AT3G06880

E3 ubiquitin-protein ligase LIN-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010154
Physical Location & Seq
Reverse (-)
2169768 .. 2175771
6004 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06880.1

Sequence Viewer

Length: 3786 bp
ATGGCTTACCTTTCATCTTCTCAATCGACTTTTATCTCATGTAGTGAGAATTCATCTCTTGATTCGATTCTAGTTTCAATCAACGTCTACATCCTCGGCGTCATATCAGACCCTGAAGCATGGATTTCGCTTAAGCAGAAATGCATCACATTGCTCAGCATTGAAGAAGATAATACTCTGTTTGAATTCTCCTCTGAACACTCTGCTCTCTCCAATCTCTATTGGGGAATCGATAGCATCGAAGCTTCTATACATCCCGAATGCTCAGAAGAAAAGACGTCTCGTCTGAGAAACTCAGAGAGAATGCTTCAAATGCCTGCGTTGCTTGACGAACAAGGGACCACCACTTCTGGTGTTCCAAACACAGTCTTGATTTCTTTCTCTTACTTTTACCTCTCCATTGTAAGCTATCTTCAAGGTGATTCCTTGCAGTCCACATTGCATTTTCTGCAGTCGGTTTTGGTCTCGCCGGAGATTGTAAGAACCGACATTGCCCCTGAACTCTGCGAGAGCATTTTCTTCACTCCTGGTGTGTACAAGTCAGATGAAGAGATCAGAGAGATTGCAAGAAAGTATAAATACAGAGCAACTTATTACCAAGTTATGTCCTATGGAGAGACTCATCAGCCGCCACGTGAATGTATAGAGACACCGGTTAGAAGGCAGAAGGAATATGGGCAAGAAATATTTGCGGCTAATGCACATTCAGTTGCAGAGAAGCTAGAGTTGTCAGAAACTTGTGAGAAGTTATTGCAGTACCAGAACCTCCACAGTGTTGATTTGCAAGAGGAGGAACTTAATGACATTTTCAACAAAATCAAAGCATCAAGGAAGATTGAGAAATCTGAAAATAACTTCGAAGGTTCTCAATGTTTGGATTGTAACCTGCAGGAAGATTACAATGCAGAGCCTGGAAAAAGTACAAGAGTCAGATGCCTTAATGAATTCTTGAACGAGTCTCAGCCAGATACTAGAGAAGACATTGGCACGGACACATTAGCAAACATCTTCTGTGTTTCCCAACAACAAGCTCACAAGGAAGCAAACAAAGCTTATAATGAGGACACATTAGCCAACAGATCCAGCAGTTTTATCGGTAATTTTAACCGTTCTATCTTTGAAATTCAGGCTCAACAATCAAAGACAACTTGGAATACCCACTTAGAAGATGCATCTTCTTTGAGGCAACTAGATTTGGAAGAAATATCAGTATTTGGACAAAAAGGGTCCATAAGTTTTGAAGGAATGAGGAGAAATTTGCAGACCAAAAAACGAGGGAATGGACTTGAGACGCTTTCTAGAAGAGCTCCAACAATGGATTTATGGATGAACCTTCAGAGCTTGATAAAGGAAGTGTTAGGGAATGCAGATGAGAAATATGTCTCAGAGGTTACAATGATATATCAAATGCTGAACAGGAAAGAAGGATTCAAGTATAGCATGCTAAAAGATGTCATTCTGGATCAGCTGTTTACGGCTATTTCTAGTTCCGAGGAGAAGACCGTGATTAAGGCGTCGATGACGGCACTTACCAAGATCATATCAGTTAATAGAACAGCTCTTGAGGAGGTCAAAAGGAAGGGTTTAAATTTAAGTCATTTAGCAAATGCTCTCAAACAAAATGTGCAAGAAGCAGCTATTCTCATCTACCTGATAAAGCCATCTCCAACAGAAATAAAGAGTCTTGAGCTTTTACCAGCTCTTGTTGATGTTGTTGCATCCACTTCTTCTTCATCTTCTTGTTACACATTTATACCTTCACCTTCTTATTTGACGCCGCCTGCAGCATCATTGATGATAATTGAAGTGCTTATCACTGCTTTTGATCATGCTACAAACACAATGCACTTGGCTGCAATCAGCTCTCCTTCTGTCCTTTGTGGACTTCTTGATGTTGCAAAGAGTGGAAACTCAGGGGAATTCATCTCCTTGACAAGTATTTTAGTAAAATGCATGCAATTTGATGGACTTCACAGGAAGTATATTTATCAGCATACTCGAGTGGCTCCTTTTGCGCATCTCCTGCAAAGCAAAGACCAAGAAGAAATTTGCATTGCTCTTCAATTTCTTCATGAGGTCCTGAAGATACCAAGGTCATCAGCAATCAAGATTCTGCAACAGATAAAGAAAGAAGGAAGTTTTGACATTAAGGTCACATTATTGCACTGTATTAAACACTTGCAGGGTGATCACAAACTCTTTGCAGCAGATATATTGCTTCAGTTAAATGCACTGGATTCTCCACCTGAGAACAAAAAGTACAGAAATGAAGCTACCAGAGCTCTACTTGATGCAGTTACATATAGTGAAGGTTCAAATATGCAGCTTTTATCAACGTTCATTCTTGCGAATATCGGTGGAACTTATTCATGGACAGGAGAACCTTACACTGCTGCTTGGCTAATGAAAAGAGGAGGTCTAACTTCTATGTCACATATGAATATGATAAGAAACATCAACTGGTCAGATGAATGCTTGCAGGATACAGGAATAGATGGATGGTGTTGTAAGATAGCTAGAAGAATTATTGATACAGGCAAAGCCACGTTTTGTGGTTTACAAGAAGGGCTAAAGAGTAAAAACAAGAGTGTTTCAAAAGCATGTCTTATAGCCATTGCATGGCTCAGTATAGAGATTTCAAAAGGTCCAAATAGTTTAAAATACTCAGCATGTGAAGTTTTACTTGATGAGGTAGCACAATTTCTCCACCCTGGTTTGGAGCTTGAAGAAAGACTTCTTGCTTGTATATGCATCTATAATTTTAGCTCTGGCAAAGGAATCCACAAACTAGTCAATTTCTCGGAAGGAGTTAGAGAGTCTTTGAGACGTCTTTCACATGTGACTTGGATGGCAGACGAACTGCATAAAGCAACATATTATCTATTCAGCAAATCTGACCAACGCATATCTTGTGTTCATACACAAACCGTAGAGATGCATCAATCTGGCAGTGGAGCTGTAACAGCACTCATCTACCATAAAGGCTTGCTCTTTAGTGGATTCTCAGATGGTTCAATCAGGGTGTGGAATGTGAATAAGAAAATAGCTACGCTTTTATGGGACATCAAGGAGCACAAAAGCACAGTAACATGCTTTTCACTCTCTGAAACAGGAGAGTGTGTCTTAAGTGGATCAGCAGATAAAACTATCAGGGTATGGCAGATAGTTAAAGGAAAACTGGAATGTGCTGAAGTCATAAAAACAAAAGATTCAATAAGGAAACTGGAAGCATTCGGAAATATGATCTTTGTTATAACAAAAGGGCACAAGATGAAGCTGCTTGATTCATCAAGAATATCTCAAAGTATCTTCAAAGGTAAAGGTGTGAAGAGTATGGTATCAGCTCAAGGAAAGATTTATATAGGATGCATAGATACAAGTATACAGGAATTAATTGTTGCAAATAAACGGGAGAAAGAGATTAAAGCACCGACGAGGAGCTGGAGACTTCAAAACAAGCCTATCAATTCAGTGGTTGTGTACAAAGATATGTTATATAGCTCAAGCACATATGTTGAGATGTCTAATATAAAGGATCTGAGAAGGAACTATGAGCCCCAAATGTCAATAACAGCAGAAAAGGGGTCTAATATAGTAGCAATGGGTGTTGTTGAAGACTTCATCTACCTGAATCGAAGTTCATCCGCAAACACTCTTCAGATTTGGTTGAGAAGGACACAACAGAAAGTGGGAAGACTATCAGCAGGAAGCAAGATAACAAGCCTCCTTACTGCTAATGACATTGTTTTTTGCGGTACAGAAGCTGGGCTCATGGACCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1261

Amino Acids

141.41

Weight (kDa)

6.66

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 24 - 174 3.3e-22 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 451 - 602 2.4e-37 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 604 - 956 9.3e-50 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR5 PF25175 916 - 1141 1e-11 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 927 - 1057 7.4e-08 CDC20/Fizzy WD40 domain
NBCH_WD40 PF20426 971 - 1069 9.7e-06 Neurobeachin beta propeller domain
Beta-prop_WDR3_1st PF25173 977 - 1075 4.5e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 979 - 1075 1.4e-09 Prp19 WD40 domain
Beta-prop_EML PF23409 980 - 1088 8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd PF25047 990 - 1056 2.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 991 - 1055 2.8e-06 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 992 - 1089 5.5e-06 WDHD1 first WD40 domain
