MD13G1167100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010154
Physical Location & Seq
Reverse (-)
13519811 .. 13525918
6108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1167100.v1.1.491

Sequence Viewer

Length: 3861 bp
ATGGAAGCTAGCTCTTCTTCTGCTCCAATGGTTTCGGGTTCGGTTTCATCCCACGATCAAGAGAGACTGAACTTGAAATCCATATGGGGGGTTGTCGTTTCGGTCAACAACTTCATCCACAAACTCGTATCAAATGCCAAAACCAGAAACTCTATCCGGATGAGATGCGCCACCAAGTTTACTACTGCTCAAAAACACAAATTCTTTGAGTTCTCTGAGCAGTCTGTGTTATCGAACTTTTACTGGGGCATTGACAGCATCGAAGCCGCAATTCAAGAAAAGTGTTTGGATGAGAAAGCAGCTCTGCTGAAGAAGGCAGAGCAAATGCTGCAGGTTCCCGCGCTGCTCGACGAGCACGGTGTCACAGCTGGAATTCACAACCGGTATTTGGTCTGCTGCTCTTACTTTTACCTCTCATTGGTCAGGAAGCTCCAGGAGGACGAGTGGCAGGTTGCGCTGCATTTCCTGCAGGCAGTGCTGGTGTCCCCGGGGCTTGTTCAAACAGAATTCGCACCAGAACTCTGCGAAAGGCTCTTCCCATCACGCACCGCCAAGTGTGGGAAGCAAGCAACAAGAGGGAGGGGGAACGCGGGGTCAATTTTTCCCTACGTGGAACATGAGGAAGATGAAGCAATAGGGCGGATGGCGAGGGTGTGGAGAGATTGGCTGATGTATTATCAGGTCATGTTGTACGGAGAGACTCCTCGGTGGGAATGTGACACTGAGCCTCAATATTCTATGCATGAAAAATCTACCAGCTCGTTTTCTTCGAGTACAATTGAGCACTGGGAGCATGTAACGGTGCACCCTTTTGATCCTCGAGAGGATATTGGCGGTGGCATGGGAGATGAAATGAAGACTTCTTCCATCTACATCCGGGAATTTGAAGAATATGAGAAACCTGCAAAGGATTTATATCAAGTGACAAGTTTTAATGTCGAGAAGCTTCAAAGGGGCTCAAGTATCAAAAGACTCCACGATATGTTGAATGACTCGCAATCTGATATGCGGACTTCAGTGGGTTCATGCTCCGATTTTTCTGCTTATGACATTGAGTCAGAGGTCATGGATTATGGCGAATGTTCACTGACAACCACAAGAACTAGTGCGGATTTTCCCAGGTCAGAAATTTGTGACTGGAAGCTGCAGGAGTCAGTGCGTAAAGAAGTCCTGGAAGTAAATAACTCTGCGTTACTTGCCAGCGGATTCGCCAGTTCAATCAAAAGTTTAAATTTAACCCTGTTCGAACACAGAGATAAAAAATCAAAGACCTTTGGGCAGCATTGTATGATCAACCAGATGGATCACCAACAGAGTGGTGCACGAAGAAAGCATAATTTTCGTGGCCACGAAGTAGGTTCACACCCTGCAAAAGACTCCAAAAGTGAACTATTTCATATGACCGAAATGGCAATCTCAAAATTATTGTACTCGGAAGGATTAGGAAAATGGGAGGAAGATCATGTTGTAGAAGTGACAACAATATACGAAATATTGGGCAAAAAGAATGGAGAAAACTGCGCTGTCTTGAAAGACATGATCTTGGATCAATTGCTAGCAGGCATTTCTAGTTCGACAGAGGACATTGTTATAAGGGCATCAATTTCGATACTAACCAGCATTGCTGCAGCAAATAAGTCGGTTATAGAAGATGTCAAGAAGAAAGGTCTGCAGCTGTCTGATCTGGCAAGTGCTCTCAAAAGAAATGTGCAGGAGGCAGCAGTACTATTTTATTTAATGAATCCAACTCCTGCAGAAATTAAGACTTTTGAACTTTTACCTACATTGGCGGGGATCATGTGCACTTCGAATAGTTACAAGTGTAGGTCAGGATCACTGCCGTCTCCTATGACAGCATGCCTGATGATTACTGAAATTTTAGTAACCGCATTCGACCATGCCACGAACAACGTGCACTTGGCTGAAATCAGTTCTCCTAATGTTCTTGAAGGGCTCCTAGATGTTGCAAGGGACAGCAACATTGAAGAATTGATCTCCTTGGCAACTATTCTTGTAAAATGCATACAATATGACGGAGATAGCCGAAGATATATATCGAAATTAGCTCCTGTGGCTCCATTTGTCCGGCTTCTGGAAAGTAACAACAAGCATGCCAAGTTCATAGCACTCGAATTTTTCCATCATGCTCTTTGCATGCCACGGTCATCAACTATTACCTTGCTGAAGCGGCTACAACGCGAAGGAAACACGAACATTGTGAACTCACTCATGCTGTGTATCAAAGAATTTGAACCCGAATACCAAGTTTTGGCAGCAAATTTGCTGCTTCATTTAGATACACTAGATAACTCCACTGGTAAGAGTATATTCAGAGAAGAAGCAGTGCAGGTTCTTCTCAAGTCAGTGGCATCTGAAGAAAGCTGTGGTGCACAACAATTATCTGCATTCATTATATCGAATCTTGGTGGAACATATTCTTGGACAGGGGAACCATACACAATGGGATGGTTGGTTAAAAAAGCATGCTTGACTTCCTCGTATCAACGAAATATGATCAAGAACATTTGTTGGTCGGATGATTGCTTAGAGGATGCTGGAACAGACTCATGGTGCATCAAAATTGCCAGAAGCATCATTAACATTGGGAATCCGGTTTTCCATTCGCTAGCAAAGGGCCTAAAGAGTACAATAAGGAGTGTTTCAAGGGATTGTCTGACAACTGTTGCATGGCTTGGATTTGAAATAGCGAAGAGTCCAGAGAGCGTAAAATATACTGCTTGTGAGATCTTGCTTAGCGGAGTTGAGCAATTTTTGCATCCGGGAATGGAGCTAGAAGAAAGAGTTCTAGCATGTCTCTGCATATACAACTATGCTTCTGGTAAAGGGATGAAAAAATTAGTTCATTTCTCGGAAGGAGTACGAGAATCACTAAGACGCTTGTCAAGTGTAACATGGATGGCGGAAGAATTACATAAAGTAGCTGATTATGTTCAGCCAAATTTATCACGTATTTCATGTGTTCACACACAAATCCTTGAGGTCGGTGTCATTTGCAGTGGAGCTGTATGTGCCCTCATCTATTACAAGGGATTTCTTTGCAGTGGATACTCAGATGGTTCTATTAAGGTATGGAACATCAAGGGGCAATCAGCCACCCTTGTATGGGACGTGAAAGAGCACAAAAAGGCAGTTACGTGTTTTTCGCTTGTTGAACCGGGGGACAGCCTTATAAGCGGATCACTGGACAAAACCATTAGGGTCTGGCAAGTGGTTCACAGGAAATTGGAGTGCATTGAGGTGATATCAGCAAAGAAACCAGTTCAGCACCTTAATACGCATGGTCGAACGATATTTGCAACTACCAATGGCCATGGAATTAAGGTGTTTGATGCATCAAGAAAGGCCAAATATAATTGCAAGAGTAAAAAGGTGAAGTGCATGGCAGTGGTTCAAGGGAAGATTTTTGCAGGGTGCAAGGACTCAAGCATACAGGAGTTTTCCATCATGAACAACCGGGCGCAGGAGATCAAAGCAGCAAGGAAATTCTGGAAGCTGCAAAAAAGGCCTATCACTGCAATTGTAACATACAAAGATTGGCTTTACAGTGCAAGTTCGATCGTGGAAGGTTCAAATTTGAAGGAATGGAGAAGACACATCAGACCCCAAATGTCACTAAAAACCGGGAAAAGAGAACGAGTAAAGGGAATGGGAATAGTGGAAGACTTCATATACCTTAACTGCAGCTCAGCAACAAACTGCATTCAGATATGGTTAAGAGGATCACAGCAGAAAGTGGGAAGAATTTCAGCAGGAAGTAGAATCACCAGCCTACTCACAGCAAACGACATTATCTTATGTGGTACCGAGACAGGATTAATCAAGGGCTGGATCCCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1287

Amino Acids

144.08

Weight (kDa)

8.13

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 31 - 181 4.9e-25 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 464 - 624 1.9e-44 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 627 - 980 3.6e-57 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_CDC20-Fz PF24807 965 - 1081 4.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1009 - 1110 4.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 1018 - 1155 1.4e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1018 - 1121 1.7e-11 WDR5 beta-propeller domain
