RLG00000025214

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008214
Physical Location & Seq
Forward (+)
43028672 .. 43031204
2533 bp
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UTR
Exon/CDS
Intron
RLM00000025214

Sequence Viewer

Length: 375 bp
ATGCCGGAAACGCCTCCGCCGTCTCCGCCGCGTGAAGAATTGATAGAAGAAGTTGGAGACGTCCGAACAAATGAACCTAATGTAACTGATTCAATTGTGAGCAATGAAATCTTCACCAAGGGAGCACTGGTTGAGGTTAGCAGTGATGAAGAAGGATTCCATGGTTCTTGGTTTGCTGCCACTGTAGTGAAATCTGTGGGGAAAGACAAGTTCCTTATTGAGTACAAGTGCTTGAGGACAGAGGATGATTTGGCATTTTTGAGAGAAGAGGTTGATAACATGCACATACGACCTCATCCACCAGAGAAGAGGTTGATAAGATGCGCATACTACATTGTGTGGCTTATTCTGATGGCGACATTTATTCTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

125

Amino Acids

14.22

Weight (kDa)

4.76

Isoelectric Point (pI)

68.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 41 - 101 7.6e-21 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc16I TGCGCA 1 cut(s) 325
AccII CGCG 1 cut(s) 31
AciI CCGC 3 cut(s) 17, 26, 29
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 1 cut(s) 93
AleI CACNNNNGTG 1 cut(s) 185
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 2 cut(s) 27, 51
ApeKI GCWGC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 163
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 4
BcoDI GTCTC 2 cut(s) 27, 51
BfaI CTAG 1 cut(s) 373
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 2 cut(s) 29, 177
BlsI GCNGC 2 cut(s) 30, 178
BmsI GCATC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 117, 160
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 117, 160
BseGI GGATG 2 cut(s) 250, 295
BseMI GCAATG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 5
BsmAI GTCTC 2 cut(s) 27, 51
BsmBI CGTCTC 2 cut(s) 27, 51
Bsp1286I GDGCHC 1 cut(s) 127
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 3 cut(s) 17, 26, 29
BspFNI CGCG 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 2 cut(s) 117, 160
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 117, 160
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 261, 302
BstACI GRCGYC 1 cut(s) 60
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 250, 295
BstFNI CGCG 1 cut(s) 31
BstHHI GCGC 1 cut(s) 326
BstMAI GTCTC 2 cut(s) 27, 51
BstMWI GCNNNNNNNGC 2 cut(s) 10, 25
BstNSI RCATGY 1 cut(s) 283
BstSFI CTRYAG 1 cut(s) 183
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 2 cut(s) 250, 295
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 3 cut(s) 125, 148, 180
CfoI GCGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 161, 280
CviJI RGCY 1 cut(s) 343
CviKI_1 RGCY 1 cut(s) 343
CviQI GTAC 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 261, 302
EarI CTCTTC 2 cut(s) 261, 302
EciI GGCGGA 2 cut(s) 6, 15
Eco130I CCWWGG 2 cut(s) 117, 160
EcoT14I CCWWGG 2 cut(s) 117, 160
ErhI CCWWGG 2 cut(s) 117, 160
Esp3I CGTCTC 2 cut(s) 27, 51
FaeI CATG 2 cut(s) 164, 283
FaiI YATR 4 cut(s) 162, 281, 287, 328
FatI CATG 2 cut(s) 160, 279
Fnu4HI GCNGC 2 cut(s) 29, 177
FokI GGATG 2 cut(s) 257, 282
Fsp4HI GCNGC 2 cut(s) 29, 177
FspAI RTGCGCAY 1 cut(s) 325
FspBI CTAG 1 cut(s) 373
FspI TGCGCA 1 cut(s) 325
GlaI GCGC 1 cut(s) 325
GluI GCNGC 2 cut(s) 29, 177
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 164, 283
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 2 cut(s) 89, 156
HpaII CCGG 1 cut(s) 5
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 65, 351
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 25
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 164, 283
HspAI GCGC 1 cut(s) 324
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 3 cut(s) 18, 113, 315
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 311
MaeI CTAG 1 cut(s) 373
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 82
MboII GAAGA 7 cut(s) 47, 59, 103, 161, 278, 319, 361
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 2 cut(s) 38, 93
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 7 cut(s) 24, 127, 228, 235, 262, 303, 303
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 1 cut(s) 5
MunI CAATTG 1 cut(s) 93
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 2 cut(s) 10, 25
NcoI CCATGG 1 cut(s) 160
NlaIII CATG 2 cut(s) 164, 283
NsbI TGCGCA 1 cut(s) 325
NspI RCATGY 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PfeI GAWTC 2 cut(s) 89, 156
PkrI GCNGC 2 cut(s) 30, 178
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 185
SatI GCNGC 2 cut(s) 29, 177
SduI GDGCHC 1 cut(s) 127
SetI ASST 6 cut(s) 63, 79, 138, 273, 295, 314
SfaNI GCATC 1 cut(s) 311
SfcI CTRYAG 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 2 cut(s) 38, 93
SsiI CCGC 3 cut(s) 17, 26, 29
SspMI CTAG 1 cut(s) 373
StyI CCWWGG 2 cut(s) 117, 160
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 63
TasI AATT 2 cut(s) 38, 93
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 2 cut(s) 89, 156
TscAI CASTG 3 cut(s) 132, 148, 187
TseI GCWGC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 87, 120, 162
TspRI CASTG 3 cut(s) 132, 148, 187
XceI RCATGY 1 cut(s) 283
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XspI CTAG 1 cut(s) 373
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.