EIF3I PF24805 995 - 1195 3.3e-06 EIF3I
Beta-prop_THOC3 PF25174 997 - 1099 7.8e-10 THOC3 beta-propeller domain
WD40 PF00400 1019 - 1053 7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1056, 3254
AasI GACNNNNNNGTC 1 cut(s) 2150
AatII GACGTC 2 cut(s) 281, 2830
Acc16I TGCGCA 1 cut(s) 2016
Acc36I ACCTGC 1 cut(s) 894
AccB7I CCANNNNNTGG 1 cut(s) 2619
AccI GTMKAC 2 cut(s) 87, 3382
AciI CCGC 5 cut(s) 629, 692, 1778, 3645, 3753
AclI AACGTT 1 cut(s) 2336
AclWI GGATC 4 cut(s) 1074, 1470, 3139, 3543
AcsI RAATTY 8 cut(s) 49, 185, 944, 1122, 1255, 1588, 1919, 2046
AcuI CTGAAG 6 cut(s) 135, 1319, 2102, 2204, 3210, 3641
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
AfaI GTAC 6 cut(s) 536, 758, 922, 2261, 3482, 3757
AfiI CCNNNNNNNGG 2 cut(s) 2619, 2716
AflII CTTAAG 2 cut(s) 131, 3124
AflIII ACRYGT 1 cut(s) 2836
AgeI ACCGGT 1 cut(s) 652
AhdI GACNNNNNGTC 1 cut(s) 282
AhlI ACTAGT 1 cut(s) 2788
AjnI CCWGG 3 cut(s) 526, 910, 2710
Alw21I GWGCWC 3 cut(s) 1309, 2284, 3075
Alw26I GTCTC 9 cut(s) 285, 469, 611, 641, 963, 1283, 1387, 2818, 3439
AlwI GGATC 4 cut(s) 1074, 1470, 3139, 3543
AlwNI CAGNNNCTG 3 cut(s) 113, 911, 3764
Ama87I CYCGRG 1 cut(s) 1998
ApeKI GCWGC 7 cut(s) 1634, 1784, 1853, 2204, 2323, 2393, 3277
ApoI RAATTY 8 cut(s) 49, 185, 944, 1122, 1255, 1588, 1919, 2046
AseI ATTAAT 1 cut(s) 3392
AsiGI ACCGGT 1 cut(s) 652
Asp700I GAANNNNTTC 2 cut(s) 2365, 2733
AspLEI GCGC 1 cut(s) 2017
AspS9I GGNCC 5 cut(s) 339, 1227, 2077, 2645, 3775
AsuHPI GGTGA 3 cut(s) 431, 1752, 2198
AsuII TTCGAA 1 cut(s) 858
AvaI CYCGRG 1 cut(s) 1998
AvaII GGWCC 5 cut(s) 339, 1227, 2077, 2645, 3775
BaeGI GKGCMC 1 cut(s) 3267
BaeI ACNNNNGTAYC 2 cut(s) 2078, 2111
BanII GRGCYC 4 cut(s) 1309, 2284, 3558, 3771
BarI GAAGNNNNNNTAC 4 cut(s) 234, 266, 396, 428
BbrPI CACGTG 1 cut(s) 635
BbsI GAAGAC 4 cut(s) 984, 1505, 3621, 3700
Bbv12I GWGCWC 3 cut(s) 1309, 2284, 3075
BbvI GCAGC 7 cut(s) 1646, 1796, 1840, 2216, 2335, 2380, 3264
BccI CCATC 6 cut(s) 1669, 1958, 2489, 2493, 2842, 3002
BceAI ACGGC 2 cut(s) 1491, 1539
BcgI CGANNNNNNTGC 4 cut(s) 108, 142, 1075, 1109
BciT130I CCWGG 3 cut(s) 528, 912, 2712
BciVI GTATCC 1 cut(s) 2476
BclI TGATCA 2 cut(s) 1825, 2188
BcoDI GTCTC 9 cut(s) 285, 469, 611, 641, 963, 1283, 1387, 2818, 3439
BcuI ACTAGT 1 cut(s) 2788
BfaI CTAG 8 cut(s) 71, 722, 972, 1190, 1299, 1485, 2517, 2789
BfmI CTRYAG 3 cut(s) 449, 887, 1782
BfrI CTTAAG 2 cut(s) 131, 3124
