Rroxscaffold_6G00421750

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008214
Physical Location & Seq
Forward (+)
42832897 .. 42835369
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421750.1

Sequence Viewer

Length: 690 bp
ATGCCTCGAAAGCCTCGAAGAACCAAAGTCTTCAACTTCAGTAAATGCCTGACTCCTGGTTTGGTCCCCATGCCGGAAACGCCTCCGCCGTCTCCGCCGCGTGAAGAATTGATGGAAGAAGTTGGAGAGATCCTGACCAGGCTGGCAAGTACTCAAAGACAGAAACTTTATAGGGTTTGCATTATCATAAATGACCCTAATGTAACTGATTCAATTGTAAGCAATGAAATCTTCACCAAGGGAGCACTGGTTGAGGTTAGCAGTGATGAAGAAGGATTCCATGGTTCTTGGTTTGCTGCCACTGTAGTGAAATCTGTGGGGAAAGACAAGTTCCTTATTGAGTACAAGTGCTTGAGGACAGAGGATGATTTGGCATTTTTGAGAGAAGAGGTTGATAACATGCACATACGACCTCATCCGCCAGAAAATGTTGTTGATCGTTTCAAGCAACTTGACGAGGTTGATGCTTTGTACAATGATGGGTGGTGGGTTGGTGTTATTTCTAAGGTTCTAAGAGGTTCAAGGTACATAGTCTACTTCAAGGAAACCTGTGAGGAACTTGAATATCAGCACTCTGAGTTAAGGCTTCATCAGGACTGGATTGATGGCAAATGGGTTATCCCATCTCGGGCGTCAATGTCTAATTCTGATGATCGAGTTAAAGTTATAGTTCTGTGGAAAATGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

229

Amino Acids

26.58

Weight (kDa)