WDR55 PF24796 1018 - 1157 1.3e-08 WDR55
WD40_Prp19 PF24814 1018 - 1156 9.6e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 1019 - 1128 3.3e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1592, 3191
Acc36I ACCTGC 4 cut(s) 322, 439, 910, 2338
Acc65I GGTACC 1 cut(s) 3821
AccB1I GGYRCC 1 cut(s) 3821
AccB7I CCANNNNNTGG 1 cut(s) 2269
AccII CGCG 3 cut(s) 341, 590, 2199
AccIII TCCGGA 1 cut(s) 156
AclWI GGATC 9 cut(s) 809, 1311, 1554, 1802, 1840, 3205, 3748, 3844, 3857
AcoI YGGCCR 2 cut(s) 1345, 3328
AcuI CTGAAG 4 cut(s) 329, 999, 2204, 2394
AfaI GTAC 7 cut(s) 692, 775, 1430, 1725, 2647, 2880, 3823
AfiI CCNNNNNNNGG 9 cut(s) 87, 388, 418, 558, 2092, 2269, 3123, 3124, 3481
AflIII ACRYGT 1 cut(s) 3155
AgeI ACCGGT 1 cut(s) 381
AhdI GACNNNNNGTC 1 cut(s) 1054
AhlI ACTAGT 1 cut(s) 1103
AjiI CACGTC 1 cut(s) 3130
AjnI CCWGG 3 cut(s) 432, 1118, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 2422, 2454
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
Alw21I GWGCWC 9 cut(s) 357, 786, 807, 1324, 1696, 1805, 1917, 2392, 3141
Alw26I GTCTC 5 cut(s) 58, 692, 1848, 2819, 3821
Alw44I GTGCAC 5 cut(s) 803, 1320, 1801, 1913, 2388
AlwI GGATC 9 cut(s) 809, 1311, 1554, 1802, 1840, 3205, 3748, 3844, 3857
Ama87I CYCGRG 2 cut(s) 487, 819
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 5 cut(s) 1345, 2635, 3328, 3363, 3524
ApaLI GTGCAC 5 cut(s) 803, 1320, 1801, 1913, 2388
AseI ATTAAT 1 cut(s) 3836
AsiGI ACCGGT 1 cut(s) 381
Asp700I GAANNNNTTC 4 cut(s) 1392, 2434, 2802, 3763
Asp718I GGTACC 1 cut(s) 3821
AspLEI GCGC 5 cut(s) 170, 343, 457, 1523, 3481
AspS9I GGNCC 1 cut(s) 2635
AsuC2I CCSGG 7 cut(s) 488, 489, 878, 2781, 3177, 3476, 3643
AsuHPI GGTGA 4 cut(s) 1298, 3271, 3403, 3775
AsuII TTCGAA 2 cut(s) 1245, 1808
AsuNHI GCTAGC 3 cut(s) 8, 1555, 2626
AvaI CYCGRG 2 cut(s) 487, 819
BaeGI GKGCMC 6 cut(s) 807, 1324, 1805, 1917, 2392, 3034
BalI TGGCCA 2 cut(s) 1347, 3330
BamHI GGATCC 1 cut(s) 3849
BanI GGYRCC 1 cut(s) 3821
BanII GRGCYC 2 cut(s) 959, 1956
BbsI GAAGAC 3 cut(s) 863, 3616, 3687
