Rorug03G0021200

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008214
Physical Location & Seq
Forward (+)
1722325 .. 1724062
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0021200.1

Sequence Viewer

Length: 825 bp
ATGTACGACACGCTCAAAGACACCTACCAACTCTTCGAAGAAATCGGCCGCGGCCGATTCGGCACCATCCTCCGCGCCTTCTGCCCCGTCTCCGCCGAGTCTGTCGCCGTCAAGATCATCGATAAGTCCCTCCTCACCGACGAAACCGATCGTGCATGTCTTGAAAAAGAACCCAAGATCATGTCCCTCTTATCTCCCCACCCCAACATTCTCAAAATCCACAACGTTTTCGAAACAGAGGACTCCATGGCCATGGTGCTCGAGCTCTGCGAGCCCGAAACGCTCTACGACAAGATCGCTCACCGGCCGTTGCCGGAGCCGGAAGCCGCCGGTCTTATGAAACAGCTGTTGGGAGCTATAGCCCACTGCCACAGAATGGGTGTGGTGCACAGGGATCTGAAGCCGGAGAATGTTCTTTTTGATTCAAGAAACAATCTGAAGCTGGCGGATTTTGGGTCGGCGGAGTGGTCCGGTGAGGGAGGCTCCATGGAAGGTGTGGTGGGGACGCCGTACTACGTGGCGCCCGAGGTGTTGATGGGGAGGTTGTACAATGAGAAGGTGGATGTGTGGAGTGCGGGTGTGATGTTGTATATAATGTTGGGTGGGATTCCTCCATTTTACGGCGAAACGGCGACGGAGATCTTTGAGGCCGTTTTGAGAGGGAATCTAAGGTTTCCGCCGAAGGTTTTCCGGGGAGTTTCGCCGGCGGCGAAGGATCTGTTGAGGAAGATGATGTGTAGAGATGTTTCAAGAAGACTATCTGCAGAACAAGCACTTAGACACCCATGGATCTTGAGTGGTGGAGAAGCAAATCCAATGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

274

Amino Acids

30.61

Weight (kDa)