BfuAI ACCTGC 1 cut(s) 894
BfuI GTATCC 1 cut(s) 2476
BlpI GCTNAGC 1 cut(s) 155
Bme1390I CCNGG 3 cut(s) 528, 912, 2712
Bme18I GGWCC 5 cut(s) 339, 1227, 2077, 2645, 3775
BmeRI GACNNNNNGTC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 1998
BmgT120I GGNCC 5 cut(s) 339, 1227, 2077, 2645, 3775
BmiI GGNNCC 4 cut(s) 340, 1228, 2007, 3777
BmrFI CCNGG 3 cut(s) 528, 912, 2712
BpiI GAAGAC 4 cut(s) 984, 1505, 3621, 3700
BpmI CTGGAG 1 cut(s) 3463
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 5 cut(s) 1307, 1583, 1706, 3330, 3487
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 635
BsaHI GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 4 cut(s) 94, 1491, 2090, 2710
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 4 cut(s) 2688, 2718, 3105, 3135
Bsc4I CCNNNNNNNGG 2 cut(s) 2619, 2716
Bse118I RCCGGY 1 cut(s) 652
Bse1I ACTGG 4 cut(s) 2238, 2465, 3183, 3228
Bse3DI GCAATG 6 cut(s) 149, 437, 489, 2052, 2613, 3606
BseBI CCWGG 3 cut(s) 528, 912, 2712
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 4 cut(s) 94, 1491, 2090, 2710
BseGI GGATG 8 cut(s) 90, 253, 1332, 1718, 2504, 2853, 3371, 3641
BseLI CCNNNNNNNGG 2 cut(s) 2619, 2716
BseMI GCAATG 6 cut(s) 149, 437, 489, 2052, 2613, 3606
BseNI ACTGG 4 cut(s) 2238, 2465, 3183, 3228
BseRI GAGGAG 7 cut(s) 181, 803, 1264, 1508, 1580, 2427, 3451
BseSI GKGCMC 1 cut(s) 3267
BseXI GCAGC 7 cut(s) 1646, 1796, 1840, 2216, 2335, 2380, 3264
BseYI CCCAGC 1 cut(s) 3764
BshTI ACCGGT 1 cut(s) 652
BshVI ATCGAT 1 cut(s) 231
BsiHKAI GWGCWC 3 cut(s) 1309, 2284, 3075
BsiHKCI CYCGRG 1 cut(s) 1998
BsiSI CCGG 2 cut(s) 470, 653
BslFI GGGAC 2 cut(s) 352, 3074
BslI CCNNNNNNNGG 2 cut(s) 2619, 2716
BsmAI GTCTC 9 cut(s) 285, 469, 611, 641, 963, 1283, 1387, 2818, 3439
BsmBI CGTCTC 3 cut(s) 285, 1283, 2818
BsmFI GGGAC 2 cut(s) 352, 3074
BsmI GAATGC 5 cut(s) 266, 309, 1369, 2477, 3230
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 1998
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 6 cut(s) 1309, 2284, 3075, 3267, 3558, 3771
Bsp1407I TGTACA 2 cut(s) 534, 3480
Bsp143I GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
Bsp1720I GCTNAGC 1 cut(s) 155
BspACI CCGC 5 cut(s) 629, 692, 1778, 3645, 3753
BspDI ATCGAT 1 cut(s) 231
BspHI TCATGA 1 cut(s) 2071
BspLI GGNNCC 4 cut(s) 340, 1228, 2007, 3777
BspMAI CTGCAG 3 cut(s) 453, 891, 1786
BspMI ACCTGC 1 cut(s) 894
BspPI GGATC 4 cut(s) 1074, 1470, 3139, 3543
BspQI GCTCTTC 2 cut(s) 1297, 2064
BspT104I TTCGAA 1 cut(s) 858
BspTI CTTAAG 2 cut(s) 131, 3124
BspTNI GGTCTC 1 cut(s) 469
BsrDI GCAATG 6 cut(s) 149, 437, 489, 2052, 2613, 3606
BsrFI RCCGGY 1 cut(s) 652
BsrGI TGTACA 2 cut(s) 534, 3480
BsrI ACTGG 4 cut(s) 2238, 2465, 3183, 3228
BssAI RCCGGY 1 cut(s) 652
BssECI CCNNGG 4 cut(s) 94, 1491, 2090, 2710
BssMI GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
BssNAI GTATAC 1 cut(s) 3383
BssNI GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
BssT1I CCWWGG 1 cut(s) 2090
Bst1107I GTATAC 1 cut(s) 3383
Bst2UI CCWGG 3 cut(s) 528, 912, 2712
Bst4CI ACNGT 7 cut(s) 367, 773, 1109, 1504, 2168, 2929, 3085
Bst6I CTCTTC 5 cut(s) 543, 1297, 2064, 3323, 3660
BstACI GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
BstAFI CTTAAG 2 cut(s) 131, 3124
BstAPI GCANNNNNTGC 2 cut(s) 448, 2023
BstAUI TGTACA 2 cut(s) 534, 3480
BstBAI YACGTR 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 858
BstC8I GCNNGC 6 cut(s) 318, 1442, 1782, 1955, 2477, 2987
BstF5I GGATG 8 cut(s) 90, 253, 1332, 1718, 2504, 2853, 3371, 3641
BstHHI GCGC 1 cut(s) 2017
BstKTI GATC 9 cut(s) 555, 1082, 1465, 1539, 1828, 2191, 3134, 3246, 3538
BstMAI GTCTC 9 cut(s) 285, 469, 611, 641, 963, 1283, 1387, 2818, 3439
BstMBI GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
BstMWI GCNNNNNNNGC 9 cut(s) 313, 322, 448, 698, 1049, 2012, 2023, 2279, 2963
BstNI CCWGG 3 cut(s) 528, 912, 2712
BstNSI RCATGY 6 cut(s) 1444, 1957, 2604, 2673, 2840, 3093
BstSCI CCNGG 3 cut(s) 526, 910, 2710
BstSFI CTRYAG 3 cut(s) 449, 887, 1782
BstSLI GKGCMC 1 cut(s) 3267
BstV1I GCAGC 7 cut(s) 1646, 1796, 1840, 2216, 2335, 2380, 3264
BstV2I GAAGAC 4 cut(s) 984, 1505, 3621, 3700
BstX2I RGATCY 2 cut(s) 1079, 3535
BstYI RGATCY 2 cut(s) 1079, 3535
BstZ17I GTATAC 1 cut(s) 3383
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 2476
BsuTUI ATCGAT 1 cut(s) 231
BtsCI GGATG 8 cut(s) 90, 253, 1332, 1718, 2504, 2853, 3371, 3641
BtsI GCAGTG 3 cut(s) 1815, 2388, 2956
BtsIMutI CAGTG 7 cut(s) 778, 1815, 2164, 2231, 2388, 2956, 3477
BveI ACCTGC 1 cut(s) 894
Cac8I GCNNGC 6 cut(s) 318, 1442, 1782, 1955, 2477, 2987
CaiI CAGNNNCTG 3 cut(s) 113, 911, 3764
CciI TCATGA 1 cut(s) 2071
CfoI GCGC 1 cut(s) 2017
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 5 cut(s) 339, 1227, 2077, 2645, 3775
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 4 cut(s) 88, 1300, 1503, 1783
Csp6I GTAC 6 cut(s) 535, 757, 921, 2260, 3481, 3756
CspAI ACCGGT 1 cut(s) 652
CviQI GTAC 6 cut(s) 535, 757, 921, 2260, 3481, 3756