Bbv12I GWGCWC 9 cut(s) 357, 786, 807, 1324, 1696, 1805, 1917, 2392, 3141
BccI CCATC 9 cut(s) 547, 637, 875, 1294, 2148, 2460, 2911, 3068, 3470
BceAI ACGGC 1 cut(s) 1825
BciT130I CCWGG 3 cut(s) 434, 1120, 1172
BciVI GTATCC 1 cut(s) 3059
BclI TGATCA 2 cut(s) 1290, 2514
BcnI CCSGG 7 cut(s) 488, 489, 878, 2781, 3177, 3476, 3643
BcoDI GTCTC 5 cut(s) 58, 692, 1848, 2819, 3821
BcuI ACTAGT 1 cut(s) 1103
BfaI CTAG 9 cut(s) 9, 1104, 1556, 1569, 1958, 2303, 2627, 2792, 2807
BfmI CTRYAG 7 cut(s) 329, 467, 1145, 1626, 1670, 1752, 3700
BfuAI ACCTGC 4 cut(s) 322, 439, 910, 2338
BfuI GTATCC 1 cut(s) 3059
BglII AGATCT 1 cut(s) 2745
BlpI GCTNAGC 2 cut(s) 2753, 3706
BmcAI AGTACT 1 cut(s) 1725
BmeRI GACNNNNNGTC 1 cut(s) 1054
BmeT110I CYCGRG 2 cut(s) 487, 819
BmgBI CACGTC 1 cut(s) 3130
BmgT120I GGNCC 1 cut(s) 2635
BmiI GGNNCC 6 cut(s) 336, 1955, 2076, 2451, 3823, 3851
BmrI ACTGGG 2 cut(s) 253, 796
BmtI GCTAGC 3 cut(s) 12, 1559, 2630
BmuI ACTGGG 2 cut(s) 253, 796
BoxI GACNNNNGTC 1 cut(s) 2899
BpiI GAAGAC 3 cut(s) 863, 3616, 3687
BpmI CTGGAG 1 cut(s) 416
Bpu1102I GCTNAGC 2 cut(s) 2753, 3706
Bpu14I TTCGAA 2 cut(s) 1245, 1808
BpuEI CTTGAG 4 cut(s) 943, 2342, 3017, 3427
BpuMI CCSGG 7 cut(s) 488, 489, 878, 2781, 3177, 3476, 3643
BsaAI YACGTR 3 cut(s) 610, 2969, 3156
BsaBI GATNNNNATC 2 cut(s) 915, 2053
BsaJI CCNNGG 9 cut(s) 486, 487, 488, 704, 1118, 1998, 2159, 3176, 3331
BsaWI WCCGGW 3 cut(s) 156, 381, 2611
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 388, 418, 558, 2092, 2269, 3123, 3124, 3481
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 7 cut(s) 248, 791, 1142, 1212, 2320, 3207, 3278
Bse3DI GCAATG 1 cut(s) 1620
Bse8I GATNNNNATC 2 cut(s) 915, 2053
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 3 cut(s) 434, 1120, 1172
BseDI CCNNGG 9 cut(s) 486, 487, 488, 704, 1118, 1998, 2159, 3176, 3331
BseJI GATNNNNATC 2 cut(s) 915, 2053
BseLI CCNNNNNNNGG 9 cut(s) 87, 388, 418, 558, 2092, 2269, 3123, 3124, 3481
BseMI GCAATG 1 cut(s) 1620
BseMII CTCAG 4 cut(s) 207, 714, 3084, 3720
BseNI ACTGG 7 cut(s) 248, 791, 1142, 1212, 2320, 3207, 3278
BseRI GAGGAG 1 cut(s) 693
BseSI GKGCMC 6 cut(s) 807, 1324, 1805, 1917, 2392, 3034
BsgI GTGCAG 2 cut(s) 1730, 2366
Bsh1236I CGCG 3 cut(s) 341, 590, 2199
Bsh1285I CGRYCG 1 cut(s) 3579
BshFI GGCC 5 cut(s) 1347, 2637, 3330, 3365, 3526
