Rroxscaffold_5G00364690

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010154
Physical Location & Seq
Forward (+)
45882750 .. 45889796
7047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364690.1

Sequence Viewer

Length: 3984 bp
ATGAACTTGCTTGGTGACTCGGTGGAAGCTGTAGCTAGCTGTTCTACTACTGCTTTGGTTTCGTCTCATAATCAAGAGAGATTAGACCTCAAGTCCATTCGGGCGGTTGTCATTTCAAGCAATCAATGCATTCTCAAACTCATAGCAAATGACAAAACTAGAAACTCTATAAGGGTGAGATGTATCTCCAAGCTTCAAAATCAGAAACAGGATTTCTTCGAATTCTCTGACCAGTCTGTCATATCAAATCTTTACTGGGGTATTGACAGCATAGAAGCTGCAATTACCGCTGAATGGCCAGAAGAGAAAGCCGCTCAGCTGAGGAAGGCAGAGCAGATGCTACAAGTGCCCGCGCTACTCGATGAGGATGGAGTCACAGCTGGAATTCCGAACAGTTATTTGGTGTGTTGCTCATACTTTTACCTCTCTGTTGTTCGAAAACTCCAAGAGGATGAGTGGCAGGTTGCACTGCATTTCCTACAGGCCGTGCTGGTCTCCCCGAGACTTGTTCAGACAGAATTTGCACATGACCTTTATGAAAGTCTTTTCCCGACTTGCGGCGGTTCTGAAAGACAAGAAATAAGAGCGAGTAAGAGCTTGGAATCAATTGACAAGGATGAGGCGATAAGGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGCGCGACTCCTCAGGGTCAAGGTGGAGGATACAGAGGCATTTTGTCCCCTGACAAAGAGTCACTATATTCTCTGCATGGGAAATCCACTAGGTCGGACTGTTCAAATAATATTGAGCATGACCATAACTTGCACACTCAATGGAATTATGGAAAAGTGCACCCTTTTGATCCACAAGAAGATATTGTCATCGAAGATGAACTGAAACAATCCATCCACATCTCAGAATTTGAAGAATATGGGAAATCCAAAAATGATTTGAATCAAGCAACAGAGTTTCATGTTAAAACTAGAGAGCTCCAGAGGAATTCAAGTATTAAAAGACTCCAAGATTTGCTGAATGACTCTCAATCAGACACACCAACTTCGGTGGATTCATGCTCTGATTATTCAGATTATGACATTGAGTCAGAGGTCATTGATGGTGGTGAGTGCTCAACCAGAGATGCAAGAATAGGTGCAGATTTTCCCAAGAAACTGCAGGCTACTAGCTCCACATCAGACCCAAAGTACGAAGCATTATCTTTTTCACAAGTCTGTCAGGATCCAGTCCCTAATGAAGTCATTCAAGTAAATAACTCTATGATTCTTTCAAGCAGATTCACAAATTCCATCAATGGTTTATTAACTATTTCAGAGCATAGAGATAAAAGATCAAAGACCCTTCAGAATTTATATGTACAAAAGGAGTATGCATCTCAGCGGAACTATAGGATCAACCAAAGGGATCACCAAAGAAGTGTTGCAAGGAAAAAACATAGTTCTCGTAGCCAGAAAAGCTCTATTGAACTTCGCTTACATTCCACAAAAGATTCGAAAAGTGAACTACTGAGTATTACTGAAAAAGCAATTTCAAAACTATTTCAGTGGGAAGGATTTGGAAAATGGGATGAAGATTATGCTGTAGAAGTTACAACAATATATCAAATACTATGCAACAAAAAAGGAGAGAAATGTGCTGTCTTGAAAGACATGATATTGGATCAACTGCTCATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTGATAAGGGTATCAGTATCGATACTAACTACTATTGTTGCAGCTAATAAGTCAGTTATCAAGGACATAAAGAAGAAAGGTCTGCAGTTATCTGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCATCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTGTTGGGTGTTGTGTGCTCTCCAAACAGTTATAAGGGTAGGCCAGCATCAGTGCCAACACCTCTGACAGCATCACTAATGATTATTGGAGTGTTAGTATCTTCATTTGACCATGCCACAAATAATGTGCACTTGGCTGAAATCAGTTATCCCAATGTGCTGCATGGGCTCCTAGATGTTGCAAGAGATAGTAACATTGAAGAATTGATCTCCTGGGCCACTATTCTTGTCAAATGCGTACAATACGATGGAAACTGCAGAAGATATATATCAAGATTAGCTCCAATGGCTCCATTTTCCCGTCTCCTAGAATGTAAGATGAAGCATGCCCGGTGCATAGCGCTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCGGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACGAAGATTATGAACTCACTCATGCTTTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCATTTAGATACACTAGATAACTCCTCCTGTAAGAGTGCATTTACAGAAGAAGCTATGCAGGTTCTCCTCAAGTTGGTCGCATCTGAAGAAAGTTCCACTACACAGCACTTATCTGCATTCATCTTATCAAATCTCGGTGGAACATATTCATGGTCAGGGGAACCATATACAGTAGCATGGTTGGTTAAAAAGGCAGGTGTGACTTCCTCGTATCGACGGAATATGATAAAAACTATCCATTGGTTGGATGATTGCTTAGAGGATTCTGGCACGGACTCATGGTGCAGCAAAATTTCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTGATTCTTTAGTGAAGGGTCTAAAGAGCACAACAAGGAAGGTTTCTAGGGACTGTCTAATAGCCATAGCATGGCTTGGGTTCGAAATTGCAAAGAGTCCAGATATCATCAGATATTCTGCCTGTGAGATCTTACTTAGTGGAGTTGAGCAATTTTTGCATCCTGGATTGGAGCTAGAAGAACGAGTTCTAGCATGTCTCTGCATATATAACTATGCTTCTGGTAAAGGGATGAAAAAGTTGATTCATTTCTCGGAAGGAGTGAGAGAATCACTAAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGCTCACAGCGAATTTCTTGTGTTCATACCCAAATTCTCGAGGTTGGAGTCAATTACAGTGGAGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGCTACTCAGATGGTTCTGTGAAGGTCTGGAACATAAAAGGTCAGTCAGCCACACTCGTATGGGACATGAAGGAGCACAAAAAGGCAGTTACATGCTTTTCACTACTTGAATCCAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTATCAGGGTCTGGCAAGTGGTCCGCAAAAAACTGGAGTGCATTGAGGTGATAGAAACCAAGGAACCAATTCGGCATCTGAATACATGCGGGGACATGATATTTGCAATTACCCGTGGCCATGGGATTAAGGTGATTGATGCATCAAGAAAGGTCAAAGATAATTGCACGAGTAAAAAGGTAAAGTGTATGGCGGTGGTTCAGGGGAAGATTTATGCAGGTTGCAAGGATTCAAGCATACAGGAGTTGTCTACAACAAGCAATCGGGCACAAGAGATCAAAGCAGCAGCAAAATTTTGGAAGCTGAAACGGAGACCCATAAATGCAGTTGTCATATACAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGCAAGGTTCAAACTTGAAGGAATGGAAAAGACACGGCAAACCCCAAATGTCACTGAAATCCGGGAAAAGAGAGAAGGTGACGGCAATGGGAATAACAGAAGACTTCATATACCTCAACTGCAAATCATCAACAAACAGCATCCAGATATGGCTAAGAGGGACACAGCAGAAAGTGGGCAGAATTTCAGCAGGAAGCAGAATTACGAGCATCCTCACAGCCAACGACATTATCCTGTGTGGTACCGAAACCGGGCTAATCAAGGGATGGATCCCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1327

Amino Acids

149.04

Weight (kDa)

8.58