5.59

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 264 6.5e-71 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 11 - 257 5.1e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 520
AccB7I CCANNNNNTGG 1 cut(s) 376
AccII CGCG 2 cut(s) 51, 75
AclI AACGTT 1 cut(s) 225
AclWI GGATC 3 cut(s) 402, 723, 797
AcoI YGGCCR 4 cut(s) 46, 52, 249, 305
AcuI CTGAAG 2 cut(s) 419, 458
AcyI GRCGYC 2 cut(s) 506, 521
AfaI GTAC 3 cut(s) 5, 512, 548
AfiI CCNNNNNNNGG 2 cut(s) 376, 620
AgsI TTSAA 3 cut(s) 164, 426, 750
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 4 cut(s) 265, 346, 356, 442
AluI AGCT 4 cut(s) 265, 346, 356, 442
Alw21I GWGCWC 3 cut(s) 261, 267, 390
Alw26I GTCTC 1 cut(s) 94
Alw44I GTGCAC 1 cut(s) 386
AlwI GGATC 3 cut(s) 402, 723, 797
Ama87I CYCGRG 2 cut(s) 260, 524
AoxI GGCC 5 cut(s) 46, 52, 249, 305, 648
ApaLI GTGCAC 1 cut(s) 386
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 2 cut(s) 77, 523
AspS9I GGNCC 1 cut(s) 468
AsuC2I CCSGG 1 cut(s) 692
AsuHPI GGTGA 3 cut(s) 127, 293, 485
AsuII TTCGAA 2 cut(s) 36, 231
AvaI CYCGRG 2 cut(s) 260, 524
AvaII GGWCC 1 cut(s) 468
BaeGI GKGCMC 1 cut(s) 390
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 2 cut(s) 62, 520
BanII GRGCYC 2 cut(s) 267, 276
BbsI GAAGAC 1 cut(s) 760
Bbv12I GWGCWC 3 cut(s) 261, 267, 390
BccI CCATC 2 cut(s) 74, 529
BceAI ACGGC 6 cut(s) 92, 292, 493, 635, 637, 645
BcnI CCSGG 1 cut(s) 692
BcoDI GTCTC 1 cut(s) 94
BfmI CTRYAG 2 cut(s) 357, 762
BfoI RGCGCY 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 60
BglII AGATCT 1 cut(s) 639
BisI GCNGC 4 cut(s) 49, 52, 327, 708
BlsI GCNGC 4 cut(s) 50, 53, 328, 709
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 2 cut(s) 260, 524
BmgT120I GGNCC 1 cut(s) 468
BmiI GGNNCC 4 cut(s) 64, 318, 484, 522
BmrFI CCNGG 1 cut(s) 692
BpiI GAAGAC 1 cut(s) 760
BplI GAGNNNNNCTC 2 cut(s) 467, 499
Bpu14I TTCGAA 2 cut(s) 36, 231
BpuEI CTTGAG 1 cut(s) 814
BpuMI CCSGG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 517
BsaHI GRCGYC 2 cut(s) 506, 521
BsaJI CCNNGG 7 cut(s) 49, 246, 252, 486, 525, 691, 785
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 620
Bse118I RCCGGY 3 cut(s) 303, 329, 703
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 7 cut(s) 49, 246, 252, 486, 525, 691, 785
BseGI GGATG 2 cut(s) 66, 568
BseLI CCNNNNNNNGG 2 cut(s) 376, 620
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 390
BseX3I CGGCCG 3 cut(s) 46, 52, 305
Bsh1236I CGCG 2 cut(s) 51, 75
Bsh1285I CGRYCG 4 cut(s) 49, 55, 151, 308
BshFI GGCC 5 cut(s) 48, 54, 251, 307, 650
BshNI GGYRCC 2 cut(s) 62, 520
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 4 cut(s) 49, 55, 151, 308
BsiHKAI GWGCWC 3 cut(s) 261, 267, 390
BsiHKCI CYCGRG 2 cut(s) 260, 524
BsiSI CCGG 8 cut(s) 304, 314, 320, 330, 404, 471, 691, 704
BslFI GGGAC 3 cut(s) 112, 169, 517
BslI CCNNNNNNNGG 2 cut(s) 376, 620
BsmAI GTCTC 1 cut(s) 94
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 3 cut(s) 112, 169, 517
BsnI GGCC 5 cut(s) 48, 54, 251, 307, 650
BsoBI CYCGRG 2 cut(s) 260, 524
Bsp119I TTCGAA 2 cut(s) 36, 231
Bsp1286I GDGCHC 4 cut(s) 261, 267, 276, 390
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
Bsp19I CCATGG 4 cut(s) 246, 252, 486, 785
BspANI GGCC 5 cut(s) 48, 54, 251, 307, 650
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 2 cut(s) 51, 75
BspLI GGNNCC 4 cut(s) 64, 318, 484, 522
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 3 cut(s) 402, 723, 797
BspT104I TTCGAA 2 cut(s) 36, 231
BspT107I GGYRCC 2 cut(s) 62, 520
BsrFI RCCGGY 3 cut(s) 303, 329, 703
BsrGI TGTACA 1 cut(s) 546