BshNI GGYRCC 1 cut(s) 3821
BshTI ACCGGT 1 cut(s) 381
BsiEI CGRYCG 1 cut(s) 3579
BsiHKAI GWGCWC 9 cut(s) 357, 786, 807, 1324, 1696, 1805, 1917, 2392, 3141
BsiHKCI CYCGRG 2 cut(s) 487, 819
BslFI GGGAC 4 cut(s) 469, 1985, 3140, 3194
BslI CCNNNNNNNGG 9 cut(s) 87, 388, 418, 558, 2092, 2269, 3123, 3124, 3481
BsmAI GTCTC 5 cut(s) 58, 692, 1848, 2819, 3821
BsmBI CGTCTC 1 cut(s) 1848
BsmFI GGGAC 4 cut(s) 469, 1985, 3140, 3194
BsmI GAATGC 3 cut(s) 1889, 2405, 3720
BsnI GGCC 5 cut(s) 1347, 2637, 3330, 3365, 3526
BsoBI CYCGRG 2 cut(s) 487, 819
Bsp119I TTCGAA 2 cut(s) 1245, 1808
Bsp13I TCCGGA 1 cut(s) 156
Bsp1720I GCTNAGC 2 cut(s) 2753, 3706
Bsp19I CCATGG 1 cut(s) 3331
BspANI GGCC 5 cut(s) 1347, 2637, 3330, 3365, 3526
BspCNI CTCAG 4 cut(s) 208, 715, 3083, 3719
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 3 cut(s) 341, 590, 2199
BspHI TCATGA 1 cut(s) 3465
BspLI GGNNCC 6 cut(s) 336, 1955, 2076, 2451, 3823, 3851
BspMAI CTGCAG 7 cut(s) 333, 471, 1149, 1630, 1674, 1756, 3704
BspMI ACCTGC 4 cut(s) 322, 439, 910, 2338
BspOI GCTAGC 3 cut(s) 12, 1559, 2630
BspPI GGATC 9 cut(s) 809, 1311, 1554, 1802, 1840, 3205, 3748, 3844, 3857
BspQI GCTCTTC 2 cut(s) 19, 539
BspT104I TTCGAA 2 cut(s) 1245, 1808
BspT107I GGYRCC 1 cut(s) 3821
BsrDI GCAATG 1 cut(s) 1620
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 7 cut(s) 248, 791, 1142, 1212, 2320, 3207, 3278
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 9 cut(s) 486, 487, 488, 704, 1118, 1998, 2159, 3176, 3331
BssT1I CCWWGG 2 cut(s) 1998, 3331
Bst2UI CCWGG 3 cut(s) 434, 1120, 1172
Bst4CI ACNGT 5 cut(s) 359, 802, 2163, 2683, 3566
Bst6I CTCTTC 3 cut(s) 19, 539, 2705
BstAPI GCANNNNNTGC 4 cut(s) 328, 466, 475, 2773
BstBAI YACGTR 3 cut(s) 610, 2969, 3156
BstBI TTCGAA 2 cut(s) 1245, 1808
BstDEI CTNAG 7 cut(s) 216, 723, 2545, 2753, 2891, 3070, 3706
BstDSI CCRYGG 2 cut(s) 2159, 3331
BstFNI CGCG 3 cut(s) 341, 590, 2199
BstHHI GCGC 5 cut(s) 170, 343, 457, 1523, 3481
BstMAI GTCTC 5 cut(s) 58, 692, 1848, 2819, 3821
BstMCI CGRYCG 1 cut(s) 3579
BstNI CCWGG 3 cut(s) 434, 1120, 1172
BstNSI RCATGY 6 cut(s) 797, 1860, 2114, 2158, 2487, 2814
BstPAI GACNNNNGTC 1 cut(s) 2899
BstSFI CTRYAG 7 cut(s) 329, 467, 1145, 1626, 1670, 1752, 3700
BstSLI GKGCMC 6 cut(s) 807, 1324, 1805, 1917, 2392, 3034
