MD04G1191600.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008214
Physical Location & Seq
Forward (+)
28098475 .. 28101255
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1191600.v1.1.491

Sequence Viewer

Length: 537 bp
ATGCCCCCGAAACGCAGAAACAAACCCGACCCGCATTTCGACACCCGATCCCCCGGCAAGAAACGGGCAGCTCCTCTGGAAGAAAATGGAGGAGAGAAAGTGCCGATTGTAACTCAAGAGAACTTTACCAAGGGAACACCGGTTGAGGTTTGCAGTGACGAAGATGGTTTCCACGGTGCTTGGTTTGCTGCAACCATAGTGGAAGCAGTGGGCAGTGACAAGTTCCTTATCGAGTACCAGAGCCTGAGGACAGAAGATGATTCGGCATTTCTGAGAGAAGAGATTGATACCCTGCACTTACGACCTAATCCGCCAGAAAATGCTGTTGAGCATTTCAGTTTGCTTCAAGAGGTTGATGCTTTGTACAATGATGGCTGGTGGGTGGGTATGATCTCAAAAGTTCTTAGCGGCTCAAGGTACATAGTCTACTTCAGGAGCACTTATGAAGAAATTGAATTTCAACAATCTGAGTTGAGGCTTCATCAGGACTGGATTGGTGGCAAATGGGTTATGCCTTCGCGGGCTTTAAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

179

Amino Acids

20.41

Weight (kDa)

5.17

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 45 - 105 2.9e-20 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 520
AciI CCGC 4 cut(s) 32, 311, 408, 520
AclWI GGATC 1 cut(s) 42
AcsI RAATTY 1 cut(s) 455
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 3 cut(s) 236, 365, 419
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 3 cut(s) 347, 455, 461
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw21I GWGCWC 1 cut(s) 440
AlwI GGATC 1 cut(s) 42
AlwNI CAGNNNCTG 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 68, 188
ApoI RAATTY 1 cut(s) 455
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AsiGI ACCGGT 1 cut(s) 139
AsuC2I CCSGG 1 cut(s) 54
AxyI CCTNAGG 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 80, 175
BccI CCATC 2 cut(s) 158, 365
BcnI CCSGG 1 cut(s) 54
BisI GCNGC 3 cut(s) 69, 189, 409
BlsI GCNGC 3 cut(s) 70, 190, 410
Bme1390I CCNGG 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 346
BpuEI CTTGAG 2 cut(s) 99, 397
BpuMI CCSGG 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 52, 129, 172
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 494
Bse21I CCTNAGG 1 cut(s) 245
BseDI CCNNGG 3 cut(s) 52, 129, 172
BseMII CTCAG 3 cut(s) 236, 263, 459
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 63, 105
BseXI GCAGC 2 cut(s) 80, 175
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 520
BshTI ACCGGT 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 2 cut(s) 54, 140
Bsp1286I GDGCHC 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 47, 390
BspACI CCGC 4 cut(s) 32, 311, 408, 520
BspCNI CTCAG 3 cut(s) 237, 264, 460
BspFNI CGCG 1 cut(s) 520
BspPI GGATC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 3 cut(s) 52, 129, 172
BssMI GATC 2 cut(s) 47, 390
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 4 cut(s) 245, 272, 404, 468
BstDSI CCRYGG 1 cut(s) 172
BstFNI CGCG 1 cut(s) 520
BstKTI GATC 2 cut(s) 50, 393
BstMBI GATC 2 cut(s) 47, 390
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 52
BstUI CGCG 1 cut(s) 520
BstV1I GCAGC 2 cut(s) 80, 175
Bsu36I CCTNAGG 1 cut(s) 245
BtgI CCRYGG 1 cut(s) 172
BtsI GCAGTG 3 cut(s) 160, 213, 220
BtsIMutI CAGTG 3 cut(s) 160, 213, 220
Cac8I GCNNGC 1 cut(s) 522
CaiI CAGNNNCTG 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 139
Csp6I GTAC 3 cut(s) 235, 364, 418
CspAI ACCGGT 1 cut(s) 139
CspCI CAANNNNNGTGG 4 cut(s) 161, 180, 196, 215
CviJI RGCY 6 cut(s) 71, 243, 375, 411, 478, 524
CviKI_1 RGCY 6 cut(s) 71, 243, 375, 411, 478, 524
CviQI GTAC 3 cut(s) 235, 364, 418
DdeI CTNAG 4 cut(s) 245, 272, 404, 468
DpnI GATC 2 cut(s) 49, 392
DpnII GATC 2 cut(s) 47, 390
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 415
Eco81I CCTNAGG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaiI YATR 5 cut(s) 197, 389, 422, 444, 512
FauI CCCGC 2 cut(s) 39, 513
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 69, 189, 409
Fsp4HI GCNGC 3 cut(s) 69, 189, 409
GluI GCNGC 3 cut(s) 69, 189, 409
HapII CCGG 2 cut(s) 54, 140
HinfI GANTC 1 cut(s) 260
HpaII CCGG 2 cut(s) 54, 140
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 2 cut(s) 273, 469
Hpy188III TCNNGA 5 cut(s) 77, 116, 347, 433, 485
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 153, 191, 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 4 cut(s) 245, 272, 404, 468
Kzo9I GATC 2 cut(s) 47, 390
LmnI GCTCC 2 cut(s) 76, 435
Lsp1109I GCAGC 2 cut(s) 80, 175
LweI GCATC 1 cut(s) 346
MaeIII GTNAC 3 cut(s) 109, 155, 215
MalI GATC 2 cut(s) 49, 392
MboI GATC 2 cut(s) 47, 390
MboII GAAGA 5 cut(s) 92, 173, 266, 290, 458
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 2 cut(s) 450, 455
MnlI CCTC 6 cut(s) 83, 84, 139, 240, 343, 468
MseI TTAA 1 cut(s) 527
MspI CCGG 2 cut(s) 54, 140
MspR9I CCNGG 1 cut(s) 54
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 1 cut(s) 185
NciI CCSGG 1 cut(s) 54
NdeII GATC 2 cut(s) 47, 390
NmuCI GTSAC 2 cut(s) 155, 215
PfeI GAWTC 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 3 cut(s) 70, 190, 410
PstNI CAGNNNCTG 1 cut(s) 244
RsaI GTAC 3 cut(s) 236, 365, 419
RsaNI GTAC 3 cut(s) 235, 364, 418
SaqAI TTAA 1 cut(s) 527
SatI GCNGC 3 cut(s) 69, 189, 409
Sau3AI GATC 2 cut(s) 47, 390
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 440
SetI ASST 5 cut(s) 73, 150, 307, 354, 419
SfaNI GCATC 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 114, 412
SmoI CTYRAG 2 cut(s) 114, 412
Sse9I AATT 2 cut(s) 450, 455
SsiI CCGC 4 cut(s) 32, 311, 408, 520
StyD4I CCNGG 1 cut(s) 52
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 2 cut(s) 39, 231
TasI AATT 2 cut(s) 450, 455
TatI WGTACW 1 cut(s) 363
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 527
Tru9I TTAA 1 cut(s) 527
TscAI CASTG 3 cut(s) 160, 213, 220
TseFI GTSAC 2 cut(s) 155, 215
TseI GCWGC 2 cut(s) 68, 188
Tsp45I GTSAC 2 cut(s) 155, 215
TspDTI ATGAA 2 cut(s) 459, 470
TspRI CASTG 3 cut(s) 160, 213, 220
XapI RAATTY 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.