BssAI RCCGGY 3 cut(s) 303, 329, 703
BssECI CCNNGG 7 cut(s) 49, 246, 252, 486, 525, 691, 785
BssMI GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
BssNI GRCGYC 2 cut(s) 506, 521
BssT1I CCWWGG 4 cut(s) 246, 252, 486, 785
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 2 cut(s) 506, 521
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 517
BstBI TTCGAA 2 cut(s) 36, 231
BstC8I GCNNGC 3 cut(s) 272, 444, 705
BstDEI CTNAG 2 cut(s) 668, 776
BstDSI CCRYGG 5 cut(s) 49, 246, 252, 486, 785
BstF5I GGATG 2 cut(s) 66, 568
BstFNI CGCG 2 cut(s) 51, 75
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 2 cut(s) 77, 523
BstKTI GATC 8 cut(s) 117, 151, 180, 297, 397, 642, 718, 792
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
BstMCI CGRYCG 4 cut(s) 49, 55, 151, 308
BstMWI GCNNNNNNNGC 5 cut(s) 60, 81, 271, 280, 770
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 690
BstSFI CTRYAG 2 cut(s) 357, 762
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 2 cut(s) 51, 75
BstV2I GAAGAC 1 cut(s) 760
BstX2I RGATCY 4 cut(s) 394, 639, 715, 789
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 4 cut(s) 394, 639, 715, 789
BstZI CGGCCG 3 cut(s) 46, 52, 305
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 5 cut(s) 48, 54, 251, 307, 650
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 5 cut(s) 49, 246, 252, 486, 785
BtsCI GGATG 2 cut(s) 66, 568
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 3 cut(s) 272, 444, 705
CfoI GCGC 2 cut(s) 77, 523
Cfr10I RCCGGY 3 cut(s) 303, 329, 703
Cfr13I GGNCC 1 cut(s) 468
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 1 cut(s) 514
Csp6I GTAC 3 cut(s) 4, 511, 547
CviAII CATG 6 cut(s) 156, 181, 247, 253, 487, 786
CviQI GTAC 3 cut(s) 4, 511, 547
DdeI CTNAG 2 cut(s) 668, 776
DinI GGCGCC 1 cut(s) 522
DpnI GATC 8 cut(s) 116, 150, 179, 296, 396, 641, 717, 791
DpnII GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
EaeI YGGCCR 4 cut(s) 46, 52, 249, 305
EagI CGGCCG 3 cut(s) 46, 52, 305
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EciI GGCGGA 4 cut(s) 82, 461, 476, 666
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 3 cut(s) 46, 52, 305
Eco130I CCWWGG 4 cut(s) 246, 252, 486, 785
Eco24I GRGCYC 2 cut(s) 267, 276
Eco47I GGWCC 1 cut(s) 468
Eco52I CGGCCG 3 cut(s) 46, 52, 305
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 419, 458
Eco88I CYCGRG 2 cut(s) 260, 524
EcoICRI GAGCTC 1 cut(s) 265
EcoT14I CCWWGG 4 cut(s) 246, 252, 486, 785
EcoT38I GRGCYC 2 cut(s) 267, 276
EgeI GGCGCC 1 cut(s) 522
EheI GGCGCC 1 cut(s) 522
ErhI CCWWGG 4 cut(s) 246, 252, 486, 785
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 6 cut(s) 159, 184, 250, 256, 490, 789
FaqI GGGAC 3 cut(s) 112, 169, 517
FatI CATG 6 cut(s) 155, 180, 246, 252, 486, 785
FauI CCCGC 1 cut(s) 568
Fnu4HI GCNGC 4 cut(s) 49, 52, 327, 708
FokI GGATG 2 cut(s) 53, 575
FriOI GRGCYC 2 cut(s) 267, 276
Fsp4HI GCNGC 4 cut(s) 49, 52, 327, 708
GlaI GCGC 2 cut(s) 76, 522
GluI GCNGC 4 cut(s) 49, 52, 327, 708
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 5 cut(s) 48, 54, 251, 307, 650
HapII CCGG 8 cut(s) 304, 314, 320, 330, 404, 471, 691, 704
HgaI GACGC 1 cut(s) 514
HhaI GCGC 2 cut(s) 77, 523
Hin1I GRCGYC 2 cut(s) 506, 521
Hin1II CATG 6 cut(s) 159, 184, 250, 256, 490, 789
Hin6I GCGC 2 cut(s) 75, 521
HinP1I GCGC 2 cut(s) 75, 521
HinfI GANTC 6 cut(s) 57, 98, 242, 422, 607, 664
HpaII CCGG 8 cut(s) 304, 314, 320, 330, 404, 471, 691, 704
HphI GGTGA 3 cut(s) 127, 293, 485
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 399, 438
Hpy188III TCNNGA 5 cut(s) 112, 161, 426, 750, 793
