

Length: 645 bp
ATGCCTCGAAAGCCTCGAAGAACCAAAGTCTTCAACTTCAGTAAATGCCTGACTCCTGGTTTGGTCCCCATGCCGGAAACGCCTCCGCCGTCTCCGCCGCGTGAAGAATTGGTAGAAGAAGTTGGAGAGAATGACCCTAATGTAACTGATTCAATTGTGAGCAATGAAATCTTCACCAAGGGAGCACTGGTTGAGGTTAGCAGTGATGAAGAAGGATTCCATGGTTCTTGGTTTGCTGCCACTGTAGTGAAATCTGTGGGGAAAGACAAGTTCCTTATTGAGTACAAGTGCTTGAGGACAGAGGATGATTTGGCATTTTTGAGAGAAGAGGTTGATAACATGCACATACGACCTCATCCACCAGAAAATGTTGTTGATTGTTTCAAGCAACTTGACGAGGTTGATGCTTTGTACAATGATGGGTGGTGGGTTGGTGTTATTTCTAAGGTTCTAAGAGGTTCAAGGTACATAGTCTACTTCAAGGAAACCTGTGAGGAACTTGAATATCAGCACTCTGAGTTAAGGCTTCATCAGGACTGGATTGATGGCAAATGGGTTATCCCATCTCAGGTTGCCATAAATGGAAAGATGCAGGACAGTTATAATGCATTGATTGTTAGAACTGCTTACAGTTCCACCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

214

Amino Acids

24.54

Weight (kDa)

4.97

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 61 - 121 2.5e-20 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccI GTMKAC 1 cut(s) 474
AccII CGCG 1 cut(s) 100
AciI CCGC 3 cut(s) 86, 95, 98
AcuI CTGAAG 1 cut(s) 22
AfaI GTAC 3 cut(s) 284, 413, 467
AfiI CCNNNNNNNGG 2 cut(s) 73, 568
AgsI TTSAA 6 cut(s) 34, 153, 385, 462, 481, 503
AjnI CCWGG 1 cut(s) 55
AleI CACNNNNGTG 1 cut(s) 245
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 236
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 166
AvaII GGWCC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 223
BccI CCATC 3 cut(s) 413, 539, 571
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 1 cut(s) 643
BfmI CTRYAG 1 cut(s) 243
BisI GCNGC 2 cut(s) 98, 237
BlsI GCNGC 2 cut(s) 99, 238
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 2 cut(s) 394, 579
BpiI GAAGAC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 313
BsaJI CCNNGG 2 cut(s) 177, 220
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 568
Bse1I ACTGG 2 cut(s) 192, 542
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 177, 220
BseGI GGATG 2 cut(s) 310, 355
BseLI CCNNNNNNNGG 2 cut(s) 73, 568
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 507, 581
BseNI ACTGG 2 cut(s) 192, 542
BseXI GCAGC 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 73, 568
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 187
Bsp1407I TGTACA 1 cut(s) 411
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 3 cut(s) 86, 95, 98
BspCNI CTCAG 2 cut(s) 508, 580
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 2 cut(s) 192, 542
BssECI CCNNGG 2 cut(s) 177, 220
BssT1I CCWWGG 2 cut(s) 177, 220
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 244, 599, 632
Bst6I CTCTTC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 411
BstDEI CTNAG 4 cut(s) 444, 452, 516, 567
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 2 cut(s) 310, 355
BstFNI CGCG 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 96
BstMWI GCNNNNNNNGC 3 cut(s) 10, 79, 94
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 243
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 310, 355
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 3 cut(s) 185, 208, 240
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 3 cut(s) 283, 412, 466
CviAII CATG 3 cut(s) 70, 221, 340
CviJI RGCY 2 cut(s) 13, 526
CviKI_1 RGCY 2 cut(s) 13, 526
CviQI GTAC 3 cut(s) 283, 412, 466
DdeI CTNAG 4 cut(s) 444, 452, 516, 567
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 2 cut(s) 75, 84
Eco130I CCWWGG 2 cut(s) 177, 220
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 177, 220
EcoT22I ATGCAT 1 cut(s) 610
ErhI CCWWGG 2 cut(s) 177, 220
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 3 cut(s) 73, 224, 343
FaiI YATR 7 cut(s) 71, 222, 341, 347, 470, 578, 603
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 69, 220, 339
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 2 cut(s) 98, 237
FokI GGATG 2 cut(s) 317, 342
Fsp4HI GCNGC 2 cut(s) 98, 237
FspBI CTAG 1 cut(s) 643
GluI GCNGC 2 cut(s) 98, 237
HapII CCGG 1 cut(s) 74
Hin1II CATG 3 cut(s) 73, 224, 343
HinfI GANTC 3 cut(s) 52, 149, 216
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 1 cut(s) 517
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 3 cut(s) 244, 599, 632
HpyCH4V TGCA 3 cut(s) 343, 592, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 79, 94
HpyF3I CTNAG 4 cut(s) 444, 452, 516, 567
Hsp92II CATG 3 cut(s) 73, 224, 343
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 2 cut(s) 394, 579
MaeI CTAG 1 cut(s) 643
MaeIII GTNAC 1 cut(s) 142
MboII GAAGA 7 cut(s) 22, 30, 116, 128, 163, 221, 338
MfeI CAATTG 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 2 cut(s) 107, 153
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 103
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 57
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 57
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 10, 79, 94
NcoI CCATGG 1 cut(s) 220
NlaIII CATG 3 cut(s) 73, 224, 343
NlaIV GGNNCC 1 cut(s) 66
NsiI ATGCAT 1 cut(s) 610
NspI RCATGY 1 cut(s) 343
OliI CACNNNNGTG 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 13, 86
PfeI GAWTC 2 cut(s) 149, 216
PkrI GCNGC 2 cut(s) 99, 238
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 64
RsaI GTAC 3 cut(s) 284, 413, 467
RsaNI GTAC 3 cut(s) 283, 412, 466
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 2 cut(s) 98, 237
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 1 cut(s) 187
SfaNI GCATC 2 cut(s) 394, 579
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 2 cut(s) 107, 153
SsiI CCGC 3 cut(s) 86, 95, 98
SspMI CTAG 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 2 cut(s) 177, 220
TaaI ACNGT 3 cut(s) 244, 599, 632
TaqI TCGA 2 cut(s) 7, 16
TasI AATT 2 cut(s) 107, 153
TatI WGTACW 2 cut(s) 282, 411
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 2 cut(s) 149, 216
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 3 cut(s) 192, 208, 247
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 3 cut(s) 180, 222, 518
TspRI CASTG 3 cut(s) 192, 208, 247
VpaK11BI GGWCC 1 cut(s) 64
XceI RCATGY 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 1 cut(s) 643
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.