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 33 - 184 2e-28 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 503 - 664 3.8e-47 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 668 - 1020 2.3e-56 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_TEP1_2nd PF25047 1051 - 1118 6.2e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 1060 - 1123 6.3e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1062 - 1302 1.7e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1063 - 1132 1.9e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1064 - 1137 5.7e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 669, 1938, 3314
AarI CACCTGC 1 cut(s) 2594
AasI GACNNNNNNGTC 1 cut(s) 236
Acc36I ACCTGC 4 cut(s) 451, 2458, 2594, 3542
Acc65I GGTACC 1 cut(s) 3944
AccB1I GGYRCC 1 cut(s) 3944
AccB7I CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
AccBSI CCGCTC 2 cut(s) 314, 3084
AccI GTMKAC 1 cut(s) 3584
AccII CGCG 2 cut(s) 353, 689
AclWI GGATC 9 cut(s) 848, 1223, 1236, 1406, 1419, 1674, 3328, 3967, 3980
AcoI YGGCCR 2 cut(s) 296, 3451
AcuI CTGAAG 2 cut(s) 1334, 2514
AfaI GTAC 4 cut(s) 1196, 1365, 2145, 3946
AfeI AGCGCT 1 cut(s) 2247
AfiI CCNNNNNNNGG 7 cut(s) 294, 557, 2389, 2482, 2653, 2808, 3954
AhdI GACNNNNNGTC 3 cut(s) 91, 742, 1090
AjnI CCWGG 4 cut(s) 2117, 2899, 3032, 3299
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
Alw21I GWGCWC 8 cut(s) 846, 984, 1121, 1816, 1925, 2037, 2768, 3264
Alw26I GTCTC 6 cut(s) 69, 496, 499, 2213, 2939, 3640
Alw44I GTGCAC 2 cut(s) 842, 2033
AlwI GGATC 9 cut(s) 848, 1223, 1236, 1406, 1419, 1674, 3328, 3967, 3980
AlwNI CAGNNNCTG 2 cut(s) 2311, 3345
Ama87I CYCGRG 2 cut(s) 499, 3119
Aor51HI AGCGCT 1 cut(s) 2247
AoxI GGCC 5 cut(s) 296, 483, 1946, 2121, 3451
ApaLI GTGCAC 2 cut(s) 842, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 3419, 3451
Asp700I GAANNNNTTC 5 cut(s) 1248, 2256, 2554, 2922, 3402
Asp718I GGTACC 1 cut(s) 3944
AspLEI GCGC 3 cut(s) 355, 689, 2248
AspS9I GGNCC 2 cut(s) 2121, 3355
AsuC2I CCSGG 3 cut(s) 2236, 3766, 3955
AsuHPI GGTGA 7 cut(s) 26, 187, 1124, 1406, 3394, 3478, 3793
AsuII TTCGAA 4 cut(s) 219, 436, 1499, 2820
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 2 cut(s) 499, 3119
AvaII GGWCC 1 cut(s) 3355
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 5 cut(s) 351, 846, 2037, 3157, 3604
BalI TGGCCA 2 cut(s) 298, 3453
BamHI GGATCC 2 cut(s) 1228, 3972
BanI GGYRCC 1 cut(s) 3944
BanII GRGCYC 2 cut(s) 984, 2076
BarI GAAGNNNNNNTAC 4 cut(s) 1675, 1707, 3889, 3921