BstUI CGCG 3 cut(s) 341, 590, 2199
BstV2I GAAGAC 3 cut(s) 863, 3616, 3687
BstX2I RGATCY 2 cut(s) 2745, 3849
BstXI CCANNNNNNTGG 1 cut(s) 1316
BstYI RGATCY 2 cut(s) 2745, 3849
BsuI GTATCC 1 cut(s) 3059
BsuRI GGCC 5 cut(s) 1347, 2637, 3330, 3365, 3526
BtgI CCRYGG 2 cut(s) 2159, 3331
BtrI CACGTC 1 cut(s) 3130
BtsI GCAGTG 7 cut(s) 480, 1835, 2349, 3022, 3067, 3411, 3531
BveI ACCTGC 4 cut(s) 322, 439, 910, 2338
CciI TCATGA 1 cut(s) 3465
CfoI GCGC 5 cut(s) 170, 343, 457, 1523, 3481
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 2635
Cfr9I CCCGGG 1 cut(s) 487
CseI GACGC 1 cut(s) 2904
Csp6I GTAC 7 cut(s) 691, 774, 1429, 1724, 2646, 2879, 3822
CspAI ACCGGT 1 cut(s) 381
CviQI GTAC 7 cut(s) 691, 774, 1429, 1724, 2646, 2879, 3822
DdeI CTNAG 7 cut(s) 216, 723, 2545, 2753, 2891, 3070, 3706
DraI TTTAAA 1 cut(s) 1230
DriI GACNNNNNGTC 1 cut(s) 1054
EaeI YGGCCR 2 cut(s) 1345, 3328
Eam1104I CTCTTC 3 cut(s) 19, 539, 2705
Eam1105I GACNNNNNGTC 1 cut(s) 1054
EarI CTCTTC 3 cut(s) 19, 539, 2705
EciI GGCGGA 2 cut(s) 655, 2936
Eco130I CCWWGG 2 cut(s) 1998, 3331
Eco147I AGGCCT 1 cut(s) 3526
Eco24I GRGCYC 2 cut(s) 959, 1956
Eco32I GATATC 1 cut(s) 3264
Eco57I CTGAAG 4 cut(s) 329, 999, 2204, 2394
Eco88I CYCGRG 2 cut(s) 487, 819
EcoO109I RGGNCCY 1 cut(s) 2635
EcoRI GAATTC 2 cut(s) 372, 506
EcoRII CCWGG 3 cut(s) 432, 1118, 1170
EcoRV GATATC 1 cut(s) 3264
EcoT14I CCWWGG 2 cut(s) 1998, 3331
EcoT22I ATGCAT 3 cut(s) 744, 2024, 3355
EcoT38I GRGCYC 2 cut(s) 959, 1956
ErhI CCWWGG 2 cut(s) 1998, 3331
Esp3I CGTCTC 1 cut(s) 1848
FalI AAGNNNNNCTT 2 cut(s) 3543, 3575
FaqI GGGAC 4 cut(s) 469, 1985, 3140, 3194
FauI CCCGC 3 cut(s) 346, 583, 1783
FauNDI CATATG 2 cut(s) 83, 1398
FbaI TGATCA 2 cut(s) 1290, 2514
FriOI GRGCYC 2 cut(s) 959, 1956
FspBI CTAG 9 cut(s) 9, 1104, 1556, 1569, 1958, 2303, 2627, 2792, 2807
GlaI GCGC 5 cut(s) 169, 342, 456, 1522, 3480
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 5 cut(s) 1347, 2637, 3330, 3365, 3526
HgaI GACGC 1 cut(s) 2904
HhaI GCGC 5 cut(s) 170, 343, 457, 1523, 3481
Hin6I GCGC 5 cut(s) 168, 341, 455, 1521, 3479
HinP1I GCGC 5 cut(s) 168, 341, 455, 1521, 3479
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 944
HphI GGTGA 4 cut(s) 1298, 3271, 3403, 3775
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 7 cut(s) 307, 1430, 1943, 2195, 2867, 3578, 3592