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 2 cut(s) 143, 637
HpyAV CCTTC 5 cut(s) 88, 485, 550, 676, 706
HpyCH4IV ACGT 2 cut(s) 225, 516
HpyCH4V TGCA 3 cut(s) 155, 388, 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 81, 271, 280, 770
HpyF3I CTNAG 2 cut(s) 668, 776
HpySE526I ACGT 2 cut(s) 225, 516
Hsp92I GRCGYC 2 cut(s) 506, 521
Hsp92II CATG 6 cut(s) 159, 184, 250, 256, 490, 789
HspAI GCGC 2 cut(s) 75, 521
KasI GGCGCC 1 cut(s) 520
KroI GCCGGC 1 cut(s) 703
KroNI GCCGGC 1 cut(s) 705
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
LmnI GCTCC 3 cut(s) 316, 353, 488
MaeII ACGT 2 cut(s) 225, 516
MalI GATC 8 cut(s) 116, 150, 179, 296, 396, 641, 717, 791
MboI GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
MboII GAAGA 4 cut(s) 25, 50, 739, 765
MflI RGATCY 4 cut(s) 394, 639, 715, 789
MhlI GDGCHC 4 cut(s) 261, 267, 276, 390
MlsI TGGCCA 1 cut(s) 251
MluNI TGGCCA 1 cut(s) 251
Mly113I GGCGCC 1 cut(s) 521
MlyI GAGTC 2 cut(s) 107, 236
Mox20I TGGCCA 1 cut(s) 251
MreI CGCCGGCG 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 51, 346
MspI CCGG 8 cut(s) 304, 314, 320, 330, 404, 471, 691, 704
MspR9I CCNGG 1 cut(s) 692
MvnI CGCG 2 cut(s) 51, 75
MwoI GCNNNNNNNGC 5 cut(s) 60, 81, 271, 280, 770
NaeI GCCGGC 1 cut(s) 705
NarI GGCGCC 1 cut(s) 521
NciI CCSGG 1 cut(s) 692
NcoI CCATGG 4 cut(s) 246, 252, 486, 785
NdeII GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
NgoMIV GCCGGC 1 cut(s) 703
NlaIII CATG 6 cut(s) 159, 184, 250, 256, 490, 789
NlaIV GGNNCC 4 cut(s) 64, 318, 484, 522
NmeAIII GCCGAG 1 cut(s) 121
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 2 cut(s) 36, 231
PaeR7I CTCGAG 1 cut(s) 260
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 522, 707
PdiI GCCGGC 1 cut(s) 705
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 4 cut(s) 57, 422, 607, 664
PflMI CCANNNNNTGG 1 cut(s) 376
PkrI GCNGC 4 cut(s) 50, 53, 328, 709
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 2 cut(s) 106, 236
PluTI GGCGCC 1 cut(s) 524
PpsI GAGTC 2 cut(s) 106, 236
Ppu21I YACGTR 1 cut(s) 517
Psp124BI GAGCTC 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 225
PspN4I GGNNCC 4 cut(s) 64, 318, 484, 522
PspPI GGNCC 1 cut(s) 468
PspXI VCTCGAGB 1 cut(s) 260
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 4 cut(s) 394, 639, 715, 789
PvuI CGATCG 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 3 cut(s) 5, 512, 548
RsaNI GTAC 3 cut(s) 4, 511, 547
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 52
SatI GCNGC 4 cut(s) 49, 52, 327, 708
Sau3AI GATC 8 cut(s) 114, 148, 177, 294, 394, 639, 715, 789
Sau96I GGNCC 1 cut(s) 468
SchI GAGTC 2 cut(s) 107, 236
ScrFI CCNGG 1 cut(s) 692
SduI GDGCHC 4 cut(s) 261, 267, 276, 390
SfcI CTRYAG 2 cut(s) 357, 762
SfoI GGCGCC 1 cut(s) 522
Sfr274I CTCGAG 1 cut(s) 260
Sfr303I CCGCGG 1 cut(s) 52
SfuI TTCGAA 2 cut(s) 36, 231
SgrAI CRCCGGYG 1 cut(s) 703
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 468
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 260, 793
SmoI CTYRAG 2 cut(s) 260, 793
SspDI GGCGCC 1 cut(s) 520
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 690
StyI CCWWGG 4 cut(s) 246, 252, 486, 785
TaiI ACGT 2 cut(s) 228, 519
TaqI TCGA 4 cut(s) 36, 120, 231, 261
TatI WGTACW 1 cut(s) 546
TauI GCSGC 4 cut(s) 51, 54, 329, 710
TfiI GAWTC 4 cut(s) 57, 422, 607, 664
TscAI CASTG 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 376
VneI GTGCAC 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 468
XceI RCATGY 1 cut(s) 159
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.