BauI CACGAG 1 cut(s) 3502
BbsI GAAGAC 1 cut(s) 3810
Bbv12I GWGCWC 8 cut(s) 846, 984, 1121, 1816, 1925, 2037, 2768, 3264
BbvCI CCTCAGC 1 cut(s) 320
BceAI ACGGC 3 cut(s) 470, 3754, 3801
BciT130I CCWGG 4 cut(s) 2119, 2901, 3034, 3301
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 3 cut(s) 2236, 3766, 3955
BcoDI GTCTC 6 cut(s) 69, 496, 499, 2213, 2939, 3640
BfmI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1587, 1790, 1872, 2161, 3980
BfoI RGCGCY 1 cut(s) 2249
BfuAI ACCTGC 4 cut(s) 451, 2458, 2594, 3542
BfuI GTATCC 1 cut(s) 707
BglII AGATCT 1 cut(s) 2865
BlpI GCTNAGC 1 cut(s) 315
Bme1390I CCNGG 7 cut(s) 2119, 2236, 2901, 3034, 3301, 3766, 3955
Bme18I GGWCC 1 cut(s) 3355
BmeRI GACNNNNNGTC 3 cut(s) 91, 742, 1090
BmeT110I CYCGRG 2 cut(s) 499, 3119
BmgT120I GGNCC 2 cut(s) 2121, 3355
BmiI GGNNCC 7 cut(s) 1230, 2075, 2196, 2571, 3399, 3946, 3974
BmrFI CCNGG 7 cut(s) 2119, 2236, 2901, 3034, 3301, 3766, 3955
BmrI ACTGGG 1 cut(s) 265
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 1 cut(s) 265
BoxI GACNNNNGTC 1 cut(s) 3019
BpiI GAAGAC 1 cut(s) 3810
BpmI CTGGAG 2 cut(s) 968, 3389
Bpu10I CCTNAGC 1 cut(s) 320
Bpu1102I GCTNAGC 1 cut(s) 315
Bpu14I TTCGAA 4 cut(s) 219, 436, 1499, 2820
BpuEI CTTGAG 2 cut(s) 74, 2462
BpuMI CCSGG 3 cut(s) 2236, 3766, 3955
Bsa29I ATCGAT 1 cut(s) 1727
BsaBI GATNNNNATC 2 cut(s) 945, 2173
BsaI GGTCTC 2 cut(s) 499, 3640
BsaJI CCNNGG 6 cut(s) 2118, 2279, 3300, 3393, 3448, 3454
BsaXI ACNNNNNCTCC 2 cut(s) 1986, 2016
Bsc4I CCNNNNNNNGG 7 cut(s) 294, 557, 2389, 2482, 2653, 2808, 3954
Bse118I RCCGGY 1 cut(s) 1900
Bse1I ACTGG 5 cut(s) 232, 260, 1232, 3330, 3372
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 3795
Bse8I GATNNNNATC 2 cut(s) 945, 2173
BseBI CCWGG 4 cut(s) 2119, 2901, 3034, 3301
BseCI ATCGAT 1 cut(s) 1727
BseDI CCNNGG 6 cut(s) 2118, 2279, 3300, 3393, 3448, 3454
BseJI GATNNNNATC 2 cut(s) 945, 2173
BseLI CCNNNNNNNGG 7 cut(s) 294, 557, 2389, 2482, 2653, 2808, 3954
BseMI GCAATG 1 cut(s) 3795
BseMII CTCAG 7 cut(s) 311, 329, 710, 921, 1397, 1505, 3207
BseNI ACTGG 5 cut(s) 232, 260, 1232, 3330, 3372
BseRI GAGGAG 5 cut(s) 684, 1715, 2422, 2465, 3059
BseSI GKGCMC 5 cut(s) 351, 846, 2037, 3157, 3604
BsgI GTGCAG 2 cut(s) 1164, 2713
Bsh1236I CGCG 2 cut(s) 353, 689
BshFI GGCC 5 cut(s) 298, 485, 1948, 2123, 3453