HpyCH4III ACNGT 5 cut(s) 359, 802, 2163, 2683, 3566
HpyCH4IV ACGT 5 cut(s) 609, 1911, 2968, 3129, 3155
HpyF3I CTNAG 7 cut(s) 216, 723, 2545, 2753, 2891, 3070, 3706
HpySE526I ACGT 5 cut(s) 609, 1911, 2968, 3129, 3155
HspAI GCGC 5 cut(s) 168, 341, 455, 1521, 3479
Kpn2I TCCGGA 1 cut(s) 156
KpnI GGTACC 1 cut(s) 3825
Ksp22I TGATCA 2 cut(s) 1290, 2514
LguI GCTCTTC 2 cut(s) 19, 539
LmnI GCTCC 9 cut(s) 28, 435, 790, 1034, 1959, 2071, 2080, 2788, 3020
MaeI CTAG 9 cut(s) 9, 1104, 1556, 1569, 1958, 2303, 2627, 2792, 2807
MaeII ACGT 5 cut(s) 609, 1911, 2968, 3129, 3155
MfeI CAATTG 3 cut(s) 777, 1550, 3537
MflI RGATCY 2 cut(s) 2745, 3849
MlsI TGGCCA 2 cut(s) 1347, 3330
MluNI TGGCCA 2 cut(s) 1347, 3330
MlyI GAGTC 9 cut(s) 694, 966, 986, 1064, 1160, 1370, 2558, 2722, 3434
MmeI TCCRAC 2 cut(s) 1769, 2514
Mox20I TGGCCA 2 cut(s) 1347, 3330
Mph1103I ATGCAT 3 cut(s) 744, 2024, 3355
MroI TCCGGA 1 cut(s) 156
MroXI GAANNNNTTC 4 cut(s) 1392, 2434, 2802, 3763
MscI TGGCCA 2 cut(s) 1347, 3330
MslI CAYNNNNRTG 2 cut(s) 3257, 3404
Msp20I TGGCCA 2 cut(s) 1347, 3330
MspA1I CMGCKG 3 cut(s) 368, 1203, 1675
MunI CAATTG 3 cut(s) 777, 1550, 3537
Mva1269I GAATGC 3 cut(s) 1889, 2405, 3720
MvaI CCWGG 3 cut(s) 434, 1120, 1172
MvnI CGCG 3 cut(s) 341, 590, 2199
NciI CCSGG 7 cut(s) 488, 489, 878, 2781, 3177, 3476, 3643
NcoI CCATGG 1 cut(s) 3331
NdeI CATATG 2 cut(s) 83, 1398
NheI GCTAGC 3 cut(s) 8, 1555, 2626
NlaIV GGNNCC 6 cut(s) 336, 1955, 2076, 2451, 3823, 3851
NmuCI GTSAC 6 cut(s) 361, 716, 922, 1133, 1474, 3630
NsiI ATGCAT 3 cut(s) 744, 2024, 3355
NspI RCATGY 6 cut(s) 797, 1860, 2114, 2158, 2487, 2814
NspV TTCGAA 2 cut(s) 1245, 1808
PaeI GCATGC 4 cut(s) 1860, 2114, 2158, 2487
PaeR7I CTCGAG 1 cut(s) 819
PagI TCATGA 1 cut(s) 3465
PceI AGGCCT 1 cut(s) 3526
PciSI GCTCTTC 2 cut(s) 19, 539
PcsI WCGNNNNNNNCGW 1 cut(s) 767
PctI GAATGC 3 cut(s) 1889, 2405, 3720
PdmI GAANNNNTTC 4 cut(s) 1392, 2434, 2802, 3763
PfeI GAWTC 6 cut(s) 1206, 1741, 2419, 2608, 2885, 3780
PflMI CCANNNNNTGG 1 cut(s) 2269
PfoI TCCNGGA 4 cut(s) 432, 876, 1170, 2779
PinAI ACCGGT 1 cut(s) 381
Ple19I CGATCG 1 cut(s) 3579
PleI GAGTC 9 cut(s) 694, 966, 986, 1063, 1159, 1370, 2558, 2721, 3434
PpsI GAGTC 9 cut(s) 694, 966, 986, 1063, 1159, 1370, 2558, 2721, 3434