DpnI GATC 9 cut(s) 554, 1081, 1464, 1538, 1827, 2190, 3133, 3245, 3537
DpnII GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
DraI TTTAAA 2 cut(s) 1587, 2658
DrdI GACNNNNNNGTC 1 cut(s) 2150
DriI GACNNNNNGTC 1 cut(s) 282
DseDI GACNNNNNNGTC 1 cut(s) 2150
Eam1104I CTCTTC 5 cut(s) 543, 1297, 2064, 3323, 3660
Eam1105I GACNNNNNGTC 1 cut(s) 282
EarI CTCTTC 5 cut(s) 543, 1297, 2064, 3323, 3660
Ecl136II GAGCTC 2 cut(s) 1307, 2282
Eco130I CCWWGG 1 cut(s) 2090
Eco24I GRGCYC 4 cut(s) 1309, 2284, 3558, 3771
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 5 cut(s) 339, 1227, 2077, 2645, 3775
Eco53kI GAGCTC 2 cut(s) 1307, 2282
Eco57I CTGAAG 6 cut(s) 135, 1319, 2102, 2204, 3210, 3641
Eco72I CACGTG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 1998
EcoICRI GAGCTC 2 cut(s) 1307, 2282
EcoO109I RGGNCCY 1 cut(s) 2077
EcoRI GAATTC 4 cut(s) 49, 185, 944, 1919
EcoRII CCWGG 3 cut(s) 526, 910, 2710
EcoT14I CCWWGG 1 cut(s) 2090
EcoT22I ATGCAT 6 cut(s) 146, 1174, 1955, 2753, 2940, 3371
EcoT38I GRGCYC 4 cut(s) 1309, 2284, 3558, 3771
ErhI CCWWGG 1 cut(s) 2090
Esp3I CGTCTC 3 cut(s) 285, 1283, 2818
FalI AAGNNNNNCTT 6 cut(s) 2589, 2621, 2667, 2699, 2718, 2750
FaqI GGGAC 2 cut(s) 352, 3074
FauNDI CATATG 2 cut(s) 2436, 3511
FbaI TGATCA 2 cut(s) 1825, 2188
FblI GTMKAC 2 cut(s) 87, 3382
FokI GGATG 8 cut(s) 77, 240, 1339, 1705, 2511, 2860, 3378, 3628
FriOI GRGCYC 4 cut(s) 1309, 2284, 3558, 3771
FspBI CTAG 8 cut(s) 71, 722, 972, 1190, 1299, 1485, 2517, 2789
FspI TGCGCA 1 cut(s) 2016
GlaI GCGC 1 cut(s) 2016
GsaI CCCAGC 1 cut(s) 3768
GsuI CTGGAG 1 cut(s) 3463
HapII CCGG 2 cut(s) 470, 653
HgaI GACGC 4 cut(s) 88, 1300, 1503, 1783
HhaI GCGC 1 cut(s) 2017
Hin1I GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
Hin6I GCGC 1 cut(s) 2015
HinP1I GCGC 1 cut(s) 2015
HindIII AAGCTT 2 cut(s) 243, 1050
HpaII CCGG 2 cut(s) 470, 653
HphI GGTGA 3 cut(s) 431, 1752, 2198
Hpy166II GTNNAC 8 cut(s) 88, 435, 535, 1473, 1883, 2558, 3383, 3481
Hpy8I GTNNAC 8 cut(s) 88, 435, 535, 1473, 1883, 2558, 3383, 3481
Hpy99I CGWCG 2 cut(s) 1519, 3436
HpyCH4III ACNGT 7 cut(s) 367, 773, 1109, 1504, 2168, 2929, 3085
HpyCH4IV ACGT 6 cut(s) 84, 278, 634, 2336, 2546, 2827
HpyF10VI GCNNNNNNNGC 9 cut(s) 313, 322, 448, 698, 1049, 2012, 2023, 2279, 2963
HpySE526I ACGT 6 cut(s) 84, 278, 634, 2336, 2546, 2827
Hsp92I GRCGYC 5 cut(s) 99, 278, 1514, 1775, 2827
HspAI GCGC 1 cut(s) 2015
Ksp22I TGATCA 2 cut(s) 1825, 2188
Kzo9I GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
LguI GCTCTTC 2 cut(s) 1297, 2064