BshNI GGYRCC 1 cut(s) 3944
BshVI ATCGAT 1 cut(s) 1727
BsiHKAI GWGCWC 8 cut(s) 846, 984, 1121, 1816, 1925, 2037, 2768, 3264
BsiHKCI CYCGRG 2 cut(s) 499, 3119
BsiSI CCGG 4 cut(s) 1901, 2236, 3765, 3954
BslFI GGGAC 7 cut(s) 715, 1220, 2801, 3263, 3317, 3440, 3877
BslI CCNNNNNNNGG 7 cut(s) 294, 557, 2389, 2482, 2653, 2808, 3954
BsmAI GTCTC 6 cut(s) 69, 496, 499, 2213, 2939, 3640
BsmBI CGTCTC 2 cut(s) 69, 2213
BsmFI GGGAC 7 cut(s) 715, 1220, 2801, 3263, 3317, 3440, 3877
BsmI GAATGC 3 cut(s) 129, 2274, 2525
BsnI GGCC 5 cut(s) 298, 485, 1948, 2123, 3453
Bso31I GGTCTC 2 cut(s) 499, 3640
BsoBI CYCGRG 2 cut(s) 499, 3119
Bsp119I TTCGAA 4 cut(s) 219, 436, 1499, 2820
Bsp1407I TGTACA 1 cut(s) 1363
Bsp1720I GCTNAGC 1 cut(s) 315
Bsp19I CCATGG 1 cut(s) 3454
BspANI GGCC 5 cut(s) 298, 485, 1948, 2123, 3453
BspCNI CTCAG 7 cut(s) 312, 328, 709, 920, 1396, 1506, 3206
BspDI ATCGAT 1 cut(s) 1727
BspFNI CGCG 2 cut(s) 353, 689
BspHI TCATGA 2 cut(s) 1831, 2260
BspLI GGNNCC 7 cut(s) 1230, 2075, 2196, 2571, 3399, 3946, 3974
BspMAI CTGCAG 3 cut(s) 1167, 1794, 2165
BspMI ACCTGC 4 cut(s) 451, 2458, 2594, 3542
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 9 cut(s) 848, 1223, 1236, 1406, 1419, 1674, 3328, 3967, 3980
BspT104I TTCGAA 4 cut(s) 219, 436, 1499, 2820
BspT107I GGYRCC 1 cut(s) 3944
BspTNI GGTCTC 2 cut(s) 499, 3640
BsrBI CCGCTC 2 cut(s) 314, 3084
BsrDI GCAATG 1 cut(s) 3795
BsrFI RCCGGY 1 cut(s) 1900
BsrGI TGTACA 1 cut(s) 1363
BsrI ACTGG 5 cut(s) 232, 260, 1232, 3330, 3372
BssAI RCCGGY 1 cut(s) 1900
BssECI CCNNGG 6 cut(s) 2118, 2279, 3300, 3393, 3448, 3454
BssSI CACGAG 1 cut(s) 3502
BssT1I CCWWGG 3 cut(s) 2279, 3393, 3454
Bst2BI CACGAG 1 cut(s) 3502
Bst2UI CCWGG 4 cut(s) 2119, 2901, 3034, 3301
Bst4CI ACNGT 8 cut(s) 395, 785, 1848, 1934, 2581, 2792, 3140, 3689
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 3 cut(s) 126, 2311, 2893
BstAUI TGTACA 1 cut(s) 1363
BstBI TTCGAA 4 cut(s) 219, 436, 1499, 2820
BstC8I GCNNGC 5 cut(s) 37, 351, 1167, 1950, 2232
BstDSI CCRYGG 2 cut(s) 3448, 3454
BstFNI CGCG 2 cut(s) 353, 689
BstH2I RGCGCY 1 cut(s) 2249
BstHHI GCGC 3 cut(s) 355, 689, 2248
BstMAI GTCTC 6 cut(s) 69, 496, 499, 2213, 2939, 3640
BstMWI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1461, 2071, 2192, 2311, 2893, 3152
BstNI CCWGG 4 cut(s) 2119, 2901, 3034, 3301
