Rorug04G0181700

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010154
Physical Location & Seq
Forward (+)
31806877 .. 31808327
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0181700.1

Sequence Viewer

Length: 381 bp
ATGTACAACGGAATAGGGTTACAGACTCCGAGAGGGTCTGGAACCAATGGCAATATCCAGACGAACAAGTTCTTCATCAGGTCCAAGACTGGCAAGGTCGCTGAAAACTCCAGAGGGTTTGAGGGAGACCAGGGCATGGCTGGCGTCACAAAGAAGGCCAACAGAGACATCCTTGAGCATGATCGCAAGCGCCAGATTGAGCTCAAGCTTGTGGTGCTGGAGGAGAATCTGAGCGATCAGGGATTCACGGAGGCTGAGATTGGTGAGAAGGTCGCCGAGGCTCGGAGGACTCTGGAAGCTGCTGCCGCTTCTGAGGAGATTGGTGGAACCACTGCTATTGTTCTTGGAGATAAGAAGTTACATGGCCTCAAAGCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

126

Amino Acids

13.65

Weight (kDa)

7.99

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 100 8.4e-16 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 136
AciI CCGC 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 136, 282
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 4 cut(s) 202, 208, 299, 374
AluI AGCT 4 cut(s) 202, 208, 299, 374
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 120, 159
AoxI GGCC 2 cut(s) 156, 364
ApeKI GCWGC 2 cut(s) 299, 302
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 275
AvaII GGWCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 286, 289
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 120, 159
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 3 cut(s) 300, 303, 306
BlsI GCNGC 3 cut(s) 301, 304, 307
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 43, 328
BmrFI CCNGG 1 cut(s) 131
BpmI CTGGAG 2 cut(s) 94, 239
BpuEI CTTGAG 2 cut(s) 188, 194
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 130, 276
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 282
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 130, 276
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 136, 282
BseMII CTCAG 3 cut(s) 221, 246, 303
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 236, 329
BseXI GCAGC 2 cut(s) 286, 289
BshFI GGCC 2 cut(s) 158, 366
BsiHKAI GWGCWC 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 136, 282
BsmAI GTCTC 2 cut(s) 120, 159
BsnI GGCC 2 cut(s) 158, 366
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 181, 235
BspACI CCGC 1 cut(s) 306
BspANI GGCC 2 cut(s) 158, 366
BspCNI CTCAG 3 cut(s) 222, 247, 304
BspLI GGNNCC 2 cut(s) 43, 328
BspTNI GGTCTC 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 130, 276
BssMI GATC 2 cut(s) 181, 235
BssNI GRCGYC 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 144
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 142, 188
BstDEI CTNAG 3 cut(s) 230, 255, 312
BstF5I GGATG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 2 cut(s) 184, 238
BstMAI GTCTC 2 cut(s) 120, 159
BstMBI GATC 2 cut(s) 181, 235
BstMWI GCNNNNNNNGC 3 cut(s) 141, 214, 305
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 286, 289
BsuRI GGCC 2 cut(s) 158, 366
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 142, 188
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 3 cut(s) 136, 179, 362
CviJI RGCY 9 cut(s) 140, 158, 202, 208, 254, 281, 299, 366, 374
CviKI_1 RGCY 9 cut(s) 140, 158, 202, 208, 254, 281, 299, 366, 374
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 3 cut(s) 230, 255, 312
DpnI GATC 2 cut(s) 183, 237
DpnII GATC 2 cut(s) 181, 235
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 204
FaeI CATG 3 cut(s) 139, 182, 365
FaiI YATR 3 cut(s) 137, 180, 363
FatI CATG 3 cut(s) 135, 178, 361
Fnu4HI GCNGC 3 cut(s) 300, 303, 306
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 300, 303, 306
GlaI GCGC 1 cut(s) 191
GluI GCNGC 3 cut(s) 300, 303, 306
GsuI CTGGAG 2 cut(s) 94, 239
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 2 cut(s) 158, 366
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 139, 182, 365
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 2 cut(s) 206, 372
HinfI GANTC 4 cut(s) 25, 226, 243, 289
HphI GGTGA 1 cut(s) 275
Hpy188I TCNGA 4 cut(s) 30, 231, 285, 313
Hpy188III TCNNGA 4 cut(s) 39, 58, 111, 293
HpyAV CCTTC 2 cut(s) 148, 262
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 214, 305
HpyF3I CTNAG 3 cut(s) 230, 255, 312
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 139, 182, 365
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 181, 235
Lsp1109I GCAGC 2 cut(s) 286, 289
MaeIII GTNAC 3 cut(s) 18, 145, 357
MalI GATC 2 cut(s) 183, 237
MboI GATC 2 cut(s) 181, 235
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 19, 283
MnlI CCTC 9 cut(s) 26, 107, 115, 214, 244, 271, 279, 307, 377
MroXI GAANNNNTTC 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 3 cut(s) 141, 214, 305
NdeII GATC 2 cut(s) 181, 235
NlaIII CATG 3 cut(s) 139, 182, 365
NlaIV GGNNCC 2 cut(s) 43, 328
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 226, 243
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 3 cut(s) 301, 304, 307
PleI GAGTC 2 cut(s) 19, 283
PpsI GAGTC 2 cut(s) 19, 283
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 43, 328
PspPI GGNCC 1 cut(s) 81
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 1 cut(s) 204
SatI GCNGC 3 cut(s) 300, 303, 306
Sau3AI GATC 2 cut(s) 181, 235
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 2 cut(s) 19, 283
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 204
SetI ASST 7 cut(s) 83, 99, 204, 210, 273, 301, 376
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 173, 203
SmoI CTYRAG 2 cut(s) 173, 203
SsiI CCGC 1 cut(s) 306
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 129
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 226, 243
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 299, 302
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 2 cut(s) 24, 263
TspRI CASTG 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.