LmnI GCTCC 6 cut(s) 1312, 2011, 2719, 2954, 3070, 3438
Lsp1109I GCAGC 7 cut(s) 1646, 1796, 1840, 2216, 2335, 2380, 3264
MaeI CTAG 8 cut(s) 71, 722, 972, 1190, 1299, 1485, 2517, 2789
MaeII ACGT 6 cut(s) 84, 278, 634, 2336, 2546, 2827
MaeIII GTNAC 9 cut(s) 881, 1390, 1742, 2152, 2296, 2430, 2839, 2959, 3085
MalI GATC 9 cut(s) 554, 1081, 1464, 1538, 1827, 2190, 3133, 3245, 3537
MboI GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
MflI RGATCY 2 cut(s) 1079, 3535
MhlI GDGCHC 6 cut(s) 1309, 2284, 3075, 3267, 3558, 3771
MlyI GAGTC 5 cut(s) 613, 936, 965, 1690, 2825
MmeI TCCRAC 2 cut(s) 1334, 1691
Mph1103I ATGCAT 6 cut(s) 146, 1174, 1955, 2753, 2940, 3371
MroXI GAANNNNTTC 2 cut(s) 2365, 2733
MslI CAYNNNNRTG 1 cut(s) 637
MspA1I CMGCKG 1 cut(s) 1468
MspCI CTTAAG 2 cut(s) 131, 3124
MspI CCGG 2 cut(s) 470, 653
MspR9I CCNGG 3 cut(s) 528, 912, 2712
Mva1269I GAATGC 5 cut(s) 266, 309, 1369, 2477, 3230
MvaI CCWGG 3 cut(s) 528, 912, 2712
MwoI GCNNNNNNNGC 9 cut(s) 313, 322, 448, 698, 1049, 2012, 2023, 2279, 2963
NdeI CATATG 2 cut(s) 2436, 3511
NdeII GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
NlaIV GGNNCC 4 cut(s) 340, 1228, 2007, 3777
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 3 cut(s) 2152, 2430, 2839
NsbI TGCGCA 1 cut(s) 2016
NsiI ATGCAT 6 cut(s) 146, 1174, 1955, 2753, 2940, 3371
NspI RCATGY 6 cut(s) 1444, 1957, 2604, 2673, 2840, 3093
NspV TTCGAA 1 cut(s) 858
PaeI GCATGC 2 cut(s) 1444, 1957
PaeR7I CTCGAG 1 cut(s) 1998
PagI TCATGA 1 cut(s) 2071
PciI ACATGT 1 cut(s) 2836
PciSI GCTCTTC 2 cut(s) 1297, 2064
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 5 cut(s) 266, 309, 1369, 2477, 3230
PdmI GAANNNNTTC 2 cut(s) 2365, 2733
PflMI CCANNNNNTGG 1 cut(s) 2619
PinAI ACCGGT 1 cut(s) 652
PleI GAGTC 5 cut(s) 613, 935, 964, 1689, 2824
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
PpsI GAGTC 5 cut(s) 613, 935, 964, 1689, 2824
Ppu21I YACGTR 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 2077
PscI ACATGT 1 cut(s) 2836
PshBI ATTAAT 1 cut(s) 3392
PsiI TTATAA 2 cut(s) 1056, 3254
Psp124BI GAGCTC 2 cut(s) 1309, 2284
Psp1406I AACGTT 1 cut(s) 2336
Psp5II RGGWCCY 1 cut(s) 2077
Psp6I CCWGG 3 cut(s) 526, 910, 2710
PspCI CACGTG 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 3764
PspGI CCWGG 3 cut(s) 526, 910, 2710
PspN4I GGNNCC 4 cut(s) 340, 1228, 2007, 3777
PspPI GGNCC 5 cut(s) 339, 1227, 2077, 2645, 3775
PspPPI RGGWCCY 1 cut(s) 2077
PspXI VCTCGAGB 1 cut(s) 1998
PsrI GAACNNNNNNTAC 2 cut(s) 2243, 2275