BstNSI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
BstPAI GACNNNNGTC 1 cut(s) 3019
BstSCI CCNGG 7 cut(s) 2117, 2234, 2899, 3032, 3299, 3764, 3953
BstSFI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1587, 1790, 1872, 2161, 3980
BstSLI GKGCMC 5 cut(s) 351, 846, 2037, 3157, 3604
BstUI CGCG 2 cut(s) 353, 689
BstV2I GAAGAC 1 cut(s) 3810
BstX2I RGATCY 3 cut(s) 1228, 2865, 3972
BstYI RGATCY 3 cut(s) 1228, 2865, 3972
Bsu15I ATCGAT 1 cut(s) 1727
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 5 cut(s) 298, 485, 1948, 2123, 3453
BsuTUI ATCGAT 1 cut(s) 1727
BtgI CCRYGG 2 cut(s) 3448, 3454
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 7 cut(s) 467, 1556, 1962, 3145, 3323, 3694, 3755
BveI ACCTGC 4 cut(s) 451, 2458, 2594, 3542
Cac8I GCNNGC 5 cut(s) 37, 351, 1167, 1950, 2232
CaiI CAGNNNCTG 2 cut(s) 2311, 3345
CciI TCATGA 2 cut(s) 1831, 2260
CfoI GCGC 3 cut(s) 355, 689, 2248
Cfr10I RCCGGY 1 cut(s) 1900
Cfr13I GGNCC 2 cut(s) 2121, 3355
ClaI ATCGAT 1 cut(s) 1727
CseI GACGC 1 cut(s) 3024
Csp6I GTAC 4 cut(s) 1195, 1364, 2144, 3945
CspCI CAANNNNNGTGG 6 cut(s) 1035, 1070, 2113, 2148, 3121, 3156
CviQI GTAC 4 cut(s) 1195, 1364, 2144, 3945
DrdI GACNNNNNNGTC 1 cut(s) 236
DriI GACNNNNNGTC 3 cut(s) 91, 742, 1090
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 2 cut(s) 296, 3451
Eam1104I CTCTTC 1 cut(s) 297
Eam1105I GACNNNNNGTC 3 cut(s) 91, 742, 1090
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 982
Eco130I CCWWGG 3 cut(s) 2279, 3393, 3454
Eco24I GRGCYC 2 cut(s) 984, 2076
Eco31I GGTCTC 2 cut(s) 499, 3640
Eco32I GATATC 1 cut(s) 2842
Eco47I GGWCC 1 cut(s) 3355
Eco47III AGCGCT 1 cut(s) 2247
Eco53kI GAGCTC 1 cut(s) 982
Eco57I CTGAAG 2 cut(s) 1334, 2514
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 499, 3119
EcoICRI GAGCTC 1 cut(s) 982
EcoRI GAATTC 3 cut(s) 221, 384, 991
EcoRII CCWGG 4 cut(s) 2117, 2899, 3032, 3299
EcoRV GATATC 1 cut(s) 2842
EcoT14I CCWWGG 3 cut(s) 2279, 3393, 3454
EcoT22I ATGCAT 3 cut(s) 131, 1381, 3478
EcoT38I GRGCYC 2 cut(s) 984, 2076
ErhI CCWWGG 3 cut(s) 2279, 3393, 3454
Esp3I CGTCTC 2 cut(s) 69, 2213
FalI AAGNNNNNCTT 4 cut(s) 3162, 3194, 3666, 3698
FaqI GGGAC 7 cut(s) 715, 1220, 2801, 3263, 3317, 3440, 3877
FauI CCCGC 2 cut(s) 358, 3416
FblI GTMKAC 1 cut(s) 3584
FriOI GRGCYC 2 cut(s) 984, 2076
GlaI GCGC 3 cut(s) 354, 688, 2247