Ppu21I YACGTR 3 cut(s) 610, 2969, 3156
PshAI GACNNNNGTC 1 cut(s) 2899
PshBI ATTAAT 1 cut(s) 3836
PsiI TTATAA 2 cut(s) 1592, 3191
Psp6I CCWGG 3 cut(s) 432, 1118, 1170
PspGI CCWGG 3 cut(s) 432, 1118, 1170
PspN4I GGNNCC 6 cut(s) 336, 1955, 2076, 2451, 3823, 3851
PspPI GGNCC 1 cut(s) 2635
PstI CTGCAG 7 cut(s) 333, 471, 1149, 1630, 1674, 1756, 3704
PsuI RGATCY 2 cut(s) 2745, 3849
PvuI CGATCG 1 cut(s) 3579
PvuII CAGCTG 2 cut(s) 368, 1675
RsaI GTAC 7 cut(s) 692, 775, 1430, 1725, 2647, 2880, 3823
RsaNI GTAC 7 cut(s) 691, 774, 1429, 1724, 2646, 2879, 3822
RseI CAYNNNNRTG 2 cut(s) 3257, 3404
SapI GCTCTTC 2 cut(s) 19, 539
Sau96I GGNCC 1 cut(s) 2635
SbfI CCTGCAGG 1 cut(s) 471
ScaI AGTACT 1 cut(s) 1725
SchI GAGTC 9 cut(s) 694, 966, 986, 1064, 1160, 1370, 2558, 2722, 3434
SdaI CCTGCAGG 1 cut(s) 471
SfcI CTRYAG 7 cut(s) 329, 467, 1145, 1626, 1670, 1752, 3700
Sfr274I CTCGAG 1 cut(s) 819
SfuI TTCGAA 2 cut(s) 1245, 1808
SlaI CTCGAG 1 cut(s) 819
SmaI CCCGGG 1 cut(s) 489
SmiMI CAYNNNNRTG 2 cut(s) 3257, 3404
SmlI CTYRAG 5 cut(s) 819, 958, 2357, 2996, 3442
SmoI CTYRAG 5 cut(s) 819, 958, 2357, 2996, 3442
SpeI ACTAGT 1 cut(s) 1103
SphI GCATGC 4 cut(s) 1860, 2114, 2158, 2487
Sse8387I CCTGCAGG 1 cut(s) 471
SseBI AGGCCT 1 cut(s) 3526
SspI AATATT 2 cut(s) 734, 1494
SspMI CTAG 9 cut(s) 9, 1104, 1556, 1569, 1958, 2303, 2627, 2792, 2807
StuI AGGCCT 1 cut(s) 3526
StyI CCWWGG 2 cut(s) 1998, 3331
TaaI ACNGT 5 cut(s) 359, 802, 2163, 2683, 3566
TaiI ACGT 5 cut(s) 612, 1914, 2971, 3132, 3158
TaqII GACCGA 2 cut(s) 91, 1418
TatI WGTACW 4 cut(s) 773, 1428, 1723, 2645
TauI GCSGC 2 cut(s) 269, 2191
TfiI GAWTC 6 cut(s) 1206, 1741, 2419, 2608, 2885, 3780
TseFI GTSAC 6 cut(s) 361, 716, 922, 1133, 1474, 3630
Tsp45I GTSAC 6 cut(s) 361, 716, 922, 1133, 1474, 3630
TspGWI ACGGA 2 cut(s) 708, 2049
TspMI CCCGGG 1 cut(s) 487
Van91I CCANNNNNTGG 1 cut(s) 2269
VneI GTGCAC 5 cut(s) 803, 1320, 1801, 1913, 2388
VspI ATTAAT 1 cut(s) 3836
XceI RCATGY 6 cut(s) 797, 1860, 2114, 2158, 2487, 2814
XhoI CTCGAG 1 cut(s) 819
XmaI CCCGGG 1 cut(s) 487
XmnI GAANNNNTTC 4 cut(s) 1392, 2434, 2802, 3763
XspI CTAG 9 cut(s) 9, 1104, 1556, 1569, 1958, 2303, 2627, 2792, 2807
ZrmI AGTACT 1 cut(s) 1725
Zsp2I ATGCAT 3 cut(s) 744, 2024, 3355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.