5.53

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 81 - 141 2.9e-20 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 534
AccII CGCG 1 cut(s) 100
AciI CCGC 4 cut(s) 86, 95, 98, 419
AclWI GGATC 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 4 cut(s) 151, 344, 473, 527
AfiI CCNNNNNNNGG 2 cut(s) 73, 628
AgsI TTSAA 6 cut(s) 34, 213, 445, 522, 541, 563
AjnI CCWGG 2 cut(s) 55, 137
AleI CACNNNNGTG 1 cut(s) 305
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 124
Ama87I CYCGRG 1 cut(s) 627
ApeKI GCWGC 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 627
AvaII GGWCC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 283
BccI CCATC 4 cut(s) 106, 473, 599, 631
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 2 cut(s) 57, 139
BcoDI GTCTC 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 2 cut(s) 98, 297
BlsI GCNGC 2 cut(s) 99, 298
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 57, 139
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 627
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 2 cut(s) 57, 139
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 237, 280
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 628
Bse1I ACTGG 2 cut(s) 252, 602
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 57, 139
BseDI CCNNGG 2 cut(s) 237, 280
BseGI GGATG 2 cut(s) 370, 415
BseLI CCNNNNNNNGG 2 cut(s) 73, 628
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 2 cut(s) 252, 602
BseXI GCAGC 1 cut(s) 283
Bsh1236I CGCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 73, 628
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 627
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 3 cut(s) 129, 436, 652
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 4 cut(s) 86, 95, 98, 419
BspCNI CTCAG 1 cut(s) 568
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 229
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 2 cut(s) 252, 602
BssECI CCNNGG 2 cut(s) 237, 280
BssMI GATC 3 cut(s) 129, 436, 652
BssNI GRCGYC 1 cut(s) 632
BssT1I CCWWGG 2 cut(s) 237, 280
Bst2UI CCWGG 2 cut(s) 57, 139
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 632
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 3 cut(s) 504, 512, 576
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 370, 415
BstFNI CGCG 1 cut(s) 100
BstKTI GATC 3 cut(s) 132, 439, 655
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 3 cut(s) 129, 436, 652
BstMWI GCNNNNNNNGC 3 cut(s) 10, 79, 94
BstNI CCWGG 2 cut(s) 57, 139
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 55, 137
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 370, 415
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 3 cut(s) 245, 268, 300
Cac8I GCNNGC 1 cut(s) 144
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 621
Csp6I GTAC 4 cut(s) 150, 343, 472, 526
CviAII CATG 3 cut(s) 70, 281, 400
CviJI RGCY 3 cut(s) 13, 142, 586
CviKI_1 RGCY 3 cut(s) 13, 142, 586
CviQI GTAC 4 cut(s) 150, 343, 472, 526
DdeI CTNAG 3 cut(s) 504, 512, 576
DpnI GATC 3 cut(s) 131, 438, 654
DpnII GATC 3 cut(s) 129, 436, 652
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 3 cut(s) 75, 84, 408
Eco130I CCWWGG 2 cut(s) 237, 280
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 55, 137
EcoT14I CCWWGG 2 cut(s) 237, 280
ErhI CCWWGG 2 cut(s) 237, 280
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 3 cut(s) 73, 284, 403
FaiI YATR 8 cut(s) 71, 171, 188, 282, 401, 407, 530, 668
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 69, 280, 399
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 2 cut(s) 98, 297
FokI GGATG 2 cut(s) 377, 402
Fsp4HI GCNGC 2 cut(s) 98, 297
GluI GCNGC 2 cut(s) 98, 297
HapII CCGG 1 cut(s) 74
HgaI GACGC 1 cut(s) 621
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 3 cut(s) 73, 284, 403
HinfI GANTC 3 cut(s) 52, 209, 276
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 2 cut(s) 577, 649
Hpy188III TCNNGA 2 cut(s) 133, 593
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 180, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 79, 94
HpyF3I CTNAG 3 cut(s) 504, 512, 576
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 3 cut(s) 73, 284, 403
Kzo9I GATC 3 cut(s) 129, 436, 652
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 1 cut(s) 454
MaeIII GTNAC 1 cut(s) 202
MalI GATC 3 cut(s) 131, 438, 654
MboI GATC 3 cut(s) 129, 436, 652
MboII GAAGA 7 cut(s) 22, 30, 116, 128, 223, 281, 398
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 3 cut(s) 107, 213, 643
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 103
MseI TTAA 2 cut(s) 581, 660
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 57, 139
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 2 cut(s) 57, 139
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 10, 79, 94
NcoI CCATGG 1 cut(s) 280
NdeII GATC 3 cut(s) 129, 436, 652
NlaIII CATG 3 cut(s) 73, 284, 403
NlaIV GGNNCC 1 cut(s) 66
NspI RCATGY 1 cut(s) 403
OliI CACNNNNGTG 1 cut(s) 305
PcsI WCGNNNNNNNCGW 2 cut(s) 13, 86
PfeI GAWTC 2 cut(s) 209, 276
PkrI GCNGC 2 cut(s) 99, 298
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 55, 137
PspGI CCWGG 2 cut(s) 55, 137
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 64
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 4 cut(s) 151, 344, 473, 527
RsaNI GTAC 4 cut(s) 150, 343, 472, 526
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 581, 660
SatI GCNGC 2 cut(s) 98, 297
Sau3AI GATC 3 cut(s) 129, 436, 652
Sau96I GGNCC 1 cut(s) 64
ScaI AGTACT 1 cut(s) 151
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 57, 139
SduI GDGCHC 1 cut(s) 247
SetI ASST 8 cut(s) 258, 393, 415, 462, 510, 520, 527, 551
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
Sse9I AATT 3 cut(s) 107, 213, 643
SsiI CCGC 4 cut(s) 86, 95, 98, 419
StyD4I CCNGG 2 cut(s) 55, 137
StyI CCWWGG 2 cut(s) 237, 280
TaaI ACNGT 1 cut(s) 304
TaqI TCGA 3 cut(s) 7, 16, 655
TasI AATT 3 cut(s) 107, 213, 643
TatI WGTACW 3 cut(s) 149, 342, 471
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 2 cut(s) 209, 276
Tru1I TTAA 2 cut(s) 581, 660
Tru9I TTAA 2 cut(s) 581, 660
TscAI CASTG 3 cut(s) 252, 268, 307
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 240, 282, 578
TspRI CASTG 3 cut(s) 252, 268, 307
VpaK11BI GGWCC 1 cut(s) 64
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 534
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.