GsuI CTGGAG 2 cut(s) 968, 3389
HaeII RGCGCY 1 cut(s) 2249
HaeIII GGCC 5 cut(s) 298, 485, 1948, 2123, 3453
HapII CCGG 4 cut(s) 1901, 2236, 3765, 3954
HgaI GACGC 1 cut(s) 3024
HhaI GCGC 3 cut(s) 355, 689, 2248
Hin6I GCGC 3 cut(s) 353, 687, 2246
HinP1I GCGC 3 cut(s) 353, 687, 2246
HindIII AAGCTT 1 cut(s) 191
HpaII CCGG 4 cut(s) 1901, 2236, 3765, 3954
HphI GGTGA 7 cut(s) 26, 187, 1124, 1406, 3394, 3478, 3793
Hpy166II GTNNAC 4 cut(s) 844, 1508, 2035, 3585
Hpy8I GTNNAC 4 cut(s) 844, 1508, 2035, 3585
Hpy99I CGWCG 1 cut(s) 2628
HpyCH4III ACNGT 8 cut(s) 395, 785, 1848, 1934, 2581, 2792, 3140, 3689
HpyF10VI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1461, 2071, 2192, 2311, 2893, 3152
HspAI GCGC 3 cut(s) 353, 687, 2246
KpnI GGTACC 1 cut(s) 3948
LmnI GCTCC 9 cut(s) 987, 1181, 2079, 2191, 2200, 2908, 3046, 3143, 3259
MbiI CCGCTC 2 cut(s) 314, 3084
MfeI CAATTG 3 cut(s) 606, 2366, 3699
MflI RGATCY 3 cut(s) 1228, 2865, 3972
MlsI TGGCCA 2 cut(s) 298, 3453
MluNI TGGCCA 2 cut(s) 298, 3453
MmeI TCCRAC 4 cut(s) 759, 2386, 2634, 3106
Mox20I TGGCCA 2 cut(s) 298, 3453
Mph1103I ATGCAT 3 cut(s) 131, 1381, 3478
MroXI GAANNNNTTC 5 cut(s) 1248, 2256, 2554, 2922, 3402
MscI TGGCCA 2 cut(s) 298, 3453
MseI TTAA 7 cut(s) 969, 1002, 1310, 1881, 2595, 2718, 3462
MslI CAYNNNNRTG 3 cut(s) 2557, 3244, 3380
Msp20I TGGCCA 2 cut(s) 298, 3453
MspA1I CMGCKG 4 cut(s) 290, 319, 380, 1387
MspI CCGG 4 cut(s) 1901, 2236, 3765, 3954
MspR9I CCNGG 7 cut(s) 2119, 2236, 2901, 3034, 3301, 3766, 3955
MunI CAATTG 3 cut(s) 606, 2366, 3699
Mva1269I GAATGC 3 cut(s) 129, 2274, 2525
MvaI CCWGG 4 cut(s) 2119, 2901, 3034, 3301
MvnI CGCG 2 cut(s) 353, 689
MwoI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1461, 2071, 2192, 2311, 2893, 3152
NciI CCSGG 3 cut(s) 2236, 3766, 3955
NcoI CCATGG 1 cut(s) 3454
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 7 cut(s) 1230, 2075, 2196, 2571, 3399, 3946, 3974
NmuCI GTSAC 6 cut(s) 14, 373, 744, 2608, 3753, 3781
NsiI ATGCAT 3 cut(s) 131, 1381, 3478
NspI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
NspV TTCGAA 4 cut(s) 219, 436, 1499, 2820
PaeI GCATGC 1 cut(s) 2234
PaeR7I CTCGAG 1 cut(s) 3119
PagI TCATGA 2 cut(s) 1831, 2260
PaqCI CACCTGC 1 cut(s) 2594
PctI GAATGC 3 cut(s) 129, 2274, 2525
PdmI GAANNNNTTC 5 cut(s) 1248, 2256, 2554, 2922, 3402