PstI CTGCAG 3 cut(s) 453, 891, 1786
PstNI CAGNNNCTG 3 cut(s) 113, 911, 3764
PsuI RGATCY 2 cut(s) 1079, 3535
PvuII CAGCTG 1 cut(s) 1468
RsaI GTAC 6 cut(s) 536, 758, 922, 2261, 3482, 3757
RsaNI GTAC 6 cut(s) 535, 757, 921, 2260, 3481, 3756
RseI CAYNNNNRTG 1 cut(s) 637
SacI GAGCTC 2 cut(s) 1309, 2284
SapI GCTCTTC 2 cut(s) 1297, 2064
Sau3AI GATC 9 cut(s) 552, 1079, 1462, 1536, 1825, 2188, 3131, 3243, 3535
Sau96I GGNCC 5 cut(s) 339, 1227, 2077, 2645, 3775
SbfI CCTGCAGG 1 cut(s) 891
SchI GAGTC 5 cut(s) 613, 936, 965, 1690, 2825
ScrFI CCNGG 3 cut(s) 528, 912, 2712
SdaI CCTGCAGG 1 cut(s) 891
SduI GDGCHC 6 cut(s) 1309, 2284, 3075, 3267, 3558, 3771
SfcI CTRYAG 3 cut(s) 449, 887, 1782
Sfr274I CTCGAG 1 cut(s) 1998
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 5 cut(s) 339, 1227, 2077, 2645, 3775
SlaI CTCGAG 1 cut(s) 1998
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 8 cut(s) 131, 1286, 1562, 1685, 1998, 3124, 3345, 3502
SmoI CTYRAG 8 cut(s) 131, 1286, 1562, 1685, 1998, 3124, 3345, 3502
SpeI ACTAGT 1 cut(s) 2788
SphI GCATGC 2 cut(s) 1444, 1957
Sse8387I CCTGCAGG 1 cut(s) 891
SsiI CCGC 5 cut(s) 629, 692, 1778, 3645, 3753
SspI AATATT 1 cut(s) 687
SspMI CTAG 8 cut(s) 71, 722, 972, 1190, 1299, 1485, 2517, 2789
SstI GAGCTC 2 cut(s) 1309, 2284
StyD4I CCNGG 3 cut(s) 526, 910, 2710
StyI CCWWGG 1 cut(s) 2090
TaaI ACNGT 7 cut(s) 367, 773, 1109, 1504, 2168, 2929, 3085
TaiI ACGT 6 cut(s) 87, 281, 637, 2339, 2549, 2830
TaqI TCGA 8 cut(s) 26, 65, 231, 240, 858, 1517, 1999, 3634
TatI WGTACW 4 cut(s) 534, 920, 2259, 3480
TauI GCSGC 3 cut(s) 631, 695, 1780
TscAI CASTG 7 cut(s) 778, 1822, 2171, 2238, 2395, 2956, 3477
TseFI GTSAC 3 cut(s) 2152, 2430, 2839
TseI GCWGC 7 cut(s) 1634, 1784, 1853, 2204, 2323, 2393, 3277
Tsp45I GTSAC 3 cut(s) 2152, 2430, 2839
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 7 cut(s) 778, 1822, 2171, 2238, 2395, 2956, 3477
Van91I CCANNNNNTGG 1 cut(s) 2619
Vha464I CTTAAG 2 cut(s) 131, 3124
VpaK11BI GGWCC 5 cut(s) 339, 1227, 2077, 2645, 3775
VspI ATTAAT 1 cut(s) 3392
XapI RAATTY 8 cut(s) 49, 185, 944, 1122, 1255, 1588, 1919, 2046
XbaI TCTAGA 1 cut(s) 1298
XceI RCATGY 6 cut(s) 1444, 1957, 2604, 2673, 2840, 3093
XhoI CTCGAG 1 cut(s) 1998
XmiI GTMKAC 2 cut(s) 87, 3382
XmnI GAANNNNTTC 2 cut(s) 2365, 2733
XspI CTAG 8 cut(s) 71, 722, 972, 1190, 1299, 1485, 2517, 2789
ZraI GACGTC 2 cut(s) 279, 2828
Zsp2I ATGCAT 6 cut(s) 146, 1174, 1955, 2753, 2940, 3371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.