pycom04g16920

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008214
Physical Location & Seq
Forward (+)
19470420 .. 19471595
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16920.2

Sequence Viewer

Length: 411 bp
ATGCCTCCGAAACGCAGAAACAAACCCGACCCGCATTTCGACACCCGATCCCCAGGCAAGAAACGGGCAGCTCCTCTGGAAGAAAATGGAGGAGAGAAAGTGCCGATTGTAACTCAAGAGAACTTTACCAATGGAACACCGGTTGAGGTTTGCAGTGATGAAGATGGTTTCCACGGTGCTTGGTTTGCTGCAACCATAGTGGAAGCAGTGGGCGGTGACAAGTTCCTTATCGAGTACCAGAGTCTGAGGACAGAAGATGATTCGGCATTTCTGAGAGAAGAGATTGATACCCTGCACATACGACCTAATCCACCAGAAAATGCTGTTGAGCATTTCAATTTGCTTCAAGAGGTTGATGCTTTGTACAATGATGGCTGGTGGGTGGGTGTGATCGATCTCAAAAGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

137

Amino Acids

15.27

Weight (kDa)

4.69

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 32, 213
AclWI GGATC 1 cut(s) 42
AfaI GTAC 2 cut(s) 236, 365
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 2 cut(s) 337, 347
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
AlwI GGATC 1 cut(s) 42
AlwNI CAGNNNCTG 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 68, 188
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AsiGI ACCGGT 1 cut(s) 139
AsuHPI GGTGA 1 cut(s) 227
BbvI GCAGC 2 cut(s) 80, 175
BccI CCATC 2 cut(s) 158, 365
BciT130I CCWGG 1 cut(s) 54
BisI GCNGC 2 cut(s) 69, 189
BlsI GCNGC 2 cut(s) 70, 190
Bme1390I CCNGG 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 52, 172
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 1 cut(s) 139
BseBI CCWGG 1 cut(s) 54
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 52, 172
BseMII CTCAG 2 cut(s) 236, 263
BseRI GAGGAG 2 cut(s) 63, 105
BseXI GCAGC 2 cut(s) 80, 175
BsgI GTGCAG 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 139
BshVI ATCGAT 1 cut(s) 393
BsiSI CCGG 1 cut(s) 140
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 3 cut(s) 47, 390, 394
BspACI CCGC 2 cut(s) 32, 213
BspCNI CTCAG 2 cut(s) 237, 264
BspDI ATCGAT 1 cut(s) 393
BspPI GGATC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 363
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 52, 172
BssMI GATC 3 cut(s) 47, 390, 394
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 363
BstDEI CTNAG 3 cut(s) 245, 272, 408
BstDSI CCRYGG 1 cut(s) 172
BstKTI GATC 3 cut(s) 50, 393, 397
BstMBI GATC 3 cut(s) 47, 390, 394
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 80, 175
Bsu15I ATCGAT 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 172
BtsI GCAGTG 2 cut(s) 160, 213
BtsIMutI CAGTG 2 cut(s) 160, 213
CaiI CAGNNNCTG 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 139
ClaI ATCGAT 1 cut(s) 393
Csp6I GTAC 2 cut(s) 235, 364
CspAI ACCGGT 1 cut(s) 139
CspCI CAANNNNNGTGG 4 cut(s) 161, 180, 196, 215
CviJI RGCY 2 cut(s) 71, 375
CviKI_1 RGCY 2 cut(s) 71, 375
CviQI GTAC 2 cut(s) 235, 364
DdeI CTNAG 3 cut(s) 245, 272, 408
DpnI GATC 3 cut(s) 49, 392, 396
DpnII GATC 3 cut(s) 47, 390, 394
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 52
FaiI YATR 2 cut(s) 197, 299
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 69, 189
Fsp4HI GCNGC 2 cut(s) 69, 189
GluI GCNGC 2 cut(s) 69, 189
HapII CCGG 1 cut(s) 140
HinfI GANTC 2 cut(s) 241, 260
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 227
Hpy188I TCNGA 3 cut(s) 9, 246, 273
Hpy188III TCNNGA 3 cut(s) 77, 116, 347
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 153, 191, 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 3 cut(s) 245, 272, 408
Kzo9I GATC 3 cut(s) 47, 390, 394
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 8 cut(s) 39, 62, 66, 153, 251, 305, 327, 361
Lsp1109I GCAGC 2 cut(s) 80, 175
LweI GCATC 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 109, 215
MalI GATC 3 cut(s) 49, 392, 396
MboI GATC 3 cut(s) 47, 390, 394
MboII GAAGA 4 cut(s) 92, 173, 266, 290
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 250
MnlI CCTC 6 cut(s) 15, 83, 84, 139, 240, 343
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeII GATC 3 cut(s) 47, 390, 394
NmuCI GTSAC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 2 cut(s) 70, 190
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 244
RsaI GTAC 2 cut(s) 236, 365
RsaNI GTAC 2 cut(s) 235, 364
SatI GCNGC 2 cut(s) 69, 189
Sau3AI GATC 3 cut(s) 47, 390, 394
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 54
SetI ASST 4 cut(s) 73, 150, 307, 354
SfaNI GCATC 1 cut(s) 346
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
Sse9I AATT 1 cut(s) 337
SsiI CCGC 2 cut(s) 32, 213
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 3 cut(s) 39, 231, 393
TasI AATT 1 cut(s) 337
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 260
TscAI CASTG 2 cut(s) 160, 213
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 2 cut(s) 68, 188
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 174
TspRI CASTG 2 cut(s) 160, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.