PflMI CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
PfoI TCCNGGA 2 cut(s) 2899, 3764
PshAI GACNNNNGTC 1 cut(s) 3019
PsiI TTATAA 3 cut(s) 669, 1938, 3314
Psp124BI GAGCTC 1 cut(s) 984
Psp6I CCWGG 4 cut(s) 2117, 2899, 3032, 3299
PspGI CCWGG 4 cut(s) 2117, 2899, 3032, 3299
PspN4I GGNNCC 7 cut(s) 1230, 2075, 2196, 2571, 3399, 3946, 3974
PspPI GGNCC 2 cut(s) 2121, 3355
PsrI GAACNNNNNNTAC 2 cut(s) 1464, 1496
PstI CTGCAG 3 cut(s) 1167, 1794, 2165
PstNI CAGNNNCTG 2 cut(s) 2311, 3345
PsuI RGATCY 3 cut(s) 1228, 2865, 3972
PvuII CAGCTG 2 cut(s) 319, 380
RsaI GTAC 4 cut(s) 1196, 1365, 2145, 3946
RsaNI GTAC 4 cut(s) 1195, 1364, 2144, 3945
RseI CAYNNNNRTG 3 cut(s) 2557, 3244, 3380
SacI GAGCTC 1 cut(s) 984
SaqAI TTAA 7 cut(s) 969, 1002, 1310, 1881, 2595, 2718, 3462
Sau96I GGNCC 2 cut(s) 2121, 3355
ScrFI CCNGG 7 cut(s) 2119, 2236, 2901, 3034, 3301, 3766, 3955
SfcI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1587, 1790, 1872, 2161, 3980
Sfr274I CTCGAG 1 cut(s) 3119
SfuI TTCGAA 4 cut(s) 219, 436, 1499, 2820
SinI GGWCC 1 cut(s) 3355
SlaI CTCGAG 1 cut(s) 3119
SmiMI CAYNNNNRTG 3 cut(s) 2557, 3244, 3380
SmlI CTYRAG 3 cut(s) 89, 2477, 3119
SmoI CTYRAG 3 cut(s) 89, 2477, 3119
SphI GCATGC 1 cut(s) 2234
SspI AATATT 1 cut(s) 796
SstI GAGCTC 1 cut(s) 984
StyD4I CCNGG 7 cut(s) 2117, 2234, 2899, 3032, 3299, 3764, 3953
StyI CCWWGG 3 cut(s) 2279, 3393, 3454
TaaI ACNGT 8 cut(s) 395, 785, 1848, 1934, 2581, 2792, 3140, 3689
TaqI TCGA 9 cut(s) 219, 360, 436, 876, 1499, 1727, 2623, 2820, 3120
TatI WGTACW 1 cut(s) 1363
TauI GCSGC 2 cut(s) 314, 561
Tru1I TTAA 7 cut(s) 969, 1002, 1310, 1881, 2595, 2718, 3462
Tru9I TTAA 7 cut(s) 969, 1002, 1310, 1881, 2595, 2718, 3462
TscAI CASTG 7 cut(s) 474, 1556, 1962, 3145, 3330, 3694, 3762
TseFI GTSAC 6 cut(s) 14, 373, 744, 2608, 3753, 3781
Tsp45I GTSAC 6 cut(s) 14, 373, 744, 2608, 3753, 3781
TspGWI ACGGA 3 cut(s) 2641, 2696, 3658
TspRI CASTG 7 cut(s) 474, 1556, 1962, 3145, 3330, 3694, 3762
Van91I CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
VneI GTGCAC 2 cut(s) 842, 2033
VpaK11BI GGWCC 1 cut(s) 3355
XceI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
XhoI CTCGAG 1 cut(s) 3119
XmiI GTMKAC 1 cut(s) 3584
XmnI GAANNNNTTC 5 cut(s) 1248, 2256, 2554, 2922, 3402
Zsp2I ATGCAT 3 cut(s) 131, 1381, 3478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.